CN106942048A - A kind of hybridizing method for improving distant hybridization peanut pollination success rate - Google Patents
A kind of hybridizing method for improving distant hybridization peanut pollination success rate Download PDFInfo
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- CN106942048A CN106942048A CN201710242886.4A CN201710242886A CN106942048A CN 106942048 A CN106942048 A CN 106942048A CN 201710242886 A CN201710242886 A CN 201710242886A CN 106942048 A CN106942048 A CN 106942048A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/14—Boron; Compounds thereof
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/27—Dispersions, e.g. suspensions or emulsions
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Inorganic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of hybridizing method for improving distant hybridization peanut pollination success rate, comprise the following steps：(1) the male flower pollen of male parent is gathered in peanut full-bloom stage；(2) maternal pollen is collected in peanut florescence, high-temperature inactivation is carried out with thermostat water bath；(3) the male flower pollen for the male parent for gathering step (1) mid-term is mixed with the maternal inactivation pollen prepared in step (2), and pollen suspension is configured to sucrose+boric acid mixed solution；(4) pollen suspension is uniformly applied on the maternal pistil stigma of peanut；(5) routinely operate, carry out the preventing and controlling of pest and disease damage, until fruit maturation；(6) progeny selection, strain are carried out to compare, select elite plant strain to enter identification garden, the yield and quality qualification test of 2 years multiple spots is then carried out, obtains new peanut variety.The maternal pollen of the high-temperature inactivation of the present invention has stronger fusion-activity, uses it for the derivant of pollen fusion, the compatibility of pollen suspension is enhanced, so as to improve peanut hybridization success rate.
Description
Technical field
The invention belongs to peanut breeding technical field, and in particular to a kind of raising distant hybridization peanut pollination success rate it is miscellaneous
Friendship method.
Background technology
In distant hybridization peanut artificial hybridization breeding, every paternal pollen is attached to pistil stigma, due to male parent flower
The compatibility of powder and pistil stigma does not cause by force that xenogenesis pollen can not normally germinate on column cap, pollen tube can not after pollen germination
It is slow or too short into column cap, pollen tube growth, cause distant hybridization peanut hybridization success rate relatively low.
The content of the invention
The purpose of the present invention is to improve distant hybridization peanut there is provided one kind for above-mentioned the deficiencies in the prior art to pollinate successfully
The hybridizing method of rate.
The technical scheme is that realize in the following manner：
A kind of hybridizing method for improving distant hybridization peanut pollination success rate, comprises the following steps：
(1) the male flower pollen of male parent is gathered in peanut full-bloom stage；
(2) maternal pollen is collected in peanut florescence, with thermostat water bath in 42 DEG C~45 DEG C of temperature, kilogram/li of pressure 1
Rice2Under the conditions of carry out high-temperature inactivation 5~10 minutes；
(3) the male flower pollen for the male parent for gathering step (1) mid-term is mixed with the maternal inactivation pollen prepared in step (2)
Close, pollen suspension is configured to sucrose+boric acid mixed solution；
(4) pollen suspension is uniformly applied on the maternal pistil stigma of peanut；
(5) routinely operate, carry out the preventing and controlling of pest and disease damage, until fruit maturation；
(6) progeny selection, strain are carried out to compare, select elite plant strain to enter identification garden, the yield of 2 years multiple spots is then carried out
Quality Identification is tested, and obtains new peanut variety.
Mixed solution in the step (3) includes the boric acid of 0.5~1.5% sucrose+0.05~0.15%.
The present invention has advantages below：
(1) the maternal pollen of high-temperature inactivation of the invention has stronger fusion-activity, uses it for luring for pollen fusion
Agent is led, the compatibility of pollen suspension is enhanced, xenogenesis pollen normally germination, pollen tube after pollen germination on column cap can be enable
Enough enter column cap, pollen tube growth very fast or longer, so as to improve distant hybridization peanut hybridization success rate.
(2) sucrose of sucrose+boric acid mixed solution of the invention during pollen germination except energy can be provided for pollen
Outside source, moreover it is possible to play a part of adjusting osmotic pressure, maintain normal life power, be conducive to sprouting；Because boron ion can be with sucrose shape
Into complex compound, sugar is set to be easy to transport in the tissue by plasma membrane, so as to promote the absorption and metabolism of sugar, boron can participate in pectin in addition
The synthesis of material, beneficial to the construction of pollen tube wall, boron can form sugared boric acid complex, increase the absorption of oxygen, boron and sugared combination,
The germination rate of peanut pollen can be greatly improved.
Embodiment
Technical scheme is further illustrated below by specific embodiment.
A kind of hybridizing method for improving distant hybridization peanut pollination success rate, comprises the following steps：
(1) the male flower pollen of male parent is gathered in peanut full-bloom stage；
(2) maternal pollen is collected in peanut florescence, with thermostat water bath in 42 DEG C~45 DEG C of temperature, kilogram/li of pressure 1
Rice2Under the conditions of carry out high-temperature inactivation 5~10 minutes；The maternal pollen of high-temperature inactivation has stronger fusion-activity, uses it for
The derivant of pollen fusion, enhances the compatibility of pollen suspension, and can making xenogenesis pollen, normally germination, pollen are sprouted on column cap
Pollen tube can be very fast or longer into column cap, pollen tube growth after hair, so as to improve distant hybridization peanut hybridization success rate；
(3) the male flower pollen for the male parent for gathering step (1) mid-term is mixed with the maternal inactivation pollen prepared in step (2)
Close, pollen suspension is configured to sucrose+boric acid mixed solution；The sucrose of sucrose+boric acid mixed solution is during pollen germination
In addition to it can provide the energy for pollen, moreover it is possible to play a part of adjusting osmotic pressure, maintain normal life power, be conducive to sprouting；By
Sugar can be made to be easy to transport in the tissue by plasma membrane, so as to promote absorption and the generation of sugar with sucrose formation complex compound in boron ion
Thank, boron can participate in the synthesis of pectin substance in addition, beneficial to the construction of pollen tube wall, boron can form sugared boric acid complex, increase oxygen
Absorption, boron and sugar combination, the germination rate of peanut pollen can be greatly improved；
(4) pollen suspension is uniformly applied on the maternal pistil stigma of peanut；
(5) routinely operate, carry out the preventing and controlling of pest and disease damage, until fruit maturation；
(6) progeny selection, strain are carried out to compare, select elite plant strain to enter identification garden, the yield of 2 years multiple spots is then carried out
Quality Identification is tested, and obtains new peanut variety.
