KR20150110902A - An optical sample analyzer for color and fluorescence test strips, a method for image-taking and quantifying measured data employing the same. - Google Patents

Abstract

The present invention relates to an optical sample analyzer. More specifically, the optical sample analyzer calculates concentration of a sample reactant. The present invention comprises: a measurement strip module (10) having a measurement strip and a measurement strip storage unit; a first light source unit (21) emitting light; a second light source unit (22) emitting the light; a first collimator unit (31) transmitting the light irradiated from the first light source unit (21); a second collimator unit (32) transmitting the light irradiated from the second light source unit (22); an optical module receiving the light from an irradiation region (11); and an image detection unit (50) having a sensitivity control function. The optical sample analyzer for color and fluorescence measuring strips, wherein optical alignment of the irradiation region (11) and the optical module (40) and the image detection unit (50) is performed, is provided.

Description

TECHNICAL FIELD The present invention relates to an optical sample analyzer for color and fluorescence measurement strips, an image capturing method using the same, and an optical sample analyzer for color and fluorescence test strips, a method for image-taking and quantifying measured data employing the same.

The present invention relates to an optical sample analyzer, and more particularly, to an optical sample analyzer that irradiates light having a predetermined wavelength to a measurement object, captures an image to be generated, and analyzes the concentration of the reactant.

In recent years, the development of diagnostic kits for identifying SARS (severe acute respiratory syndrome) and poultry influenza virus (avian influenza) have been successfully developed. Without using expensive medical equipment, one or two drops of blood or urine can be used accurately and easily The development of diagnostic kits is being actively pursued. In addition to widely used pregnancy diagnostic kits, diagnostic kits for diagnosing various diseases ranging from infectious diseases such as hepatitis, tuberculosis and AIDS to various cancers, diabetes and heart diseases have also been developed and marketed.

When a person is suffering from a certain disease, a biomarker is used in which the antibody, hormone, or protein in the body fluid such as blood is markedly high or low. In the case of the diagnostic kit, a reagent-treated measurement strip is used for analysis The diagnosis uses the color change of the reagent that occurs during the reaction between the biomarker contained in the sample and the reagent.

As an optical sample analyzer, a color analyzer for reading reflected light generated by irradiating light of a visible light region to a sample surface and analyzing the concentration of a corresponding component in the sample reactant, a sample or a reagent is labeled with a specific fluorescent substance, There is a fluorescence analyzer which irradiates light having an exciting wavelength inherent to the fluorescent substance, reads out the light of the emitted fluorescence, and detects the presence or absence of the substance and analyzes the concentration.

In Korean Patent No. 10-0737395 entitled "Automatic Color Measurement System for Clinical Chemical Analysis and Method Thereof, hereinafter referred to as Prior Art 1"), input means of functions such as various mode selection, A display unit for displaying an inputted command or setting state, a sensor driving means for moving the color sensor, a position sensor for detecting an initial position of the color sensor, setting information, A main controller for controlling the operation of the means and the color sensor, temporarily storing the color sensor measurement information, processing and outputting the color sensor measurement information, and a computer for receiving and analyzing the measurement data and displaying the analyzed data in various forms An automatic color measurement system for a color image is disclosed.

KR 10-0737395 B1

Prior Art 1 is a system that utilizes only light in the visible light region, and a separate system is required to have a fluorescence analysis function for measuring the concentration of a fluorescently labeled reactant.

Further, in operating the color sensor, there is a complication that it is necessary to move the sensor using the sensor driver every time of focusing to photograph the image of the sample, and furthermore, it is judged whether or not the focusing element And the like.

The present invention has been proposed in response to the above-mentioned problems and requirements, and includes a measurement strip module 10 including a measurement strip having a radiation area 11 and a measurement strip compartment 10, A first light source section 21 for emitting light to be irradiated to the region 11, a second light source section 21 having a wavelength of a predetermined region capable of exciting the fluorescent substance in the sample reactant, A first collimator unit 31 having a function of transmitting the light irradiated from the first light source unit 21 and the second light source unit 22 and converting the light to be irradiated with a uniform illuminance throughout the irradiation area 11, A second collimator unit 32 having a function of transmitting light radiated from the second light source unit 22 and converting the light into irradiated light with a uniform illumination over the irradiation area 11; And only a predetermined wavelength band is selected And a function of condensing light, a function of photographing a two-dimensional image of light transmitted from the optical module 40 and converting the two-dimensional image into an electric signal, and a function of adjusting a sensitivity Dimensional image of the irradiation area 11 is focused on the image detector 50. The two-dimensional image of the irradiation area 11 is focused on the image detector 50, the optical sample analyzer for both color and fluorescence measurement strips is provided.

