CN102279116A - Method for preparing membrane-type sample slice - Google Patents
Method for preparing membrane-type sample slice Download PDFInfo
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- CN102279116A CN102279116A CN 201110197074 CN201110197074A CN102279116A CN 102279116 A CN102279116 A CN 102279116A CN 201110197074 CN201110197074 CN 201110197074 CN 201110197074 A CN201110197074 A CN 201110197074A CN 102279116 A CN102279116 A CN 102279116A
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- cellulose acetate
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- membrane
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Abstract
The invention discloses a method for preparing a membrane-type sample slice. The method comprises the following steps of: spreading a sample to be treated on a cellulose acetate membrane and fixing the sample and the cellulose acetate membrane together; and integrally taking the sample and the cellulose acetate membrane out and performing dehydration, embedding and dyeing, wherein in the embedding step, the cellulose acetate membrane attached with the sample is vertically embedded in paraffin. By the method, a tissue sample having a clear and complete structure is obtained, and the slice preparing quality of the sample in the type is improved greatly. The method has a wide application prospect in the pathology detection field.
Description
Technical field
The present invention relates to a kind of method for preparing the section of film class sample.
Background technology
In the present pathology work, the sample of the less or film class of the volume of censorship, fixing back sample is very easily curling in formalin, and folding, sample is very easily crumbed in the organization embedding process, breaks, and pathological diagnosis and observation are made a big impact.
Summary of the invention
An object of the present invention is to provide a kind of method of the fixing sample that easily curls or easily fold.
The method of the fixing sample that easily curls or easily fold provided by the present invention comprises the steps: pending sample is spread on the cellulose acetate membrane, fixes together again.
In the said method, described cellulose acetate membrane is the ethane via epoxyethane sterilization.
In above-mentioned arbitrary described method, described sample is a Pathologic specimen.
In above-mentioned arbitrary described method, described Pathologic specimen is the tunica fibrosa that exsomatizes, stripped hyperplasia film or stripped laminar tissue (this type of laminar tissue mostly is the thin slice of the not enough 1mm of thickness greatly, and long or wide is 1-3mm).
Another object of the present invention provides a kind of method for preparing the paraffin section of the sample that easily curls or easily fold.
The method of the paraffin section of preparation provided by the invention sample easily curling or that easily fold comprises the steps: according to above-mentioned arbitrary described method pending sample to be fixed; Take out sample and cellulose acetate membrane are whole, the preparation paraffin section in the embedding step, is embedded in the paraffin the cellulose acetate membrane that is pasted with sample is upright.
The present inventor is through groping in a large number, searches out a kind of thin samples such as film class to be played a supportive role, and can better adapt to the material of Pathologic specimen handling procedure and section statining, be i.e. cellulose acetate membrane.Be attached at sample on this material and sample carries out organized processing, section and dyeing together, obtained clear in structure, complete tissue specimen, improved the slice-making quality of this type of sample greatly.In the whole operation process, sample does not bend or is folding, very smooth always, greatly facilitates operation and has saved the time.In the embedding process, can pinch and get cellulose acetate film and not direct contact tissue, the phenomenon of having avoided sample to be crumbed and break.In whole process, cellulose acetate membrane is stable in properties in alcohol, dimethylbenzene and paraffin, and has guaranteed the form of tissue.Cellulose acetate membrane keeps the height consistance with tissue in dehydration, transparent and section statining process, make tissue that good supporting surface arranged, and has made differentiation for the face of organizing of drawing materials when helping observing.Therefore, the inventive method has broad application prospects in the pathology detection field.
Description of drawings
Fig. 1 is the schematic flow sheet of the inventive method.
Fig. 2 is the section result of experimental group.
Fig. 3 is the section result of control group.
Embodiment
Employed experimental technique is conventional method if no special instructions among the following embodiment.
Used material, reagent etc. if no special instructions, all can obtain from commercial channels among the following embodiment.
Embodiment 1, the present invention prepare the method for Pathologic specimen section
The hyperplasia film of used sample for taking off in the operation.Fig. 2, Fig. 3 are amphiblestroid scar proliferation film after the eye wound.
The schematic flow sheet of the inventive method as shown in Figure 1.
One, the inventive method
The cellulose acetate membrane pre-service: cellulose acetate membrane is cut into 5mm * 5mm size box, and standby with packing behind the ethylene oxide sterilizing.
Fixing: pending sample is spread on the cellulose acetate membrane, need not differentiate the cellulose acetate membrane pros and cons, it is fixing to put into formalin fixed liquid after sprawling.
