SU1124197A1 - Fluorescent dye for micro ornanisms and plant tissues - Google Patents

Abstract

The use of perchlorate pyrimi / to | , 2-a J en-imidazole as a fluorescent dye for microorganisms and plant tissues.

Description

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This invention relates to the use of fluorescent dyes in the study of microorganisms and plant tissues, in particular, the use of fluorescent dyes.

Fluorescent dyes are known for microorganisms and plant tissues that have blue-green fluorescence. One of the best dyes is 2- (4-aminophenyl) -5- (2-benzthiazole1) benzthiazole (primulin) Cl3

The disadvantage of such a dye is its high working concentration (0.01-0.02%) and a narrow working pH range (7, since at pH 7 there are two types of fluorescence due to the different particle size of the particles).

Pyrimido 1,2-a benzimidazole perchlorate is a substance that is described in the literature as C2 chemical reagent. 7 The purpose of the invention is to reduce the working concentration of a fluorochrome solution and to extend the working pH range.

The goal is achieved by the use of pyrimido iieplochloride C 2 -aBenzimidazole as a fluorescent dye for microorganisms and plant tissues.

An example. Pyrimido-aZ benzimidazolium has the structural formula

R

..

|| 1 / -4 p G | H

R

R R- SdN 19R -

R-CfiHs R-CHj Compound (I) and (II) are used for plant tissues. A preparation of onion epidermis is prepared on a cover glass in a to & water pool, then 0.5-1.0 ml of a 0.0025% alcoholic solution of the compound is applied (I After 5-IO min. The solution is drained, the preparation is washed with distilled water or 25% ethyl alcohol. Microscopic in ultraviolet spectrum. The core and the shell give a bright blue-green fluorescence.

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PRI mme R 2. Prepare the epidermal aloe drug. Stain with compound (II) for 5-10 min. Washed with distilled water and microscoped in ultraviolet light. Cell membranes of the dermis and the stomata give bright blue non-green fluorescence (see tab.1).

The intensity of fluorescence during the dyeing of plant tissues with compounds (I) and (II) of pyrimido Cl, 2-a3 benzimidazole perchlorates is given in Table 1.

As can be seen from table 1, vegetable

tissues are stained in a dye concentration range of 0.0012 to 0.005%. At higher dye concentrations, its crystals precipitate.

Example 3: To stain the microorganisms, smears from the culture are prepared, dried over the flame of the burner. Then the smear is poured with 0.5-1 ml of 0.0025% alcoholic solution of compound (I). After 10 minutes, the dye solution is drained, washed with distilled water and microscoped in ultraviolet light. Microbial cells produce a bright blue-green fluorescence (see table 2).

The fluorescence intensity of Penicillium sp during the staining with compounds (I) and (II) of pyrimido l, 2-aJ benzimidazole perchlorates is given in Table. 2

Staining of microorganisms and plant cells is carried out at different pH of the dye solutions, which does not affect the quality of the fluorescence (pH from 3 to 9).

Thus, the use of pyrimido p, 2-a 1 benzimidazole perchlorate as a fluorochrome makes it possible to stain microorganisms and plant cells at lower (4-6 times in comparison with the prototype) cb concentrations, and also to conduct coloring in a wider pH range. solution, which greatly expands the scope of application of the dye. In addition, preparations stained with this dye do not lose intensity when stored.