CN106768342A - The device and method of unequal interval multiple plane imaging is realized based on palarization multiplexing - Google Patents
The device and method of unequal interval multiple plane imaging is realized based on palarization multiplexing Download PDFInfo
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- CN106768342A CN106768342A CN201611189219.6A CN201611189219A CN106768342A CN 106768342 A CN106768342 A CN 106768342A CN 201611189219 A CN201611189219 A CN 201611189219A CN 106768342 A CN106768342 A CN 106768342A
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- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
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Abstract
The invention discloses a kind of device that unequal interval multiple plane imaging is realized based on palarization multiplexing, the Part I of the device includes light source, the polarizer, beam-expanding collimation device, diaphragm, light-dividing device, the second Amici prism and spatial light modulator, and Part II includes the first speculum, the 3rd Amici prism, the second speculum, the 4th Amici prism, the first lens, analyzer and photoelectric coupled device；Using the device, incident light is divided into two consistent bunch polarised lights of mutually orthogonal and transmission direction first, incide respectively on two parts phase type distortion grating, realize that unequal interval is imaged by the degree of blur for adjusting grating, then the imaging of different diffraction level is chosen by adjusting the polarization angle of analyzer；It is an advantage of the invention that：By adjusting the parameter of two parts grating, multilevel unequal interval imaging is realized, be no longer equidistantly imaging, while institute is optionally observed and studied into image using polarization characteristic, it is adaptable to the uneven object of genesis analysis.
Description
Technical field
The present invention relates to micro-imaging, more particularly to one kind difference is realized on the basis of grating image using palarization multiplexing
The unequal interval multiple plane imaging device that depth object is imaged simultaneously, while relating to the use of the device realizes many planes of unequal interval
The method of imaging.
Background technology
Since initial microscopical birth, the development of light microscope has had the history of more than 400 years.Microscope
The development of technology has largely promoted the process of life science, with the continuous progress of mankind's science and technology, light microscope
Magnifying power already exceed 1000 times.It is stronger and stronger in microscope functions, increasingly hommization while, people want to it
Asking also can more and more higher.Essentially consist in the microscopical depth of field and resolution ratio has contradiction：When needing, collection three-dimensional body multiple is deep
During strength information at degree, because the depth of field of micro imaging system is limited, especially in the microcobjective using large-numerical aperture
When, even small defocus can also cause that microscope cannot be clearly imaged.We have to move in the vertical and treat
Test sample product and microcobjective, in this process error be not avoided that, and because object distance changes, the horizontal magnification of sample
Rate also can be different.Therefore, on-mechanical movement realizes many planes while becoming a heat of micro-imaging technique at this stage
Point problem.
Blanchard and Greenaway proposed amplitude type to distort grating and lens combination in 1999, successfully by three
The object of individual different depth is imaged simultaneously in grating different diffraction level, and grating diffration efficiency ratio is distorted yet with amplitude type
Relatively low, generally only 0 grade and ± 1 grade is used effectively, so Blanchard and Greenaway proposes that phase type can be used
Grating to improve diffraction efficiency, (order of diffraction quantity is usual<10).Phase type distortion grating technology must in micro-imaging field
To being increasingly widely applied, such as imaging and tracking, fluid velocity measurement of many plane 3D particles etc., by observing object
The change being imaged in nine planes carrys out position and the speed of judgment object.It will be further appreciated that, the technology can reach to be received
The axial resolution of meter level, so having very big application space in micro- field.Relative to scanning imagery, these are traditional more
For planar imaging method, the technology not only avoids the error that Mechanical Moving in imaging process is brought, and eliminates and sweep
The time of object and reconstruction image is retouched, multilevel real time imagery is realized.Zhou Changhe in 2013 etc. is introduced in grating is distorted
Darman raster, also allows that more diffraction times are utilized (reached 7*7).Zhou Changhe in 2016 etc. is improved and reached again
Graceful grating, further increases distortion grating diffration efficiency.Although the introducing of Darman raster is in the quantity of effective order of diffraction
It is greatly improved, but, with the increase of the quantity of the order of diffraction, due to the limited area of CCD target surfaces, can get and regard
Field just relative reduction, and equidistant object aspect can only be carried out to be imaged simultaneously.
The content of the invention
Goal of the invention：The first object of the present invention is that the defect existed for prior art proposes a kind of multiple based on polarization
The device of unequal interval multiple plane imaging is realized with technology；The second object of the present invention is to provide between utilizing the device to realize not waiting
Every the method for multiple plane imaging.
