CN106771267A - Quick blood type design detects card - Google Patents
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- CN106771267A CN106771267A CN201611243101.7A CN201611243101A CN106771267A CN 106771267 A CN106771267 A CN 106771267A CN 201611243101 A CN201611243101 A CN 201611243101A CN 106771267 A CN106771267 A CN 106771267A
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- blood
- blocks
- blood typing
- detection
- adsorptive pads
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- 210000004369 blood Anatomy 0.000 title claims abstract description 47
- 239000008280 blood Substances 0.000 title claims abstract description 47
- 238000013461 design Methods 0.000 title description 2
- 238000001514 detection method Methods 0.000 claims abstract description 52
- 239000000872 buffer Substances 0.000 claims abstract description 25
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 14
- 238000009582 blood typing Methods 0.000 claims description 33
- 230000000274 adsorptive effect Effects 0.000 claims description 26
- 239000000203 mixture Substances 0.000 claims description 13
- 238000004513 sizing Methods 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 9
- 238000005070 sampling Methods 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 6
- 230000003287 optical effect Effects 0.000 claims description 4
- 230000002093 peripheral effect Effects 0.000 claims description 4
- 239000007853 buffer solution Substances 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 239000003755 preservative agent Substances 0.000 claims description 3
- 230000002335 preservative effect Effects 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 10
- 238000004587 chromatography analysis Methods 0.000 abstract description 6
- 238000007493 shaping process Methods 0.000 abstract description 6
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 230000010354 integration Effects 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract 6
- 210000004027 cell Anatomy 0.000 description 11
- 238000010586 diagram Methods 0.000 description 9
- 210000003743 erythrocyte Anatomy 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- 239000000427 antigen Substances 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000007689 inspection Methods 0.000 description 3
- 230000035484 reaction time Effects 0.000 description 3
- 238000010532 solid phase synthesis reaction Methods 0.000 description 3
- 101710186708 Agglutinin Proteins 0.000 description 2
- 101710146024 Horcolin Proteins 0.000 description 2
- 101710189395 Lectin Proteins 0.000 description 2
- 101710179758 Mannose-specific lectin Proteins 0.000 description 2
- 101710150763 Mannose-specific lectin 1 Proteins 0.000 description 2
- 101710150745 Mannose-specific lectin 2 Proteins 0.000 description 2
- 239000000910 agglutinin Substances 0.000 description 2
- 230000000890 antigenic effect Effects 0.000 description 2
- 230000023555 blood coagulation Effects 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 230000004523 agglutinating effect Effects 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 201000001383 blood group incompatibility Diseases 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000013536 elastomeric material Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003617 erythrocyte membrane Anatomy 0.000 description 1
- 210000000887 face Anatomy 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to the field of blood type typing detection, and particularly relates to a quick blood type typing detection card. The detection reagent strip consists of a sample pad, an upper water absorption pad and a lower water absorption pad, wherein the sample pad is arranged between the upper water absorption pad and the lower water absorption pad, the sample pad is easily absorbed into a buffer unit through the chromatography effect of the lower water absorption pad, the upper water absorption pad also has the chromatography effect on the sample pad, and thus, the blood type is determined through the color change caused by the blood type setting reaction of blood in the sample pad. The invention has the characteristics of portability, integration, cleanness, sanitation, quick shaping and easy operation.
Description
Technical field
The invention belongs to blood typing detection field, specifically a kind of portable, integrated quick blood typing
Detection card.
Background technology
Blood group typically refers to the type of specific antigen on erythrocyte membrane, and the foundation of its classification is whether erythrocyte surface is deposited
In some heritable antigenic substances.If the droplet of blood of two people of blood group incompatibility being added on slide and being allowed to mix,
Red blood cell can aggegation cluster, this phenomenon is referred to as RCA.The mechanism for causing aggegation is antigen-antibody reaction.Red blood cell
Surface also has specificity glycoprotein antigenic substance to be referred to as agglutinogen(Agglutinogen).Then containing corresponding in blood plasma
Specific antibody, referred to as agglutinin(Agglutinin).Wherein commonly using blood group system has ABO systems and Rh systems.
