CN106770030B - Pesticide residue detection device based on point target frequency spectrum detection - Google Patents
Pesticide residue detection device based on point target frequency spectrum detection Download PDFInfo
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- CN106770030B CN106770030B CN201611091982.5A CN201611091982A CN106770030B CN 106770030 B CN106770030 B CN 106770030B CN 201611091982 A CN201611091982 A CN 201611091982A CN 106770030 B CN106770030 B CN 106770030B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
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Abstract
The present invention is based on the pesticide residue detection devices of point target frequency spectrum detection to belong to food safety, Pesticides Testing technical field；The device is sequentially placed containing liquid chamber, pinhole array plate, fourier lense and imaging sensor in the camera bellows that inner surface is provided with black flannelette；Containing liquid chamber is placed relative to inclined light shaft, it is provided on the outside of containing liquid chamber to perpendicular to optical axial plane and is projected as circular hole, the directional light parallel with optical axis direction is irradiated from side outside hole into containing liquid chamber, the transparent adjustment plate that can be moved is provided on the inside of containing liquid chamber, for the transparent adjustment plate tool there are two mobile position, containing liquid chamber inside is equipped with pesticide residue extracting solution；The pin hole of multiple arrangements of direction in length and breadth is distributed on pinhole array plate；Fourier lense is perpendicular to optical axis direction fixed placement, and pinhole array plate is located at the first position of focal plane of fourier lense；Imaging sensor is located at the second position of focal plane of fourier lense；The present invention provides a kind of completely new the Detection Technologies of Pesticide Residues means.
Description
Technical field
The present invention is based on the pesticide residue detection devices of point target frequency spectrum detection to belong to food safety, Pesticides Testing technology neck
Domain.
Background technique
The development of agriculture industrialization makes the production of agricultural product be increasingly dependent on the allogenic materials such as pesticide.China's pesticide is in agriculture
The dosage of product is high, and the unreasonable use of these substances will lead to the excessive pesticide residues in agricultural product, influences
Consumer's edible safety will cause consumer and cause a disease, develop abnormal, or even directly results in and be poisoned to death when serious.Pesticide is residual
The exceeded trade that also will affect agricultural product is stayed, China's Agricultural outlet is made to be faced with formidable challenges.No matter control persticide residue
For food safety, or for national image, the primary method for being all of great significance, and controlling pesticide residue is exactly to carry out
Detecting Pesticide.
Traditional Pesticides Testing method is GC/MS detection method, but the farming residual analysis technology testing cost such as traditional GC/MS
Height, time are long, bring many inconvenience to the supervision work before, during and after the production process to agricultural product of food safety Regulation department, this
A problem expedites the emergence of out the detection technique of a large amount of quickly pesticide residue, common are chemical fast determining method, immunoassay, enzyme and inhibits
Method and In vivo detection method etc..
Chemical fast determining method mainly changes colour according to redox reaction, hydrolysate and detection liquid effect, is used for organic phosphorus agriculture
The quick detection of medicine, but sensitivity is low, interferes using limitation, and vulnerable to reducing substances.
Immunoassay mainly has radiommunoassay and EIA enzyme immunoassay, and the most commonly used is ELISAs
(ELISA), specific recognition and association reaction based on antigen and antibody need to prepare artificial anti-for small-molecular-weight pesticide
Original just can be carried out immunoassay.
Enzyme inhibition is to study most mature, the most widely used quick residual detection technique of agriculture, mainly according to organic phosphorus and ammonia
Carbamate class pesticide inhibits reaction to the specificity of enzyme acetylcholine.
In vivo detection method, it is main to utilize living body biological to the sensitivity response of pesticide residue, such as housefly feeding sample is given, it sees
The death rate is examined to determine agriculture residual.This method is easy to operate, but it is qualitative it is coarse, accuracy is low, to the narrow application range of pesticide.
From the point of view of the developing history of the Detection Technologies of Pesticide Residues, with being constantly progressive for technological means, also have more new
Method occurs.
Summary of the invention
In order to detect pesticide residue, the invention discloses a kind of, and the Detecting Pesticide based on point target frequency spectrum detection is filled
It sets, provides a kind of completely new the Detection Technologies of Pesticide Residues means.
