CN106967664A - A kind of preparation method for the nutriment for promoting microorganism to grow - Google Patents
A kind of preparation method for the nutriment for promoting microorganism to grow Download PDFInfo
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- CN106967664A CN106967664A CN201710308487.3A CN201710308487A CN106967664A CN 106967664 A CN106967664 A CN 106967664A CN 201710308487 A CN201710308487 A CN 201710308487A CN 106967664 A CN106967664 A CN 106967664A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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Abstract
The invention discloses a kind of preparation method for the nutriment for promoting microorganism to grow, it is related to microbial technology field.Methods described includes：By homeothermal animal blood and distilled water according to 1：The volume ratio mixing of (10~20), obtains mixed liquor；The mixed liquor is sufficiently stirred for using mixer, material liquid is obtained；The material liquid is placed in reactor, reactor is placed in 190 DEG C~200 DEG C, under conditions of more than or equal to 2 atmospheric pressures, the material liquid in reactor is hydrolyzed, after hydrolyzate is filtered, obtain filtrate, the filtrate is the nutriment for promoting microorganism growth, completes to promote the preparation of the nutriment of microorganism growth.The nutriment that the present invention is prepared improves production efficiency, reduces cost, also improves the efficiency of clinical detection speed, reduces culture medium pollution, Extending culture base holding time.
Description
Technical field
The present invention relates to microbial technology field, more particularly to a kind of preparation side for the nutriment for promoting microorganism to grow
Method.
Background technology
Microorganism is the tiny organism that a kind of naked eyes can not be observed, including：Bacterium, fungi, protist, microalga
And virus.Microorganism individual is small, species is various, close with human relation.Microorganism includes harmful microorganism and beneficial micro- life
Thing, relates generally to the numerous areas such as food, medicine, industrial or agricultural, environmental protection.
In microbial cultivation process, in order to be supplied to the nutrition that microorganism is enough, promote microorganism growth, commonly using dynamic
Thing blood or serum, such as Colombia's blood plate, chocolate flat board.But, the problem of existing is：Add blood or serum
When, for the active material loss of activity in anti-Hemostatic Oral Liquid or serum, basal medium temperature when addition blood or serum is not
It is preferably too high, autoclave sterilization can not be also carried out, meanwhile, and because with the addition of blood or the culture medium effective time of serum is short,
Therefore, the culture medium of existing addition blood or serum can influence to be cultured the utilization of microorganism and the micro- life of pathogeny in use
The diagnosis of thing.
The content of the invention
It is an object of the invention to provide a kind of preparation method for the nutriment for promoting microorganism to grow, so as to solve existing
There are foregoing problems present in technology.
To achieve these goals, the preparation method of the nutriment of the present invention for promoting microorganism to grow, the side
Method includes：
S1, by homeothermal animal blood and distilled water according to 1：The volume ratio mixing of (10~20), obtains mixed liquor；
S2, is sufficiently stirred for the mixed liquor using mixer, obtains material liquid；
S3, the material liquid is placed in reactor, in 190 DEG C~200 DEG C, the condition more than or equal to 2 atmospheric pressures
Under, the material liquid in the reactor is hydrolyzed, after hydrolyzate is filtered, filtrate is obtained, the filtrate is to promote
Enter the nutriment of microorganism growth, complete to promote the preparation of the nutriment of microorganism growth.
Preferably, the homeothermal animal blood is warm-blooded mammalian blood or constant temperature avian blood.
Preferably, the filtrate is added in solid medium or fluid nutrient medium.
Preferably, the filtrate and the solid medium or with the volume ratio relation of the fluid nutrient medium for (1~
10)：100.
Preferably, in step S3, the hydrolysis time that the material liquid in reactor is hydrolyzed is 1h~2h.
The beneficial effects of the invention are as follows：
(1) nutriment that the present invention is obtained promotes the growth of industrial microbe, improves production efficiency, reduces cost.
(2) nutriment that the present invention is obtained promotes invasive organism growth, improves the efficiency of clinical detection speed.