The present invention also provides a method for photographing reflected light or emitted light image for each measurement mode using an optical sample analyzer, and a method for quantifying the concentration of the sample reactant by processing the collected two-dimensional image.

The optical sample analyzer of the present invention has both a color analysis function using light in the visible light region and a fluorescence analysis function using light having excitation wavelength so that the two modes can be individually selected or simultaneously selected for measurement and analysis Therefore, the time and cost of the test can be reduced. In addition, by using the image sensor 51 capable of adjusting the sensitivity, it is possible to reduce the need to replace the image sensor 51 for each measurement mode.

Further, workability is improved by ensuring image focusing through the physical alignment of the optical module 40, the irradiation area 11 on the measurement strip module 10, and the image detection part 50, which are components.

It also provides an algorithm for determining the concentration of the sample reactant from the two-dimensional image of the measured irradiance area 11 to ensure automated analysis work by the computer.

1 is a perspective view showing a configuration of an embodiment of the optical sample analyzer of the present invention.
2 is a perspective view showing a configuration of an embodiment of the optical sample analyzer of the present invention.
3 is an explanatory view showing the function of one embodiment of the optical sample analyzer of the present invention.
4 is a graph illustrating a principle of selecting a reference wavelength of a cutoff filter of an optical module 40 in an embodiment of the present invention.
5 is a flowchart showing a method of photographing a two-dimensional image of the irradiation area 11 on the measurement strip using the optical sample analyzer of the present invention.
FIG. 6 is an explanatory diagram showing an embodiment for setting a two-dimensional image of the irradiation area 11 measured by using the optical sample analyzer of the present invention and a limited area for data processing.
7 is a flowchart showing a method of quantifying the concentration of the sample reactant in the signal processing unit 70 of the present invention.
8 is an explanatory view showing an embodiment for determining the first position 81, the second position 82, and the third position 83, which are parameters used in the method of quantifying the concentration of the sample reactant of the present invention .

The optical sample analyzer for both color and fluorescence measurement strips of the present invention is responsible for irradiating the measurement strip with incident light, visible light or ultraviolet light, and taking a two-dimensional image of the resulting irradiation area. Further, it may further include a part for processing the photographed two-dimensional image to quantify the concentration value of the actual sample reactant, which may be implemented by software or by a separate device.

The spectrometer of the present invention can support the following three measurement modes. The first mode is a color measurement mode in which light in the visible light region is irradiated to the irradiation region 11 and the generated image is detected and used. In the second mode, the fluorescent substance contained in the sample reactant is excited And a fluorescence measurement mode in which the emitted light is sensed and used. The third mode is a combined color and fluorescence measurement mode that simultaneously performs fluorescence measurement for high-concentration reactants and color measurement for low-concentration reactants. Of course, in order to be able to measure the third mode, it is necessary to assume that the emission wavelength of the fluorescent material and the band of the wavelength of the color-reflected light are close to each other. FIG. 3 shows a schematic principle of using the optical sample analyzer of the present invention in the third mode. The light emitted from the first light source unit 21 and the second light source unit 22 passes through the first collimator unit 31 and the second collimator unit 32 and is irradiated onto the irradiation area 11 of the measurement strip. The emitted light and the reflected light formed are photographed by the image detector 50 via the condenser 41 and the wavelength selector 42. [ The present invention has the advantage of simultaneously performing fluorescence analysis and visible light analysis.

