CN106926334B - It is a kind of to be used for microbial immobilized natural wooden fiber's carrier and preparation method thereof - Google Patents

It is a kind of to be used for microbial immobilized natural wooden fiber's carrier and preparation method thereof Download PDF

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CN106926334B
CN106926334B CN201710124283.4A CN201710124283A CN106926334B CN 106926334 B CN106926334 B CN 106926334B CN 201710124283 A CN201710124283 A CN 201710124283A CN 106926334 B CN106926334 B CN 106926334B
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wooden fiber
natural wooden
carrier
reaction kettle
pressure
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CN106926334A (en
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赵岩
徐颖杰
刘珊
谭豪波
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Beijing Normal University
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Beijing Normal University
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
    • B27K—PROCESSES, APPARATUS OR SELECTION OF SUBSTANCES FOR IMPREGNATING, STAINING, DYEING, BLEACHING OF WOOD OR SIMILAR MATERIALS, OR TREATING OF WOOD OR SIMILAR MATERIALS WITH PERMEANT LIQUIDS, NOT OTHERWISE PROVIDED FOR; CHEMICAL OR PHYSICAL TREATMENT OF CORK, CANE, REED, STRAW OR SIMILAR MATERIALS
    • B27K5/00—Treating of wood not provided for in groups B27K1/00, B27K3/00
    • B27K5/0085—Thermal treatments, i.e. involving chemical modification of wood at temperatures well over 100°C
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Thermal Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Forests & Forestry (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

The present invention relates to field of microbial fermentation, and in particular to a kind of to be used for microbial immobilized natural wooden fiber's carrier and preparation method thereof.This method comprises the following steps：1) the relatively uniform natural wooden fiber's material of material is chosen, and mechanical pretreatment is carried out to it；2) water is added into high-temperature high-pressure reaction kettle, then adds natural wooden fiber's material after step 1) processing；3) natural wooden fiber's material in step 2) and water are forced into assigned temperature and specified pressure to carry out hydro-thermal reaction jointly, and continue hydro-thermal reaction to specified time；4) after the hydro-thermal reaction of step 3), the temperature and pressure in high-temperature high-pressure reaction kettle is reduced, and take out natural wooden fiber's material after hydro-thermal process；5) natural wooden fiber's material obtained by step 4) is cleaned into drying.The present invention improves microorganism fixed effect, significant in microbial immobilized, has a extensive future.

Description

It is a kind of to be used for microbial immobilized natural wooden fiber's carrier and preparation method thereof
Technical field
The present invention relates to technical field of microbial fermentation, is used for microbial immobilized natural wood more particularly to a kind of Fiber carrier and preparation method thereof.
Background technology
In the various techniques based on microbial fermentation, the microbial reaction of free state is relatively inefficient, and is vulnerable to The suppression of harmful substance in matrix, becomes and restricts one of key factor that technique is promoted often.And microbial immobilized is to improve Reaction system biomass, the common method for protecting microorganism to be impacted from harmful constituent, and it is easier to product separation and technique control System, is widely used to all kinds of microorganism fermentation process.Most common process for fixation is calcium alginate isogel fixation, and Gel carrier is in CO2In the presence of it is unstable, be restricted its application in microbial ethanol fermentation etc. in related process.Micro- life Thing field recent studies suggest that, microbial cell can be adsorbed and fixed on a variety of natural carrier surfaces.With manually closing Compared into carrier, using the natural wooden fiber such as bagasse, rice husk, chalina carrier fixation of microbe cell, have cost it is low, Intensity height, nonhazardous, can reuse and a variety of advantages such as porosity is big.For example, Sittijunda etc. utilizes the carriers such as vegetable seed stalk Fixed Thermophilic Bacterium carries out the alcohol fermentation of glucose/xylose, finds compared with free microorganism, and vegetable seed stalk, which is fixed, to be had Effect improves alcohol yied and concentration.
