CN106942351A - A kind of method for disinfection of fresh white fungus - Google Patents
A kind of method for disinfection of fresh white fungus Download PDFInfo
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- CN106942351A CN106942351A CN201710083825.8A CN201710083825A CN106942351A CN 106942351 A CN106942351 A CN 106942351A CN 201710083825 A CN201710083825 A CN 201710083825A CN 106942351 A CN106942351 A CN 106942351A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/015—Preserving by irradiation or electric treatment without heating effect
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
Abstract
The invention provides a kind of method for disinfection of fresh white fungus, comprise the following steps：Step 1：By curcumin spray solution on fresh white fungus；Step 2：The fresh white fungus LED visible light that step 1 has sprayed curcumin solution is irradiated, sterilization process is completed.The present invention uses cooperatively food additives curcumin with nontoxic LED visible light, can at utmost kill the microorganism on fresh white fungus, by verification experimental verification, sterilization rate may be up to 99%, and bactericidal effect is good；Relative to expensive hypocrelline B or inedible porphyrin, sterilizing cost will can be greatly reduced, simultaneously as food additives, curcumin that is cheap and being excited using common LED visible ray as sensitising agent, curcumin belongs to natural plant extracts, safe source；The method of the present invention belongs to nom-thermal sterilization, while to the sterilization of fresh white fungus, can at utmost keep nutritional ingredient, excellent flavor and the mouthfeel of fresh white fungus, effective Shelf-life.
Description
Technical field
The present invention relates to food processing technology field, more particularly to a kind of method for disinfection of fresh white fungus.
Background technology
White fungus, also known as tremella, tremella, white fungus etc., belong to Mycophyta Tremellaceae Tremella, the U.S. for having " hat in bacterium "
Claim, be one of " ginseng, young pilose antler, swallow, ear ", with extremely high nutritive value.There is plantation white fungus in most of China area, main production
Ground is that Fujian, Sichuan, Hubei, Shaanxi, Zhejiang, Guizhou etc. are saved, and with Fujian at most, especially there is Gutian of " township of white fungus " good reputation
County.Fresh white fungus moisture content is high, it is tender and crisp to organize, easily occur corrupt smelly phenomenon, should not store up long.Cause fresh white fungus it is putrid and deteriorated,
The principal element of reduced shelf-life is the growth and breeding of microorganism.
At present, the common sterilization technology of fresh white fungus is mainly traditional heat sterilization, although can effectively kill micro- life
Thing, but the taste and flavor of fresh white fungus is destroyed to a certain extent, cause the loss of nutrient, it is impossible to meet vast consumption
The demand of person.As improvement, the China patent of Application No. 201310565509.6 discloses a kind of storage of fresh white fungus
Preservation method, including selection, the preparation of prevent-browning Nanutal food fresh-keeping agent and the processing of fresh white fungus, controlled atmospheric packing (MAP), ray
Or electron beam irradiation sterilization, cryopreservation to outbound, listing, wherein it is sterilized using ray or electron beam irradiation, belong to non-heating power
It is sterilized, although preferably to save the nutritional ingredient of fresh white fungus to a certain extent, but radiation sterilizing needs Special Equipment to produce
Radiation simultaneously ensures that safety measure ensures that radiation is not revealed, and cost is higher, while, it is necessary to strict control irradiation metering, can lead
The distinctive fragrance loss of white fungus is caused, and usually needs to be packaged into pouch-packaged and can just irradiate, workload is considerably increased.
Therefore, it is necessary to which it is good and can preferably keep the fresh silver of fresh white fungus original local flavor and mouthfeel to invent a kind of bactericidal effect
The method for disinfection of ear.
The content of the invention
It is an object of the invention to overcome drawbacks described above, there is provided a kind of bactericidal effect is good and can preferably keep fresh white fungus
The method for disinfection of the fresh white fungus of original local flavor and mouthfeel.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention is：
A kind of method for disinfection of fresh white fungus, comprises the following steps：
Step 1：By curcumin spray solution on fresh white fungus；
Step 2：The fresh white fungus LED visible light that step 1 has sprayed curcumin solution is irradiated, sterilization process is completed.
