CN106947730A - A kind of culture plant cell method for producing high content mineral matter element and its application - Google Patents
A kind of culture plant cell method for producing high content mineral matter element and its application Download PDFInfo
- Publication number
- CN106947730A CN106947730A CN201710290484.1A CN201710290484A CN106947730A CN 106947730 A CN106947730 A CN 106947730A CN 201710290484 A CN201710290484 A CN 201710290484A CN 106947730 A CN106947730 A CN 106947730A
- Authority
- CN
- China
- Prior art keywords
- plant cell
- culture
- cell
- mineral matter
- mineral
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229910052500 inorganic mineral Inorganic materials 0.000 title claims abstract description 49
- 239000011707 mineral Substances 0.000 title claims abstract description 49
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 8
- 241000196324 Embryophyta Species 0.000 claims abstract description 84
- 241000219095 Vitis Species 0.000 claims abstract description 31
- 239000001963 growth medium Substances 0.000 claims abstract description 29
- 235000009392 Vitis Nutrition 0.000 claims abstract description 27
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000011575 calcium Substances 0.000 claims abstract description 18
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 18
- 229910052742 iron Inorganic materials 0.000 claims abstract description 16
- 239000011777 magnesium Substances 0.000 claims abstract description 16
- 229910052749 magnesium Inorganic materials 0.000 claims abstract description 16
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000011701 zinc Substances 0.000 claims abstract description 11
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 11
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 10
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000004113 cell culture Methods 0.000 claims abstract description 6
- 235000013305 food Nutrition 0.000 claims abstract description 5
- 230000010261 cell growth Effects 0.000 claims abstract description 4
- 239000002366 mineral element Substances 0.000 claims abstract description 4
- 239000006143 cell culture medium Substances 0.000 claims abstract description 3
- 239000007787 solid Substances 0.000 claims description 38
- 239000000843 powder Substances 0.000 claims description 27
- 239000002609 medium Substances 0.000 claims description 26
- 239000007788 liquid Substances 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 12
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 9
- 235000015097 nutrients Nutrition 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 6
- 150000002500 ions Chemical class 0.000 claims description 6
- 238000009630 liquid culture Methods 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 239000002775 capsule Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 5
- 239000003826 tablet Substances 0.000 claims description 5
- 235000013402 health food Nutrition 0.000 claims description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 3
- 235000013361 beverage Nutrition 0.000 claims description 3
- 229910017052 cobalt Inorganic materials 0.000 claims description 3
- 239000010941 cobalt Substances 0.000 claims description 3
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims description 3
- 235000009508 confectionery Nutrition 0.000 claims description 3
- 229910052802 copper Inorganic materials 0.000 claims description 3
- 239000010949 copper Substances 0.000 claims description 3
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 238000012549 training Methods 0.000 claims description 3
- 240000005373 Panax quinquefolius Species 0.000 claims description 2
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 2
- 241001373816 Saussurea laniceps Species 0.000 claims description 2
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims description 2
- 239000002738 chelating agent Substances 0.000 claims description 2
- 235000013365 dairy product Nutrition 0.000 claims description 2
- 239000007919 dispersible tablet Substances 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 239000005556 hormone Substances 0.000 claims description 2
- 229940088597 hormone Drugs 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims description 2
- 229910052640 jadeite Inorganic materials 0.000 claims description 2
- 229910052711 selenium Inorganic materials 0.000 claims description 2
- 239000011669 selenium Substances 0.000 claims description 2
- 235000015170 shellfish Nutrition 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims 1
- 230000000763 evoking effect Effects 0.000 claims 1
- 239000000499 gel Substances 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 239000006187 pill Substances 0.000 claims 1
- 230000036541 health Effects 0.000 abstract description 6
- 238000010521 absorption reaction Methods 0.000 abstract description 5
- 230000000035 biogenic effect Effects 0.000 abstract description 2
- 229930000044 secondary metabolite Natural products 0.000 abstract 1
- 230000000638 stimulation Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 61
- 235000010755 mineral Nutrition 0.000 description 32
- 229910052564 epsomite Inorganic materials 0.000 description 16
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 16
- 229930006000 Sucrose Natural products 0.000 description 14
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 14
- 239000005720 sucrose Substances 0.000 description 14
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 9
- 239000001110 calcium chloride Substances 0.000 description 9
- 229910001628 calcium chloride Inorganic materials 0.000 description 9
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 8
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 8
- 229960005069 calcium Drugs 0.000 description 8
- 229910001424 calcium ion Inorganic materials 0.000 description 8
- 229910001425 magnesium ion Inorganic materials 0.000 description 8
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 235000021552 granulated sugar Nutrition 0.000 description 6
- 235000013336 milk Nutrition 0.000 description 6
- 239000008267 milk Substances 0.000 description 6
- 210000004080 milk Anatomy 0.000 description 6
- 230000006698 induction Effects 0.000 description 5
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 5
- 229910000368 zinc sulfate Inorganic materials 0.000 description 5
- WAEMQWOKJMHJLA-UHFFFAOYSA-N Manganese(2+) Chemical compound [Mn+2] WAEMQWOKJMHJLA-UHFFFAOYSA-N 0.000 description 4
- 240000000111 Saccharum officinarum Species 0.000 description 4
- 235000007201 Saccharum officinarum Nutrition 0.000 description 4
- 235000009754 Vitis X bourquina Nutrition 0.000 description 4
