CN106831933A - The method that thick Total saponin is extracted from Chinese yam class plant - Google Patents
The method that thick Total saponin is extracted from Chinese yam class plant Download PDFInfo
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- CN106831933A CN106831933A CN201710046418.XA CN201710046418A CN106831933A CN 106831933 A CN106831933 A CN 106831933A CN 201710046418 A CN201710046418 A CN 201710046418A CN 106831933 A CN106831933 A CN 106831933A
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- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J71/00—Steroids in which the cyclopenta(a)hydrophenanthrene skeleton is condensed with a heterocyclic ring
- C07J71/0005—Oxygen-containing hetero ring
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Abstract
A kind of present invention extracts thick Total saponin method in providing class plant from Chinese yam, comprises the following steps：1）Feedstock treating：The rhizome section or crushing for taking fresh Chinese yam class plant are put into zymotic fluid, are dehydrated, and obtain raw material, and raw material microwave is irradiated, and crush, sieving；2）Extract：Ethanol, cosolvent immersion are added in the raw material crushed, suction filtration obtains filtrate and filter residue, filter residue is irradiated, and adds ethanol immersion, and suction filtration obtains filtrate and filter residue, repeats：Filter residue is irradiated, and adds ethanol immersion, the process of suction filtration 2 ~ 6 times；3）Merge：Filtrate in combining step 2, filtrate is extracted with petroleum ether 3 ~ 6 times repeatedly, stratification, merges petroleum ether layer, and condensing crystallizing obtains thick Total saponin；The present invention carries out separating twice again after being irradiated to the rhizome of the Chinese yam class plant after fermentation discharges cellular content, then is extracted with cosolvent and ethanol, and the thick Total saponin of high content is obtained finally by petroleum ether extraction, and content is more than 96%.
Description
Technical field
The invention belongs to technical field of plant extraction, and in particular to extract the side of thick Total saponin in a kind of class plant from Chinese yam
Method.
Background technology
Dioscoreaceae, winds herbaceous stem or woody climber, and minority is short and small draft, and under ground portion is root-like stock or stem tuber, shape
Various, stem is left-handed or dextrorotation, hairiness or without hair, spinosity or stingless, leaf alternate, sometimes middle part above opposite, and single leaf or palmate are multiple
Leaf, Dan Yechang is heart-shaped or avette, ellipse, and the leaflet of palmately compound leaf is often lanceolar or oval, and base goes out arteries and veins 3-9, lateral vein
It is netted;Petiole is reversed, and base portion has joint, flower unisexuality or both sexes, dioecism, little homophyletic, flower Dan Sheng, fasciation or arrangement sometimes
Into spike, total shape or panicle;The wheel arrangement of male flower tapel (or perianth sliver) 6,2, base portion symphysis or from life;6 pieces of stamen,
Sometimes wherein 3 pieces degenerations, filigree is born on the base portion of perianth or holder, and degeneration ovary is with or without, female flower tapel and male flower
Similar, 3-6 pieces of staminodium or nothing, ovary are the next, Room 3, generally there is ovule 2 per room, and minority category is most, and ovule is born in axis
On placenta, style 3 is separated.Fruit is capsule, berry or samara, and capsule prismatic, every rib aliform, top cracking after maturation is planted
Son has wing or aptery, there is endosperm, and embryo is tiny.
Saponin is the more complicated glycoside compound of a class that there is plant kingdom, and saponin is by sapogenin and sugared two parts group
Into, water can be dissolved in, ethanol, methyl alcohol are dissolved in, it is insoluble in ether or benzene.The abstraction technique of conventional saponin has at present：Use water
Or cold soaking extraction, heating extraction, ultrasonic extraction, the overcritical extraction of aqueous solvent etc..
Such as, Chinese invention patent, the B of Authorization Notice No. CN 102335316 inventions are related to a kind of thick total soap to prior art
A kind of extracting method of glucoside, and in particular to method that thick Total saponin is extracted from fresh Chinese yam class plant.It is a kind of from fresh Chinese yam
The method that thick Total saponin is extracted in class plant, mainly includes the following steps that：Crushing, fermentation, microwave irradiation, separating twice, immersion
Solvent, leach mixed solution, recycling design.Compared with traditional extracting method, the invention is carried out again after employing microwave irradiation
The method of separating twice so that cellular content expands rapidly by microwave irradiation, causes cell membrane fully to expand therewith, then
Separating twice being carried out again, just cell membrane integrally being cracked, cellular content is all discharged, and is fully contacted with solvent, this
Sample also allows for extract sufficiently can be dissolved in solvent, substantially increase the recovery rate of Objective extraction thing, can be reached
More than the 95% of total saponin content in the material, but spontaneous fermentation is taken in the invention, and the slow effect of fermenting speed is less desirable,
Raw material after crushing solute effect in ethanol and recovery rate also have room for promotion.
