CN106821979A - A kind of new medical antimicrobial spray - Google Patents
A kind of new medical antimicrobial spray Download PDFInfo
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- CN106821979A CN106821979A CN201710027863.1A CN201710027863A CN106821979A CN 106821979 A CN106821979 A CN 106821979A CN 201710027863 A CN201710027863 A CN 201710027863A CN 106821979 A CN106821979 A CN 106821979A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/12—Aerosols; Foams
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
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Abstract
The present invention relates to a kind of new medical antimicrobial spray and preparation method thereof.New medical antimicrobial spray prepared by this method is made up of oxidation of polysaccharides, disodium hydrogen phosphate, glycerine and sterilized water, and wherein the mass ratio of oxidation of polysaccharides, disodium hydrogen phosphate, glycerine and sterilized water is (1 ~ 20)：(10~20)：20：500.Present invention selection raw material sources are extensive, hydrophily is preferable, cytotoxicity is low, biocompatibility is good and biodegradable oxidation of polysaccharides is additive, and its excellent broad spectrum antibacterial performance is realized by the aldehyde radical and carboxyl that contain in oxidation of polysaccharides molecule.The new medical antimicrobial spray that the present invention is provided, its preparation method is simple and easy to apply, is suitable to large-scale production.Therefore, the new medical antimicrobial spray that the present invention is provided has good market application foreground in pharmaceuticals industry.
Description
Technical field
The present invention relates to a kind of spray and its preparation technology, and in particular to a kind of oxidation of polysaccharides with broad-spectrum antibacterial resists
Bacterium spray and its production technology.
Background technology
The wound that people are easily caused by external environment condition in daily life, more serious wound needs operation stitching
Or the attaching wound dressing of large area carries out the healing of wound.When wound area is less, usually used medicine is soft
The paste such as cream, but this drug absorption is poor, and drug effect is difficult to play, and wound is susceptible to infection.With alcohol, Iodophor etc.
During reason wound, there is larger excitant to wound, produce violent pain.Therefore needing one kind can quickly kill to wound surface
Bacterium is processed and bland wound spray.
The spray species for surface of a wound antibacterial for occurring in the market is fewer, and major part is silver ion spray, shell gathers
The antimicrobial spray of sugared spray or alginate.The use of wherein silver ion spray is easily caused wound and darkens, while European Union
Associated mechanisms to the harm that environment is produced using silver ion to illustrating worry.The spray of shitosan spray and alginate
Antibacterial effect depend on the antimicrobial component of wherein extra addition so that antibacterial effect is limited, and with the increase of resting period,
The loss of antimicrobial component, causes the stability of product to decline.
The addition of natural polysaccharide, majority is for improving the stabilization of the film forming and antimicrobial component of spray in spray solution
Property, and natural polysaccharide HMW in itself, high viscosity and low solubility significantly limit its use in spray.Polysaccharide
Oxidized modified, the aldehyde radical on its molecule is significantly increased with carboxyl-content so that the hydrophily of oxidation of polysaccharides increases, so that
Its solubility in aqueous is improved with stability, thus has significantly widened application of the oxidation of polysaccharides in spray field.Oxidation
The content of aldehyde radical is substantially increased in polysaccharide molecule, and the material for containing a large amount of aldehyde radicals can be with the cell membrane phase interaction of bacterium
With, the cell membrane of bacterium is destroyed, so as to reach the effect of sterilization, therefore polysaccharide is oxidized rear with very strong bactericidal property.Oxygen
Change the ratio of aldehyde radical and carboxyl-content in polysaccharide molecule, have impact on the antibacterial effect of oxidation of polysaccharides to a certain extent, therefore
Oxidation of polysaccharides is selected according to the content requirement of certain aldehyde radical carboxyl in practical application.
The present invention is from the oxidized rear oxidation of polysaccharides with certain aldehyde radical and carboxyl-content as medical antibacterial spray
Antimicrobial component, without other antiseptics and film forming agent is additionally added, preparation process is simple, Costco Wholesale is cheap.
The content of the invention
It is an object of the invention to provide a kind of new medical spray with anti-microbial property and preparation method thereof, especially one
Plant natural, environmental protection, the preparation method of the new medical spray with antibacterial action.