- 235000012333 Vitis X labruscana Nutrition 0.000 description 4
- 235000014787 Vitis vinifera Nutrition 0.000 description 4
- PGTXKIZLOWULDJ-UHFFFAOYSA-N [Mg].[Zn] Chemical compound [Mg].[Zn] PGTXKIZLOWULDJ-UHFFFAOYSA-N 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- ZFXVRMSLJDYJCH-UHFFFAOYSA-N calcium magnesium Chemical compound [Mg].[Ca] ZFXVRMSLJDYJCH-UHFFFAOYSA-N 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 235000013601 eggs Nutrition 0.000 description 4
- 229910001437 manganese ion Inorganic materials 0.000 description 4
- 229910052603 melanterite Inorganic materials 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 239000011686 zinc sulphate Substances 0.000 description 4
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 241001145025 Saussurea involucrata Species 0.000 description 3
- 229930014669 anthocyanidin Natural products 0.000 description 3
- 150000001452 anthocyanidin derivatives Chemical class 0.000 description 3
- 235000008758 anthocyanidins Nutrition 0.000 description 3
- 235000014103 egg white Nutrition 0.000 description 3
- 210000000969 egg white Anatomy 0.000 description 3
- 235000013312 flour Nutrition 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- QJVXKWHHAMZTBY-GCPOEHJPSA-N syringin Chemical compound COC1=CC(\C=C\CO)=CC(OC)=C1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 QJVXKWHHAMZTBY-GCPOEHJPSA-N 0.000 description 3
- QJVXKWHHAMZTBY-KSXIZUIISA-N syringin Natural products COc1cc(C=CCO)cc(OC)c1O[C@H]2O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2O QJVXKWHHAMZTBY-KSXIZUIISA-N 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 2
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 235000014121 butter Nutrition 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 210000002969 egg yolk Anatomy 0.000 description 2
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000004114 suspension culture Methods 0.000 description 2
- DCXXMTOCNZCJGO-UHFFFAOYSA-N tristearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCCCCCCCCCC)COC(=O)CCCCCCCCCCCCCCCCC DCXXMTOCNZCJGO-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- WBZFUFAFFUEMEI-UHFFFAOYSA-M Acesulfame k Chemical compound [K+].CC1=CC(=O)[N-]S(=O)(=O)O1 WBZFUFAFFUEMEI-UHFFFAOYSA-M 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 229920000168 Microcrystalline cellulose Polymers 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 241000194020 Streptococcus thermophilus Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 229930003316 Vitamin D Natural products 0.000 description 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 229960004998 acesulfame potassium Drugs 0.000 description 1
- 235000010358 acesulfame potassium Nutrition 0.000 description 1
- 239000000619 acesulfame-K Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000012970 cakes Nutrition 0.000 description 1
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 description 1
- 239000001527 calcium lactate Substances 0.000 description 1
- 229960002401 calcium lactate Drugs 0.000 description 1
- 235000011086 calcium lactate Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 235000019219 chocolate Nutrition 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 239000011651 chromium Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000006196 drop Substances 0.000 description 1
- 238000007908 dry granulation Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 230000008216 juvenile development Effects 0.000 description 1
- 229910001655 manganese mineral Inorganic materials 0.000 description 1
- WJZHMLNIAZSFDO-UHFFFAOYSA-N manganese zinc Chemical compound [Mn].[Zn] WJZHMLNIAZSFDO-UHFFFAOYSA-N 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 235000019813 microcrystalline cellulose Nutrition 0.000 description 1
- 239000008108 microcrystalline cellulose Substances 0.000 description 1
- 229940016286 microcrystalline cellulose Drugs 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- 239000006870 ms-medium Substances 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229910052718 tin Inorganic materials 0.000 description 1
- 239000011135 tin Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 229910052720 vanadium Inorganic materials 0.000 description 1
- LEONUFNNVUYDNQ-UHFFFAOYSA-N vanadium atom Chemical compound [V] LEONUFNNVUYDNQ-UHFFFAOYSA-N 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
- A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
- A21D2/36—Vegetable material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1307—Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1322—Inorganic compounds; Minerals, including organic salts thereof, oligo-elements; Amino-acids, peptides, protein-hydrolysates or derivatives; Nucleic acids or derivatives; Yeast extract or autolysate; Vitamins; Antibiotics; Bacteriocins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/364—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/42—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds characterised by the carbohydrates used, e.g. polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/50—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by shape, structure or physical form, e.g. products with supported structure
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
- C12N2500/14—Calcium; Ca chelators; Calcitonin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
- C12N2500/16—Magnesium; Mg chelators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/22—Zinc; Zn chelators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Nutrition Science (AREA)
- Inorganic Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Botany (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of culture plant cell method for producing high content mineral matter element and its application.It is the concentration of the ion of the corresponding mineral matter element in raising culture medium during culture plant cell, and the cell culture rich in organic biogenic mineral prime element is converted into by plant cell growth, absorption, enrichment.Simultaneously because of the raising and then the production of the active secondary metabolite of stimulation plant cell of corresponding biological mineral matter content, so as to further improve the function and application value of cell culture.In the present invention, by improving the mineral element such as calcium, magnesium, iron, zinc in Vitis and Herba Saussureae Involueratae cell culture medium, the Mineral element content in Vitis and Herba Saussureae Involueratae cell can correspondingly be improved, and then the food of correlation, field of health care products are may apply to, open up the new application of the downstream industry product of corresponding plant cell.