The content of the invention
A kind of present invention extracts thick Total saponin method in providing class plant from Chinese yam for above-mentioned technical problem, by right
Chinese yam class plant is crushed and ferments and irradiate and is fully dissolved into ethanol cellular content in Chinese yam class plant twice, profit
The recovery rate of dissolution rate and thick Total saponin is improved with cosolvent, extracting method is simple, and thick Total saponin recovery rate is high, operability
By force.
It is of the invention to be for the scheme that above-mentioned technical problem is taken：The side of thick Total saponin is extracted from Chinese yam class plant
Method, comprises the following steps：
1）Feedstock treating：The rhizome section or crushing for taking fresh Chinese yam class plant are put into zymotic fluid, are dehydrated, and raw material are obtained, by raw material
Microwave irradiation, crushes, sieving；
2）Extract：Ethanol, cosolvent immersion are added in the raw material crushed, suction filtration obtains filtrate and filter residue, filter residue is irradiated,
Ethanol immersion is added, suction filtration obtains filtrate and filter residue, repeats：Filter residue is irradiated, and adds ethanol immersion, the process of suction filtration 2 ~ 6 times；
3）Merge：Filtrate in combining step 2, filtrate is extracted with petroleum ether 3 ~ 6 times repeatedly, stratification, merges petroleum ether layer,
Condensing crystallizing obtains thick Total saponin；The present invention carries out separating twice again after being irradiated to the rhizome of the Chinese yam class plant after fermentation to be made
Cellular content is discharged, then is extracted with cosolvent and ethanol, and thick total soap of high content is obtained finally by petroleum ether extraction
Glucoside, content is more than 96%.
Preferably, the Chinese yam class plant in step 1 is：Beautiful leaf Chinese yam, smalt potato, tip fruit Chinese yam, crinosity leaf Chinese yam,
One or more in light leaf Chinese yam, spire D. japonica, hair rattan D. japonica, willow leaf Chinese yam, the root of different Chinese yam class plants
Thick total saponin content is different in stem, and selection can improve the recovery rate of thick Total saponin for one or more.
Preferably, fermentation time is 4 ~ 10h in step 1, prepared by zymotic fluid：By fermented and cultured keynote pH value to 5.2 ~
5.8,4 ~ 9% inoculum concentrations that fermentation medium volume is pressed after sterilizing cooling access lactobacillus acidophilus, training of being fermented under shaking table and normal temperature
Zymotic fluid is supported 2 ~ 3 days to obtain, fermentation medium is made up of following component and weight portion：One or two mixing in urea, dregs of beans
1 ~ 10 part of thing, inorganic ion Mg SO40.2 ~ 2 part, KH2PO41.5 ~ 3.5 parts, ZnSO40.2 ~ 0.6 part, MnCl20.001~
0.003 part, CuSO40.01 ~ 0.03 part, zymotic fluid effectively can extract thick Total saponin, and the rhizome of Chinese yam class plant exists
Irradiated again after fermentation ends and after zymotic fluid is dehydrated together.
Preferably, the rhizome slice thickness of fresh Chinese yam class plant is 0.5 ~ 1cm or is ground into 2 ~ 8 mesh in step 1, profit
In fermentation, ferment effect is lifted, 35 ~ 220 mesh are ground into after raw material microwave is irradiated, sieved, by the rhizome of Chinese yam class plant not
The cell of complete fermentation cracks cell membrane, discharges content.
