CN106855515A - Collaurum quantitative fluorescence analysis all-in-one and its control method - Google Patents
Collaurum quantitative fluorescence analysis all-in-one and its control method Download PDFInfo
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- CN106855515A CN106855515A CN201710046763.3A CN201710046763A CN106855515A CN 106855515 A CN106855515 A CN 106855515A CN 201710046763 A CN201710046763 A CN 201710046763A CN 106855515 A CN106855515 A CN 106855515A
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- 238000000034 method Methods 0.000 title claims abstract description 21
- 238000012921 fluorescence analysis Methods 0.000 title claims abstract description 20
- 238000012360 testing method Methods 0.000 claims abstract description 204
- 238000010166 immunofluorescence Methods 0.000 claims abstract description 18
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 17
- 238000001514 detection method Methods 0.000 claims description 40
- 238000002347 injection Methods 0.000 claims description 32
- 239000007924 injection Substances 0.000 claims description 32
- 238000005070 sampling Methods 0.000 claims description 12
- 230000007306 turnover Effects 0.000 claims description 9
- 238000004458 analytical method Methods 0.000 claims description 8
- 238000005286 illumination Methods 0.000 claims description 8
- 230000003287 optical effect Effects 0.000 claims description 7
- 230000005622 photoelectricity Effects 0.000 claims description 2
- 238000003908 quality control method Methods 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 9
- 238000002474 experimental method Methods 0.000 abstract description 5
- 238000010185 immunofluorescence analysis Methods 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 description 7
- 238000006073 displacement reaction Methods 0.000 description 4
- 241001269238 Data Species 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
Abstract
The invention discloses collaurum quantitative fluorescence analysis all-in-one and its control method, the effect that the all-in-one passes through microprocessor and connected lighting unit, shooting unit, graphics processing unit, laser and the first photoelectric sensor, so that all-in-one of the invention can both work as colloidal gold chart reader and use, immunofluorescence analysis instrument can be worked as again to use, cost is saved.Present invention also offers a kind of control method of above-mentioned all-in-one so that operator need not distinguish test card for colloidal gold test paper card or immunofluorescence test card in use, reduce experimental procedure, improve conventional efficient.
Description
Technical field
The present invention relates to clinical detection technique field, in particular to a kind of collaurum quantitative fluorescence analysis all-in-one and its control
Method processed.
Background technology
Existing clinical testing laboratories would generally be entered using collaurum method or immunofluorescence method to various physicochemical datas
Row detection, common instrument has colloidal gold chart reader, immunofluorescence analysis instrument etc..But two kinds of instruments are combined into due to lacking one kind
The instrument of one, causes laboratory to be generally equipped with above two instrument, and the different test cards that above two instrument is directed to are needed
Want laboratory technician to mark to distinguish, be unfavorable for that conventional efficient is improved.
The content of the invention
Present invention aim to overcome existing clinical detection instrument single, it is impossible at the same using collaurum method or
A kind of problem that immunofluorescence method is detected to various physicochemical datas, there is provided collaurum quantitative fluorescence analysis all-in-one, it
The characteristics of with can be detected to various physicochemical datas using immunofluorescence method using collaurum method again.
To achieve the above object, the collaurum quantitative fluorescence analysis all-in-one designed by the present invention, including body and be located at
Turnover sample opening and display screen on the body；Microprocessor and the photograph being connected with the microprocessor are provided with the body
Bright unit, shooting unit, graphics processing unit, laser and the first photoelectric sensor；
The turnover sample opening is used to be put into or take out test card；
The lighting unit is used to export illumination light to the test card；
The shooting unit is used to carry out optical pickup to the test card and draw shooting result；
Described image processing unit is used to be analyzed treatment to the shooting result of the shooting unit, draws test card matter
Control line gray value and detection line gray value；
The laser is used to carry out laser irradiation to the test card, the test card is sent fluorescence；
First photoelectric sensor is used to receive the fluorescence and be processed as electric signal according to fluorescence intensity to send to institute
State microprocessor；
The microprocessor is by calculating the ratio of nature controlling line gray value and detection line gray value or processing first light
The electric signal of electric transducer draws testing result；
The display screen is used to show the testing result.
