CN106771267A - Quick blood type design detects card - Google Patents

Quick blood type design detects card Download PDF

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Publication number
CN106771267A
CN106771267A CN201611243101.7A CN201611243101A CN106771267A CN 106771267 A CN106771267 A CN 106771267A CN 201611243101 A CN201611243101 A CN 201611243101A CN 106771267 A CN106771267 A CN 106771267A
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China
Prior art keywords
blood
blocks
blood typing
detection
adsorptive pads
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Pending
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CN201611243101.7A
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Chinese (zh)
Inventor
冯晓均
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Hubei Ganuo Beauty Biotechnology Co Ltd
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Wuhan Jianuomei Technology Co ltd
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Priority to CN201611243101.7A priority Critical patent/CN106771267A/en
Publication of CN106771267A publication Critical patent/CN106771267A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention belongs to the field of blood type typing detection, and particularly relates to a quick blood type typing detection card. The detection reagent strip consists of a sample pad, an upper water absorption pad and a lower water absorption pad, wherein the sample pad is arranged between the upper water absorption pad and the lower water absorption pad, the sample pad is easily absorbed into a buffer unit through the chromatography effect of the lower water absorption pad, the upper water absorption pad also has the chromatography effect on the sample pad, and thus, the blood type is determined through the color change caused by the blood type setting reaction of blood in the sample pad. The invention has the characteristics of portability, integration, cleanness, sanitation, quick shaping and easy operation.

