CN106912501A - The compounded pesticides of Lecanicillium lecanii and rotenone - Google Patents
The compounded pesticides of Lecanicillium lecanii and rotenone Download PDFInfo
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- CN106912501A CN106912501A CN201710299239.7A CN201710299239A CN106912501A CN 106912501 A CN106912501 A CN 106912501A CN 201710299239 A CN201710299239 A CN 201710299239A CN 106912501 A CN106912501 A CN 106912501A
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- Prior art keywords
- rotenone
- lecanicillium lecanii
- compounded pesticides
- host
- lecanicillium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
Abstract
The invention discloses the compounded pesticides and its application of a kind of Lecanicillium lecanii and rotenone.The compounded pesticides are made up of host and assistant agent, and by mass percentage, host is 10~40%, and assistant agent is 60~90%；The host is the mixture of Lecanicillium lecanii and rotenone, Lecanicillium lecanii: the mass ratio of rotenone is 10: 1.Compounded pesticides of the invention can be processed into wettable powder, and the compounded pesticides have efficient prevention effect to insect aleyrodid and aphid.
Description
Technical field
The invention belongs to technical field of pesticide, it is related to the compounded pesticides of a kind of environment amenable Lecanicillium lecanii and rotenone.
Background technology
Lecanicillium lecanii（Lecanicillium lecanii）It is a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals,
With the brand-new mechanism of action different from chemical insecticide, environment amenable feature, can as prevent and treat some to chemistry
The replacement protective agents of the insect that insecticide develops immunity to drugs, developing Lecanicillium lecanii has important economic implications and ecological meaning
Justice.Lecanicillium lecanii geographical distribution extensively, can be used for various important agricultural pests such as preventing and treating scale insect, aleyrodid, aphid, wherein writing
Name is to prevent and treat the aleyrodid and aphid in warmhouse booth, with good application effect.Scientific investigations showed that, different geographic populations
Or the Lecanicillium lecanii of different strains has larger difference to the virulence of insect, even if the Lecanicillium lecanii of same strain does harm to variety classes
The virulence of worm is also differed, therefore often to be sieved for different types of insect during biological control is carried out using fungi
Select supper toxic strain.Lecanicillium lecanii there is also and knock down that the insect time is long, desinsection speed slow, by environment as other entomogenous fungis
The shortcomings of influenceing larger, especially when insect density is larger, is difficult to force down insect population in time, retrieves a loss.
Rotenone（Rotenone）, it is a kind of insecticide, molecular formula C23H22O6, it is widely present in Tropical Asian and subtropical zone
Area produces pulse family Derris plant roots skin zone, also contains in some Chinese herbal medicines such as wayaka yambean seed, thickfruit millettia seed or fruit, leatherleaf millettia root,
It is a kind of very strong material of specificity in toxicology, to insect, especially cabbage caterpillar, diamondback moth and aphid have strongly
Tag and the effect of two kinds of stomach toxicity.Scientific investigations showed that, the mechanism of action of rotenone is mainly the respiration of influence insect, makes
The electron transport chain of pest cell is suppressed, so as to the ATP levels reduced in organism finally make insect cannot get energy supply
Should, then drumble, paralysis and it is slow dead.Rotenone crop pests preventing and treating in have it is environmentally friendly, it is micro- poison, no
The advantages of easily making insect develop immunity to drugs, is to produce the pesticide species that non-polluted farm product is preferentially selected.But, pure plant source agriculture
Medicine is similar to Chinese medicine, comes compared with chemical pesticide, and toxicity is low, the lasting period is short, and because raw material all is from naturally, seriously limiting plant
The yield of source pesticide, restricts using and promoting for botanical pesticide.
Lecanicillium lecanii can in practice substantially reduce the consumption of rotenone with rotenone compounding use in agricultural production, delay insect
The process of resistance is produced to rotenone, the rotenone of sub-doses mixes with Lecanicillium lecanii can also effectively improve the quick-acting of fungus insecticide
Property, while protecting ecological environment.Therefore, the optimal compound proportion of screening Lecanicillium lecanii and rotenone, with important application
Meaning.Have no the relevant report of the insecticide of Lecanicillium lecanii and rotenone compounding both at home and abroad at present.