Mixed solution in the step (3) includes the boric acid of 0.5~1.5% sucrose+0.05~0.15%.
Above by the embodiment of embodiment form, the present invention is described in detail, but this should not be managed
Solve the embodiment that the above is only limitted to for above-mentioned subject area of the invention, it is all based on the above of the present invention it is achieved that
Technology belongs to the scope of the present invention.
Claims (2)
1. a kind of hybridizing method for improving distant hybridization peanut pollination success rate, it is characterised in that comprise the following steps：
(1) the male flower pollen of male parent is gathered in peanut full-bloom stage；
(2) maternal pollen is collected in peanut florescence, with thermostat water bath in 42 DEG C~45 DEG C of temperature, 1 kg/cm of pressure2Bar
High-temperature inactivation is carried out under part 5~10 minutes；
(3) the male flower pollen for the male parent for gathering step (1) mid-term is mixed with the maternal inactivation pollen prepared in step (2), is used
Sucrose+boric acid mixed solution is configured to pollen suspension；
(4) pollen suspension is uniformly applied on the maternal pistil stigma of peanut；
(5) routinely operate, carry out the preventing and controlling of pest and disease damage, until fruit maturation；
(6) progeny selection, strain are carried out to compare, select elite plant strain to enter identification garden, the yield and quality of 2 years multiple spots is then carried out
Qualification test, obtains new peanut variety.
2. according to the method described in claim 1, it is characterised in that：Mixed solution in the step (3) includes 0.5~
The boric acid of 1.5% sucrose+0.05~0.15%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710242886.4A CN106942048A (en) | 2017-04-08 | 2017-04-08 | A kind of hybridizing method for improving distant hybridization peanut pollination success rate |
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| CN201710242886.4A CN106942048A (en) | 2017-04-08 | 2017-04-08 | A kind of hybridizing method for improving distant hybridization peanut pollination success rate |
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| CN106942048A true CN106942048A (en) | 2017-07-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710242886.4A Pending CN106942048A (en) | 2017-04-08 | 2017-04-08 | A kind of hybridizing method for improving distant hybridization peanut pollination success rate |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107643365A (en) * | 2017-08-09 | 2018-01-30 | 中国农业科学院蜜蜂研究所 | A kind of construction method of tomato pollen vigor gradient |
| CN110100720A (en) * | 2019-05-21 | 2019-08-09 | 漯河市农业科学院 | A kind of selection of high yield colour peanut |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1394470A (en) * | 2002-06-07 | 2003-02-05 | 中国科学院昆明植物研究所 | Method for breeding michellia hybrid species |
| CN1813521A (en) * | 2005-09-28 | 2006-08-09 | 河南科技学院 | Pollen liquid utilizing method for cotton hybrid seeds production |
| CN104813920A (en) * | 2015-04-28 | 2015-08-05 | 吉林省农业科学院 | Method for improving peanut hybridization efficiency |
-
2017
- 2017-04-08 CN CN201710242886.4A patent/CN106942048A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1394470A (en) * | 2002-06-07 | 2003-02-05 | 中国科学院昆明植物研究所 | Method for breeding michellia hybrid species |
| CN1813521A (en) * | 2005-09-28 | 2006-08-09 | 河南科技学院 | Pollen liquid utilizing method for cotton hybrid seeds production |
| CN104813920A (en) * | 2015-04-28 | 2015-08-05 | 吉林省农业科学院 | Method for improving peanut hybridization efficiency |
Non-Patent Citations (7)
| Title |
|---|
| 农业部市场与经济信息司等: "《无公害皮苹果标准化生产手册》", 31 January 2008, 中国农业出版社 * |
| 冯社章: "《园艺植物花果管理技术》", 31 December 2012, 中国农业出版社 * |
| 周汉群等: "花生亲和种远缘杂交育种研究", 《花生学报》 * |
| 孙大容: "《花生育种学》", 31 July 1998, 中国农业出版社 * |
| 庄文庆: "《药用植物育种学》", 31 October 1993, 农业出版社 * |
| 张治安等: "《植物生理学》", 31 August 2011, 中国林业出版社 * |
| 禹山林: "《中国花生品种及其系谱》", 31 December 2008, 上海科学技术出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107643365A (en) * | 2017-08-09 | 2018-01-30 | 中国农业科学院蜜蜂研究所 | A kind of construction method of tomato pollen vigor gradient |
| CN107643365B (en) * | 2017-08-09 | 2020-09-22 | 中国农业科学院蜜蜂研究所 | Construction method of tomato pollen activity gradient |
| CN110100720A (en) * | 2019-05-21 | 2019-08-09 | 漯河市农业科学院 | A kind of selection of high yield colour peanut |
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Application publication date: 20170714 |