Referring to one embodiment of the present invention shown in FIG. 1, the function and structure of each component of the present invention will be described below. The measurement strip module 10 comprises a measurement strip and a measurement strip compartment for receiving the measurement strip. The measuring strip is reagent treated and is made of paper or other material capable of absorbing and containing the reagent, the sample and its reactant, which is subjected to a predetermined chemical reaction when the sample is dispensed or applied thereon. The measurement strip accommodating portion is made of a material that does not react with the sample material. The measurement strip is first put in a container, and then the sample material is dispensed or applied to the measurement strip to allow the sample to react with the sample. The measurement strip may be accommodated. The measuring strip housing part is provided with an element for allowing the measuring strip to be mounted and fixed at a predetermined position inside the measuring strip housing part so that when the measuring strip housing part is positively positioned in relation to the incident light, So that incident light can be accurately irradiated to the irradiation region.

The first light source part 21 and the second light source part 22 function to emit light having a wavelength of a predetermined area and can be constituted by a point light source or a surface light source. In the case of a point light source, a reflector may be provided around the point light source to concentrate the light radiated to the irradiation area 11 on the measurement strip. For the first mode and the third mode of the measurement mode of the present invention, the first light source unit 21 may emit white visible light. The white visible light is a mixture of the wavelengths of the seven colors of light in the visible light region. When the light is irradiated on the surface of the measurement strip - white strip - before the reaction, theoretically, White light. However, since the actual white strip is not completely white, it is necessary to correct the color, which is related to white balancing, which will be described later. When the sample and the reagent are determined, the color of the sample reactant formed by the reaction is determined. When white light is irradiated to the reaction site where the sample reactant on the measuring strip is formed, light of a wavelength unique to the sample reactant is absorbed The light of the remaining wavelength is reflected, and the reflected light is taken as an object of analysis. The first light source unit 21 may be a white LED module for emitting white light, but a halogen lamp, a Xe arc lamp, or the like may be used.

For the second mode and the third mode of the measuring mode of the present invention, the second light source unit 22 may emit light of a wavelength capable of exciting the fluorescent substance contained in the sample reactant. In general, when a fluorescent material is irradiated with light having an exciting wavelength of a predetermined region, the energy level is excited and becomes higher, and again, when the energy level is lowered, light having a predetermined emission wavelength The light that is observed during this process is fluorescent light, and its wavelength is longer than the exciting wavelength. The second light source unit 22 may be formed of a mercury lamp, but it is preferable that the second light source unit 22 is composed of an LED module or a laser diode (LD) in view of output performance and life span. Of course, even in the second mode, the second light source unit 22 need not be limited to the light in the ultraviolet region, if the fluorescent material concerned can be excited by receiving visible light.

In the present invention in which incident light is irradiated to the irradiation region and the intensity of the incident light differs from site to region of the measuring strip, accurate measurement values can not be obtained. Therefore, incident light is irradiated uniformly over the irradiation region 11 . The first collimator unit 31 and the second collimator unit 32 are disposed so as to irradiate the light irradiated from the first light source unit 21 and the second light source unit 22 on the irradiation area 11 of the measurement strip, And converts them into parallel light, which converts the illuminated light into parallel light. Particularly, when the first light source unit 21 and the second light source unit 22 are point light sources, the first collimator unit 31 and the second collimator unit 32 may have a shape of a convex lens, It may also consist of a diffuser. In the embodiment of the present invention, the first collimator unit 31 and the second collimator unit 32 are disposed such that the paths of the light beams passing through the first collimator unit 31 and the second collimator unit 32 are v-shaped, And the second collimator can be directly irradiated onto the irradiation area 11 of the measurement strip and the optical module 40 for collecting the two-dimensional image of the irradiation area can be irradiated to the vertical It is possible to secure a space that can be disposed above. Of course, the first collimator unit 31 and the second collimator unit 32 are disposed such that the light transmitted through the first collimator unit 31 and the second collimator unit 32 is parallel to the irradiation area 11 plane However, in this case, an additional component such as a prism is required to redirect the transmitted light to the irradiation area 11 of the measurement strip.

The optical module 40 focuses the color-reflected light (the first mode and the third mode) or the fluorescence emission light (the second mode and the third mode) from the irradiation area 11 and at the same time, And has a function of transmitting. Since the irradiation area 11 on the measurement strip does not function as a mirror, the irradiated light is reflected in all directions. Therefore, in order to detect an image, it is necessary to gather and collect light reflected from all directions. Since the first light source unit 21 and the second light source unit 22 are located adjacently to the optical module 40, the size of the optical module 40 is smaller than that of the first light source unit 21 and the second light source unit 22, It should be limited in size so as not to interfere with the path of light emitted from the light source.