The content of the invention
It is an object of the invention to overcome the deficiencies in the prior art, there is provided a kind of environment amenable Lecanicillium lecanii and rotenone
Compounded pesticides.
Another object of the present invention is to provide the application of the compounded pesticides.
Above-mentioned purpose of the present invention is achieved through the following technical solutions：
The invention provides a kind of Lecanicillium lecanii and the compounded pesticides of rotenone, it is made up of host and assistant agent, by mass percentage
Meter, host is 10~40%, and assistant agent is 60~90%；The host is the mixture of Lecanicillium lecanii and rotenone, Lecanicillium lecanii: rotenone
Mass ratio be 50: 1~10.
Described lecanium bacteria strain GLLE0906, is located away from the aleyrodid polypide that Langfang in Hebei Province area is infected, and bacterial strain is protected
Tibetan number is CGMCC No.8453, and on November 11st, 2013 is in China Committee for Culture Collection of Microorganisms's common micro-organisms
Heart preservation.Lecanium bacteria strain GLLE0906 be screened from numerous similar bacterial strains, dedicated for the preventing and treating insect such as aleyrodid
Lecanicillium lecanii sociales bacterial strain, with virulence it is strong, desinsection speed is fast, it is environmentally friendly the features such as, and obtain the hair of country's mandate
Bright patent（ZL201310560723.2）.
In order to the present invention is better achieved, the Lecanicillium lecanii is preferably 50: 2~6 with the mass ratio of rotenone.
As one kind preferably, the Lecanicillium lecanii is Lecanicillium lecanii conidial powder.
The assistant agent is stabilizer, auxiliary agent and filler, and by mass percentage, stabilizer accounts for compounded pesticides 1 ~ 10%, helps
Agent accounts for compounded pesticides 1 ~ 20%, and filler accounts for compounded pesticides 30 ~ 88%.
The stabilizer is glucose（Powder）With one or two mixture of starch；The auxiliary agent is washing powder, ten
Dialkyl sulfonates, sodium lignin sulfonate, bay alcohol radical sodium sulphate, alkylsulfonate, wetting agent BENOL790A, dispersant
One or more mixtures in BD55；Described filler is white carbon, talcum powder, potter's clay, diatomite, kaolin or viscous
Soil.
Compounded pesticides of the present invention can be processed into wettable powder.
Present invention simultaneously provides application of the compounded pesticides in pest control aleyrodid and aphid.
The present invention compared with prior art, has the following advantages that and beneficial effect：
The bacterial strain that the present invention is used is to separate to obtain on the aleyrodid polypide infected from Langfang in Hebei Province area, is China domestic bacterium
Strain, not introduces from foreign countries, can be well adapted for local natural environment.The present invention has adapted to the demand of organic foodstuff production,
Environmentally safe, noresidue, is conducive to delaying pest resistance to insecticide to produce.There is compounded pesticides of the present invention significant synergy to make
With the prevention effect to insect is better than the single kind of prevention effect of medicament, has good prevention effect to insect aleyrodid and aphid.
Specific embodiment
Inventor has found the mixture of Lecanicillium lecanii and rotenone to insect aleyrodid and aphid by substantial amounts of Seiving Tests
With synergistic function.In order to be better understood from the essence of invention, detailed are made to technology contents of the invention with reference to example
Thin explanation, but present disclosure do not limit in this way, can not therefore and be interpreted as the limitation to the scope of the claims of the present invention.
Percentage is mass percent described in following examples.Described lecanium bacteria strain is GLLE0906, bacterial strain
Preserving number is CGMCC No.8453, and on November 11st, 2013 is in China Committee for Culture Collection of Microorganisms's common micro-organisms
Center preservation.
Embodiment 1：Lecanicillium lecanii and rotenone mixed ratio are screened
Subjects are Bemisia tabaci（Bemisia tabaci）Two age nymphs, test medicine is Lecanicillium lecanii, rotenone and Lecanicillium lecanii
With the mixture preparation of rotenone.Lecanicillium lecanii be 10,000,000,000 spores/gram pure cryptogam, rotenone for content 95% active compound.