Blood group is that human blood take blood group antigens as the inhereditary feature for showing shape, and most and common blood group systems is ABO
Blood group(According to whetheing there is Staphylococal Protein A/and B antigens on red blood cell, blood group is divided into A types, Type B, AB types and O-shaped four kinds);Blood group is being faced
Bed blood transfusion and blood genetic research are upper significant.When corresponding antigen meets with antibody in abo blood group, red blood cell is just
Agglutinating reaction can occur, erythrocyte hemolysis, threat to life are ultimately resulted in.Therefore, Blood grouping is to ensure safe transfusion, avoid phase
The important prerequisite condition that related disorders occur.
At present, existing some blood typing methods.Such as slide method, it is simple to operate, it is suitable to a large amount of sample inspections, but reaction
Time is long, and those who are investigated's such as serum antibody titer is low, then be difficult to cause RCA;Test tube method:Can be with by means of centrifugal force
Contact red blood cell tight, promote agglutination, but instrument needs special messenger to operate.Therefore, current existing blood typing method
It is difficult to meet the demand of each field particularly clinical field instantly.
The content of the invention
In view of the shortcomings of the prior art, the invention provides a kind of quick blood typing detection card.Detection examination of the invention
Agent bar is made up of sample pad, upper adsorptive pads and lower adsorptive pads, and sample pad is arranged between adsorptive pads and lower adsorptive pads, under
By the easy suction sample pad in buffer cell, upper adsorptive pads are same to there is chromatography to make sample pad for the chromatography effect of adsorptive pads
With so the color change for causing is reacted in the blood typing by blood in sample pad carries out blood group judgement.The present invention has
Portable, integrated, clean hygiene, quick and easy-operating feature of shaping.
The technical scheme is that:A kind of quick blood typing detection card, including sizing detection unit and buffer cell,
It is characterized in that:Described sizing detection unit is made up of upper shell, lower house and detection reagent bar, and upper shell is provided with sample-adding
Hole, lower house is provided with locating slot, and locating slot is used to for detection reagent bar to be fixedly mounted on lower house, described detection reagent bar
It is made up of sample pad, upper adsorptive pads and lower adsorptive pads, sample pad is arranged between adsorptive pads and lower adsorptive pads;Sample pad is just right
Well, upper shell, the box body downside of lower house composition are provided with patchhole, and buffer cell is arranged in the hole, lower adsorptive pads
Contacted with buffer cell.
According to quick blood typing detection card as described above, it is characterised in that:Described buffer cell by Buffer Pool with
And its internal cushioning liquid composition, lower adsorptive pads and buffer solution contact are contained in, Buffer Pool can be inserted into upper shell, lower house
Patchhole on the downside of the box body of composition.
According to quick blood typing detection card as described above, it is characterised in that:Coating in described sample pad is set
There are anti-A, B or Rh antibody and preservative.
According to quick blood typing detection card as described above, it is characterised in that:Also include sampling unit;Described sampling
Unit is made up of capillary and rubber suction nozzle, and capillary is arranged on the side of rubber suction nozzle.
According to quick blood typing detection card as described above, it is characterised in that:Described capillary by hydrophilic plastic or
Person's glass material is constituted.
According to quick blood typing detection card as described above, it is characterised in that:Described capillary diameter of inner pipe is
1.35 ± 0.1mm, every 5 microlitres of scales.
According to quick blood typing detection card as described above, it is characterised in that:Described rubber suction nozzle uses rubber material
Material composition, its diameter of inner pipe is identical with capillary diameter.
According to quick blood typing detection card as described above, it is characterised in that:Also include matching used with blood type card
Detector;Described detector is made up of optically detecting unit, image-signal processor and electronics peripheral hardware, is read by optical detection
Take blood typing detection card result.
