CN206692647U - A kind of flat PCR reaction tubes of high printing opacity - Google Patents
A kind of flat PCR reaction tubes of high printing opacity Download PDFInfo
- Publication number
- CN206692647U CN206692647U CN201720413168.4U CN201720413168U CN206692647U CN 206692647 U CN206692647 U CN 206692647U CN 201720413168 U CN201720413168 U CN 201720413168U CN 206692647 U CN206692647 U CN 206692647U
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- China
- Prior art keywords
- hypomere
- epimere
- printing opacity
- reaction tubes
- pcr reaction
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- 238000006243 chemical reaction Methods 0.000 title claims abstract description 35
- 238000007639 printing Methods 0.000 title claims abstract description 14
- 239000000463 material Substances 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 238000012360 testing method Methods 0.000 abstract description 31
- 238000001514 detection method Methods 0.000 abstract description 12
- 238000000034 method Methods 0.000 abstract description 8
- 230000003313 weakening effect Effects 0.000 abstract description 6
- 230000000717 retained effect Effects 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 8
- 230000005540 biological transmission Effects 0.000 description 7
- 238000012545 processing Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 3
- 238000013461 design Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 238000013212 standard curve analysis Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
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- Devices For Use In Laboratory Experiments (AREA)
Abstract
The utility model discloses a kind of high flat PCR reaction tubes of printing opacity, including body and the upper lid that is articulated with the side of body top, body are followed successively by body epimere, changeover portion and body hypomere from top to bottom, and body epimere is connected with body hypomere by changeover portion；Body epimere is cylindrical structure, and body hypomere is rectangular parallelepiped structure；The reaction tube uses in isothermal PCR detection process as test sample container, using the structure setting of circular upper part and square lower part, both the cheap property that round mouth uses had been retained, again the square duct that can vertically pass in and out is provided to test the transmitted light used, scattering and weakening of the arcuate structure to light are avoided, greatly reduces influence of the reaction tube body to test.
Description
Technical field
The utility model belongs to experiment consumptive material technical field, and in particular to a kind of high flat PCR reaction tubes of printing opacity.
Background technology
The serial equipments such as isothermal PCR instrument, its principle are to detect target unit content using the method for fluorogenic quantitative detection.
In practical application, PCR reaction tubes are used as test solution carrier.As shown in figure 5, it is the reaction that uses of being circulated in current industry
Tubular construction, it is cone, and such a structure has obvious scattering to light, as shown in fig. 6, the light path for conical structure
Scheme, divergence of beam projects after the reacted pipe of light, has declined the amount light that test equipment collects, have impact on test indirectly
Effect, it is easily caused larger measurement error.
Utility model content
Light is influenceed in order to overcome the shortcomings of to react tube body, good test condition is provided for test equipment, reduced
The error of measurement, the utility model provide a kind of high flat PCR reaction tubes of printing opacity, and the reaction tube is in isothermal PCR detection process
Used as test sample container, using the structure setting of circular upper part and square lower part, both retained the cheap property that round mouth uses, be again test
The transmitted light used provides the square duct that can vertically pass in and out, and avoids scattering and weakening of the arcuate structure to light, significantly
Reduce influence of the reaction tube body to test.
Technical scheme is used by the utility model solves its technical problem：
A kind of flat PCR reaction tubes of high printing opacity, including body and the upper lid that is articulated with the side of body top, body are used for
Testing liquid is contained, upper lid is capped in body upper end, ensures that whole reaction tube preferably seals, and body is small, saturating using refractive index
The high environment-friendly materials of lightness are made, and can be preferably minimized the loss of transmitted ray, preferably improve Detection results.
Described body use circular upper part and square lower part topology layout, body be followed successively by from top to bottom body epimere, changeover portion and
Body hypomere, body epimere and body hypomere are connected by changeover portion.
Described body epimere is cylindrical structure, and round mouth use is more convenient, and a circle is provided with body epimere inwall
Lower convexity.
Described changeover portion is made up of smooth flat.
Described body hypomere is rectangular parallelepiped structure, and body hypomere is the transmission region of test equipment, before the hypomere of body
Transmission plane afterwards is parallel, can be preferably minimized the light losing of transmission.