In particular, in relation to the second mode and the third mode, since the reflected or emitted light to be analyzed by the present invention is limited to the emission wavelength of the fluorescent material and the color-reflected wavelength of light, the signal relating to the excitation wavelength of the fluorescent material is collected Should be excluded. This is because the excitation wavelength is shorter than the emission wavelength of the fluorescent material or the wavelength of the color-reflected light, so that the image detector 50 having sensitivity set to detect the emission wavelength of the fluorescent material or the color- It is also possible to generate a large noise. Various filter functions may be introduced to selectively transmit only light having a wavelength greater than a predetermined wavelength value in order to select and process only a part of the light from the reflected light or emitted light, Lt; / RTI > should be a value existing between the excitation wavelength value and the emission wavelength value. In this case, the filter selectively transmits color-reflected light and emitted light of the fluorescent material.

The optical module 40 may be constructed so as to concentrate the condensing function and the wavelength selecting function described above into one element. However, the optical module 40 may be constructed separately from the condensing section 41 having the condensing function and the wavelength selecting section 42 having the wavelength selecting function . In the embodiment of the present invention, the light-collecting portion 41 is formed of a convex lens, and the wavelength selector 42 blocks the excitation wavelength (short-wavelength) light of the fluorescent material and emits light of the emission wavelength (long wavelength) In order to transmit only color-reflected light in a visible light region having a long wavelength, a cut-off filter which transmits only light having a wavelength of a predetermined wavelength or more is adopted. FIG. 4 shows a criterion for selecting the reference wavelength of such a cut-off filter. At this time, in the relative layout of the wavelength selecting section 42 and the light condensing section 41, the reflected light or the emitted light may be transmitted first through the wavelength selecting section 42 or may be transmitted through the light collecting section 41 first It is possible.

The image of the irradiation area 11 transmitted through the wavelength selection part 42 and the light condensing part 41 of the optical module 40 is photographed by the image detection part 50 as a two dimensional image and converted into an electric signal and used . The image detector 50 may have a sensitivity control function because wavelengths of light to be measured are different in the first mode, the second mode and the third mode. For example, in order to measure the first mode, emission light of the fluorescent substance to be measured in the second mode is photographed using the image detecting unit 50 set for the visible light region, When the color-reflected light, which is the measurement object of the first mode, is photographed using the set image detector 50, the required resolution can not be obtained. Therefore, prior to the photographing step, the sensitivity of the image detector 50 A step of adjusting the temperature should be preceded.

The image detecting unit 50 may include any one of an image sensor 51 (CCD), a photodiode array, and an image sensor 51. In the image sensor 51 (CCD, Charge Coupled Device), a plurality of pixels each having a photosensor connected to a condenser are arranged, and when the light is incident, the photosensor converts the light into electric charge, charges the capacitor, . The photodiode array is a one-dimensional or two-dimensional array of photodiode chips for converting detected optical signals into electrical signals. The photodiode array uses the fact that electrical signals vary in size in proportion to the size of optical signals.

The irradiation area 11, the optical module 40 and the image detection part 50 should be optically aligned on a single axis so that the two-dimensional image of the irradiation area 11 is focused on the image detection part 50, , It is possible to omit a separate image focusing operation for each image taking. In the embodiment, the image sensor 51 is mounted on the image sensor holder 52 and the image sensor 51 is aligned on the optical axis by inserting the image sensor 51 into the image sensor insertion hole 43 formed in the optical module 40. Alternatively, the image sensor holder 52 and the optical module 40 may have separate alignment portions for aligning the optical axes, respectively, and the image sensor 51 and the light collecting portion 41) (lens) and the irradiation area 11 can be assured. For example, when the image sensor holder 52 is seated with a small tolerance in the groove formed on the upper surface of the optical module 40, the image sensor 51 mounted on the image sensor holder 52 and the optical module 40 ), Or a method of forming an alignment groove in the image sensor holder 52 and an alignment groove portion in the optical module 40, and combining the optical axis and the lens.

