CN106823475B

CN106823475B - Blood shunt

Info

Publication number: CN106823475B
Application number: CN201710066327.2A
Authority: CN
Inventors: 徐文峰; 廖晓玲
Original assignee: Chongqing University of Science and Technology
Current assignee: BEIJING JORFERIN BIO-TECHNOLOGY CO.,LTD.
Priority date: 2017-02-07
Filing date: 2017-02-07
Publication date: 2023-01-24
Anticipated expiration: 2037-02-07
Also published as: CN106823475A

Abstract

The invention provides a blood shunt which mainly comprises a main body and a fluid shunt channel. The method is characterized in that: the main body is disc-shaped, and fluid shunting channels which are spiral, closed up and down and unequal in width are processed from the edge of the disc to the center of a circle. The fluid diversion channel of the main body is more than or equal to 3 layers. A section of drainage dike is processed on a channel central line at the beginning of each layer of the diversion channel, diversion island walls with the number more than or equal to 3 are processed at the downstream of the drainage dike, the diversion channel is divided into an inner flow channel and an outer flow channel from the diversion port by the diversion island walls, a convex rotary collision wall is processed at each inner flow channel, the downstream of the rotary collision wall is next to a separation port, and the separation port is arranged between the two diversion island walls and communicates the inner flow channel with the outer flow channel. The cell separation device has the advantages that the cell separation device can be used for rapidly separating cells, is simple and convenient to operate and is not easy to cause cell damage, liquid can be fed into the cell separation device while the cell separation device is used for separating cells, the cells can be separated repeatedly and circularly, and the separation efficiency is improved.

Description

Blood shunt

Technical Field

The invention relates to a blood separating device, in particular to a blood shunt which is suitable for separating blood cells in common medical institutions.

Technical Field

At present, there are two technical methods for separating blood cells, namely, membrane separation technology and centrifugal separation technology. The membrane separation technology is to use a membrane with selective permeability as a separation medium, a stock solution passes through one side of the membrane under a certain pressure, a solvent and a small molecular solute permeate through the membrane wall to be a permeate, and a larger molecular solute is intercepted by a membrane, so that the purposes of substance separation and concentration are achieved. The membrane separation technology adopts non-cross flow filtration, so that the membrane is easy to block, and adopts cross flow filtration, although the membrane is not easy to block, the requirement on the performance of the membrane is high, and the cost is increased. The centrifugal separation technology is that blood components are separated from each other according to specific gravity difference through a centrifugal machine separation device so as to obtain required blood components, but the cells are easy to be mechanically damaged and polluted, and the cells have phase change, so that the cell quality is influenced; meanwhile, the environmental requirements on the laboratory are very strict, and the cost of cell separation is increased.

Therefore, the invention solves the problem and provides a technical scheme of a simple and efficient blood shunt which has the advantages of minimum cell loss, no cell phase change and convenience for subsequent analysis.

Disclosure of Invention

In order to solve the problems, the technical scheme of the invention is as follows: a blood shunt mainly comprises a main body and a fluid shunting channel. The method is characterized in that: the main body is disc-shaped, and spiral fluid shunting channels which are closed up and down and have unequal widths are processed from the edge of the disc to the center of a circle. The fluid diversion channel of the main body is more than or equal to 3 layers. The first layer of flow dividing channels start from a total sample inlet processed at the edge of the disc and end at an outlet of the first layer of outer flow channel. A section of drainage dike is processed on the channel central line at the beginning of the shunting channel of each layer; the downstream of the drainage dike is provided with more than or equal to 3 diversion island walls, and the diversion island walls divide the diversion channel into an inner flow channel and an outer flow channel from the diversion port; a convex rotary collision wall is processed at each inner flow channel, and a sorting opening is next to the downstream of the rotary collision wall; the separation port is arranged between the two flow-dividing island walls and is used for communicating the inner flow channel with the outer flow channel. The 1 rotary collision wall and the 2 diversion island walls form a sorting unit. The inlet of the inner flow passage beside the sorting port becomes the flow dividing port of the next sorting unit. The design is actually that the sorting unit is taken as a unit, and the layered sorting is repeated. The second layer of flow dividing channels start from the flow guiding embankment of the second layer and end at the outlet of the outer flow channel of the second layer. The third layer of flow dividing channels start from the drainage dike of the third layer and end at the outlet of the outer flow passage of the third layer. And (4) analogizing the flow diversion channels of the following layers in sequence, and finally finishing the total sample outlet of the inner flow channel at the center of the circle of the main body.

