CN106924138A - A kind of preparation method of Chinese herbal medicine active matter - Google Patents
A kind of preparation method of Chinese herbal medicine active matter Download PDFInfo
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- CN106924138A CN106924138A CN201710178341.1A CN201710178341A CN106924138A CN 106924138 A CN106924138 A CN 106924138A CN 201710178341 A CN201710178341 A CN 201710178341A CN 106924138 A CN106924138 A CN 106924138A
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- herbal medicine
- chinese herbal
- preparation
- active matter
- cell
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
Abstract
The present invention relates to cosmetic technical field, and in particular to a kind of preparation method of Chinese herbal medicine active matter, the method is comprised the following steps：Step one, sampling, step 2, sterilizing, step 3, culture, in step 2, the preparation of competent cell：1）Choose cell, 2）Secondary Culture, 3）Suspend culture, 4）Cleaning, 5）Dispersion, so as to obtain Chinese herbal medicine active matter, the method can be prepared as the Chinese herbal medicine active matter of cosmetic actives, effectively increase activity efficacy of the Chinese herbal medicine in cosmetics, and can simplify the preparation procedure of cosmetics.
Description
Technical field
The present invention relates to cosmetic material technical field, and in particular to a kind of preparation method of Chinese herbal medicine active matter.
Background technology
The mankind come from and beautify the serious hope of itself, just there is constantly pursuit to cosmetics since ancient times.During using cosmetics, change
Cosmetic is applied directly on the skin of user, and most of composition of cosmetics is prepared from by chemical substance, thus
It can be seen that, the function of the existing protection of cosmetics and skin makeup, while can also noxious material be produced so that causing certain side effect, because
This, it is necessary to the composition of strict control cosmetics, to balance the skin makeup performance and Green performance of cosmetics.Moreover, with people
The raising of quality of life, consumer has not stayed in skin makeup effect aspect to the consumption idea of cosmetics, to the day of cosmetics
Right performance also has larger weight to pursue, therefore, prepare naturally and the good cosmetics of skin makeup effect have turned into cosmetic industry
Long-sought.The existing technique for preparing natural health cosmetics is mainly and replaces chemical raw material using green Organic Ingredients, and
The green Organic Ingredients of addition is that directly the self component of green Organic Ingredients is processed with the bulk composition of cosmetics, Jin Ersheng
Output green natural cosmetics popular to people.
However, the technique that green Organic Ingredients self component prepares cosmetics is had the following disadvantages it by above-mentioned direct addition
Place：First, green Organic Ingredients is essentially from full grown plant, but full grown active components of plants is few, no
Beneficial to the activity efficacy for improving cosmetics；Second, during using green Organic Ingredients for adding ingredient, also need first to green organic original
Material carries out multiple pre-treatment and just can serve as preparing cosmetics, and these pre-treatment steps extend the production line for preparing cosmetics
Journey, is unfavorable for simplifying the preparation procedure of cosmetics.
The content of the invention
There is above-mentioned technical problem for prior art, present invention aim at a kind of preparation of Chinese herbal medicine active matter of offer
Method, the method can be prepared as the Chinese herbal medicine active matter of cosmetic actives, effectively increased Chinese herbal medicine and made up
Activity efficacy in product, and the preparation procedure of cosmetics can be simplified.
To achieve the above object, the present invention provides following technical scheme：
A kind of preparation method of Chinese herbal medicine active matter is provided, is comprised the following steps：
Step one, sampling：The Chinese herbal medicine with vital activity is won for raw material；
Step 2, sterilizing：By Chinese herbal medicine sterilization described in step one, the Chinese herbal medicine after sterilizing is then cut
Histoorgan be explant；
Step 3, culture：Explant obtained by step 2 is placed in Fiber differentiation in N6 solid mediums, Chinese herbal medicine is obtained
Callus；
Step 4, the preparation of competent cell：
1）Choose cell：The preferable cell mass of body is chosen from the callus obtained by step 3；
2）Secondary Culture：The preferable cell mass Secondary Culture of form, so as to obtain the cell mass of loose particles shape；
3）Suspend culture：Shaking culture is carried out during the cell mass of loose particles shape is moved to the shaking flask equipped with nutrient solution, is swum
From cell；
4）Screening：Free plant cell is filtered, the cell mass of screening individual cells or peanut is used as competent cell；
Step 5, cleaning：Competent cell obtained by step 4 is placed on gauze, is then rinsed with clear water, so as to remove culture
Impurity in liquid, obtains clean competent cell；
Step 6, dispersion：Clean competent cell obtained by step 5 is scattered in glycerine, Chinese herbal medicine active matter is obtained.
