CN106841151A - The detection method of micro-fluidic chip and its micro-channel structure and liquid droplet - Google Patents
The detection method of micro-fluidic chip and its micro-channel structure and liquid droplet Download PDFInfo
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- CN106841151A CN106841151A CN201710166150.3A CN201710166150A CN106841151A CN 106841151 A CN106841151 A CN 106841151A CN 201710166150 A CN201710166150 A CN 201710166150A CN 106841151 A CN106841151 A CN 106841151A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/087—Multiple sequential chambers
Abstract
The invention discloses a kind of micro-fluidic chip and its micro-channel structure and liquid droplet detection method.The micro-channel structure includes sample introduction fluid channel, the first dilution fluid channel, the second dilution fluid channel, in line sequence fluid channel, balanced sorting fluid channel and detection fluid channel.The micro-fluidic chip and its micro-channel structure, by before in line sequence fluid channel is located at into balanced sorting fluid channel, and set first in the entrance of in line sequence fluid channel and dilute fluid channel and the second dilution fluid channel, the unordered and intensive liquid droplet group for thus entering from sample introduction fluid channel forms substantially rectilinear arrangement in line sequence fluid channel after diluted by preliminarily diluted rectification, enter balanced sorting fluid channel by the liquid droplet of preliminary rectification afterwards, follow-up detection fluid channel can equably be entered with identical spacing after balanced sorting fluid channel rectification, avoid liquid droplet from deviateing location mid-shaft to impact follow-up testing result, ensure the accuracy of testing result.
Description
Technical field
The present invention relates to biochemistry detection field, more particularly, to a kind of micro-fluidic chip and its miniflow for fluoroscopic examination
Road structure and liquid droplet detection method.
Background technology
The accuracy of detection of digital pcr detection means requirement is very high, it is desirable to be able to accurately detect that each passes through detection channel
Liquid droplet included in fluorescence signal size, therefore it requires that liquid droplet size is consistent and gap is equal between droplet
It is even.Existing detection chip is typically only diluted liquid arrangement, when droplet size is less than chip channel size, its arrangement effect
It is poor, it is often necessary to go to change chip because droplet size changes.
The patent Shen of Application No. 201610421625.4, a kind of entitled micro flow control chip device and its micro-channel structure
Please in disclose the micro-fluidic chip that a kind of utilization Dien stream is focused on.The micro-fluidic chip uses asymmetric focusing runner, and
Before asymmetric focusing runner is located at straight focusing runner.When in use, first there is particle to injection in asymmetric focusing runner
Sample solution, sample solution directly focuses on runner by entering after asymmetric focusing runner, at this moment, due in asymmetric focusing runner
In acted on by inertia lifting force and Dien drag, in sample solution the particle of different-grain diameter be respectively self-aligned into one it is straight
Line, and because the equilbrium position of inertia lifting force and Dien drag is near the outside of outer curve and the inner side of inner curve, institute
To flow out the particle queue of asymmetric focusing bend not on straight focus channel center line, but meeting disalignment is near straight
The side of focus channel；At the same time, to sheath fluid is injected in sheath fluid runner, sheath fluid is divided into two strands, respectively from left runner and the right side
Runner imports straight focusing runner, under the focussing force of left and right sheath fluid stream, can all focus on directly the particle of different-grain diameter
Focus on the center line of runner.
The Dien stream of above-mentioned micro-fluidic chip focused on runner before straight focusing runner, and sample diluting liquid is in rear addition,
Liquid droplet is not particularly suited for, particularly when liquid droplet size and chip structure size are not corresponded, is held very much after being diluted
Multiple liquid droplets are also easy to produce side by side by runner or the inhomogenous phenomenon of dilution, and M waveforms or signal can be produced in detection
Strength Changes are too big, once and the change of liquid droplet size, generally require to spend high cost to go to make new corresponding core
Piece.
The content of the invention
Based on this, it is necessary to provide one kind and be applied to liquid droplet and liquid droplet can be made equably to enter detection channel
Micro-fluidic chip and its micro-channel structure and liquid droplet detection method.
