CN106939039A - The albumen related to paddy rice grain length and seed holding and its encoding gene and application - Google Patents

Abstract

The invention discloses a kind of albumen related to paddy rice grain length and seed holding and its encoding gene and application.Protein provided by the present invention, is named as GL4, is specially following any：A) protein shown in sequence 1；B) protein shown in sequence 2；C) amino acid sequence a) or b) limited is passed through to the substitution and/or missing and/or addition of one or several amino acid residues, and the protein related to the grain length and/or seed holding of vegetable seeds；D) and a) c) in any limited amino acid sequence there is more than 99%, more than 95%, more than 90%, more than 85% or more than 80% homology, and the protein related to the grain length and/or seed holding of vegetable seeds.GL4 albumen and its encoding gene provided by the present invention are significant in terms of the seed length and seed holding that improve vegetable seeds, will be played a significant role in high yield new variety of plant is cultivated.

Description

The albumen related to paddy rice grain length and seed holding and its encoding gene and application

Technical field

The invention belongs to plant genetic engineering field, it is related to a kind of albumen related to paddy rice grain length and seed holding and its volume Code gene and application.

Background technology

Oryza (Oryza L.) plant includes two particularly important raise crops, i.e. Asian Cultivated Rice (O.sativa L.) and Oryza glaberrima Steud (Oryza glaberrima Steud.), independently originating from Asia and Africa.

Oryza glaberrima Steud is the cultivated rice independently originating from Africa before about 3000, and Asian Cultivated Rice is by different Progenitor species are tamed.Asian Cultivated Rice is just introduced West Africa by 16 middle of century, Portuguese, causes the thing of Oryza glaberrima Steud Rapid reduction is planted, so present planting area and area are much smaller than Asian Cultivated Rice.At present, Asian Cultivated Rice is in global model Interior extensive plantation is enclosed, food source is provided for nearly half population in the world.Oryza glaberrima Steud limitation is distributed in the western part in Africa, is One of local main cereal crops, civilization and local economy to Africa serve positive effect.However, wide with having carried out The Asian Cultivated Rice of general research is compared, and the correlative study of Oryza glaberrima Steud is very deficient, and the colony of Oryza glaberrima Steud is lost so far Pass structure and evolutionary history is known little about it.

But, Oryza glaberrima Steud has the characteristic such as precocious, disease-resistant, drought-resistant, there is certain life in special ecological environment Production advantage.Therefore, the crop for improving Oryza glaberrima Steud has certain application value.

The content of the invention

It is an object of the invention to provide a kind of albumen related to paddy rice grain length and seed holding and its encoding gene and application.

Protein provided by the present invention, is named as GL4, is specially following any：

A) protein being made up of the amino acid sequence shown in sequence in sequence table 1；

B) protein being made up of the amino acid sequence shown in sequence in sequence table 2；

C) substitution and/or missing by amino acid sequence a) or b) limited by one or several amino acid residues And/or addition, and the protein related to the grain length and/or seed holding of vegetable seeds；

D) and a)-c) in any limited amino acid sequence have more than 99%, more than 95%, more than 90%, 85% Above or more than 80% homology, and the protein related to the grain length and/or seed holding of vegetable seeds.

Wherein, the protein shown in (c) and (d) can be the protein from paddy rice.

, can be as follows in amino terminal or the carboxyl terminal connection of the protein for the ease of the protein purification Label shown in table.

Table：The sequence of label

The nucleic acid molecules of code for said proteins fall within protection scope of the present invention.

The nucleic acid molecules can be DNA, such as cDNA, genomic DNA or recombinant DNA；The nucleic acid molecules can also be RNA, such as mRNA, hnRNA or tRNA.

In one embodiment of the invention, the nucleic acid molecules are specially the gene of code for said proteins, the base Because concretely it is following it is any shown in DNA molecular：

1) DNA molecular in sequence table shown in 2341-4346 of sequence 3；

2) DNA molecular in sequence table shown in 2322-4333 of sequence 4；

3) DNA molecular in sequence table shown in sequence 5；

4) DNA molecular in sequence table shown in sequence 6；

5) under strict conditions with 1) -4) in any restriction DNA molecular hybridization and DNA points of code for said proteins Son；

6) with 1) -5) in any restriction DNA sequence dna have more than 99%, more than 95%, more than 90%, more than 85% or The homology of person more than 80%, and the DNA molecular of code for said proteins.

Above-mentioned stringent condition can be that with 6 × SSC, 0.5%SDS solution hybridizes at 65 DEG C, then with 2 × SSC, 0.1%SDS and 1 × SSC, 0.1%SDS respectively wash film once.

Recombinant vector, expression cassette, transgenic cell line or recombinant bacterium containing above-mentioned nucleic acid molecules fall within the guarantor of the present invention Protect scope.

The recombinant vector can be recombinant expression carrier, or recombinant cloning vector.

The recombinant expression carrier can use existing plant expression vector construction.The plant expression vector includes double base agriculture Bacillus carrier and carrier available for plant micropellet bombardment etc., such as pCAMBIA1300, pCUbi1390, pCHF3, PGreen0029, pCAMBIA3301, pBI121, pBin19, pCAMBIA2301, pCAMBIA1301-UbiN or other derivative are planted Thing expression vector.The plant expression vector can also include 3 ' end untranslated regions of foreign gene, i.e., believe comprising polyadenylic acid Number and any other participations mRNA processing or gene expression DNA fragmentation.The bootable polyadenylic acid of polyadenylation signals adds Enter the 3 ' ends to mRNA precursor.During using the gene constructed recombinant expression carrier, it can be added before its transcription initiation nucleotides Any enhanced, composing type, organizing specific type or inducible promoter, such as cauliflower mosaic virus (CAMV) 35S are opened Mover, ubiquitin gene Ubiquitin promoters (pUbi), stress induced promoter rd29A etc., they can be used alone or with Other plant promoters are used in combination；In addition, when using the gene constructed recombinant expression carrier of the present invention, it is also possible to use enhancing Son, including translational enhancer or transcriptional enhancer, these enhancer regions can be ATG initiation codon or neighboring region starting Codon etc., but must be identical with the reading frame of coded sequence, to ensure the correct translation of whole sequence.The translation control letter Number and the source of initiation codon be extensive, can be natural or synthesis.Translation initiation region can come from Transcription initiation region or structural gene., can be to used for the ease of transgenic plant cells or plant are identified and screened Recombinant expression carrier is processed, and the enzyme or luminophor of color change can be produced as added the coding that can be expressed in plant Gene, resistant antibiotic marker or anti-chemical reagent marker gene etc..Also any selected marker can be not added with Gene, directly screens transformed plant with adverse circumstance.

