CN106818799A - A kind of preparation method of the bovine serum albumin(BSA) fenoxycarb Nano medication for preventing and treating ditch socket of the eye elephant - Google Patents
A kind of preparation method of the bovine serum albumin(BSA) fenoxycarb Nano medication for preventing and treating ditch socket of the eye elephant Download PDFInfo
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- CN106818799A CN106818799A CN201710025122.XA CN201710025122A CN106818799A CN 106818799 A CN106818799 A CN 106818799A CN 201710025122 A CN201710025122 A CN 201710025122A CN 106818799 A CN106818799 A CN 106818799A
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- fenoxycarb
- bsa
- bovine serum
- serum albumin
- albumin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
Abstract
The invention discloses the preparation method that a kind of bovine serum albumin(BSA) for preventing and treating ditch socket of the eye elephant wraps up fenoxycarb Nano medication.The process of the bovine serum albumin(BSA) parcel fenoxycarb of described preventing and treating ditch socket of the eye elephant employs physically trapping method.First, a certain amount of bovine serum albumin(BSA) is taken（BSA）It is dissolved in distilled water and is configured to water phase, takes a certain amount of fenoxycarb and be dissolved in acetonitrile and be configured to oil phase.Then, under the conditions of high-speed stirred, oil phase is added dropwise in water phase, after emulsification a period of time, rapid cooling, Ultrasonic Pulverization obtains microemulsion, cools down.Finally, it is cold dry to obtain fenoxycarb albumin nano particle.The Novel albumin nano-particle technology of preparing that the method is used is on the premise of albumin structure is not changed, medicine to be wrapped in albumin, increased drugloading rate, while also having the effect of sustained release.Consumption, residual quantity, cost and the environmental contamination of chemical pesticide can be reduced using nanometer technology, while drug effect can also be improved, the contact area of medicament and target pest is effectively increased, the efficient of chemical pesticide, low toxicity, safety is really realized.
Description
Technical field
The invention discloses a kind of preparation method of the bovine serum albumin(BSA)-fenoxycarb Nano medication for preventing and treating ditch socket of the eye elephant, benzene
The preparation process of oxygen prestige nanoparticle, belongs to life science, chemistry, nano material and novel agrochemical technical field.
Background technology
Fenoxycarb, English nameEthyl 2-(4-phenoxyphenoxy)ethylcarbamate, molecular formula is
C17H19NO4, fenoxycarb, ABG-6215 or Fenoxycarb 25WG, benzene are the non-terpenes insect growth regulator, IGR classes with juvenile hormone activity
Insecticide, is put on market by Syngenta Co., Ltd of Switzerland in eighties of last century the nineties earliest, and is classified as two Pius XIs by first just reaching
One of more than ten retaining variety recorded.Fenoxycarb sterling is colourless crystallization body, 53-54 DEG C of fusing point, dissolubility (at 25 DEG C)：Water
It is more than 250g/Kg in 5.7mg/Kg, acetone, chloroform, ether ethyl acetate, methyl alcohol, isopropanol, toluene etc..In pH3,7,9,50
Hydrolysis-stable at DEG C, to light stabilization, low toxicity, efficiently, it is environmentally safe.It is wide with insecticidal spectrum, it is dynamic to lactation to pest efficient
Thing low toxicity, harmless to natural enemy, the lasting period is long, the advantages of environmentally safe.Because fenoxycarb has diphenyl ether and carbamic acid concurrently
Ester molecular structure, makes it have various insecticidal functions, tool stomach toxicity and action of contace poison and have various regulation work(to insect endocrine hormone
Energy.Mechanism of action is：Make insect cannot molt metamorphosis and it is gradually dead；Suppress adult stage metamorphosis, so as to cause later stage or pupa time
It is dead；There is stronger ovicidal action, insect population number can be reduced；Embryonic development, breeding and the extra-sexual hormone of insect can be changed.Extensively should
For grain storage pest（Rice weevil, sitophilus zea-mais, grain weevil, red flour beetle, Indian meal moth and gelechiid etc.）, injurious forest-insect（Pine moth, ditch socket of the eye
As with willow multiple leafeating insects）, fruit tree pest insect（Base of a fruit moth, lychee stink-bug, pear sucker and apple yellow aphid etc.）, fruits and vegetables insect
（Cabbage caterpillar, vegetable aphid and Liriomyza etc.）And sanitary insect pest（Termite, cockroach, flea and ant etc.）Deng the insect of multiple types.In vain