Further, it is additionally provided with the body and be connected with the microprocessor and institute is stretched out by the turnover sample opening
State the sampling device of body；The sampling device includes the test paper disk of the level for holding multiple test cards and for solid
The sample injection disc of the level of the fixed test paper disk；The test paper disk upper surface is provided with multiple cards with the test card corresponding matching
Groove；The sample introduction tray bottom is provided with slide rail, and sample injection disc side wall is provided with the tooth bar set along sidewall direction；It is additionally provided with the body
The gear coordinated with the tooth bar and the motor for driving the pinion rotation；The motor is connected with the microprocessor；It is described
Lighting unit, shooting unit, laser and the first photoelectric sensor are directly over the test paper disk.
The beneficial effect of above-mentioned further scheme is：It is placed in the draw-in groove of test paper disk by by multiple test cards so that one
Individual test paper disk can improve detection efficiency with stationary arrangement multiple test card；And by sample injection disc and tooth bar, the tooth of sample injection disc side wall
The effect of wheel and motor, with Automated condtrol sample introduction and can go out sample, and sample introduction stabilization, be not in test paper caused by manual operation
The problems such as card inclination is rocked.
Preferably, the second photoelectric sensor located at the sampling device both sides, the test paper are additionally provided with the body
Disk is provided with the raised catch for cutting off second photo-sensor signal, second photoelectricity in each described draw-in groove side
Sensor is connected with the microprocessor.
The beneficial effect of above-mentioned preferred scheme is：By the second photoelectric sensor and the positioning action of catch so that micro- place
Reason device effectively controlled motor can work, so as to limit each displacement of sample injection disc as two test paper in Test paper card
The distance between card, so as to often detect a test card, it is to be detected that sample injection disc reach once causes that next test card is in
Region.
Further, also including can be sticked on the test paper disk or on the test card for by the test card area
It is divided into the identification code of colloidal gold test paper card or immunofluorescence test card.
The beneficial effect of above-mentioned further scheme is：By the effect of identification code, may be such that all-in-one is automatic by test card
Colloidal gold test paper card or immunofluorescence test card are divided into, so that detected accordingly for the test card, it is less unnecessary
Experimental procedure, improve detection efficiency.
Preferably, the test paper disk and the removable connection of the sample injection disc.
The beneficial effect of above-mentioned preferred scheme is：By by test paper disk and the removable connection of sample injection disc so that test paper disk is torn open
After unloading, test card more convenient and quicker is placed.
Further, the test paper disk is provided with positioning through hole；The sample injection disc is provided with and coordinates with the positioning through hole
Alignment pin.
The beneficial effect of above-mentioned further scheme is：By the effect of alignment pin and positioning through hole, prevent sample injection disc from moving
Test paper disk is subjected to displacement with it during dynamic.
Preferably, the test card is colloidal gold test paper card or immunofluorescence test card.
The beneficial effects of the invention are as follows：By microprocessor and connected lighting unit, shooting unit, image procossing
The effect of unit, laser and the first photoelectric sensor so that all-in-one of the invention can both work as colloidal gold chart reader and use,
Immunofluorescence analysis instrument can be worked as again to use, cost is saved, and only need single injected sampling to can detect the examination that multiple scribbles sample
Paper card, is colloidal gold test paper card or immunofluorescence test card without test card is distinguished, and reduces experimental procedure, improves experiment
Efficiency.
The invention allows for a kind of control method of collaurum quantitative fluorescence analysis all-in-one, comprise the following steps：
Step 1) test card is put into body by passing in and out sample opening；
Step 2) test card enter lighting unit operating area, microprocessor control lighting unit it is defeated to the test card
Go out illumination light, and control the shooting unit to carry out optical pickup to the test card, draw shooting result；
Step 3) microprocessor control graphics processing unit to the step 2) shooting result be analyzed treatment,
When analysis processing result draws test card detection line gray value and nature controlling line gray value, according to nature controlling line gray scale
The ratio of both value, detection line gray value or calculating draws testing result, carries out step 7)；
When analysis processing result draws be not detected by test card nature controlling line gray value and detection line gray value, walked
It is rapid 4)；
Step 4) microprocessor control laser laser irradiation is carried out to the test card, the test card is sent glimmering
Light；
Step 5) the first photoelectric sensor receives the step 4) in fluorescence and be processed as electric signal and send to described micro-
Processor；
Step 6) step 5 described in microprocessor processes) described in electric signal draw fluorescent bands result；
When fluorescent bands result draws test card nature controlling line fluorescence intensity level and detection line fluorescence intensity level, according to Quality Control
The ratio of both line fluorescence intensity level, detection line fluorescence intensity level or calculating draws testing result；
Step 7) testing result feeds back to display screen and shown by microprocessor；
Step 8) return to step 2) carry out next test card detection.
