CN106990090B - Device and method for dynamic surface enhanced Raman spectroscopy detection - Google Patents
Device and method for dynamic surface enhanced Raman spectroscopy detection Download PDFInfo
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- CN106990090B CN106990090B CN201710237085.9A CN201710237085A CN106990090B CN 106990090 B CN106990090 B CN 106990090B CN 201710237085 A CN201710237085 A CN 201710237085A CN 106990090 B CN106990090 B CN 106990090B
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- 238000001514 detection method Methods 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000004416 surface enhanced Raman spectroscopy Methods 0.000 title claims description 18
- 238000001069 Raman spectroscopy Methods 0.000 claims abstract description 22
- 238000000479 surface-enhanced Raman spectrum Methods 0.000 claims abstract description 13
- 230000008569 process Effects 0.000 claims abstract description 12
- 230000003595 spectral effect Effects 0.000 claims abstract description 6
- 238000012545 processing Methods 0.000 claims abstract description 4
- 238000004458 analytical method Methods 0.000 claims abstract description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 27
- 229910052782 aluminium Inorganic materials 0.000 claims description 27
- 238000011156 evaluation Methods 0.000 claims description 15
- 238000002955 isolation Methods 0.000 claims description 11
- 230000008859 change Effects 0.000 claims description 8
- 238000001228 spectrum Methods 0.000 claims description 5
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 4
- 238000004891 communication Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 229910052710 silicon Inorganic materials 0.000 claims description 4
- 239000010703 silicon Substances 0.000 claims description 4
- 230000003287 optical effect Effects 0.000 claims description 3
- 238000011158 quantitative evaluation Methods 0.000 claims description 3
- 238000001237 Raman spectrum Methods 0.000 abstract description 8
- 238000005259 measurement Methods 0.000 abstract description 7
- 238000010586 diagram Methods 0.000 description 6
- 238000004364 calculation method Methods 0.000 description 4
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0289—Field-of-view determination; Aiming or pointing of a spectrometer; Adjusting alignment; Encoding angular position; Size of measurement area; Position tracking
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
Abstract
The invention especially relates to a device for detecting a dynamic surface enhanced Raman spectrum, wherein a confocal Raman spectrometer is positioned above a double-scale mobile detection platform and acquires an image and spectral information of a detected sample placed on the double-scale mobile detection platform, a computing terminal receives the image information of the sample and outputs a control signal to a stepping motor control module after analysis and processing, and the stepping motor control module drives a motor to act so as to realize the focusing of the confocal Raman spectrometer on the detected sample; and discloses a detection method. The computing terminal generates reliable stepping motor control signals according to images acquired on a CCD sensor of the spectrometer, the focusing of the confocal Raman spectrometer on a detected sample is realized through repeated reciprocating control, the continuous focusing in the whole dynamic surface enhanced Raman spectrum measurement successfully solves the continuous and accurate acquisition of the dynamic Raman spectrum, meanwhile, the whole process does not need manual intervention, and is completely automatic and very convenient.
Description
Technical Field
The invention relates to the technical field of Raman spectrum detection, in particular to a device and a method for dynamic surface-enhanced Raman spectrum detection.
Background
The dynamic surface enhanced Raman spectroscopy is a novel detection method developed on the basis of dry and wet surface enhanced Raman spectroscopy, and comprises the specific steps of fully and uniformly mixing an enhanced substrate and a detected object (both of which are liquid), then dripping the mixture on a silicon wafer, and carrying out spectral measurement in a critical state between dry and wet liquid drops. The Raman spectrum obtained in the process has excellent stable reproducibility and better signal intensity, is obviously superior to the traditional method in the aspect of substance detection, and is a detection technology with great application prospect. However, in the actual detection operation, there still remains a problem. Since the measurement is performed in a dry-wet critical state, and the evaporation of the mixed solution causes the position of the liquid level to change, the objective lens or the sample position needs to be adjusted manually and continuously. The manual intervention prevents the dynamic spectrum from being continuously acquired, limits the unique advantage of dynamic surface enhanced Raman spectrum detection to be exerted, and causes more obvious interference on the accurate detection of substances by applying the technology.
