CN106834120A - A kind of stem cell media nutriment constant basis setter - Google Patents
A kind of stem cell media nutriment constant basis setter Download PDFInfo
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- CN106834120A CN106834120A CN201710171154.0A CN201710171154A CN106834120A CN 106834120 A CN106834120 A CN 106834120A CN 201710171154 A CN201710171154 A CN 201710171154A CN 106834120 A CN106834120 A CN 106834120A
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- left chamber
- right chamber
- air
- chamber
- guide pipe
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
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Abstract
The invention discloses a kind of stem cell media nutriment constant basis setter, including closed container, dividing plate, soft air-guide pipe, sponge；Closed container is divided into left chamber and right chamber room by described dividing plate, and the bottom in next door is by left chamber and right chamber；Described left chamber top is provided with aspirating hole, and aspirating hole is provided with rubber stopper；Air admission hole is provided with the top of described right chamber room, air filtering disinfection device is provided with air admission hole；The bottom of described right chamber room sets liquid outlet, and liquid outlet is connected on culture medium by conduit；Described sponge is floated in left chamber；Described soft air-guide pipe passes through dividing plate, and one end is located in right chamber room, and the other end is arranged at the bottom of sponge；Described soft air-guide pipe is located at height of the total length in left chamber more than left chamber.Design of the invention can ensure that liquid fluctuating is minimum.
Description
Technical field
The present invention relates to a kind of stem cell media nutriment constant basis setter.
Background technology
Stem cell is the multipotential cell that a class has the of self-replication capacity.Under certain condition, it can be divided into many
Plant functioning cell.Stage of development according to residing for stem cell is divided into embryonic stem cell and adult stem cell.According to the hair of stem cell
Potential is educated to be divided three classes：Myeloid-lymphoid stem cell, multipotential stem cell and unipotent stem cell.Stem cell is a kind of inabundant differentiation, still not
Ripe cell, with the potential function for regenerating various histoorgans and human body, medical field is referred to as " general-purpose cell ".
Stem cell can not be survived for a long time in simple basal medium, and some are must provide in cell culture
The nutriment and growth factor of constant basis can just enable cell grow and maintain growth conditions, therefore the basis training for using
Foster base is often required to add serum, and serum is made up of the biomolecule that many sizes are differed, and it can provide cell in body
Required growth factor, hormone and associated proteins during outer culture.Precise control nutriment is needed in cell cultivation process
With the specified rate of growth factor, it can be the device of offer constant basis nutriment in culture medium then to need one.It is existing
Most of the device is all the expensive of import, and a very simple stem cell media of structure was designed before inventor
Nutriment constant basis setter, as shown in Figure 1.The device contains closed container, dividing plate, wireway；Dividing plate will be closed
Container is divided into left and right chamber, and by left and right chamber, left chamber top is provided with aspirating hole, the top of right chamber room for the bottom in next door
Portion is provided with air admission hole, and the bottom of right chamber room sets liquid outlet, and liquid outlet is connected on culture medium by conduit.When right chamber room
Liquid level will once enter into the bottom of left chamber less than the right output port air of wireway by wireway, then by left chamber
Bottom slowly enter into top so that in left chamber pressure increase liquid be flowed into right chamber room so as to ensure right chamber room
Liquid level can maintain certain constant liquid level (height of wireway right output port).It should be noted that the air inlet of right chamber roof
Hole is provided with air disinfection filter, and the aspirating hole at the top of left chamber is provided with rubber stopper, and pumping is extracted by syringe
's.Device shown in Fig. 1 is exactly that air enters into left chamber by the right output port of wireway than larger defect in the presence of one
Bottom, then again by bottom slowly enter into top, whole process slowly, particularly nutrient concentrations it is big if just
It is less useful for air to pass through, so as to cause the liquid fluctuating of right chamber room than larger.
If will be moved in the left port of wireway, the top of the arrival left chamber that such air can be quickly, but problem
Be liquid in left chamber be it is less and less, once the left port of wireway be not be immersed in it is in liquid but completely naked
Dew in atmosphere, can so cause the liquid level of left chamber and right chamber room equal at once (principle of linker), the permanent liquid of right chamber room
Face destroys at once.
The content of the invention
The technical problem to be solved in the present invention is：How to allow that the air in right chamber room rapidly reaches left chamber
Top, while the CL of right chamber room is not deteriorated again.
In order to realize solving above technical problem, the technical solution adopted in the present invention is：A kind of stem cell media battalion
Support material constant basis setter, including closed container, dividing plate, soft air-guide pipe, sponge；
Closed container is divided into the bottom in left chamber and right chamber room, next door to connect left chamber and right chamber room by described dividing plate
It is logical；
Described left chamber top is provided with aspirating hole, and aspirating hole is provided with rubber stopper；
Air admission hole is provided with the top of described right chamber room, air filtering disinfection device is provided with air admission hole；
The bottom of described right chamber room sets liquid outlet, and liquid outlet is connected on culture medium by conduit；
Described sponge is floated in left chamber；
Described soft air-guide pipe passes through dividing plate, and one end is located in right chamber room, and the other end is arranged at the bottom of sponge；
Described soft air-guide pipe is located at height of the total length in left chamber more than left chamber.
Further, the right output port of described soft air-guide pipe is located at 2/3rds height of right chamber room.
Compared with prior art, the advantage of the invention is that：It is very ingenious that the present invention is used, and can both cause wireway
The air that right output port enters can rapidly reach the top of left chamber, will not cause that the constant level in right chamber room is destroyed again, adopt
Can cause that the liquid level of right chamber room is highly stable with the structure of present invention design so that the supply of culture medium nutriment is in unit
It is constant basis in time, fluctuation is not had.