Technical solution of the invention is：A kind of new medical antimicrobial spray, it is characterised in that by oxidation of polysaccharides, phosphoric acid
Disodium hydrogen, glycerine and sterilized water composition, its pH scope is between 5.4 ~ 7.8, and its preparation method is as follows：
(1) preparation of hydroformylation polysaccharide：In mass ratio 1:Be dissolved in natural polysaccharide in deionized water and obtain natural many by (10 ~ 20)
Sugar juice, is then the sodium periodate solution by volume 10 of 0.11g/mL by natural polysaccharide solution and concentration:1 is well mixed,
The pH to 3.0 of above-mentioned mixed liquor is adjusted with the sulfuric acid solution of 1mol/L afterwards, then by mixed liquor under 40 DEG C of dark condition
Stirring reaction 4h, finally using being dried to obtain hydroformylation polysaccharide at ethanol precipitation product, 37 DEG C；
(2) preparation of oxidation of polysaccharides：By hydroformylation polysaccharide and 30% hydrogen peroxide in mass ratio 1:(0.1 ~ 0.6) it is well mixed,
Then it is 1 to press copper sulphate with hydroformylation polysaccharide dry weight ratio:2000 ratio adds 1% copper sulfate solution, 70 after being well mixed
DEG C ~ 80 DEG C under the conditions of react 10 ~ 20min, the deionized water of 10 ~ 20 times of hydroformylation polysaccharide dry weight is subsequently adding, in 70 DEG C ~ 80 DEG C bars
Continue to react 30 ~ 50min under part, oxidation of polysaccharides is obtained after absolute ethyl alcohol precipitation, 37 DEG C of dryings；
(3) by oxidation of polysaccharides and sterilized water according to mass ratio (1 ~ 20):500 mixing, stir 0.5 ~ 2h, Ran Houleng at 50 DEG C
But it is made oxidation of polysaccharides solution；
(4) disodium hydrogen phosphate is added in oxidation of polysaccharides solution, regulation oxidation of polysaccharides pH value of solution scope between 5.4 ~ 7.8,
Then glycerine is added in oxidation of polysaccharides solution, obtains mixture；
(5) said mixture is stirred into 0.5 ~ 2h at 25 DEG C, obtains final product new medical antimicrobial spray.
The oxidation of polysaccharides mean molecule quantity be 30000 ~ 250000, in its molecular structure simultaneously containing aldehyde radical, carboxyl and
Three kinds of functional groups of hydroxyl, and in oxidation of polysaccharides molecule aldehyde radical mass fraction between 0.1 ~ 15%, carboxyl in oxidation of polysaccharides molecule
Mass fraction between 0.1 ~ 10%.
Wherein the mass ratio of oxidation of polysaccharides, disodium hydrogen phosphate, glycerine and sterilized water is (1 ~ 20): (10~20): 20:
500。
Advantages of the present invention can inductive generalization it is as follows：
(1) antimicrobial component that the present invention is used is oxidation of polysaccharides, and oxidation of polysaccharides can be by the oxidation system of natural polysaccharide
Standby, current mature preparation process can largely obtain cheap raw material, also, state modulator oxidation that can be by adjusting technique is more
The content of aldehyde radical and carboxyl in glycan molecule；
(2) water solubility of oxidation of polysaccharides is significantly larger than natural polysaccharide, hence in so that range of application of the oxidation of polysaccharides in water environment
Significantly larger than natural polysaccharide.In the preparation of spray, oxidation of polysaccharides solubility is high, adds the viscous of the mixed system after oxidation of polysaccharides
Degree is low, beneficial to the sprinkling of mixed liquor；
(3) oxidation of polysaccharides antimicrobial spray can form one layer of oxidation of polysaccharides protective layer in wound surface, can prevent the intrusion of bacterium.
Meanwhile, a certain proportion of aldehyde radical and carboxyl being contained in oxidation of polysaccharides, under the synergy of the two groups, can effectively kill
The bacterium of wound surface of going out.The cytotoxicity of oxidation of polysaccharides is extremely low, with preferable biocompatibility, is covered in wound surface not
Wound healing can be adversely affected；
(4) the obtained new medical antimicrobial spray of the present invention, preparation process is simple, raw material is inexpensively environmentally friendly, with excellent antibacterial
Performance, and wound is had no toxic side effect, possess very big application prospect in pharmaceuticals industry.
Specific embodiment
Four embodiments of the invention are given below, the present invention is specifically described by embodiment.It is necessary herein
, it is noted that embodiment is served only for being further detailed the present invention, it is impossible to be interpreted as the limit to the scope of the present invention
System, the person skilled in the art in the field can make some nonessential modifications and adaptations according to the content of the invention described above.