Technical scheme：The present invention adopts the following technical scheme that realization：It is a kind of to realize that unequal interval is put down more based on palarization multiplexing
The device of face imaging, including the Part I modulated for light splitting and the Part II for focal imaging, Part I include
Light source, the polarizer, beam-expanding collimation device, diaphragm, light-dividing device, the second Amici prism and it is loaded with the space of grating A and grating B
Optical modulator, each element is along optical path direction successively straight line；Wherein, light-dividing device includes polarization splitting prism and the first light splitting
Prism, object light light beam r is divided into two linearly polarized lights that beam polarization state is mutually orthogonal and transmission direction is consistent by light-dividing device, and
The half-wave plate for adjusting incident light polarization angle is provided between first Amici prism and the second Amici prism；Part II includes
Collinear 3rd Amici prism, the 4th Amici prism are located at half-wave plate, grating B, grating diffration light is focused on the
First speculum and the second speculum of four Amici prisms, for the photoelectric coupled device that is imaged and positioned at the 4th Amici prism and
The first lens and analyzer between photoelectric coupled device.
Further, the target surface of spatial light modulator is divided into two, and grating A and grating B loads on two of target surface respectively
Part, and grating A and grating B are phase twist type grating.
Preferably, beam-expanding collimation device includes the microcobjective for expanding and the second lens for collimating.
The method that unequal interval multiple plane imaging is realized based on palarization multiplexing, is comprised the following steps：
A () opens light source, required grating is loaded into spatial light modulator, adjusts the parameter of grating A and grating B, obtains
To clearly imaging effect；
B () light source passes sequentially through the variant aspect of sample M, the object light light beam of generation through the linearly polarized light that the polarizer is modulated
The two line polarization light that beam polarization state is mutually orthogonal and the direction of propagation is consistent are divided into by light-dividing device, then by the second Amici prism point
It is not irradiated in spatial light modulator；
C () light beam r2 is irradiated to grating A, the diffraction light of generation is reflexed on the 4th Amici prism through the first speculum；
D () light beam r1 is irradiated to grating B, the diffraction light of generation reflexes to the 4th through the 3rd Amici prism and the second speculum
On Amici prism；
E () diffraction light a and diffraction light b converge through the 4th Amici prism, then via the first lens focus to photoelectrical coupler
Part receives imaging；Wherein, the position of the order of diffraction is finely tuned by adjusting the first speculum and the second speculum, makes grating A and grating B
0 grade for producing overlaps, and secondly, the specific order of diffraction is chosen by the angle for adjusting analyzer, and the difference of observation sample M is deep
Degree aspect.
Beneficial effect：Compared with prior art, the present invention distorts grating by multilayer graph based on palarization multiplexing using phase type
As being imaged in the same plane, and the above mutually orthogonal polarization state of the order of diffraction coupling of X and Y-direction is caused, by adjusting two
The parameter of light splitting grid, realizes multilevel unequal interval imaging, is no longer equidistantly imaging, while can be right using polarization characteristic
Formed image carries out selective observation and research, it is adaptable to the uneven object of genesis analysis.
Brief description of the drawings
Fig. 1 is apparatus structure schematic diagram of the invention；
Fig. 2 is the imaging schematic diagram of the embodiment of the present invention；
Fig. 3 is polarization state orthogonality principle figure of the invention.
Specific embodiment
Technical scheme is described further below in conjunction with the accompanying drawings.
As shown in figure 1, the device of unequal interval multiple plane imaging is realized based on palarization multiplexing, including modulated for light splitting
Part I and diffraction light is pooled the Part II of picture, Part I includes light source 1, the polarizer 2, beam-expanding collimation device
3rd, diaphragm 4, be made up of the Amici prism 502 of polarization splitting prism 501 and first light-dividing device 5, the second Amici prism 6, be located at
Half-wave plate 7 between first Amici prism 502 and the second Amici prism 6 and it is loaded with phase twist type grating A and phase twist
The spatial light modulator 8 of type grating B, it is preferred that the microcobjective 301 of beam-expanding collimation device 3 and the second lens 302 are constituted, wherein,
Microcobjective 301 is used to expand, and the second lens 302 are used to collimate；
Light source 1, the polarizer 2, beam-expanding collimation device 3, diaphragm 4, the Amici prism 6 of light-dividing device 5 and second are along optical path direction
Straight line arrangement, has the requirement of polarization angle due to spatial light modulator 8, it is desirable to the polarization direction of incident light to incident light successively
Long side direction with the display board of spatial light modulator 8 is consistent, in polarization direction and the display board long side direction of spatial light modulator 8 not
Consistent light beam r2 to be incided and add half-wave plate 7 at the position before spatial light modulator 8 so that the incident light meets spatial light tune
The use requirement of device processed 8, can obtain the mutually orthogonal emergent light of polarization state again.
Part II act as, the diffraction light focal imaging that two light beams in Part I are produced, specifically include with
Half-wave plate 7, grating B are located at collinear 3rd Amici prism 10, the 4th Amici prism 12, focus on grating diffration light
First speculum 9 and the second speculum 11 of the 4th Amici prism 12, for be imaged photoelectric coupled device 15 and positioned at the 4th
The first lens 13 and analyzer 14 between Amici prism 12 and photoelectric coupled device 15.
