CN106885888A - The system that water quality toxicity monitoring and warning is carried out using zebra fish - Google Patents

The system that water quality toxicity monitoring and warning is carried out using zebra fish Download PDF

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Publication number
CN106885888A
CN106885888A CN201710185687.4A CN201710185687A CN106885888A CN 106885888 A CN106885888 A CN 106885888A CN 201710185687 A CN201710185687 A CN 201710185687A CN 106885888 A CN106885888 A CN 106885888A
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China
Prior art keywords
zebra fish
sedimentation basin
fish
water quality
warning
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Pending
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CN201710185687.4A
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Chinese (zh)
Inventor
许锐杰
蓝文林
杨金兰
黄振中
李丽华
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Guangdong Bytest Detection Technology Co Ltd
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Guangdong Bytest Detection Technology Co Ltd
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Priority to CN201710185687.4A priority Critical patent/CN106885888A/en
Publication of CN106885888A publication Critical patent/CN106885888A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/18Water
    • G01N33/186Water using one or more living organisms, e.g. a fish

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Separation Of Suspended Particles By Flocculating Agents (AREA)

Abstract

The invention discloses the system that water quality toxicity monitoring and warning is carried out using zebra fish, including the first sedimentation basin, the second sedimentation basin, the 3rd sedimentation basin, detection cell, zebra fish, this carries out the system of water quality toxicity monitoring and warning using zebra fish, can be by the animation characteristic reaction to zebra fish, the detection architecture to detecting water is set up in time, accurate monitoring efficiently monitoring is realized, complete biological water quality Toxicity Monitoring early warning system is effectively set up.