The beneficial effects of the invention are as follows：Sample is detected by this method, without distinguishing test card for collaurum is tried
Paper card or immunofluorescence test card, testing result can all be shown by display screen, improve conventional efficient.
Brief description of the drawings
Fig. 1 is the dimensional structure diagram of collaurum quantitative fluorescence analysis all-in-one of the present invention.
Fig. 2 is the principle schematic of collaurum quantitative fluorescence analysis all-in-one of the present invention.
Fig. 3 is the structural representation that is installed on sample injection disc of test paper disk of collaurum quantitative fluorescence analysis all-in-one of the present invention
Figure.
Fig. 4 is the dimensional structure diagram of sample injection disc in Fig. 3.
Fig. 5 is the schematic top plan view of test paper disk in Fig. 3.
Fig. 6 be Fig. 5 in test paper disk remove the dimensional structure diagram after test card.
Fig. 7 is the schematic cross-sectional view in A-A faces in Fig. 6.
In figure, 1. body；2. sample opening is passed in and out；3. display screen；4. microprocessor；5. lighting unit；6. shooting unit；7.
Graphics processing unit；8. laser；9. the first photoelectric sensor；10. test card；11. test paper disks；12. sample injection discs；13. draw-in grooves；
14. tooth bars；15. gears；16. motors；17. second photoelectric sensors；18. catch；19. numberings；20. positioning through hole；21. positioning
Pin；22. sampling devices.
Specific embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in further detail.
Collaurum quantitative fluorescence analysis all-in-one as shown in Fig. 1~7, including body 1 and the turnover sample on body 1
Opening 2 and display screen 3；Microprocessor 4 and the lighting unit 5, shooting unit 6, the figure that are connected with microprocessor 4 are provided with body 1
As processing unit 7, the photoelectric sensor 9 of laser 8 and first；
Turnover sample opening 2 is used to be put into or take out test card 10；
Lighting unit 5 is used to export illumination light to test card 10；
Shooting unit 6 is used to carry out optical pickup to test card 10 and draw shooting result；
Graphics processing unit 7 is used to be analyzed treatment to the shooting result of shooting unit 6, draws the nature controlling line of test card 10
Gray value and detection line gray value；
Laser 8 is used to carry out laser irradiation to test card 10, test card 10 is sent fluorescence；
First photoelectric sensor 9 is used to receive fluorescence and be processed as electric signal according to fluorescence intensity to send to microprocessor 4；
Microprocessor 4 is by calculating the ratio of nature controlling line gray value and detection line gray value or processing the first photoelectric sensor
9 electric signal draws testing result；
Display screen 3 is used to show testing result.
The sampling device 22 for being connected with microprocessor 4 and body 1 being stretched out by passing in and out sample opening 2 is additionally provided with body 1；Enter
Sampling device 22 includes the test paper disk 11 of the level for holding multiple test cards 10 and enters for fixing the level of test paper disk 11
Sample dish 12；The upper surface of test paper disk 11 is provided with multiple draw-in grooves 13 with the corresponding matching of test card 10；The bottom of sample injection disc 12 is provided with slide rail
(not shown), the side wall of sample injection disc 12 is provided with the tooth bar 14 set along sidewall direction；The tooth coordinated with tooth bar 14 is additionally provided with body 1
The motor 16 that wheel 15 and drive gear 15 are rotated；Motor 16 is connected with microprocessor 4；Lighting unit 5, shooting unit 6, laser
8 and first photoelectric sensor 9 directly over test paper disk 11.By the draw-in groove 13 that multiple test cards 10 are placed on test paper disk 11
It is interior so that a test paper disk 11 can improve detection efficiency with stationary arrangement multiple test card 10；And by sample injection disc 12 and sample introduction
The effect of the tooth bar 14, gear 15 and motor 16 of the side wall of disk 12, with Automated condtrol sample introduction and can go out sample, and sample introduction stabilization, no
Test card 10 inclines the problems such as rocking caused by manual operation occurs.