Description
The application obtains the subsidy of Tianjin key lab of Medicinal Plant Cell scale evaluation enterprise.
Technical field
The invention belongs to technical field of biological fermentation, it is related to a kind of plant cell rich in biogenic mineral prime element nutrition former
The production method of material and its food and health products, particularly a kind of culture plant cell method for producing high content mineral matter element
And its application.
Background technology
Mineral matter is the general name for constituting tissue and maintaining various elements necessary to normal physiological function, is needed by human
One of seven major nutrient.The various elements contained in human body, exist except carbon, oxygen, hydrogen, nitrogen etc. are main in the form of organic matter
In addition, remaining 60 multiple element is referred to as mineral matter (being also inorganic salts).Wherein 25 kinds required for human nutrition.Calcium,
Magnesium, potassium, sodium, phosphorus, sulphur, 7 kinds of constituent contents of chlorine are more, account for the 60% ~ 80% of mineral matter total amount, referred to as macroelement.Other yuan
, there is quantity seldom in element such as totally 14 kinds of iron, copper, iodine, zinc, manganese, molybdenum, cobalt, chromium, tin, vanadium, silicon, nickel, fluorine, few in machine in-vivo content
In 0. 005%, it is referred to as trace element.
Mineral matter constitutes or maintained normal physiological effect to play important function in tissue, and mineral matter be unable to itself
Synthesis, it is necessary to supplied by external environment.With the difference of age, sex, health, environment and working condition, to mineral matter
Demand be different.Interim, the basic diet when Juvenile development phase, women gestation phase, old man's climacteric etc. are specific
It is the demand that can not meet body to mineral matter, it is therefore desirable to extra mineral matter element intake.
With the development of society, the health perception of people gradually strengthens, and market more becomes more meticulous for the demand of mineral matter.
Culture plant cell is the totipotency using plant cell, and can be obtained by regulation culture CMC model has multiple biological activities
The plant cell powder of composition and mineral matter element, makes it be applied in the field of related industry product, such as food or health care
Product field, has very important perspective influence in big health industry.
The content of the invention
The invention discloses a kind of culture plant cell method for producing high content mineral matter element, it is characterised in that by such as
Under step carry out：
（1）Plant cell callus is induced：Include root, stem and leaf flowers fruit etc. from the various explants of plant, plant skin or seed
The explants such as aseptic seedling, young leaves, stem, the root of sprouting are set out, and the ratio induction of hormone needed for by adjusting plant cell growth is cured
Injured tissue is produced.
（2）Plant cell squamous subculture
Plant cell solid medium squamous subculture：Plant cell chooses some solids after solid medium culture 10-40 days
The preferable plant cell of character is inoculated into new preparation solid medium in culture medium, that is, completes solid medium inoculation；
Plant cell liquid culture medium squamous subculture：The preferable plant cell of state in solid medium is transferred to Liquid Culture
Base culture, in liquid fresh plant cell culture medium culture 7-30 days, the seed cell after culture is passed through in Biohazard Safety Equipment
80 mesh sieve net filtrations, the 5.0-10.0 g wet cells after filtering are inoculated with 100ml/500ml shaking flasks, that is, complete plant cell
The squamous subculture of fluid nutrient medium；
（3）The addition of culture medium Mineral Elements in Jadeite Shellfish
The addition of solid plant cell culture mineral matter element：
In the preparation of solid plant Subculture culture medium, on the basis of original culture medium, characteristic is carried according to demand
5-100 times of the ion concentration of certain in high solid culture medium or multi mineral prime element, carries out solid plant Subculture；
The addition of liquid plant cell culture mineral matter element：
, can be on the basis of original culture medium in liquid plant Cell Subculture, characteristic improves solid according to demand
5-100 times of the ion concentration of certain in body culture medium or multi mineral prime element, carries out liquid plant Subculture；Also may be used
With solion that is disposable during culture plant cell or adding certain or multi mineral prime element in multiple times, add again
Number is 5-100 times, continues culture plant cell；
（4）Culture plant cell
Solid plant cell is placed in culture plant cell case and cultivated, and collects cell after 10-40 days, processing is dried, obtain dry
Dry plant cell powder, afterwards using the content of mineral substances in aas determination plant cell；
Liquid Culture plant cell is placed in shaken cultivation or culture, 15-30 in suitable bioreactor on 100 rpm shaking tables
DEG C culture 7-30 days after harvest, processing is dried, dried plant cell powder is obtained, afterwards using aas determination plant
Content of mineral substances in cell；
（5）Using plant cell dried powder, add related auxiliary material and prepare relevant food or health food.It is of the present invention
Plant cell includes：Herba Saussureae Involueratae cell, Vitis, but be not limited to both cells, also including but not limited to ginseng-cell,
Apple cells, Saussurea laniceps cell, American Ginseng cell.Described mineral matter element includes：Calcium, magnesium, iron, zinc, but it is not limited to this
Four kinds of mineral matter elements, including copper, manganese, selenium, cobalt or other metal-chelator forms.