Technique effect of the invention is embodied in:The present invention realizes the fast of blood group from user's angle by solid phase method
Speed sizing test.It is bold by reagent and combinations of getting stuck, make whole detection integration, not only convenient use person's carrying, while whole
Easy to operate, the clean hygiene of individual detection.Only need 5 microlitres of blood sample, naked eyes can at 10 seconds or so quick interpretation bleeding type,
For emergency judges that blood group has saved the time.The detection card of blood sample can will be also added to be put into matched inspection simultaneously
Surveying in device carries out interpretation, and prints and carry out staying shelves to preserve, it is convenient after it is for future reference.The present invention meets each field to blood well
The demand of type fast shaping.
Brief description of the drawings
Fig. 1 is abo blood group of the present invention sizing card front view;
Fig. 2 is abo blood group of the present invention sizing card rearview;
Fig. 3 is ABO and RhD blood typings card front view of the present invention;
Fig. 4 is ABO and RhD blood typings card rearview of the present invention;
Fig. 5 is side structure schematic diagram of the present invention;
Fig. 6 is sampling unit schematic diagram;
Fig. 7 is and the matching used detector schematic diagram of blood type card;
Fig. 8 is abo blood group sizing card upper shell schematic diagram;
Fig. 9 is abo blood group sizing card lower house schematic diagram;
Figure 10 is ABO and RhD blood typing card upper shell schematic diagrames;
Figure 11 is ABO and RhD blood typing card lower house schematic diagrames;
Figure 12 is buffer cell schematic diagram;
Figure 13 is detection reagent bar schematic diagram.
Description of reference numerals:Upper shell 1, upper adsorptive pads 2, lower house 3, well 4, sample pad 5, lower adsorptive pads 6, buffering
Pond 7, rubber suction nozzle 8, capillary 9, optically detecting unit 10.
Specific embodiment
The present invention is done below in conjunction with the accompanying drawings further is described in detail.
Quick blood typing detection card of the invention, employs the fast shaping that solid phase method realizes blood group, including sizing inspection
Survey unit and buffer cell, while also include sampling unit and with the matching used detector of blood type card.As shown in fig. 6, this hair
Bright sampling unit, is made up of capillary 9 and rubber suction nozzle 8, using capillarity, quantitative collection trace sample.In the present invention,
Capillary 9 is preferably made up of hydrophilic plastic or glass material, and its diameter of inner pipe is 1.35 ± 0.1mm, an every 5 microlitres quarters
Degree, because only needing 5 microlitres per hole sample size, can so reduce the collection capacity of sample, and rubber suction nozzle 8 uses elastomeric material
Composition, its diameter of inner pipe is identical with capillary diameter, and capillary can so be intimately associated with rubber suction nozzle, makes blood sampling more
Plus it is convenient.
As shown in Fig. 1 to Fig. 5, Fig. 8 to Figure 13, it is of the invention sizing detection unit by upper shell 1, with upper shell clamping
Lower house 3 and detection reagent bar are constituted, and wherein lower house 3 is provided with locating slot, and locating slot is fixedly mounted on for detection reagent bar
On lower house 3.And upper shell 1, lower house 3 are used for common carrying detection reagent bar, detection reagent bar is by sample pad 5, upper water suction
Pad 2 and lower adsorptive pads 6 are constituted.Lower adsorptive pads 6 are acted on by chromatography, and by buffer solution water conservancy diversion to sample pad 5, upper adsorptive pads 2 are same
Sample is acted on by chromatography, by unreacted blood sample in sample pad 5 and the cushioning liquid water conservancy diversion by coming on the water conservancy diversion of lower adsorptive pads 6
To in upper adsorptive pads 2.Coating in inventive samples pad 5 is provided with anti-A, B or Rh antibody and its preservative, for blood typing
Reaction, sample pad 5 faces setting well 4 on upper shell 1, is easy to sample-adding.The box of upper shell of the present invention 1, the composition of lower house 3
Body downside is provided with patchhole, and Buffer Pool 7 can be inserted into the patchhole, and after insertion, Buffer Pool 7 is contacted with lower adsorptive pads 6.The present invention is slow
Rush pond 7 and be contained in its internal cushioning liquid composition buffer cell.
