CN106872244B - A kind of method of the quick film-making of Chinese Hibiscu fiber - Google Patents
A kind of method of the quick film-making of Chinese Hibiscu fiber Download PDFInfo
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- CN106872244B CN106872244B CN201710072683.5A CN201710072683A CN106872244B CN 106872244 B CN106872244 B CN 106872244B CN 201710072683 A CN201710072683 A CN 201710072683A CN 106872244 B CN106872244 B CN 106872244B
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- fiber
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- mixed liquor
- mass percent
- bark
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- 239000000835 fiber Substances 0.000 title claims abstract description 65
- 238000000034 method Methods 0.000 title claims abstract description 23
- 244000284380 Hibiscus rosa sinensis Species 0.000 title claims abstract description 21
- 235000000100 Hibiscus rosa sinensis Nutrition 0.000 title claims abstract description 20
- 241000196324 Embryophyta Species 0.000 claims abstract description 36
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 27
- 229910017604 nitric acid Inorganic materials 0.000 claims abstract description 14
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 13
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims abstract description 13
- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 claims abstract description 11
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 15
- 238000004043 dyeing Methods 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 239000000975 dye Substances 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 5
- 239000008399 tap water Substances 0.000 claims description 5
- 235000020679 tap water Nutrition 0.000 claims description 5
- 238000002955 isolation Methods 0.000 abstract description 5
- 210000002421 cell wall Anatomy 0.000 abstract description 4
- 230000003287 optical effect Effects 0.000 abstract description 2
- 229960000583 acetic acid Drugs 0.000 abstract 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 abstract 1
- 238000010306 acid treatment Methods 0.000 abstract 1
- 238000009835 boiling Methods 0.000 abstract 1
- 239000012153 distilled water Substances 0.000 abstract 1
- 239000012362 glacial acetic acid Substances 0.000 abstract 1
- 229910052739 hydrogen Inorganic materials 0.000 abstract 1
- 239000001257 hydrogen Substances 0.000 abstract 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 abstract 1
- 229910052700 potassium Inorganic materials 0.000 abstract 1
- 239000011591 potassium Substances 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000005352 clarification Methods 0.000 description 3
- QLOKJRIVRGCVIM-UHFFFAOYSA-N 1-[(4-methylsulfanylphenyl)methyl]piperazine Chemical compound C1=CC(SC)=CC=C1CN1CCNCC1 QLOKJRIVRGCVIM-UHFFFAOYSA-N 0.000 description 2
- 238000010129 solution processing Methods 0.000 description 2
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000000879 optical micrograph Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
Abstract
A kind of method of the quick film-making of Chinese Hibiscu fiber, include the following steps: the fresh plant bark that will be peeled, it is first that 15%-25% potassium bichromate and 8%-15% nitric acid mixed liquor boil processing with mass percent, adding mass percent is that 10%-15% hydrogen peroxide and 50%-65% acetic acid mixed liquor boil boiling processing, bark after processing is put into distilled water and chooses plant fiber, is finally observed with sarranine stained preparation.The present invention is by conjunction with hydrogen peroxide-glacial acetic acid facture, quickly obtaining plant fiber for potassium bichromate-nitric acid treatment method.The isolation process greatly shortens in time than original method, and the fibre structure of acquisition is complete, can observe the structures such as clearly cell wall and pit under an optical microscope.
Description
Technical field
The invention belongs to the technical fields of the non-sliced film-making of biology, are related to a kind of method of quick film-making of Chinese Hibiscu fiber.
Background technique
Plant fiber is prevalent in the nutrition organs of plant, is a part of plant mechanical tissue, is also simultaneously
The important component part of plant transportation system is plant material transport, the main place for maintaining normal activities.Plant fiber
With multiple types, plant fiber can be divided into according to the position where fiber by wood-fibred, hypodermal fiber, bast fiber, skin
Layer fiber, pericyclic fiber, ring marrow fiber；Fiber can be divided into brilliant sheath fiber, embedding crystalline substance according to the particular tissues form of fiber
Fiber, crystal fibre, septate fibre, branched fiber etc..And usually plant fiber described in people is primarily referred to as wood-fibred, wood is fine
Dimension includes all elongated sclerenchyma in xylem in addition to conduit, parenchyma cell and tracheid.It is mainly contained in corpus fibrosum
Cellulose.But bast fiber is also component part important in plant fiber.Bast fiber is easier to isolate than wood-fibred
Come, therefore have great importance for observation of plant fibre structure, is the important materials source of fiber experimental teaching.