(3) the nutriment alternative serum and blood that the present invention is obtained are added in culture medium, while high temperature can be carried out
Autoclaving, reduces culture medium pollution, Extending culture base holding time.
Brief description of the drawings
Fig. 1 is the schematic flow sheet of the preparation method for the nutriment for promoting microorganism growth.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, below in conjunction with accompanying drawing, the present invention is entered
Row is further described.It should be appreciated that embodiment described herein is not used to only to explain the present invention
Limit the present invention.
Embodiment
Promote the preparation method of the nutriment of microorganism growth described in the present embodiment, methods described includes：
S1, by homeothermal animal blood and distilled water according to 1：The volume ratio mixing of (10~20), obtains mixed liquor；
S2, is sufficiently stirred for the mixed liquor using mixer, obtains material liquid；
S3, the material liquid is placed in reactor, and reactor is placed in into 190 DEG C~200 DEG C, more than or equal to 2 air
Under conditions of pressure, the material liquid in reactor is hydrolyzed, after hydrolyzate is filtered, filtrate, the filtrate is obtained
As promote the nutriment of microorganism growth, complete to promote the preparation of the nutriment of microorganism growth.
Explanation, which is explained in more detail, is：The homeothermal animal blood be warm-blooded mammalian blood or constant temperature avian blood,
Homeothermal animal blood constituent is close, and product after treatment is similar.The filtrate is added to solid medium or Liquid Culture
In base.The filtrate and the solid medium are (1~10) with the volume ratio relation of the fluid nutrient medium：100.Step
In rapid S3, the hydrolysis time that the material liquid in reactor is hydrolyzed is 1h~2h.It is described to promote what microorganism grew
Nutriment can promote bacterium, fungi growth；Various severe bacteria growths are may additionally facilitate, raising, which is clinically separated, to cultivate one's ability.Micro-
After the nutriment that the promotion microorganism growth is added in biological medium, microbiological culture media, which has, shortens microculture
Time, improve microculture efficiency, be readily available the features such as raw material, manufacturing process are easy.
Embodiment 1 promotes the preparation of the nutriment of microorganism growth
A1, animal blood pre-treatment：Pig blood 1000ml is purchased in the market of farm produce, takes 100ml to add 9000ml distilled water, obtains
To pig blood mixed liquor, pig blood mixed liquor is stirred using the broken wall cooking machines of SKG 1246 and smashes sludged blood, obtain pig blood former
Feed liquid, rotating speed is 1200 turns/min, and the time is 3 minutes；
A2, high temperature and high pressure hydrolysis：Pig blood material liquid 1000ml after stir process of learning from else's experience, is put into HTHP reflection
Kettle, under conditions of 195 DEG C of temperature, 2.1 atmospheric pressure of atmospheric pressure, speed of agitator is 100 revs/min, is carried out high while stirring
Warm highly pressured hydrolysis reaction, the reaction time is 1h, obtains hydrolyzate；The reactor is that Weihai Chemical Machinery Co., Ltd. GSH is high
Warm high pressure reflects kettle；
A3, using 10 layers of sterile gauze hydrolyzate is filtered, it is the battalion for promoting microorganism growth to obtain filtered fluid
Support material, atmospheric pressure, 20 degree it is lower carry out dynamic viscosities and kinematic viscosity is tested, obtain for：Action edge viscosity, mu=1.19 ×
10-3Pas, kinematic viscosity v=1.20 × 10-6 ㎡/s；Carried out using Hangzhou Zhuo Xiang Science and Technology Ltd.s VSM automatic viscometers
Dynamic viscosity and kinematic viscosity test；
A4, room temperature after filtered fluid autoclave sterilization saved backup；The condition of autoclave sterilization is 121 DEG C, air
Press 1.2 atmospheric pressure, sterilization time 15min.
Embodiment 2：Addition promotes the nutrient agar of the nutriment of microorganism growth and is not added with promoting micro- life
The nutrient agar contrast test of the nutriment of thing growth
1st, the nutrient agar panel for the nutriment for being not added with promoting microorganism growth is prepared
Peptone 10.0g, powdered beef 3.0g, sodium chloride 5.0g, agar 15.0g are weighed, distilled water 1L is dissolved in, pH value is adjusted
Sterilized 15 minutes under 7.3 (25 DEG C), 121 degree of 1.2 atmospheric pressure, cast prepares nutrient agar, as a control group.