Live to further improve metabolism of the natural carrier to microbial immobilization effect, reinforcing immobilized microorganism Property, the researcher of association area proposes and artificial modification carried out to natural carrier, optimizes the physicochemical property and structure of natural carrier. Some researches show that by the use of cellulase corn straw modification as microbe carrier, can destroy the smooth surface of stalk, increase Adding carrier roughness and porosity, so as to more effectively improve the biomass of fixed saccharomyces cerevisiae.Park etc. is simulated for timber Gel as enzyme immobilization carrier research find, by varying lignin, hemicellulose and content of cellulose in carrier with than Example, can adjust the physicochemical properties such as the hydrophobicity of carrier, porosity, dielectric constant, so as to improve immobilization effect.Can To see, the immobilization technology using natural wooden fiber's carrier is the important channel for breaking through various microbial fermentation technology bottlenecks, and Modification to carrier is to improve the key of biological fixnig effect.
The wood fibre waste such as agricultural crop straw is both typical agricultural solid waste, and the biology of great exploitation potential Matter resource.For the special construction and property of the wood fibre waste such as stalk, the present invention proposes one kind and is based on digestion work The wood fibre support modification technology of skill technology and the preparation method of respective carrier, using wood fibre waste in different temperatures and Under the hydrothermal reaction condition of different residence times, the component such as its lignin, hemicellulose and cellulose can produce in various degree The characteristic of dissolving, realizes the modification of undissolved solid-state carrier, with adjust the specific surface area of natural wooden fiber's carrier, porosity and The physicochemical properties such as aperture, improve carrier to microbial immobilization effect.
The content of the invention
(1) technical problems to be solved
The technical problem to be solved in the present invention is to provide it is a kind of for microbial immobilized natural wooden fiber's carrier and Its preparation method, can utilize the characteristics such as natural carrier cost low, intensity is high, nonhazardous and pass through hydrothermal modification adjustment hole The physical and chemical parameter such as porosity and specific surface area, it is intended to which natural carrier is microbial immobilized in raising and improvement microorganism fermentation process Effect.
(2) technical solution
In order to solve the above technical problem, the present invention provides a kind of for microbial immobilized natural wooden fiber's load The preparation method of body, it comprises the following steps：
1) the relatively uniform natural wooden fiber's material of material is chosen, and mechanical pretreatment is carried out to it；
2) water is added into high-temperature high-pressure reaction kettle, then adds natural wooden fiber's material after step 1) processing；
3) by natural wooden fiber's material in step 2) and water it is common be forced into assigned temperature and specified pressure with Hydro-thermal reaction is carried out, and continues the hydro-thermal reaction to specified time；
4) after the hydro-thermal reaction described in step 3), the temperature and pressure in the high-temperature high-pressure reaction kettle is reduced, and Take out natural wooden fiber's material after hydro-thermal process；
5) natural wooden fiber's material obtained by step 4) is cleaned into drying, to obtain the final product.
Preferably, in step 2), the cumulative volume of the natural wooden fiber's material added into the high-temperature high-pressure reaction kettle No more than the 1/2 of high-temperature high-pressure reaction kettle dischargeable capacity, the natural wooden fiber's material and the cumulative volume of water that are added are high temperature The 1/3~2/3 of autoclave dischargeable capacity.
Preferably, the high-temperature high-pressure reaction kettle can be standardized product or customed product, its operating temperature section for 160~ 250 DEG C, operation pressure interval is 3~6MPa, its dischargeable capacity is determined according to the amount of prepared destination carrier.
Wherein, step 1) natural wooden fiber's material is woody or gramineae plant stem or wood fibre waste； Preferably, the xylophyta is willow or pine tree, and the gramineae plant is corn.
Preferably, the wood fibre waste is hardwood or vegetable cork.
Wherein, the step 1) mechanical pretreatment is：It is 0.5~4cm of the length of side by natural wooden fiber's material cutting 1~4cm of cube or diameter, height 1~4cm cylinder, cleaning, drying, you can.
Wherein, the step 3) assigned temperature, specified pressure and specified time are respectively 160~250 DEG C, 3~6MPa and 5 ~60min.
Wherein, the step 5) drying is that 24h is dried at 60~80 DEG C with up to constant weight.