Described upper lid includes a hollow circuit cylinder, and the external diameter of hollow circuit cylinder is identical with the internal diameter of body epimere, hollow circle
Column outer wall is provided with the upper projection that a circle matches with lower convexity；Hollow circuit cylinder is inserted into intersegmental part on body, upper raised with
Projection cooperates and snapped connection, and sealing of the lid to body in realization, sealing effectiveness is preferable, ensures in experiment process middle tube body
Liquid will not be excessive, improve the precision of detection, avoid measurement apparatus error.
Preferably, the diameter of body epimere is more than the bottom side length of body hypomere, facilitates testing crew to add testing liquid
To tubular body, body hypomere is the transmission region of test equipment, can meet testing requirements using less prepare liquid, is met
The detection demand of few liquid measure to be measured in part test.
Preferably, described body hypomere is grown between 3.8-4.2mm, the width of body hypomere between 2.8-3.2mm,
For the height of body hypomere between 6.8-7.2mm, applicant show that the size that body hypomere is set can expire by substantial amounts of experiment
The test requirements document of foot more than 90%.
The utility model has the advantages that：The utility model structure is novel, and body uses the structure design of circular upper part and square lower part, both protects
The cheap property that round mouth uses is stayed, the square duct that can vertically pass in and out is provided to test the transmitted light used again, avoids arc
Scattering and weakening of the structure to light, greatly reduce influence of the reaction tube body to test.
Brief description of the drawings
Fig. 1 is stereogram of the present utility model；
Fig. 2 is the sectional view of the utility model vertically；
Fig. 3 is the index path that Fig. 1 uses process middle tube body hypomere；
Fig. 4 is the attachment structure schematic diagram of the reaction tube in specific embodiment；
Fig. 5 is the stereogram of the reaction tube that the interior circulation of industry at present uses in background technology；
Fig. 6 is the index path of conical structure in Fig. 5；
Fig. 7 is the exploded view of the experimental model used during confirmatory experiment.
Embodiment
The utility model is further described below in conjunction with accompanying drawing：
As Figure 1-3, the flat PCR reaction tubes of a kind of high printing opacity, including body 1 and be articulated with the top side of body 1
Upper lid 2, body 1 contains testing liquid, and body 1, can be by the loss of transmitted ray using refractive index is small, transparency is high material
It is preferably minimized, preferably improves Detection results.
The bottom of the design middle tube body 1 is divided into the transmission region of test equipment, in order to which the light losing of transmission is dropped to most
It is low, Detection results are preferably improved, described body 1 is followed successively by body epimere 10, changeover portion 11 and body hypomere from top to bottom
12, body epimere 10 and body hypomere 12 are connected by changeover portion 11；Described body epimere 10 is cylindrical structure, on body
A circle lower convexity 101 is provided with 10 inwalls of section；Described changeover portion 11 is made up of smooth flat；Described body hypomere 12 is
Rectangular parallelepiped structure.
In order to ensure that the liquid in experiment process middle tube body 1 will not be excessive, ensure the precision and effect of detection, avoid surveying
Directional error is measured, described upper lid 2 includes a hollow circuit cylinder 20, the external diameter of hollow circuit cylinder 20 and the internal diameter phase of body epimere 10
Together, the outer wall of hollow circuit cylinder 20 be provided with that a circle matches with lower convexity 101 upper raised 201；Hollow circuit cylinder 20 is inserted into body
Inside epimere 10, upper raised 201 and lower convexity 101 cooperate snap connection, sealing of the lid 2 to body 1 in realization.
Testing liquid is added inside body 1 in order to facilitate testing crew, while meets the few liquid measure to be measured inspection in part
Survey demand, the diameter of body epimere 10 are more than the bottom side length of body hypomere 12.
Applicant obtains growing between 3.8-4.2mm when described body hypomere, body hypomere by constantly exploring
Width between 2.8-3.2mm, body hypomere height between 6.8-7.2mm, disclosure satisfy that more than 90% detection demand.
As shown in figure 4, in order to which suitable for existing detection device, the reaction tube connects formula layout using eight and set.
Contrast experiment：
1st, experimental principle
In practical application, test equipment uses the principle of fluorogenic quantitative detection, by adding fluorescent base in reaction system
Group, whole reaction process is monitored in real time using fluorescence signal accumulation, unknown template is quantitatively divided finally by standard curve
Analysis.The experiment is contrast test, and fluorescence signal is produced with ordinary conical shaped reaction pipe by analyzing the flat PCR reaction tubes of high printing opacity
The raw power influenceed, to determine the two good and bad characteristic in actual use.