Disclosure of Invention
The invention aims to provide a device for detecting a dynamic surface enhanced Raman spectrum, which ensures continuous and accurate acquisition of the dynamic Raman spectrum.
In order to realize the purpose, the invention adopts the technical scheme that: the utility model provides a device for developments surface enhancement raman spectroscopy detects, includes two yardstick mobile detection platform, confocal raman spectrometer, calculation terminal and step motor control module, confocal raman spectrometer be located two yardstick mobile detection platform tops and acquire the image and the spectral information of placing the sample that is surveyed on two yardstick mobile detection platform, calculation terminal receives sample image information and output control signal to step motor control module after the analysis and processing, step motor control module drive two yardstick mobile detection platform in the motor action realize that confocal raman spectrometer is to the focusing of the sample that is surveyed.
Compared with the prior art, the invention has the following technical effects: the computing terminal generates reliable stepping motor control signals according to images acquired on a CCD sensor of the confocal Raman spectrometer, the confocal Raman spectrometer is controlled to focus on a detected sample through repeated reciprocating control, continuous focusing in the whole dynamic surface enhanced Raman spectrum measurement successfully solves continuous and accurate acquisition of a dynamic Raman spectrum, meanwhile, the whole process does not need manual intervention, and the method is completely automatic and very convenient.
Another primary object of the present invention is to provide a detection method for an apparatus for dynamic surface enhanced raman spectroscopy, which ensures continuous and accurate acquisition of dynamic raman spectra.
In order to realize the purpose, the invention adopts the technical scheme that: a method for dynamic surface enhanced Raman spectroscopy detection, comprising the steps of: a: a CCD sensor of the confocal Raman spectrometer acquires an image of a measured sample and outputs the image to a computing terminal; b: the calculation terminal gives a quantitative evaluation value J according to the definition of the image; c: the computer terminal sends a control signal to the stepping motor control module, and the stepping motor control module drives a motor in the dual-scale mobile detection platform to act according to the control signal to adjust the position of the sample to be detected; repeating the step A and the step B, obtaining a new quantized evaluation value J of the definition, continuously adjusting the position of the sample to be measured according to the change of the quantized evaluation value J of the definition until the quantized evaluation value J of the definition is maximum, and finishing focusing; d: starting laser, and cutting off the laser after spectrum collection is completed; e: and repeating the steps A to D until the detection of the single dynamic surface enhanced Raman spectrum is finished.
Compared with the prior art, the invention has the following technical effects: the computing terminal generates reliable stepping motor control signals according to images acquired on a CCD sensor of the confocal Raman spectrometer, the confocal Raman spectrometer is controlled to focus on a detected sample through repeated reciprocating control, continuous focusing in the whole dynamic surface enhanced Raman spectrum measurement successfully solves continuous and accurate acquisition of a dynamic Raman spectrum, meanwhile, the whole process does not need manual intervention, and the method is completely automatic and very convenient.
Drawings
FIG. 1 is a functional block diagram of the present invention;
FIG. 2 is a schematic structural diagram of the dual-scale mobile testing platform of the present invention;
FIG. 3 is a block circuit diagram of the stepper motor control module of the present invention;
FIG. 4 is a circuit diagram of the stepper motor control module of the present invention;
FIG. 5 is a schematic flow diagram of the present invention.
Detailed Description
The present invention will be described in further detail with reference to fig. 1 to 5.
Referring to fig. 1, a device for dynamic surface-enhanced raman spectroscopy detection includes a dual-scale mobile detection platform 10, a confocal raman spectrometer 20, a computing terminal 30 and a stepper motor control module 40, wherein the confocal raman spectrometer 20 is located above the dual-scale mobile detection platform 10 and acquires an image and spectral information of a sample to be detected placed on the dual-scale mobile detection platform 10, and the image information is acquired first, and then the spectral information is acquired after a series of processing and focusing; the computing terminal 30 receives the sample image information, analyzes and processes the sample image information, and then outputs a control signal to the stepping motor control module 40, and the stepping motor control module 40 drives the motor in the dual-scale mobile detection platform 10 to act so as to realize the focusing of the confocal raman spectrometer 20 on the measured sample. The computing terminal 30 generates a reliable stepping motor control signal according to the image obtained on the CCD sensor of the confocal raman spectrometer 20, and the focusing of the confocal raman spectrometer 20 on the measured sample is realized by a plurality of reciprocating controls, so that the continuous focusing in the whole dynamic surface enhanced raman spectroscopy measurement successfully solves the continuous and accurate obtaining of the dynamic raman spectrum, and meanwhile, the whole process does not need manual intervention, is completely automatic, and is very convenient.
