CN1068473A - Large spore ear milddew and to the control of aphid - Google Patents
Large spore ear milddew and to the control of aphid Download PDFInfo
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- CN1068473A CN1068473A CN 92103608 CN92103608A CN1068473A CN 1068473 A CN1068473 A CN 1068473A CN 92103608 CN92103608 CN 92103608 CN 92103608 A CN92103608 A CN 92103608A CN 1068473 A CN1068473 A CN 1068473A
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- milddew
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Abstract
The present invention relates to a kind of large spore ear milddew and utilize the control of large spore ear milddew, particularly to the control of tobacco aphid aphid and acarid.Large spore ear milddew of the present invention is the new bacterial strain of large spore ear milddew that separation and Culture goes out in a kind of aphid body of falling ill from tobacco, and this bacterial strain now has been stored in CCMCC, preserving number: 170, preservation date: 92.1.23.The fermented extracted method of large spore ear milddew desinsection thing of the present invention (being its conidium, resting spore, mycelium) is general microbial fermentation extraction method, and equipment and incubation that it is required are simple, and killing rate can reach more than 90%, and stores easily.
Description
The present invention relates to a kind of large spore ear milddew and utilize the control of large spore ear milddew, particularly to the control of tobacco aphid aphid and acarid.
Characteristics such as cigarette aphid [Mygus Persicae(Sulger)] is the same with other aphid, and it is short to have a life cycle, and fertility is strong, and generation overlap is serious, and parasitic scope is wide.The cigarette aphid is clustered on the tender leaf of cigarette strain top causes harm, and causes cigarette strain poor growth, and leaf rolling, and the viral disease that spreads disease reduce tobacco production, and the product qualitative change is bad, and severe patient makes whole strain tobacco necrosis.At present,, the natural enemy of aphid is come to harm, reduced cigarette quality, polluted environment, and be detrimental to health because single use chemical pesticide control causes pest resistance to insecticide to strengthen.
The object of the present invention is to provide a kind of biotic factor that utilizes, promptly large spore ear milddew is prevented and treated the cigarette aphid, brings into play the overall efficiency of natural enemy of aphid in the vega simultaneously, reaches to reduce the harmful effect of residual toxicity to cigarette quality.
I is the conidium of large spore ear milddew " 88-I " among Fig. 1, and I is the resting spore of 88-I.
It among Fig. 2 the colony characteristics of 88-I.
Among Fig. 3 the resting spore of 88-I on the aphid corpse.
Fig. 4 is the rhizoid of 88-I.
Fig. 5 is the rhizoid of the large spore ear milddew on the parasitic brown planthopper analogy.
One, the separation screening of bacterial strain and definite
1, the separation of bacterial classification and guarantor's root of Dahurian angelica:
From vega, choose the fresh luteotestaceous aphid corpse that is infected by large spore ear milddew, take back in the laboratory, behind the natural air drying, one with insect needle sticks in aphid, insert in the plating medium that has prepared sterilization back, other end, make the aphid corpse stand on medium above, and be placed in 25 ℃ of insulating boxs, the spore scattering for the treatment of large spore ear milddew is in medium, the spore that the picking edge does not have an assorted bacterium under aseptic condition is transferred and is cultivated in another plating medium, thereby obtains effective large spore ear milddew [(Conidiobolws majos Rem.et kell.) different name (Entomophthora major Gustafs bacterial strain.This bacterial strain is named as 88-I bacterial strain, adopts the inclined-plane original seed to be stored in 4 ℃ the refrigerator, and per 2 weeks switching once.
2, the biological property of 88-I bacterial strain:
Aphid dark yellow, no cystidium, rhizoid monofilament shape (as Fig. 4).Nascent conidium sphere, mastoid process is (as Fig. 1) obviously, and size is 41-63 * 30-57Nm, average 50 * 46Nm.Resting spore sphere (as Fig. 1), outer wall is smooth, and diameter is 52-70Nm, average out to 62Nm, wall thickness 2.5-6.5Nm.
The conidium size that derives from the potato synthetic medium is 20-72 * 16-63Nm, average out to 46 * 38Nm, and the resting spore size is 19-79Nm, average out to 46Nm.