A kind of micro-channel structure, including sample introduction fluid channel, the first dilution fluid channel, the second dilution fluid channel, in line sequence are micro-
Runner, balanced sorting fluid channel and detection fluid channel；Wherein, the first dilution fluid channel and the second dilution fluid channel exist
The both sides of the sample introduction fluid channel intersect in exit with the sample introduction fluid channel and connect；The entrance of the in line sequence fluid channel
With the outlet of the sample introduction fluid channel；The entrance of the balanced sorting fluid channel connects with the outlet of the in line sequence fluid channel
Logical, the balanced sorting fluid channel has multiple identical Pipe Elbow Elements, and multiple identical Pipe Elbow Elements are sequentially communicated and adjacent
Pipe Elbow Element bending direction it is opposite；The entrance of the detection fluid channel and the outlet of the balanced sorting fluid channel.
Wherein in one embodiment, the size of the size more than its port of export of the arrival end of the sample introduction fluid channel, and
The sample introduction fluid channel is gradually narrowed near its port of export.
Wherein in one embodiment, the first dilution fluid channel and the second dilution fluid channel are micro- with the sample introduction
Formed angle is identical between runner.
Wherein in one embodiment, the first dilution fluid channel and the second dilution fluid channel are micro- with the sample introduction
The number of degrees of formed angle are 45 °~90 ° between runner.
Wherein in one embodiment, the Pipe Elbow Element is semicircle.
Wherein in one embodiment, the radius of curvature of the Pipe Elbow Element is 50 μm~300 μm.
Wherein in one embodiment, the sample introduction fluid channel, the first dilution fluid channel, the second dilution miniflow
The cross section of road, the in line sequence fluid channel, the balanced sorting fluid channel and the detection fluid channel is rectangle or is
It is circular.
Wherein in one embodiment, the sample introduction fluid channel, the first dilution fluid channel, the second dilution miniflow
The cross section of road, the in line sequence fluid channel, the balanced sorting fluid channel and the detection fluid channel is rectangle, and height
20 μm~150 μm are with width.
A kind of micro-fluidic chip, including substrate and covering cover plate on the substrate, wherein, the substrate is provided with above-mentioned
Micro-channel structure described in any embodiment, the cover plate is provided with the injection port and described connected with the sample introduction fluid channel
The first dilution entrance and described second the second dilution entrance for connecting of dilution fluid channel of one dilution fluid channel connection and
The liquid outlet connected with the detection fluid channel.
A kind of detection method of liquid droplet, using above-mentioned micro-fluidic chip, the detection method comprises the following steps：
The sample solution containing liquid droplet, liquid droplet is added to enter the sample introduction fluid channel from the injection port, together
When each lead into dilution from the first dilution entrance and the second dilution entrance, liquid droplet is through the dilution
Enter by entering the balanced sorting fluid channel after preliminarily diluted rectification in the in line sequence fluid channel, by described after dilution
Liquid droplet after balanced sorting fluid channel forms the consistent liquid droplet to be detected of arranged in a straight line and spacing and enters detection miniflow
Road；
The liquid droplet to be detected is detected in the test position of the detection fluid channel.
Above-mentioned micro-fluidic chip and its micro-channel structure, by by line sequence fluid channel located at balanced sorting fluid channel it
Before, and entrance in line sequence fluid channel sets first and dilutes fluid channel and the second dilution fluid channel, thus from sample introduction miniflow
The unordered and intensive liquid droplet group entered in road is whole by preliminarily diluted in line sequence fluid channel after diluted
Stream forms substantially rectilinear arrangement, but liquid droplet now is not perfectly straight form, due to the size of liquid droplet
It is likely less than or more than the size of passage, liquid droplet may be uneven due to what is flowed after being sorted by dilution fluid channel
And produce pass through side by side, the phenomenon such as spacing differs, drop is stretched, these phenomenons can cause to be produced in detection and be difficult to expect
Waveform signal, and balanced sorting fluid channel afterwards can just solve these problems, afterwards, by the liquid droplet of preliminary rectification
Into balanced sorting fluid channel, the secondary vortex flow that the centrifugal force that liquid is produced when being moved in balanced sorting fluid channel triggers is to molten
Liquid droplet in liquid applies Dien drag force, while the liquid droplet in solution is also by the inertia lifting force of inertia flow, this two
The balance for planting power causes that liquid droplet fixed position in fluid channel reaches balance, because balanced sorting fluid channel has multiple identical
Pipe Elbow Element, and adjacent Pipe Elbow Element bending direction conversely, thus liquid droplet stably can be maintained at into miniflow
The location mid-shaft in road and with identical spacing equably enter follow-up detection fluid channel, it is to avoid liquid droplet deviate location mid-shaft
Follow-up testing result is impacted, it is ensured that the accuracy of testing result.Single balanced sorting fluid channel will be symmetrical
Sequence fluid channel does not reach preferably sequence effect before being arranged on the in line sequence fluid channel of dilution rectification, because of liquid droplet
The reason for size, it is constantly present liquid droplet spacing and differs, is stretched or produces the problem for passing through side by side, it is impossible to ensures detection
The accuracy of result.