In the present invention, start the promoter of the genetic transcription in the recombinant expression carrier for paddy rice is endogenous to start Son, specifically as shown in 1-2321 of sequence 4 in sequence table or 19-2340 of sequence 3.

More specifically, in one embodiment of the invention, the recombinant expression carrier is in pCAMBIA1300 carriers Multiple cloning sites at the restructuring matter that is obtained after DNA fragmentation shown in sequence 4 in (be specially XbaI and HindIII) insetion sequence table Grain.In another embodiment of the present invention, the recombinant expression carrier be by DNA fragmentation shown in sequence in sequence table 3 with The recombinant plasmid of gained after pCAMBIA1300 carrier homologous recombinations.

The expression cassette is by that can start the promoter of the gene expression, the gene, and transcription terminator group Into.

The protein or the nucleic acid molecules or the recombinant vector, expression cassette, transgenic cell line or recombinant bacterium exist It is following it is any in application fall within protection scope of the present invention：

(a) the seed length of vegetable seeds is increased；

(b) the increased plant variety of seed selection seed length；

(c) seed holding of vegetable seeds is improved；

(d) plant variety that seed selection seed holding is improved.

Wherein, the plant that the method for the increased plant variety of seed selection seed length and the seed selection seed holding are improved The method of kind, specifically may include using the higher plant of the gene expression amount as parent hybridized the step of.

The present invention also protects a kind of method for cultivating the plant that the increase of seed length and/or seed holding are improved.

The method provided by the present invention for cultivating the plant that the increase of seed length and/or seed holding are improved, it may include as follows Step：Protein expression amount and/or activity described in claim 1 in recipient plant are improved, so as to obtain the increase of seed length And/or the plant that seed holding is improved.

The present invention also protects a kind of method for cultivating genetically modified plants.

The method provided by the present invention for cultivating genetically modified plants, the step of specifically may include following (a1) and (a2)：

(a1) encoding gene of the protein is imported into recipient plant, obtains expressing the transgenosis of the encoding gene Plant；

(a2) obtained in genetically modified plants compared with the recipient plant obtained by the step (a1), with following (b1) and/ Or the genetically modified plants of character shown in (b2)：

(b1) seed length increase；

(b2) seed holding is improved.

Expression quantity of the protein in the genetically modified plants is higher than the recipient plant；The gene is concretely Following any shown DNA molecular：

1) DNA molecular in sequence table shown in 2341-4346 of sequence 3；

2) DNA molecular in sequence table shown in 2322-4333 of sequence 4；

3) DNA molecular in sequence table shown in sequence 5；

4) DNA molecular in sequence table shown in sequence 6；

5) under strict conditions with 1) -4) in any restriction DNA molecular hybridization and DNA points of code for said proteins Son；

6) with 1) -5) in any restriction DNA sequence dna have more than 99%, more than 95%, more than 90%, more than 85% or The homology of person more than 80%, and the DNA molecular of code for said proteins.

Above-mentioned stringent condition can be that with 6 × SSC, 0.5%SDS solution hybridizes at 65 DEG C, then with 2 × SSC, 0.1%SDS and 1 × SSC, 0.1%SDS respectively wash film once.

The gene can be specifically imported in the recipient plant by any of the above-described recombinant expression carrier, obtain described Genetically modified plants.Specifically can be by using Ti-plasmids, Ri plasmids, plant viral vector, directly delivered DNA, microinjection, electricity Lead, the recombinant expression carrier is converted plant cell or tissue by the conventional biology methods such as agriculture bacillus mediated, particle gun, and will The plant tissue of conversion cultivates into plant.

In the present invention, the plant can be monocotyledon, such as grass, specific such as paddy rice, it is more specific such as African paddy rice.

In one embodiment of the invention, the recipient plant is specially Oryza glaberrima Steud kind IRGC102305.

It is demonstrated experimentally that recombinant expression of proteins carrier shown in sequence 1 in expressible nucleotide sequence table or sequence 2 is converted into Africa Rice varieties IRGC102305 is cultivated, with the Oryza glaberrima Steud kind IRGC102305 plant phases without transgenosis under the same terms Than the seed length of transgenic paddy rice is longer, and seed holding is stronger.GL4 albumen and its encoding gene provided by the present invention are being carried It is significant in terms of the seed length and seed holding of high vegetable seeds, it will play important in high yield new variety of plant is cultivated Effect.

Brief description of the drawings

Fig. 1 is that strain GIL25 is compared with African rice varieties IRGC102305 seed length and seed holding.

Fig. 2 is GL4 assignment of genes gene mapping schematic diagrames.

Fig. 3 be embodiment 2 in be transferred to pGL4-1 carriers transgenic line PCR qualification results.

Fig. 4 is the seed that the transgenic lines of pGL4-1 carriers and African rice varieties IRGC102305 are transferred in embodiment 2 Length and seed holding compare.In figure, pGL4-1 represents to be transferred to the transgenic line of pGL4-1 carriers.

Fig. 5 is the seed length statistics of different genetic stocks in embodiment 2.

Fig. 6 is the seed that the transgenic lines of pGL4-2 carriers and African rice varieties IRGC102305 are transferred in embodiment 3 Length and seed holding compare.In figure, pGL4-2 represents to be transferred to the transgenic line of pGL4-2 carriers.

Fig. 7 is the seed length statistics of different genetic stocks in embodiment 3.

Embodiment

Experimental method used in following embodiments is conventional method unless otherwise specified.

Material, reagent used etc., unless otherwise specified, are commercially obtained in following embodiments.

African rice varieties IRGC102305 and African wild rice W1411：It can be obtained from International Rice, network address is：

http://www.irgcis.irri.org:81/grc/irgcishome.html；Or

https://shigen.nig.ac.jp/rice/oryzabase/strain/wildCore/list。

PCAMBIA1300 carriers：Be recorded in " Zhu Zuofeng, Tan Lubin, Fu Yongcai, Liu Fengxia, Cai Hongwei,Xie Daoxin,Wu Feng,Wu Jianzhong,Matsumoto Takashi,Sun Chuanqing.Genetic control of inflorescence architecture during rice domestication.Nature Commun,2013,4:2200doi:The text of 10.1038/ncomms3200. " one, the public can be from Obtained at applicant, can only be used to repetition present invention experiment and use.