Finishing: the cellulose acetate membrane that will be pasted with sample is taken out from immobile liquid, according to the size that spreads over sample on the cellulose acetate membrane, unnecessary cellulose acetate membrane is cut, and makes residue cellulose acetate membrane shape identical substantially with the sample shape that attaches thereon.
Dehydration, transparent: conventional method is carried out.
Embedding: upright being embedded in the paraffin of cellulose acetate membrane that will be pasted with sample.
Section, dyeing: conventional method is carried out.
Two, contrast method
Directly place formalin fixed liquid fixing sample, dewater successively again, transparent, embedding, section, staining procedure.
Three, result
Experimental group: in fixation procedure, sample does not curl; In embedding and slicing processes, directly pinch and get cellulose acetate film and operate and directly do not contact sample, make sample that fragmentation not take place; The section that obtains at last is complete, can react really in vivo existence form of sample, growth pattern and and surrounding tissue between relation.For pathological diagnosis and scientific research provide complete information on morphology.Section such as Fig. 2 of obtaining.
Control group: in fixation procedure, sample take place to curl, and causes in embedding and slicing processes, and sample do not unfold, easily overlapping; In embedding and slicing processes, directly sample is operated, make sample easily be crumbed and break; Cause the slice information that obtains imperfect at last, but broken sample information, in addition since the overlapping some parts sample that causes can not be recognized, and lose sample information.Section such as Fig. 3 of obtaining.
Claims (5)
1. the method for a fixing sample that easily curls or easily fold comprises the steps: pending sample is spread on the cellulose acetate membrane, fixes together again.
2. method according to claim 1 is characterized in that: described cellulose acetate membrane is the ethane via epoxyethane sterilization.
3. method according to claim 1 and 2 is characterized in that: described sample is a Pathologic specimen.
4. according to arbitrary described method among the claim 1-3, it is characterized in that: described Pathologic specimen is the tunica fibrosa that exsomatizes, stripped hyperplasia film or stripped laminar tissue.
5. a method for preparing the paraffin section of the sample that easily curls or easily fold comprises the steps: according to arbitrary described method among the claim 1-4 pending sample to be fixed; Take out sample and cellulose acetate membrane are whole, the preparation paraffin section in the embedding step, is embedded in the paraffin the cellulose acetate membrane that is pasted with sample is upright.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110197074 CN102279116A (en) | 2011-07-14 | 2011-07-14 | Method for preparing membrane-type sample slice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110197074 CN102279116A (en) | 2011-07-14 | 2011-07-14 | Method for preparing membrane-type sample slice |
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| Publication Number | Publication Date |
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| CN102279116A true CN102279116A (en) | 2011-12-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201110197074 Pending CN102279116A (en) | 2011-07-14 | 2011-07-14 | Method for preparing membrane-type sample slice |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104596815A (en) * | 2014-12-17 | 2015-05-06 | 西南林业大学 | Bamboo sliding section staining production method |
| CN106872244A (en) * | 2017-02-10 | 2017-06-20 | 广西大学 | A kind of method of the quick film-making of Chinese Hibiscu fiber |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2449219Y (en) * | 2000-11-17 | 2001-09-19 | 西安交通大学第二医院 | Tissue chip preparing apparatus |
| CN2779376Y (en) * | 2004-08-09 | 2006-05-10 | 南开大学 | Plant surface replica memnrane |
| CN1912567A (en) * | 2006-07-10 | 2007-02-14 | 中国水产科学研究院黄海水产研究所 | Paraffin section method for seawater fish egg |
-
2011
- 2011-07-14 CN CN 201110197074 patent/CN102279116A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2449219Y (en) * | 2000-11-17 | 2001-09-19 | 西安交通大学第二医院 | Tissue chip preparing apparatus |
| CN2779376Y (en) * | 2004-08-09 | 2006-05-10 | 南开大学 | Plant surface replica memnrane |
| CN1912567A (en) * | 2006-07-10 | 2007-02-14 | 中国水产科学研究院黄海水产研究所 | Paraffin section method for seawater fish egg |
Non-Patent Citations (2)
| Title |
|---|
| 《口腔医学》 20100131 张金艳等 《大鼠全牙器官体外培养模型的建立》 第24-26页 1-5 第30卷, 第1期 * |
| 《现代口腔医学杂志》 20071130 朱晓茹等 《人牙齿切片器官培养模型的建立》 第600-602页 1-5 第21卷, 第6期 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104596815A (en) * | 2014-12-17 | 2015-05-06 | 西南林业大学 | Bamboo sliding section staining production method |
| CN106872244A (en) * | 2017-02-10 | 2017-06-20 | 广西大学 | A kind of method of the quick film-making of Chinese Hibiscu fiber |
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Application publication date: 20111214 |