Description

The system that water quality toxicity monitoring and warning is carried out using zebra fish
Technical field
The present invention relates to water quality monitoring field, more particularly to the system that water quality toxicity monitoring and warning is carried out using zebra fish.
Background technology
Biological monitoring is usually used in the monitoring of industrial wastewater discharge and drinking water source water quality.Organism early warning system is using life Thing is continued to monitor, the system of early warning water pollution as sensing objects, is not set up still currently with organism early warning system complete Sound detection architecture can not effectively enable this system popularization and application, in view of disadvantages described above, is necessary that design is utilized in fact The system that zebra fish carries out water quality toxicity monitoring and warning.
The content of the invention
The technical problems to be solved by the invention are:There is provided and be using what zebra fish carried out water quality toxicity monitoring and warning Unite to solve the problems, such as that above-mentioned background technology is proposed.
In order to solve the above technical problems, the technical scheme is that:Water quality toxicity monitoring and warning is carried out using zebra fish System, including the first sedimentation basin, the second sedimentation basin, the 3rd sedimentation basin, detection cell, zebra fish.
Further, described operating procedure is as follows:
(1) swash plate mechanism, is installed in the first sedimentation basin, the second sedimentation basin, the 3rd sedimentation basin bottom respectively;
(2) source water persistently, is filled with the first sedimentation basin, the second precipitation can be escaped towards by the water of the sedimentation basin of principle of overflow first Pond, the water of the second sedimentation basin can escape towards the 3rd sedimentation basin;
(3), precipitated by three road swash plate mechanisms filtering silt, clear water enters detection cell;
(4), zebra fish is positioned over inside detection cell;
(5), reaction of the monitoring zebra fish inside detection cell;
(6) sampling of water quality is analyzed in time when, adverse reaction occurs in zebra fish;
(7) water quality conclusion, must be located according to sampling analysis result;
(8), every morning and afternoon (9:00,16:00) feed each 1 time, each feeding volume is eaten within 5 minutes with zebra fish It is complete to be advisable.Daily (9:30) 1 residual bait and fish faeces are cleared up;
(9), test fish has the lesion situation to pull cleaning out in time, and fish jar is carried out disinfection.Instrument and fish jar Gao Meng Sour potassium solution sterilization;
(10) replacing of detection cell test fish phase, 2-4 weeks replacement cycle.Able-bodied, euthesia the individual supply detection of selection Pond uses.
Further, in described operating procedure (4), the mantissa that zebra fish is placed is 20-30 bars.
Further, in described operating procedure (5), the content for monitoring zebra fish is:Breeding spawning, mutually residual situation, fish Ingest, bunch.
Further, in described operating procedure (6), the content of zebra fish adverse reaction is:There is dull, tail in indivedual fishes Sagging, phenomenon of being hovered in water.
Compared with prior art, the system that this carries out water quality toxicity monitoring and warning using zebra fish, can be by zebra fish Animation characteristic reaction, the detection architecture to detecting water is set up in time, realize accurate monitoring efficiently monitoring, effectively build Erect complete biological water quality Toxicity Monitoring early warning system.
Brief description of the drawings
Fig. 1 is the flow chart of the system that water quality toxicity monitoring and warning is carried out using zebra fish
Following specific embodiment will be further illustrated with reference to above-mentioned accompanying drawing.
Specific embodiment
Hereinafter, various specific details are elaborated, to provide the saturating of the concept to constituting described embodiment basis Thorough understanding.However, it will be apparent to those skilled in the art that described embodiment can be without these specific details In it is some or all in the case of put into practice.In other cases, without the well-known process step of specific descriptions.
As shown in figure 1, the system that water quality toxicity monitoring and warning is carried out using zebra fish, including the first sedimentation basin, second sink Shallow lake pond, the 3rd sedimentation basin, detection cell, zebra fish,
Described operating procedure is as follows:
(1) swash plate mechanism, is installed in the first sedimentation basin, the second sedimentation basin, the 3rd sedimentation basin bottom respectively;
(2) source water persistently, is filled with the first sedimentation basin, the second precipitation can be escaped towards by the water of the sedimentation basin of principle of overflow first Pond, the water of the second sedimentation basin can escape towards the 3rd sedimentation basin;
(3), precipitated by three road swash plate mechanisms filtering silt, clear water enters detection cell;
(4), zebra fish is positioned over inside detection cell;
(5), reaction of the monitoring zebra fish inside detection cell;
(6) sampling of water quality is analyzed in time when, adverse reaction occurs in zebra fish;
(7) water quality conclusion, must be located according to sampling analysis result;
(8), every morning and afternoon (9:00,16:00) feed each 1 time, each feeding volume is eaten within 5 minutes with zebra fish It is complete to be advisable.Daily (9:30) 1 residual bait and fish faeces are cleared up;
(9), test fish has the lesion situation to pull cleaning out in time, and fish jar is carried out disinfection.Instrument and fish jar Gao Meng Sour potassium solution sterilization;
(10) replacing of detection cell test fish phase, 2-4 weeks replacement cycle.Able-bodied, euthesia the individual supply detection of selection Pond uses.
The mantissa that zebra fish is placed is 20-30 bars.
Monitor zebra fish content be:Breed spawning, mutually residual situation, fish, bunch.
The content of zebra fish adverse reaction is:The dull, tail of indivedual fishes appearance is sagging, phenomenon of being hovered in water.The utilization The system that zebra fish carries out water quality toxicity monitoring and warning, can be by the animation characteristic reaction to zebra fish, and it is right to set up in time The detection architecture of water is detected, accurate monitoring efficiently monitoring is realized, complete biological water quality Toxicity Monitoring is effectively set up pre- Alert system.
First sedimentation basin, the second sedimentation basin, the swash plate mechanism of the 3rd sedimentation basin bottom are:Sedimentation basin bottom is equipped with two Swash plate, silt sinks to bottom after being acted on through swash plate, supernatant is acted on by overflow and enters into subordinate's sedimentation basin, the first sedimentation basin gear The a height of 60cm of plate, the second sedimentation basin baffle plate a height of 58cm, the 3rd a height of 55cm of sedimentation basin baffle plate, source water by after precipitation, Detection cell is overflowed to using the limpid raw water of the water level official post of sedimentation basins at different levels.
The present invention is not limited to above-mentioned specific embodiment, one of ordinary skill in the art from above-mentioned design, Without performing creative labour, a variety of conversion made are within the scope of the present invention.

Claims (5)