Albumen has the advantages that safety non-toxic, non-immunogenicity, biodegradable and good biocompatibility, is a kind of preferably administration system
System carrier material.The features such as biodegradable, nontoxic, no antigen and bioavilability high for having, albumin has been received
The attention of biomedicine field research worker, is widely used as diagnostic reagent and pharmaceutical carrier etc..Novel albumin nanometer system
Standby technology is on the premise of albumin structure is not changed, medicine to be loaded into wherein, and medicine is with the " pure of Nano grade in supensoid agent
Medicine " particulate forms are present, for slightly solubility, heavy dose of drug injection administration provide a kind of new approach.Using embedding techniques
Pure medicament nano particle is made nano biological agricultural chemicals microballoon, bulk effect, skin effect, amount specific to nano material is played
Sub- dimensional effect and macro quanta tunnel effect, significantly increase drug effect, traditional insecticide-applying way of agricultural chemicals be by spray pattern,
To be lost in soil and air by the effective ingredient of this process 80-90%, directly result in the formulation rate of agricultural chemicals
Increasing, spraying times increase etc..The pesticide granules or liquid of submicron order are wrapped in by an inertia by using nanometer technology
In shell structure or it is mounted on a kind of macromolecular substances, forms a kind of new nano pesticide.Being truly realized chemical pesticide makes
It is low concentration, low dose, low cost, hypotoxicity, low resistance, low-residual, broad spectrum disinfestation, efficient and environmentally safe.
The content of the invention
It is an object of the invention to provide a kind of novel agrochemical bovine serum albumin(BSA)-fenoxycarb nano-particle for preventing and treating ditch socket of the eye elephant
Preparation method, the method use Novel albumin nano fabrication technique be on the premise of albumin structure is not changed, will
Medicine is loaded into wherein, is more easy to be eaten and absorbed, and preferably plays and tags and stomach poison function, improves drug effect, reduces consumption, carries medicine
Amount is big, with controlled release ability, realizes the efficient of biological pesticide, low toxicity, safety.At present, not yet about nanoscale fenoxycarb medicine
Research report.The method has can be miniaturized Pesticidal products, reduces cost, and can reach " low consumption, high benefit " can
Sustainable development purpose, realizes the ensured sustained development of the utilization of resources.
Above-mentioned purpose of the invention is to be achieved through the following technical solutions：Bovine serum albumin(BSA) wraps up the process of fenoxycarb
Emulsification solidification method is employed, albumin nano granular is used as a kind of pharmaceutical carrier, non-immunogenicity, good biocompatibility.
The process of described bovine serum albumin(BSA) parcel fenoxycarb employs emulsification solidification method.First, a certain amount of ox blood is taken
Pure albumen（BSA）It is dissolved in distilled water and is configured to water phase, takes a certain amount of fenoxycarb and be dissolved in acetonitrile and be configured to oil phase.Then, exist
Under the conditions of high-speed stirred, oil phase is added dropwise in water phase, after emulsification a period of time, rapid cooling, Ultrasonic Pulverization obtains ultra micro
Emulsion, cooling.Finally, it is cold dry to obtain fenoxycarb albumin nano particle.The Novel albumin nano-particle system that the method is used
Standby technology is on the premise of albumin structure is not changed, medicine to be wrapped in albumin, drugloading rate is increased, while also having
There is sustained release.Comprise the following steps that：
（1）Take a certain amount of bovine serum albumin(BSA) to be dissolved in distilled water, be configured to water phase, 50 DEG C of water-bath 30min obtain clarification saturating
Bright Bovine Serum Albumin in Aqueous Solution；
（2）A certain amount of fenoxycarb is taken, acetonitrile ultrasonic dissolution 5-20min is added, the fenoxycarb-acetonitrile of 2-15mg/mL is configured to
Oil phase；
（3）Water is mutually adjusted rotating speed 1000-5000 rpm, fenoxycarb-acetonitrile oil phase is added dropwise under high-speed stirred, emulsify 30-
60min, it is rapid in 5-10min is cooled down at 0-4 DEG C to take out emulsion, then takes out ultrasonic 5-20min, obtain bovine serum albumin(BSA)-
Fenoxycarb microemulsion；
（4）By bovine serum albumin(BSA)-fenoxycarb microemulsion through dialysis membrane（MWCO=5000）Dialysed overnight 12h；
（5）Dialyzate is taken out and freezed, it is cold it is dry obtain white powder, i.e. bovine serum albumin(BSA)-fenoxycarb nanoparticle.