The second photoelectric sensor 17 located at the both sides of sampling device 22 is additionally provided with body 1, test paper disk 11 is in each draw-in groove
13 sides are provided with the raised catch 18 for cutting off the signal of the second photoelectric sensor 17, the second photoelectric sensor 17 and microprocessor
4 connections.By the second photoelectric sensor 17 and the positioning action of catch 18 so that microprocessor 4 can effective controlled motor 16
Work, so as to limit each displacement of sample injection disc 12 as the distance between two test cards 10 in Test paper card 10,
So as to often detect a test card 10, sample injection disc 12 moves forward and once cause next test card 10 in region to be detected.
Also include can be sticked on test paper disk 11 or on test card 10 for test card 10 to be divided into colloid gold test paper
The identification code (not shown) of card or immunofluorescence test card.Identification code can be that Quick Response Code can also be coated with different colours
The scraps of paper；Laboratory technician would indicate that the identification code of colloidal gold test paper card is sticked in corresponding colloidal gold test paper card correspondence position, would indicate that
The identification code of immunofluorescence test card is sticked position corresponding in corresponding immunofluorescence test card.Shooting unit 6 is to test card
In 10 shooting process, also by identification code shot and be uploaded to graphics processing unit 7 process obtain the identification code correspondence
Information, microprocessor 4 judges that the test card 10 is colloidal gold test paper card or immunofluorescence test card by the information, so as to enter
Row corresponding operating.
In addition, the upper surface of test paper disk 11 is provided with the numbering 19 or mark for identifying the draw-in groove 13 in each side of draw-in groove 13
Sign.By numbering 19 or label that correspondence draw-in groove 13 is set on test paper disk 11, in that context it may be convenient to record corresponding test card 10
Data, prevent data entanglement.
Test paper disk 11 and the removable connection of sample injection disc 12.By by test paper disk 11 and the removable connection of sample injection disc 12 so that
After test paper disk 11 is dismantled, the more convenient and quicker of test card 10 is placed.
Test paper disk 11 is provided with positioning through hole 20；Sample injection disc 12 is provided with the alignment pin 21 coordinated with positioning through hole 20.It is logical
The effect of alignment pin 21 and positioning through hole 20 is crossed, prevents the test paper disk 11 in moving process of sample injection disc 12 to be subjected to displacement with it.
Test card 10 is colloidal gold test paper card or immunofluorescence test card.
By microprocessor 4 and connected lighting unit 5, shooting unit 6, graphics processing unit 7, the and of laser 8
The effect of the first photoelectric sensor 9 so that all-in-one of the invention can both work as colloidal gold chart reader and use, can work as immune again
Fluorescence analyser is used, and saves cost, and only needs single injected sampling to can detect the test card 10 that multiple scribbles sample, without
It is colloidal gold test paper card or immunofluorescence test card to distinguish test card 10, reduces experimental procedure, improves conventional efficient.