The method for realizing unequal interval multiple plane imaging using the present apparatus is：
A () simulates required raster pattern on computers first, open light source 1, and grating is loaded into spatial light modulator 8
Target surface on, wherein, the target surface of spatial light modulator 8 is divided into two, and two different phase twist type grating A are loaded with respectively
With phase twist type grating B；Wherein, the direction of two parts grating differs 90 degree, i.e., must 90 degree of one of grating rotating
To second grating, more gratings are loaded in spatial light modulator 8 in theory can obtain more information, but be subject to
The limitation of the target surface size of spatial light modulator 8 and photoelectric coupled device 15, so the quantity of loading grating is restricted, preferably
, grating is divided into two parts；The parameter of adjustment phase place distortion type grating A and phase twist type grating B, is clearly imaged
Effect；
B one-wavelength laser that () light source 1 sends is sequentially passed through in beam-expanding collimation device 3 through the line polarization light that the polarizer 2 is obtained
The lens 302 of microcobjective 301 and second, and pass through sample M through diaphragm 4, form object light light beam r, object light light beam r first passes around
Polarization splitting prism 501 in light-dividing device 5 is divided into the mutually orthogonal light beam r1 and light beam r2 of polarization state, and light beam r1 and the original
The direction of propagation of light light beam r is consistent, and the direction of propagation and the light beam r1 of light beam r2 are in 90 °, and the first Amici prism 502 is again by light beam r2
The direction of propagation change 90 °, make light beam r2 and light beam r1 as the linearly polarized light that polarization state is mutually orthogonal and transmission direction is consistent,
On the grating A and grating B being radiated at respectively in spatial light modulator 8 through the Amici prism 6 of half-wave plate 7 and second again；
C diffraction light a that () grating A is produced is reflexed on the 4th Amici prism 12 through the first speculum 9, what grating B was produced
Diffraction light b is reflexed on the 4th Amici prism 12 via the 3rd Amici prism 10 and the second speculum 11, reflexes to the 4th light splitting
Two-beam on prism 12 is focused on by the first lens 13 and imaging is received on photoelectric coupled device 15, by adjusting the first speculum
9 and second speculum 11 finely tune the position of the order of diffraction of two-beam generation so that produce 0 grade of grating A and grating B being capable of phase
Mutually overlap, make full use of the effective coverage of the target surface of photoelectric coupled device 15 to receive image-forming information；
As shown in Fig. 2 sample M is with eight objects of different aspects, object light light beam r forms inclined after light-dividing device 5
Polarization state different light beam r1 and light beam r2, two-beam are the linearly polarized light that polarization state is mutually orthogonal and transmission direction is consistent, are passed through
Different distance is diffracted into the surface of spatial light modulator 8, wherein, light beam r2 is irradiated to grating A, and light beam r1 is irradiated to grating B, if spreading out
It is respectively A, B, C, D, E, F, G, H to penetrate distance, and A ＞ B ＞ C ＞ D ＞ E ＞ F ＞ G ＞ H；Again through each element of device Part II
Converge and image on photoelectric coupled device 15, big letter is expressed as clearly, small letter is expressed as fuzzy.
As shown in figure 3, eight different object aspects of 1,2,3,4,5,6,7,8 representative sample M, each object aspect to light
The distance of grid is different.Marked as odd number object aspect through grating A be imaged, and institute into polarization direction be level, such as in figure
Shown in horizontal double-headed；Object aspect marked as even numbers is imaged through grating B, and imaging polarization direction is vertical, such as in figure
Shown in vertical double-head arrow；Wherein 1 and 2 distance for d1,2 and 3 distance for d2, d1 and d2 can be by adjusting the parameter of grating
To change.Compared with the technology for only realizing multiple plane imaging by phase twist type grating, the characteristic first with grating of the invention
So that eight object aspects are imaged on the same plane, by adjust grating A and the degree of blur of grating B realize unequal interval into
Picture, object than it is larger when, each order of diffraction imaging respectively correspond to different depth object, now only need to adjust photoelectricity
Analyzer before coupled apparatus 15 can be obtained by the information of different depth aspect object, it is adaptable to moving object, active object
The imaging of tissue.
