CN106854249A - A kind of extraction process of silkworm chrysalis chitin - Google Patents

Abstract

The invention discloses a kind of extraction process of silkworm chrysalis chitin, using residual residue deep processing after silkworm chrysalis processing and eating oil, albumen powder, silkworm chrysalis utilization rate is improve.Its technique includes that silkworm chrysalis clears up removal of impurities, and dries pulverizing, subcritical abstraction degreasing, energy-collecting ultrasonic cycling extraction takes off albumen, decalcification, and sleeping spiral shell is centrifuged to obtain chitin., by the use of silkworm chrysalis shell as raw material chitin extraction, the recovery rate of chitin is high, and content of ashes is low, and nitrogen content is high, takes full advantage of natural resources, and cost is lower for the present invention.The present invention provide not only a kind of technical matters of the large-scale production silkworm chrysalis chitin of simplicity, while also having expanded silkworm chrysalis comprehensive exploitation value.

Description

A kind of extraction process of silkworm chrysalis chitin

Technical field

The invention belongs to insect chitin preparing technical field, and in particular to be a kind of extraction process of silkworm chrysalis chitin.

Background technology

Chitin, also known as chitin, chitin, chitin, glutelin, bright angle glutelin, chitin, crab shell element, insoluble chitin etc., the entitled poly- β-(1 of chemistry, 4) -2- acetamidos -2- deoxidations-D- glucose molecules formula is (C8H15NO6) n, it is the only naturally alkaline polysaccharide for finding so far, element is widely present in the shrimp of Arthropoda Crustacea animal, the crust of crab, it has nontoxic, it is tasteless, it is alkaline-resisting, it is heat-resisting, sun-proof, it is corrosion-resistant, it is resistance to the features such as damage by worms, it is widely used in weaving, printing and dyeing, papermaking, food, medicine, cosmetics, fruit freshness preserving, the fields such as environmental protection.The exploitation about chitin product is obtained from shrimp, crab watchcase at present, because shrimp and crab shell calcareous material are very high, to remove calcium substance, and required reagent consumption is larger.Dry silkworm chrysalis contains chitin about 2.73%, is the very good material for producing chitin, and China is silk big producer, having a large amount of dry silkworm chrysalises every year can utilize, and calcareous material is low in silkworm chrysalis shell, the content of chitin is higher by 5-10% than shrimp crab, during silkworm chrysalis comprehensive utilization is carried out, by pupa albumen, after pupal fat separation and Extraction, remaining residue only needs simple operation just to can obtain chitin, therefore, under equal conditions, cost advantage is had more using silkworm chrysalis shell chitin extraction.

The content of the invention

The present invention is the chitin that the further deep processing of residual residue is food-grade and medical grade after edible oil, albumen powder to solve silkworm chrysalis synthesis deep processing, improves the utilization rate of silkworm chrysalis, there is provided a kind of extraction process of silkworm chrysalis chitin.

What the present invention was realized by following technical scheme：A kind of extraction process of silkworm chrysalis chitin, comprises the following steps：

（1）Silkworm chrysalis is cleared up into removal of impurities, 10~15kg/KWh of microwave drying, pulverizer adds the normal butane of 3-5 times of dried silkworm chrysalis meal quality after crushing, 1.5 in 30~36 DEG C of subcritical abstraction tanks~ 1.8Bar extracts 20-40min, then filters, and 30-50 DEG C of desolventizing of filtrate obtains grease, and repetitive cycling is used after the normal temperature compressed liquefaction of solvent n-butane, and filter residue is silkworm chrysalis skimmed milk；

（2）By step（1）The filter residue for obtaining i.e. silkworm chrysalis skimmed milk adds worm silty amount 3-5 NaOH solution again, 10-60min is crushed with colloid mill, then extracts 20~30min with energy-collecting ultrasonic circulation all-in-one machine, is then centrifuged with 3800~4000g of horizontal screw centrifuge, makes residue and protein solution separation, and residue is used for chitin and produces or prepare；

（3）By step（2）The residue for obtaining, is soaked, using energy-collecting ultrasonic circulation all-in-one machine decalcification 25 with the HCl of 10~30 mmol/l ~30min, then be centrifuged with 3800~4000g of horizontal screw centrifuge, filter residue is decalcification worm shell, i.e. chitin；

（4）By step（3）The spray-dried device spray drying of filter residue of gained obtains chitin.

