CN106906130A - A kind of flat PCR reaction tubes of printing opacity high - Google Patents
A kind of flat PCR reaction tubes of printing opacity high Download PDFInfo
- Publication number
- CN106906130A CN106906130A CN201710258281.4A CN201710258281A CN106906130A CN 106906130 A CN106906130 A CN 106906130A CN 201710258281 A CN201710258281 A CN 201710258281A CN 106906130 A CN106906130 A CN 106906130A
- Authority
- CN
- China
- Prior art keywords
- hypomere
- epimere
- printing opacity
- pcr reaction
- reaction tubes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
- B01L3/50825—Closing or opening means, corks, bungs
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/046—Function or devices integrated in the closure
- B01L2300/047—Additional chamber, reservoir
Abstract
The invention discloses a kind of flat PCR reaction tubes of printing opacity high, including body and the upper lid being articulated with the side of body top, body is followed successively by body epimere, changeover portion and body hypomere from top to bottom, and body epimere and body hypomere are connected by changeover portion；Body epimere is cylindrical structure, and body hypomere is rectangular structure；The reaction tube is used in isothermal PCR detection process as test sample container, using the structure setting of circular upper part and square lower part, both the cheap property that round mouth is used had been retained, again for the transmitted light that test is used provides the square duct that can vertically pass in and out, scattering and weakening of the arcuate structure to light are avoided, influence of the reaction tube body to testing is greatly reduced.
Description
Technical field
The invention belongs to test consumptive material technical field, and in particular to a kind of flat PCR reaction tubes of printing opacity high.
Background technology
The serial equipments such as isothermal PCR instrument, its principle is that target unit content is detected using the method for fluorogenic quantitative detection.
In practical application, PCR reaction tubes are used as test solution carrier.As shown in figure 5, the reaction used to be circulated in current industry
Tubular construction, it is cone, and this kind of structure has obvious scattering to light, as shown in fig. 6, being the light path of conical structure
Figure, divergence of beam projection after the reacted pipe of light, the amount light for collecting test equipment has declined, and test is have impact on indirectly
Effect, is easily caused larger measurement error.
The content of the invention
In order to overcome the shortcomings of that reaction tube body influences on light, for test equipment provides good test condition, reduce
The error of measurement, the present invention provides a kind of flat PCR reaction tubes of printing opacity high, reaction tube conduct in isothermal PCR detection process
Test sample container is used, and using the structure setting of circular upper part and square lower part, has both retained the cheap property that round mouth is used, and for test is used
Transmitted light provide the square duct that can vertically pass in and out, it is to avoid scattering and weakening of the arcuate structure to light, substantially reduce
Influence of the reaction tube body to testing.
The technical solution adopted for the present invention to solve the technical problems is：
A kind of flat PCR reaction tubes of printing opacity high, including body and the upper lid being articulated with the side of body top, body are used to contain
Testing liquid, upper lid is added a cover in body upper end, it is ensured that whole reaction tube is preferably sealed, and body is using refractive index is small, transparency
Environment-friendly materials high are made, and can be preferably minimized the loss of transmitted ray, preferably improve Detection results.
Described body using circular upper part and square lower part topology layout, body be followed successively by from top to bottom body epimere, changeover portion and
Body hypomere, body epimere and body hypomere are connected by changeover portion.
Described body epimere is cylindrical structure, and round mouth is provided with a circle using more convenient on body epimere inwall
Lower convexity.
Described changeover portion is made up of smooth flat.
Described body hypomere is rectangular structure, and body hypomere is the transmission region of test equipment, before the hypomere of body
Transmission plane afterwards is parallel, can be preferably minimized the light losing of transmission.
Described upper lid includes a hollow circuit cylinder, and the external diameter of hollow circuit cylinder is identical with the internal diameter of body epimere, hollow circle
Column outer wall is provided with the upper projection that a circle matches with lower convexity；Hollow circuit cylinder is inserted into intersegmental part on body, upper raised with
Projection is cooperated and snaps connection, and the sealing to body is covered in realization, and sealing effectiveness is preferable, it is ensured that in process of the test middle tube body
Liquid will not be excessive, improve detection precision, it is to avoid measurement apparatus error.
Preferably, the bottom side length with diameter greater than body hypomere of body epimere, facilitates testing crew to add testing liquid
To tubular body, body hypomere is the transmission region of test equipment, and can meet detection using less prepare liquid requires, meets
The detection demand of few liquid measure to be measured in part test.
Preferably, the length of described body hypomere between 3.8-4.2mm, body hypomere width between 2.8-3.2mm,
Between 6.8-7.2mm, applicant show that the size that body hypomere is set can expire to the height of body hypomere by substantial amounts of experiment
The test requirements document of foot more than 90%.
It is an advantage of the invention that：Structure of the present invention is novel, and body uses the structure design of circular upper part and square lower part, and both having retained round mouth makes
Cheap property, and for the transmitted light that uses of test provides the square duct that can vertically pass in and out, it is to avoid arcuate structure is to light
The scattering and weakening of line, greatly reduce influence of the reaction tube body to testing.