The positioning of embodiment 1, the African rice seed size of regulation and control and seed holding gene

African rice varieties IRGC102305 hybridizes with African wild rice W1411, then using IRGC102305 as recurrent parent, Repeatedly backcrossing obtain one on seed size with the obvious strain GIL25 of IRGC102305 difference, and its seed easily fall (figure 1), it is hybridized with IRGC102305 and constructs a F2 segregating population for including 186 individual plants.In this F2 colony, seed Grain length and seed holding are all with marking RM3335 and RM5608 close linkages, and by the GL4 assignments of genes gene mapping between the two genes. Then segregating population is expanded to 6500 individual plants, it is found that seed holding is isolated with seed size.And according to mark RM3335 with Primers between RM5608, most at last the GL4 assignments of genes gene mapping to mark M3 and M4 between 5.9kb on (Fig. 2).And institute The strain for having seed longer shows seed holding, and the shorter strain of seed shows not seed holding.In website http:// Ensembl.gramene.org/Oryza_glaberrima is upper according to Oryza glaberrima Steud IRGC96717 (CG14) sequence prediction, The interval comprises only a candidate gene ORGLA04G0254300.Therefore the gene is exactly GL4 genes, controls Oryza glaberrima Steud Seed length.Strain GIL25 is identical with African wild rice W1411 GL4 gene orders.

The acquisition and its functional verification of the African rice of embodiment 2, GL4 transgenosis

First, the structure of expression vector

According to African wild rice W1411 gene ORGLA04G0254300 and promoter sequence design primer, and drawing Thing two ends introduce restriction enzyme XbaI and HindIII recognition site and protection base respectively, and primer sequence is as follows：

pGL4-1F：5’-TGCTCTAGA(band underscore base is restriction enzyme to GCTGGCCGTAGAAGTCAAG-3 ' XbaI recognition sites and protection base, sequence thereafter are 1-19 of sequence 4)；

pGL4-1R：5’-ACAAAGCTT(band underscore base is restriction enzyme to GTATAGCTCAGCACGAGC-3 ' HindIII recognition sites and protection base, sequence thereafter are the reverse complementary sequence of 4876-4893 of sequence 4).

African wild rice W1411 complete genome DNA is extracted using CTAB methods.Using this DNA as template, amplification length is about 4.8kb DNA fragmentation, its sequence is " TGCTCTAGA+ sequences 4+AAGCTTTGT ".Reclaimed with kit, be cloned into plant Between thing expression vector pCAMBIA1300 restriction enzyme site XbaI and HindIII, the plant expression for obtaining Oryza glaberrima Steud is carried Body, pGL4-1 is named as after sequencing is correct.

PGL4-1 structure is described as follows：DNA fragmentation shown in sequence in sequence table 4 is replaced to pCAMBIA1300 digestion The recombinant plasmid obtained after small fragment between site XbaI and HindIII.

1-2321 of sequence 4 are the endogenous GL4 gene promoter sequences of African wild rice W1411,2322-4333 Position is the GL4 gene orders containing introne (3156-3994 are intron sequences).The corresponding GL4 without introne Gene C DS sequences are as shown in sequence 6 in sequence table.Sequence in 2322-4333 of sequence 4 and the equal polynucleotide of sequence 6 GL4 albumen shown in row 2.

2nd, conversion Oryza glaberrima Steud and PCR identifications

The carrier pGL4-1 that step one is built by Bombardment-Mediated Transformation enter seed it is shorter and not shattering Africa cultivate Rice varieties IRGC102305,2 wheel screenings are carried out with the NB culture mediums containing hygromycin, then through pre- differentiation, differentiation obtains transgenosis plant Strain.Experiment sets the control that pCAMBIA1300 empty carriers are transferred into Oryza glaberrima Steud kind IRGC102305 simultaneously.

Transfer-gen plant is identified by PCR.Concrete operations are as follows：Take growth period transformed plant and empty vector control plant With Oryza glaberrima Steud kind IRGC102305 blade, STb gene is extracted using CTAB methods, is directed to using following primer The hygromycin gene carried on pCAMBIA1300 carriers enters performing PCR identification：

HptF：5’-gtctccgacctgatgcagctctcgg-3’；

HptR：5’-gtccgtcaggacattgttggag-3’.

Qualification result as shown in figure 3, swimming lane 1 be DNA Marker III (Beijing Tuo Yingfang Science and Technology Ltd.s product), Purpose band size is 520bp.Swimming lane 2-15 is transgenic positive plant, and swimming lane 16 is distilled water, and swimming lane 17 is non-transgenosis Oryza glaberrima Steud kind IRGC102305, swimming lane 18 be positive control pCAMBIA1300 carriers.

3rd, the Function Identification of transgenosis rice

12 plants of transfer-gen plants are obtained altogether through the screening of step 2 hygromycin resistance and PCR identifications.Planted with this 12 plants of transgenosis Strain, and it is transferred to the control of pCAMBIA1300 empty carriers and the Oryza glaberrima Steud kind IRGC102305 without transgenosis processing For experiment material.The seed phenotype and seed holding of each experiment material are observed, and counts 3 transgenic lines, 3 unloaded controls The seed length of strain and Oryza glaberrima Steud kind IRGC102305, is each 10 plants.

As a result show：

It is mould through step 2 tide compared with the Oryza glaberrima Steud kind IRGC102305 handled with unloaded control and without transgenosis The all longer seeds of its seed of transfer-gen plant of plain resistance screening and PCR identification, and it is more easy to shattering (Fig. 4).

The seed of 3 transgenic lines of statistics, 3 unloaded control strains and Oryza glaberrima Steud kind IRGC102305 is long Degree, as a result as shown in Figure 5.Wherein, pGL4-1-1, pGL4-1-2 and pGL4-1-3 represent 3 transgenic lines randomly selected, 3 unloaded control strains that vector-1, vector-2 and vector-3 represent to randomly select, IRGC102305-1 represent without The Oryza glaberrima Steud kind IRGC102305 of transgenosis processing.As seen from the figure, Oryza glaberrima Steud IRGC102305 seed length For 8.25 centimetres, the positive individual plant seed length for being transferred to empty carrier is 8.21-8.27 centimetres, and the positive for being transferred to pGL4-2 carriers is single Strain seed length is 9.36-9.42 centimetres.The positive individual plant seed length for being transferred to pGL4-2 is single relative to the positive for being transferred to zero load Strain and acceptor material IRGC102305 are significantly improved, and (t is test, P<0.05).Therefore, the GL4 albumen (sequences shown in sequence 2 2322-4333 of 4 or the GL4 genes shown in sequence 6) it can be used for regulation and control seed length and seed holding.