Present device is simple, and simple to operate, preparation condition is easily controllable, and is suitable to large-scale production.

Specific embodiment

Embodiment 1：A kind of extraction process of silkworm chrysalis chitin, comprises the following steps：

（1）Silkworm chrysalis is cleared up into removal of impurities, microwave 10kg/KWh is dried, pulverizer adds the normal butane of 3 times of dried silkworm chrysalis meal quality after crushing, 1.5Bar extractions 20min in 30 DEG C of subcritical abstraction tanks, then filter, 30 DEG C of desolventizings of filtrate obtain grease, and repetitive cycling is used after the normal temperature compressed liquefaction of solvent n-butane, and filter residue is silkworm chrysalis skimmed milk；

（2）By step（1）The filter residue for obtaining i.e. silkworm chrysalis skimmed milk adds worm silty amount 3 NaOH solution again, 10min is crushed with colloid mill, then extracts 20min with energy-collecting ultrasonic circulation all-in-one machine, then uses horizontal screw centrifuge 3800g Centrifugation, separates residue and protein solution, and residue is used for chitin and produces or prepare；

（3）By step（2）The residue for obtaining, is soaked with the HCl of 10mmol/L, using energy-collecting ultrasonic circulation all-in-one machine decalcification 25min, then is centrifuged with horizontal screw centrifuge 3800g, and filter residue is decalcification worm shell, i.e. chitin；

（4）By step（3）The spray-dried device spray drying of filter residue for obtaining（165 DEG C of feeding mouth temperature, 80 DEG C of outlet temperature）Obtain chitin.

Embodiment 2：A kind of extraction process of silkworm chrysalis chitin, comprises the following steps：

（1）Silkworm chrysalis is cleared up into removal of impurities, microwave 13kg/KWh is dried, pulverizer adds the normal butane of 4 times of dried silkworm chrysalis meal quality after crushing, 1.6Bar extractions 30min in 33 DEG C of subcritical abstraction tanks, then filter, 40 DEG C of desolventizings of filtrate obtain grease, and repetitive cycling is used after the normal temperature compressed liquefaction of solvent n-butane, and filter residue is silkworm chrysalis skimmed milk；

（2）By step（1）The filter residue for obtaining i.e. silkworm chrysalis skimmed milk adds worm silty amount 4 NaOH solution again, 30min is crushed with colloid mill, then extracts 25min with energy-collecting ultrasonic circulation all-in-one machine, then uses horizontal screw centrifuge 3900g Centrifugation, separates residue and protein solution, and residue is used for chitin and produces or prepare；

（3）By step（2）The residue for obtaining, is soaked with the HCl of 20mmol/L, using energy-collecting ultrasonic circulation all-in-one machine decalcification 28min, then is centrifuged with horizontal screw centrifuge 3900g, and filter residue is decalcification worm shell, i.e. chitin；

（4）By step（3）The spray drying of filter residue spray-dried device（170 DEG C of feeding mouth temperature, 85 DEG C of outlet temperature）Obtain chitin.

Embodiment 3：A kind of extraction process of silkworm chrysalis chitin, comprises the following steps：

（1）Silkworm chrysalis is cleared up into removal of impurities, microwave 15kg/KWh is dried, pulverizer adds the normal butane of 5 times of dried silkworm chrysalis meal quality after crushing, 1.8Bar extractions 40min in 36 DEG C of subcritical abstraction tanks, then filter, 50 DEG C of desolventizings of filtrate obtain grease, and repetitive cycling is used after the normal temperature compressed liquefaction of solvent n-butane, and filter residue is silkworm chrysalis skimmed milk；

（2）By step（1）The filter residue for obtaining i.e. silkworm chrysalis skimmed milk adds worm silty amount 5 NaOH solution again, 60min is crushed with colloid mill, then extracts 30min with energy-collecting ultrasonic circulation all-in-one machine, then uses horizontal screw centrifuge 4000g Centrifugation, separates residue and protein solution, and residue is used for chitin and produces or prepare；

（3）By step（2）The residue for obtaining, is soaked with the HCl of 30mmol/L, using energy-collecting ultrasonic circulation all-in-one machine decalcification 30min, then is centrifuged with horizontal screw centrifuge 4000g, and filter residue is decalcification worm shell, i.e. chitin；

（4）By step（3）The spray-dried device spray drying of filter residue for obtaining（180 DEG C of feeding mouth temperature, 90 DEG C of outlet temperature）Obtain chitin.