Described bovine serum albumin(BSA) parcel object fenoxycarb, is hydrophobic drug, is applied equally to other hydrophobicitys
Medicine such as thiacloprid, AVM etc..
Described emulsification solidification method refers to that oil phase medicine is added in water phase albumin, under high-speed stirred, forms water bag
Oily nanoparticle, is ultrasonically formed stabilization, uniform ultra micro breast after ice bath, dialysis, freezing is cold to do to obtain bovine serum albumin(BSA)-benzene
Oxygen prestige nanoparticle.
Step（2）In fenoxycarb it is also interchangeable do other drugs replacement, its packaging method is also applicable.
Step（2）In acetonitrile also can be with ether, dichloromethane, chloroform, dimethyl sulfoxide (DMSO), petroleum ether, ethanol, methyl alcohol etc.
Various organic solvents are substituted.
Step（2）In ultrasonic time 5-20min, resulting nanoparticle uniform particle sizes, good dispersion.
Step（3）In emulsion process time 30-60min, the time, too short emulsification was insufficient, it is oversize can cause it is more poly-
Collection.
The present invention has advantages below：（1）Reaction mechanism of the invention is that water dropwise adds in the state of high speed rotates
Enter oil phase, produce oil-in-water emulsion droplet.
（2）The features such as albumin has biodegradable, nontoxic, no antigen and bioavilability high, medicine in itself is carried
Enter wherein, be more easy to be eaten and absorbed, preferably play and tag and stomach poison function, improve drug effect.
（3）The drugloading rate of albumin nano granular is big, with controlled release ability, realizes the efficient of biological pesticide, low toxicity, safety.
（4）The present invention does not need special chemical reagent, and process is simple, equipment requirement is low, and environmental protection is low to be manufactured into
This.
Brief description of the drawings
Fig. 1 schemes for the SEM of bovine serum albumin(BSA)-fenoxycarb nano-particle.
Fig. 2 is the effect that bovine serum albumin(BSA)-fenoxycarb nano pesticide prevents and treats ditch socket of the eye elephant.
Specific embodiment
（1）Take a certain amount of bovine serum albumin(BSA) to be dissolved in distilled water, be configured to water phase, 50 DEG C of water-bath 30min obtain clear
Clear bright Bovine Serum Albumin in Aqueous Solution；
（2）A certain amount of fenoxycarb is taken, acetonitrile ultrasonic dissolution 5-20min is added, the fenoxycarb-acetonitrile of 2-15mg/mL is configured to
Oil phase；
（3）Water is mutually adjusted rotating speed 1000-5000 rpm, fenoxycarb-acetonitrile oil phase is added dropwise under high-speed stirred, emulsify 30-
60min, it is rapid in 5-10min is cooled down at 0-4 DEG C to take out emulsion, then takes out ultrasonic 5-20min, obtain bovine serum albumin(BSA)-
Fenoxycarb microemulsion；
（4）By bovine serum albumin(BSA)-fenoxycarb microemulsion through dialysis membrane（MWCO=5000）Dialysed overnight 12h；
（5）Dialyzate is taken out and freezed, it is cold it is dry obtain white powder, i.e. bovine serum albumin(BSA)-fenoxycarb nanoparticle；
（6）The bovine serum albumin(BSA) that will be obtained-fenoxycarb nanoparticle is dissolved in pure water, is loaded into miniaturised nebuliser, and spray
Onto the food of ditch socket of the eye elephant, the death condition of ditch socket of the eye elephant is observed.The death condition of ditch socket of the eye elephant is shown in Fig. 2.