The invention allows for a kind of control method of collaurum quantitative fluorescence analysis all-in-one, comprise the following steps：
Step 1) test card 10 is put into body 1 by passing in and out sample opening 2；
Step 2) test card 10 enter lighting unit 5 operating area, microprocessor 4 control 5 pairs of examinations of lighting unit
Paper card 10 exports illumination light, and controls 6 pairs of test cards 10 of the shooting unit to carry out optical pickup, draws shooting result；
Step 3) control of microprocessor 4 graphics processing unit 7 pairs of steps 2) shooting result be analyzed treatment,
When analysis processing result draws the detection line gray value of test card 10 and nature controlling line gray value, nature controlling line gray scale is calculated
Value draws testing result with the ratio of detection line gray value, carries out step 7)；
When analysis processing result draws the nature controlling line gray value of test card 10, and when not drawing detection line gray value, detection knot
Fruit is 0, carries out step 7)；
When analysis processing result draws the detection line gray value of test card 10, and when not drawing nature controlling line gray value, detection knot
Fruit is invalid, carries out step 7)；
When analysis processing result draws be not detected by test card 10 nature controlling line gray value and detection line gray value, carry out
Step 4)；
Step 4) microprocessor 4 control 8 pairs of test cards 10 of laser carry out laser irradiation, send out the test card 10
Go out fluorescence；
Step 5) the first photoelectric sensor 9 receives the step 4) in fluorescence and be processed as electric signal and send to described micro-
Processor 4；
Step 6) microprocessor 4 processes the step 5) described in electric signal draw fluorescent bands result；
When fluorescent bands result draws the nature controlling line fluorescence intensity level of test card 10 and detection line fluorescence intensity level, matter is calculated
Control line fluorescence intensity level draws testing result with the ratio of detection line fluorescence intensity level；
When fluorescent bands result draws the nature controlling line fluorescence intensity level of test card 10, and detection line fluorescence intensity level is not drawn
When, testing result is 0；
When fluorescent bands result draws the detection line fluorescence intensity level of test card 10, and nature controlling line fluorescence intensity level is not drawn
When, testing result is invalid；
Step 7) testing result feeds back to display screen 3 and shown by microprocessor 4；
Step 8) return to step 2) carry out next test card 10 and detect.
It should be pointed out that body of the invention 1 is provided with corresponding controlling switch button.
When using, the arrangement of test card 10 that will scribble sample is positioned in the draw-in groove 13 of test paper disk 11, and by test card 10
Made marks with corresponding numbering 19, be easy to record experimental result；After test card 10 is placed on test paper disk 11, test paper disk 11 is consolidated
Due on sample injection disc 12, and cause that alignment pin 21 coordinates with positioning through hole 20.When needing sample introduction, by being opened accordingly on body 1
Button is closed, the work of 4 controlled motor of microprocessor 16 is rotated so as to drive gear 15, and the tooth bar 14 coordinated with it i.e. sample injection disc 12 enters
Sample, due to the effect of the second photoelectric sensor 17 and catch 18, sample injection disc 12 proceeds to relevant position；The control of microprocessor 4 is shone
Bright unit 5 exports illumination light to first test card 10 on test paper disk 11, and controls the shooting unit 6 pairs test card 10 to carry out
Optical pickup, draws shooting result；Microprocessor 4 controls graphics processing unit 7 that treatment is analyzed to shooting result again, draws
The nature controlling line gray value of test card 10 and detection line gray value；Microprocessor 4 is by calculating nature controlling line gray value and detection line gray scale
The ratio of value draws testing result, and testing result is fed back into display screen 3 is shown, microprocessor 4 controls electricity again afterwards
Machine 16 works, under the position-limiting action of the second photoelectric sensor 17 and catch 18, sample injection disc 12 move forward two test cards 10 away from
From so that second test card 10 on paper disc is in the illumination zone of lighting unit 5, is carried out successively.
If above-mentioned microprocessor 4 cannot draw first by calculating nature controlling line gray value with the ratio of detection line gray value
The testing result of test card 10, then deducibility first test card 10 is dead card or immunofluorescence test card, then microprocessor
The control laser 8 of device 4 carries out laser irradiation to test card 10, test card 10 is sent fluorescence；First photoelectric sensor 9 is received should
Fluorescence is simultaneously processed as electric signal and sends to microprocessor 4；Microprocessor 4 processes the electric signal and draws testing result, and will detection
Result feeds back to display screen 3 and is shown, carries out next test card 10 and detects；Carry out successively, until the examination on test paper disk 11
All detection is finished paper card 10, and the controlled motor 16 of microprocessor 4 works, and sample injection disc 12 is exited from turnover sample opening 2.