Referring to fig. 2, the structure of the dual-scale mobile testing platform 10 is various, in order to ensure the control accuracy, the present invention adopts a stepping motor for control, and other position adjusting devices are also applicable, as long as the position of the tested sample can be accurately and rapidly adjusted, so that the tested sample can be rapidly focused. In this embodiment, the dual-scale mobile detection platform 10 preferably includes a base 11, a rack 12 and a first stepping motor 13 fixed on the base 11, wherein the rack 12 can move up and down along the base 11 and the teeth of the rack 12 are engaged with a toothed disc 131 on the first stepping motor 13; the top end of the rack bar 12 is fixedly provided with a lower component 14, an upper component 15 is arranged above the lower component 14, a silicon wafer 50 containing a sample to be measured is placed on the upper component 15, a spring and a second stepping motor 16 are arranged between the upper component 15 and the lower component 14, the upper component 15 moves downwards under the elastic action of the spring, a cam 161 is arranged on a shaft of the second stepping motor 16, and the distance between the upper component 15 and the lower component 14 can be adjusted when the cam 161 rotates. The first stepping motor 13 and the fluted disc 131 on the first stepping motor are matched with the toothed bar 12, so that the position of the sample to be measured can be quickly adjusted; the position of the sample to be measured is accurately adjusted through the second stepping motor 16 and the cam 161; thus, the focal length can be adjusted in a short time and can be adjusted accurately. The smaller the rise of the cam 161 within 180 °, the more accurate the adjustment.
Preferably, the rise distance of the cam 161 within 180 °, the pitch of the rack bar 12, and the pitch of the toothed disc 131 of the first stepping motor 13 are equal to each other. The adjustment is reasonable, the first stepping motor 13 drives the rack bar 12 to move up and down in a stepped mode, fine adjustment is carried out through the cam 161 after the rack bar 12 moves to a proper position, and the lift distance of the cam 161 is equal to the tooth pitch of the rack bar 12, so that the position with the best focal length can be found in the process that the cam 161 rotates 180 degrees.
The base has various structural modes, and in the embodiment, the base 11 is preferably formed by fixing first, second and third aluminum cross beams 111, 112 and 113 arranged from top to bottom and first, second and third aluminum vertical beams 114, 115 and 116 arranged from left to right through screws or bolts; the first and third aluminum cross beams 111, 113 and the first and third aluminum vertical beams 114, 116 enclose a square frame; the lower end of the second aluminum vertical beam 115 is fixed on the third aluminum cross beam 113, and the upper end of the second aluminum vertical beam 115 is fixedly provided with the first stepping motor 13; two ends of the second aluminum cross beam 112 are respectively fixed on the first and second aluminum vertical beams 114 and 115, and the first and second aluminum cross beams 111 and 112 are respectively provided with through holes for the rack bar 12 to pass through; an upper clamping piece 121 and a lower clamping piece 122 for limiting are arranged on the rod body of the toothed rod 12, and the upper clamping piece 121 and the lower clamping piece 122 are respectively positioned on the upper side and the lower side of the through hole of the first aluminum cross beam 111. The base made of the three aluminum cross beams and the three aluminum vertical beams has the advantages of simple structure, low cost, good stability and convenience in use. The upper clamping piece 121 and the lower clamping piece 122 can be two convex columns welded on the toothed bar 12, and because the through holes formed on the first and second aluminum beams 111 and 112 are slightly larger than the profile of the toothed bar 12, smooth up-and-down displacement of the toothed bar 12 can be ensured; when the rack bar 12 moves downwards, the upper clamping piece 121 abuts against the upper surface of the first aluminum beam 111, and the upper clamping piece limits the rack bar 12 to move downwards continuously; when the rack bar 12 moves upward until the lower clip piece 122 abuts against the lower surface of the first aluminum beam 111, the lower clip piece will limit the rack bar 12 to move upward, so as to ensure the rack bar 12 to move within a normal range.
