CN106770030A - Pesticide residue detection device based on point target frequency spectrum detection - Google Patents
Pesticide residue detection device based on point target frequency spectrum detection Download PDFInfo
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- CN106770030A CN106770030A CN201611091982.5A CN201611091982A CN106770030A CN 106770030 A CN106770030 A CN 106770030A CN 201611091982 A CN201611091982 A CN 201611091982A CN 106770030 A CN106770030 A CN 106770030A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
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Abstract
Pesticide residue detection device of the present invention based on point target frequency spectrum detection belongs to food security, Pesticides Testing technical field；The device is sequentially placed containing liquid chamber, pinhole array plate, fourier lense and imageing sensor in inner surface setting has the camera bellows of black flannelette；Containing liquid chamber is placed relative to inclined light shaft, the outside of containing liquid chamber sets the oriented hole that circle is projected as perpendicular to optical axial plane, the directional light parallel with optical axis direction irradiates from lateral containing liquid chamber outside hole, the transparent adjustment plate of movement is provided with the inside of containing liquid chamber, the transparent adjustment plate has two mobile positions, and containing liquid chamber inside is equipped with residues of pesticides extract solution；The pin hole of multiple arrangements of direction in length and breadth is distributed with pinhole array plate；Fourier lense is perpendicular to optical axis direction fixed placement, and pinhole array plate is located at the first position of focal plane of fourier lense；Imageing sensor is located at the second position of focal plane of fourier lense；The invention provides a kind of brand-new the Detection Technologies of Pesticide Residues means.
Description
Technical field
Pesticide residue detection device of the present invention based on point target frequency spectrum detection belongs to food security, Pesticides Testing technology neck
Domain.
Background technology
The development of agriculture industrialization makes the production of agricultural product be increasingly dependent on the allogenic materials such as agricultural chemicals.China's agricultural chemicals is in agriculture
The consumption of product remains high, and the unreasonable use of these materials will cause the residues of pesticides in agricultural product exceeded, influence
Consumer's edible safety, can cause consumer to cause a disease, develop abnormal when serious, or even directly results in and be poisoned to death.Agricultural chemicals is residual
The exceeded trade that can also influence agricultural product is stayed, China's Agricultural is exported and is faced with formidable challenges.Control persticide residue, no matter
For food security, or for national image, all it is significant, and it is exactly to carry out to control the primary method of residues of pesticides
Detecting Pesticide.
Traditional Pesticides Testing method is GC/MS detection methods, but the farming residual analysis technology for detection cost such as traditional GC/MS
The high, time is long, and many inconvenience are brought to the supervision work before, during and after the production process to agricultural product of food safety Regulation department, this
Individual problem expedites the emergence of out the detection technique of substantial amounts of quick residues of pesticides, common are chemical fast determining method, immunoassay, enzyme level
Method and In vivo detection method etc..
Chemical fast determining method, mainly according to redox reaction, hydrolysate changes colour with detection liquid effect, for organophosphor agriculture
The quick detection of medicine, but sensitivity is low, using limitation, and is easily disturbed by reducing substances.
Immunoassay, mainly there is radiommunoassay and EIA enzyme immunoassay, most commonly ELISA
（ELISA）, specific recognition and association reaction based on antigen and antibody need to prepare artificial resisting for small-molecular-weight agricultural chemicals
Original, can just carry out immunoassay.
Inhibiting AChE, is to study most ripe, the most widely used quick residual detection technique of agriculture, mainly according to organophosphor and ammonia
Carbamate class agricultural chemicals suppresses reaction to the specificity of enzyme acetylcholine.
In vivo detection method, it is main to utilize living body biological to the sensitivity response of residues of pesticides, housefly feeding sample is for example given, see
The death rate is examined to judge agriculture residual.The method is simple to operate, but the qualitative coarse, degree of accuracy is low, to the narrow application range of agricultural chemicals.
From the point of view of the developing history of the Detection Technologies of Pesticide Residues, with the continuous progress of technological means, also have more new
Method occurs.
The content of the invention
In order to detect residues of pesticides, the invention discloses a kind of Detecting Pesticide dress based on point target frequency spectrum detection
Put, there is provided a kind of brand-new the Detection Technologies of Pesticide Residues means.