The present invention further discloses using the method produce high content mineral matter element plant cell dry powder with
Application in terms of cake, candy, dairy products, beverage or fermented wine is made.And for tablet, capsule, granule, drop
Application in terms of ball, dispersible tablet or gel health food：
Test result indicate that：Cake or tablet that the plant cell dry powder produced using the method is made, its content of mineral substances and
The cake or tablet effect that the more common dry powder of mouthfeel makes are more preferable.
Embodiment
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention
It is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, it is not intended to limit the present invention
Scope, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this
The various changes carried out on the premise of invention spirit and scope to the material component in these embodiments and consumption or change
Belong to protection scope of the present invention.Raw materials used and reagent of the invention is commercially available.
Embodiment 1
The solid culture of Vitis rich in mineral matter element
Choose Grape Skin in good condition and carry out plant cell callus induction, callus in good condition is gone to B5
Based on culture medium（Wherein sucrose containing 30g/L, 1mg/L 6-BA and 2mg/L NAA）Solid medium in continue cultivate.
During Vitis the 5th squamous subculture, three groups are classified as：
First group：Squamous subculture is into B5 basic solid medium（Wherein sucrose containing 30g/L, 1mg/L 6-BA and 2mg/L
NAA）；
Second group：Squamous subculture is into the basic solid mediums of B5 of high calcium ions and magnesium ions concentration（Wherein sucrose containing 30g/L, 1mg/
L 6-BA and 2mg/L NAA）, wherein CaCl2·2H2O concentration is 7.5 g/L, MgSO4·7H2O concentration is 2.5 g/L；
3rd group：Squamous subculture is into the basic solid mediums of B5 of high contents of calcium and magnesium zinc, iron, manganese ion concentration（Wherein sugarcane containing 30g/L
Sugar, 1mg/L 6-BA and 2mg/L NAA）, wherein CaCl2·2H2O concentration is 7.5 g/L, MgSO4·7H2O concentration is
2.5 g/L, ZnSO4·7H2O concentration is 20 mg/L, FeSO4·7H2O concentration is 278 mg/L.
Cultivate after 2 weeks, carry out the collection of Vitis in solid medium, be dried afterwards, dried powder is using former
Sub- absorption process carries out the analysis of Ca, Mg and Fe zinc ore material element, as a result as shown in table 2：
The B5 medium component list of table 1
Ca, Mg and Fe zinc ore material element content detection result in the solid culture Vitis of table 2
Embodiment 2
The suspension culture of Vitis rich in mineral matter element
Choose Grape Skin in good condition and carry out plant cell callus induction, callus in good condition is gone to B5
Based on culture medium（Wherein sucrose containing 30g/L, 1mg/L 6-BA and 2mg/L NAA）Fluid nutrient medium in continue cultivate.
During Vitis the 5th squamous subculture, three groups are classified as：
First group：Squamous subculture is into B5 basal liquid medium（Wherein sucrose containing 30g/L, 1mg/L 6-BA and 2mg/L
NAA）；
Second group：Squamous subculture is into the B5 basal liquid mediums of high calcium ions and magnesium ions concentration（Wherein sucrose containing 30g/L, 1mg/
L 6-BA and 2mg/L NAA）, wherein CaCl2·2H2O concentration is 7.5 g/L, MgSO4·7H2O concentration is 2.5 g/L；
3rd group：Squamous subculture is into the B5 basal liquid mediums of high contents of calcium and magnesium zinc, iron, manganese ion concentration（Wherein sugarcane containing 30g/L
Sugar, 1mg/L 6-BA and 2mg/L NAA）, wherein CaCl2·2H2O concentration is 7.5 g/L, MgSO4·7H2O concentration is
2.5 g/L, ZnSO4·7H2O concentration is 20 mg/L, FeSO4·7H2O concentration is 278 mg/L.