Embodiment 4：A kind of extraction process of silkworm chrysalis chitin, comprises the following steps：

（1）Silkworm chrysalis is cleared up into removal of impurities, microwave 15kg/KWh is dried, pulverizer adds the normal butane of 3 times of dried silkworm chrysalis meal quality after crushing, 1.7Bar extractions 30min in 34 DEG C of subcritical abstraction tanks, then filter, 50 DEG C of desolventizings of filtrate obtain grease, and repetitive cycling is used after the normal temperature compressed liquefaction of solvent n-butane, and filter residue is silkworm chrysalis skimmed milk；

（2）By step（1）The filter residue for obtaining i.e. silkworm chrysalis skimmed milk adds worm silty amount 3 NaOH solution again, 40min is crushed with colloid mill, then extracts 28min with energy-collecting ultrasonic circulation all-in-one machine, then uses horizontal screw centrifuge 4000g Centrifugation, separates residue and protein solution, and residue is used for chitin and produces or prepare；

（3）By step（2）The residue for obtaining, is soaked with the HCl of 25mmol/L, using energy-collecting ultrasonic circulation all-in-one machine decalcification 25min, then is centrifuged with horizontal screw centrifuge 3800g, and filter residue is decalcification worm shell, i.e. chitin；

（4）By step（3）The spray-dried device spray drying of filter residue for obtaining（180 DEG C of feeding mouth temperature, 88 DEG C of outlet temperature）Obtain chitin.

The present invention uses subcritical abstraction technology scale degreasing to silkworm chrysalis powder first, has reached efficient, energy-conservation, zero-emission effect.Using energy-collecting ultrasonic cycling extraction technology, the fairly large de- albumen of silkworm chrysalis skimmed milk, decalcification are not only realized, while having reached efficient, energy-conservation, low emission effect.Using sleeping spiral shell centrifugation technique, feed liquid de- albumen, depickling alkali efficiency are not only increased, and improve the chitin rate of recovery.The present invention provide not only a kind of technical matters of the large-scale production silkworm chrysalis chitin of simplicity, while also having expanded silkworm chrysalis comprehensive exploitation value.Present device is simple, and simple to operate, preparation condition is easily controllable, and is suitable to large-scale production.

Claims (2)

1. a kind of extraction process of silkworm chrysalis chitin, it is characterised in that：Comprise the following steps：

（1）Silkworm chrysalis is cleared up into removal of impurities, 10~15kg/KWh of microwave is dried, pulverizer adds the normal butane of 3-5 times of dried silkworm chrysalis meal quality after crushing, 1.5~1.8Bar extractions 20-40min in 30~36 DEG C of subcritical abstraction tanks, then filter, 30-50 DEG C of desolventizing of filtrate obtains grease, and repetitive cycling is used after the normal temperature compressed liquefaction of solvent n-butane, and filter residue is silkworm chrysalis skimmed milk；

（2）By step（1）The filter residue for obtaining i.e. silkworm chrysalis skimmed milk adds the NaOH solution of 3-5 times of worm silty amount, and 10-60min is crushed with colloid mill, then extracts 20~30min with energy-collecting ultrasonic circulation all-in-one machine, then with horizontal screw centrifuge 3800 ~4000g is centrifuged, and separates residue and protein solution, and residue is used for chitin and produces or prepare；

（3）By step（2）The residue for obtaining, is soaked with the HCl of 10~30 mmol/l, using energy-collecting ultrasonic circulation all-in-one machine decalcification 25~ 30min, then be centrifuged with 3800~4000g of horizontal screw centrifuge, filter residue is decalcification worm shell, i.e. chitin；

（4）By step（3）The spray-dried device spray drying of filter residue of gained obtains chitin.

2. the extraction process of a kind of silkworm chrysalis chitin according to claim 1, it is characterised in that：Spray dryer described in step (4) dries 165~180 DEG C of feeding mouth temperature, 80~90 DEG C of outlet temperature.