Claims (8)
1. a kind of collaurum quantitative fluorescence analysis all-in-one, it is characterised in that：Including body (1) and on the body (1)
Turnover sample opening (2) and display screen (3)；Microprocessor (4) is provided with the body (1) and is connected with the microprocessor (4)
Lighting unit (5), shooting unit (6), graphics processing unit (7), laser (8) and the first photoelectric sensor (9) for connecing；
Turnover sample opening (2) is for being put into or taking out test card (10)；
The lighting unit (5) to the test card (10) for exporting illumination light；
The shooting unit (6) to the test card (10) for carrying out optical pickup and draws shooting result；
Described image processing unit (7) is analyzed treatment for the shooting result to the shooting unit (6), draws test card
(10) nature controlling line gray value and detection line gray value；
The laser (8) makes the test card (10) send fluorescence for carrying out laser irradiation to the test card (10)；
First photoelectric sensor (9) is sent to described for receiving the fluorescence and being processed as electric signal according to fluorescence intensity
Microprocessor (4)；
The microprocessor (4) is by calculating the ratio of nature controlling line gray value and detection line gray value or processing first photoelectricity
The electric signal of sensor (9) draws testing result；
The display screen (3) to the testing result for showing.
2. collaurum quantitative fluorescence analysis all-in-one according to claim 1, it is characterised in that：In the body (1) also
It is provided with the sampling device for being connected with the microprocessor (4) and the body (1) being stretched out by turnover sample opening (2)
(22)；Test paper disk (11) of the sampling device (22) including the level for holding multiple test cards (10) and for solid
The sample injection disc (12) of the level of the fixed test paper disk (11)；Test paper disk (11) upper surface is provided with multiple with the test card
(10) draw-in groove (13) of corresponding matching；Sample injection disc (12) bottom is provided with slide rail, and sample injection disc (12) side wall is provided with along side wall side
To the tooth bar (14) for setting；The gear (15) and the driving tooth coordinated with the tooth bar (14) are additionally provided with the body (1)
The motor (16) that wheel (15) is rotated；The motor (16) is connected with the microprocessor (4)；The lighting unit (5), shooting are single
First (6), laser (8) and the first photoelectric sensor (9) are directly over the test paper disk (11).
3. collaurum quantitative fluorescence analysis all-in-one according to claim 2, it is characterised in that：In the body (1) also
The second photoelectric sensor (17) located at the sampling device (22) both sides is provided with, the test paper disk (11) is in each draw-in groove
(13) side is provided with the raised catch (18) for cutting off the second photoelectric sensor (17) signal, second photoelectric sensing
Device (17) is connected with the microprocessor (4).
4. the collaurum quantitative fluorescence analysis all-in-one according to Claims 2 or 3, it is characterised in that：Also include being sticked
On the test paper disk (11) or on the test card (10) for the test card (10) to be divided into colloidal gold test paper card
Or the identification code of immunofluorescence test card.
5. the collaurum quantitative fluorescence analysis all-in-one according to Claims 2 or 3, it is characterised in that：The test paper disk
(11) with the sample injection disc (12) removable connection.
6. the collaurum quantitative fluorescence analysis all-in-one according to Claims 2 or 3, it is characterised in that：The test paper disk
(11) it is provided with positioning through hole (20)；The sample injection disc (12) is provided with the alignment pin coordinated with the positioning through hole (20)
(21)。
7. the collaurum quantitative fluorescence analysis all-in-one according to claim 1 or 2 or 3, it is characterised in that：The test card
(10) it is colloidal gold test paper card or immunofluorescence test card.