Cultivate after 2 weeks, carry out the collection of Vitis in fluid nutrient medium, be dried afterwards, dried powder is using former
Sub- absorption process carries out the analysis of Ca, Mg and Fe zinc-manganese mineral matter element, as a result as shown in table 3：
Ca, Mg and Fe zinc ore material element content detection result in the suspension for culturing grape cell of table 3
Embodiment 3
Suspend influence of the calcium-magnesium content to Vitis anthocyanidin content in cultivating
The culture medium based on B5（Wherein sucrose containing 30g/L, 1mg/L 6-BA and 2mg/L NAA）, in Grape Skin squamous subculture
During, respectively by the culture medium of its subculture to calcium and magnesium different ions concentration, CaCl in first group of culture medium2·2H2O's is dense
Spend for 0.15 g/L, MgSO4·7H2O concentration is 0.25 g/L；CaCl in second group of culture medium2·2H2O concentration is 1.5
G/L, MgSO4·7H2O concentration is that 0.25 g/L, i.e. calcium ion concentration improve 10 times；CaCl in 3rd group of culture medium2·2H2O
Concentration be 0.15 g/L, MgSO4·7H2O concentration is that 2.5 g/L, i.e. magnesium ion concentration improve 10 times；4th group of culture medium
Middle CaCl2·2H2O concentration is 1.5 g/L, MgSO4·7H2O concentration is 2.5 g/L, i.e. calcium ions and magnesium ions concentration is respectively increased
10 times；Respectively the 7th day of culture and the 10th day, Vitis collection is carried out, the relative OD of anthocyanidin in Vitis is determined
Value, as a result such as table 4：
Influence of the calcium magnesium element of table 4 to anthocyanidin in Vitis
Embodiment 4
The solid culture of Herba Saussureae Involueratae cell rich in mineral matter element
Choose Herba Saussureae Involueratae in good condition and carry out plant cell callus induction, by callus in good condition go to
Culture medium based on MS（Wherein sucrose containing 20g/L, 2mg/L 6-BA and 1mg/L NAA）Solid medium in continue cultivate.
In Herba Saussureae Involueratae cell the 5th squamous subculture, three groups are classified as：
First group：Squamous subculture is into MS basic solid medium（Wherein sucrose containing 20g/L, 2mg/L 6-BA and 1mg/L
NAA）；
Second group：Squamous subculture is into the basic solid mediums of MS of high calcium ions and magnesium ions concentration（Wherein sucrose containing 20g/L, 2mg/
L 6-BA and 1mg/L NAA）, wherein CaCl2·2H2O concentration is 22.0 g/L, MgSO4·7H2O concentration is 3.7 g/L；
3rd group：Squamous subculture is into the basic solid mediums of MS of high contents of calcium and magnesium zinc, iron, manganese ion concentration（Wherein sugarcane containing 20g/L
Sugar, 2mg/L 6-BA and 1mg/L NAA）, wherein CaCl2·2H2O concentration is 22.0 g/L, MgSO4·7H2O concentration is
3.7 g/L, ZnSO4·7H2O concentration is 86 mg/L, FeSO4·7H2O concentration is 278 mg/L.
Cultivate after 2 weeks, carry out the collection of solid medium central Tianshan saussurea involucrata cell, be dried afterwards, dried powder is adopted
The analysis of Ca, Mg and Fe zinc ore material element is carried out with atomic absorption method, as a result as shown in table 6：
The MS medium component tables of table 5
Ca, Mg and Fe zinc ore material element content detection result in the solid culture Herba Saussureae Involueratae cell of table 6
Embodiment 5
The suspension culture of Herba Saussureae Involueratae cell rich in mineral matter element
Choose Herba Saussureae Involueratae in good condition and carry out plant cell callus induction, by callus in good condition go to
Culture medium based on MS（Wherein sucrose containing 20g/L, 2mg/L 6-BA and 1mg/L NAA）Fluid nutrient medium in continue cultivate.
In Herba Saussureae Involueratae cell the 5th squamous subculture, three groups are classified as：
First group：Squamous subculture is into MS basal liquid medium（Wherein sucrose containing 20g/L, 2mg/L 6-BA and 1mg/L
NAA）；
Second group：Squamous subculture is into the MS basal liquid mediums of high calcium ions and magnesium ions concentration（Wherein sucrose containing 20g/L, 2mg/
L 6-BA and 1mg/L NAA）, wherein CaCl2·2H2O concentration is 22.0 g/L, MgSO4·7H2O concentration is 3.7 g/L；
3rd group：Squamous subculture is into the MS basal liquid mediums of high contents of calcium and magnesium zinc, iron, manganese ion concentration（Wherein sugarcane containing 20g/L
Sugar, 2mg/L 6-BA and 1mg/L NAA）, wherein CaCl2·2H2O concentration is 22.0 g/L, MgSO4·7H2O concentration is
3.7 g/L, ZnSO4·7H2O concentration is 86 mg/L, FeSO4·7H2O concentration is 278 mg/L.