Embodiment 3, the acquisition and checking of crucial variation

First, the acquisition of crucial variation

Expressed using qPCR method more African wild rice W1411 and Oryza glaberrima Steud kind IRGC102305 GL4 Level discovery, GL4 expression quantity no significant differences in both materials.To African wild rice W1411 and Oryza glaberrima Steud kind The coding region sequence of IRGC102305 GL4 genes, which is compared, to be found, one has variation at five：G294A, C766T, C1466T, (variation is Oryza glaberrima Steud kind IRGC102305 relative to African wild rice W1411 to A1515G and GCCGCC681-686 missings For).Compared and found by the GL4 gene orders of 16 parts of wild rices and 67 parts of Oryza glaberrima Steuds, C766T and seed length with And seed holding correlation degree highest.

2nd, the structure of expression vector

Genome using Oryza glaberrima Steud kind IRGC102305 designs following two primer pair pGL4-2Af/ as template PGL4-2aR and pGL4-2bF/pGL4-2bR, wherein with underscore and be runic base to be artificially introduced, in order that replying C766T be mutated, italic and with underscore part be pCAMBIA1300 carrier sequences.

pGL4-2aF：5’-TACCCGGGGATCCTCTAGGCTGGCCGTAGAAGTCAAG-3’；

pGL4-2aR：5’-CGACGACGGCGGCGGCTGAGGAGGAGGTGGGTGGTG-3’。

pGL4-2bF：5’-AGCCGCCGCCGTCGTCGCTGCA-3’；

pGL4-2bR：5’-TGTAAAACGACGGCCAGTGCCACTTGTATAGCTCAGCACGAGC-3’。

Genome using Oryza glaberrima Steud kind IRGC102305 is template, respectively with primer pair pGL4-2Af/pGL4- 2aR and pGL4-2bF/pGL4-2bR are expanded, and it is about 3.1kb and 1.8kb fragment that length is obtained respectively, after gel extraction, The two fragments are connected with pCAMBIA1300 carriers by homologous recombination enzyme, obtain one comprising GL4 upstreams 2kb and Downstream 560bp recombinant vector, pGL4-2 is named as after sequencing is correct.Fragment embedded by pGL4-2 carriers removes above-mentioned C766T Position is different from Oryza glaberrima Steud IRGC102305 outer, and other sequences are identical with IRGC102305.In C766T positions, carry Body pGL4-2 is base C, and Oryza glaberrima Steud kind IRGC102305 is base T.

The structure of pGL4-2 carriers is described as：DNA fragmentation shown in sequence in sequence table 3 and pCAMBIA1300 carriers is same Recombinant vector obtained by source restructuring (upstream and downstream homology arm is respectively 1-18 and 4906-4931 of sequence 3) afterwards.

Wherein, 1-18 of sequence 3 are the sequence on pCAMBIA1300 carriers, 19-2340 with 4906-4931 Position is the endogenous GL4 gene promoter sequences of Oryza glaberrima Steud kind IRGC102305, and 2341-4346 are GL4 genes (the 3169-4007 are intron sequences).The corresponding GL4 gene C DS sequences without introne are as shown in sequence 5 in sequence table. GL4 albumen in 2341-4346 of sequence 3 and the equal polynucleotide of sequence 5 shown in sequence 1.

3rd, conversion Oryza glaberrima Steud and PCR identifications

The carrier pGL4-2 that step one is built by Bombardment-Mediated Transformation enter seed it is shorter and not shattering Africa cultivate Rice varieties IRGC102305, carried out with the NB culture mediums containing hygromycin 2 wheel screening, then through with differentiation, differentiation obtain transgenosis plant Strain.Experiment sets the control that pCAMBIA1300 empty carriers are transferred into Oryza glaberrima Steud kind IRGC102305 simultaneously.Pass through PCR identifies transfer-gen plant.The specific such as step 2 of embodiment 2.

4th, the Function Identification of transgenosis rice

15 plants of transfer-gen plants are obtained altogether through the screening of step 2 hygromycin resistance and PCR identifications.Planted with this 15 plants of transgenosis Strain, and it is transferred to the control of pCAMBIA1300 empty carriers and the Oryza glaberrima Steud kind IRGC102305 without transgenosis processing For experiment material.The seed phenotype and seed holding of each experiment material are observed, and counts 3 transgenic lines, 3 unloaded controls The seed length of strain and Oryza glaberrima Steud kind IRGC102305, is each 10 plants.

As a result show：

It is mould through step 2 tide compared with the Oryza glaberrima Steud kind IRGC102305 handled with unloaded control and without transgenosis Plain resistance screening and PCR identification obtains 15 plants of all longer seeds (Fig. 6) of its seed of transfer-gen plant altogether.

The seed of 3 transgenic lines of statistics, 3 unloaded control strains and Oryza glaberrima Steud kind IRGC102305 is long Degree, as a result as shown in Figure 7.Wherein, pGL4-2-1, pGL4-2-2 and pGL4-2-3 represent 3 transgenic lines randomly selected, 3 unloaded control strains that vector-1, vector-2 and vector-3 represent to randomly select, IRGC102305-1 represent without The Oryza glaberrima Steud kind IRGC102305 of transgenosis processing.As seen from the figure, Oryza glaberrima Steud IRGC102305 seed length For 8.25 centimetres, the positive individual plant seed length for being transferred to empty carrier is 8.21-8.27 centimetres, and the positive for being transferred to pGL4-2 carriers is single Strain seed length is 9.24-9.28 centimetres.The positive individual plant seed length for being transferred to pGL4-2 is single relative to the positive for being transferred to zero load Strain and acceptor material IRGC102305 are significantly improved, and (t is test, P<0.05).Therefore, the GL4 albumen (sequences shown in sequence 1 2341-4346 of 3 or the GL4 genes shown in sequence 5) it can be used for regulation and control seed length and seed holding.