Embodiment one
Dry amylose 10g is weighed, dissolving in 200mL deionized waters is added to, is subsequently adding the 0.11g/mL's of 20mL
Sodium periodate solution, the pH to 3.0 of mixed liquor is adjusted with the sulfuric acid of 1mol/L, and mixed reactant is 40 oStirred under the dark condition of C
4h is mixed, is precipitated with absolute ethyl alcohol, once oxidation amylose is dried to obtain at 37 DEG C.5g once oxidation amyloses are taken, plus
Enter the hydrogen peroxide of 2mL 30%, the copper sulfate solution of 0.25mL 1% reacts 10min after being well mixed under the conditions of 70 DEG C,
The deionized water of 100mL is subsequently adding, 30min is reacted under the conditions of 70 DEG C, precipitated through absolute ethyl alcohol, be dried to obtain oxidation straight chain
Starch.Weigh 1g oxidation amyloses to be added in 50mL sterilized waters, 0.5h is stirred at 50 DEG C, then cool down, by phosphoric acid hydrogen
Disodium is added in oxidation of polysaccharides solution, adjusts pH value of solution, makes its pH scope between 5.4 ~ 7.8, is weighed 2g glycerine and is added to
In mixture, 0.5h is stirred at 25 DEG C, obtain new medical antimicrobial spray.
Embodiment two
Dry shitosan 20g is weighed, dissolving in 300mL deionized waters is added to, the height of the 0.11g/mL of 30mL is subsequently adding
Sodium iodide solution, the pH to 3.0 of mixed liquor is adjusted with the sulfuric acid of 1mol/L, and mixed reactant is 40 oStirred under the dark condition of C
4h, is precipitated with absolute ethyl alcohol, and once oxidation shitosan is dried to obtain at 37 DEG C.5g once oxidation shitosans are taken, 2mL is added
30% hydrogen peroxide, the copper sulfate solution of 0.25mL 1% reacts 10min, Ran Houjia after being well mixed under the conditions of 70 DEG C
Enter the deionized water of 200mL, 30min is reacted under the conditions of 70 DEG C, precipitated through absolute ethyl alcohol, be dried to obtain oxidation shitosan.Claim
Take 5g oxidation shitosans to be added in 150mL sterilized waters, 2h is stirred at 50 DEG C, then cool down, disodium hydrogen phosphate is added to
In oxidation chitosan solution, pH value of solution is adjusted, makes its pH scope between 5.4 ~ 7.8, weighed 6g glycerine and be added in mixture,
1h is stirred at 25 DEG C, new medical antimicrobial spray is obtained.
Embodiment three
Dry sodium alginate 10g is weighed, dissolving in 150mL deionized waters is added to, is subsequently adding the 0.11g/mL's of 15mL
Sodium periodate solution, the pH to 3.0 of mixed liquor is adjusted with the sulfuric acid of 1mol/L, and mixed reactant is 40 oStirred under the dark condition of C
4h is mixed, is precipitated with absolute ethyl alcohol, once oxidation sodium alginate is dried to obtain at 37 DEG C.5g once oxidation sodium alginates are taken, plus
Enter the hydrogen peroxide of 2mL 30%, the copper sulfate solution of 0.25mL 1% reacts 10min after being well mixed under the conditions of 70 DEG C,
The deionized water of 100mL is subsequently adding, 30min is reacted under the conditions of 70 DEG C, precipitated through absolute ethyl alcohol, be dried to obtain oxidation marine alga
Sour sodium.Weigh 1g oxidized sodium alginates to be added in 50mL sterilized waters, 1h is stirred at 50 DEG C, then cool down, by phosphoric acid hydrogen two
Sodium is added in oxidized sodium alginate solution, adjusts pH value of solution, makes its pH scope between 5.4 ~ 7.8, weighs the addition of 2g glycerine
To in mixture, 2h is stirred at 25 DEG C, obtain new medical antimicrobial spray.
Example IV
Dry guar gum 10g is weighed, dissolving in 200mL deionized waters is added to, the height of the 0.11g/mL of 20mL is subsequently adding
Sodium iodide solution, the pH to 3.0 of mixed liquor is adjusted with the sulfuric acid of 1mol/L, and mixed reactant is 40 oStirred under the dark condition of C
4h, is precipitated with absolute ethyl alcohol, and once oxidation guar gum is dried to obtain at 37 DEG C.5g once oxidation guar gums are taken, 2mL is added
30% hydrogen peroxide, the copper sulfate solution of 0.25mL 1% reacts 20min, Ran Houjia after being well mixed under the conditions of 80 DEG C
Enter the deionized water of 100mL, 50min is reacted under the conditions of 80 DEG C, precipitated through absolute ethyl alcohol, be dried to obtain oxidized guar.Claim
Take 2g oxidized guars to be added in 100mL sterilized waters, 1h is stirred at 50 DEG C, then cool down, disodium hydrogen phosphate is added to
In oxidized guar solution, pH value of solution is adjusted, makes its pH scope between 5.4 ~ 7.8, weighed 4g glycerine and be added in mixture,
1h is stirred at 25 DEG C, new medical antimicrobial spray is obtained.