And it is domestic at present for the research of fiber be concentrated mainly on research to silk goods and for fiber optical activity,
It is studied the research especially for tropical fiber and has become a kind of recent studies on direction in the directions such as color performance.Handle fiber
Main method has alkaline process, acid system.Wherein alkaline process is a kind of ancient fiber-treating method, while being also most common side
Method, alkaline process processing, which can remove fatty acid in fiber and tartaric acid, branches away plant fiber, moreover it is possible to retain lignin.But
The currently used method isolation time is very long, and destructible fibre structure causes production effect bad.
In terms of teaching, Chinese Hibiscu fiber is typical common used material.Currently, the method for isolation Chinese Hibiscu fiber, main to use
10% potassium bichromate and 10% nitric acid segregation process, but this method is used, the time that when isolation is expended is long, and the fiber knot obtained
Structure is imperfect, is difficult to observe its structure such as clearly cell wall and pit structure under biomicroscope.
Summary of the invention
The object of the present invention is to provide a kind of method of quick film-making of Chinese Hibiscu plant fiber, this method isolation time is short, obtains
The fibre structure obtained is complete, and the structures such as clearly cell wall and pit can be observed under fibrescope.
The technical scheme is that a kind of method of the quick film-making of Chinese Hibiscu fiber, comprising the following steps:
(1), with scissors clip 2 years or more old branch items, bark is peeled, is dipped into mass percent as 15%-25% weight
In potassium chromate and 8%-15% nitric acid mixed liquor, 60~90min is boiled, then adding mass percent is 10%-15% mistake
Hydrogen oxide and 50%-65% acetic acid 10~20ml of mixed liquor, continue to boil 30~60min of processing；
(2), the plant tissue handled well is clipped in the culture dish equipped with tap water with tweezers, chooses plant with dissecting needle
Fiber is placed in clean glass slide, and the mass percent for instilling 0.5~1ml is that 0.1% sarranine dyeing liquor dyes 10min, so
The sarranine dyeing liquor extra with 70% alcohol wash clean afterwards, then the glycerol of 0.5~1ml50% is instilled, covered completes envelope
Piece；
(3), observation of plant fibre structure under biomicroscope.
Step (1) is to be handled that plant bark is made to be hardened and then slowly add with the mixed liquor of the potassium bichromate and nitric acid
Enter the acetic acid and hydrogen peroxide solution processing a period of time, obtains complete plant fiber.
Unless otherwise stated, percentage of the present invention is mass percent, and the sum of each component content percentage is
100%.
Inventor has found when handling Chinese Hibiscu bark with the mixed liquor of 10% potassium bichromate and 10% nitric acid, only with 10% weight
15~the 20h of time consumed required for potassium chromate and 10% nitric acid mixed liquor processing Chinese Hibiscu stem section bark, and obtained fiber
Observing effect it is poor, the fibre structure of part is decomposed, and the dyeing effect of cell wall is unintelligible.Then it is with Chinese Hibiscu in inventor
It finds make plant bark be hardened and then delay with the processing of the mixed liquor of potassium bichromate and nitric acid in the experiment that material is done again
The slow acetic acid and hydrogen peroxide solution processing a period of time of being added cracking can obtain complete plant fiber.
Detailed description of the invention
Fig. 1-1 is optical microphotograph microscopic observation Chinese Hibiscu fibrous structure chart.
Fig. 1-2 is Chinese Hibiscu fiber Local map.
Fig. 1-3 is Chinese Hibiscu fiber partial enlarged view (40x10).
Fig. 2-1 is Chinese Hibiscu fiber panorama sketch (100x).
Fig. 2-2 is Chinese Hibiscu fibre bundle Local map (100x).
Fig. 2-3 is Chinese Hibiscu fiber Local map (400x).
Fig. 2-4 is Chinese Hibiscu fiber Local map (400x).
Specific embodiment
Technical solution of the present invention is further detailed by the following examples.
Embodiment 1
With scissors clip 2 years or more Chinese Hibiscu old branch items, bark is peeled, it is 15% that bark, which is dipped into mass percent,
In potassium bichromate and 8% nitric acid mixed liquor, 60min is boiled, then adding mass percent is 10% hydrogen peroxide and 50%
Acetic acid mixed liquor 20ml continues to boil processing 30min.The plant tissue handled well is clipped to tweezers equipped with clarification tap water
In culture dish, choose plant fiber with dissecting needle, be placed in clean glass slide, instilling 0.5~1ml mass percent is
0.1% sarranine dyeing liquor dyes 10min.Then the sarranine dyeing liquor extra with 70% alcohol wash clean, then instill 0.5~
The glycerol of 1ml50%, covered complete mounting.Observed under electron microscope plant fiber structure.As a result such as Fig. 1-1, figure
1-2, Fig. 1-3.