Brief description of the drawings
Fig. 1 is the structural representation of existing stem cell media nutriment constant basis setter；
Fig. 2 is structural representation of the invention.
Wherein, 1 is sponge；2 is soft air-guide pipe.
Specific embodiment
Invention is described in further detail below in conjunction with the accompanying drawings.
Reference picture 2, stem cell media nutriment constant basis setter, including closed container, dividing plate, air guide are soft
Pipe, sponge；Closed container is divided into the bottom in left chamber and right chamber room, next door to connect left chamber and right chamber room by described dividing plate
It is logical；Described left chamber top is provided with aspirating hole, and aspirating hole is provided with rubber stopper；Be provided with the top of described right chamber room into
Stomata, is provided with air filtering disinfection device on air admission hole；The bottom of described right chamber room sets liquid outlet, and liquid outlet is by leading
Pipe is connected on culture medium；Described sponge is floated in left chamber；Described soft air-guide pipe passes through dividing plate, and one end is located at right chamber
Interior, the other end is arranged at the bottom of sponge；Described soft air-guide pipe is located at height of the total length in left chamber more than left chamber
Degree.
Wherein, the right output port of described soft air-guide pipe is located at 2/3rds height of right chamber room.
Operation principle of the invention：Because one end in left chamber of soft air-guide pipe is connected with sponge, so work as a left side
When the liquid level reduction of chamber, one end that soft air-guide pipe is located at left chamber follows sponge to decline together, because sponge is in liquid
Between body and air, the air in such soft air-guide pipe is very easy in the air of the top for entering into left chamber's liquid level, so that
So that the liquid level of right chamber room does not almost fluctuate.
Use the method for present invention addition culture medium nutrient solution：
Step 1), culture medium nutrient solution is extracted using syringe, the right side is then expelled to by the air admission hole of right chamber roof
Chamber interior, when liquid level submergence soft air-guide pipe is located at the port of right chamber room, left chamber Interior Space is extracted using another syringe
Gas, extracts gas while observing the liquid level of right chamber room；
Step 2), when the liquid level of right chamber room is located at the port of right chamber room just below soft air-guide pipe, stop pumping, again
Culture medium nutrient solution is expelled in right chamber room using syringe, is injected while observing the liquid level of right chamber room；
Step 3) when liquid level submerge again soft air-guide pipe positioned at the port of right chamber room when, repeat step 1), step
2), soft air-guide pipe has just been submerged in right chamber room to the liquid level being filled with nutrient solution, and right chamber room in left chamber
During port, stop step 1) and step 2).
Claims (2)
1. a kind of stem cell media nutriment constant basis setter, it is characterised in that including closed container, dividing plate, lead
Gas flexible pipe, sponge；
Closed container is divided into left chamber and right chamber room by described dividing plate, and the bottom in next door is by left chamber and right chamber；
Described left chamber top is provided with aspirating hole, and aspirating hole is provided with rubber stopper；
Air admission hole is provided with the top of described right chamber room, air filtering disinfection device is provided with air admission hole；
The bottom of described right chamber room sets liquid outlet, and liquid outlet is connected on culture medium by conduit；
Described sponge is floated in left chamber；
Described soft air-guide pipe passes through dividing plate, and one end is located in right chamber room, and the other end is arranged at the bottom of sponge；
Described soft air-guide pipe is located at height of the total length in left chamber more than left chamber.
2. stem cell media nutriment constant basis setter according to claim 1, it is characterised in that described
The right output port of soft air-guide pipe is located at 2/3rds height of right chamber room.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710171154.0A CN106834120A (en) | 2017-03-21 | 2017-03-21 | A kind of stem cell media nutriment constant basis setter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710171154.0A CN106834120A (en) | 2017-03-21 | 2017-03-21 | A kind of stem cell media nutriment constant basis setter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106834120A true CN106834120A (en) | 2017-06-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710171154.0A Pending CN106834120A (en) | 2017-03-21 | 2017-03-21 | A kind of stem cell media nutriment constant basis setter |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004089137A (en) * | 2002-09-03 | 2004-03-25 | Olympus Corp | Culture apparatus and method for exchanging culture medium |
| CN203333668U (en) * | 2013-07-04 | 2013-12-11 | 南京博方生物科技有限公司 | One-piece intermittent immersion plant bioreactor |
| CN104163468A (en) * | 2014-07-29 | 2014-11-26 | 无锡践行中欧科技有限公司 | Adjustable oil slick automatic collecting device and oil slick automatic collecting method |
| CN104511065A (en) * | 2014-12-28 | 2015-04-15 | 陶杰 | Medical infusion device |
-
2017
- 2017-03-21 CN CN201710171154.0A patent/CN106834120A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004089137A (en) * | 2002-09-03 | 2004-03-25 | Olympus Corp | Culture apparatus and method for exchanging culture medium |
| CN203333668U (en) * | 2013-07-04 | 2013-12-11 | 南京博方生物科技有限公司 | One-piece intermittent immersion plant bioreactor |
| CN104163468A (en) * | 2014-07-29 | 2014-11-26 | 无锡践行中欧科技有限公司 | Adjustable oil slick automatic collecting device and oil slick automatic collecting method |
| CN104511065A (en) * | 2014-12-28 | 2015-04-15 | 陶杰 | Medical infusion device |
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Application publication date: 20170613 |