Further, the present invention provides a kind of preferred solution of the method, it comprises the following steps：
1) the relatively uniform natural wooden fiber's material of material is chosen, is cut to the cube or straight of 1~3cm of the length of side The cylinder of 1~3cm of footpath, 1~3cm of height, are cleaned with deionized water and drying to constant weight at 60~80 DEG C；
2) water (preferably distilled water or deionized water) is added into high-temperature high-pressure reaction kettle, then adds and is handled through step 1) Natural wooden fiber's material afterwards, the cumulative volume of the natural wooden fiber's material added into the high-temperature high-pressure reaction kettle is height The 1/3 of warm autoclave dischargeable capacity, the natural wooden fiber's material and the cumulative volume of water added are high-temperature high-voltage reaction The 1/2 of kettle dischargeable capacity；
3) by natural wooden fiber's material in step 2) and water it is common be forced into 200~210 DEG C and 4~5MPa with Hydro-thermal reaction is carried out, and continues the 20~30min of hydro-thermal reaction；
4) after the hydro-thermal reaction described in step 3), the temperature in the high-temperature high-pressure reaction kettle is reduced by water-bath cooling Degree and pressure, to normal temperature and pressure after open the high-temperature high-pressure reaction kettle, take out natural wooden fiber's material；
5) natural wooden fiber's material obtained by step 4) is washed with deionized water only, and 24h is dried at 60~80 DEG C With up to constant weight, up to natural wooden fiber's carrier after hydrothermal modification.
Present invention also offers natural wooden fiber's carrier prepared by a kind of any of the above-described method.Before the hydrothermal modification The physicochemical property of natural wooden fiber's carrier afterwards, can carry out analysis measure by parameters such as porosity, aperture and specific surface areas.
Present invention also offers application of the above-mentioned natural wooden fiber's carrier in microbial immobilized, the microorganism are excellent Elect brewer's yeast as.
(3) beneficial effect
The above-mentioned technical proposal of the present invention has the advantages that：
1) preparation method of the present invention uses the hardwood or vegetable cork of the xylophyta such as axis such as poplar, pine, and Crop material, plug or the wood fibre waste of gramineae plant such as corn etc. are as support material, for the money of waste wood fiber Sourceization utilizes and provides new way.
2) the problems such as power is weaker, vector stabilisation is poor is combined between traditional absorption carrier and cell, the present invention passes through The modification that different hydrothermal conditions realize carrier is provided, can adjust and optimize the parameters such as carrier porosity, aperture and specific surface area, To obtain the hydrothermal modification carrier strong with objective microbe combination power, immobilization effect is good.
3) can be that microorganism growth carries without adding other artificial materials using modified natural wooden fiber's carrier For certain energy substance, and it is avoided that toxic action or adverse effect of the artificial material to microorganism.
4) preparation method of natural wooden fiber's carrier is easy to operate, technical application, of low cost, modified and prepare bar Part is easily controllable, modified support product to microorganism nonhazardous, using it is convenient, mechanical strength is suitable and can adjust as needed Whole and optimization.
Therefore, natural wooden fiber's carrier provided by the invention and preparation method thereof, can utilize natural carrier cost it is low, The characteristics such as intensity height, nonhazardous, and can improve and improve by physical and chemical parameters such as hydrothermal modification adjustment hole porosity and specific surface areas The microbial immobilized effect of natural carrier in microorganism fermentation process, in the polytechnic microorganism such as fermentation, water process Important function has been played in immobilization.The present invention prepares microbial immobilized load using hydro-thermal method modified natural wood fibre waste Body, is not only able to change wood fibre loose structure and makes it easier for microorganism growth, and natural carrier can be utilized to adsorb Property buffering harmful substance inhibitory action, have broad prospect of application in fields such as microbial fermentations.
Embodiment
Embodiments of the present invention are described in further detail with reference to embodiment.Following embodiments are used to illustrate this Invention, but cannot be used for limiting the scope of the invention.