Test method is with reference to People's Republic of China's agricultural industry criteria《Farm-chemical indoor determination test rule》Desinsection
Agent NY/T 1154.9-2008, experiment uses spraying treatment method.By the Lecanicillium lecanii spore suspension of series concentration, rotenone and wax
The mixture preparation miniaturised nebuliser of a red-spotted lizard bacterium and rotenone is sprayed on the cucumber leaves with Bemisia tabaci two ages nymph, and control is nothing
Bacterium water, dries naturally.3 leaves are often processed as, per 100 nymphs of leaf, 3 repetitions.In placing a plant into transparent freshness protection package, then
It is put into 24 ± 1 DEG C of illumination box（L∶D=16∶8）, the 7th day borer population of record death.Entered with DPS data processing softwares
Row statistical analysis, calculates the LC of each medicament50, the activity of reagent agent is evaluated with this.
The co-toxicity coefficient of mixture（CTC values）Calculated by following equation：
ATI = S / M × 100
In formula：ATI is that mixture surveys toxicity index；S is the LC of standard pesticide agent50, unit is mg/L；M is the LC of mixture50, it is single
Position is mg/L.
TTI = TIA × PA + TIB × PB
In formula：TTI is mixture theoretical toxicity index；TIA is A medicament toxicity index；PA contains for percentage of the A medicaments in mixture
Amount, unit is percentage；TIB is B medicament toxicity index；PB is percentage composition of the B medicaments in mixture, and unit is percentage.
CTC = ATI / TTI × 100
In formula：CTC is co-toxicity coefficient；ATI is that mixture surveys toxicity index；TTI is mixture theoretical toxicity index.
CTC >=120 show as synergistic effect；CTC≤80 show as antagonism；80 < CTC <120 tables
It is now summation action.
Table 1：Each single dose and its mixture are to the Bemisia tabaci two ages nymph toxicity test result of 7 days
| Medicament title | ATI | TTI | Co-toxicity coefficient CTC | |
| Lecanicillium lecanii (A) | 9.50 | 100.00 | - | - |
| Rotenone (B) | 3.88 | 244.85 | - | - |
| A∶B = 20∶1 | 6.76 | 140.53 | 106.90 | 131.46 |
| A∶B = 10∶1 | 3.84 | 247.40 | 113.17 | 218.61 |
| A∶B = 1∶1 | 4.03 | 235.73 | 172.43 | 136.72 |
| A∶B = 1∶10 | 3.14 | 302.55 | 231.68 | 130.59 |
| A∶B = 1∶20 | 3.21 | 295.95 | 237.95 | 124.37 |
As shown in table 1, Toxicity Determination result shows result, and Lecanicillium lecanii and rotenone were to Bemisia tabaci two ages nymph 7 days
LC50Respectively 9.50mg/L and 3.88mg/L.Lecanicillium lecanii: when rotenone mass ratio is 20: 1 to 1: 20（That is the mitogenetic spore of Lecanicillium lecanii
When sub- number is 2000~10,000,000,000: 1~20 with rotenone grams ratio), co-toxicity coefficient（CTC）120 are all higher than, each proportioning is illustrated
Synergistic effect is respectively provided with, especially Lecanicillium lecanii: when rotenone mass ratio is 10: 1（That is Lecanicillium lecanii conidium number and rotenone
When grams ratio is 100,000,000,000: 1）, co-toxicity coefficient（CTC）More than 200, synergistic effect is notable.
Embodiment 2：10% Lecanicillium lecanii rotenone wettable powder and its preventive effect to aleyrodid on greenhouse cucumber
The pure cryptogam of Lecanicillium lecanii（10000000000 spores/gram）9.09%, rotenone（95% active compound level）0.91%, starch 3%, dispersant alkyl
Sulfonate 3%, dodecyl sodium sulfate 3%, kaolin are mended to 100%, and 10% Lecanicillium lecanii rotenone wettable powder is obtained.Mixture
Preparation adds water 3000 times and dilutes, Bemisia tabaci on spraying preventing and treating greenhouse cucumber（Bemisia tabaci）, when greenhouse, indoor temperature is