The object of the present invention is achieved like this：
Based on the pesticide residue detection device of point target frequency spectrum detection, put successively in inner surface setting has the camera bellows of black flannelette
Put containing liquid chamber, pinhole array plate, fourier lense and imageing sensor；
The containing liquid chamber is placed relative to inclined light shaft, and the outside setting of containing liquid chamber is oriented to be projected as circle perpendicular to optical axial plane
Hole, the directional light parallel with optical axis direction irradiated from lateral containing liquid chamber outside hole, and movement is provided with the inside of containing liquid chamber
Transparent adjustment plate, the transparent adjustment plate has two mobile positions, and containing liquid chamber inside is equipped with residues of pesticides extract solution；
The pinhole array plate is distributed with multiple arrangements of direction in length and breadth in optical axis direction fixed placement, pinhole array plate
The distance between pin hole, two neighboring pin hole are equal, and not less than hole to the diameter projected perpendicular to optical axial plane；
The fourier lense is perpendicular to optical axis direction fixed placement, and pinhole array plate is located at the first focal plane of fourier lense
Position；
Described image sensor perpendicular to optical axis direction fixed placement, and positioned at the second position of focal plane of fourier lense；
The directional light parallel with optical axis direction irradiates from lateral containing liquid chamber outside hole, because the effect by residues of pesticides extract solution is sent out
Raw refraction, the light beam from containing liquid chamber outgoing is the directional light that there is lateral displacement relative to incident parallel light, and outgoing directional light will
A pin hole in pinhole array plate illuminates, and the pin hole being illuminated is null tone relation with imageing sensor, and then in image sensing
The frequency spectrum of pin hole is obtained on device.
It is a kind of on the above-mentioned pesticide residue detection device based on point target frequency spectrum detection realize based on point target frequency spectrum
The pesticide residue detection method of detection, comprises the following steps：
Step a, with transparent solvent extract residues of pesticides, formed with agricultural chemicals as solute, solution of the transparent solvent as solvent；
Step b, the solution that step a is obtained is poured into containing liquid chamber；
Step c, irradiated from lateral containing liquid chamber outside hole with the directional light parallel with optical axis direction；
Step d, with imageing sensor observe whether there is pin hole to be lit, if：
There is pin hole to be lit, the transparent adjustment plate in containing liquid chamber keeps current mobile position, into step e；
It is pin-free to be lit, the transparent adjustment plate in containing liquid chamber is adjusted to another movement position, make there is pin hole to be lit, enter
Step e；
Step e, obtain the size of hot spot or/and position on imageing sensor；
The size of hot spot or/and position on step f, the imageing sensor obtained according to step e, what judgement was lit is pin hole battle array
Which pin hole on strake；
The pin hole coordinate that step g, basis are lit, judges horizontal position of the containing liquid chamber outgoing directional light relative to incident parallel light
Move；
Step h, the lateral displacement obtained according to step g, with reference to the mobile position of transparent adjustment plate in containing liquid chamber, obtain the folding of solution
Penetrate rate；
Step i, the refractive index according to solution, obtain persticide residue.
The above-mentioned pesticide residue detection method based on point target frequency spectrum detection,
The size of the hot spot described in step f or/and position and be lit is between which pin hole on pinhole array plate
Corresponding relation, is obtained by the method demarcated；
The corresponding relation between pin hole coordinate and lateral displacement described in step g, is obtained by the method demarcated；
The corresponding relation between lateral displacement and refractive index described in step h, is obtained by the method demarcated；
The corresponding relation between refractive index and persticide residue described in step i, is obtained by the method demarcated.
Beneficial effect：
Firstth, present invention finds during the pesticide solution concentration difference, the characteristic of solution refractive index can be changed, has invented one kind and be based on
The pesticide residue detection device and detection method of point target frequency spectrum detection, there is provided a kind of brand-new the Detection Technologies of Pesticide Residues hand
Section.
Secondth, in apparatus of the present invention, using camera bellows, and there is black flannelette in camera bellows inner surface setting, can be to camera bellows
Interior veiling glare is absorbed, and reduces noise jamming.