Embodiment 1
（1）0.08g bovine serum albumin(BSA)s are taken in 4ml distilled waters, water phase is configured to, 50 DEG C of water-bath 30min obtain clear
Bovine Serum Albumin in Aqueous Solution；
（2）It is another to take 1mg fenoxycarbs, 0.167ml acetonitrile ultrasonic dissolution 5min are added, it is configured to the fenoxycarb-acetonitrile oil of 6mg/mL
Phase；
（3）Water is mutually adjusted and be added dropwise under rotating speed 1000rpm, high-speed stirred fenoxycarb-acetonitrile oil phase, emulsify 30min, taken out
Emulsion is rapid to then take out ultrasonic 10min in 10min is cooled down at 0-4 DEG C, obtains bovine serum albumin(BSA)-fenoxycarb microemulsion；
（4）By microemulsion dialysis membrane（MWCO=5000）Dialysed overnight, take out dialyzate freezing, it is cold it is dry obtain white powder, i.e.,
Fenoxycarb albumin nano granular；
（5）It is cold dry to obtain fenoxycarb albumin nano granular.
Embodiment 2
（1）0.08g bovine serum albumin(BSA)s are taken in 4ml distilled waters, water phase is configured to, 50 DEG C of water-bath 30min obtain clear
Bovine Serum Albumin in Aqueous Solution；
（2）It is another to take 2mg fenoxycarbs, 0.334ml acetonitrile ultrasonic dissolution 5min are added, it is configured to the fenoxycarb-acetonitrile oil of 6mg/mL
Phase；
（3）Water is mutually adjusted and be added dropwise under rotating speed 1000rpm, high-speed stirred fenoxycarb-acetonitrile oil phase, emulsify 40min, taken out
Emulsion is rapid to then take out ultrasonic 10min in 10min is cooled down at 0-4 DEG C, obtains bovine serum albumin(BSA)-fenoxycarb microemulsion；
（4）By microemulsion dialysis membrane（MWCO=5000）Dialysed overnight, take out dialyzate freezing, it is cold it is dry obtain white powder, i.e.,
Fenoxycarb albumin nano granular.
Embodiment 3
（1）0.08g bovine serum albumin(BSA)s are taken in 4ml distilled waters, water phase is configured to, 50 DEG C of water-bath 30min obtain clear
Bovine Serum Albumin in Aqueous Solution；
（2）It is another to take 3mg fenoxycarbs, 0.501ml acetonitrile ultrasonic dissolution 10min are added, it is configured to the fenoxycarb-acetonitrile oil of 6mg/mL
Phase；
（3）Water is mutually adjusted and be added dropwise under rotating speed 1200rpm, high-speed stirred fenoxycarb-acetonitrile oil phase, emulsify 40min, taken out
Emulsion is rapid to then take out ultrasonic 10min in 5min is cooled down at 0-4 DEG C, obtains bovine serum albumin(BSA)-fenoxycarb microemulsion；
（4）By microemulsion dialysis membrane（MWCO=5000）Dialysed overnight, take out dialyzate freezing, it is cold it is dry obtain white powder, i.e.,
Fenoxycarb albumin nano granular.
Embodiment 4
（1）0.08g bovine serum albumin(BSA)s are taken in 4ml distilled waters, water phase is configured to, 50 DEG C of water-bath 30min obtain clear
Bovine Serum Albumin in Aqueous Solution；
（2）It is another to take 4mg fenoxycarbs, 0.668ml acetonitrile ultrasonic dissolution 10min are added, it is configured to the fenoxycarb-acetonitrile oil of 6mg/mL
Phase；
（3）Water is mutually adjusted and be added dropwise under rotating speed 1200rpm, high-speed stirred fenoxycarb-acetonitrile oil phase, emulsify 50min, taken out
Emulsion is rapid to then take out ultrasonic 15min in 10min is cooled down at 0-4 DEG C, obtains bovine serum albumin(BSA)-fenoxycarb microemulsion；
（4）By microemulsion dialysis membrane（MWCO=5000）Dialysed overnight, take out dialyzate freezing, it is cold it is dry obtain white powder, i.e.,
Fenoxycarb albumin nano granular.
Embodiment 5
（1）0.08g bovine serum albumin(BSA)s are taken in 4ml distilled waters, water phase is configured to, 50 DEG C of water-bath 30min obtain clear
Bovine Serum Albumin in Aqueous Solution；
（2）It is another to take 5mg fenoxycarbs, 0.835ml acetonitrile ultrasonic dissolution 15min are added, it is configured to the fenoxycarb-acetonitrile oil of 6mg/mL
Phase；
（3）Water is mutually adjusted and be added dropwise under rotating speed 1500rpm, high-speed stirred fenoxycarb-acetonitrile oil phase, emulsify 50min, taken out
Emulsion is rapid to then take out ultrasonic 15min in 10min is cooled down at 0-4 DEG C, obtains bovine serum albumin(BSA)-fenoxycarb microemulsion；
（4）By microemulsion dialysis membrane（MWCO=5000）Dialysed overnight, take out dialyzate freezing, it is cold it is dry obtain white powder, i.e.,
Fenoxycarb albumin nano granular.