Preferably, cosolvent is by the weight portion of complexed surfactant 2 ~ 3, the weight portion of mixed solution 2 ~ 3 and distilled water 0.8
~ 1.2 weight portions are constituted, and complexed surfactant is by dihexyl sodium sulfosuccinate, polyoxyethylene nonyl phenolic ether, tetradecylic acid
Isopropyl ester, isodecane, by the amount of material than mol 5: 3：0.2：0.1 proportioning is made, and mixed solution is by analyzing pure AR just
95% ethanol of butanol and the pure AR of analysis, ml is that 3: 4 proportionings are made by volume, and complexed surfactant is mixed with mixed solution
After conjunction, ml is that 4 ~ 6: 1 proportioning is made cosolvent by volume with distilled water, and isopropyl myristate, isodecane are by above-mentioned compound
The contact area of the unexpected technique effect of surfactant remaining composition generation, increase cosolvent and cell, so as to can make
The rhizome cellular content of Chinese yam class plant is fully dissolved into ethanol, and the penetration and compatibilization effect of cosolvent are excellent, hydrotropy
Agent improves dissolution rate and dissolved efficiency and then improves the recovery rate of thick Total saponin.
Preferably, the concentration of alcohol in step 2 is 70% ~ 90%, thick Total saponin is extracted in the ethanol of this range of concentrations
Excellent effect, filter residue soak time in ethanol is 2 ~ 9min, the thick Total saponin in filter residue is fully extracted, it is to avoid material
Material is wasted and can improve the recovery rate and content of thick Total saponin.
Preferably, after removing the moisture of raw material 10 ~ 30% in step 1, being easy to irradiate and crushing, spoke is carried out to it with microwave
According to, and intermittent stirring, when material temperature rises to 60 DEG C to 90 DEG C, cellular content is expanded rapidly by microwave irradiation,
Cause cell membrane fully to expand therewith, crush, cell membrane is integrally cracked, cellular content is all discharged, sieving is improved
Solubility, shortens extraction time.
Compared with prior art, beneficial effects of the present invention are：The root of Chinese yam class plant of the present invention after to fermentation
Stem carries out separating twice again after being irradiated discharges cellular content, then is extracted with cosolvent and ethanol, finally by
Petroleum ether extraction obtains the thick Total saponin of high content, compared with spontaneous fermentation and ethanol that prior art is used are extracted, the present invention
Cellular content in Chinese yam class plant is fully dissolved into ethanol using zymotic fluid and separating twice, recycles cosolvent to improve
The recovery rate of dissolution rate and thick Total saponin, the thick Total saponin of high content is obtained finally by petroleum ether extraction, when the present invention is extracted
Between it is short, impurity content is low, and recovery rate is high, and the thick total saponin content of the extract for obtaining is more than 96%.
Specific embodiment
It is described in further detail with reference to embodiments：
Embodiment 1
The method that thick Total saponin is extracted from Chinese yam class plant, comprises the following steps：
1）Feedstock treating：The rhizome section or crushing for taking fresh Chinese yam class plant are put into zymotic fluid, are dehydrated, and raw material are obtained, by raw material
Microwave irradiation, crushes, sieving；
2）Extract：Ethanol, cosolvent immersion are added in the raw material crushed, suction filtration obtains filtrate and filter residue, filter residue is irradiated,
Ethanol immersion is added, suction filtration obtains filtrate and filter residue, repeats：Filter residue is irradiated, and adds ethanol immersion, the process of suction filtration 2 ~ 6 times；
3）Merge：Filtrate in combining step 2, filtrate is extracted with petroleum ether 3 ~ 6 times repeatedly, stratification, merges petroleum ether layer,
Condensing crystallizing obtains thick Total saponin；The routine techniques such as irradiation, suction filtration, extraction in above-mentioned steps 1 ~ 3 are those skilled in the art institute
In the prior art for knowing, no further details to be given herein, the present invention rhizome of the Chinese yam class plant after fermentation is irradiated after again
Carrying out separating twice discharges cellular content, then is extracted with cosolvent and ethanol, is obtained finally by petroleum ether extraction
The thick Total saponin of high content, content is more than 96%.
Chinese yam class plant in step 1 is：Beautiful leaf Chinese yam, smalt potato, tip fruit Chinese yam, crinosity leaf Chinese yam, light leaf Chinese yam,
One or more in spire D. japonica, hair rattan D. japonica, willow leaf Chinese yam, thick total soap in the rhizome of different Chinese yam class plants
Salidroside content is different, and selection can improve the recovery rate of thick Total saponin for one or more.