Preferably, the upper and lower assemblies 15 and 14 are square plates, the profile of the upper assembly 15 is larger than that of the lower assembly 14, four circular holes are symmetrically formed in the lower assembly 14, T-shaped bolts 17 penetrate through the circular holes and are fixed on the lower plate surface of the upper assembly 15, and a pressure spring 18 is arranged between the T-shaped heads of the T-shaped bolts 17 and the lower plate surface of the lower assembly 14; a second stepper motor 16 is secured to the upper deck of the lower assembly 14. Through the cooperation of T type bolt 17 and round hole, can guarantee to go up subassembly 15 and move along upper and lower direction, can not take place the skew.
Referring to fig. 3, preferably, the stepping motor control module 40 includes a main control unit 41, an optoelectronic isolation unit 42, a stepping motor driving unit 43, and first and second power supplies 44 and 45; the main control unit 41 is used for receiving signals of the computing terminal 30 and sending out corresponding motor control signals, the photoelectric isolation unit 42 is used for isolating interference between the main control unit 41 and the stepping motor driving unit 43, the stepping motor driving unit 43 receives the isolated motor control signals and directly controls the steering and rotating angles of the first and second stepping motors 13 and 16, the first power supply 44 is used for supplying power to the input ends of the main control unit 41 and the photoelectric isolation unit 42, the second power supply 45 is used for supplying power to the output end of the photoelectric isolation unit 42 and the stepping motor driving unit 43, and the control and driving part adopts two independent first power supplies 44 and second power supplies 45, so that interference of the two power supplies can be isolated. The main control unit 41 comprises a main control chip, a clock circuit and a communication circuit, wherein the main control chip is an STM32 series chip, the main control chip exchanges data with the computing terminal 30 through the communication circuit, and the clock circuit is connected with the main control chip; the photoelectric isolation unit 42 is formed by connecting an optical coupler and a triode; the stepping motor driving unit 43 comprises an annular distributor, a subdivision circuit and a power amplifier, and the output ends of the annular distributor and the subdivision circuit are connected with the input end of the power amplifier; the computing terminal 30 is a computer, an industrial personal computer or a mobile terminal. The foregoing is a schematic block diagram of the stepper motor control module 40, and in practical application, a chip integrated with a plurality of units may be selected to implement the implementation, and fig. 4 shows a specific implementation scheme, where a glacier LC2054DA chip is directly used, the chip is a professional stepper motor driver, and the optical coupling isolation unit 42 is integrated in the chip, so that the use is very convenient, and the complexity of the circuit is reduced.
Referring to fig. 5, the present invention also discloses a detection method for the dynamic surface-enhanced raman spectroscopy detection apparatus as described above, including the following steps: a: the CCD sensor of the confocal Raman spectrometer 20 acquires an image of a measured sample and outputs the image to the computing terminal 30; b: the calculation terminal 30 gives a quantitative evaluation value J according to the definition of the image; c: the computing terminal 30 sends a control signal to the stepping motor control module 40, and the stepping motor control module 40 drives the motor in the dual-scale mobile detection platform 10 to act according to the control signal to adjust the position of the sample to be detected; repeating the step A and the step B, obtaining a new quantized evaluation value J of the definition, continuously adjusting the position of the sample to be measured according to the change of the quantized evaluation value J of the definition until the quantized evaluation value J of the definition is maximum, and finishing focusing; d: starting laser, and cutting off the laser after spectrum collection is completed; e: repeating the steps A to D until the detection of the single dynamic surface enhanced Raman spectrum is finished, for example, under the conventional test environment with the temperature of 20 ℃ and the humidity of 60%, the detection of the single dynamic surface enhanced Raman spectrum needs about 200s, the focusing of the liquid drop needs to be carried out once in about 20s, and the whole measurement needs 9 times of focusing. Through the steps, various defects of manual focusing can be well avoided, and a new way is provided for continuous and uninterrupted acquisition of the dynamic surface enhanced Raman spectrum.