<110>China Agricultural University

<120>The albumen related to paddy rice grain length and seed holding and its encoding gene and application

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<170> PatentIn version 3.5

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caaaaaacgc cggaaaacgc attgccgagc gagccgtttc agcaaataag ggatcgttag 240

actttatcag tgactgtatg aaacttttat atatgtgtgt gttcttagga cttaaaagat 300

aatgctgaaa aatttatttc aaaatttaaa ttttggtttt gacttattat aaatttttgg 360

ggcaaccgat ggggctatga ggctatgagc tatccatcca tccatagcgc aacgtgtgac 420

agcgtgaggt gacaagatgg aaaaccgtga gccgcgccac cggtgtaaac cccctccctt 480

cacgcgcgtt tactttgcaa gcaaaggaca aggaaaaaag ccgtaattgg cgaagaatgt 540

tcttggattt gattagcagc ggtacggtaa cacctctagc ttgcatttgc atggtagggt 600

gatttggcta ggagagcagg atcatccacg cattcaattt tcctcatttc tttttgcagg 660

ctcaatagag gagaggaggg gcctagctag ccccaagata atgcagcaaa aaggagagaa 720

gagaacatgt catccagtcc aaagtgcaaa cgtctctata caaatggaac atatggagtg 780

gacctaagcc tagttgcttc tgtgtcccaa gctcccaagt gtcaaagcct taatgtgcaa 840

aaccacatct gtggggactc ctgaaagcaa actaaatgat tgcaaaagag ttcgtttatt 900

cacctattta tttatttatt ttattttctt tccatgtgtc tgttgtttga tactttgatt 960

gatcatgtgc taaactgcag aaaaaaccaa caggcccaga gtaccggcga aaaggctcct 1020

cgatgaaaag tgcagggatt ctgggggagt tgccagtgcg ccaccgttgg atttttgcac 1080

acatataaac agggctctgc tgcacctctc acatagtaac atatataggg acggttccac 1140

caagcacgtt tgggaaatgg ttttttgagc aacatctcat gcgaacaatt taggcactat 1200

ttacttgtac cttcaccttg tacgtgtgct ccatcccata gctgcattat ttgcataaat 1260

ccttttaatt gcatatatac atactctctc catatttttt ttaataaatg gcgtatttca 1320

ctttttatca caccatgtct tttaataaga cgaaatatca aatgtgtgac aaaaaaatca 1380

ataatgtcat ctattaaaag acagattaag tatatgagag gcatttaatt atttgccacc 1440

tttgatgttt tacaatttat cactcgtgtg acatagacgc atagggtaac aaattgcgaa 1500

acatcaagta atctgtgagt agcaaatact taaatatctc ctaatatgtt agcattgcac 1560

ccaactttat atcaaaactc tataactata ttgagctttg tcctgcccat ggtaaagtgt 1620

ggggttaaag tgttaaaaag ctattggtag cagtatctag tatggcgtgg caggccggtg 1680

cagataaaag gggccccggg ttttgcccgg aaatgaggag aggacgcccc ggcagctagc 1740

ggacgctttg catttgcatg catggtgctc tctacttctc ccctccgttc ccacggcaaa 1800

tgcaaaacgc atgctagtat ttggcaccat tagcctaaga tagtagtatc agagtgttac 1860

agaaagaata taattatgta gtagtatagt aattaattaa agcctgcgat taaggtaaag 1920

cagcagtgca gttacaaacc taaacaaaca agagtcagcg aactgaaaag aagaccggga 1980

agaagaaggg aaaaaaaaaa gacccgaaca gagttttgat gagcagtgtc aggtctgtca 2040

catgagatga gagcgaagta gctcagctca gctcagcccc cactcgctca catggaacgc 2100

ctctgctccc cgcgactact taaccagcta aacgctcggt tgattaggag agaagaaaaa 2160

aacgagggaa aaaacggtgg aaacacacgc aaaacacaca acgccgtgag gccttgtaaa 2220

tacgggcgac gccgacgcgc atcgctaccc gaacaccaaa cgcctcagct tgccttggct 2280

ctcgcgagtc gctacgcccg cgacgacacg gccgcgccgc gcgcgcgcga gcgagcgagc 2340

atgtcgggct cctctgctgc cgacccctcg ccatccgcgt cgaccgcggg ggcggcggcc 2400

tcgccgctcg cgctgctccg cgcgcacggg cacgggcacc tcacgccgcc gtcggcggcg 2460

acggggccgg cgccgccgcc gccgtcgccg gcgtcggggt cggcgccgcg ggactaccgc 2520

aaggggaact ggacgctcca cgagacgctc atcctcatca ccgccaagcg tctcgacgac 2580

gaccgccgcg ccggcgttgg gggtggcgcg gcggctggtg gcggtggcgc cggatcgccg 2640

ccgacgccga ggtcggcgga gcagcggtgg aagtgggtgg agaactactg ctggaagaac 2700

ggctgcctcc gcagccagaa ccagtgcaat gacaagtggg acaacctcct ccgcgactac 2760

aagaaggtcc gcgactacga gtcccgcgtc gccgccgccg ccgtcaccgg cggcgcggcc 2820

gccgccaact ccgcccccct cccgtcgtac tggacgatgg agcggcacga gcgcaaggac 2880

tgcaacctcc ccaccaacct ggcgccggag gtctacgacg cgctctccga ggtgctctcc 2940

cgccgcgcgg cgcgacgcgg cggcgccacg atcgcgccca ccccgccgcc accaccgctc 3000

gcgctgccgc cgccgccgcc gccgccctcg ccgccgaagc ctctcgtcgc gcagcagcac 3060

caccaccatc acggccatca ccaccaccca cctcctcctc agccgccgcc gtcgtcgctg 3120

cagctccctc cggcggtcgt ggctccgccg ccggcgtccg tttccggtaa tggtcggtgc 3180

gcgcaccgta cacttaatac tcgtagtagc tgttacatcc cctcccctcc aaaccattta 3240

ctactgttct ctcacactga catgtggggc ccacctcgca gcgagctgag ctccgccact 3300

atacgttatt aaaagcccgc gttatgattg ggctagttac gttgttgagt tgagctggtc 3360

gtaattattt actaccgcta cttttttttt acctttttac cgtggggttc gggagagggt 3420

ggtcgcggta ataataatgt cctcaactca ggggttggga gaataaagct gcgtgcagtg 3480

tggtgcagtt catgcatggg aaaggtgatg cgaatccgga tattttatgg gggtttaatt 3540

gaaagattta ctccacgacg atactaccct gtactcctgc catgctgcaa gcatgcgtaa 3600

tgcgttacat tgcgaaatca ctcgctttga aagaaaaaaa agcctaaaac tttggagcaa 3660

aaaaaaagca ccttttgttt tctcctcgtg catgcatgcc gcgctgccta tcttgaacta 3720

ctttggactt ttgtatcgat cagcaaaact atacctatat tagcagtaat taatactaca 3780