Brief description of the drawings
Fig. 1 is stereogram of the invention；
Fig. 2 is present invention sectional view vertically；
Fig. 3 is index paths of the Fig. 1 using process middle tube body hypomere；
Fig. 4 is the attachment structure schematic diagram of the reaction tube in specific embodiment；
Fig. 5 is the stereogram of the interior reaction tube for using that circulates of current industry in background technology；
Fig. 6 is the index path of conical structure in Fig. 5；
Fig. 7 is the exploded view of experimental model used during confirmatory experiment.
Specific embodiment
Below in conjunction with accompanying drawing, the present invention is further illustrated：
As Figure 1-3, a kind of flat PCR reaction tubes of printing opacity high, including body 1 and be articulated with it is upper on the top side of body 1
Lid 2, body 1 contains testing liquid, and body 1 can be dropped to the loss of transmitted ray using the material that refractive index is small, transparency is high
It is minimum, preferably improve Detection results.
The bottom of the design middle tube body 1 is divided into the transmission region of test equipment, in order to the light losing of transmission is dropped to most
It is low, Detection results are preferably improved, described body 1 is followed successively by body epimere 10, changeover portion 11 and body hypomere from top to bottom
12, body epimere 10 and body hypomere 12 are connected by changeover portion 11；Described body epimere 10 is cylindrical structure, on body
A circle lower convexity 101 is provided with 10 inwalls of section；Described changeover portion 11 is made up of smooth flat；Described body hypomere 12 is
Rectangular structure.
In order to the liquid ensured in process of the test middle tube body 1 will not be excessive, it is ensured that the precision and effect of detection, it is to avoid survey
Amount directional error, described upper lid 2 includes a hollow circuit cylinder 20, the external diameter of hollow circuit cylinder 20 and the internal diameter phase of body epimere 10
Together, the outer wall of hollow circuit cylinder 20 is provided with upper raised 201 that a circle matches with lower convexity 101；Hollow circuit cylinder 20 is inserted into body
Inside epimere 10, upper raised 201 and lower convexity 101 cooperate snap connection, sealing of the lid 2 to body 1 in realization.
Testing crew adds inside body 1 testing liquid for convenience, while meeting the few liquid measure to be measured inspection in part
Survey demand, the bottom side length with diameter greater than body hypomere 12 of body epimere 10.
Applicant obtains the length when described body hypomere between 3.8-4.2mm by constantly exploration, body hypomere
Width between 2.8-3.2mm, body hypomere height between 6.8-7.2mm, disclosure satisfy that more than 90% detection demand.
As shown in figure 4, in order to be applied to existing testing equipment, the reaction tube connects formula layout and sets using eight.
Contrast experiment：
1st, experimental principle
In practical application, test equipment uses the principle of fluorogenic quantitative detection, by adding fluorophor in reaction system, profit
The whole reaction process of real-time monitoring is accumulated with fluorescence signal, quantitative analysis is carried out to unknown template finally by standard curve.Should
It is contrast test to test, and shadow is produced to fluorescence signal with ordinary conical shaped reaction pipe by analyzing the flat PCR reaction tubes of printing opacity high
Loud power, to determine the two good and bad characteristic in actual use.
2nd, process of the test
Test temperature：Room temperature；
Method of testing：The device used in test process is as shown in fig. 7, in figure, 71 is supply lines, and 72 is LED light source, and 73 is branch
Support housing, 74 is signal collecting element, and 75 is signal processing unit, and 76 is reaction tube standing groove.After items are ready, respectively
The flat PCR reaction tubes of printing opacity high and ordinary conical shaped reaction pipe are positioned in reaction tube standing groove 76, then experimental model is put
In in light tight space（Avoid influence of the extraneous light to testing）Turning circuit, the light that LED light source is produced passes through reaction tube, it
Afterwards through reaction tube light is by the acquisition and recording experimental data of signal collecting element 74 and is delivered to signal processing unit 75, by believing
Number analyzing and processing data of processing unit 75, and derive test result.
3rd, experimental data
3.1st, test parameters
3.2nd, test data analyzer
A, two kinds of reaction tubes have a certain degree of weakening to light；
B, for any same medium, conical reaction tube is all higher than the flat PCR reaction tubes of printing opacity high to the weakening degree of light
To the weakening degree of pipeline.
4th, conclusion
The flat PCR reaction tubes of printing opacity high have more excellent light transmission performance than plain taper bottom reaction tube, are more suitable for being applied to reality
In the detection process of border.
Finally it should be noted that：Obviously, above-described embodiment is only intended to clearly illustrate example of the present invention, and simultaneously
The non-restriction to implementation method.For those of ordinary skill in the field, can also do on the basis of the above description
Go out the change or variation of other multi-forms.There is no need and unable to be exhaustive to all of implementation method.And thus drawn
Obvious change that Shen goes out or among changing still in protection scope of the present invention.