Claims (5)
1. it is a kind of prevent and treat ditch socket of the eye elephant bovine serum albumin(BSA)-fenoxycarb Nano medication preparation method, it is characterised in that：
The process of described bovine serum albumin(BSA) parcel fenoxycarb employs emulsification solidification method, first, takes a certain amount of ox blood pure
Albumen（BSA）It is dissolved in distilled water and is configured to water phase, take a certain amount of fenoxycarb and be dissolved in acetonitrile and be configured to oil phase, then, at a high speed
Under stirring condition, oil phase is added dropwise in water phase, after emulsification a period of time, rapid cooling, Ultrasonic Pulverization obtains microemulsion,
Cooling, finally, it is cold it is dry obtain fenoxycarb albumin nano particle, the Novel albumin nano-particle technology of preparing that the method is used
It is on the premise of albumin structure is not changed, medicine to be wrapped in albumin, increased drugloading rate, while also has sustained release
Effect, comprise the following steps that：
（1）Take a certain amount of bovine serum albumin(BSA) to be dissolved in distilled water, be configured to water phase, 50 DEG C of water-bath 30min obtain clarification saturating
Bright Bovine Serum Albumin in Aqueous Solution；
（2）A certain amount of fenoxycarb is taken, acetonitrile ultrasonic dissolution 5-20min is added, the fenoxycarb-acetonitrile of 2-15mg/mL is configured to
Oil phase；
（3）Water is mutually adjusted rotating speed 1000-5000 rpm, fenoxycarb-acetonitrile oil phase is added dropwise under high-speed stirred, emulsify 30-
60min, it is rapid in 5-10min is cooled down at 0-4 DEG C to take out emulsion, then takes out ultrasonic 5-20min, obtain bovine serum albumin(BSA)-
Fenoxycarb microemulsion；
（4）By bovine serum albumin(BSA)-fenoxycarb microemulsion through dialysis membrane（MWCO=5000）Dialysed overnight 12h；
（5）Dialyzate is taken out and freezed, it is cold it is dry obtain white powder, i.e. bovine serum albumin(BSA)-fenoxycarb nanoparticle.
2. it is according to claim 1 it is a kind of prevent and treat ditch socket of the eye elephant bovine serum albumin(BSA)-fenoxycarb Nano medication preparation side
Method, it is characterised in that：The oil phase is added dropwise over the process rotating speed 1000-5000 rpm of water phase.
3. it is according to claim 1 it is a kind of prevent and treat ditch socket of the eye elephant bovine serum albumin(BSA)-fenoxycarb Nano medication preparation side
Method, it is characterised in that：
The acetonitrile also can be various organic molten with ether, dichloromethane, chloroform, dimethyl sulfoxide (DMSO), petroleum ether, ethanol, methyl alcohol etc.
Agent is substituted.
4. it is according to claim 1 it is a kind of prevent and treat ditch socket of the eye elephant bovine serum albumin(BSA)-fenoxycarb Nano medication preparation side
Method, it is characterised in that：
The emulsion process time 30-60min, the time, too short emulsification was insufficient, oversize to cause more aggregations.
5. it is according to claim 1 it is a kind of prevent and treat ditch socket of the eye elephant bovine serum albumin(BSA)-fenoxycarb Nano medication preparation side
Method, it is characterised in that：The ultrasonic time 5-20min, resulting nanoparticle uniform particle sizes, good dispersion.
Priority Applications (1)
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| CN201710025122.XA CN106818799B (en) | 2017-01-13 | 2017-01-13 | Preparation method of bovine serum albumin-fenoxycarb nano-drug for preventing and treating orbital elephant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710025122.XA CN106818799B (en) | 2017-01-13 | 2017-01-13 | Preparation method of bovine serum albumin-fenoxycarb nano-drug for preventing and treating orbital elephant |
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| CN106818799B CN106818799B (en) | 2022-03-15 |
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