A kind of method for disinfection of fresh white fungus, comprises the following steps：
Step 1：At room temperature, by 10~50umol/L curcumins spray solution on fresh white fungus；
Step 2：At room temperature, by step 1 sprayed curcumin solution fresh white fungus LED visible light irradiation 5~
120min, completes sterilization process.
The beneficial effects of the present invention are：Food additives curcumin is used cooperatively with nontoxic LED visible light, can be most
Big degree kills the microorganism on fresh white fungus, and by verification experimental verification, sterilization rate may be up to 99%, and bactericidal effect is good；Relative to valency
Lattice expensive hypocrelline B or inedible porphyrin, can be as food additives, cheap and use commonly
The curcumin that LED visible light can be excited greatly reduces sterilizing cost as sensitising agent, meanwhile, curcumin belongs to natural plant
Thing extract, safe source；Curcumin have can effectively be inactivated after preferable photosensitive activity, photosensitizer it is micro- in fresh white fungus
Biology, the non-heating power bacteria reducing technology of photosensitizer that it is mediated is safer relative to conventional sterilization methods；The method of the present invention has
Spectrum sterilization idiocratic, and it belongs to nom-thermal sterilization, is confirmed through overtesting, the inventive method sterilized to fresh white fungus while,
Nutritional ingredient, excellent flavor and the mouthfeel of fresh white fungus, effective Shelf-life can at utmost be kept.
Brief description of the drawings
Fig. 1 be the fresh white fungus of the embodiment of the present invention 1 method for disinfection in various concentrations curcumin solution to fresh white fungus clump count
Influence.
Fig. 2 be the fresh white fungus of the embodiment of the present invention 2 method for disinfection in the different irradiation time of LED visible light to fresh white fungus bacterium colony
Several influences.
Fig. 3 be the fresh white fungus of the embodiment of the present invention 3 method for disinfection in the fresh white fungus of the cold sterilization processing of light power total bacterium kill
Go out effect.
Embodiment
To describe the technology contents of the present invention in detail, feature, the objects and the effects being constructed, below in conjunction with embodiment
And coordinate accompanying drawing to be explained in detail.
At present, the disease (mainly for cancer) that light power technology is mainly used in medically cures field；It is rarely used for food
Product manufacture field, only Tang Qing it is beautiful wait report by light source of LED, curcumin for sensitising agent light power cold sterilization technology can be used for it is male
Oyster is fresh-keeping, but so far, relevant report of the light dynamic pasteurization technology in edible fungus fresh-keeping technical field is not yet found, because oyster is
A kind of biology being stored in liquid, need to can only soak sensitising agent is soluble in water oyster and fresh-keeping use.And fresh white fungus
Due to its inherent characteristic, not to soak form presentation in daily life, therefore, light power nonthermal technology is killed to edible mushroom
Bacterium effect, the correlative factor for influenceing the bactericidal effect and its unclear, the institute to edible mushroom local flavor itself, the influence of mouthfeel etc.
So that the present invention is by improving spray modes action pathway, further sterilization of the research light power nonthermal technology to edible mushroom
Effect, the correlative factor for influenceing the bactericidal effect and its to edible mushroom local flavor itself, influence of mouthfeel etc..
The design of most critical of the present invention is：Fresh white fungus is irradiated using curcumin as sensitising agent and by light source of LED visible light.
Fig. 1 to Fig. 3 is refer to, a kind of method for disinfection of fresh white fungus comprises the following steps：
Step 1：By curcumin spray solution on fresh white fungus；
Step 2：The fresh white fungus LED visible light that step 1 has sprayed curcumin solution is irradiated, sterilization process is completed.