3rd, in apparatus of the present invention, containing liquid chamber comes down in torrents placement relative to optical axis, is to realize that directional light occurs lateral attitude
Key Design, be that Detecting Pesticide establishes key technology.
4th, in apparatus of the present invention, transparent adjustment plate has two mobile positions, is avoided in specific refractive index situation
Under, got to just between two pin holes of pinhole array plate from the directional light of containing liquid chamber outgoing.
5th, the present invention only needs to light directional light and most successive steps transparent adjustment Board position when operation
Operation, operating process very simple, layman can also operate, and considerably increase the scope of application of device.
6th, after demarcation, the size of hot spot or/and position can directly be obtained the present invention by computer vision technique
, and passing through the size of hot spot or/and position can directly obtain persticide residue, save all intermediate computations links, equally
Layman can just operate, and considerably increase the scope of application of device.
Brief description of the drawings
Fig. 1 is the structural representation of pesticide residue detection device of the present invention based on point target frequency spectrum detection.
In figure：1 containing liquid chamber, 2 pinhole array plates, 3 fourier lenses, 4 imageing sensors.
Specific embodiment
Specific embodiment one
The present embodiment is the embodiment of the pesticide residue detection device based on point target frequency spectrum detection.
The pesticide residue detection device based on point target frequency spectrum detection of the present embodiment, structural representation were as shown in figure 1, should
Detection means is sequentially placed containing liquid chamber 1, pinhole array plate 2, fourier lense 3 in inner surface setting has the camera bellows of black flannelette
With imageing sensor 4；
The containing liquid chamber 1 is placed relative to inclined light shaft, and the outside setting of containing liquid chamber 1 is oriented to be projected as circle perpendicular to optical axial plane
The hole of shape, the directional light parallel with optical axis direction irradiates from outside hole in lateral containing liquid chamber 1, and the inner side of containing liquid chamber 1 is provided with
Mobile transparent adjustment plate, the transparent adjustment plate has two mobile positions, and the inside of containing liquid chamber 1 is equipped with residues of pesticides extract solution；
The pinhole array plate 2 is distributed with multiple arrangements of direction in length and breadth in optical axis direction fixed placement, pinhole array plate 2
Pin hole, the distance between two neighboring pin hole is equal, and not less than hole to the diameter projected perpendicular to optical axial plane；
The fourier lense 3 is perpendicular to optical axis direction fixed placement, and pinhole array plate 2 is located at the first of fourier lense 3
Position of focal plane；
Described image sensor 4 perpendicular to optical axis direction fixed placement, and positioned at the second position of focal plane of fourier lense 3；
The directional light parallel with optical axis direction irradiates from outside hole in lateral containing liquid chamber 1, due to being acted on by residues of pesticides extract solution
Reflect, the light beam from the outgoing of containing liquid chamber 1 is the directional light that there is lateral displacement relative to incident parallel light, outgoing directional light
A pin hole in pinhole array plate 2 is illuminated, the pin hole being illuminated is null tone relation with imageing sensor 4, and then in image
The frequency spectrum of pin hole is obtained on sensor 4.
Specific embodiment two
The present embodiment is the embodiment of the pesticide residue detection method based on point target frequency spectrum detection.