tttgtagata tcctttgact gttctatctt atttttgata attaaaaaaa ttagttacat 3840

ttaaaaatgc tatttatatt ttatcatcta ataacaataa aagtattagt taaatattaa 3900

acgttggata tgaatagttt aaaactgtat tgttttgggg cggagggagt aattgattga 3960

tggattaatt aagtggttga ctaatgtgtg tgtgatttat ttgtgtagcg gaggaggaga 4020

tgtcggggtc gtcggagtcg ggggaggagg aggaggggtc gggcggggag ccggaggcga 4080

agcggcggcg gctgagccgg ctggggtcga gcgtggtgag gagcgcgacg gtggtggcga 4140

ggacgctggt ggcgtgcgag gagaagcggg agcgccggca ccgggagctg ctgcagctgg 4200

aggagcggcg gctgcgcctc gaggaggagc gcaccgaggt ccgccgccag ggcttcgccg 4260

gcctcatcgc cgccgtcaac agcctctcct ccgccatcca cgccctcgtc tccgaccacc 4320

gcagcggcga ctcctccggc cgatgatcgc cattgcaatc atatgcaatg cagcaaggac 4380

gatcgatgta aataacccat ggagatgcat ggatcgaggc atcggattat tatgtttgga 4440

atggctgcaa gaagaggagt agctaggcta ggggattaat tttttttttt ttgagtgtgc 4500

atcgccatcg cgtcgtctgc gagtattggg agtacggtgc attgcatgca caacgcctcc 4560

gtttcttgat ttctttcttt ctctcctgtg tcctgtgatt tttttgttgt ttattctttt 4620

cgtgcaatta gtggagagac tggcaggtgt gtggtgtgaa tgatcgaaat ggttagtgtg 4680

gctgctgctg gtggtgctgt tgctgttgct gcgttttttc ttctcgggtg ttgggtttgt 4740

cgtggacggc gatcattagc ggcacagtgg atgagagagc tgagctctag ctgcaggtcg 4800

cttggtctcg ctgcgctgtc atgtgatcac tactgcgcat gggggccacg gttaccgcta 4860

tagctgctgc aactgcgtgc gagaacgagc tcgtgctgag ctatacaagt ggcactggcc 4920

gtcgttttac a 4931

<210> 4

<211> 4893

<212> DNA

<213>African rice

<400> 4

gctggccgta gaagtcaagc ccatctcaca gtctgcagat cgcttctttt tacaacccaa 60

cgggccattt tcagaggcgt cacccgtaaa tccttgggcc aatgtgaccc aatcaatttc 120

gtgaaaagta ctccgtagca aacaacgcgc gcgcgttccc tgcaaaaaac gccggaaaac 180

gcattgccga gcgagccgtt tcagcaaata agggatcgtt agactttatc agtggctgta 240

tgaaactttt atatatgtgt gtgttcttag gacttaaaag ataatgctga aaaatttatt 300

tcaaaattta aattttggtt ttgacttatt ataaattttt ggggcaaccg atggggctat 360

gaggctatga gctatccatc catccatagc gcaacgtgtg acagcgtgag gtgacaagat 420

ggaaaaccgt gagccgcgcc accggtgtaa accccctccc ttcacgcgcg tttactttgc 480

aagcaaagga caaggaaaaa agccgtaatt ggcgaagaat gttcttggat ttgattagca 540

gcggtacggt aacacctcta gcttgcattt gcatggtagg gtgatttggc taggagagca 600

ggatcatcca cgcattcaat tttcctcatt tctttttgca ggctcaatag aggagaggag 660

gggcctagct agcaccaaga taatgcagca aaaaggagag aagagaacat gtcatccagt 720

ccaaagtgca aacgtctcta tacaaatgga acatatggag tggacctaag cctagttgct 780

tctgtgtccc aagctcccaa gtgtcaaagc cttaatgtgc aaaaccacat ctgtggggac 840

tcctgaaagc aaactaaatg attgcaaaag agttcgttta ttcacctatt tatttattta 900

ttttattttc tttccatgtg tctgttgttt gatactttga ttgatcatgt gctaaactgc 960

agaaaaaacc aacaggccca gagtaccggc gaaaaggctc ctcgatgaaa agtgcaggga 1020

ttctggggga gttgccagtg cgccaccgtt ggatttttgc acacatataa acagggctct 1080

gctgcacctc tcacatagta acatatatag ggacggttcc accaagcacg tttgggaaat 1140

ggttttttga gcaacatctc atgcgaacaa tttaggcact atttacttgt accttcacct 1200

tgtacgtgtg ctccatccca tagctgcatt atttgcataa atccttttaa ttgcatatat 1260

acatactctc tccatatttt ttttaataaa tggcgtattt cactttttat cacaccatgt 1320

cttttaataa gacgaaatat caaatgtgtg acaaaaaaat caataatgtc atctattaaa 1380

agacagatta agtatatgag aggcatttaa ttatttgcca cctttgatgt tttacaattt 1440

atcactcgtg tgacatagac gcatagggta acaaattgcg aaacatcaag taatctgtga 1500

gtagcaaata cttaaatatc tcctaatatg ttagcattgc acccaacttt atatcaaaac 1560

tctataacta tattgagctt tgtcctgccc atggtaaagt gtggggttaa agtgttaaaa 1620

agctattggt agcagtatct agtatggcgt ggcaggccgg tgcagataaa aggggccccg 1680

ggttttgccc ggaaatgagg agaggacgcc ccggcagcta gcggacgctt tgcatttgca 1740

tgcatggtgc tctctacttc tccctccgtt cccacggcaa atgcaaaacg catgctagta 1800

tttggcacca ttagcctaag atagtagtat cagagtgtta cagaaagaat ataattatgt 1860

agtagtatag taattaatta aagcctgcga ttaaggtaaa gcagcagtgc agttacaaac 1920

ctaaacaaac aagagtcagc gaactgaaaa gaagaccggg aagaagaagg gaaaaaaaaa 1980

agacccgaac agagttttga tgagcagtgt caggtctgtc acatgagatg agagcgaagt 2040

agctcagctc agctcagccc ccactcgctc acatggaacg cctctgctcc ccgcgactac 2100

ttaaccagct aaacgctcgg ttgattagga gagaagaaaa aaacgaggga aaaaacggtg 2160

gaaacacacg caaaacacac aacgccgtga ggccttgtaa atacgggcga cgccgacgcg 2220

catcgctacc cgaacaccaa acgcctcagc ttgccttggc tctcgcgagt cgctacgccc 2280

gcgacgacac ggccgcgccg cgcgcgcgcg agcgagcgag catgtcgggc tcctctgctg 2340

ccgacccctc gccatccgcg tcgaccgcgg gggcggcggc ctcgccgctc gcgctgctcc 2400

gcgcgcacgg gcacgggcac ctcacgccgc cgtcggcggc gacggggccg gcgccgccgc 2460

cgccgtcgcc ggcgtcgggg tcggcgccgc gggactaccg caaggggaac tggacgctcc 2520

acgagacgct catcctcatc accgccaagc gtctcgacga cgaccgccgc gccggcgttg 2580

ggggtggcgc ggcggctggt ggcggtggcg ccgggtcgcc gccgacgccg aggtcggcgg 2640