In the technical scheme, the drainage dike is a strip with the width of 0.5mm to 1mm and the height dimension of 2mm to 5mm lower than the top of the fluid diversion channel. The width of each layer of fluid diversion channel is consistent, the width size is more than or equal to 5mm, or the width of each layer of fluid diversion channel increases layer by layer or decreases layer by layer. The diversion island wall is in a fish shape, a wave shape and a diamond shape along the flowing direction of the fluid diversion channel, and the design ensures that the arc-shaped direction of the inner flow channel of one sorting unit is different from the front section and the rear section of the channel; or in the shape of a fan or a horn, and the design ensures that the arc direction of the inner flow passage of one sorting unit is consistent between the front section and the rear section of the passage. The height of the flow dividing island wall is flush with the top of the fluid flow dividing channel, namely the fluid flow dividing channel is closed up and down.

In the technical scheme, the outer flow channel is an arc-shaped channel with smooth and smooth side wall surfaces and stable transition along the same direction of the main body arc; the width of the outer flow channels of each layer is consistent, the width dimension is less than or equal to 1/4 of the width dimension of the fluid diversion channel, or the width dimension of the outer flow channels of each layer is gradually reduced or gradually increased.

In the technical scheme, the width sizes of the front section channel, the middle section channel and the rear section channel of the inner flow channel in one sorting unit are different, and the width sizes of the inner flow channels of all layers are different. The shape of the inner flow passage: the front half section of one side of the inner runner of the diversion island wall is in an arc shape with a reverse main body arc radian, and the rear half section of the convex surface of the corresponding rotary collision wall is in an arc shape with a same main body arc radian; and the convex part of the rotary collision wall is in the arc radian direction of the reverse main body. The diversion island wall and the rotary collision wall are correspondingly matched, the front half section of the inner flow channel is an arc rotary curve with a reverse main body arc radian, and the front half section of the inner flow channel passes through the rotary collision wall and then is a channel with a same main body arc radian. Or the shape of the inner flow passage: the diversion island wall and the rotary collision wall are in the direction of the arc radian of the reverse main body at one side of the inner flow channel, so that the inner flow channel is a channel with the arc radian of the reverse main body.

In the technical scheme, the diameters of the total sample inlet and the total sample outlet are consistent and are larger than the outlet diameters of the outer runners of all the layers. The design ensures that the inner flow passage has enough flow velocity. The outlet diameter of each layer of outer flow passage needs to be measured and calculated by using fluid simulation software according to the overall designed shape and size. The height dimension of the bottom of the outer runner is 0.5mm to 2mm higher than the height of the bottom of the inner runner. The bottom of the outer runner and the bottom of the inner runner are arranged at a sorting port and are connected and transited through the bottom of the sorting port with an arc bulge; the bottom of the outer runner is connected and transited to form a horizontal bottom surface, or connected and transited to form an inclined bottom surface with a high transition part and a low outer side.

In the technical scheme, one end of the diversion island wall surface opposite to the direction of the fluid is a tapered diversion island wall tip. The conical tip of the wall tip of the diversion island is sharpened into an end face with the width size being more than or equal to 1mm, and a convex irregular convex face is machined on the end face from one side of the inner flow passage; the area of the irregular convex surface gradually diverges to one side of the outer flow passage from top to bottom and is reduced, and the height of the irregular convex surface gradually decreases and is finally integrated with the irregular concave surface in the middle of the fork diverging from top to bottom of the irregular convex surface. Irregular convex surfaces and irregular concave surfaces which are different in size and are unevenly distributed are machined on the side wall surface of the rotary collision wall surface opposite to the fluid direction. The design is to increase the fluid convolution, increase the fluid mixed flow and the vortex, and reduce the laminar flow phenomenon in the micro-channel. To facilitate separation of blood components and cells.