Cultivate after 2 weeks, carry out the collection of fluid nutrient medium central Tianshan saussurea involucrata cell, be dried afterwards, dried powder is adopted
The analysis of Ca, Mg and Fe zinc ore material element is carried out with atomic absorption method, as a result as shown in table 7：
Table 7, which suspends, cultivates Ca, Mg and Fe zinc ore material element content detection result in Herba Saussureae Involueratae cell
Embodiment 6
Suspend influence of the calcium-magnesium content to Herba Saussureae Involueratae cell Syringin content in cultivating
The culture medium based on MS（Wherein sucrose containing 20g/L, 2mg/L 6-BA and 1mg/L NAA）, in the training of Herba Saussureae Involueratae subculture
During supporting, respectively by the culture medium of its subculture to calcium and magnesium different ions concentration, CaCl in first group of culture medium2·2H2O's
Concentration is 440 mg/L, MgSO4·7H2O concentration is 370 mg/L；CaCl in second group of culture medium2·2H2O concentration is
4.4 g/L, MgSO4·7H2O concentration is that 370 mg/L, i.e. calcium ion concentration improve 10 times；CaCl in 3rd group of culture medium2·
2H2O concentration is 440 mg/L, MgSO4·7H2O concentration is that 3.7 g/L, i.e. magnesium ion concentration improve 10 times；4th tissue culture
Support CaCl in base2·2H2O concentration is 4.4 g/L, MgSO4·7H2O concentration is that 3.7 g/L, i.e. calcium ions and magnesium ions concentration are distinguished
Improve 10 times；Respectively the 7th day of culture and the 10th day, Herba Saussureae Involueratae cell collection is carried out, determines purple in Herba Saussureae Involueratae cell
The content of syringin, as a result such as table 8：
Influence of the calcium magnesium element of table 8 to Syringin content in Herba Saussureae Involueratae cell
Embodiment 7
Preparation rich in mineral matter element Vitis pressed candy
Weigh the g of Vitis dry powder 100, it is the g of glucose 40, the g of D-sorbite 150, the g of xylitol 10, the g of acesulfame potassium 0.1, hard
The g of fatty acid magnesium 5, the g of microcrystalline cellulose 15, after being well mixed, using dry granulation direct tablet compressing, the every g of weight 0.3.
Embodiment 8
Preparation rich in mineral matter element Vitis capsule
Weigh the g of Vitis dry powder 300, the g of calcium lactate 5, the g of zinc sulfate 3, vitamin D 0.5g, the g of vitamin E 1, tristearin
The sour g of magnesium 15, it is well mixed after, filling using capsule machine, each capsule weighs 0.44 g.
Embodiment 9
Making rich in mineral matter Herba Saussureae Involueratae cell cake
Get out the g of flour 300, the g of Herba Saussureae Involueratae cell dry powder 50, the g of cream 30, the g of milk 200, the g of white granulated sugar 200, chicken
15, egg, butter are some, standby.The yolk and egg white of egg are separated, egg white is stirred to foaming, 140 g white sands are put into afterwards
Sugar, wherein remaining white granulated sugar, butter, milk are put into yolk, mixing and stirring, forms egg milk paste；By flour and day
Mountain saussurea involucrata cell dry powder blend is uniform, crosses 200 mesh sieves, and the flour of sieving and Herba Saussureae Involueratae cell powder are put into egg milk paste, filled
Divide stirring, then egg white is put into, stir, baking box is put into afterwards and carries out cake baking.
Embodiment 10
The making of Vitis Yoghourt
Prepare plain chocolate 200 mL, 1 g of g, VC of Vitis powder 20, the g of white granulated sugar 10, lactobacillus bulgaricus and thermophilic chain
The g of coccus active microbe powder 5 is standby.By Vitis powder boiling waterbath, filtration sterilization, adds milk and white granulated sugar, stirring afterwards
Uniformly, lactobacillus bulgaricus and streptococcus thermophilus are added afterwards, ferment 6-10 h at normal temperatures, you can obtain plant cell acid
Milk.
Embodiment 11
The making of Vitis beverage
Prepare 20 g Vitis powder, the g of white granulated sugar 20, the g of citric acid 1 standby, by Vitis powder by Vitis powder boiling water
Bath is extracted, and supernatant is filtered to take afterwards, is added white granulated sugar and citric acid, is added water and be settled to 100 mL, you can obtain Vitis drink
Material.