2, it is possible to further include the casing unit 60 so that the above-described components - the measuring strip module 10, the first light source unit 21, the second light source unit 22 (see FIG. 2) If the first collimator unit 31, the second collimator unit 32, the optical module 40, and the image detecting unit 50 are accommodated therein, the above-mentioned alignment of the optical axis can be prevented from being destroyed by an external force In addition, in the process of photographing the two-dimensional image of the irradiation area 11, the casing unit 60 forms a kind of dark room, so that it is possible to block external light noise and obtain a more accurate image .

The method of photographing the two-dimensional image of the irradiation area 11 for measuring the concentration of the sample reactant using the spectroscopic measurement apparatus of the present invention can be performed by the following procedure with reference to FIG. First, a measurement mode is selected from a color measurement mode, a fluorescence measurement mode, a color and fluorescence composite measurement mode, and a measurement strip module 10 is installed. Second, the sensitivity of the image detector 50 is adjusted to the previously selected measurement mode. Third, in the color measurement mode, the first light source unit 21 is driven. In the fluorescence measurement mode, the second light source unit 22 is driven. In the color and fluorescence composite measurement mode, The first light source unit 21 and the second light source unit 22 are simultaneously driven. Fourthly, a two-dimensional image of the irradiation area 11 is photographed by using the image detecting part 50.

The signal processing unit 70 may further include a signal processing unit 70 for receiving and analyzing the two-dimensional image captured by the image detecting unit 50 to quantify and derive the concentration of the sample reactant. The signal processing unit 70 may include an input unit 71, a memory unit 73, a central processing unit 72 and a mapping data file. When a final measurement value is given to the mapping data file, You can record a table to find the corresponding sample reactant concentration value.

Referring to the flowchart shown in FIG. 7, the procedure of the information processing in the signal processing unit 70 will be described as follows. Determining the final measurement value and determining the concentration of the sample reactant is applied to both the color measurement mode, the fluorescence measurement mode, and the color and fluorescence composite measurement modes. First, the two-dimensional image of the irradiation area 11 photographed by the image detecting unit 50 is stored in the memory unit 73 through the input unit 71 of the signal processing unit 70. [ Second, the central processing unit 72 defines a predetermined area in the two-dimensional image, and determines an image attribute value corresponding to each horizontal axis value in a predetermined area, and stores the determined image attribute value in the memory unit 73. In an embodiment in which a plurality of band-shaped reaction parts are formed on the measurement strip with the same width in the longitudinal direction, the predetermined area may be set to have a rectangular shape, And the longitudinal length may be set so as to have a certain ratio (for example, 1 / 2-) to the entire longitudinal length of the measurement strip. In the embodiment shown in FIG. 6, the predetermined area has a red rectangular shape and defines a part of the two-dimensional image of the irradiation area 11. The image attribute value may be a color value and a brightness value. If a reagent and a sample for a specific reaction are determined in advance, the color value of the band-shaped reaction part is automatically determined, Only the brightness value is displayed. Third, the central processing unit 72 determines the horizontal axis first position 81 having the smallest image attribute value stored in the memory. At this time, when a plurality of band-shaped reaction portions are formed, the first position 81 should be determined for each reaction portion, and the number of the first positions 81 determined is equal to the number of the band-shaped reaction portions. Fourth, when the central processing unit 72 is moved from the first position 81 to the second position 82 spaced apart by a predetermined distance and from the first position 81 to the third position 83 separated from the first position 81 by a predetermined distance From the image attribute values stored in the memory unit 73. [0050] Fifth, the central processing unit 72 calculates an image attribute value at the second position 82 and an average value of the image attribute value at the third position 83. Sixth, the central processing unit 72 calculates the difference between the image attribute value and the average value at the first position 81 and derives the final measurement value. Since the average value of the image attribute values at the positions spaced left and right by a predetermined distance from the first position 81 is subtracted from the second position 82 and the third position 82, It is preferable that the electrode 83 is not located inside the band-shaped reaction part, and therefore, the predetermined distance may be set as the length of the width of the band-shaped reaction part. In the embodiment shown in FIG. 8, the image attribute values corresponding to the horizontal axis values of the respective points are shown in a two-dimensional graph. Two reaction sections are formed, and the first position 81, The second position 82 and the third position 83 are determined. The distance between the first position 81 and the second position 82 and the distance between the first position 81 and the third position 83 are the same, Is set. Seventh, the central processing unit 72 extracts the sample reactant concentration corresponding to the final measured value from the mapping data file. Generally, the darker the band-shaped reaction part, the greater the concentration of the sample reactant. If the mapping data file quantitatively mapping the correlation is prepared in advance through experiments, the concentration of the sample reactant corresponding to the final measurement value Can be determined by referring to the mapping data file.