In the technical scheme, a dark barrier dam can be processed at the diversion port of the sorting unit of the main body; the dark dam is a dam wall which is transversely penetrated and intercepted in the inner flow channel and is lower than the top of the inner flow channel by the height dimension of 2mm-5mm. This design is to increase the initial velocity of the fluid entering the inner channel. A separation port hidden dike can be processed at the separation port of the flow dividing island wall of the main body; the hidden dike of the sorting port is a dike wall which is formed by processing a protruding section at one end of the diversion island wall opposite to the fluid and in the same direction with the fluid and transversely intercepts the sorting port, but does not completely penetrate through the sorting port, and the height dimension of the hidden dike is lower than the top of the sorting port by 2mm to 5mm. The design is to increase the separation efficiency of the separation port and further control the separation size.

The invention applies the 'flying sand' separation principle of Dujiang weir water conservancy, namely, the theory of the swirling flow and the centrifugal force action, and applies the principle to the design of blood separation. At present, a great deal of research on blood separation of microchannel chips is carried out at home and abroad, but the microchannels are mostly laminar flows, and the conventional fluid theory is not applicable. The invention combines the conventional water conservancy fluid and the blood fluid and applies the combination to the separation of blood components and cells in non-micron channels. And separating out large-mass protein and cells at a sorting port. The separation of blood cells is achieved by circulating and sorting for a plurality of times.

The invention aims to provide a cell separation device which can rapidly separate cells, is simple and convenient to operate and is not easy to cause cell damage. The dispenser makes the operation of cell separation and collection simple and easy.

Drawings

FIG. 1 is a schematic top view of the present invention.

Fig. 2 is a schematic view of a sorting unit of the present invention.

Fig. 3 is a schematic view of the end face a of the diversion island tip of the present invention.

Fig. 4 is a schematic cross-sectional view D-D of the end face a of the diversion island wall tip of the present invention.

Fig. 5 is a schematic view of the end face B of the convolute impact wall of the present invention.

Fig. 6 is a schematic cross-sectional view of a drainage bank of the present invention.

Fig. 7 is a schematic view of three sorting units of a sector-shaped diversion island wall according to the present invention.

FIG. 8 isbase:Sub>A schematic cross-sectional view ofbase:Sub>A sorting port A-A at the bottom horizontal bottom surface of an external flow channel according to the present invention.

FIG. 9 isbase:Sub>A schematic sectional view ofbase:Sub>A sorting port A-A of the inclined bottom surface of the bottom of the outer flow path according to the present invention.

Fig. 10 is a schematic view of three sorting units with dark banks according to the present invention.

Fig. 11 is a schematic B-B sectional view of a dark barrier bank of the present invention.

FIG. 12 is a schematic C-C cross-sectional view of a sorting port dark bank of the present invention.

In the figure: 1. a total sample inlet; 2. a first layer of flow-splitting channels; 3. a drainage dike; 4. a shunt port; 5. a diversion island wall; 6. an inner flow passage; 7. an outer flow passage; 8. a sorting port; 9. revolving and bumping the wall; 10. a total sample outlet; 11. a main body; 12. a third layer of flow distribution channels; 13. an outlet of the third layer of outer flow channel; 14. an outlet of the second outer runner; 15. a second layer of flow dividing channels; 16. an outlet of the first layer of outer flow channel; 17. a turning round; 18. a dark barrier dam; 19. a hidden dike at the sorting port; 20. shunting island wall tips; 21. an irregular convex surface; 22. an irregular concave surface; 23. an inner runner bottom; 24. the bottom of the sorting port; 25. the bottom of the outer runner.

Detailed Description

The invention is further described below with reference to the figures and examples.