24 DEG C, during relative humidity 85%, the accumulative prevention effect of 7 days can reach 92.8%.
Embodiment 3：10% Lecanicillium lecanii rotenone wettable powder and its preventive effect to aphid on greenhouse cucumber
The pure cryptogam of Lecanicillium lecanii（10000000000 spores/gram）9.09%, rotenone（95% active compound level）0.91%, starch 3%, dispersant
WLND100 3%, dodecyl sodium sulfate 3%, white carbon are mended to 100%, and 10% Lecanicillium lecanii rotenone wettable powder is obtained.It is mixed
Preparaton adds water 3000 times and dilutes, aphid on spraying preventing and treating greenhouse cucumber（Aphis gossypii）, when greenhouse, indoor temperature is
24 DEG C, during relative humidity 85%, the accumulative prevention effect of 7 days can reach 98.5%.
Claims (2)
1. compounded pesticides of a kind of Lecanicillium lecanii and rotenone, it is characterised in that the compounded pesticides are wettable powder, by
Host and assistant agent are constituted, and by mass percentage, host is 10~40%, and assistant agent is 60~90%；
The host is the mixture of Lecanicillium lecanii and rotenone, Lecanicillium lecanii: the mass ratio of rotenone is 10: 1；
Described Lecanicillium lecanii is bacterial strain GLLE0906, and bacterial strain preserving number is CGMCC No.8453, and on November 11st, 2013 is in China
Microbiological Culture Collection administration committee common micro-organisms center preservation.
2. application of the compounded pesticides described in claim 1 in pest control aleyrodid and aphid.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710299239.7A CN106912501B (en) | 2017-05-02 | 2017-05-02 | The compounded pesticides of Lecanicillium lecanii and rotenone |
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| CN201710299239.7A CN106912501B (en) | 2017-05-02 | 2017-05-02 | The compounded pesticides of Lecanicillium lecanii and rotenone |
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| CN106912501B CN106912501B (en) | 2018-02-13 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112514919A (en) * | 2020-11-26 | 2021-03-19 | 赞皇县龙堂苑农业科技有限责任公司 | Compound agent of verticillium lecanii and propargite |
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| CN101658186A (en) * | 2009-09-16 | 2010-03-03 | 甘肃省科学院生物研究所 | Verticillium lecanii quick-release dispersible tablet and preparation method thereof |
| CN102863284A (en) * | 2012-09-24 | 2013-01-09 | 华南农业大学 | Organic fertilizer and preparation method thereof and method for controlling soil borne disease |
| CN104195047A (en) * | 2013-11-13 | 2014-12-10 | 谢明 | Lecanicillium lecanii and application of Lecanicillium lecanii to control of greenhouse pests |
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| CN104255808A (en) * | 2014-08-25 | 2015-01-07 | 谢明 | Lecanicillium lecanii oil solution, preparing method thereof and applications of the oil solution in agricultural pest control |
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2017
- 2017-05-02 CN CN201710299239.7A patent/CN106912501B/en not_active Expired - Fee Related
Patent Citations (6)
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|---|---|---|---|---|
| DE19707178A1 (en) * | 1997-02-22 | 1998-08-27 | Hauptmann Georg Gerhart Dipl B | Insecticidal or acaricidal plant protectant composition |
| CN101658186A (en) * | 2009-09-16 | 2010-03-03 | 甘肃省科学院生物研究所 | Verticillium lecanii quick-release dispersible tablet and preparation method thereof |
| CN102863284A (en) * | 2012-09-24 | 2013-01-09 | 华南农业大学 | Organic fertilizer and preparation method thereof and method for controlling soil borne disease |
| CN104195047A (en) * | 2013-11-13 | 2014-12-10 | 谢明 | Lecanicillium lecanii and application of Lecanicillium lecanii to control of greenhouse pests |
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Non-Patent Citations (1)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112514919A (en) * | 2020-11-26 | 2021-03-19 | 赞皇县龙堂苑农业科技有限责任公司 | Compound agent of verticillium lecanii and propargite |
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Granted publication date: 20180213 Termination date: 20190502 |