agcagcggtg gaagtgggtg gagaactact gctggaagaa cggctgcctc cgcagccaga 2700

accagtgcaa tgacaagtgg gacaacctcc tccgcgacta caagaaggtc cgcgactacg 2760

agtcccgcgt cgccgccgcc gccgtcaccg gcggcgcggc cgccgccaac tccgcccccc 2820

tcccgtcgta ctggacgatg gagcggcacg agcgcaagga ctgcaacctc cccaccaacc 2880

tggcgccgga ggtctacgac gcgctctccg aggtgctctc ccgccgcgcg gcgcgacgcg 2940

gcggcgccac gatcgcgccc accccgccgc caccaccgct cgcgctgccg ccgccgccgc 3000

cgccgccgcc gccctcgccg ccgaagcctc tcgtcgcgca gcagcaccac caccatcacg 3060

gccatcacca ccacccacct cctcctcagc cgccgccgtc gtcgctgcag ctccctccgg 3120

cggtcgtggc tccgccgccg gcgtccgttt ccggtaatgg tcggtgcgcg caccgtacac 3180

ttaatactcg tagtagctgt tacatcccct cccctccaaa ccatttacta ctgttctctc 3240

acactgacat gtggggccca cctcgcagcg agctgagctc cgccactata cgttattaaa 3300

agcccgcgtt atgattgggc tagttacgtt gttgagttga gctggtcgta attatttact 3360

accgctactt tttttttacc tttttaccgt ggggttcggg agagggtggt cgcggtaata 3420

ataatgtcct caactcaggg gttgggagaa taaagctgcg tgcagtgtgg tgcagttcat 3480

gcatgggaaa ggtgatgcga atccggatat tttatggggg tttaattgaa agatttactc 3540

cacgacgata ctaccctgta ctcctgccat gctgcaagca tgcgtaatgc gttacattgc 3600

gaaatcactc gctttgaaag aaaaaaaagc ctaaaacttt ggagcaaaaa aaaagcacct 3660

tttgttttct cctcgtgcat gcatgccgcg ctgcctatct tgaactactt tggacttttg 3720

tatcgatcag caaaactata cctatattag cagtaattaa tactacattt gtagatatcc 3780

tttgaccgtt ctatcttatt tttgataatt aaaaaaatta gttacattta aaaatactat 3840

ttatatttta tcatctaata acaataaaag tattagttaa atattaaacg ttggatatga 3900

atagtttaaa actgtattgt tttggggcgg agggagtaat tgattgatgg attaattaag 3960

tggttgacta atgtgtgtgt gatttatttg tgtagcggag gaggagatgt cggggtcgtc 4020

ggagtcgggg gaggaggagg aggggtcggg cggggagccg gaggcgaagc ggcggcggct 4080

gagccggctg gggtcgagcg tggtgaggag cgcgacggtg gtggcgagga cgctggtggc 4140

gtgcgaggag aagcgggagc gccggcaccg ggagctgctg cagctggagg agcggcggct 4200

gcgcctcgag gaggagcgca ccgaggtccg ccgccagggc ttcgccggcc tcatcgccgc 4260

cgtcaacagc ctctcctccg ccatccacgc cctcgtctcc gaccaccgca gcggcgactc 4320

ctccggccga tgatcgccat tgcaatcata tgcaatgcag caaggacgat cgatgtaaat 4380

aacccatgga gatgcatgga tcgaggcatc ggattattat gtttggaatg gctgcaagaa 4440

gaggagtagc taggctaggg gattaatttt tttttttttg agtgtgcatc gccatcgcgt 4500

cgtctgcgag tattgggagt acggtgcatt gcatgcacaa cgcctccgtt tcttgatttc 4560

tttctttctc tcctgtgtcc tgtgattttt ttgttgttta ttcttttcgt gcaattagtg 4620

gagagactgg caggtgtgtg gtgtgaatga tcgaaatggt tagtgtggct gctgctggtg 4680

gtgctgttgc tgttgctgcg ttttttcttc tcgggtgttg ggtttgtcgt ggacggcgat 4740

cattagcggc acagtggatg agagagctga gctctagctg caggtcgctt ggtctcgctg 4800

cgctgtcatg tgatcactac tgcgcatggg ggccacggtt accgctatag ctgctgcaac 4860

tgcgtgcgag aacgagctcg tgctgagcta tac 4893

<210> 5

<211> 1167

<212> DNA

<213>Artificial sequence

<220>

<223>

<400> 5

atgtcgggct cctctgctgc cgacccctcg ccatccgcgt cgaccgcggg ggcggcggcc 60

tcgccgctcg cgctgctccg cgcgcacggg cacgggcacc tcacgccgcc gtcggcggcg 120

acggggccgg cgccgccgcc gccgtcgccg gcgtcggggt cggcgccgcg ggactaccgc 180

aaggggaact ggacgctcca cgagacgctc atcctcatca ccgccaagcg tctcgacgac 240

gaccgccgcg ccggcgttgg gggtggcgcg gcggctggtg gcggtggcgc cggatcgccg 300

ccgacgccga ggtcggcgga gcagcggtgg aagtgggtgg agaactactg ctggaagaac 360

ggctgcctcc gcagccagaa ccagtgcaat gacaagtggg acaacctcct ccgcgactac 420

aagaaggtcc gcgactacga gtcccgcgtc gccgccgccg ccgtcaccgg cggcgcggcc 480

gccgccaact ccgcccccct cccgtcgtac tggacgatgg agcggcacga gcgcaaggac 540

tgcaacctcc ccaccaacct ggcgccggag gtctacgacg cgctctccga ggtgctctcc 600

cgccgcgcgg cgcgacgcgg cggcgccacg atcgcgccca ccccgccgcc accaccgctc 660

gcgctgccgc cgccgccgcc gccgccctcg ccgccgaagc ctctcgtcgc gcagcagcac 720

caccaccatc acggccatca ccaccaccca cctcctcctc agccgccgcc gtcgtcgctg 780

cagctccctc cggcggtcgt ggctccgccg ccggcgtccg tttccggtgc ggaggaggag 840

atgtcggggt cgtcggagtc gggggaggag gaggaggggt cgggcgggga gccggaggcg 900

aagcggcggc ggctgagccg gctggggtcg agcgtggtga ggagcgcgac ggtggtggcg 960

aggacgctgg tggcgtgcga ggagaagcgg gagcgccggc accgggagct gctgcagctg 1020

gaggagcggc ggctgcgcct cgaggaggag cgcaccgagg tccgccgcca gggcttcgcc 1080

ggcctcatcg ccgccgtcaa cagcctctcc tccgccatcc acgccctcgt ctccgaccac 1140

cgcagcggcg actcctccgg ccgatga 1167

<210> 6

<211> 1173

<212> DNA

<213>Artificial sequence

<400> 6

atgtcgggct cctctgctgc cgacccctcg ccatccgcgt cgaccgcggg ggcggcggcc 60

tcgccgctcg cgctgctccg cgcgcacggg cacgggcacc tcacgccgcc gtcggcggcg 120

acggggccgg cgccgccgcc gccgtcgccg gcgtcggggt cggcgccgcg ggactaccgc 180

aaggggaact ggacgctcca cgagacgctc atcctcatca ccgccaagcg tctcgacgac 240