Fermentation time is 4 ~ 10h in step 1, prepared by zymotic fluid：By fermented and cultured keynote pH value to 5.2 ~ 5.8, sterilize cold
But 4 ~ 9% inoculum concentrations for pressing fermentation medium volume afterwards access lactobacillus acidophilus, and fermented and cultured is obtained for 2 ~ 3 days under shaking table and normal temperature
Zymotic fluid, fermentation medium is made up of following component and weight portion：1 ~ 10 part of one or two mixtures in urea, dregs of beans,
Inorganic ion Mg SO40.2 ~ 2 part, KH2PO41.5 ~ 3.5 parts, ZnSO40.2 ~ 0.6 part, MnCl20.001 ~ 0.003 part,
CuSO40.01 ~ 0.03 part, zymotic fluid effectively can extract thick Total saponin, and the rhizome of Chinese yam class plant is in fermentation ends
Irradiated again after being dehydrated together with zymotic fluid afterwards.
The rhizome slice thickness of fresh Chinese yam class plant is 0.5 ~ 1cm or is ground into 2 ~ 8 mesh in step 1, beneficial to fermentation, is carried
Ferment effect is risen, 35 ~ 220 mesh are ground into after raw material microwave is irradiated, sieved, by the non-complete fermentation of rhizome of Chinese yam class plant
Cell cracks cell membrane, discharges content.
Cosolvent is by the weight portion of complexed surfactant 2 ~ 3, the weight portion of mixed solution 2 ~ 3 and the weight of distilled water 0.8 ~ 1.2
Part composition, complexed surfactant be by dihexyl sodium sulfosuccinate, polyoxyethylene nonyl phenolic ether, isopropyl myristate,
Isodecane, by the amount of material than mol 5: 3：0.2：0.1 proportioning is made, and mixed solution is by analyzing the n-butanol of pure AR and dividing
95% ethanol of pure AR is analysed, ml is that 3: 4 proportionings are made by volume, after complexed surfactant is mixed with mixed solution, with
Ml is that 4 ~ 6: 1 proportioning is made cosolvent to distilled water by volume, and cosolvent can fill the rhizome cellular content of Chinese yam class plant
Divide and be dissolved into ethanol, the penetration and compatibilization effect of cosolvent are excellent, cosolvent improves dissolution rate and dissolved efficiency and then carries
The recovery rate of thick Total saponin high.
Concentration of alcohol in step 2 is 70% ~ 90%, and the excellent effect of thick Total saponin is extracted in the ethanol of this range of concentrations,
Filter residue soak time in ethanol is 2 ~ 9min, the thick Total saponin in filter residue is fully extracted, it is to avoid waste of material is simultaneously
And the recovery rate and content of thick Total saponin can be improved.
After removing the moisture of raw material 10 ~ 30% in step 1, it is easy to irradiate and crushes, it is irradiated with microwave, and interval
Stirring, when material temperature rises to 60 DEG C to 90 DEG C, makes cellular content be expanded rapidly by microwave irradiation, causes cell membrane
Fully expansion, is crushed therewith, and cell membrane is integrally cracked, and cellular content is all discharged, sieving, improves solubility, contracting
Short extraction time.
Embodiment 2
The method that thick Total saponin is extracted from Chinese yam class plant, comprises the following steps：
1）Feedstock treating：The rhizome section of fresh Chinese yam class plant is taken, thickness is preferably 0.5cm or is ground into preferred 2 mesh and puts
Enter zymotic fluid, fermentation time is preferably 8h, preferably removes 25% moisture, obtains raw material, raw material microwave is irradiated, when on material temperature
Preferred 200 mesh is ground into when rising to preferred 70 DEG C, is sieved；
2）Extract：Ethanol, cosolvent immersion are added in the raw material crushed, concentration of alcohol is preferably 75%, and suction filtration obtains filtrate and filter
Slag, filter residue is irradiated, and adds ethanol immersion, and concentration of alcohol is preferably 75%, and suction filtration obtains filtrate and filter residue, preferably repeats：Filter
Slag is irradiated, and adds ethanol immersion, the process of suction filtration 4 times；
3）Merge：Filtrate in combining step 2, preferably extracts filtrate 4 times repeatedly with the petroleum ether of suction filtration liquid 1/3 ~ 1/2, excellent
The petroleum ether of suction filtration liquid 1/5, stratification is selected to merge petroleum ether layer, condensing crystallizing obtains thick Total saponin.
The existing skill of the routine techniques such as irradiation, suction filtration, extraction in above-mentioned steps 1 ~ 3 dawn known to those skilled in the art
Art, no further details to be given herein, and the present invention carries out separating twice again after being irradiated to the rhizome of the Chinese yam class plant after fermentation
Cellular content is discharged, then is extracted with cosolvent and ethanol, the thick total of high content is obtained finally by petroleum ether extraction
Saponin, content is more than 96%.