2nd, process is tested
Test temperature：Room temperature；
Method of testing：As shown in fig. 7, in figure, 71 be supply lines for the device that is used in test process, and 72 be LED light source, 73
It is signal collecting element for supporing shell, 74,75 be signal processing unit, and 76 be reaction tube standing groove.After items are ready,
The flat PCR reaction tubes of high printing opacity and ordinary conical shaped reaction pipe are positioned in reaction tube standing groove 76 respectively, then mould will be tested
Type is placed in light tight space（Avoid influence of the extraneous light to experiment）Turning circuit, light caused by LED light source pass through reaction
Pipe, by the acquisition and recording experimental data of signal collecting element 74 and is delivered to signal processing unit through the light of reaction tube afterwards
75, by the analyzing and processing data of signal processing unit 75, and export test result.
3rd, experimental data
3.1st, test parameters
3.2nd, test data analyzer
A, two kinds of reaction tubes have a certain degree of weakening to light；
B, for any same medium, it is anti-that weakening degree of the conical reaction tube to light is all higher than the flat PCR of high printing opacity
Should weakening degree of the pipe to pipeline.
4th, conclusion
The high flat PCR reaction tubes of printing opacity have more excellent light transmission performance than plain taper bottom reaction tube, are more suitable for applying
During actually detected.
Finally it should be noted that：Obviously, above-described embodiment is only intended to clearly illustrate the utility model example,
And the restriction not to embodiment.For those of ordinary skill in the field, may be used also on the basis of the above description
To make other changes in different forms.There is no necessity and possibility to exhaust all the enbodiments.And thus
Among the obvious changes or variations amplified out is still in the scope of protection of the utility model.
Claims (3)
1. a kind of flat PCR reaction tubes of high printing opacity, including body and the upper lid being articulated with the side of body top, body is using folding
The environment-friendly materials that rate is small, transparency is high are penetrated to be made, it is characterised in that：
Described body is followed successively by body epimere, changeover portion and body hypomere from top to bottom, and body epimere and body hypomere pass through
Changeover portion connects；
Described body epimere is cylindrical structure, and a circle lower convexity is provided with body epimere inwall；
Described changeover portion is made up of smooth flat；
Described body hypomere is rectangular parallelepiped structure；
Described upper lid includes a hollow circuit cylinder, and the external diameter of hollow circuit cylinder is identical with the internal diameter of body epimere, outside hollow circuit cylinder
Wall is provided with the upper projection that a circle matches with lower convexity；Hollow circuit cylinder is inserted into intersegmental part on body, upper raised and lower convexity
Mutual cooperation snaps connection, sealing of the lid to body in realization.
2. a kind of high flat PCR reaction tubes of printing opacity as claimed in claim 1, it is characterised in that the diameter of body epimere is more than
The bottom side length of body hypomere.
A kind of 3. high flat PCR reaction tubes of printing opacity as claimed in claim 2, it is characterised in that the length of described body hypomere
Between 3.8-4.2mm, the width of body hypomere is between 2.8-3.2mm, and the height of body hypomere is between 6.8-7.2mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720413168.4U CN206692647U (en) | 2017-04-19 | 2017-04-19 | A kind of flat PCR reaction tubes of high printing opacity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720413168.4U CN206692647U (en) | 2017-04-19 | 2017-04-19 | A kind of flat PCR reaction tubes of high printing opacity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206692647U true CN206692647U (en) | 2017-12-01 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720413168.4U Expired - Fee Related CN206692647U (en) | 2017-04-19 | 2017-04-19 | A kind of flat PCR reaction tubes of high printing opacity |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206692647U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106906130A (en) * | 2017-04-19 | 2017-06-30 | 北京倍肯创新诊断技术研究院有限责任公司 | A kind of flat PCR reaction tubes of printing opacity high |
-
2017
- 2017-04-19 CN CN201720413168.4U patent/CN206692647U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106906130A (en) * | 2017-04-19 | 2017-06-30 | 北京倍肯创新诊断技术研究院有限责任公司 | A kind of flat PCR reaction tubes of printing opacity high |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171201 |