Brief description of the drawings
Fig. 1 is the structural representation of the micro-channel structure of an embodiment of the present invention；
Fig. 2 is that in the in line sequence fluid channel of Fig. 1, the arranging situation through the liquid droplet after preliminarily diluted rectification is illustrated
Figure；
Fig. 3 is that in the detection fluid channel of Fig. 1, the arranging situation through the liquid droplet after balanced sorting fluid channel rectification shows
It is intended to.
Specific embodiment
For the ease of understanding the present invention, the present invention is described more fully below with reference to relevant drawings.In accompanying drawing
Give presently preferred embodiments of the present invention.But, the present invention can be realized in many different forms, however it is not limited to this paper institutes
The embodiment of description.On the contrary, the purpose for providing these embodiments is to make the understanding to the disclosure more thorough
Comprehensively.
It should be noted that when element is referred to as " being fixed on " another element, it can directly on another element
Or can also there is element placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or may be simultaneously present centering elements.
Unless otherwise defined, all of technologies and scientific terms used here by the article with belong to technical field of the invention
The implication that technical staff is generally understood that is identical.The term for being used in the description of the invention herein is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term as used herein "and/or" includes one or more phases
The arbitrary and all of combination of the Listed Items of pass.
As shown in figure 1, the micro-channel structure 100 of an embodiment of the present invention includes that the dilution of sample introduction fluid channel 110, first is micro-
The dilution of runner 120, second fluid channel 130, in line sequence fluid channel 140, balanced sorting fluid channel 150 and detection fluid channel 160.
Sample introduction fluid channel 110 is used for sample introduction, and its entrance is used to be connected with sampling pump, and liquid droplet will be contained by sampling pump
Sample solution pump into.
Wherein in some implementation methods, the size of the size more than its port of export of the arrival end of sample introduction fluid channel 110.Enter
Sample fluid channel 110 is gradually narrowed near its port of export, to make liquid droplet pass through in single form as far as possible.
The entrance of the first dilution dilution fluid channel 130 of fluid channel 120 and second is connected with dilution pumps, will be dilute by dilution pumps
Liquid pump is released to enter.In diagrammatically shown implementation method, the first dilution fluid channel 120 and second dilutes fluid channel 130 in sample introduction miniflow
The both sides in road 110 intersect in exit with sample introduction fluid channel 110 and connect.
Wherein in some implementation methods, the first dilution fluid channel 120 and second dilutes fluid channel 130 and sample introduction fluid channel
Formed angle is identical between 110.Further, in some embodiments, the first dilution dilution miniflow of fluid channel 120 and second
The number of degrees of formed angle are 45 °~90 ° between road 130 and sample introduction fluid channel 110.Further, in some embodiments,
The number of degrees of formed angle are 45 ° between first dilution fluid channel 120 and second dilution fluid channel 130 and sample introduction fluid channel 110.
The entrance of in line sequence fluid channel 140 and the outlet of sample introduction fluid channel 110.In line sequence fluid channel 140 is straight tube
Shape.Wherein in some implementation methods, in line sequence fluid channel 140 is that axis is set altogether with sample introduction fluid channel 110.
The outlet of the entrance of balanced sorting fluid channel 150 and in line sequence fluid channel 140.Balanced sorting fluid channel 150
With multiple identical Pipe Elbow Elements 152.Multiple identical Pipe Elbow Elements 152 are sequentially communicated and adjacent Pipe Elbow Element 152
Bending direction is opposite.
Wherein in some implementation methods, Pipe Elbow Element 152 is semicircle.In some embodiments, Pipe Elbow Element
Radius of curvature is 50 μm~300 μm.In other embodiments, Pipe Elbow Element 152 is not limited to semicircular structure, such as balanced sorting
Fluid channel 150 can also be sinusoidal waveform etc., as long as balanced sorting fluid channel 150 is sequentially communicated with multiple identicals and phase
The opposite Pipe Elbow Element 152 of adjacent bending direction.
Detect the entrance of fluid channel 160 and the outlet of balanced sorting fluid channel 150.Detection fluid channel 160 is used to supply
Testing equipment detects the status information of liquid droplet therein.