Claims (6)
1. a kind of culture plant cell method for producing high content mineral matter element and its application, it is characterised in that by following step
It is rapid to carry out：
（1）Plant cell callus is induced：The explants such as aseptic seedling, young leaves, stem, root that skin or seed are sprouted are planted from plant
Body is set out, and the ratio evoked callus of hormone needed for by adjusting plant cell growth is produced；
（2）Plant cell squamous subculture：
Plant cell solid medium squamous subculture：Plant cell chooses some solids after solid medium culture 10-40 days
The preferable plant cell of character is inoculated into new preparation solid medium in culture medium, that is, completes solid medium inoculation；
Plant cell liquid culture medium squamous subculture：The preferable plant cell of character in solid medium is transferred to Liquid Culture
Base culture, in liquid fresh plant cell culture medium culture 7-30 days, the seed cell after culture is passed through in Biohazard Safety Equipment
80 mesh sieve net filtrations, the 5.0-10.0 g wet cells after filtering are inoculated with 100ml/500ml shaking flasks, that is, complete plant cell
The squamous subculture of fluid nutrient medium；
（3）The addition of culture medium Mineral Elements in Jadeite Shellfish:
The addition of solid plant cell culture mineral matter element：In the preparation of solid plant Subculture culture medium, in original
Have on the basis of culture medium, characteristic improves the ion concentration of certain in solid medium or multi mineral prime element according to demand
5-100 times, carry out solid plant Subculture；
The addition of liquid plant cell culture mineral matter element：, can be in original training in liquid plant Cell Subculture
Support on the basis of base, characteristic improves the ion concentration 5- of certain in solid medium or multi mineral prime element according to demand
100 times, carry out liquid plant Subculture；Certain can also be added disposably or in multiple times during culture plant cell
The solion of kind or multi mineral prime element, it is 5-100 times to add multiple, continues culture plant cell；
（4）Culture plant cell：Solid plant cell is placed in culture plant cell case and cultivated, and collects cell after 10-40 days, enters
Row drying process, obtains dried plant cell powder, afterwards using the content of mineral substances in aas determination plant cell；
Liquid Culture plant cell is placed in shaken cultivation on shaking table or in suitable bioreactor culture, 10-30 DEG C or so training
Harvested after supporting 7-30 days, processing is dried, dried plant cell powder is obtained, afterwards using aas determination plant cell
In content of mineral substances；
（5）Using plant cell dried powder, add related auxiliary material and prepare relevant food or health food.
2. the plant cell described in claim 1 includes：Herba Saussureae Involueratae cell, Vitis, but it is not limited to both cells,
Also include being also not necessarily limited to ginseng-cell, apple cells, Saussurea laniceps cell, American Ginseng cell etc..
3. the mineral matter element described in claim 1 includes：Calcium, magnesium, iron, zinc, but these four mineral matter elements are not limited to, including
Copper, manganese, selenium, cobalt or other metal-chelator forms.
4. the incubation of the liquid plant cell described in claim 1 refers to the whole plant cell growth cycle.
5. the plant cell dry powder of the high content mineral matter element produced using method described in claim 1 is for making cake
Select, the application in terms of candy, dairy products, beverage or fermented wine.
6. the plant cell dry powder of the high content mineral matter element produced using method described in claim 1 for tablet,
Application in terms of capsule, granule, dripping pill, dispersible tablet or gel health food.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710290484.1A CN106947730B (en) | 2017-04-28 | 2017-04-28 | Plant cell culture method for producing high-content mineral elements and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710290484.1A CN106947730B (en) | 2017-04-28 | 2017-04-28 | Plant cell culture method for producing high-content mineral elements and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106947730A true CN106947730A (en) | 2017-07-14 |
| CN106947730B CN106947730B (en) | 2020-03-27 |
Family
ID=59477734
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710290484.1A Active CN106947730B (en) | 2017-04-28 | 2017-04-28 | Plant cell culture method for producing high-content mineral elements and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106947730B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107646685A (en) * | 2017-10-30 | 2018-02-02 | 江苏凤谷生物有限公司 | A kind of method for industrializing Herba Saussureae Involueratae culture of the large-scale production rich in flavones |
| CN113265368A (en) * | 2021-04-27 | 2021-08-17 | 陶氏益农生物工程（青岛）有限公司 | Plant cell culture method for producing high-content mineral elements |
| EP4067479A4 (en) * | 2019-11-28 | 2023-01-11 | Dalian Practical Biotechnology Co., Ltd. | Method for increasing the content of flavonoid phenylpropanoid compounds in saussurea involucrata cell culture |
| CN115786232A (en) * | 2022-12-19 | 2023-03-14 | 中国科学院青岛生物能源与过程研究所 | Rapid suspension culture and genetic transformation method of saussurea involucrate cells |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI130781B1 (en) * | 2022-08-26 | 2024-03-14 | Teknologian Tutkimuskeskus Vtt Oy | Method for production of cell-cultured ingestion products |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1112604A (en) * | 1994-05-26 | 1995-11-29 | 中国林业科学研究院亚热带林业研究所 | Ginseng tissue and cell germanium-enriching culture |