Embodiment 2
With scissors clip 2 years or more Chinese Hibiscu old branch items, bark is peeled, it is 20% that bark, which is dipped into mass percent,
In potassium bichromate and 10% nitric acid mixed liquor, 90min is boiled, then adding mass percent is 12% hydrogen peroxide and 60%
Acetic acid mixed liquor 10ml continues to boil processing 30min.The plant tissue handled well is clipped to tweezers equipped with clarification tap water
In culture dish, choose plant fiber with dissecting needle, be placed in clean glass slide, instilling 0.5~1ml mass percent is
0.1% sarranine dyeing liquor dyes 10min.Then the sarranine dyeing liquor extra with 70% alcohol wash clean, then instill 0.5~
The glycerol of 1ml50%, covered complete mounting.Observation of plant fibre structure under biomicroscope.It is seen under biomicroscope
Examine plant fiber structure.It is not much different with the resulting result of the method and 1 acquired results of example.
Embodiment 3
With the old branch item of scissors clip Japan 2 years or more, bark is peeled, it is 25% that bark, which is dipped into mass percent,
In potassium bichromate and 15% nitric acid mixed liquor, boil 60~90min, then again by mass percent be 25% potassium bichromate and
15% nitric acid mixed liquor is outwelled, add mass percent be 15% hydrogen peroxide and 65% acetic acid 10~20ml of mixed liquor, after
It is continuous to boil 30~60min of processing.The plant tissue handled well is clipped in the culture dish equipped with clarification tap water with tweezers, with solution
It cuts open needle and chooses plant fiber, be placed in clean glass slide, instill 0.5~1ml mass percent as 0.1% sarranine dyeing liquor dye
Color 10min.Then the sarranine dyeing liquor extra with 70% alcohol wash clean, then the glycerol of 0.5~1ml50% is instilled, it closes the lid
Slide completes mounting.Observation of plant fibre structure under biomicroscope.With the resulting result of the method and 1 acquired results of example
It is not much different.Such as Fig. 2-1, Fig. 2-2, Fig. 2-3, Fig. 2-4.
Claims (1)
1. a kind of method of the quick film-making of Chinese Hibiscu fiber, which comprises the following steps:
(1), with scissors clip 2 years or more Japan's old branch items, bark is peeled, it is 15%- that bark, which is dipped into mass percent,
In 25% potassium bichromate and 8%-15% nitric acid mixed liquor, 60~90min is boiled, then adding mass percent is 10%-15% mistake
Hydrogen oxide and 50%-65% acetic acid 10~20ml of mixed liquor, continue to boil 30~60min of processing,
Being handled with the mixed liquor of the potassium bichromate and nitric acid makes plant bark be hardened and then be slowly added to the acetic acid and mistake
Hydrogen oxide mixed liquor processing a period of time, obtain complete plant fiber；
(2), the plant tissue handled well is clipped in the culture dish equipped with tap water with tweezers, chooses plant fibre with dissecting needle
Dimension, is placed in clean glass slide, and instilling 0.5~1ml mass percent is that 0.1% sarranine dyeing liquor dyes 10min, then uses
The extra sarranine dyeing liquor of 70% alcohol wash clean, then the glycerol of 0.5~1ml50% is instilled, covered completes mounting；
(3), observation of plant fibre structure under biomicroscope.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710072683.5A CN106872244B (en) | 2017-02-10 | 2017-02-10 | A kind of method of the quick film-making of Chinese Hibiscu fiber |
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| CN201710072683.5A CN106872244B (en) | 2017-02-10 | 2017-02-10 | A kind of method of the quick film-making of Chinese Hibiscu fiber |
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| CN106872244A CN106872244A (en) | 2017-06-20 |
| CN106872244B true CN106872244B (en) | 2019-02-05 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| SU1124197A1 (en) * | 1982-07-05 | 1984-11-15 | Ростовский-На-Дону Ордена Трудового Красного Знамени Государственный Университет Им.М.А.Суслова | Fluorescent dye for micro ornanisms and plant tissues |
| US7842465B2 (en) * | 2006-01-17 | 2010-11-30 | Palo Alto Research Center Incorporated | Immunocytostaining methods for enhanced dye ratio discrimination in rare event detection |
| CN102279116A (en) * | 2011-07-14 | 2011-12-14 | 北京大学第三医院 | Method for preparing membrane-type sample slice |
| JP5948791B2 (en) * | 2011-10-25 | 2016-07-06 | 三菱レイヨン株式会社 | Method for preparing cross-sectional sample of wet porous fiber |
| CN105716926B (en) * | 2014-12-02 | 2020-01-17 | 新疆农业大学 | Dyeing method of almond anther cell fiber skeleton |
| CN105954083B (en) * | 2016-03-01 | 2018-09-25 | 广西大学 | The method that tithonia blade epicuticle isolates film-making |
| CN106350593A (en) * | 2016-09-19 | 2017-01-25 | 长江师范学院 | Dripping piece preparation method of plant chromosome |
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