The present invention principle be：Light irradiation is excited the sensitising agent of tissue resorption, and the sensitising agent handle of excitation state
Oxidation reaction occurs for oxygen of the energy transmission to surrounding, generation activity very strong singlet oxygen, singlet oxygen and adjacent large biological molecule,
Cytotoxic effect is produced, and then causes cell damage or even death, so as to reach the purpose for killing invasive organism；By ginger
Flavine is to be extracted from turmeric in the stem tuber of turmeric, curcuma zedoary, root tuber of aromatic turmeric etc. as sensitising agent, curcumin (Curcumin)
A kind of natural yellow aldehydes matter, is the main active component that turmeric plays pharmacological action.Japan, print are originated in curcumin more
The torrid zones such as degree, China, subtropical countries, are one of current seven big natural food colours the most salable in the world, belong to natural
Plant extracts, raw material sources are wide, low cost, and safety non-toxic is pollution-free.Research confirms that curcumin has anti-inflammatory, anti-freezing, resisted
Oxidation, reducing blood lipid, cholagogic, anticancer etc. are acted on, and with strong broad-spectrum antibacterial action, to staphylococcus aureus, large intestine
Bacillus etc. has strong inhibitory action.
Knowable to foregoing description, beneficial effects of the present invention are：By food additives curcumin and nontoxic LED visible light
Use cooperatively, can at utmost kill the microorganism on fresh white fungus, by verification experimental verification, sterilization rate may be up to 99%, sterilization effect
It is really good；, can be low as food additives, price relative to expensive hypocrelline B or inedible porphyrin
The honest and clean and curcumin that can be excited using common LED visible ray greatly reduces sterilizing cost as sensitising agent, meanwhile, turmeric
Element belongs to natural plant extracts, safe source；Curcumin had and can effectively be inactivated after preferable photosensitive activity, photosensitizer
Microorganism in fresh white fungus, the non-heating power bacteria reducing technology of photosensitizer that it is mediated is safer relative to conventional sterilization methods；This hair
Bright method has spectrum sterilization idiocratic, and it belongs to nom-thermal sterilization, is confirmed through overtesting, and the inventive method is to fresh white fungus
While sterilized, nutritional ingredient, excellent flavor and the mouthfeel of fresh white fungus, effective Shelf-life can be at utmost kept.
Further, the concentration of curcumin solution described in step 1 is LED visible light photograph in 10~50umol/L, step 2
The time penetrated is 5~120min.
Seen from the above description, the concentration and LED visible light irradiation time of sensitising agent curcumin solution are controlled simultaneously
Above range can obtain more preferable bactericidal effect.
Further, the concentration of the curcumin solution is 40umol/L, and the time of LED visible light irradiation is 60min.
Seen from the above description, the time that the concentration of curcumin solution and LED visible light are irradiated is controlled respectively
During 40umol/L and 60min, by verification experimental verification, while the fresh original local flavor of white fungus of holding is taken into account with mouthfeel, sterilization effect
Most preferably.
Further, the curcumin in the curcumin solution is food-grade curcumin, and the purity of the curcumin is
More than 93.5%.
Seen from the above description, fresh white fungus can be sprayed directly on as the raw material of curcumin solution from food-grade curcumin
On, using safer convenience, control the purity of curcumin to be prevented effectively from the case where ensureing photosensitive activity and spray excessively
The aqueous solution is on fresh white fungus, it is to avoid because excessive water content causes rotting for fresh white fungus.
Further, step 1 and step 2 are carried out at room temperature.
A kind of method for disinfection of fresh white fungus, comprises the following steps：
Step 1：In under room temperature environment, by 10~50umol/L curcumins spray solution on fresh white fungus；
Step 2：In under room temperature environment, by step 1 sprayed the fresh white fungus LED visible light irradiation 5 of curcumin solution~
120min, completes sterilization process.
The present invention principle be：Light irradiation is excited the sensitising agent of tissue resorption, and the sensitising agent handle of excitation state
Oxidation reaction occurs for oxygen of the energy transmission to surrounding, generation activity very strong singlet oxygen, singlet oxygen and adjacent large biological molecule,
Cytotoxic effect is produced, and then causes cell damage or even death, so as to reach the purpose for killing invasive organism；By ginger
Flavine is to be extracted from turmeric in the stem tuber of turmeric, curcuma zedoary, root tuber of aromatic turmeric etc. as sensitising agent, curcumin (Curcumin)
A kind of natural yellow aldehydes matter, is the main active component that turmeric plays pharmacological action.Japan, print are originated in curcumin more
The torrid zones such as degree, China, subtropical countries, are one of current seven big natural food colours the most salable in the world, belong to natural
Plant extracts, raw material sources are wide, low cost, and safety non-toxic is pollution-free.Research confirms that curcumin has anti-inflammatory, anti-freezing, resisted
Oxidation, reducing blood lipid, cholagogic, anticancer etc. are acted on, and with strong broad-spectrum antibacterial action, to staphylococcus aureus, large intestine
Bacillus etc. has strong inhibitory action.