The pesticide residue detection method based on point target frequency spectrum detection of the present embodiment, is based on described in specific embodiment one
Realized on the pesticide residue detection device of point target frequency spectrum detection, the Detecting Pesticide side of point target frequency spectrum detection should be based on
Method, comprises the following steps：
Step a, with transparent solvent extract residues of pesticides, formed with agricultural chemicals as solute, solution of the transparent solvent as solvent；
This step is it should be noted that the application is detected just for the agricultural chemicals with following characteristic：Can be dissolved in transparent molten
Agent, the agricultural chemicals dissolved by transparent solvent；Pesticide concentration change can change the agricultural chemicals of solution refractive index；In this step, specifically
Extracting method, it is next that those skilled in the art can select the determinand of fixed mass that the set time is soaked in fixed volume solvent
Extracted, and the quality of determinand, the volume of solvent, the isoparametric selection of soak time, those skilled in the art are by having
Limit time experiment just can determine, and specific parameter, and be not a unique amount, as long as being demarcated in subsequent steps,
All it is feasible；
Extracted using which type of solvent, Rational choice is carried out according to the species of agricultural chemicals, this is chosen work, for this
It is also what is be easily achieved for art personnel, is not described in detail in this application；
Step b, the solution that step a is obtained is poured into containing liquid chamber 1；
Step c, the irradiation in lateral containing liquid chamber 1 from outside hole with the directional light parallel with optical axis direction；
Step d, observed with imageing sensor 4 and whether there is pin hole to be lit, if：
There is pin hole to be lit, the transparent adjustment plate in containing liquid chamber 1 keeps current mobile position, into step e；
It is pin-free to be lit, the transparent adjustment plate in containing liquid chamber 1 is adjusted to another movement position, make there is pin hole to be lit, enter
Enter step e；
Step e, obtain the size of hot spot or/and position on imageing sensor 4；
The size of hot spot or/and position on step f, the imageing sensor 4 obtained according to step e, what judgement was lit is pin hole
Which pin hole on array board 2；
The pin hole coordinate that step g, basis are lit, judges horizontal position of the outgoing directional light of containing liquid chamber 1 relative to incident parallel light
Move；
Step h, the lateral displacement obtained according to step g, with reference to the mobile position of transparent adjustment plate in containing liquid chamber 1, obtain solution
Refractive index；
Step i, the refractive index according to solution, obtain persticide residue.
Specific embodiment three
The present embodiment is the embodiment of the pesticide residue detection method based on point target frequency spectrum detection.
The pesticide residue detection method based on point target frequency spectrum detection of the present embodiment, on the basis of specific embodiment two
Further limit：
The size of the hot spot described in step f or/and position and be lit is between which pin hole on pinhole array plate 2
Corresponding relation, is obtained by the method demarcated；
The corresponding relation between pin hole coordinate and lateral displacement described in step g, is obtained by the method demarcated；
The corresponding relation between lateral displacement and refractive index described in step h, is obtained by the method demarcated；
The corresponding relation between refractive index and persticide residue described in step i, is obtained by the method demarcated.
It should be noted that the specific demarcation numerical value in specific embodiment two and specific embodiment three, be according to agricultural chemicals
The design parameter of each element could finally confirm in species, the application device, as long as scaling method can be clear and definite, art technology
Personnel are obtained in that demarcation numerical value, because the application provides a kind of technological means, join rather than a set of specific technology
Number, therefore specifically demarcation numerical value is not illustrated in this application；And specific scaling method, the routine of this area is belonged to again
Technological means, the time that dotted timer is obtained when those skilled in the art expect senior middle school and the calibration experiment for putting position, just
How this carries out to be able to know that the application calibration experiment, therefore in this application also without being described in detail.
Claims (1)
1. the pesticide residue detection device of point target frequency spectrum detection is based on, it is characterised in that have black flannelette in inner surface setting
Camera bellows in be sequentially placed containing liquid chamber（1）, pinhole array plate（2）, fourier lense（3）And imageing sensor（4）；
The containing liquid chamber（1）Placed relative to inclined light shaft, containing liquid chamber（1）Outside set it is oriented perpendicular to optical axial plane projection