The object of the present invention is achieved like this:
Based on the pesticide residue detection device of point target frequency spectrum detection, in the camera bellows that inner surface is provided with black flannelette according to
Secondary placement containing liquid chamber, pinhole array plate, fourier lense and imaging sensor；
The containing liquid chamber is placed relative to inclined light shaft, is provided on the outside of containing liquid chamber to perpendicular to optical axial plane and is projected as
Circular hole, the directional light parallel with optical axis direction are irradiated from side outside hole into containing liquid chamber, and being provided on the inside of containing liquid chamber can
Mobile transparent adjustment plate, for the transparent adjustment plate tool there are two mobile position, containing liquid chamber inside is equipped with pesticide residue extracting solution；
Multiple rows of directions in length and breadth are distributed with perpendicular to optical axis direction fixed placement in the pinhole array plate on pinhole array plate
The pin hole of column, the distance between two neighboring pin hole is equal, and is not less than hole to the diameter projected perpendicular to optical axial plane；
The fourier lense is perpendicular to optical axis direction fixed placement, and pinhole array plate is located at the first of fourier lense
Position of focal plane；
Described image sensor is located at the second position of focal plane of fourier lense perpendicular to optical axis direction fixed placement；
The directional light parallel with optical axis direction is irradiated from side outside hole into containing liquid chamber, due to the work by pesticide residue extracting solution
With reflecting, the light beam being emitted from containing liquid chamber is relative to incident parallel light there are the directional light of lateral displacement, and outgoing is parallel
Light illuminates a pin hole in pinhole array plate, and the pin hole being illuminated and imaging sensor are null tone relationship, and then in image
The frequency spectrum of pin hole is obtained on sensor.
It is a kind of realized on the above-mentioned pesticide residue detection device based on point target frequency spectrum detection based on point target frequency spectrum
The pesticide residue detection method of detection, comprising the following steps:
Step a, pesticide residue is extracted with transparent solvent, being formed by solute, transparent solvent of pesticide is the solution of solvent；
Step b, the obtained solution of step a is poured into containing liquid chamber；
Step c, it is irradiated from side outside hole into containing liquid chamber with the directional light parallel with optical axis direction；
Step d, whether pin hole is lit with imaging sensor observation, if:
There is pin hole to be lit, the transparent adjustment plate in containing liquid chamber keeps current mobile position, enters step e；
It is pin-free to be lit, the transparent adjustment plate in containing liquid chamber is adjusted to another movement position, makes have pin hole to be lit,
Enter step e；
Step e, the size of hot spot or/and position on imaging sensor are obtained；
Step f, the size of hot spot or/and position on the imaging sensor obtained according to step e, judge to be lit is needle
Which pin hole on hole array plate；
Step g, according to the pin hole coordinate being lit, judge transverse direction of the containing liquid chamber outgoing directional light relative to incident parallel light
Displacement；
Step h, the lateral displacement obtained according to step g obtains solution in conjunction with the mobile position of adjustment plate transparent in containing liquid chamber
Refractive index；
Step i, according to the refractive index of solution, persticide residue is obtained.
The above-mentioned pesticide residue detection method based on point target frequency spectrum detection,
The size of hot spot described in step f or/and position be lit be which pin hole on pinhole array plate it
Between corresponding relationship, obtained by the method for calibration；
Corresponding relationship between pin hole coordinate and lateral displacement described in step g is obtained by the method for calibration；
Corresponding relationship described in step h between lateral displacement and refractive index is obtained by the method for calibration；
Corresponding relationship described in step i between refractive index and persticide residue is obtained by the method for calibration.
The utility model has the advantages that
The first, present invention finds when the pesticide solution concentration difference, the characteristic of solution refractive index can be changed, invented one kind
Pesticide residue detection device and detection method based on point target frequency spectrum detection provide a kind of completely new Detecting Pesticide skill
Art means.
The second, in apparatus of the present invention, it is provided with black flannelette using camera bellows, and in camera bellows inner surface, it can be to camera bellows
Interior stray light is absorbed, and noise jamming is reduced.
Third, in apparatus of the present invention, containing liquid chamber comes down in torrents placement relative to optical axis, is to realize that lateral position occurs for directional light
Key Design, establish key technology for Detecting Pesticide.