Wherein, the Chinese herbal medicine is any one in sarranine flower pesticide, ginkgo, french rose.
Wherein, include first cleaning Chinese herbal medicine with alcohol in the step 2, the step of sterilization, then by medium-height grass
Medicine plant is soaked in the supernatant of saturation bleaching powder, then through sterile water wash, the Chinese herbal medicine of the sterilizing that is sterilized.
Wherein, in the step 2, the concentration of the alcohol is 40 ~ 80%.
Wherein, in the step 2, soak time of the Chinese herbal medicine in the supernatant of saturation bleaching powder be 5 ~
15min。
Wherein, in the step 2, the number of times of sterile water wash is 2 ~ 5 times.
Wherein, in the step 3, the indoles second that mass fraction is 0.05 ~ 0.3% is contained in the N6 solid mediums
Acid, the 6- benzyl purines that mass fraction is 0.01 ~ 0.5%, the gibberellic acid that mass fraction is 0.01 ~ 0.2%, mass fraction are 0.1
~ 1.0% fructose.
Wherein, in the step 3, the explant of the gained of UV uviol lamps induction step two is used.
Wherein, in the step 3, the time of Fiber differentiation is 2 ~ 5 weeks.
Wherein, in the step 4, the passage number of the Secondary Culture was 2 ~ 4 generations.
Beneficial effects of the present invention：
（1）A kind of preparation method of Chinese herbal medicine active matter of the invention, the method is cultivated by from the tissue of Chinese herbal medicine
Competent cell is obtained, then competent cell is extracted and is placed in distributed and saved in glycerine, so as to obtain Chinese herbal medicine active matter.With it is existing
There is technology to compare, the preparation method cultivates the Chinese herbal medicine competent cell of ecosystem as active matter, it is ensured that Chinese herbal medicine is being changed
Activity efficacy in cosmetic, also, the method disperses Chinese herbal medicine competent cell using glycerine, not only can for a long time keep Chinese herbal medicine to live
Property thing quality, and cause Chinese herbal medicine active matter finished product, i.e. production cosmetics when, directly the active matter in glycerine is added
To enter can be used as in cosmetic material and produce cosmetics, without the tedious steps by extracting Chinese herbal medicine, and then simplifying
The preparation procedure of cosmetic, additionally, the Chinese herbal medicine active matter of finished product is available for dosage on demand, and then improves artificial regulatory cosmetics
Controllability.
（2）The preparation method of a kind of Chinese herbal medicine active matter that the present invention is provided, with operating method is simple, production cost
It is low and can be applied to large-scale production the characteristics of.
Specific embodiment
Embodiment 1.
A kind of preparation method of Chinese herbal medicine active matter is provided, is comprised the following steps：
Step one, sampling：The kind herbal plants with vital activity are won for raw material, it is preferable that the Chinese herbal medicine is sarranine
Flower；
Step 2, sterilizing：By sarranine flowering plant sterilization described in step one, specifically, the step of sterilization include：First use
40% alcohol cleans safron plant, and sarranine flowering plant then is soaked in into 5min in the supernatant of saturation bleaching powder, then passes through
Sterile water wash 2 times, the sarranine flowering plant of the sterilizing that is sterilized, then cutting the histoorgan of sarranine flowering plant after sterilizing is
Explant；
Step 3, culture：Explant obtained by step 2 is placed in UV uviol lamps Fiber differentiation 2 is used in N6 solid mediums
In week, obtain the callus of safron；
Wherein, to contain heteroauxin that mass fraction is 0.05%, the 6- benzyls that mass fraction is 0.01% fast for N6 solid mediums
Purine, mass fraction are 0.01% gibberellic acid, the adding ingredient of the fructose that mass fraction is 0.1%, and the adding ingredient is that production is adjusted
Section agent, can induce the growth of histoorgan；
Step 4, the preparation of competent cell：
1）Choose cell：The preferable cell mass of body, the preferable cell mass of form are chosen from the callus obtained by step 3
With preferable activity, beneficial to subsequent production；
2）Secondary Culture：, cell is in undifferentiated vegetative state in the form preferable generation of cell mass Secondary Culture 2, so that
Obtain the cell mass of loose particles shape；
3）Suspend culture：Shaking culture is carried out during the cell mass of loose particles shape is moved to the shaking flask equipped with nutrient solution, is swum
From cell；
4）Screening：Free plant cell is filtered, the cell mass of individual cells or peanut is filtered out as competent cell；
Step 5, cleaning：Competent cell obtained by step 4 is placed on gauze, is then rinsed with clear water, to remove nutrient solution
In impurity, obtain clean competent cell；
Step 6, dispersion：Clean competent cell obtained by step 5 is scattered in glycerine, safron Chinese herbal medicine activity is obtained
Thing.