2nd, the nutrient agar panel that addition promotes the nutriment of microorganism growth is prepared
Weigh peptone 10.0g, powdered beef 3.0g, sodium chloride 5.0g, agar 15.0g, promotion prepared by addition embodiment 1
The nutriment 100ml of microorganism growth, is dissolved in after distilled water 900ml, autoclaving, cast prepares plating medium as examination
Test group.
3rd, following bacteriums are inoculated with two kinds of platen parallels, staphylococcus aureus, MRSE, Escherichia coli, gram
Thunder wins bacterium, and every kind of three flat boards of microbionation are observed by culture, it is found that the test group formation bacterium colony time is faster than control group,
Bacterium colony after cultivating 24 hours is bigger than control group, refers to table 1.
Table 1 adds and is not added with to promote the microculture test of the nutrient agar panel of the nutriment of microorganism growth
As a result
Obtained by table 1, the growth of bacterium can be promoted by promoting the nutriment of microorganism growth.
Embodiment 3：Addition promotes the salt czapek dox agar culture medium of the nutriment of microorganism growth and is not added with promoting
The salt czapek dox agar culture medium contrast test of the nutriment of microorganism growth
1st, the salt czapek dox agar flat board for the nutriment for being not added with promoting microorganism growth is prepared
The salt czapek dox agar 109g purchased from Qingdao Hai Bo bio tech ltd is weighed, 1L distilled water, high pressure is dissolved in
Flat board cooling is poured into after sterilizing, as a control group.
2nd, the salt czapek dox agar flat board that addition promotes the nutriment of microorganism growth is prepared
The salt czapek dox agar 109g purchased from Qingdao Hai Bo bio tech ltd is weighed, 100mL is added and promotes micro- life
The nutriment and 900mL distilled water of thing growth, are mixed, and dispensing after autoclaving is tested group.
3rd, following fungi is inoculated with two kinds of platen parallels, aspergillus niger, aspergillus fumigatus, Candida albicans, Candida tropicalis are every kind of
Fungi three flat boards of inoculation, are observed by culture, it is found that the test group formation bacterium colony time is faster than control group, after cultivating 24 hours
Bacterium colony it is bigger than control group, refer to table 2.
Table 2 adds and is not added with to promote the microculture of the salt czapek dox agar flat board of the nutriment of microorganism growth
Test result
Obtained by table 2, the growth of fungi can be promoted by promoting the nutriment of microorganism growth.
Embodiment 4：Addition promotes the nutrient agar and Colombia's blood plate of the nutriment of microorganism growth
The severe bacteria Contrast on effect test of medium culture
1st, Colombia's blood plate of Qingdao Hai Bo bio tech ltd, as a control group 1 are bought.
2nd, the nutrient agar panel of the nutriment of microorganism growth is promoted to compare group 2 using the addition in embodiment 1.
3rd, the nutrient agar panel of the nutriment of microorganism growth is promoted to be tested group using the addition in embodiment 1.
4th, in three kinds of platen parallel inoculation streptococcus pneumonias, haemophilus influenzaes, every kind of three flat boards of inoculation, in CO2 trainings
Support case to be cultivated, it is found that test group and control group 1 can cultivate severe bacteria, control group 2 has no bacterial growth, refers to table 3.
The microorganism testing result of the embodiment 3 of table 3
Embodiment 5：Shelf-life tests
The 1st, addition not after tested prepared in Examples 1 and 2 is promoted to the blank of the nutriment of microorganism growth
Flat board stores one week, 1 month in 4 DEG C of refrigerators and takes out culture medium flat plate after 3 months, is used as test group.
2nd, Examples 1 and 2,3 experiment are repeated in using after storing one week, 1 month and 3 months.
3rd, test result is shown, indifference when there is bacterium colony time and bacterium colony size with just preparing, and illustrates present invention culture
Base shelf-stable, retention is good.