Wherein in some implementation methods, the dilution dilution fluid channel of fluid channel 120, second of sample introduction fluid channel 110, first
130th, the cross section of in line sequence fluid channel 140, balanced sorting fluid channel 150 and detection fluid channel 160 is rectangle or is circle
Shape.Further, the height of each fluid channel is identical with width in some embodiments.Further, in some embodiments,
The cross section of each fluid channel is rectangle, and height and width are 20 μm~150 μm.
Present invention also offers a kind of micro-fluidic chip, it includes substrate and the cover plate being covered on substrate.Wherein, substrate
It is provided with the micro-channel structure 100 of any of the above-described implementation method.Each fluid channel can be opened in base using chemical etching or the method for photoetching
On piece.Cover plate is provided with the first dilution that the injection port connected with sample introduction fluid channel 110 is connected with the first dilution fluid channel 120
The liquid outlet that entrance connects 130 the second dilution entrance with the second dilution fluid channel and connected with detection fluid channel 160.
Additionally, the micro-fluidic chip also corresponds to detection fluid channel 160 on cover plate is provided with detection window, for for testing equipment inspection
Survey.
Further, present invention also offers a kind of detection method of liquid droplet, using above-mentioned micro-fluidic chip 100, should
Detection method comprises the following steps：
The sample solution containing liquid droplet, liquid droplet is added to enter sample introduction fluid channel 110 from injection port, while from the
One dilution entrance and the second dilution entrance each lead into dilution, and it is micro- that liquid droplet enters in line sequence after diluted
By entering balanced sorting fluid channel 150, the liquid after balanced sorting fluid channel 150 after preliminarily diluted rectification in runner 140
State droplet forms the consistent liquid droplet to be detected of arranged in a straight line and spacing and enters detection fluid channel 160；
Liquid droplet to be detected is detected in the test position of detection fluid channel 160.
Above-mentioned micro-fluidic chip and its micro-channel structure 100, balanced sorting miniflow is located at by by line sequence fluid channel 140
Before road 150, and in entrance setting the first dilution dilution fluid channel of fluid channel 120 and second of in line sequence fluid channel 140
130, thus the unordered and intensive liquid droplet group entered from sample introduction fluid channel 110 is micro- in line sequence after diluted
Form substantially rectilinear arrangement by preliminarily diluted rectification in runner 140, as shown in Fig. 2 but liquid droplet now be not
Perfectly straight form, is likely less than or more than the size of passage due to the size of liquid droplet, and liquid droplet is passing through dilution
May be produced due to the uneven of flowing after the sequence of liquid fluid channel pass through side by side, the phenomenon such as spacing differs, drop is stretched, this
A little phenomenons can be caused to produce the waveform signal for being difficult to expect in detection, and balanced sorting fluid channel 150 afterwards can just be solved
Certainly these problems, afterwards, the liquid droplet by preliminary rectification enters balanced sorting fluid channel 150, and liquid is micro- in balanced sorting
The secondary vortex flow that the centrifugal force produced when being moved in runner 150 triggers applies Dien drag force to the liquid droplet in solution, together
When solution in liquid droplet also by the inertia lifting force of inertia flow, the balance of both power causes liquid droplet in fluid channel
Fixed position reaches balance, because balanced sorting fluid channel 150 has multiple identical Pipe Elbow Elements 152, and adjacent bend pipe list
Unit 152 bending direction conversely, thus liquid droplet stably can be maintained at the location mid-shaft of fluid channel and with identical
Spacing equably enters follow-up detection fluid channel 160, as shown in Figure 3, it is to avoid liquid droplet deviates location mid-shaft to follow-up
Testing result is impacted, it is ensured that the accuracy of testing result.Single balanced sorting fluid channel 150 or balanced sorting is micro-
Runner 150 does not reach preferably sequence effect before being arranged on the in line sequence fluid channel 140 of dilution rectification, because of liquid droplet
The reason for size, it is constantly present liquid droplet spacing and differs, is stretched or produces the problem for passing through side by side, it is impossible to ensures detection
The accuracy of result.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope of this specification record is all considered to be.