If Fig. 7 is the matching used detector of blood type card, it is by optically detecting unit 10, image-signal processor and correlation
Electronics peripheral hardware is constituted, and is read blood typing by optical detection and is detected card result, including on LCD screen, and print
Come.
The course of work of the invention is:When there is corresponding reaction time of blood coagulation, blood sample cannot be rinsed dry by cushioning liquid
Only, the aobvious red of sample pad 5;Generation reaction time of blood coagulation, sample pad 5 shows colourless.Buffer cell is by 7 groups of cushioning liquid and Buffer Pool
Into as long as buffer concentration is of substantially equal with human body cell liquid, you can keep the concentration of intraor extracellular equal, also will not
Cell is risen brokenly or shrinkage, lead to not observation.Cushioning liquid is used for the detection reacted flushing of card of shaping, and Buffer Pool 7 is used for
Carry cushioning liquid;With the matching used detector of blood type card by optically detecting unit 10, image-signal processor and mutually powered-down
Sub- peripheral hardware composition, reads blood typing and detects card result by optical detection, including on LCD screen, and print
Come.The present invention using solid phase method not only detect quickly, reagent and sample consumption it is few, while integrated design is easy to carry, grasps
Make convenient, clean hygiene.A large amount of blood source shaping mechanisms such as blood station, hospital are applicable not only to, while being also applied for family, individuality
Deng.
Example 1:Abo blood group is detected.
Operating procedure:
1st, blood type test card is placed horizontally on dry workbench;
2nd, with 10 microlitres of sampling unit fetching blood sample(At second graduation mark), respectively to 5 microlitres of samples of addition in two wells 4
This;
3rd, gently pick up blood type test card, during Buffer Pool 7 is firmly pushed housing by hand slightly, and detection card is vertically placed on work
On platform(Buffer Pool one end is downward), stand 10 seconds or so, can naked eyes interpretation sizing result, blood type card can be also put into supporting with it
In the detector draw-in groove for using, interpretation sizing result.
As it will be easily appreciated by one skilled in the art that the foregoing is only presently preferred embodiments of the present invention, it is not used to
The limitation present invention, all any modification, equivalent and improvement made within the spirit and principles in the present invention etc., all should include
Within protection scope of the present invention.
Claims (8)
1. a kind of quick blood typing detection blocks, including sizing detection unit and buffer cell, it is characterised in that:Described sizing
Detection unit is made up of upper shell, lower house and detection reagent bar, and upper shell is provided with well, and lower house is provided with locating slot,
Locating slot is used to for detection reagent bar to be fixedly mounted on lower house, described detection reagent bar by sample pad, upper adsorptive pads and
Lower adsorptive pads composition, sample pad is arranged between adsorptive pads and lower adsorptive pads;Sample pad faces well, upper shell, under
The box body downside of housing composition is provided with patchhole, and buffer cell is arranged in the hole, and lower adsorptive pads are contacted with buffer cell.
2. quick blood typing detection according to claim 1 blocks, it is characterised in that:Described buffer cell is by Buffer Pool
And its internal cushioning liquid composition, lower adsorptive pads and buffer solution contact are contained in, Buffer Pool can be inserted into upper shell, lower casing
Patchhole on the downside of the box body of body composition.
3. quick blood typing detection according to claim 1 and 2 blocks, it is characterised in that:Bag in described sample pad
It is equipped with anti-A, B or Rh antibody and preservative.
4. quick blood typing detection according to claim 1 and 2 blocks, it is characterised in that:Also include sampling unit;It is described
Sampling unit be made up of capillary and rubber suction nozzle, capillary is arranged on the side of rubber suction nozzle.
5. quick blood typing detection according to claim 4 blocks, it is characterised in that:Described capillary is by hydrophilic plastic
Or glass material composition.