Embodiment 1
A kind of preparation method for the natural wooden fiber's carrier for being used to fix brewer's yeast, it is adopted by matrix of maize straw It is prepared with method comprising the following steps：
1) choose the relatively uniform maize straw of material, be cut to the cube or diameter 1cm height 1cm of length of side 1cm Cylinder, is cleaned with deionized water and 24h is dried at 75 DEG C with up to constant weight.
2) deionized water is added into customization 600mL high-temperature high-pressure reaction kettles, and adds the pretreated corn of step 1) 20~30 groups of stalk, the cumulative volume of the maize straw of addition are the 1/3 of high-temperature high-pressure reaction kettle dischargeable capacity, the corn stalk of addition The cumulative volume of stalk and water is the 1/2 of high-temperature high-pressure reaction kettle dischargeable capacity.
3) hydrothermal reaction condition is provided for above-mentioned high-temperature high-pressure reaction kettle, specific assigned temperature is 200 DEG C, specified pressure For 4.5MPa, maize straw therein and water are forced into the condition, sustained response to specified time 20min jointly.
4) after reaching the above-mentioned 20min specified, the temperature of above-mentioned high-temperature high-pressure reaction kettle is reduced rapidly by water-bath cooling And pressure, to normal temperature and pressure after open high-temperature high-pressure reaction kettle, take out the maize straw after hydro-thermal process.
5) treated maize straw is washed with deionized water only, and dries at 75 DEG C 24h with up to constant weight, i.e., The maize straw carrier after hydrothermal modification is obtained, is used in case follow-up brewer's yeast etc. is microbial immobilized.
Embodiment 2
A kind of preparation method for the natural wooden fiber's carrier for being used to fix brewer's yeast, it is adopted by matrix of maize straw It is prepared with method comprising the following steps：
1) choose the relatively uniform maize straw of material, be cut to the cube or diameter 2cm height 2cm of length of side 2cm Cylinder, is cleaned with deionized water and 24h is dried at 75 DEG C with up to constant weight.
2) deionized water is added into customization 600mL high-temperature high-pressure reaction kettles, and adds the pretreated corn of step 1) 20~30 groups of stalk, the cumulative volume of the maize straw of addition are the 1/3 of high-temperature high-pressure reaction kettle dischargeable capacity, the corn stalk of addition The cumulative volume of stalk and water is the 1/2 of high-temperature high-pressure reaction kettle dischargeable capacity.
3) hydrothermal reaction condition is provided for above-mentioned high-temperature high-pressure reaction kettle, specific assigned temperature is 210 DEG C, specified pressure For 4.5MPa, maize straw therein and water are forced into the condition, sustained response to specified time 20min jointly.
4) after reaching the above-mentioned 20min specified, the temperature of above-mentioned high-temperature high-pressure reaction kettle is reduced rapidly by water-bath cooling And pressure, to normal temperature and pressure after open high-temperature high-pressure reaction kettle, take out the pine waste material after hydro-thermal process.
5) treated pine waste material is washed with deionized water only, and dries at 75 DEG C 24h with up to constant weight, i.e., The pine carrier after hydrothermal modification is obtained, is used in case follow-up brewer's yeast etc. is microbial immobilized.
Comparative example 1
A kind of preparation method (without hydrothermal modification) for the natural wooden fiber's carrier for being used to fix brewer's yeast, it is with jade Rice stalk is prepared for matrix：
The relatively uniform maize straw of material is chosen, is cut to the cube of length of side 1cm or the circle of diameter 1cm height 1cm Cylinder, is cleaned with deionized water and 24h is dried at 75 DEG C with up to constant weight.The maize straw without hydrothermal modification is obtained to carry Body, is used in case follow-up brewer's yeast etc. is microbial immobilized.
Comparative example 2
A kind of preparation method for the natural wooden fiber's carrier for being used to fix brewer's yeast, it is adopted by matrix of maize straw It is prepared with method comprising the following steps：
1) choose the relatively uniform maize straw of material, be cut to the cube or diameter 2cm height 2cm of length of side 2cm Cylinder, is cleaned with deionized water and 24h is dried at 75 DEG C with up to constant weight.