Preferably, in the step B, the quantized evaluation value J ═ Σ of sharpnessM∑NL4(x, y) wherein L4The four-domain Laplacian is M, N pixel points of the image in the horizontal and vertical directions. The definition quantization evaluation value calculated by the formula is more referential, and the larger the value is, the better the definition is represented, and the better the definition is, the better the focusing effect is represented.
Preferably, the dual-scale mobile detection platform 10 comprises a base 11, a toothed bar 12 and a first stepping motor 13 fixed on the base 11, wherein the toothed bar 12 can move up and down along the base 11, and teeth of the toothed bar 12 are engaged with a toothed disc 131 on the first stepping motor 13; a lower component 14 is fixedly arranged at the top end of the rack bar 12, an upper component 15 is arranged above the lower component 14, a silicon wafer 50 containing a sample to be measured is placed on the upper component 15, a spring and a second stepping motor 16 are arranged between the upper component 15 and the lower component 14, the upper component 15 moves downwards under the action of the elastic force of the spring, a cam 161 is arranged on the shaft of the second stepping motor 16, and the space between the upper component 15 and the lower component 14 can be adjusted when the cam 161 rotates;
in the step C, the adjustment is carried out according to the following steps so that the quantized evaluation value J of the definition is maximum:
c1: controlling the first stepping motor 13 to move up and down, and obtaining the rotating direction of the first stepping motor 13 according to the change of the contrast definition; if J is increased when the first stepping motor 13 rotates forward, the rotation direction of the first stepping motor 13 is forward rotation, and the process proceeds to step C2; if J is increased when the first stepping motor 13 is rotated reversely, the rotation direction of the first stepping motor 13 is rotated reversely, and the process proceeds to step C2; if J becomes smaller when the first stepping motor 13 is rotated forward and J becomes smaller when the first stepping motor is rotated backward, the rotation of the first stepping motor 13 is stopped, and the process proceeds to step C3;
c2: after determining that the rotation direction of the first stepping motor 13 is forward rotation or reverse rotation, continuously rotating the first stepping motor 13 according to the rotation direction until the change trend of the definition is opposite, stopping rotating the first stepping motor 13, and entering step C3;
c3: and controlling the second stepping motor 16 according to the control strategy of the step C1 and the step C2, and finishing focusing when stopping rotating the second stepping motor 16.
In the above adjustment process, it is not necessary to adjust the quantized evaluation value J of sharpness to the maximum, and adjustment may not be continued as long as this value reaches the set threshold value, indicating that focusing has been completed. In addition, during the detection of single dynamic surface enhanced Raman spectrum, the adjustment is needed according to the steps C1-C3 for the first time; when focusing is performed again after the spectrum information is acquired once, the focus length is not changed much, and the first stepping motor 13 does not need to be adjusted, and only the second stepping motor 16 is adjusted, so that the focusing time can be greatly shortened.
Claims (9)
1. An apparatus for dynamic surface enhanced raman spectroscopy detection, characterized by: the device comprises a double-scale mobile detection platform (10), a confocal Raman spectrometer (20), a computing terminal (30) and a stepping motor control module (40), wherein the confocal Raman spectrometer (20) is positioned above the double-scale mobile detection platform (10) and acquires images and spectral information of a detected sample placed on the double-scale mobile detection platform (10), the computing terminal (30) receives the image information of the sample and outputs a control signal to the stepping motor control module (40) after analysis and processing, and the stepping motor control module (40) drives a motor in the double-scale mobile detection platform (10) to act so as to realize the focusing of the confocal Raman spectrometer (20) on the detected sample;
the double-scale movement detection platform (10) comprises a base (11), a toothed bar (12) and a first stepping motor (13) fixed on the base (11), wherein the toothed bar (12) can move up and down along the base (11), and teeth of the toothed bar (12) are meshed with a fluted disc (131) on the first stepping motor (13); the top of ratch (12) is fixed and is provided with down subassembly (14), the top of lower subassembly (14) is provided with subassembly (15), silicon chip (50) that hold the sample of being surveyed are placed on last subassembly (15), it is provided with spring and second step motor (16) to go up, lower subassembly (15, 14) within a definite time, go up subassembly (15) downstream under the spring force effect of spring, cam (161) and this cam (161) are installed to the epaxial adjustable upper and lower subassembly (15, 14) interval when rotating of second step motor (16).