Chinese yam class plant in step 1 is：Beautiful leaf Chinese yam, smalt potato, tip fruit Chinese yam, crinosity leaf Chinese yam, light leaf Chinese yam,
One or more in spire D. japonica, hair rattan D. japonica, willow leaf Chinese yam.
In step 1, prepared by zymotic fluid：By fermented and cultured keynote pH value to preferred 5.6, fermented and cultured is pressed after sterilizing cooling
Matrix accumulates preferred 5% inoculum concentration and accesses lactobacillus acidophilus, and fermented and cultured obtains zymotic fluid in preferred 2 days under shaking table and normal temperature, shakes
The rotating speed of bed is preferably 200r/min, and cultivation temperature is preferably 30 DEG C, and fermentation medium is by following component and preferred weight part group
Into：6 parts of one or two mixtures, inorganic ion Mg SO in urea, dregs of beans40.8 part, KH2PO42 parts, ZnSO40.4
Part, MnCl20.002 part, CuSO40.02 part, zymotic fluid effectively can extract thick Total saponin, the rhizome of Chinese yam class plant
Irradiated again after fermentation ends and after zymotic fluid is dehydrated together.
Cosolvent is by the weight portion of preferred complexed surfactant 2.6, the weight portion of mixed solution 2.5 and the weight of distilled water 1
Part composition, complexed surfactant be by dihexyl sodium sulfosuccinate, polyoxyethylene nonyl phenolic ether, isopropyl myristate,
Isodecane, by the amount of material than mol 5: 3：0.2：0.1 proportioning is made, and mixed solution is by analyzing the n-butanol of pure AR and dividing
95% ethanol of pure AR is analysed, ml is that 3: 4 proportionings are made by volume, after complexed surfactant is mixed with mixed solution, with
Distilled water by volume ml be preferably 5: 1 proportioning be made cosolvent, cosolvent can make the rhizome cellular content of Chinese yam class plant
Fully be dissolved into ethanol, the penetration and compatibilization effect of cosolvent are excellent, cosolvent improve dissolution rate and dissolved efficiency and then
Improve the recovery rate of thick Total saponin.
After complexed surfactant mixes with mixed solution, ml is not limited only to 4 ~ 6: 1 proportioning by volume with distilled water
Cosolvent is made, should also include 4：1、4.1：1、4.2：1、4.3：1……5.9：1、6：1.
Embodiment of above is merely to illustrate the present invention, and not limitation of the present invention, the ordinary skill people of this area
Member, without departing from the spirit and scope of the present invention, can also make a variety of changes and modification.Therefore, it is all equivalent
Technical scheme fall within scope of the invention, scope of patent protection of the invention should be defined by the claims.
Claims (7)
1. the method that thick Total saponin is extracted from Chinese yam class plant, it is characterised in that：The extracting method is comprised the following steps：
1）Feedstock treating：The rhizome section or crushing for taking fresh Chinese yam class plant are put into zymotic fluid, are dehydrated, and raw material are obtained, by raw material
Microwave irradiation, crushes, sieving；
2）Extract：Ethanol, cosolvent immersion are added in the raw material crushed, suction filtration obtains filtrate and filter residue, filter residue is irradiated,
Ethanol immersion is added, suction filtration obtains filtrate and filter residue, repeats：Filter residue is irradiated, and adds ethanol immersion, the process of suction filtration 2 ~ 6 times；
3）Merge：Filtrate in combining step 2, filtrate is extracted with petroleum ether 3 ~ 6 times repeatedly, stratification, merges petroleum ether layer,
Condensing crystallizing obtains thick Total saponin.
2. the method that thick Total saponin is extracted in the class plant from Chinese yam according to claim 1, it is characterised in that：The step
Chinese yam class plant in 1 is：Beautiful leaf Chinese yam, smalt potato, tip fruit Chinese yam, crinosity leaf Chinese yam, light leaf Chinese yam, spire Japan potato
One or more in Chinese yam, hair rattan D. japonica, willow leaf Chinese yam.