Embodiment 2.
A kind of preparation method of Chinese herbal medicine active matter is provided, is comprised the following steps：
Step one, sampling：The Chinese herbal medicine with vital activity is won for raw material, it is preferable that the Chinese herbal medicine is ginkgo；
Step 2, sterilizing：By described in step one kinds of Gingko biloba plant sterilization, specifically, the step of sterilization include：First use
80% alcohol cleans ginkgo rose plant, Gingko biloba plant then is soaked in into 15min in the supernatant of saturation bleaching powder, then
Through sterile water wash 5 times, the Gingko biloba plant of the sterilizing that is sterilized, it is outer then to cut the histoorgan of the Gingko biloba plant after sterilizing
Implant；
Step 3, culture：Explant obtained by step 2 is placed in UV uviol lamps Fiber differentiation 3 is used in N6 solid mediums
In week, obtain the callus of ginkgo；
Wherein, to contain heteroauxin that mass fraction is 0.3%, the 6- benzyls that mass fraction is 0.5% fast for N6 solid mediums
Purine, mass fraction are 0.2% gibberellic acid, the adding ingredient of the fructose that mass fraction is 1.0%, and the adding ingredient is production regulation
Agent, can induce the growth of histoorgan；
Step 4, the preparation of competent cell：
1）Choose cell：The preferable cell mass of body is chosen from the callus obtained by step 3；
2）Secondary Culture：, cell is in undifferentiated vegetative state in the form preferable generation of cell mass Secondary Culture 4, so that
Obtain the cell mass of loose particles shape；
3）Suspend culture：Shaking culture is carried out during the cell mass of loose particles shape is moved to the shaking flask equipped with nutrient solution, is swum
From cell；
4）Screening：Free plant cell is filtered, the cell mass of individual cells or peanut is filtered out as competent cell；
Step 5, cleaning：Competent cell obtained by step 4 is placed on gauze, is then rinsed with clear water, to remove nutrient solution
In impurity, obtain clean competent cell；
Step 6, dispersion：Clean competent cell obtained by step 5 is scattered in glycerine, ginkgo Chinese herbal medicine active matter is obtained.
Embodiment 3.
A kind of preparation method of Chinese herbal medicine active matter is provided, is comprised the following steps：
Step one, sampling：The Chinese herbal medicine with vital activity is won for raw material, it is preferable that the Chinese herbal medicine is France
Rose plant；
Step 2, sterilizing：By french rose plant sterilization described in step one, specifically, the step of sterilization include：First
Safron, ginkgo, french rose plant are cleaned with 60% alcohol, then by safron, ginkgo, the leaching of french rose plant
The 10min in the supernatant of saturation bleaching powder is steeped, then through sterile water wash 3 times, the french rose plant of the sterilizing that is sterilized,
Then the histoorgan for cutting the french rose plant after sterilizing is explant；
Step 3, culture：Explant obtained by step 2 is placed in UV uviol lamps Fiber differentiation 4 is used in N6 solid mediums
In week, obtain the callus of french rose plant；
Wherein, to contain heteroauxin that mass fraction is 0.15%, the 6- benzyls that mass fraction is 0.25% fast for N6 solid mediums
Purine, mass fraction are 0.1% gibberellic acid, the adding ingredient of the fructose that mass fraction is 0.08%, and the adding ingredient is that production is adjusted
Section agent, can induce the growth of histoorgan；
Step 4, the preparation of competent cell：
1）Choose cell：The preferable cell mass of body is chosen from the callus obtained by step 3；
2）Secondary Culture：, cell is in undifferentiated vegetative state in the form preferable generation of cell mass Secondary Culture 3, so that
Obtain the cell mass of loose particles shape；
3）Suspend culture：Shaking culture is carried out during the cell mass of loose particles shape is moved to the shaking flask equipped with nutrient solution, is swum
From cell；
4）Screening：Free plant cell is filtered, the cell mass of individual cells or peanut is filtered out as competent cell；
Step 5, cleaning：Competent cell obtained by step 4 is placed on gauze, is then rinsed with clear water, to remove nutrient solution
In impurity, obtain clean competent cell；
Step 6, dispersion：Clean competent cell obtained by step 5 is scattered in glycerine, french rose Chinese herbal medicine activity is obtained
Thing.
Finally it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than the present invention is protected
The limitation of scope is protected, although being explained to the present invention with reference to preferred embodiment, one of ordinary skill in the art should
Work as understanding, technical scheme can be modified or equivalent, without deviating from the reality of technical solution of the present invention
Matter and scope.