Description

A kind of quick blood typing detection card
Technical field
The invention belongs to blood typing detection field, specifically a kind of portable, integrated quick blood typing Detection card.
Background technology
Blood group typically refers to the type of specific antigen on erythrocyte membrane, and the foundation of its classification is whether erythrocyte surface is deposited In some heritable antigenic substances.If the droplet of blood of two people of blood group incompatibility being added on slide and being allowed to mix, Red blood cell can aggegation cluster, this phenomenon is referred to as RCA.The mechanism for causing aggegation is antigen-antibody reaction.Red blood cell Surface also has specificity glycoprotein antigenic substance to be referred to as agglutinogen(Agglutinogen).Then containing corresponding in blood plasma Specific antibody, referred to as agglutinin(Agglutinin).Wherein commonly using blood group system has ABO systems and Rh systems.
Blood group is that human blood take blood group antigens as the inhereditary feature for showing shape, and most and common blood group systems is ABO Blood group(According to whetheing there is Staphylococal Protein A/and B antigens on red blood cell, blood group is divided into A types, Type B, AB types and O-shaped four kinds);Blood group is being faced Bed blood transfusion and blood genetic research are upper significant.When corresponding antigen meets with antibody in abo blood group, red blood cell is just Agglutinating reaction can occur, erythrocyte hemolysis, threat to life are ultimately resulted in.Therefore, Blood grouping is to ensure safe transfusion, avoid phase The important prerequisite condition that related disorders occur.
At present, existing some blood typing methods.Such as slide method, it is simple to operate, it is suitable to a large amount of sample inspections, but reaction Time is long, and those who are investigated's such as serum antibody titer is low, then be difficult to cause RCA;Test tube method:Can be with by means of centrifugal force Contact red blood cell tight, promote agglutination, but instrument needs special messenger to operate.Therefore, current existing blood typing method It is difficult to meet the demand of each field particularly clinical field instantly.
The content of the invention
In view of the shortcomings of the prior art, the invention provides a kind of quick blood typing detection card.Detection examination of the invention Agent bar is made up of sample pad, upper adsorptive pads and lower adsorptive pads, and sample pad is arranged between adsorptive pads and lower adsorptive pads, under By the easy suction sample pad in buffer cell, upper adsorptive pads are same to there is chromatography to make sample pad for the chromatography effect of adsorptive pads With so the color change for causing is reacted in the blood typing by blood in sample pad carries out blood group judgement.The present invention has Portable, integrated, clean hygiene, quick and easy-operating feature of shaping.
The technical scheme is that:A kind of quick blood typing detection card, including sizing detection unit and buffer cell, It is characterized in that:Described sizing detection unit is made up of upper shell, lower house and detection reagent bar, and upper shell is provided with sample-adding Hole, lower house is provided with locating slot, and locating slot is used to for detection reagent bar to be fixedly mounted on lower house, described detection reagent bar It is made up of sample pad, upper adsorptive pads and lower adsorptive pads, sample pad is arranged between adsorptive pads and lower adsorptive pads;Sample pad is just right Well, upper shell, the box body downside of lower house composition are provided with patchhole, and buffer cell is arranged in the hole, lower adsorptive pads Contacted with buffer cell.
According to quick blood typing detection card as described above, it is characterised in that:Described buffer cell by Buffer Pool with And its internal cushioning liquid composition, lower adsorptive pads and buffer solution contact are contained in, Buffer Pool can be inserted into upper shell, lower house Patchhole on the downside of the box body of composition.
According to quick blood typing detection card as described above, it is characterised in that:Coating in described sample pad is set There are anti-A, B or Rh antibody and preservative.
According to quick blood typing detection card as described above, it is characterised in that:Also include sampling unit;Described sampling Unit is made up of capillary and rubber suction nozzle, and capillary is arranged on the side of rubber suction nozzle.
According to quick blood typing detection card as described above, it is characterised in that:Described capillary by hydrophilic plastic or Person's glass material is constituted.
According to quick blood typing detection card as described above, it is characterised in that:Described capillary diameter of inner pipe is 1.35 ± 0.1mm, every 5 microlitres of scales.
According to quick blood typing detection card as described above, it is characterised in that:Described rubber suction nozzle uses rubber material Material composition, its diameter of inner pipe is identical with capillary diameter.
According to quick blood typing detection card as described above, it is characterised in that:Also include matching used with blood type card Detector;Described detector is made up of optically detecting unit, image-signal processor and electronics peripheral hardware, is read by optical detection Take blood typing detection card result.
Technique effect of the invention is embodied in:The present invention realizes the fast of blood group from user's angle by solid phase method Speed sizing test.It is bold by reagent and combinations of getting stuck, make whole detection integration, not only convenient use person's carrying, while whole Easy to operate, the clean hygiene of individual detection.Only need 5 microlitres of blood sample, naked eyes can at 10 seconds or so quick interpretation bleeding type, For emergency judges that blood group has saved the time.The detection card of blood sample can will be also added to be put into matched inspection simultaneously Surveying in device carries out interpretation, and prints and carry out staying shelves to preserve, it is convenient after it is for future reference.The present invention meets each field to blood well The demand of type fast shaping.