Under the assumption that the first collimator unit 31 and the second collimator unit 32 form light having a uniform illuminance with respect to the irradiation area 11, when the white collimator unit 31 and the second collimator unit 32 are measured with a white strip, The image attribute value-brightness value at all points will be measured equally. However, if such assumptions do not hold, additional steps of white balancing are needed to ensure unequivocal validity-uniformity of the image attribute values computed at each point. In this method, before the measurement on the measurement strip is performed, the image attribute values measured by mounting the white strip are stored in the memory unit 73, and then the local image attribute values are obtained for the measurement strip In the process, the previously stored white strip image attribute value is subtracted and stored.

Such a method can be executed in a general-purpose digital computer that can be created as a program that can be executed by a computer and operates the program using a computer-readable recording medium. The computer-readable recording medium includes a magnetic storage medium (ROM, floppy disk, hard disk, etc.), an optical reading medium (such as a CD-ROM or a DVD), and a real time Internet transmission system.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it should be understood that various changes and modifications will be apparent to those skilled in the art. Obviously, the invention is not limited to the embodiments described above. Accordingly, the scope of protection of the present invention should be construed according to the following claims, and all technical ideas which fall within the scope of equivalence by alteration, substitution, substitution and the like within the scope of the present invention, Range. In addition, it should be clarified that some configurations of the drawings are intended to explain the configuration more clearly and are provided in an exaggerated or reduced size than the actual configuration.

Measuring strip module: 10
Survey area: 11
First light source part: 21
Second light source section: 22
The first collimator unit 31
Second collimator part: 32
Optical module: 40
Miners: 41
Wavelength selection part: 42
Image sensor insertion hole: 43
Image detecting unit: 50
Image Sensor: 51
Image Sensor Holder: 52
Casing section: 60
Casing cover: 61
Casing: 62
Signal processing section: 70
Inputs: 71
Central processing unit: 72
Memory part: 73
Mapping data file part: 74
Limited Area: 75
1st position: 81
Second position: 82
Third position: 83

Claims (14)

