CN106885722A - 一种农作物干物质磨样方法 - Google Patents

一种农作物干物质磨样方法 Download PDF

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Publication number
CN106885722A
CN106885722A CN201710087238.6A CN201710087238A CN106885722A CN 106885722 A CN106885722 A CN 106885722A CN 201710087238 A CN201710087238 A CN 201710087238A CN 106885722 A CN106885722 A CN 106885722A
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China
Prior art keywords
dry
grind away
crops
sample
liquid nitrogen
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Inventor
黄群
齐海坤
严根土
王宁
徐庆华
乔文青
周红
石建斌
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Institute of Cotton Research of Chinese Academy of Agricultural Sciences
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Institute of Cotton Research of Chinese Academy of Agricultural Sciences
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Priority to CN201710087238.6A priority Critical patent/CN106885722A/zh
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2866Grinding or homogeneising

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

本发明涉及一种农作物样品制备方法,具体为农作物干物质磨样方法,该磨样方法是将烘干的棉花叶片、茎秆、根等物质减碎放入研钵,加入适量液氮迅速研磨成粉状,收集样品即可。本发明的研磨方法具有较强的实用性和可操作性,且省时省材、解决了少量干物质样本粉碎收集难的问题。

Description

一种农作物干物质磨样方法
技术领域
本发明涉及一种农作物样品制备方法,具体为农作物干物质磨样方法。
背景技术
在棉花等农作物育种工作中,研究元素含量对明确植物生理途径反应至关重要。植物元素含量测定通常需要将烘干后的植株粉碎过筛,用原子吸收分光光度计等设备测定,例如:红外线反射法、凯氏定氮法、直接蒸馏法、索氏提取法等。
目前,磨制干物质样品通常使用小型粉碎机(旋风式样品磨),采用超高速单相电机作为动力,根据刀片高速旋转,粉碎各种软硬药材。但用粉碎机粉碎物投入量通常大于4g,粉碎后的样品在刀片高速旋转过程中极易粘黏到粉碎机上,对于样品少的材料不适用。且对于潮湿和带油脂物品材料磨碎效果不是很好,也不能长时间连续工作,每次不能越过5min,加工数量多时,中间必须要休息,否者电机轴承过热造成电机损坏。
实验室种植材料往往数量较小,粉碎机研磨干物质对材料损失量大,对于研究元素含量带来不便,因此急需一种少量棉花等农作物干物质磨样方法、来解决样品少的问题。
发明内容
发明本发明的目的是针对上述不足,以棉花等农作物干物质磨样与收集更加方便快捷为出发点而设计。
实现本方法的技术方案是:
本发明的目的是提供一种农作物干物质磨样方法。
一种农作物干物质磨样方法,包括下述步骤:
(1)用酒精灼烧研钵、钵杵,进行灭菌;
(2)将磨样的农作物干物质剪碎,放入研钵;
(3)加入适量的液氮,使干农作物物质充分浸入液氮中;
(4)待液氮即将挥发完时,用钵杵迅速将农作物干物质研磨成粉末;
(5)收集样品。
本发明提供的一种农作物干物质磨样方法,具有较强的实用性和可操作性,且对材料损失量小;本发明的方法可以为收集少量植株干物质含量提供便利,减少珍贵样品的损失,解决了少量干物质样本粉碎收集难的问题。
该方法对棉花干物质研磨的良好效果,也可用于对玉米、小麦、水稻等作物叶片、茎干、根部位干物质的研磨实验。
具体实施方式
下面给出实施例,对本发明作进一步描述。
采用棉花为例子:
一种棉花干物质磨样方法,包括下述步骤:
(1)用酒精灼烧研钵、钵杵,进行灭菌;
(2)将磨样的棉花干物质剪碎,放入研钵;
(3)加入适量的液氮,使干棉花物质充分浸入液氮中;
(4)待液氮即将挥发完时,用钵杵迅速将棉花干物质研磨成粉末;
(5)收集样品。
将烘干的棉花干物质材料用此方法研磨,研磨耗时是用小型粉碎机的二分之一,最小研磨量可低于0.1g,且研磨0.1g样品的收集量达到92%,清洗磨样设备简单易行,解决多样研磨材料混杂的问题。

Claims (1)

1.一种农作物干物质磨样方法,其特征在于,包括下述步骤:
(1)用酒精灼烧研钵、钵杵,进行灭菌;
(2)将磨样的农作物干物质剪碎,放入研钵;
(3)加入适量的液氮,使干农作物物质充分浸入液氮中;
(4)待液氮即将挥发完时,用钵杵迅速将农作物干物质研磨成粉末;
(5)收集样品。
CN201710087238.6A 2017-02-17 2017-02-17 一种农作物干物质磨样方法 Pending CN106885722A (zh)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4776173A (en) * 1985-12-20 1988-10-11 Angio-Medical Corporation Method for extracting a substance from animal derived material
CN101650274A (zh) * 2009-08-31 2010-02-17 宁波新芝生物科技股份有限公司 一种高通量组织研磨机及研磨方法
CN102896033A (zh) * 2012-08-22 2013-01-30 王丽华 一种高通量植物叶片粉碎器的使用方法
CN105772196A (zh) * 2016-02-05 2016-07-20 平利县福音生物科技有限公司 一种麦冬的粉碎方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4776173A (en) * 1985-12-20 1988-10-11 Angio-Medical Corporation Method for extracting a substance from animal derived material
CN101650274A (zh) * 2009-08-31 2010-02-17 宁波新芝生物科技股份有限公司 一种高通量组织研磨机及研磨方法
CN102896033A (zh) * 2012-08-22 2013-01-30 王丽华 一种高通量植物叶片粉碎器的使用方法
CN105772196A (zh) * 2016-02-05 2016-07-20 平利县福音生物科技有限公司 一种麦冬的粉碎方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张译颖: "利用RNAi技术培育抗黑条矮缩病的水稻新品种", 《中国优秀硕士学位论文全文数据库 农业科技辑》 *

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