Example one

Referring to the shape structures of fig. 1 to 9, in a blood flow splitter, the number of flow splitting channels of a main body 11 is 3, and 9 splitting island walls 5 in each layer form 8 sorting units, that is, 8 sorting ports 8.

Each fluid diversion channel has a depth of 8mm and a width of 10mm. The width of the drainage dike 3 is 1mm, and the height thereof is 6mm. The diversion island wall 5 is fish-shaped along the direction of the fluid diversion channel, and the height of the diversion island wall is 8mm and is flush with the top of the fluid diversion channel; the end face of the diversion island tip 20 is 2mm wide. The width of the outer flow channel 7 of each layer is 2.5mm; the height dimension of the outer flow passage bottom 25 of each layer is 2mm higher than that of the inner flow passage bottom 23; the outer runner bottom 25 is connected and transited to form a horizontal bottom surface. The two ends of the inner flow passage 6 of each layer are opened by a horn, the front half section of the inner flow passage is an arc-shaped rotary curve 17 with a reverse main body 11 arc radian, and the width of the end of the rotary curve 17 is narrowest; the inner flow passage 6 is a passage with the same direction of the arc radian of the main body 11 after passing through the rotary collision wall 9. The diameters of the total sample inlet 1 and the total sample outlet 10 are consistent to be 9812 mm, and the diameters of the first layer outer runner outlet 16, the second layer outer runner outlet 14 and the third layer outer runner outlet 13 are 9810.9 mm.

Example two

Referring to the shape structure of fig. 1 to 12, a blood flow splitter, the number of flow splitting channels of a main body 11 is 5, and each layer of 6 splitting island walls 5 constitutes 5 sorting units, i.e., 5 sorting ports 8.

The depth of the first layer of fluid diversion channel is 10mm, and the width of the first layer of fluid diversion channel is 12mm; the depth of the second fluid diversion channel is 10mm, and the width of the second fluid diversion channel is 11mm; the third layer of fluid diversion channel has the depth of 10mm and the width of 10mm; the depth of the fourth fluid diversion channel is 10mm, and the width of the fourth fluid diversion channel is 9mm; the fifth layer of fluid diversion channels has a depth of 10mm and a width of 8mm. The width of the drainage dike 3 is 0.5mm, and the height thereof is 5mm. The flow dividing island wall 5 is in a fan shape along the flow dividing channel, and the height of the flow dividing island wall is 10mm and is flush with the top of the flow dividing channel; the end face of the diversion island tip 20 is 1mm wide. The width of the first layer of outer flow channel is 7 mm; the width of the second layer outer runner 7 is 2.5mm; the width of the third layer of outer flow channel 7 is 2mm; the width of the fourth layer external flow channel 7 is 1.5mm; the fifth-layer outer flow passage 7 is 1.5mm wide. The height dimension of the outer flow passage bottom 25 of each layer is 1mm higher than that of the inner flow passage bottom 23; the outer flow channel bottom 25 is connected with an inclined bottom surface which is high at the transition part and low at the outer side after transition. The two ends of the inner flow passage 6 of each layer are opened by a horn, and are arc-shaped rotary curves 17 with the arc radian of the reverse main body 11, and the width of the end of the rotary curve 17 is narrowest. The diameters of the total sample inlet 1 and the total sample outlet 10 are consistent to be 9813 mm, and the diameters of the first layer to the fifth layer outer flow passage outlet are all 9811.2 mm.

And dark barrier dikes 18 with the height dimension being 5mm lower than the top of the inner flow channel 6 are processed at the branch ports 4 of the third-layer sorting unit and the fifth-layer sorting unit. And sorting port hidden dikes 19 with the height dimension 2mm lower than the tops of the sorting ports 8 are processed at the sorting ports 8 of the third-layer and fifth-layer diversion island walls 5.

EXAMPLE III

Referring to the shape structures of fig. 1 to 12, a blood flow diverter has a main body 11 with flow diversion channels =4 layers, and 7 diversion island walls 5 in each layer form 6 sorting units, i.e., 6 sorting ports 8.