It is circular hole, the directional light parallel with the optical axis direction lateral containing liquid chamber from outside hole（1）Middle irradiation, containing liquid chamber（1）Inner side set
The transparent adjustment plate that can be moved is equipped with, the transparent adjustment plate has two mobile positions, containing liquid chamber（1）Inside is residual equipped with agricultural chemicals
Stay extract solution；
The pinhole array plate（2）Perpendicular to optical axis direction fixed placement, pinhole array plate（2）On multiple directions in length and breadth are distributed with
The distance between the pin hole of arrangement, two neighboring pin hole are equal, and not less than hole to the diameter projected perpendicular to optical axial plane；
The fourier lense（3）Perpendicular to optical axis direction fixed placement, and pinhole array plate（2）Positioned at fourier lense（3）
The first position of focal plane；
Described image sensor（4）Perpendicular to optical axis direction fixed placement, and positioned at fourier lense（3）The second focal plane position
Put；
The directional light parallel with optical axis direction lateral containing liquid chamber from outside hole（1）Middle irradiation, due to being made by residues of pesticides extract solution
With reflecting, from containing liquid chamber（1）The light beam of outgoing is the directional light that there is lateral displacement relative to incident parallel light, and outgoing is put down
Row light is by pinhole array plate（2）In a pin hole illuminate, the pin hole and imageing sensor being illuminated（4）It is null tone relation, enters
And in imageing sensor（4）On obtain the frequency spectrum of pin hole.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611091982.5A CN106770030B (en) | 2016-12-01 | 2016-12-01 | Pesticide residue detection device based on point target frequency spectrum detection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611091982.5A CN106770030B (en) | 2016-12-01 | 2016-12-01 | Pesticide residue detection device based on point target frequency spectrum detection |
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| CN106770030A true CN106770030A (en) | 2017-05-31 |
| CN106770030B CN106770030B (en) | 2019-07-19 |
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| EP1645864A2 (en) * | 2004-10-07 | 2006-04-12 | Wyatt Technology Corporation | Enhanced sensitivity differential refractometer incorporating a photodetector array |
| CN1936543A (en) * | 2006-10-10 | 2007-03-28 | 中国海洋大学 | Apparatus and method for measuring organic maturity degree of hydrocarbon source rocks |
| CN101995393A (en) * | 2010-09-30 | 2011-03-30 | 哈尔滨工程大学 | Device and method for measuring concentration of liquid based on glass synthetic body and linear array charge-coupled device (CCD) |
| CN103308142A (en) * | 2013-05-28 | 2013-09-18 | 华南师范大学 | Method and device for measuring speed and frequency of ultrasonic traveling wave in liquid |
| CN103645119A (en) * | 2013-12-17 | 2014-03-19 | 哈尔滨工程大学 | Point light source-based measuring device for measuring liquid density of matrix CCD (Charged Coupled Device) |
-
2016
- 2016-12-01 CN CN201611091982.5A patent/CN106770030B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1601244A (en) * | 2003-09-22 | 2005-03-30 | 奥林巴斯株式会社 | Focus detecting unit, and refractive index measuring apparatus and non-contact thermometer using the same |
| EP1645864A2 (en) * | 2004-10-07 | 2006-04-12 | Wyatt Technology Corporation | Enhanced sensitivity differential refractometer incorporating a photodetector array |
| CN1936543A (en) * | 2006-10-10 | 2007-03-28 | 中国海洋大学 | Apparatus and method for measuring organic maturity degree of hydrocarbon source rocks |
| CN101995393A (en) * | 2010-09-30 | 2011-03-30 | 哈尔滨工程大学 | Device and method for measuring concentration of liquid based on glass synthetic body and linear array charge-coupled device (CCD) |
| CN103308142A (en) * | 2013-05-28 | 2013-09-18 | 华南师范大学 | Method and device for measuring speed and frequency of ultrasonic traveling wave in liquid |
| CN103645119A (en) * | 2013-12-17 | 2014-03-19 | 哈尔滨工程大学 | Point light source-based measuring device for measuring liquid density of matrix CCD (Charged Coupled Device) |
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Effective date of registration: 20190621 Address after: 518101 Building 101, Huaxue Testing Building, Xingdong Community, Xin'an Street, Baoan District, Shenzhen City, Guangdong Province Applicant after: CENTRE TESTING INTERNATIONAL GROUP Co.,Ltd. Address before: 150036 Xiangfang District, Harbin, Heilongjiang, 45 East Street five people's livelihood (1 building of Minsheng District) 2 unit 401 room. Applicant before: Wang Qi |
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Effective date of registration: 20221208 Address after: F11-13, Building B, No.1 R&D 1st Road, Songshanhu Hi tech Industrial Development Zone, Dongguan, Guangdong 523000 Patentee after: Dongguan Huace Testing&Certification Co.,Ltd. Patentee after: CENTRE TESTING INTERNATIONAL GROUP Co.,Ltd. Address before: 518101 building 101, No. 1, huatest building, Xingdong community, Xin'an street, Bao'an District, Shenzhen, Guangdong Province Patentee before: CENTRE TESTING INTERNATIONAL GROUP Co.,Ltd. |