2) deionized water is added into customization 600mL high-temperature high-pressure reaction kettles, and adds the pretreated corn of step 1) 20~30 groups of stalk, the cumulative volume of the maize straw of addition are the 1/3 of high-temperature high-pressure reaction kettle dischargeable capacity, the corn stalk of addition The cumulative volume of stalk and water is the 1/2 of high-temperature high-pressure reaction kettle dischargeable capacity.
3) hydrothermal reaction condition is provided for above-mentioned high-temperature high-pressure reaction kettle, specific assigned temperature is 190 DEG C, specified pressure 4MPa, the condition, sustained response to specified time 20min are forced into by maize straw therein and water jointly.
4) after reaching the above-mentioned 20min specified, the temperature of above-mentioned high-temperature high-pressure reaction kettle is reduced rapidly by water-bath cooling And pressure, to normal temperature and pressure after open high-temperature high-pressure reaction kettle, take out the maize straw after hydro-thermal process.
5) treated maize straw is washed with deionized water only, and dries at 75 DEG C 24h with up to constant weight, i.e., The maize straw carrier after hydrothermal modification is obtained, is used in case follow-up brewer's yeast etc. is microbial immobilized.
The detection of hydrothermal modification maize straw carrier prepared by the test example embodiment of the present invention 1,2 and microbial immobilized Effect
The maize straw carrier prepared to embodiment 1,2 and comparative example 1,2 carries out specific surface area and porosity detects, and with Brewer's yeast (Saccharomycvrrces Cerevisiae) is immobilization objective microbe, detects above-mentioned maize straw respectively The microbial immobilized effect of carrier.Concretely comprise the following steps：
1) hydrothermal modification or unmodified maize straw carrier prepared by a certain amount of embodiment 1,2 and comparative example 1,2 is respectively chosen Sample, is cut to maximal side in below 0.5cm, in case specific surface area and lacunarity analysis use.
2) specific surface area lacunarity analysis instrument (ASIQMOOO-3) is utilized, the hydrothermal modification chosen by the detection of nitrogen method is beautiful Rice stalk vector sample, the results show：
Maize straw carrier specific surface area and pore volume described in comparative example 1 without hydrothermal modification are respectively 1.421m2/g And 0.005cm3/g；The specific surface area of hydrothermal modification maize straw carrier and pore volume are respectively 1.572m described in comparative example 22/g And 0.009cm3/ g, improves 0.1 times and 0.8 times, water than the maize straw carrier without hydrothermal modification prepared by comparative example 1 respectively Although heat modification significantly increases pore volume, but the improvement result for contrasting surface area is limited.
The specific surface area of hydrothermal modification maize straw carrier and pore volume are respectively up to 2.440m described in embodiment 12/ g and 0.011cm3/ g, improves 0.7 times and 1.2 times than the maize straw carrier without hydrothermal modification in comparative example 1 respectively；Embodiment The specific surface area and pore volume of the 2 hydrothermal modification maize straw carriers are respectively up to 2.873m2/ g and 0.011cm3/ g, respectively 1.0 times and 1.2 times are improved than the maize straw carrier without hydrothermal modification in comparative example 1；Show at the same time, in embodiment 2 Hydrothermal modification maize straw carrier, than the hydrothermal modification maize straw carrier in embodiment 1, in the case where pore volume is identical, Specific surface area further improves 18%.
It is indicated above that maize straw carrier prepared by hydrothermal modification method of the present invention have good pore structure and Performance, the immobilization particularly suitable for microbial cell.
3) weigh 6g glucose be placed in 250mL conical flasks, add 150mL deionized waters, and add 1.5g peptones, 0.75g yeast extracts, are configured to the dextrose broth 150mL that concentration is 40g/L；3 parts are configured in the same way, wherein 1st part uses as the carrier immobilized detection of hydrothermal modification maize straw prepared by embodiment 1, and the 2nd part 1 prepares as a comparison case Unmodified maize straw it is carrier immobilized detection use, the 3rd part is used as blank control.