The depth of the first layer of fluid diversion channel is 10mm, and the width of the first layer of fluid diversion channel is 9mm; the depth of the second fluid diversion channel is 10mm, and the width of the second fluid diversion channel is 10mm; the depth of the third layer of fluid flow distribution channel is 10mm, and the width of the third layer of fluid flow distribution channel is 11mm; the fourth fluid diversion channel is 10mm deep and 12mm wide. The width of the drainage dike 3 is 0.5mm, and the height thereof is 7mm. The flow dividing island wall 5 is fish-shaped along the flow dividing channel, and the height of the flow dividing island wall is 10mm and is flush with the top of the flow dividing channel; the end face of the diversion island tip 20 is 1mm wide. The width of the first layer of outer flow channel 7 is 1.5mm; the width of the second layer external flow channel 7 is 2mm; the width of the third layer of outer flow channel 7 is 2.5mm; the width of the fourth layer external flow channel 7 is 3mm. The height dimension of the outer flow passage bottom 25 of each layer is 1.5mm higher than that of the inner flow passage bottom 23; the outer flow channel bottom 25 is connected with an inclined bottom surface which is high at the transition part and low at the outer side after transition. The two ends of the inner flow channel 6 of each layer are opened by a horn, the inner flow channel is an arc-shaped rotary bend 17 with the arc radian of the reverse main body 11, and the width of the end of the rotary bend 17 is narrowest; the inner flow passage 6 is a passage with the same direction of the arc radian of the main body 11 after passing through the rotary collision wall 9. The diameters of the total sample inlet 1 and the total sample outlet 10 are consistent to be \9812.5 mm, and the diameters of the first layer to the fifth layer outer runner outlet are all \9811mm.

The diversion ports 4 of the sorting units in each layer are processed with dark dam 18 with a height dimension 3mm lower than the top of the inner flow channel 6. Sorting opening hidden dikes 19 with the height dimension being 3mm lower than the tops of the sorting openings 8 are processed at the sorting openings 8 of the diversion island walls 5 of each layer.

Claims

1. A blood shunt mainly comprises a main body (11) and a fluid shunting channel, and is characterized in that: the main body (11) is disc-shaped, and fluid shunting channels which are spiral, closed up and down and unequal in width are processed from the edge of the disc to the circle center; the fluid flow-dividing channel of the main body (11) is more than or equal to 3 layers; the first layer of shunting channels (2) start from a total sample inlet (1) processed at the edge of the disc and end at a first layer of outer flow channel outlet (16); a section of drainage dike (3) is processed on the channel central line at the beginning of the shunting channel of each layer; the downstream of the drainage dike (3) is provided with more than or equal to 3 diversion island walls (5), and the diversion island walls (5) divide the diversion channel into an inner runner (6) and an outer runner (7) from the diversion port (4); a convex rotary collision wall (9) is processed at each inner flow channel (6), and a sorting opening (8) is closely arranged at the downstream of the rotary collision wall (9); the separation port (8) is arranged between the two diversion island walls (5) and is used for communicating the inner runner (6) with the outer runner (7); the 1 rotary collision wall (9) and the 2 diversion island walls (5) form a separation unit; the inlet of the inner flow channel (6) beside the sorting port (8) becomes the flow dividing port (4) of the next sorting unit; the second layer of flow dividing channels (15) start from the drainage dike (3) of the second layer and end at the outlet (14) of the outer flow channel of the second layer; the third layer of flow dividing channels (12) start from the drainage dike (3) of the third layer and end at the outlet (13) of the outer flow passage of the third layer; the fluid diversion channels of the following layers are analogized in sequence, and finally, the inner flow passage (6) is ended at a main sample outlet (10) at the center of the circle of the main body (11);