Knowable to foregoing description, beneficial effects of the present invention are：By food additives curcumin and nontoxic LED visible light
Use cooperatively, can at utmost kill the microorganism on fresh white fungus, by verification experimental verification, sterilization rate may be up to 99%, sterilization effect
It is really good；, can be low as food additives, price relative to expensive hypocrelline B or inedible porphyrin
The honest and clean and curcumin that can be excited using common LED visible ray greatly reduces sterilizing cost as sensitising agent, meanwhile, turmeric
Element belongs to natural plant extracts, safe source；Curcumin had and can effectively be inactivated after preferable photosensitive activity, photosensitizer
Microorganism in fresh white fungus, the non-heating power bacteria reducing technology of photosensitizer that it is mediated is safer relative to conventional sterilization methods；This hair
Bright method has spectrum sterilization idiocratic, and it belongs to nom-thermal sterilization, is confirmed through overtesting, and the inventive method is to fresh white fungus
While sterilized, nutritional ingredient, excellent flavor and the mouthfeel of fresh white fungus, effective Shelf-life can be at utmost kept.
Further, the concentration of the curcumin solution is 40umol/L, and the time of LED visible light irradiation is 60min.
Further, the curcumin in the curcumin solution is food-grade curcumin, and the purity of the curcumin is
More than 93.5%.
Embodiment 1
Various concentrations curcumin solution influences on fresh white fungus sterilization effect
Step 1：Under 28 DEG C of environmental conditions, quantitative fresh white fungus is weighed as experimental group and blank control group；With nothing
The water-reducible curcumin of bacterium, prepares the curcumin solution that final concentration is respectively 10,20,40 and 50umol/L, then with 10,20,
40 and 50umol/L curcumin solution sprays the fresh white fungus of each experimental group respectively, and blank control group is not processed；
Step 2：Under 28 DEG C of environmental conditions, experimental group fresh white fungus LED light source is irradiated into 60min, blank control group is not
Process.Every group of setting two is parallel.
Compliance test result：Bacterium is carried out to the fresh white fungus of above-mentioned experimental group and blank control group using bacterial plate counts method respectively
Fall sum and calculate (sterilization effect evaluation), as a result see Fig. 1.As shown in Figure 1, when LED visible light irradiation time is identical, curcumin
The concentration of solution is 10umol/L and 20umol/L, and the non-heat sterilization of curcumin-photosensitizer has to the microorganism in fresh white fungus
Certain deactivation, effect and non-optimal；When the concentration of curcumin solution is 40umol/L and 50umol/L, in fresh white fungus
Total plate count can reduce by 2 orders of magnitude, and inactivation ratio is up to 99%.
Embodiment 2
Influence of the LED visible light difference irradiation time to fresh white fungus sterilization effect
Step 1：Under 28 DEG C of environmental conditions, quantitative fresh white fungus is weighed as experimental group and blank control group；With nothing
The water-reducible curcumin of bacterium, configures 40umol/L curcumin solution, is then tested with 40umol/L curcumin spray solution
The fresh white fungus of group, blank control group is not processed；
Step 2：Under 28 DEG C of environmental conditions, by the fresh white fungus LED light source of experimental group irradiate respectively 5,30,60,90 and
120min, blank control group is not processed.Every group of setting two is parallel.
Compliance test result：Bacterium is carried out to the fresh white fungus of above-mentioned experimental group and blank control group using bacterial plate counts method respectively
Fall sum and calculate (sterilization effect evaluation), as a result see Fig. 2.As can be seen from Figure 2, when curcumin concentration is 40umol/L, as LED can
See that the total plate count in the extension of the irradiation time of light, fresh white fungus shows the trend maintained an equal level after elder generation significantly successively decreases.Work as sensitising agent
Concentration is 40umol/L, when light application time is 60min, and the inactivation ratio of total bacterium is up to 99% in fresh white fungus, and sterilization effect is notable.