8. a kind of control method of collaurum quantitative fluorescence analysis all-in-one as claimed in claim 1, it is characterised in that：Including
Following steps：
Step 1) test card (10) is put into body (1) by passing in and out sample opening (2)；
Step 2) test card (10) enter lighting unit (5) operating area, microprocessor (4) control lighting unit (5) to institute
Test card (10) output illumination light is stated, and controls the shooting unit (6) to carry out optical pickup to the test card (10), drawn
Shooting result；
Step 3) microprocessor (4) control graphics processing unit (7) to the step 2) shooting result be analyzed treatment,
When analysis processing result draws test card (10) detection line gray value and/or nature controlling line gray value, according to nature controlling line ash
The ratio of both angle value, detection line gray value or calculating draws testing result, carries out step 7)；
When analysis processing result draws is not detected by nature controlling line gray value and detection line gray value on test card (10), walked
It is rapid 4)；
Step 4) microprocessor (4) control laser (8) laser irradiation is carried out to the test card (10), make the test card
(10) fluorescence is sent；
Step 5) the first photoelectric sensor (9) receives the step 4) in fluorescence and be processed as electric signal and send to micro- place
Reason device (4)；
Step 6) microprocessor (4) the treatment step 5) described in electric signal draw fluorescent bands result；
When fluorescent bands result draws test card (10) nature controlling line fluorescence intensity level and detection line fluorescence intensity level, according to Quality Control
The ratio of line fluorescence intensity and both detection line fluorescence intensity level or calculating draws testing result；
Step 7) testing result feeds back to display screen (3) and shown by microprocessor (4)；
Step 8) return to step 2) carry out next test card (10) detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710046763.3A CN106855515B (en) | 2017-01-22 | 2017-01-22 | Colloid Jin Yingguang quantitative analysis integrated machine and control method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710046763.3A CN106855515B (en) | 2017-01-22 | 2017-01-22 | Colloid Jin Yingguang quantitative analysis integrated machine and control method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106855515A true CN106855515A (en) | 2017-06-16 |
| CN106855515B CN106855515B (en) | 2023-11-24 |
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ID=59125244
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710046763.3A Active CN106855515B (en) | 2017-01-22 | 2017-01-22 | Colloid Jin Yingguang quantitative analysis integrated machine and control method thereof |
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| Country | Link |
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| CN (1) | CN106855515B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108303423A (en) * | 2018-02-12 | 2018-07-20 | 广州眺望电子科技有限公司 | A kind of simple efficiently fluorescin reagent card digital independent device and method |
| CN109738622A (en) * | 2018-12-29 | 2019-05-10 | 北京化工大学 | Lateral flow paper slip device for fast detecting based on micro-fluidic chip |
| CN110531097A (en) * | 2019-10-11 | 2019-12-03 | 北京中航赛维生物科技有限公司 | A kind of colloidal gold fluorescence one immunity analysis instrument |
| CN111795959A (en) * | 2020-07-24 | 2020-10-20 | 芯动森林(重庆)医疗科技有限公司 | Colloidal gold and fluorescence two-in-one data acquisition device and method |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101275898A (en) * | 2007-03-29 | 2008-10-01 | 希森美康株式会社 | Blood sample analyzer |
| JP2009041953A (en) * | 2007-08-06 | 2009-02-26 | Yaskawa Information Systems Co Ltd | Quantitative analyzer, quantitative analyzing method and quantitative analyzing program |
| US20110076695A1 (en) * | 2008-05-29 | 2011-03-31 | Arkray, Inc. | Immunoassay analyzer and immunoassay method |
| WO2011154918A2 (en) * | 2010-06-09 | 2011-12-15 | Medisensor Gh, Inc. | Diagnostic apparatus for immunoassay and diagnostic method for immunoassay using the same |
| CN204008657U (en) * | 2013-10-31 | 2014-12-10 | 闽浪仪器科技（厦门）有限公司 | A kind of based on multispectral fluorescence immune chromatography quantified system analysis |