Claims (4)
1. a kind of device that unequal interval multiple plane imaging is realized based on palarization multiplexing, it is characterised in that：Described device includes using
In the Part I and the Part II for focal imaging of light splitting modulation, Part I includes light source (1), the polarizer (2), expands
Beam collimator apparatus (3), diaphragm (4), light-dividing device (5), the second Amici prism (6) and it is loaded with the spatial light of grating A and grating B
Modulator (8), each element is along optical path direction successively straight line；Wherein, light-dividing device (5) includes polarization splitting prism (501)
With the first Amici prism (502), object light light beam r is divided into that two beam polarization states are mutually orthogonal and transmission direction by light-dividing device (5)
Consistent linearly polarized light, and be provided between the first Amici prism (502) and the second Amici prism (6) inclined for adjusting incident light
The half-wave plate (7) of angle of shaking；Part II includes being located at collinear 3rd Amici prism with half-wave plate (7), grating B
(10), the 4th Amici prism (12), grating diffration light is focused on first speculum (9) and of the 4th Amici prism (12)
Two-mirror (11), for be imaged photoelectric coupled device (15) and positioned at the 4th Amici prism (12) and photoelectric coupled device
(15) the first lens (13) and analyzer (14) between.
2. the device that unequal interval multiple plane imaging is realized based on palarization multiplexing according to claim 1, the spatial light
The target surface of modulator (8) is divided into two, and grating A and grating B loads on two parts of target surface respectively, and grating A and grating B are equal
For phase type distorts grating.
3. the device that unequal interval multiple plane imaging is realized based on palarization multiplexing according to claim 1, it is characterised in that：
Second lens (302) of the beam-expanding collimation device (3) including the microcobjective (301) for expanding and for collimating.
4. the method that the device described in a kind of utilization claim 1 realizes unequal interval multiple plane imaging, it is characterised in that including
Following steps：
A () opens light source (1), required grating is loaded into spatial light modulator (8), adjusts the parameter of grating A and grating B,
Obtain clearly imaging effect；
B () light source (1) passes sequentially through the variant aspect of sample M, the object light light of generation through the linearly polarized light that the polarizer (2) is modulated
Beam r is divided into two the light beam r1 and light beam r2 that beam polarization state is mutually orthogonal and the direction of propagation is consistent by light-dividing device (5), then by
Two Amici prisms (6) are respectively radiated in spatial light modulator (8)；
C () light beam r2 is irradiated to grating A, the diffraction light a of generation reflexes to the 4th Amici prism (12) through the first speculum (9)
On；
D () light beam r1 is irradiated to grating B, the diffraction light b of generation reflects through the 3rd Amici prism (10) and the second speculum (11)
Onto the 4th Amici prism (12)；
E () diffraction light a and diffraction light b converges through the 4th Amici prism (12), then focus on light thermocouple via the first lens (13)
Clutch part (15) receives imaging；Wherein, the position of the order of diffraction is finely tuned by adjusting the first speculum (9) and the second speculum (11)
Put, produce grating A and grating B 0 grade overlaps, secondly, chosen by the angle for adjusting analyzer (14) and specifically spread out
Penetrate level, the different depth aspect of observation sample M.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108663735A (en) * | 2018-04-17 | 2018-10-16 | 中国科学院上海光学精密机械研究所 | The real-time 3D of achromatism based on distortion Darman raster is imaged microscope equipment |
| CN116540212A (en) * | 2023-07-06 | 2023-08-04 | 中国空气动力研究与发展中心超高速空气动力研究所 | Three-dimensional imaging device and method |
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| US20110007307A1 (en) * | 2009-07-09 | 2011-01-13 | Joseph Daniel Vance | Two-Photon-Absorption Optical Filter |
| CN202195882U (en) * | 2011-08-12 | 2012-04-18 | 谭成忠 | Fourier spectrometer without movable mechanical part |
| US8947663B2 (en) * | 2013-03-15 | 2015-02-03 | The Trustees Of Princeton University | Dual-modulation faraday rotation spectroscopy |
| CN104880253A (en) * | 2014-02-18 | 2015-09-02 | 哈尔滨工业大学 | High-spatial-resolution snapshot-type imaging method based on polarizing optical splitters |
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2016
- 2016-12-21 CN CN201611189219.6A patent/CN106768342B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110007307A1 (en) * | 2009-07-09 | 2011-01-13 | Joseph Daniel Vance | Two-Photon-Absorption Optical Filter |
| CN202195882U (en) * | 2011-08-12 | 2012-04-18 | 谭成忠 | Fourier spectrometer without movable mechanical part |
| US8947663B2 (en) * | 2013-03-15 | 2015-02-03 | The Trustees Of Princeton University | Dual-modulation faraday rotation spectroscopy |
| CN104880253A (en) * | 2014-02-18 | 2015-09-02 | 哈尔滨工业大学 | High-spatial-resolution snapshot-type imaging method based on polarizing optical splitters |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108663735A (en) * | 2018-04-17 | 2018-10-16 | 中国科学院上海光学精密机械研究所 | The real-time 3D of achromatism based on distortion Darman raster is imaged microscope equipment |
| CN116540212A (en) * | 2023-07-06 | 2023-08-04 | 中国空气动力研究与发展中心超高速空气动力研究所 | Three-dimensional imaging device and method |
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