Embodiment 3
To the killing test of the total bacterium of fresh white fungus
Under 28 DEG C of environmental conditions, weigh quantitative fresh white fungus and be used as blank control group (being left intact), simple
Light group (do not spray curcumin and only carry out illumination), simple sensitising agent group (sprinkling 40umol/L curcumins but without illumination) and
Light dynamic experiment group (sprinkling 40umol/L curcumins simultaneously carry out 60min illumination).
Compliance test result：Bacterium is carried out to the fresh white fungus of above-mentioned experimental group and blank control group using bacterial plate counts method respectively
Fall sum and calculate (sterilization effect evaluation), as a result see Fig. 3.As can be seen from Figure 3, the total plate count of blank control group and simple light group
Total plate count it is substantially unchanged, the total plate count of simple sensitising agent group has been reduced, but DeGrain, shows sensitising agent sheet
The inactivation ratio that body has the fresh total bacterium of white fungus in certain bactericidal effect, light dynamic experiment group may be up to 99%, show that power is handled
There is significant bactericidal effect afterwards.
Embodiment 4
The sense organ change experiment of fresh white fungus after light dynamic pasteurization before processing
Sensitising agent pre-processes fresh white fungus：Fresh white fungus is handled with 40umol/L curcumin.
LED visible light is irradiated：The fresh white fungus handled with curcumin is placed under LED light source and irradiates 1h, sterilization process is completed.
The sense organ change of fresh white fungus：Fresh white fungus after the processing of contrast photosensitizer, observes outward appearance, color and luster, the change of smell respectively.
As a result show, fresh white fungus still overall full, N/D in terms of outward appearance after photosensitizer processing；It is in terms of color and luster
Existing fresh white fungus is distinctive slightly yellow, and uniform color；In terms of smell, the distinctive fragrance of fresh white fungus, fragrance are still kept.
Embodiment 5
The physical and chemical mutagenesis experiment of fresh white fungus after light dynamic pasteurization before processing
Sensitising agent pre-processes fresh white fungus：Fresh white fungus is divided into two groups, one group is control group, one group of turmeric with 40umol/L
It is plain to be handled.
LED visible light is irradiated：The fresh white fungus handled with curcumin is placed under LED light source and irradiates 60min, is completed sterilized
Journey.Control group is without illumination.
(1) fresh white fungus moisture change：A certain amount of fresh white fungus is taken to carry out determination of moisture in control group and experimental group respectively.
(2) fresh white fungus chromatic aberration：A certain amount of fresh white fungus is taken to be carried out using color difference meter in control group and experimental group respectively
Color difference measurement.
(3) fresh white fungus firmness change：Taking a certain amount of fresh white fungus in control group and experimental group respectively, (P2 is visited using Texture instrument
Head) carry out Determination of Hardness.
As a result show, the moisture of (1) control group and the fresh white fungus of experimental group is 90% or so, and without significant difference,
Illustrate photosensitizer processing on fresh white fungus moisture without influence；(2) the L* values of control group and the fresh white fungus of experimental group are 55.35 or so,
And without significant difference, illustrate the non-heating power bacteria reducing technical finesse of light power fresh white fungus and control group fresh white fungus in color indifference
It is different；(3) hardness number of control group and the fresh white fungus of experimental group is 105.208 or so, and indifference.Show to work as from physical and chemical experiment result
Curcumin concentration is 40umol/L, when light application time is 60min, the physical and chemical index of control group and the fresh white fungus of photosensitizer experimental group
Without significant change, the characteristics of fresh white fungus is original is maintained.