4th, in apparatus of the present invention, transparent adjustment plate tool there are two mobile position, is avoided in specific refractive index situation
Under, the directional light being emitted from containing liquid chamber is got to just between two pin holes of pinhole array plate.
5th, the present invention only needs to light directional light and most successive steps transparent adjustment Board position when operation
Operation, operating process very simple, layman can also operate, and considerably increase the scope of application of device.
6th, after calibration, the size of hot spot or/and position can directly be obtained the present invention by computer vision technique
, and persticide residue can be directly obtained by the size or/and position of hot spot, all intermediate computations links are saved, equally
Layman can operate, and considerably increase the scope of application of device.
Detailed description of the invention
Fig. 1 is that the present invention is based on the structural schematic diagrams of the pesticide residue detection device of point target frequency spectrum detection.
In figure: 1 containing liquid chamber, 2 pinhole array plates, 3 fourier lenses, 4 imaging sensors.
Specific embodiment
Specific embodiment one
The present embodiment is the embodiment of the pesticide residue detection device based on point target frequency spectrum detection.
The pesticide residue detection device based on point target frequency spectrum detection of the present embodiment, structural schematic diagram was as shown in Figure 1, should
Detection device is sequentially placed containing liquid chamber 1, pinhole array plate 2, fourier lense 3 in the camera bellows that inner surface is provided with black flannelette
With imaging sensor 4；
The containing liquid chamber 1 is placed relative to inclined light shaft, and the outside of containing liquid chamber 1, which is provided with to perpendicular to optical axial plane, to be projected
For circular hole, the directional light parallel with optical axis direction is irradiated from side outside hole into containing liquid chamber 1, and the inside of containing liquid chamber 1 is provided with
The transparent adjustment plate that can be moved, the transparent adjustment plate tool extract inside containing liquid chamber 1 equipped with pesticide residue there are two mobile position
Liquid；
Multiple directions in length and breadth are distributed on pinhole array plate 2 perpendicular to optical axis direction fixed placement in the pinhole array plate 2
The pin hole of arrangement, the distance between two neighboring pin hole is equal, and is not less than hole to the diameter projected perpendicular to optical axial plane；
The fourier lense 3 is perpendicular to optical axis direction fixed placement, and pinhole array plate 2 is located at fourier lense 3
First position of focal plane；
Described image sensor 4 is located at the second focal plane position of fourier lense 3 perpendicular to optical axis direction fixed placement
It sets；
The directional light parallel with optical axis direction is irradiated from side outside hole into containing liquid chamber 1, due to by pesticide residue extracting solution
Effect reflects, and the light beam being emitted from containing liquid chamber 1 is relative to incident parallel light there are the directional light of lateral displacement, and outgoing is flat
Row light illuminates a pin hole in pinhole array plate 2, and the pin hole being illuminated and imaging sensor 4 are null tone relationship, Jin Er
The frequency spectrum of pin hole is obtained on imaging sensor 4.
Specific embodiment two
The present embodiment is the embodiment of the pesticide residue detection method based on point target frequency spectrum detection.
The pesticide residue detection method based on point target frequency spectrum detection of the present embodiment is based on described in the specific embodiment one
It is realized on the pesticide residue detection device of point target frequency spectrum detection, it should the Detecting Pesticide side based on point target frequency spectrum detection
Method, comprising the following steps:
Step a, pesticide residue is extracted with transparent solvent, being formed by solute, transparent solvent of pesticide is the solution of solvent；
This step is it should be noted that the application is detected just for the pesticide with following characteristic: can be dissolved in
Bright solvent, the pesticide dissolved by transparent solvent；Pesticide concentration changes the pesticide that can change solution refractive index；In this step, have
The extracting method of body, when the determinand that those skilled in the art can choose fixed mass impregnates fixed in fixed volume solvent
Between extract, and the quality of determinand, the volume of solvent, the selections of the parameters such as soaking time, those skilled in the art are logical
Crossing limited trials just can determine, and specific parameter, and not be a unique amount, as long as being marked in subsequent steps
It is fixed, it is all feasible；
It is extracted using which type of solvent, Rational choice is carried out according to the type of pesticide, this chooses work, right
It is also to be easily achieved for those skilled in the art, in this application without being described in detail；
Step b, the obtained solution of step a is poured into containing liquid chamber 1；
Step c, it is irradiated from side outside hole into containing liquid chamber 1 with the directional light parallel with optical axis direction；
Step d, whether pin hole is lit with the observation of imaging sensor 4, if:
There is pin hole to be lit, the transparent adjustment plate in containing liquid chamber 1 keeps current mobile position, enters step e；
It is pin-free to be lit, the transparent adjustment plate in containing liquid chamber 1 is adjusted to another movement position, makes have pin hole by point
It is bright, enter step e；
Step e, the size of hot spot or/and position on imaging sensor 4 are obtained；
Step f, the size of hot spot or/and position on the imaging sensor 4 obtained according to step e, judge to be lit is
Which pin hole on pinhole array plate 2；
Step g, according to the pin hole coordinate being lit, judge that containing liquid chamber 1 is emitted cross of the directional light relative to incident parallel light
To displacement；
Step h, the lateral displacement obtained according to step g obtains molten in conjunction with the mobile position of transparent adjustment plate in containing liquid chamber 1
The refractive index of liquid；
Step i, according to the refractive index of solution, persticide residue is obtained.