| CN101904303A (en) * | 2010-07-13 | 2010-12-08 | 大连普瑞康生物技术有限公司 | Chinese yew cell culture and method for large-scale subculture of same |
-
2017
- 2017-04-28 CN CN201710290484.1A patent/CN106947730B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1112604A (en) * | 1994-05-26 | 1995-11-29 | 中国林业科学研究院亚热带林业研究所 | Ginseng tissue and cell germanium-enriching culture |
| CN101904303A (en) * | 2010-07-13 | 2010-12-08 | 大连普瑞康生物技术有限公司 | Chinese yew cell culture and method for large-scale subculture of same |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107646685A (en) * | 2017-10-30 | 2018-02-02 | 江苏凤谷生物有限公司 | A kind of method for industrializing Herba Saussureae Involueratae culture of the large-scale production rich in flavones |
| EP4067479A4 (en) * | 2019-11-28 | 2023-01-11 | Dalian Practical Biotechnology Co., Ltd. | Method for increasing the content of flavonoid phenylpropanoid compounds in saussurea involucrata cell culture |
| CN113265368A (en) * | 2021-04-27 | 2021-08-17 | 陶氏益农生物工程（青岛）有限公司 | Plant cell culture method for producing high-content mineral elements |
| CN115786232A (en) * | 2022-12-19 | 2023-03-14 | 中国科学院青岛生物能源与过程研究所 | Rapid suspension culture and genetic transformation method of saussurea involucrate cells |
| CN115786232B (en) * | 2022-12-19 | 2023-10-24 | 中国科学院青岛生物能源与过程研究所 | Quick suspension culture and genetic transformation method for saussurea involucrata cells |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106947730B (en) | 2020-03-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106947730A (en) | A kind of culture plant cell method for producing high content mineral matter element and its application | |
| CN101756216B (en) | Yeast nutritious food and preparation method thereof | |
| CN102511720B (en) | Stachyose infant nourishing cereal and its preparation method | |
| CN107125736A (en) | A kind of preparation method of raspberry ferment | |
| CN106617051A (en) | Fruit and vegetable ferment freeze-dried powder and preparation method thereof | |
| CN102040409B (en) | Processing method of organic liquid fertilizer | |
| CN102885163B (en) | Tuckahoe-flavored functional cheese cake and preparation method thereof | |
| CN103908475A (en) | Traditional Chinese medicine preparation method | |
| CN106819109A (en) | The preparation method that a kind of sour milk powder freezes | |
| CN106465750A (en) | A kind of Rhizoma Solani tuber osi Yoghourt and preparation method thereof | |
| CN106085799A (en) | A kind of multi-cultur es co-immobilization fermentation Fructus Mori fruit vinegar and preparation method thereof | |
| CN106010979A (en) | Novel strain of flammulina fennae and cultivation method of novel strain | |
| CN102166005A (en) | Kefir yogurt of selenium-rich sprouted beans and production method thereof | |
| CN103598331B (en) | Rehmannia root functional yoghourt, rehmannia root blueberry functional yoghourt and preparation method thereof | |
| CN107466860A (en) | A kind of Chinese yew FOS lactic bacteria activity beverage and preparation method thereof | |
| CN106689391A (en) | Sour soybean milk with high viable count of lactic acid bacteria and high acidity, and preparation method of sour soybean milk | |
| CN109329922A (en) | The preparation method of golden Rosa roxburghii Tratt ferment | |
| CN108703287A (en) | A kind of whitening enzyme beverage and preparation method thereof containing small-molecular peptides Yu a variety of enzymes | |
| CN109700007A (en) | A kind of Organic Lemon ferment and its preparation process | |
| CN108850170A (en) | A kind of red bean Semen Coicis Yoghourt and preparation method thereof | |
| CN104430867A (en) | Preparation method of living bacterium type red jujube acid milk beverage | |
| CN110679660A (en) | Liver-protecting and hangover-alleviating wolfberry polysaccharide yoghourt and preparation method thereof | |
| CN102894081A (en) | Sweet potato dietary fiber yoghourt | |
| CN102047960A (en) | Shrubalthea flower yoghurt and preparation method thereof | |
| CN105028661A (en) | Method for preparing soybean milk fermented milk by lactobacillus strains capable of producing high-yield folic acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200608 Address after: 310000 No.71 Wulin Road, Xiacheng District, Hangzhou City, Zhejiang Province Patentee after: Lu Qikang Address before: 300457 Tianjin international biological medicine Joint Research Institute, No. 220 Dongting Road, thirteen street, Tanggu District, Tianjin, N602 Patentee before: TIANJIN ACELBIO BIOTECHNOLOGY Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201113 Address after: No.19, 7th floor, building 1, No.18, Jinli East Road, Qingyang District, Chengdu City, Sichuan Province 610015 Patentee after: Wu Jianbo Patentee after: Lu Qikang Address before: 310000 No.71 Wulin Road, Xiacheng District, Hangzhou City, Zhejiang Province Patentee before: Lu Qikang |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210224 Address after: 400000 No.8 Fengqi Road, caijiagang Town, Beibei District, Chongqing Patentee after: Ansapo (Chongqing) Biotechnology Co.,Ltd. Address before: No.19, 7th floor, building 1, No.18 Jinli East Road, Qingyang District, Chengdu, Sichuan 610015 Patentee before: Wu Jianbo Patentee before: Lu Qikang |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240806 Address after: Floors 1-3, Building 5, No. 10 Xingde Road, Zengjia Town, Shapingba District, Chongqing, China 401331 Patentee after: Chongqing Ansaibo New Material Technology Co.,Ltd. Country or region after: China Address before: 400000 No.8 Fengqi Road, caijiagang Town, Beibei District, Chongqing Patentee before: Ansapo (Chongqing) Biotechnology Co.,Ltd. Country or region before: China |