| CN204255956U (en) * | 2014-10-29 | 2015-04-08 | 杭州中翰盛泰生物技术有限公司 | A kind of full-automatic real-time test instrument pick-up unit |
| US20150212074A1 (en) * | 2012-07-24 | 2015-07-30 | Fio Corporation | Immunoassay rapid diagnostic test universal analysis device, system, method and computer readable medium |
| CN104914239A (en) * | 2015-06-29 | 2015-09-16 | 上海交通大学 | Immunochromatographic test strip detection equipment |
| US20150268239A1 (en) * | 2012-11-28 | 2015-09-24 | Furukawa Electric Co., Ltd. | Immunochromatography, and detection device and reagent for the same |
| KR20150110902A (en) * | 2014-03-20 | 2015-10-05 | 주식회사 메카시스 | An optical sample analyzer for color and fluorescence test strips, a method for image-taking and quantifying measured data employing the same. |
| CN105259342A (en) * | 2013-06-08 | 2016-01-20 | 张沈平 | Testing method for colloidal gold card reader and colloidal gold card reader |
| CN105259171A (en) * | 2015-11-11 | 2016-01-20 | 天津九安医疗电子股份有限公司 | Automatic immersion system and portable urine analyzer |
| CN105784986A (en) * | 2016-04-29 | 2016-07-20 | 江苏金标世纪生物科技有限公司 | Clamping strip type full-automatic sample loading detection immunity analyzer |
| CN206450598U (en) * | 2017-01-22 | 2017-08-29 | 武汉璟泓万方堂医药科技股份有限公司 | Collaurum quantitative fluorescence analysis all-in-one |
-
2017
- 2017-01-22 CN CN201710046763.3A patent/CN106855515B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101275898A (en) * | 2007-03-29 | 2008-10-01 | 希森美康株式会社 | Blood sample analyzer |
| JP2009041953A (en) * | 2007-08-06 | 2009-02-26 | Yaskawa Information Systems Co Ltd | Quantitative analyzer, quantitative analyzing method and quantitative analyzing program |
| US20110076695A1 (en) * | 2008-05-29 | 2011-03-31 | Arkray, Inc. | Immunoassay analyzer and immunoassay method |
| WO2011154918A2 (en) * | 2010-06-09 | 2011-12-15 | Medisensor Gh, Inc. | Diagnostic apparatus for immunoassay and diagnostic method for immunoassay using the same |
| US20150212074A1 (en) * | 2012-07-24 | 2015-07-30 | Fio Corporation | Immunoassay rapid diagnostic test universal analysis device, system, method and computer readable medium |
| US20150268239A1 (en) * | 2012-11-28 | 2015-09-24 | Furukawa Electric Co., Ltd. | Immunochromatography, and detection device and reagent for the same |
| CN105259342A (en) * | 2013-06-08 | 2016-01-20 | 张沈平 | Testing method for colloidal gold card reader and colloidal gold card reader |
| CN204008657U (en) * | 2013-10-31 | 2014-12-10 | 闽浪仪器科技（厦门）有限公司 | A kind of based on multispectral fluorescence immune chromatography quantified system analysis |
| KR20150110902A (en) * | 2014-03-20 | 2015-10-05 | 주식회사 메카시스 | An optical sample analyzer for color and fluorescence test strips, a method for image-taking and quantifying measured data employing the same. |
| CN204255956U (en) * | 2014-10-29 | 2015-04-08 | 杭州中翰盛泰生物技术有限公司 | A kind of full-automatic real-time test instrument pick-up unit |
| CN104914239A (en) * | 2015-06-29 | 2015-09-16 | 上海交通大学 | Immunochromatographic test strip detection equipment |
| CN105259171A (en) * | 2015-11-11 | 2016-01-20 | 天津九安医疗电子股份有限公司 | Automatic immersion system and portable urine analyzer |
| CN105784986A (en) * | 2016-04-29 | 2016-07-20 | 江苏金标世纪生物科技有限公司 | Clamping strip type full-automatic sample loading detection immunity analyzer |
| CN206450598U (en) * | 2017-01-22 | 2017-08-29 | 武汉璟泓万方堂医药科技股份有限公司 | Collaurum quantitative fluorescence analysis all-in-one |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108303423A (en) * | 2018-02-12 | 2018-07-20 | 广州眺望电子科技有限公司 | A kind of simple efficiently fluorescin reagent card digital independent device and method |
| CN109738622A (en) * | 2018-12-29 | 2019-05-10 | 北京化工大学 | Lateral flow paper slip device for fast detecting based on micro-fluidic chip |
| CN109738622B (en) * | 2018-12-29 | 2020-12-11 | 北京化工大学 | Lateral flow paper strip rapid detection device based on micro-fluidic chip |
| CN110531097A (en) * | 2019-10-11 | 2019-12-03 | 北京中航赛维生物科技有限公司 | A kind of colloidal gold fluorescence one immunity analysis instrument |
| CN111795959A (en) * | 2020-07-24 | 2020-10-20 | 芯动森林(重庆)医疗科技有限公司 | Colloidal gold and fluorescence two-in-one data acquisition device and method |
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