3. the method that thick Total saponin is extracted in the class plant from Chinese yam according to claim 1, it is characterised in that：The step
Fermentation time is 4 ~ 10h in 1, prepared by zymotic fluid：By fermented and cultured keynote pH value to 5.2 ~ 5.8, trained by fermentation after sterilizing cooling
4 ~ 9% inoculum concentrations for supporting matrix product access lactobacillus acidophilus, and fermented and cultured obtains zymotic fluid, the hair in 2 ~ 3 days under shaking table and normal temperature
Ferment culture medium is made up of following component and weight portion：1 ~ 10 part of one or two mixtures in urea, dregs of beans, inorganic ion
Mg SO40.2 ~ 2 part, KH2PO41.5 ~ 3.5 parts, ZnSO40.2 ~ 0.6 part, MnCl20.001 ~ 0.003 part, CuSO40.01~0.03
Part.
4. the method that thick Total saponin is extracted in the class plant from Chinese yam according to claim 1, it is characterised in that：The step
The rhizome slice thickness of fresh Chinese yam class plant is 0.5 ~ 1cm or is ground into 2 ~ 8 mesh in 1, is ground into after raw material microwave is irradiated
35 ~ 220 mesh, sieving.
5. the method that thick Total saponin is extracted in the class plant from Chinese yam according to claim 1, it is characterised in that：The hydrotropy
Agent is made up of the weight portion of complexed surfactant 2 ~ 3, the weight portion of mixed solution 2 ~ 3 and the weight portion of distilled water 0.8 ~ 1.2, described multiple
It is by dihexyl sodium sulfosuccinate, polyoxyethylene nonyl phenolic ether, biochemical hyaluronic acid, isodecane, by thing to close surfactant
The amount of matter is than mol 5: 3：0.2：0.1 proportioning is made, and the mixed solution is the n-butanol and the pure AR of analysis by analyzing pure AR
95% ethanol, by volume ml be 3: 4 proportioning be made, after complexed surfactant is mixed with mixed solution, pressed with distilled water
Volume ratio ml is that 4 ~ 6: 1 proportioning is made cosolvent.
6. the method that thick Total saponin is extracted in the class plant from Chinese yam according to claim 1, it is characterised in that：The step
Concentration of alcohol in 2 is 70% ~ 90%, and filter residue soak time in ethanol is 2 ~ 9min.
7. the method that thick Total saponin is extracted in the class plant from Chinese yam according to claim 1, it is characterised in that：The step
After removing the moisture of raw material 10 ~ 30% in 1, it is irradiated with microwave, and intermittent stirring, when material temperature rises to 60 DEG C extremely
At 90 DEG C, crush, sieving.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111116700A (en) * | 2019-11-25 | 2020-05-08 | 华南农业大学 | Method for extracting, separating and purifying dioscin from chrysanthemum leaves |
| CN112021441A (en) * | 2020-08-21 | 2020-12-04 | 湖南汇富康达健康管理研究院 | Low-GI (glycemic index) full-nutrition bar and preparation method thereof |
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| CN103497987A (en) * | 2013-09-16 | 2014-01-08 | 陕西科技大学 | Clean production method of yam diosgenin |
| CN103882086A (en) * | 2013-12-28 | 2014-06-25 | 赵长伟 | Extraction method of dioscorea composita saponin |
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| CN101485814A (en) * | 2009-02-16 | 2009-07-22 | 李万忠 | Supercritical CO2 extraction method for saponin component in Paris polyphylla var. yunnanensis |
| CN102335316A (en) * | 2010-12-14 | 2012-02-01 | 万绍平 | Extraction method of raw total saponins from fresh dioscorea plants |
| CN102432665A (en) * | 2011-09-27 | 2012-05-02 | 陕西科技大学 | Alcohol-free extraction method for dioscin |
| CN103497987A (en) * | 2013-09-16 | 2014-01-08 | 陕西科技大学 | Clean production method of yam diosgenin |
| CN103882086A (en) * | 2013-12-28 | 2014-06-25 | 赵长伟 | Extraction method of dioscorea composita saponin |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111116700A (en) * | 2019-11-25 | 2020-05-08 | 华南农业大学 | Method for extracting, separating and purifying dioscin from chrysanthemum leaves |
| CN111116700B (en) * | 2019-11-25 | 2021-08-03 | 华南农业大学 | Method for extracting, separating and purifying dioscin from chrysanthemum leaves |
| CN112021441A (en) * | 2020-08-21 | 2020-12-04 | 湖南汇富康达健康管理研究院 | Low-GI (glycemic index) full-nutrition bar and preparation method thereof |
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