Specific embodiment three
The present embodiment is the embodiment of the pesticide residue detection method based on point target frequency spectrum detection.
The pesticide residue detection method based on point target frequency spectrum detection of the present embodiment, on the basis of specific embodiment two
It further defines that
The size of hot spot described in step f or/and position be lit be which pin hole on pinhole array plate 2 it
Between corresponding relationship, obtained by the method for calibration；
Corresponding relationship between pin hole coordinate and lateral displacement described in step g is obtained by the method for calibration；
Corresponding relationship described in step h between lateral displacement and refractive index is obtained by the method for calibration；
Corresponding relationship described in step i between refractive index and persticide residue is obtained by the method for calibration.
It should be noted that the specific calibration numerical value in specific embodiment two and specific embodiment three, it be according to pesticide
The design parameter of each element could finally confirm in type, the application device, as long as scaling method can define, art technology
Personnel can obtain calibration numerical value, due to it is provided by the present application be a kind of technological means, rather than a set of specific technology ginseng
Number, therefore specific calibration numerical value is not illustrated in this application；And specific scaling method, and belong to the routine of this field
Technological means, the time and the calibration experiment for putting position that dotted timer obtains when those skilled in the art expect senior middle school, just
Being able to know that the application calibration experiment, how this carries out, therefore does not also need to be described in detail in this application.
Claims (1)
1. the pesticide residue detection device based on point target frequency spectrum detection, which is characterized in that inner surface is provided with black flannelette
Camera bellows in be sequentially placed containing liquid chamber (1), pinhole array plate (2), fourier lense (3) and imaging sensor (4)；
The containing liquid chamber (1) is placed relative to inclined light shaft, is provided on the outside of containing liquid chamber (1) to perpendicular to optical axial plane and is projected
For circular hole, from side outside hole to the middle irradiation of containing liquid chamber (1), containing liquid chamber is set the directional light parallel with optical axis direction on the inside of (1)
It is equipped with the transparent adjustment plate that can be moved, for the transparent adjustment plate tool there are two mobile position, containing liquid chamber (1) is internal residual equipped with pesticide
Stay extracting solution；
Multiple directions in length and breadth are distributed on pinhole array plate (2) perpendicular to optical axis direction fixed placement in the pinhole array plate (2)
The pin hole of arrangement, the distance between two neighboring pin hole is equal, and is not less than hole to the diameter projected perpendicular to optical axial plane；
The fourier lense (3) is perpendicular to optical axis direction fixed placement, and pinhole array plate (2) is located at fourier lense (3)
The first position of focal plane；
Described image sensor (4) is located at the second focal plane position of fourier lense (3) perpendicular to optical axis direction fixed placement
It sets；
The directional light parallel with optical axis direction is from side outside hole to the middle irradiation of containing liquid chamber (1), due to the work by pesticide residue extracting solution
With reflecting, the light beam being emitted from containing liquid chamber (1) is relative to incident parallel light there are the directional light of lateral displacement, and outgoing is flat
Row light illuminates a pin hole in pinhole array plate (2), and the pin hole being illuminated and imaging sensor (4) they are null tone relationship, into
And the frequency spectrum of pin hole is obtained on imaging sensor (4)；
The pesticide residue detection method realized on the above-mentioned pesticide residue detection device based on point target frequency spectrum detection, including with