6. quick blood typing detection according to claim 4 blocks, it is characterised in that:Described capillary diameter of inner pipe is
1.35 ± 0.1mm, every 5 microlitres of scales.
7. quick blood typing detection according to claim 4 blocks, it is characterised in that:Described rubber suction nozzle uses rubber
Material is constituted, and its diameter of inner pipe is identical with capillary diameter.
8. quick blood typing detection according to claim 1 blocks, it is characterised in that:Also include being supported the use with blood type card
Detector;Described detector is made up of optically detecting unit, image-signal processor and electronics peripheral hardware, by optical detection
Read blood typing detection card result.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201611243101.7A CN106771267A (en) | 2016-12-29 | 2016-12-29 | Quick blood type design detects card |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201611243101.7A CN106771267A (en) | 2016-12-29 | 2016-12-29 | Quick blood type design detects card |
Publications (1)
Publication Number | Publication Date |
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CN106771267A true CN106771267A (en) | 2017-05-31 |
Family
ID=58923680
Family Applications (1)
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CN201611243101.7A Pending CN106771267A (en) | 2016-12-29 | 2016-12-29 | Quick blood type design detects card |
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Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2250342A (en) * | 1990-11-27 | 1992-06-03 | Pall Corp | Blood typing |
CN1894584A (en) * | 2003-07-09 | 2007-01-10 | 麦迪奥诊断产品有限公司 | Device and method for simultaneously carrying out blood group determination, serum cross-check and antibody detection test |
WO2009149615A1 (en) * | 2008-06-10 | 2009-12-17 | 英科新创(厦门)科技有限公司 | A method of quickly determining human abo/rh/mn blood type and a test kit thereof |
US20100323343A1 (en) * | 2009-05-11 | 2010-12-23 | Nexus Dx, Inc. | Methods and compositions for analyte detection |
CN103502819A (en) * | 2011-04-21 | 2014-01-08 | 西门子公司 | Blood typing devices and methods for testing blood type |
CN205027765U (en) * | 2015-09-23 | 2016-02-10 | 英科新创(厦门)科技有限公司 | Positive and negative design comprehensive testing card of blood ABO and rhD |
CN206369741U (en) * | 2016-12-29 | 2017-08-01 | 武汉伽诺美科技有限公司 | Quick blood type design detects card |
-
2016
- 2016-12-29 CN CN201611243101.7A patent/CN106771267A/en active Pending
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2250342A (en) * | 1990-11-27 | 1992-06-03 | Pall Corp | Blood typing |
CN1894584A (en) * | 2003-07-09 | 2007-01-10 | 麦迪奥诊断产品有限公司 | Device and method for simultaneously carrying out blood group determination, serum cross-check and antibody detection test |
WO2009149615A1 (en) * | 2008-06-10 | 2009-12-17 | 英科新创(厦门)科技有限公司 | A method of quickly determining human abo/rh/mn blood type and a test kit thereof |
US20100323343A1 (en) * | 2009-05-11 | 2010-12-23 | Nexus Dx, Inc. | Methods and compositions for analyte detection |
CN103502819A (en) * | 2011-04-21 | 2014-01-08 | 西门子公司 | Blood typing devices and methods for testing blood type |
CN205027765U (en) * | 2015-09-23 | 2016-02-10 | 英科新创(厦门)科技有限公司 | Positive and negative design comprehensive testing card of blood ABO and rhD |
CN206369741U (en) * | 2016-12-29 | 2017-08-01 | 武汉伽诺美科技有限公司 | Quick blood type design detects card |
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Effective date of registration: 20171116 Address after: 436070 Hubei Ezhou city Gedian Development Zone Construction Avenue 216 East Lake high new wisdom city 24-2-202 room Applicant after: Hubei Ganuo beauty Biotechnology Co. Ltd. Address before: 430074 Wuhan hi-tech Avenue, Wuhan, Hubei Province, bio City Innovation Park, C6 Dong Bei Lou 402 Applicant before: Wuhan Ganuo beauty Technology Co. Ltd. |
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Application publication date: 20170531 |