Claims (9)
1. a kind of preparation method of Chinese herbal medicine active matter, it is characterized in that：Comprise the following steps：
Step one, sampling：The Chinese herbal medicine with vital activity is won for raw material；
Step 2, sterilizing：By Chinese herbal medicine sterilization described in step one, the Chinese herbal medicine after sterilizing is then cut
Histoorgan be explant；
Step 3, culture：Explant obtained by step 2 is placed in Fiber differentiation in N6 solid mediums, Chinese herbal medicine is obtained
Callus；
Step 4, the preparation of competent cell：
1）Choose cell：The preferable cell mass of body is chosen from the callus obtained by step 3；
2）Secondary Culture：The preferable cell mass Secondary Culture of form, so as to obtain the cell mass of loose particles shape；
3）Suspend culture：Shaking culture is carried out during the cell mass of loose particles shape is moved to the shaking flask equipped with nutrient solution, is swum
From cell；
4）Screening：Free plant cell is filtered, the cell mass of screening individual cells or peanut is used as competent cell；
Step 5, cleaning：Competent cell obtained by step 4 is placed on gauze, is then rinsed with clear water, so as to remove culture
Impurity in liquid, obtains clean competent cell；
Step 6, dispersion：Clean competent cell obtained by step 5 is scattered in glycerine, Chinese herbal medicine active matter is obtained.
2. the preparation method of a kind of Chinese herbal medicine active matter according to claim 1, it is characterized in that：In the step 2, disappear
The step of poison sterilizing, includes first cleaning Chinese herbal medicine with alcohol, and then Chinese herbal medicine is soaked in the supernatant of saturation bleaching powder
In liquid, then through sterile water wash, the Chinese herbal medicine of the sterilizing that is sterilized.
3. the preparation method of a kind of Chinese herbal medicine active matter according to claim 2, it is characterized in that：In the step 2, institute
The concentration for stating alcohol is 40 ~ 80%.
4. the preparation method of a kind of Chinese herbal medicine active matter according to claim 2, it is characterized in that：In the step 2, institute
It is 5 ~ 15min to state soak time of the Chinese herbal medicine in the supernatant of saturation bleaching powder.
5. according to a kind of preparation method of Chinese herbal medicine active matter according to claim 3, it is characterized in that：The step 2
In, the number of times of sterile water wash is 2 ~ 5 times.
6. the preparation method of a kind of Chinese herbal medicine active matter according to claim 1, it is characterized in that：In the step 3, institute
State in N6 solid mediums containing mass fraction for 0.05 ~ 0.3% heteroauxin, mass fraction for 0.01 ~ 0.5% 6- benzyls
Gibberellic acid, the fructose that mass fraction is 0.1 ~ 1.0% of base purine, mass fraction for 0.01 ~ 0.2%.
7. the preparation method of a kind of Chinese herbal medicine active matter according to claim 1, it is characterized in that：In the step 3, make
With the explant of the gained of UV uviol lamps induction step two.
8. the preparation method of a kind of Chinese herbal medicine active matter according to claim 1, it is characterized in that：In the step 3, lure
The time for leading culture is 2 ~ 5 weeks.
9. the preparation method of a kind of Chinese herbal medicine active matter according to claim 1, it is characterized in that：In the step 4, institute
The passage number for stating Secondary Culture was 2 ~ 4 generations.
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| CN201710178341.1A CN106924138A (en) | 2017-03-23 | 2017-03-23 | A kind of preparation method of Chinese herbal medicine active matter |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1416806A (en) * | 2001-11-09 | 2003-05-14 | 埃文产品公司 | Local composition with undifferentiated plant seed cell and ifs usage |
| CN103109747A (en) * | 2013-03-10 | 2013-05-22 | 通化师范学院 | Rapid pseudolarix propagation method based on stem node propagation |
| CN104783324A (en) * | 2015-03-17 | 2015-07-22 | 湖北中烟工业有限责任公司 | Method for preparing tobacco cell extract |
-
2017
- 2017-03-23 CN CN201710178341.1A patent/CN106924138A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1416806A (en) * | 2001-11-09 | 2003-05-14 | 埃文产品公司 | Local composition with undifferentiated plant seed cell and ifs usage |
| CN103109747A (en) * | 2013-03-10 | 2013-05-22 | 通化师范学院 | Rapid pseudolarix propagation method based on stem node propagation |
| CN104783324A (en) * | 2015-03-17 | 2015-07-22 | 湖北中烟工业有限责任公司 | Method for preparing tobacco cell extract |
Non-Patent Citations (1)
| Title |
|---|
| 刘新: "《植物生理学实验指导》", 31 January 2015, 中国农业出版社 * |
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Application publication date: 20170707 |
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