An optical sample analyzer for irradiating light of a specific wavelength band to a measurement strip and reading the generated light to analyze the concentration of the sample reactant,
A measuring strip module (10) comprising a measuring strip having a radiation area (11) and a measuring strip compartment;
A first light source part (21) having a wavelength of a visible light ray band and emitting light irradiated to the irradiation area (11);
A second light source part (22) having a wavelength of a predetermined region capable of exciting a fluorescent material in the sample reactant and emitting light irradiated to the irradiation area (11);
A first collimator unit 31 having a function of transmitting light irradiated from the first light source unit 21 and converting the light into irradiated light having uniform illumination over the irradiation area 11;
A second collimator unit 32 having a function of transmitting light radiated from the second light source unit 22 and converting the light into illumination light having a uniform illumination over the irradiation area 11;
An optical module (40) having a function of selectively irradiating light of a predetermined wavelength band and a function of collecting light, the light being irradiated with light from the irradiation region (11);
An image detector 50 having a function of photographing a two-dimensional image of light transmitted from the optical module 40 and converting the two-dimensional image into an electrical signal and having a sensitivity adjusting function;
, ≪ / RTI >
The irradiation area 11, the optical module 40 and the image detection part 50 are arranged to perform optical alignment so that the two-dimensional image of the irradiation area 11 is focused on the image detection part 50. [ Wherein the color and fluorescence measurement strips are combined with each other. The method according to claim 1,
The optical module 40 includes a light collecting part 41 for collecting and collecting light from the measurement area and a wavelength selecting part 40 for selectively transmitting only a predetermined wavelength band of light transmitted from the light collecting part 41. [ (42). The optical sample analyzer according to claim 1 or 2, The method according to claim 1,
The first collimator unit 31 and the second collimator unit 32 are arranged in the order of the first collimator unit 31 and the second collimator unit 32, Wherein the optical path of the light passing through each of the plurality of color and fluorescence measurement strips is arranged so as to form a V (v) character. The method of claim 2,
The optical sample analyzer according to claim 1 or 2, wherein the light collecting part (41) has a convex lens shape. The method according to claim 1,
Wherein the first collimator part (31) and the second collimator part (32) are made of a convex lens, a concave lens, or a diffuser (Diffuser). The method according to claim 1,
The first light source unit 21 is a white LED that emits visible light and the second light source unit 22 is an LED or an LED that emits light capable of exciting a fluorescence substance of a sample reactant, Characterized in that it is a laser diode (LD). The method of claim 2,
The wavelength selector 42 is provided to cut off light in an excitation wavelength region for a fluorescent material and to emit light in a wavelength region for emission of a fluorescent material and a light having a cut- -off) filter. The optical sample analyzer according to claim 1,
The method according to claim 1,
The optical sample analyzer according to claim 1, wherein the image detector (50) comprises an image sensor (51) (CCD) or a photodiode array. The method according to claim 1,
The first collimator unit 31, the second collimator unit 32, the optical module 40, and the first and second collimator units 31 and 32 and the first and second light source units 21 and 22, And a casing unit (60) for accommodating the image detecting unit (50) therein. A method of photographing a two-dimensional image of an irradiation area (11) for measuring a concentration of a sample reactant using an optical sample analyzer according to any one of claims 1 to 9,
Selecting (s10) a measurement mode among a color measurement mode, a fluorescence measurement mode and a combined color and fluorescence measurement mode;
Adjusting (20) the sensitivity of the image detector (50) according to the measurement mode;
(S30) driving the first light source unit (21) or the second light source unit (22) according to the measurement mode;
A step (s40) of photographing a two-dimensional image of the irradiation area 11 using the image detecting part 50;
(11) by using an optical sample analyzer for both color and fluorescence measurement strips. The method according to claim 1,
A signal processor 70 for receiving the electrical signal generated by the image detector 50 to quantify and derive a sample reaction concentration; Wherein the signal processing unit 70 comprises an input unit 71, a memory unit 73, a central processing unit 72 and a mapping data file, and the mapping data file includes a final measured value and a corresponding And a table for the sample reactant concentration value is recorded in the optical sample analyzer. A method for quantifying sample reactant concentration using an optical sample analyzer according to claim 11,
A step (s100) in which the two-dimensional image of the irradiation area 11 photographed by the image detecting part 50 is received by the input part 71 and stored in the memory part 73;
(S200) for the central processing unit (72) to define a predetermined area from the two-dimensional image, determine an image attribute value corresponding to each horizontal axis value within the predetermined area, and store the determined value in the memory unit (73);
(S300) the central processing unit (72) determines a horizontal axis first position (81) having the smallest image attribute value stored in the memory unit (73);
The central processing unit 72 includes a second position 82 spaced apart from the first position 81 by a predetermined distance and a third position 83 spaced from the first position 81 by a predetermined distance to the right, (S400) of extracting an image attribute value from the image attribute value stored in the memory unit (73);
(S500) the central processing unit (72) calculating an average value of an image attribute value at the second position (82) and an image attribute value at the third position (83);
Calculating the difference between the image attribute value and the average value at the first position (81) and deriving the difference as the final measured value (s600);
Extracting a sample reactant concentration corresponding to the final measurement value from the mapping data file (s700);
For quantifying the sample reactant concentration using an optical sample analyzer for both color and fluorescence measurement strips The method of claim 12,
The method of claim 1, further comprising the step (s90) of storing a white strip image attribute value in the memory unit (73) before the step (i), wherein the image attribute value in the step (i) Wherein the measured value is a value obtained by white balancing by subtracting the value of the property from the measured value of the sample reactant concentration. A computer-readable recording medium recording a program for executing the method according to claim 12 or 13.

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