3, the mycology feature and the physiological and biochemical property of 88-I bacterial strain:
Bacterium colony is grown on the potato synthetic medium rapidly, and the bacterium colony surface has netted fold lines, and like the fishnet shape, the bacterium colony initial stage is an ecru, and the later stage is a milk yellow.Large spore ear milddew is not remarkable to the utilization of sugar, and it shows as soluble starch＞sucrose＞glucose in proper order to the utilization of sugar.Large spore ear milddew is having N, all can grow on the medium in C source, but peptone, yeast extract, living good when yolk exists are arranged, and it to the utilization in N source is in proper order: peptone＞yeast extract＞fish meal＞analysis for soybean powder.The pH value scope of large spore ear milddew growth and product spore is at 4.5-9.5, and is wherein comparatively suitable with 6.0-7.5, with 6.5 the bests.The temperature range of its growth is 10-40 ℃, and is wherein comparatively suitable with 20-30 ℃, with 30 ℃ of the bests.
4,88-I bacterial strain and the difference that parasitizes the biological property of the large spore ear milddew on the brown planthopper analogy:
A, 88-I bacterial strain
(1) all produces resting spore (as Fig. 1, as Fig. 3) on aphid corpse and the medium
(2) rhizoid monofilament shape, no sucker (as Fig. 4)
B, parasitize the large spore ear milddew on the brown planthopper analogy:
(1) all there is not resting spore on aphid corpse and the medium
(2) rhizoid has sucker (as Fig. 5)
Therefore 88-I bacterial strain is the new bacterial strain of large spore ear milddew.This bacterial strain has now been protected the root of Dahurian angelica and has been protected root of Dahurian angelica administration committee common micro-organisms center at Chinese microorganism strain, protects the root of Dahurian angelica number to be: CGMCC NO.170
Two, the cultivation of desinsection thing and extraction
Toxin that the present invention is the conidium that utilizes large spore ear milddew, resting spore and mycelium produced and their infectivity are prevented and treated all kinds of aphids and mite class.We are referred to as the desinsection thing with conidium, resting spore and the mycelium of large spore ear milddew.Its concrete extraction process process of cultivating is as follows.
(1) slant strains:
Preserve bacterial classification with potato synthetic medium or conventional medium (as sabouraud medium PDA medium), bacterial classification places 4～5 ℃ refrigerator, and storage life was 2～6 weeks, if adopt low temperature (20 ℃) refrigeration to preserve, can preserve more than 10.
(2) the dull and stereotyped cultivation
Make plating medium with the potato synthetic medium, connect bacterium after, under the temperature about 20～35 ℃, cultivated 2-4 days, wherein cultivate 2-3 days the bests with 28 ℃ of left and right sides conditions.
(3) conical flask shaken cultivation
Be sub-packed in (50ml or 30ml) in the conical flask with the comprehensive liquid nutrient medium of potato (not adding agar), the amount of every bottling liquid medium is 1/3 of a bottle capacity, insert 0.1% the bacterial classification that derives from plating medium then, under 20～30 ℃ of conditions, cultivated 2～5 days, wherein cultivate 2-3 days the bests with 28 ℃ of conditions, the postvaccinal number of oscillation of conical flask is 90～180 times/minute.
(4) fermented and cultured
Add bean powder (or tapioca flour) with fish meal and make liquid nutrient medium, its prescription is:
Fish meal 2%+ bean powder 2%(or tapioca flour 8%), all the other are sterile water, cultivate 48～72 hours under 25～30 ℃ of temperature in this medium.
(5) recovery of desinsection thing and drying
With plate basket filtration method the filtrate in the above-mentioned zymotic fluid is separated with filter residue, filter residue is that 20 °～30 ℃ relative temperatures are natural seasoning under 80～90% the condition in temperature, get final product the pure powder of desinsection thing.Every 100ml culture can obtain the pure mycelia of 5g, and mycelium can obtain the pure powder of 2g spore under above-mentioned condition, and it is 4,000,000,000 that every gram spore contains the spore amount.
(6) application of desinsection thing and result of use thereof
Add after its insecticidal effect economical and effective of spray that the pure powder of 0.4+g makes sprays desinsection thing suspension with per 20 kg of water, aphid promptly stops to get food, presents dysphoria, beginning is dead after 2-3 hour, the aphid lethality can reach more than 60% in 24 hours, and in 72 hours, lethality can reach more than 90%.