Brief description of the drawings
Fig. 1 is abo blood group of the present invention sizing card front view;
Fig. 2 is abo blood group of the present invention sizing card rearview;
Fig. 3 is ABO and RhD blood typings card front view of the present invention;
Fig. 4 is ABO and RhD blood typings card rearview of the present invention;
Fig. 5 is side structure schematic diagram of the present invention;
Fig. 6 is sampling unit schematic diagram;
Fig. 7 is and the matching used detector schematic diagram of blood type card;
Fig. 8 is abo blood group sizing card upper shell schematic diagram;
Fig. 9 is abo blood group sizing card lower house schematic diagram;
Figure 10 is ABO and RhD blood typing card upper shell schematic diagrames;
Figure 11 is ABO and RhD blood typing card lower house schematic diagrames;
Figure 12 is buffer cell schematic diagram;
Figure 13 is detection reagent bar schematic diagram.
Description of reference numerals:Upper shell 1, upper adsorptive pads 2, lower house 3, well 4, sample pad 5, lower adsorptive pads 6, buffering Pond 7, rubber suction nozzle 8, capillary 9, optically detecting unit 10.
Specific embodiment
The present invention is done below in conjunction with the accompanying drawings further is described in detail.
Quick blood typing detection card of the invention, employs the fast shaping that solid phase method realizes blood group, including sizing inspection Survey unit and buffer cell, while also include sampling unit and with the matching used detector of blood type card.As shown in fig. 6, this hair Bright sampling unit, is made up of capillary 9 and rubber suction nozzle 8, using capillarity, quantitative collection trace sample.In the present invention, Capillary 9 is preferably made up of hydrophilic plastic or glass material, and its diameter of inner pipe is 1.35 ± 0.1mm, an every 5 microlitres quarters Degree, because only needing 5 microlitres per hole sample size, can so reduce the collection capacity of sample, and rubber suction nozzle 8 uses elastomeric material Composition, its diameter of inner pipe is identical with capillary diameter, and capillary can so be intimately associated with rubber suction nozzle, makes blood sampling more Plus it is convenient.
As shown in Fig. 1 to Fig. 5, Fig. 8 to Figure 13, it is of the invention sizing detection unit by upper shell 1, with upper shell clamping Lower house 3 and detection reagent bar are constituted, and wherein lower house 3 is provided with locating slot, and locating slot is fixedly mounted on for detection reagent bar On lower house 3.And upper shell 1, lower house 3 are used for common carrying detection reagent bar, detection reagent bar is by sample pad 5, upper water suction Pad 2 and lower adsorptive pads 6 are constituted.Lower adsorptive pads 6 are acted on by chromatography, and by buffer solution water conservancy diversion to sample pad 5, upper adsorptive pads 2 are same Sample is acted on by chromatography, by unreacted blood sample in sample pad 5 and the cushioning liquid water conservancy diversion by coming on the water conservancy diversion of lower adsorptive pads 6 To in upper adsorptive pads 2.Coating in inventive samples pad 5 is provided with anti-A, B or Rh antibody and its preservative, for blood typing Reaction, sample pad 5 faces setting well 4 on upper shell 1, is easy to sample-adding.The box of upper shell of the present invention 1, the composition of lower house 3 Body downside is provided with patchhole, and Buffer Pool 7 can be inserted into the patchhole, and after insertion, Buffer Pool 7 is contacted with lower adsorptive pads 6.The present invention is slow Rush pond 7 and be contained in its internal cushioning liquid composition buffer cell.
If Fig. 7 is the matching used detector of blood type card, it is by optically detecting unit 10, image-signal processor and correlation Electronics peripheral hardware is constituted, and is read blood typing by optical detection and is detected card result, including on LCD screen, and print Come.
The course of work of the invention is:When there is corresponding reaction time of blood coagulation, blood sample cannot be rinsed dry by cushioning liquid Only, the aobvious red of sample pad 5;Generation reaction time of blood coagulation, sample pad 5 shows colourless.Buffer cell is by 7 groups of cushioning liquid and Buffer Pool Into as long as buffer concentration is of substantially equal with human body cell liquid, you can keep the concentration of intraor extracellular equal, also will not Cell is risen brokenly or shrinkage, lead to not observation.Cushioning liquid is used for the detection reacted flushing of card of shaping, and Buffer Pool 7 is used for Carry cushioning liquid;With the matching used detector of blood type card by optically detecting unit 10, image-signal processor and mutually powered-down Sub- peripheral hardware composition, reads blood typing and detects card result by optical detection, including on LCD screen, and print Come.The present invention using solid phase method not only detect quickly, reagent and sample consumption it is few, while integrated design is easy to carry, grasps Make convenient, clean hygiene.A large amount of blood source shaping mechanisms such as blood station, hospital are applicable not only to, while being also applied for family, individuality Deng.
Example 1:Abo blood group is detected.
Operating procedure:
1st, blood type test card is placed horizontally on dry workbench;
2nd, with 10 microlitres of sampling unit fetching blood sample(At second graduation mark), respectively to 5 microlitres of samples of addition in two wells 4 This;
3rd, gently pick up blood type test card, during Buffer Pool 7 is firmly pushed housing by hand slightly, and detection card is vertically placed on work On platform(Buffer Pool one end is downward), stand 10 seconds or so, can naked eyes interpretation sizing result, blood type card can be also put into supporting with it In the detector draw-in groove for using, interpretation sizing result.
As it will be easily appreciated by one skilled in the art that the foregoing is only presently preferred embodiments of the present invention, it is not used to The limitation present invention, all any modification, equivalent and improvement made within the spirit and principles in the present invention etc., all should include Within protection scope of the present invention.