In summary, the method for disinfection for the fresh white fungus that the present invention is provided, can by food additives curcumin and nontoxic LED
See that light is used cooperatively, can at utmost kill the microorganism on fresh white fungus, by verification experimental verification, sterilization rate may be up to 99%, kill
Bacterium effect is good；Relative to expensive hypocrelline B or inedible porphyrin, food additives, valency can be used as
Lattice are cheap and the curcumin that can be excited using common LED visible ray is as sensitising agent, greatly reduce sterilizing cost, meanwhile,
Curcumin belongs to natural plant extracts, safe source；Curcumin has can be effectively after preferable photosensitive activity, photosensitizer
The microorganism in fresh white fungus is inactivated, the non-heating power bacteria reducing technology of photosensitizer that it is mediated is safer relative to conventional sterilization methods；
The method of the present invention has spectrum sterilization idiocratic, and it belongs to nom-thermal sterilization, is confirmed through overtesting, and the inventive method is to fresh
While white fungus is sterilized, nutritional ingredient, excellent flavor and the mouthfeel of fresh white fungus, effective Shelf-life can be at utmost kept.
Embodiments of the invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize this hair
Equivalent structure or equivalent flow conversion that bright specification and accompanying drawing content are made, or directly or indirectly it is used in other related skills
Art field, is included within the scope of the present invention.
Claims (8)
1. a kind of method for disinfection of fresh white fungus, it is characterised in that comprise the following steps：
Step 1：By curcumin spray solution on fresh white fungus；
Step 2：The fresh white fungus LED visible light that step 1 has sprayed curcumin solution is irradiated, sterilization process is completed.
2. the method for disinfection of fresh white fungus according to claim 1, it is characterised in that curcumin solution described in step 1
Concentration is 10~50umol/L, and the time that LED visible light is irradiated in step 2 is 5~120min.
3. the method for disinfection of fresh white fungus according to claim 2, it is characterised in that the concentration of the curcumin solution is
40umol/L, the time of LED visible light irradiation is 60min.
4. the method for disinfection of fresh white fungus according to claim 1, it is characterised in that the curcumin in the curcumin solution
For food-grade curcumin, the purity of the curcumin is more than 93.5%.
5. the method for disinfection of fresh white fungus according to claim 1, it is characterised in that step 1 and step 2 are entered at room temperature
OK.
6. a kind of method for disinfection of fresh white fungus, it is characterised in that comprise the following steps：
Step 1：At room temperature, by 10~50umol/L curcumins spray solution on fresh white fungus；
Step 2：At room temperature, the fresh white fungus LED visible light that step 1 has sprayed curcumin solution is irradiated into 5~120min, it is complete
Into sterilization process.
7. the method for disinfection of fresh white fungus according to claim 6, it is characterised in that the concentration of the curcumin solution is
40umol/L, the time of LED visible light irradiation is 60min.
8. the method for disinfection of fresh white fungus according to claim 6, it is characterised in that the curcumin in the curcumin solution
For food-grade curcumin, the purity of the curcumin is more than 93.5%.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107889884A (en) * | 2017-11-21 | 2018-04-10 | 福建农林大学 | A kind of fresh lotus seeds become sour the control method of swollen bag |
| CN109517226A (en) * | 2019-01-07 | 2019-03-26 | 福建农林大学 | A kind of light power bacteria reducing preservative film for packaged instant jellyfish |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103583670A (en) * | 2013-11-13 | 2014-02-19 | 中国农业科学院农产品加工研究所 | Method for storage and freshness preservation of fresh tremella |