4) into the 1st and the 2nd part of above-mentioned dextrose broth, the hydrothermal modification for being separately added into the preparation of embodiment 1 is beautiful Unmodified maize straw carrier prepared by rice stalk vector and comparative example 1, utilizes sealed membrane sealing taper at the same time with the 3rd part afterwards Bottleneck, is placed in high-pressure sterilizing pot, and sterilize 30min postcoolings at 120 DEG C.
5) the rejuvenation brewer's yeast of phase homogenous quantities is inoculated with into the dextrose broth after 3 parts of sterilizings (Saccharomyces Cerevisiae), while be placed in isothermal vibration incubator, (frequency of oscillation is vibrated under 25 DEG C of perseverances 120h 180rpm) is cultivated, brewer's yeast (Saccharomyces Cerevisiae) is fully bred, and in the 1st part and the 2nd part It is fixed in dextrose broth on above-mentioned hydrothermal modification and unmodified maize straw carrier.
6) by the inoculum density detection method such as spectrophotometry or colony counting method, 3 parts of liquid of glucose cultures are detected Brewer's yeast (Saccharomyces Cerevisiae) inoculum density in base, clear and definite 1st part and the 2nd part by blank control In dextrose broth, list of the hydrothermal modification maize straw carrier to brewer's yeast strain of the preparation of embodiment 1 is fixed on Position volume adsorption is up to 1.67 × 109/cm3, the unmodified maize straw carrier adsorption amount (1.12 × 10 of the preparation of comparative example 19/ cm3) 49% is improved, unit mass adsorbance improves 80%.
It is indicated above that maize straw carrier prepared by hydrothermal modification method of the present invention, effectively can adsorb and be enriched with The microorganism fungus kinds such as brewer's yeast (Saccharomyces Cerevisiae), realize good microbial immobilized effect.
The embodiment of the present invention provides for the sake of example and description, and is not exhaustively or by this to send out It is bright to be limited to disclosed form.Many modifications and variations are obvious for the ordinary skill in the art.Choosing It is to more preferably illustrate the principle of the present invention and practical application to select and describe embodiment, and makes those of ordinary skill in the art It will be appreciated that the present invention is so as to design the various embodiments with various modifications suitable for special-purpose.

Claims (4)

1. a kind of preparation method for microbial immobilized natural wooden fiber's carrier, it is characterised in that including following step Suddenly：
1) the relatively uniform natural wooden fiber's material of material is chosen, is cut to the cube or diameter 1 of 1~3cm of the length of side The cylinder of~3cm, 1~3cm of height, are cleaned with deionized water and drying to constant weight at 60~80 DEG C；
2) water is added into high-temperature high-pressure reaction kettle, natural wooden fiber's material after step 1) processing is then added, to institute The cumulative volume for stating the natural wooden fiber's material added in high-temperature high-pressure reaction kettle is the 1/ of high-temperature high-pressure reaction kettle dischargeable capacity 3, the natural wooden fiber's material and the cumulative volume of water added is the 1/2 of high-temperature high-pressure reaction kettle dischargeable capacity；
3) natural wooden fiber's material in step 2) and water are forced into 200~210 DEG C and 4~5MPa jointly to carry out Hydro-thermal reaction, and continue the 20~30min of hydro-thermal reaction；
4) after the hydro-thermal reaction described in step 3), reduce by water-bath cooling temperature in the high-temperature high-pressure reaction kettle and Pressure, to normal temperature and pressure after open the high-temperature high-pressure reaction kettle, take out natural wooden fiber's material；
5) natural wooden fiber's material obtained by step 4) is washed with deionized water only, and more than 24h is dried at 60~80 DEG C To constant weight, up to natural wooden fiber's carrier after hydrothermal modification.
A kind of 2. natural wooden fiber's carrier prepared by claim 1 the method.
3. application of natural wooden fiber's carrier in microbial immobilized described in claim 2.
4. application according to claim 3, it is characterised in that the microorganism is brewer's yeast.
CN201710124283.4A 2017-03-03 2017-03-03 It is a kind of to be used for microbial immobilized natural wooden fiber's carrier and preparation method thereof Expired - Fee Related CN106926334B (en)

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