2. The apparatus for dynamic surface-enhanced raman spectroscopy detection of claim 1 wherein: the rise distance of the cam (161) within 180 degrees, the tooth distance of the toothed rod (12) and the tooth distance of the upper toothed disc (131) of the first stepping motor (13) are equal to each other.
3. The apparatus for dynamic surface-enhanced raman spectroscopy detection of claim 1 wherein: the base (11) is formed by fixing a first, a second and a third aluminum cross beams (111, 112, 113) arranged from top to bottom and a first, a second and a third aluminum vertical beams (114, 115, 116) arranged from left to right through screws or bolts; the first and third aluminum cross beams (111, 113) and the first and third aluminum vertical beams (114, 116) are enclosed to form a square frame; the lower end of a second aluminum vertical beam (115) is fixed on a third aluminum cross beam (113), and the upper end of the second aluminum vertical beam (115) is fixedly provided with a first stepping motor (13); two ends of the second aluminum cross beam (112) are respectively fixed on the first and second aluminum vertical beams (114, 115), and through holes are respectively arranged on the first and second aluminum cross beams (111, 112) for the toothed bar (12) to pass through; an upper clamping piece (121) and a lower clamping piece (122) used for limiting are arranged on the rod body of the toothed rod (12), and the upper clamping piece (121) and the lower clamping piece (122) are respectively positioned on the upper side and the lower side of the through hole of the first aluminum cross beam (111).
4. The apparatus for dynamic surface-enhanced raman spectroscopy detection of claim 1 wherein: the upper assembly (15) and the lower assembly (14) are both square plates, the outline of the upper assembly (15) is larger than that of the lower assembly (14), four round holes are symmetrically formed in the lower assembly (14), T-shaped bolts (17) penetrate through the round holes and are fixed on the lower plate surface of the upper assembly (15), and pressure springs (18) are arranged between T-shaped heads of the T-shaped bolts (17) and the lower plate surface of the lower assembly (14); the second stepping motor (16) is fixed on the upper plate surface of the lower component (14).
5. The apparatus for dynamic surface-enhanced raman spectroscopy of claim 1 or 2 wherein: the stepping motor control module (40) comprises a main control unit (41), a photoelectric isolation unit (42), a stepping motor driving unit (43) and a first power supply (44) and a second power supply (45); the main control unit (41) is used for receiving signals of the computing terminal (30) and sending corresponding motor control signals, the photoelectric isolation unit (42) is used for isolating interference between the main control unit (41) and the stepping motor driving unit (43), the stepping motor driving unit (43) receives the isolated motor control signals and directly controls the steering and rotating angles of the first stepping motor (13) and the second stepping motor (16), the first power supply (44) is used for supplying power to the main control unit (41) and the input end of the photoelectric isolation unit (42), and the second power supply (45) is used for supplying power to the output end of the photoelectric isolation unit (42) and the stepping motor driving unit (43).
6. The apparatus for dynamic surface-enhanced raman spectroscopy of claim 5 wherein: the main control unit (41) comprises a main control chip, a clock circuit and a communication circuit, wherein the main control chip is an STM32 series chip, the main control chip exchanges data with the computing terminal (30) through the communication circuit, and the clock circuit is connected with the main control chip; the photoelectric isolation unit (42) is formed by connecting an optical coupler and a triode; the stepping motor driving unit (43) comprises an annular distributor, a subdivision circuit and a power amplifier, and the output ends of the annular distributor and the subdivision circuit are connected with the input end of the power amplifier; the computing terminal (30) is a computer or a mobile terminal.