Embodiment described above only expresses several embodiments of the invention, and its description is more specific and detailed, but simultaneously
Can not therefore be construed as limiting the scope of the patent.It should be pointed out that coming for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of micro-channel structure, it is characterised in that including sample introduction fluid channel, the first dilution fluid channel, the second dilution fluid channel,
In line sequence fluid channel, balanced sorting fluid channel and detection fluid channel；Wherein, the first dilution fluid channel and second dilution
Fluid channel is intersected in exit with the sample introduction fluid channel in the both sides of the sample introduction fluid channel and is connected；The in line sequence miniflow
The outlet of the entrance in road and the sample introduction fluid channel；The entrance of the balanced sorting fluid channel and the in line sequence fluid channel
Outlet, the balanced sorting fluid channel has multiple identical Pipe Elbow Elements, and multiple identical Pipe Elbow Elements connect successively
The bending direction of logical and adjacent Pipe Elbow Element is opposite；The detection entrance of fluid channel and the going out for the balanced sorting fluid channel
Mouth connection.
2. micro-channel structure as claimed in claim 1, it is characterised in that the size of the arrival end of the sample introduction fluid channel is more than
The size of its port of export, and the sample introduction fluid channel gradually narrowed near its port of export.
3. micro-channel structure as claimed in claim 1, it is characterised in that the first dilution fluid channel and second dilution
Formed angle is identical between fluid channel and the sample introduction fluid channel.
4. micro-channel structure as claimed in claim 3, it is characterised in that the first dilution fluid channel and second dilution
The number of degrees of formed angle are 45 °~90 ° between fluid channel and the sample introduction fluid channel.
5. micro-channel structure as claimed in claim 1, it is characterised in that the Pipe Elbow Element is semicircle.
6. micro-channel structure as claimed in claim 5, it is characterised in that the radius of curvature of the Pipe Elbow Element is 50 μm~
300μm。
7. micro-channel structure as claimed in claim 1, it is characterised in that the sample introduction fluid channel, the first dilution miniflow
The horizontal stroke of road, the second dilution fluid channel, the in line sequence fluid channel, the balanced sorting fluid channel and the detection fluid channel
Section is rectangle or is circle.
8. micro-channel structure as claimed in claim 7, it is characterised in that the sample introduction fluid channel, the first dilution miniflow
The horizontal stroke of road, the second dilution fluid channel, the in line sequence fluid channel, the balanced sorting fluid channel and the detection fluid channel
Section is rectangle, and height and width are 20 μm~150 μm.
9. a kind of micro-fluidic chip, it is characterised in that including substrate and covering cover plate on the substrate, wherein, the base
Piece is provided with the micro-channel structure as any one of claim 1~8, and the cover plate is provided with and is connected with the sample introduction fluid channel
Injection port dilute what fluid channel was connected with described first the first dilution entrance for connect of dilution fluid channel with described second
Second dilution entrance and the liquid outlet connected with the detection fluid channel.
10. a kind of detection method of liquid droplet, it is characterised in that use micro-fluidic chip as claimed in claim 9, it is described
Detection method comprises the following steps：
The sample solution containing liquid droplet, liquid droplet is added to enter the sample introduction fluid channel from the injection port, while from
The first dilution entrance and the second dilution entrance each lead into dilution, and liquid droplet is through the diluted
Enter afterwards by entering the balanced sorting fluid channel after preliminarily diluted rectification in the in line sequence fluid channel, by described symmetrical
Liquid droplet after sequence fluid channel forms the consistent liquid droplet to be detected of arranged in a straight line and spacing and enters detection fluid channel；
The liquid droplet to be detected is detected in the test position of the detection fluid channel.
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| CN107831811A (en) * | 2017-09-04 | 2018-03-23 | 华南理工大学 | The fluid channel flow control apparatus and control method of a kind of micro-nano cellulose |
| CN108172444A (en) * | 2017-12-28 | 2018-06-15 | 南京理工大学 | A kind of HI high impact inertia microfluid connects electric switch and its manufacturing method |
| CN109746061A (en) * | 2017-11-06 | 2019-05-14 | 北京新羿生物科技有限公司 | Microlayer model generating means |
| CN110605148A (en) * | 2019-10-18 | 2019-12-24 | 广东工业大学 | Micro-channel structure, micro-fluidic chip and quantitative heterogeneous reaction method |
| CN113814008A (en) * | 2020-08-28 | 2021-12-21 | 上海交通大学 | Microfluidic channel structure, chip, particle ordered arrangement method and application |
| CN114717100A (en) * | 2021-07-16 | 2022-07-08 | 墨卓生物科技(浙江)有限公司 | Microfluidic chip for single cell sequencing and application |
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