Lower step:
Step a, pesticide residue is extracted with transparent solvent, being formed by solute, transparent solvent of pesticide is the solution of solvent；
Step b, the obtained solution of step a is poured into containing liquid chamber (1)；
Step c, it is irradiated with the directional light parallel with optical axis direction from side outside hole to containing liquid chamber (1) is middle；
Step d, whether pin hole is lit with imaging sensor (4) observation, if:
There is pin hole to be lit, the transparent adjustment plate in containing liquid chamber (1) keeps current mobile position, enters step e；
It is pin-free to be lit, the transparent adjustment plate in containing liquid chamber (1) is adjusted to another movement position, makes have pin hole to be lit,
Enter step e；
Step e, the size of hot spot or/and position on imaging sensor (4) are obtained；
Step f, the size of hot spot or/and position on the imaging sensor (4) obtained according to step e, judge to be lit is needle
Which pin hole on hole array plate (2)；
Step g, according to the pin hole coordinate being lit, judge transverse direction of containing liquid chamber (1) outgoing directional light relative to incident parallel light
Displacement；
Step h, the lateral displacement obtained according to step g obtains solution in conjunction with the mobile position of transparent adjustment plate in containing liquid chamber (1)
Refractive index；
Step i, according to the refractive index of solution, persticide residue is obtained.
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| CN201611091982.5A CN106770030B (en) | 2016-12-01 | 2016-12-01 | Pesticide residue detection device based on point target frequency spectrum detection |
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| CN201611091982.5A CN106770030B (en) | 2016-12-01 | 2016-12-01 | Pesticide residue detection device based on point target frequency spectrum detection |
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| CN106770030B true CN106770030B (en) | 2019-07-19 |
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| CN101995393A (en) * | 2010-09-30 | 2011-03-30 | 哈尔滨工程大学 | Device and method for measuring concentration of liquid based on glass synthetic body and linear array charge-coupled device (CCD) |
| CN103308142A (en) * | 2013-05-28 | 2013-09-18 | 华南师范大学 | Method and device for measuring speed and frequency of ultrasonic traveling wave in liquid |
| CN103645119A (en) * | 2013-12-17 | 2014-03-19 | 哈尔滨工程大学 | Point light source-based measuring device for measuring liquid density of matrix CCD (Charged Coupled Device) |
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2016
- 2016-12-01 CN CN201611091982.5A patent/CN106770030B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1601244A (en) * | 2003-09-22 | 2005-03-30 | 奥林巴斯株式会社 | Focus detecting unit, and refractive index measuring apparatus and non-contact thermometer using the same |
| EP1645864A3 (en) * | 2004-10-07 | 2006-05-24 | Wyatt Technology Corporation | Enhanced sensitivity differential refractometer incorporating a photodetector array |
| CN1936543A (en) * | 2006-10-10 | 2007-03-28 | 中国海洋大学 | Apparatus and method for measuring organic maturity degree of hydrocarbon source rocks |
| CN101995393A (en) * | 2010-09-30 | 2011-03-30 | 哈尔滨工程大学 | Device and method for measuring concentration of liquid based on glass synthetic body and linear array charge-coupled device (CCD) |
| CN103308142A (en) * | 2013-05-28 | 2013-09-18 | 华南师范大学 | Method and device for measuring speed and frequency of ultrasonic traveling wave in liquid |
| CN103645119A (en) * | 2013-12-17 | 2014-03-19 | 哈尔滨工程大学 | Point light source-based measuring device for measuring liquid density of matrix CCD (Charged Coupled Device) |
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Effective date of registration: 20221208 Address after: F11-13, Building B, No.1 R&D 1st Road, Songshanhu Hi tech Industrial Development Zone, Dongguan, Guangdong 523000 Patentee after: Dongguan Huace Testing&Certification Co.,Ltd. Patentee after: CENTRE TESTING INTERNATIONAL GROUP Co.,Ltd. Address before: 518101 building 101, No. 1, huatest building, Xingdong community, Xin'an street, Bao'an District, Shenzhen, Guangdong Province Patentee before: CENTRE TESTING INTERNATIONAL GROUP Co.,Ltd. |