7. A method of detecting the apparatus for dynamic surface enhanced raman spectroscopy detection of claim 1 comprising the steps of:
a: a CCD sensor of the confocal Raman spectrometer (20) acquires an image of a measured sample and outputs the image to a computing terminal (30);
b: the computing terminal (30) gives a quantitative evaluation value J according to the definition of the image;
c: the computing terminal (30) sends a control signal to the stepping motor control module (40), and the stepping motor control module (40) drives a motor in the dual-scale mobile detection platform (10) to act according to the control signal to adjust the position of the sample to be detected; repeating the step A and the step B, obtaining a new quantized evaluation value J of the definition, continuously adjusting the position of the sample to be measured according to the change of the quantized evaluation value J of the definition until the quantized evaluation value J of the definition is maximum, and finishing focusing;
d: starting laser, and cutting off the laser after spectrum collection is completed;
e: and repeating the steps A to D until the detection of the single dynamic surface enhanced Raman spectrum is finished.
8. The detection method of an apparatus for dynamic surface-enhanced raman spectroscopy according to claim 7 wherein: saidIn step B, the quantized evaluation value J of sharpness is ΣM∑NL4(x, y) wherein L4The four-domain Laplacian is used as an operator, M, N are pixels of the image in the horizontal direction and the vertical direction, and x and y are gray values of the pixels in the horizontal direction and the vertical direction.
9. The detection method of an apparatus for dynamic surface-enhanced raman spectroscopy according to claim 7 wherein: in the step C, the adjustment is carried out according to the following steps so that the quantized evaluation value J of the definition is maximum:
c1: controlling the first stepping motor (13) to move up and down, and obtaining the rotating direction of the first stepping motor (13) according to the change of the contrast definition;
if J is increased when the first stepping motor (13) rotates forwards, the rotating direction of the first stepping motor (13) rotates forwards, and the step C2 is executed;
if J is increased when the first stepping motor (13) rotates reversely, the rotation direction of the first stepping motor (13) is reversed, and the process proceeds to step C2;
if J becomes smaller when the first stepping motor (13) rotates forward and J becomes smaller when the first stepping motor rotates backward, stopping rotating the first stepping motor (13), and proceeding to step C3;
c2: after the rotation direction of the first stepping motor (13) is determined to be forward rotation or reverse rotation, the first stepping motor (13) is continuously rotated according to the rotation direction until the change trend of the definition is opposite, the first stepping motor (13) is stopped to rotate, and the step C3 is carried out;
c3: and C1 and C2, controlling the second stepping motor (16) to finish focusing when the second stepping motor (16) stops rotating.
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| CN112683880B (en) * | 2020-12-28 | 2022-06-07 | 山东大学 | Device and method for rapidly determining mineral content based on Raman spectrum analysis |
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| CN1580746A (en) * | 2003-08-06 | 2005-02-16 | 北京航空航天大学 | Matrix type biochip CCD scanning fetch device |
| CN2662236Y (en) * | 2003-12-23 | 2004-12-08 | 广东省农业科学院作物研究所 | A peanut simple grain diffuse reflection spectrum measurement device |
| JP2005189634A (en) * | 2003-12-26 | 2005-07-14 | Nikon Corp | Focus detector for camera |
| JP5142315B2 (en) * | 2007-07-18 | 2013-02-13 | 独立行政法人情報通信研究機構 | Microscope and observation method thereof |
| CN103399398B (en) * | 2013-07-30 | 2015-07-15 | 济南华天恒达科技有限公司 | Automatically-focused microscope |
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| US20150205088A1 (en) * | 2014-01-23 | 2015-07-23 | U&U Engineering Inc. | Bevel-axial auto-focusing microscopic system and method thereof |
| CN103792686B (en) * | 2014-01-27 | 2015-09-16 | 欧普康视科技股份有限公司 | A kind of contact lens comprehensive detector |
| CN104133288B (en) * | 2014-07-11 | 2017-07-28 | 广州博隆兴中信息科技有限公司 | A kind of continuous zoom auto-focusing microscopic imaging device and method towards biological tissue |
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