the diversion island wall (5) is in a fish shape, a wave shape and a diamond shape along the trend of the fluid diversion channel; one end, facing the fluid, of the diversion island wall (5) opposite to the direction of the fluid is a tapered diversion island wall tip (20), the tapered tip of the diversion island wall tip (20) is sharpened into an end face with the width dimension being more than or equal to 1mm, and a convex irregular convex face (21) is machined on the end face from one side of the inner flow channel (6); the area of the irregular convex surface (21) gradually decreases towards the upper and lower branches of one side of the outer flow passage (7), the height is also gradually reduced, and finally the irregular convex surface and the irregular concave surface (22) in the middle of the upper and lower branches of the irregular convex surface (21) are integrated; the side wall surface of the rotary collision wall (9) facing the fluid and opposite to the direction of the fluid is provided with irregular convex surfaces (21) and irregular concave surfaces (22) which are different in size and are distributed unevenly;

the front half section of one side of the inner runner (6) of the flow dividing island wall (5) is in an arc shape with a reverse main body arc radian, so that the shape of the inner runner (6) is in an arc shape with a same main body (11) arc radian at the back half section corresponding to the convex surface of the rotary collision wall (9); the convex part of the rotary collision wall (9) is in the direction of the arc radian of the reverse main body, the diversion island wall (5) is correspondingly matched with the rotary collision wall (9), the front half section of the inner flow channel (6) is an arc rotary curve with the arc radian of the reverse main body, and the front half section of the inner flow channel passes through the rotary collision wall (9) and then is a channel with the arc radian of the same direction main body (11).

2. A blood shunt according to claim 1, wherein: the drainage dike (3) is a strip with the width of 0.5mm to 1mm and the height dimension of 2mm to 5mm lower than the top of the fluid diversion channel; the width of each layer of fluid diversion channel is consistent, the width size is more than or equal to 5mm, or the width of each layer of fluid diversion channel is increased layer by layer or reduced layer by layer; the height of the flow dividing island wall (5) is flush with the top of the fluid flow dividing channel, namely the fluid flow dividing channel is closed up and down.

3. A blood shunt according to claim 1, wherein: the outer flow passage (7) is an arc-shaped passage with smooth side wall surfaces and smooth and stable transition along the same direction of the arc of the main body (11); the width of the outer flow passages (7) of each layer is consistent, the width dimension is less than or equal to 1/4 of the width dimension of the fluid diversion passage, or the width dimension of the outer flow passages (7) of each layer is gradually reduced or gradually increased.

4. A blood shunt according to claim 1, wherein: the width sizes of the front section channel, the middle section channel and the rear section channel of the inner flow channel (6) in one sorting unit are different, and the width sizes of the inner flow channels (6) in each layer are different.

5. A blood shunt according to claim 1, wherein: the diameters of the total sample inlet (1) and the total sample outlet (10) are consistent and are larger than the outlet diameter of each layer of outer flow channel (7); the height dimension of the outer flow channel bottom (25) of the outer flow channel (7) is 0.5mm to 2mm higher than the height of the inner flow channel bottom (23) of the inner flow channel (6); the outer flow channel bottom (25) and the inner flow channel bottom (23) are connected and transited at the separation port (8) through the separation port bottom (24) with an arc bulge; the bottom (25) of the outer runner is connected and transited to form a horizontal bottom surface, or is connected and transited to form an inclined bottom surface with a high transition part and a low outer side.

6. A blood shunt according to claim 1, wherein: the main body (11) can be provided with a dark barrier dike (18) at the position of a flow-dividing port (4) of the sorting unit; the dark dam (18) is a dam wall which is transversely penetrated and intercepted in the inner flow channel (6) and has the height dimension of being lower than the top of the inner flow channel (6) by 2mm to 5mm; a sorting opening hidden dike (19) can be processed on the sorting opening (8) of the flow dividing island wall (5) of the main body (11); the sorting opening hidden dike (19) is a section of convex dike wall processed at one end of the diversion island wall (5) opposite to the fluid and in the same direction with the fluid, transversely intercepted at the sorting opening (8) and not completely communicated with the sorting opening (8); the height dimension of the sorting opening hidden dike (19) is lower than the top of the sorting opening (8) by 2mm-5mm.