The prescription of above-mentioned potato synthetic medium is: potato 40g+ peptone 2g+ yeast extract 2g+ glucose 20g+ water 100ml(solid culture medium adds 18-20g agar).
The present invention has the advantage of following several respects:
(1) production technology is simple: it is simple to produce large spore ear milddew desinsection thing required equipment and incubation.
(2) good disinsection effect and stable in 72 hours, can reach more than 90% the killing rate of aphid and acarid.
(3) the desinsection thing is stored easily.
(4), the natural enemy of aphid there is not the effect that kills and wounds to the warm blooded animal safety non-toxic.
(5) reduce of the harmful effect of residue of pesticide residual hazard, can increase foreign exchange earnings from exports tobacco and the generation of other crops.
(6) protection environment, the protection ecological balance guarantees health.
Claims (1)
1, the fermented extracted method (the used large spore ear milddew of this method is that separation screening obtains in the aphid body of falling ill from vega) of a kind of large spore ear milddew (be stored in CGMCC.NO.170 and protect root of Dahurian angelica date 92.1.23) desinsection thing (being conidium, resting spore, the mycelium of large spore ear milddew) is general microbial fermentation extracting method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92103608 CN1068473A (en) | 1992-05-13 | 1992-05-13 | Large spore ear milddew and to the control of aphid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92103608 CN1068473A (en) | 1992-05-13 | 1992-05-13 | Large spore ear milddew and to the control of aphid |
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| Publication Number | Publication Date |
|---|---|
| CN1068473A true CN1068473A (en) | 1993-02-03 |
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| CN 92103608 Pending CN1068473A (en) | 1992-05-13 | 1992-05-13 | Large spore ear milddew and to the control of aphid |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012025270A2 (en) | 2010-08-23 | 2012-03-01 | Universite De Liege | Use of a composition as an auxiliary attractant |
| CN104059856A (en) * | 2014-06-20 | 2014-09-24 | 肖关丽 | Conidiobolus coronatus TH130914 and application thereof |
| CN105087393A (en) * | 2015-08-25 | 2015-11-25 | 云南省烟草公司大理州公司 | Conidiobolus pseudococci CPMD 140813 and application thereof |
| CN105794859A (en) * | 2013-01-25 | 2016-07-27 | 陕西汤普森生物科技有限公司 | Insecticidal compound containing conidioblous thromboides and biological sources |
| CN106818672A (en) * | 2016-12-27 | 2017-06-13 | 重庆维得鲜农业发展有限公司 | The method for preventing and treating FLOS CHRYSANTHEMI ALBA from Haizhou of China aphid insect pest |
-
1992
- 1992-05-13 CN CN 92103608 patent/CN1068473A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012025270A2 (en) | 2010-08-23 | 2012-03-01 | Universite De Liege | Use of a composition as an auxiliary attractant |
| CN105794859A (en) * | 2013-01-25 | 2016-07-27 | 陕西汤普森生物科技有限公司 | Insecticidal compound containing conidioblous thromboides and biological sources |
| CN105794859B (en) * | 2013-01-25 | 2018-11-20 | 陕西汤普森生物科技有限公司 | A kind of Pesticidal combination containing Conidiobolus thromboides and biogenic substances |
| CN104059856A (en) * | 2014-06-20 | 2014-09-24 | 肖关丽 | Conidiobolus coronatus TH130914 and application thereof |
| CN104059856B (en) * | 2014-06-20 | 2016-08-24 | 云南农业大学 | A kind of hat mould TH130914 of ear and application thereof |
| CN105087393A (en) * | 2015-08-25 | 2015-11-25 | 云南省烟草公司大理州公司 | Conidiobolus pseudococci CPMD 140813 and application thereof |
| CN105087393B (en) * | 2015-08-25 | 2018-12-14 | 云南省烟草公司大理州公司 | A kind of mould CPMD140813 of mealybug ear and its application |
| CN106818672A (en) * | 2016-12-27 | 2017-06-13 | 重庆维得鲜农业发展有限公司 | The method for preventing and treating FLOS CHRYSANTHEMI ALBA from Haizhou of China aphid insect pest |
| CN106818672B (en) * | 2016-12-27 | 2020-06-05 | 重庆维得鲜农业发展有限公司 | Method for preventing and treating aphid pests of Hangzhou white chrysanthemum |
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