| CN103991920A (en) * | 2014-05-20 | 2014-08-20 | 刘一鸣 | Photodynamic-based drinking water disinfection method |
| CN104304408A (en) * | 2014-11-17 | 2015-01-28 | 中国海洋大学 | Photodynamic cold sterilizing and fresh-keeping method |
| CN204670345U (en) * | 2015-06-04 | 2015-09-30 | 刘一鸣 | A kind of fruit vegetable cleaning and sterilization machine |
| CN204861064U (en) * | 2015-07-24 | 2015-12-16 | 刘一鸣 | Novel light power fruit cleaning and disinfecting machine |
| CN105230775A (en) * | 2015-10-30 | 2016-01-13 | 通江古林银耳有限公司 | High-efficiency preserving agent for fresh white fungus, preparation method thereof and preservation method thereof |
-
2017
- 2017-02-16 CN CN201710083825.8A patent/CN106942351A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103583670A (en) * | 2013-11-13 | 2014-02-19 | 中国农业科学院农产品加工研究所 | Method for storage and freshness preservation of fresh tremella |
| CN103991920A (en) * | 2014-05-20 | 2014-08-20 | 刘一鸣 | Photodynamic-based drinking water disinfection method |
| CN104304408A (en) * | 2014-11-17 | 2015-01-28 | 中国海洋大学 | Photodynamic cold sterilizing and fresh-keeping method |
| CN204670345U (en) * | 2015-06-04 | 2015-09-30 | 刘一鸣 | A kind of fruit vegetable cleaning and sterilization machine |
| CN204861064U (en) * | 2015-07-24 | 2015-12-16 | 刘一鸣 | Novel light power fruit cleaning and disinfecting machine |
| CN105230775A (en) * | 2015-10-30 | 2016-01-13 | 通江古林银耳有限公司 | High-efficiency preserving agent for fresh white fungus, preparation method thereof and preservation method thereof |
Cited By (18)
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| CN107889884A (en) * | 2017-11-21 | 2018-04-10 | 福建农林大学 | A kind of fresh lotus seeds become sour the control method of swollen bag |
| CN109517226A (en) * | 2019-01-07 | 2019-03-26 | 福建农林大学 | A kind of light power bacteria reducing preservative film for packaged instant jellyfish |
| CN109517226B (en) * | 2019-01-07 | 2021-03-30 | 福建农林大学 | Photodynamic bacterium-reducing preservative film for packaging instant jellyfishes |
| CN109717352A (en) * | 2019-01-30 | 2019-05-07 | 广东温氏佳味食品有限公司 | Bacteria reducing method, photosensitive water-cooling system and its application of Tang Pin |
| CN109717352B (en) * | 2019-01-30 | 2022-07-05 | 广东温氏佳味食品有限公司 | Soup sterilization method, photosensitive water cooling system and application thereof |
| CN110074170B (en) * | 2019-06-10 | 2023-01-06 | 吉林大学 | Fresh-keeping method of photodynamic therapy combined with N-succinyl chitosan composite coating liquid |
| CN110074170A (en) * | 2019-06-10 | 2019-08-02 | 吉林大学 | The preservation method of photodynamic therapy combination N- succinyl-chitosan compelx coating liquid |
| CN111328952A (en) * | 2020-03-03 | 2020-06-26 | 四川大学 | Photodynamic sterilization method for acidic food |
| CN111328952B (en) * | 2020-03-03 | 2023-04-25 | 四川大学 | Photodynamic sterilization method for acidic food |
| CN112120138A (en) * | 2020-09-18 | 2020-12-25 | 中国农业大学 | Photodynamic sterilization device and method for food powder |
| CN112602535A (en) * | 2020-12-25 | 2021-04-06 | 广东润源中天生物科技有限公司 | Ganoderma lucidum culture medium and culture method |
| CN114403209A (en) * | 2021-12-27 | 2022-04-29 | 中国农业大学 | Method for processing plant source food raw material |
| CN114287474A (en) * | 2022-01-13 | 2022-04-08 | 福建农林大学 | Fresh-keeping method for prolonging shelf life of hypsizygus marmoreus by high-voltage static activation photodynamic |
| CN114287474B (en) * | 2022-01-13 | 2023-09-29 | 福建农林大学 | Fresh-keeping method for prolonging shelf life of hypsizygus marmoreus by high-voltage electrostatic activation photodynamic force |
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| CN115191476A (en) * | 2022-06-28 | 2022-10-18 | 江苏大学 | Dry pepper pulse strong light combined curcumin photodynamic sterilization method |
| CN115191476B (en) * | 2022-06-28 | 2024-02-13 | 江苏大学 | Photodynamic sterilization method combining dry chilli pulse strong light with curcumin |
| CN115590062A (en) * | 2022-08-31 | 2023-01-13 | 广西中医药大学（Cn） | Application of emodin as photosensitizer in photodynamic sterilization |
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Application publication date: 20170714 |