1. the system that water quality toxicity monitoring and warning is carried out using zebra fish, it is characterised in that including the first sedimentation basin, the second precipitation Pond, the 3rd sedimentation basin, detection cell, zebra fish.
2. the system for water quality toxicity monitoring and warning being carried out using zebra fish as claimed in claim 1, it is characterised in that described Operating procedure is as follows:
(1), the first sedimentation basin, the second sedimentation basin, the 3rd sedimentation basin bottom are provided with swash plate mechanism;
(2) source water persistently, is filled with the first sedimentation basin, the second sedimentation basin can be escaped towards by the water of the sedimentation basin of principle of overflow first, The water of the second sedimentation basin can escape towards the 3rd sedimentation basin;
(3), precipitated by three road swash plate mechanisms filtering silt, clear water enters detection cell;
(4), zebra fish is positioned over inside detection cell;
(5), reaction of the monitoring zebra fish inside detection cell;
(6) sampling of water quality is analyzed in time when, adverse reaction occurs in zebra fish;
(7) water quality conclusion, must be located according to sampling analysis result;
(8), every morning and afternoon (9:00,16:00) feed each 1 time, each feeding volume eaten up within 5 minutes with zebra fish for Preferably.Daily (9:30) 1 residual bait and fish faeces are cleared up;
(9), test fish has the lesion situation to pull cleaning out in time, and fish jar is carried out disinfection.Instrument and fish jar potassium permanganate Solution disinfection;
(10) replacing of detection cell test fish phase, 2-4 weeks replacement cycle.Able-bodied, euthesia the individual supply detection cell of selection makes With.
3. the system for water quality toxicity monitoring and warning being carried out using zebra fish as claimed in claim 2, it is characterised in that described In operating procedure (4), the mantissa that zebra fish is placed is 20-30 bars.
4. the system for water quality toxicity monitoring and warning being carried out using zebra fish as claimed in claim 2, it is characterised in that described In operating procedure (5), the content for monitoring zebra fish is:Breed spawning, mutually residual situation, fish, bunch.
5. the system for water quality toxicity monitoring and warning being carried out using zebra fish as claimed in claim 2, it is characterised in that described In operating procedure (6), the content of zebra fish adverse reaction is:The dull, tail of indivedual fishes appearance is sagging, phenomenon of being hovered in water.
CN201710185687.4A 2017-03-26 2017-03-26 The system that water quality toxicity monitoring and warning is carried out using zebra fish Pending CN106885888A (en)

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CN201710185687.4A CN106885888A (en) 2017-03-26 2017-03-26 The system that water quality toxicity monitoring and warning is carried out using zebra fish

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CN201710185687.4A CN106885888A (en) 2017-03-26 2017-03-26 The system that water quality toxicity monitoring and warning is carried out using zebra fish

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107651740A (en) * 2017-11-16 2018-02-02 房县慧泉水业有限公司 Drinking water process biotic warning method
CN108169440A (en) * 2017-12-14 2018-06-15 浙江海洋大学 A kind of new water pollution organism monitoring method
CN109061069A (en) * 2018-08-29 2018-12-21 华南农业大学 A kind of snail and fish eco-toxicity in-situ monitoring device and detection method
CN111999456A (en) * 2020-08-11 2020-11-27 莱西市鑫喆工程技术服务中心 Emergency biological early-warning type water quality monitoring method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103499675A (en) * 2013-09-30 2014-01-08 苏州国环环境检测有限公司 Method for monitoring toxicity of domestic sewage through zebra fishes
CN204151206U (en) * 2014-10-16 2015-02-11 山东省环境保护科学研究设计院 A kind of Simple treatment device of domestic sewage in rural areas

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
CN103499675A (en) * 2013-09-30 2014-01-08 苏州国环环境检测有限公司 Method for monitoring toxicity of domestic sewage through zebra fishes
CN204151206U (en) * 2014-10-16 2015-02-11 山东省环境保护科学研究设计院 A kind of Simple treatment device of domestic sewage in rural areas

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Title
周绍辉等: "基于斑马鱼行为变化的苯酚监测研究", 《安徽农业科学》 *
李倦生等: "《环境工程基础》", 1 August 2009 *
黄东龙等: "基于视觉技术的水质安全在线生物预警系统设计", 《中国给水排水》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107651740A (en) * 2017-11-16 2018-02-02 房县慧泉水业有限公司 Drinking water process biotic warning method
CN108169440A (en) * 2017-12-14 2018-06-15 浙江海洋大学 A kind of new water pollution organism monitoring method
CN108169440B (en) * 2017-12-14 2020-11-10 浙江海洋大学 Water pollution biological monitoring method
CN109061069A (en) * 2018-08-29 2018-12-21 华南农业大学 A kind of snail and fish eco-toxicity in-situ monitoring device and detection method
CN109061069B (en) * 2018-08-29 2021-08-24 华南农业大学 In-situ monitoring device and detection method for ecological toxicity of snails and fishes
CN111999456A (en) * 2020-08-11 2020-11-27 莱西市鑫喆工程技术服务中心 Emergency biological early-warning type water quality monitoring method

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Application publication date: 20170623