Claims (3)
1. a kind of flat PCR reaction tubes of printing opacity high, including body and the upper lid being articulated with the side of body top, body is using folding
The environment-friendly materials that rate is small, transparency is high are penetrated to be made, it is characterised in that：
Described body is followed successively by body epimere, changeover portion and body hypomere from top to bottom, and body epimere and body hypomere pass through
Changeover portion is connected；
Described body epimere is cylindrical structure, and a circle lower convexity is provided with body epimere inwall；
Described changeover portion is made up of smooth flat；
Described body hypomere is rectangular structure；
Described upper lid includes a hollow circuit cylinder, and the external diameter of hollow circuit cylinder is identical with the internal diameter of body epimere, outside hollow circuit cylinder
Wall is provided with the upper projection that a circle matches with lower convexity；Hollow circuit cylinder is inserted into intersegmental part on body, upper raised and lower convexity
Mutual cooperation is snapped connection, and the sealing to body is covered in realization.
2. a kind of flat PCR reaction tubes of printing opacity high as claimed in claim 1, it is characterised in that body epimere with diameter greater than
The bottom side length of body hypomere.
3. a kind of flat PCR reaction tubes of printing opacity high as claimed in claim 2, it is characterised in that the length of described body hypomere
Between 3.8-4.2mm, between 2.8-3.2mm, the height of body hypomere is between 6.8-7.2mm for the width of body hypomere.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710258281.4A CN106906130A (en) | 2017-04-19 | 2017-04-19 | A kind of flat PCR reaction tubes of printing opacity high |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710258281.4A CN106906130A (en) | 2017-04-19 | 2017-04-19 | A kind of flat PCR reaction tubes of printing opacity high |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106906130A true CN106906130A (en) | 2017-06-30 |
Family
ID=59211181
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710258281.4A Pending CN106906130A (en) | 2017-04-19 | 2017-04-19 | A kind of flat PCR reaction tubes of printing opacity high |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106906130A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109735437A (en) * | 2019-01-28 | 2019-05-10 | 长春长光辰英生物科学仪器有限公司 | It is collected and the vessel and method that handle after a kind of ejection sorting of cell for cell |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69519783T2 (en) * | 1994-04-29 | 2001-06-07 | Perkin Elmer Corp | METHOD AND DEVICE FOR REAL-TIME DETECTION OF PRODUCTS OF NUCLEIC ACID AMPLIFICATION |
| US20070128080A1 (en) * | 2005-12-01 | 2007-06-07 | Eppendorf Ag | Lidded vessel |
| US20130217112A1 (en) * | 2010-10-14 | 2013-08-22 | Genereach Biotechnology Corp. | Reaction tube for performing isothermal polymerase chain reaction therein |
| CN203981568U (en) * | 2014-06-24 | 2014-12-03 | 深圳市人民医院 | A kind of printing opacity test cup with a lid |
| CN105039149A (en) * | 2015-07-20 | 2015-11-11 | 宁波大学 | Closed experiment system device for quickly identifying nucleic acid amplification products and application of closed experiment system device |
| EP2965816A1 (en) * | 2014-07-09 | 2016-01-13 | Eppendorf Ag | Reaction vessel with lid |
| CN105413599A (en) * | 2014-09-12 | 2016-03-23 | 安纳利蒂克耶拿股份公司 | Reaction vessel, reaction vessel arrangement and method for analyzing a substance |
| CN206692647U (en) * | 2017-04-19 | 2017-12-01 | 北京倍肯创新诊断技术研究院有限责任公司 | A kind of flat PCR reaction tubes of high printing opacity |
-
2017
- 2017-04-19 CN CN201710258281.4A patent/CN106906130A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69519783T2 (en) * | 1994-04-29 | 2001-06-07 | Perkin Elmer Corp | METHOD AND DEVICE FOR REAL-TIME DETECTION OF PRODUCTS OF NUCLEIC ACID AMPLIFICATION |
| US20070128080A1 (en) * | 2005-12-01 | 2007-06-07 | Eppendorf Ag | Lidded vessel |
| US20130217112A1 (en) * | 2010-10-14 | 2013-08-22 | Genereach Biotechnology Corp. | Reaction tube for performing isothermal polymerase chain reaction therein |
| CN203981568U (en) * | 2014-06-24 | 2014-12-03 | 深圳市人民医院 | A kind of printing opacity test cup with a lid |
| EP2965816A1 (en) * | 2014-07-09 | 2016-01-13 | Eppendorf Ag | Reaction vessel with lid |
| CN105413599A (en) * | 2014-09-12 | 2016-03-23 | 安纳利蒂克耶拿股份公司 | Reaction vessel, reaction vessel arrangement and method for analyzing a substance |
| CN105039149A (en) * | 2015-07-20 | 2015-11-11 | 宁波大学 | Closed experiment system device for quickly identifying nucleic acid amplification products and application of closed experiment system device |
| CN206692647U (en) * | 2017-04-19 | 2017-12-01 | 北京倍肯创新诊断技术研究院有限责任公司 | A kind of flat PCR reaction tubes of high printing opacity |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109735437A (en) * | 2019-01-28 | 2019-05-10 | 长春长光辰英生物科学仪器有限公司 | It is collected and the vessel and method that handle after a kind of ejection sorting of cell for cell |
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