Claims (3)
1. a kind of new medical antimicrobial spray, it is characterised in that be made up of oxidation of polysaccharides, disodium hydrogen phosphate, glycerine and sterilized water,
Its pH scope is between 5.4 ~ 7.8, and its specific preparation method is as follows：
(1) preparation of hydroformylation polysaccharide：In mass ratio 1:Be dissolved in natural polysaccharide in deionized water and obtain natural many by (10 ~ 20)
Sugar juice, is then the sodium periodate solution by volume 10 of 0.11g/mL by natural polysaccharide solution and concentration:1 is well mixed,
The pH to 3.0 of above-mentioned mixed liquor is adjusted with the sulfuric acid solution of 1mol/L afterwards, then by mixed liquor under 40 DEG C of dark condition
Stirring reaction 4h, finally using being dried to obtain hydroformylation polysaccharide at ethanol precipitation product, 37 DEG C；
(2) preparation of oxidation of polysaccharides：By hydroformylation polysaccharide and 30% hydrogen peroxide in mass ratio 1:(0.1 ~ 0.6) it is well mixed,
Then it is 1 to press copper sulphate with hydroformylation polysaccharide dry weight ratio:2000 ratio adds 1% copper sulfate solution, 70 after being well mixed
DEG C ~ 80 DEG C under the conditions of react 10 ~ 20min, the deionized water of 10 ~ 20 times of hydroformylation polysaccharide dry weight is subsequently adding, in 70 DEG C ~ 80 DEG C bars
Continue to react 30 ~ 50min under part, oxidation of polysaccharides is obtained after absolute ethyl alcohol precipitation, 37 DEG C of dryings；
(3) by oxidation of polysaccharides and sterilized water according to mass ratio (1 ~ 20):500 mixing, stir 0.5 ~ 2h, Ran Houleng at 50 DEG C
But it is made oxidation of polysaccharides solution；
(4) disodium hydrogen phosphate is added in oxidation of polysaccharides solution, regulation oxidation of polysaccharides pH value of solution scope between 5.4 ~ 7.8,
Then glycerine is added in oxidation of polysaccharides solution, obtains mixture；
(5) said mixture is stirred into 0.5 ~ 2h at 25 DEG C, obtains final product new medical antimicrobial spray.
2. new medical antimicrobial spray according to claim 1, it is characterised in that the oxidation of polysaccharides mean molecule quantity is
30000 ~ 250000, contain aldehyde radical, three kinds of functional groups of carboxyl and hydroxyl, and aldehyde in oxidation of polysaccharides molecule in its molecular structure simultaneously
Between 0.1 ~ 15%, the mass fraction of carboxyl is between 0.1 ~ 10% in oxidation of polysaccharides molecule for the mass fraction of base.
3. new medical antimicrobial spray according to claim 1, it is characterised in that oxidation of polysaccharides, disodium hydrogen phosphate, glycerine
It is (1 ~ 20) with the mass ratio of sterilized water: (10~20): 20: 500.
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| CN201710027863.1A CN106821979A (en) | 2017-01-16 | 2017-01-16 | A kind of new medical antimicrobial spray |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101035572A (en) * | 2004-10-07 | 2007-09-12 | 纳幕尔杜邦公司 | Polysaccharide-based polymer tissue adhesive for medical use |
| CN105457107A (en) * | 2016-01-12 | 2016-04-06 | 四川大学 | Double-functional-layer oral restoration membrane and preparation method thereof |
| CN105831550A (en) * | 2016-04-12 | 2016-08-10 | 四川大学 | A novel food bacteriostatic agent and a preparing method thereof |
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- 2017-01-16 CN CN201710027863.1A patent/CN106821979A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101035572A (en) * | 2004-10-07 | 2007-09-12 | 纳幕尔杜邦公司 | Polysaccharide-based polymer tissue adhesive for medical use |
| CN105457107A (en) * | 2016-01-12 | 2016-04-06 | 四川大学 | Double-functional-layer oral restoration membrane and preparation method thereof |
| CN105831550A (en) * | 2016-04-12 | 2016-08-10 | 四川大学 | A novel food bacteriostatic agent and a preparing method thereof |
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Application publication date: 20170613 |