Claims (8)

1. a kind of quick blood typing detection blocks, including sizing detection unit and buffer cell, it is characterised in that:Described sizing Detection unit is made up of upper shell, lower house and detection reagent bar, and upper shell is provided with well, and lower house is provided with locating slot, Locating slot is used to for detection reagent bar to be fixedly mounted on lower house, described detection reagent bar by sample pad, upper adsorptive pads and Lower adsorptive pads composition, sample pad is arranged between adsorptive pads and lower adsorptive pads;Sample pad faces well, upper shell, under The box body downside of housing composition is provided with patchhole, and buffer cell is arranged in the hole, and lower adsorptive pads are contacted with buffer cell.
2. quick blood typing detection according to claim 1 blocks, it is characterised in that:Described buffer cell is by Buffer Pool And its internal cushioning liquid composition, lower adsorptive pads and buffer solution contact are contained in, Buffer Pool can be inserted into upper shell, lower casing Patchhole on the downside of the box body of body composition.
3. quick blood typing detection according to claim 1 and 2 blocks, it is characterised in that:Bag in described sample pad It is equipped with anti-A, B or Rh antibody and preservative.
4. quick blood typing detection according to claim 1 and 2 blocks, it is characterised in that:Also include sampling unit;It is described Sampling unit be made up of capillary and rubber suction nozzle, capillary is arranged on the side of rubber suction nozzle.
5. quick blood typing detection according to claim 4 blocks, it is characterised in that:Described capillary is by hydrophilic plastic Or glass material composition.
6. quick blood typing detection according to claim 4 blocks, it is characterised in that:Described capillary diameter of inner pipe is 1.35 ± 0.1mm, every 5 microlitres of scales.
7. quick blood typing detection according to claim 4 blocks, it is characterised in that:Described rubber suction nozzle uses rubber Material is constituted, and its diameter of inner pipe is identical with capillary diameter.
8. quick blood typing detection according to claim 1 blocks, it is characterised in that:Also include being supported the use with blood type card Detector;Described detector is made up of optically detecting unit, image-signal processor and electronics peripheral hardware, by optical detection Read blood typing detection card result.
CN201611243101.7A 2016-12-29 2016-12-29 Quick blood type design detects card Pending CN106771267A (en)

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Application Number Priority Date Filing Date Title
CN201611243101.7A CN106771267A (en) 2016-12-29 2016-12-29 Quick blood type design detects card

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Application Number Priority Date Filing Date Title
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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2250342A (en) * 1990-11-27 1992-06-03 Pall Corp Blood typing
CN1894584A (en) * 2003-07-09 2007-01-10 麦迪奥诊断产品有限公司 Device and method for simultaneously carrying out blood group determination, serum cross-check and antibody detection test
WO2009149615A1 (en) * 2008-06-10 2009-12-17 英科新创(厦门)科技有限公司 A method of quickly determining human abo/rh/mn blood type and a test kit thereof
US20100323343A1 (en) * 2009-05-11 2010-12-23 Nexus Dx, Inc. Methods and compositions for analyte detection
CN103502819A (en) * 2011-04-21 2014-01-08 西门子公司 Blood typing devices and methods for testing blood type
CN205027765U (en) * 2015-09-23 2016-02-10 英科新创(厦门)科技有限公司 Positive and negative design comprehensive testing card of blood ABO and rhD
CN206369741U (en) * 2016-12-29 2017-08-01 武汉伽诺美科技有限公司 Quick blood type design detects card

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2250342A (en) * 1990-11-27 1992-06-03 Pall Corp Blood typing
CN1894584A (en) * 2003-07-09 2007-01-10 麦迪奥诊断产品有限公司 Device and method for simultaneously carrying out blood group determination, serum cross-check and antibody detection test
WO2009149615A1 (en) * 2008-06-10 2009-12-17 英科新创(厦门)科技有限公司 A method of quickly determining human abo/rh/mn blood type and a test kit thereof
US20100323343A1 (en) * 2009-05-11 2010-12-23 Nexus Dx, Inc. Methods and compositions for analyte detection
CN103502819A (en) * 2011-04-21 2014-01-08 西门子公司 Blood typing devices and methods for testing blood type
CN205027765U (en) * 2015-09-23 2016-02-10 英科新创(厦门)科技有限公司 Positive and negative design comprehensive testing card of blood ABO and rhD
CN206369741U (en) * 2016-12-29 2017-08-01 武汉伽诺美科技有限公司 Quick blood type design detects card

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Effective date of registration: 20171116

Address after: 436070 Hubei Ezhou city Gedian Development Zone Construction Avenue 216 East Lake high new wisdom city 24-2-202 room

Applicant after: Hubei Ganuo beauty Biotechnology Co. Ltd.

Address before: 430074 Wuhan hi-tech Avenue, Wuhan, Hubei Province, bio City Innovation Park, C6 Dong Bei Lou 402

Applicant before: Wuhan Ganuo beauty Technology Co. Ltd.

RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20170531