gaccgccgcg ccggcgttgg gggtggcgcg gcggctggtg gcggtggcgc cgggtcgccg 300

ccgacgccga ggtcggcgga gcagcggtgg aagtgggtgg agaactactg ctggaagaac 360

ggctgcctcc gcagccagaa ccagtgcaat gacaagtggg acaacctcct ccgcgactac 420

aagaaggtcc gcgactacga gtcccgcgtc gccgccgccg ccgtcaccgg cggcgcggcc 480

gccgccaact ccgcccccct cccgtcgtac tggacgatgg agcggcacga gcgcaaggac 540

tgcaacctcc ccaccaacct ggcgccggag gtctacgacg cgctctccga ggtgctctcc 600

cgccgcgcgg cgcgacgcgg cggcgccacg atcgcgccca ccccgccgcc accaccgctc 660

gcgctgccgc cgccgccgcc gccgccgccg ccctcgccgc cgaagcctct cgtcgcgcag 720

cagcaccacc accatcacgg ccatcaccac cacccacctc ctcctcagcc gccgccgtcg 780

tcgctgcagc tccctccggc ggtcgtggct ccgccgccgg cgtccgtttc cggtgcggag 840

gaggagatgt cggggtcgtc ggagtcgggg gaggaggagg aggggtcggg cggggagccg 900

gaggcgaagc ggcggcggct gagccggctg gggtcgagcg tggtgaggag cgcgacggtg 960

gtggcgagga cgctggtggc gtgcgaggag aagcgggagc gccggcaccg ggagctgctg 1020

cagctggagg agcggcggct gcgcctcgag gaggagcgca ccgaggtccg ccgccagggc 1080

ttcgccggcc tcatcgccgc cgtcaacagc ctctcctccg ccatccacgc cctcgtctcc 1140

gaccaccgca gcggcgactc ctccggccga tga 1173

Claims (10)

1. protein, is following any：

A) protein being made up of the amino acid sequence shown in sequence in sequence table 1；

B) protein being made up of the amino acid sequence shown in sequence in sequence table 2；

C) substitution and/or missing by amino acid sequence a) or b) limited by one or several amino acid residues and/or Addition, and the protein related to the grain length and/or seed holding of vegetable seeds；

D) and a)-c) in any limited amino acid sequence have more than 99%, more than 95%, more than 90%, more than 85% Or more than 80% homology, and the protein related to the grain length and/or seed holding of vegetable seeds.

2. encode the nucleic acid molecules of protein described in claim 1.

3. nucleic acid molecules according to claim 2, it is characterised in that：The nucleic acid molecules are described in coding claim 1 The gene of protein, the gene is following any DNA molecular：

1) DNA molecular in sequence table shown in 2341-4346 of sequence 3；

2) DNA molecular in sequence table shown in 2322-4333 of sequence 4；

3) DNA molecular in sequence table shown in sequence 5；

4) DNA molecular in sequence table shown in sequence 6；

5) under strict conditions with 1) -4) in any restriction DNA molecular hybridization and coding claim 1 described in protein DNA molecular；

6) with 1) -5) in any restriction DNA sequence dna have more than 99%, more than 95%, more than 90%, more than 85% or More than 80% homology, and protein DNA molecule described in coding claim 1.

4. recombinant vector, expression cassette, transgenic cell line or recombinant bacterium containing nucleic acid molecules described in Claims 2 or 3.

5. recombinant vector according to claim 4, it is characterised in that：The recombinant vector is recombinant expression carrier or restructuring Cloning vector.

6. nucleic acid molecules described in protein described in claim 1 or Claims 2 or 3 or the restructuring described in claim 4 or 5 are carried Body, expression cassette, transgenic cell line or recombinant bacterium it is following it is any in application：

(a) the seed length of vegetable seeds is increased；

(b) the increased plant variety of seed selection seed length；

(c) seed holding of vegetable seeds is improved；

(d) plant variety that seed selection seed holding is improved.

7. cultivating the method for the plant that the increase of seed length and/or seed holding are improved, comprise the following steps：Improve in recipient plant Protein expression amount and/or activity described in claim 1, so as to obtain the plant that the increase of seed length and/or seed holding are improved Thing.

8. the method with the genetically modified plants of character shown in following (b1) and/or (b2) is cultivated, including it is following (a1) and (a2) The step of：

(a1) encoding gene of protein described in claim 1 is imported into recipient plant, obtains expressing the encoding gene Genetically modified plants；

(a2) obtained in genetically modified plants compared with the recipient plant obtained by the step (a1), with following (b1) and/or (b2) genetically modified plants of character shown in：

(b1) seed length increase；

(b2) seed holding is improved.

9. according to any described application or method in claim 6-8, it is characterised in that：The plant is monocotyledon.

10. application according to claim 9 or method, it is characterised in that：The monocotyledon is grass；

Specifically, the grass is paddy rice.