Because pig blood is easily started with, pig blood is used in this embodiment 1-5, it is contemplated that homeothermal animal blood constituent
Close, component difference is little, while it is small to consider that homeothermal animal blood constituent is hydrolyzed to by macromolecular after high temperature and high pressure hydrolysis
Composition after fragment is more close, and more amino acids, monose and lower fatty acid are contained after hydrolysis, can deduce various dynamic
The composition of thing blood after treatment is more or less the same, and amino acid and monose and lower fatty acid are easier by bacterium intake and
Utilize, bacterial metabolism mechanism can save the energy needed for albumen and many glycometabolisms, focus on growth division, promote bacterium life
It is long.
By using above-mentioned technical proposal disclosed by the invention, following beneficial effect has been obtained：
(1) nutriment that the present invention is obtained promotes the growth of industrial microbe, improves production efficiency, reduces cost.
(2) nutriment that the present invention is obtained promotes invasive organism growth, improves the efficiency of clinical detection speed.
(3) the nutriment alternative serum and blood that the present invention is obtained are added in culture medium, while high temperature can be carried out
Autoclaving, reduces culture medium pollution, Extending culture base holding time.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
Depending on protection scope of the present invention.
Claims (5)
1. a kind of preparation method for the nutriment for promoting microorganism to grow, it is characterised in that methods described includes：
S1, by homeothermal animal blood and distilled water according to 1：The volume ratio mixing of (10~20), obtains mixed liquor；
S2, is sufficiently stirred for the mixed liquor using mixer, obtains material liquid；
S3, the material liquid is placed in reactor, and reactor is placed in into 190 DEG C~200 DEG C, more than or equal to 2 atmospheric pressures
Under conditions of, the material liquid in reactor is hydrolyzed, after hydrolyzate is filtered, filtrate is obtained, the filtrate is
Promote the nutriment of microorganism growth, complete to promote the preparation of the nutriment of microorganism growth.
2. promote the preparation method of the nutriment of microorganism growth according to claim 1, it is characterised in that the constant temperature
Animal blood is warm-blooded mammalian blood or constant temperature avian blood.
3. promote the preparation method of the nutriment of microorganism growth according to claim 1, it is characterised in that the filtrate
It is added in solid medium or fluid nutrient medium.
4. promote the preparation method of the nutriment of microorganism growth according to claim 3, it is characterised in that the filtrate
It is (1~10) with the solid medium or with the volume ratio relation of the fluid nutrient medium：100.
5. promote the preparation method of the nutriment of microorganism growth according to claim 1, it is characterised in that step S3
In, the hydrolysis time that the material liquid in reactor is hydrolyzed is 1h~2h.
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| CN201710308487.3A CN106967664A (en) | 2017-05-04 | 2017-05-04 | A kind of preparation method for the nutriment for promoting microorganism to grow |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59159772A (en) * | 1983-03-04 | 1984-09-10 | Toyo Soda Mfg Co Ltd | Preparation of mold of microorganism |
| CN1283397A (en) * | 2000-07-20 | 2001-02-14 | 上海交通大学 | Process for preparing decoloured protono by pressure hydrolysis of animal blood |
| CN101717281A (en) * | 2009-12-11 | 2010-06-02 | 湖北大学 | Method for preparing edible fungi growth promoter by protein-containing waste |
-
2017
- 2017-05-04 CN CN201710308487.3A patent/CN106967664A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59159772A (en) * | 1983-03-04 | 1984-09-10 | Toyo Soda Mfg Co Ltd | Preparation of mold of microorganism |
| CN1283397A (en) * | 2000-07-20 | 2001-02-14 | 上海交通大学 | Process for preparing decoloured protono by pressure hydrolysis of animal blood |
| CN101717281A (en) * | 2009-12-11 | 2010-06-02 | 湖北大学 | Method for preparing edible fungi growth promoter by protein-containing waste |
Non-Patent Citations (1)
| Title |
|---|
| 赵红艳等: ""鲜猪血加压水解制备复合氨基酸新工艺的研究"", 《氨基酸和生物资源》 * |
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Application publication date: 20170721 |
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