CN106768342B - The device and method of unequal interval multiple plane imaging is realized based on palarization multiplexing - Google Patents
The device and method of unequal interval multiple plane imaging is realized based on palarization multiplexing Download PDFInfo
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- CN106768342B CN106768342B CN201611189219.6A CN201611189219A CN106768342B CN 106768342 B CN106768342 B CN 106768342B CN 201611189219 A CN201611189219 A CN 201611189219A CN 106768342 B CN106768342 B CN 106768342B
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- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
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Abstract
The invention discloses a kind of devices for realizing unequal interval multiple plane imaging based on palarization multiplexing, the first part of the device includes light source, the polarizer, beam-expanding collimation device, diaphragm, light-dividing device, the second Amici prism and spatial light modulator, and second part includes the first speculum, third Amici prism, the second speculum, the 4th Amici prism, the first lens, analyzer and photoelectric coupled device；Utilize the device, incident light is divided into two consistent bunch polarised lights of mutually orthogonal and transmission direction first, it is incident on respectively on two parts phase type distortion grating, unequal interval imaging is realized by adjusting the degree of blur of grating, then the imaging of the different orders of diffraction is chosen by adjusting the polarization angle of analyzer；It is an advantage of the invention that：By adjusting the parameter of two parts grating, multilevel unequal interval imaging is realized, be no longer equidistantly to be imaged, while at image selectively observe and study to institute using polarization characteristic, be suitable for the non-uniform object of genesis analysis.
Description
Technical field
The present invention relates to micro-imagings, more particularly to one kind realizing difference using palarization multiplexing on the basis of grating image
The unequal interval multiple plane imaging device that depth object is imaged simultaneously, while relating to the use of the device and realizing the more planes of unequal interval
The method of imaging.
Background technology
Since initial microscopical birth, the development of light microscope has had more than 400 years history.Microscope
The development of technology has largely pushed the process of life science, with being constantly progressive for mankind's science and technology, light microscope
Magnifying power already exceed 1000 times.It is stronger and stronger in microscope functions, increasingly hommization while, people want it
Asking also can be higher and higher.Essentially consisting in the microscopical depth of field and resolution ratio, there are contradictions：When need acquire the multiple depths of three-dimension object
When strength information at degree, since the depth of field of micro imaging system is limited, especially in the microcobjective using large-numerical aperture
When, even small defocus can also make microscope is unable to get clearly to be imaged.We, which have to move in the longitudinal direction, waits for
Sample and microcobjective, in this process error be not avoided that, and since object distance changes, the horizontal magnification of sample
Rate also can be different.Therefore, on-mechanical movement realizes that more planes become a heat of micro-imaging technique at this stage simultaneously
Point problem.
Blanchard and Greenaway proposed amplitude type to distort grating and lens combination in 1999, successfully by three
The object of a different depth is imaged simultaneously in grating difference diffraction time, however since amplitude type distorts grating diffration efficiency ratio
Relatively low, usually only 0 grade and ± 1 grade is used effectively, so Blanchard and Greenaway propositions can use phase type
Grating to improve diffraction efficiency, (order of diffraction quantity is usual<10).Phase type distorts grating technology must in micro-imaging field
To being more and more widely used, for example imaging and tracking, the fluid velocity of more plane 3D particles measure etc., pass through and observe object
The variation being imaged in nine planes carrys out the position and speed of judgment object.It is received it will be further appreciated that the technology can reach
The axial resolution of meter level, so having prodigious application space in micro- field.Relative to scanning imagery, these are traditional more
For planar imaging method, which not only avoids error caused by Mechanical Moving in imaging process, and eliminates and sweep
The time for retouching object and reconstruction image realizes multilevel real time imagery.Zhou Changhe in 2013 etc. is introduced in distorting grating
Darman raster, but also more diffraction times can be utilized and (reach 7*7).Zhou Changhe in 2016 etc. is improved again to be reached
Graceful grating further improves distortion grating diffration efficiency.Although the introducing of Darman raster is in the quantity of effective order of diffraction
It is greatly improved, still, as the increase of the quantity of the order of diffraction can be got and be regarded due to the limited area of CCD target surfaces
Field is just opposite to be reduced, and equidistant object level can only be carried out while is imaged.
Invention content
Goal of the invention：The first object of the present invention is to propose in view of the defects existing in the prior art a kind of multiple based on polarization
The device of unequal interval multiple plane imaging is realized with technology；The second object of the present invention is to provide using between device realization grade
Every the method for multiple plane imaging.
Technical solution：The present invention adopts the following technical scheme that realization：One kind realizing that unequal interval is mostly flat based on palarization multiplexing
Face imaging device, include for be divided modulate first part and for the second part of focal imaging, first part includes
Light source, the polarizer, beam-expanding collimation device, diaphragm, light-dividing device, the second Amici prism and the space for being loaded with grating A and grating B
Optical modulator, each element are arranged along optical path direction successively straight line；Wherein, light-dividing device includes polarization splitting prism and the first light splitting
Prism, object light light beam r are divided into two linearly polarized lights that beam polarization state is mutually orthogonal and transmission direction is consistent by light-dividing device, and
The half-wave plate for adjusting incident light polarization angle is equipped between spatial light modulator and the second Amici prism；Second part includes
It is located at collinear third Amici prism, the 4th Amici prism with half-wave plate, grating B, grating diffration light is focused on
The first speculum and the second speculum of four Amici prisms, for the photoelectric coupled device of imaging and positioned at the 4th Amici prism and
The first lens between photoelectric coupled device and analyzer.
Further, the target surface of spatial light modulator is divided into two, and grating A and grating B load on two of target surface respectively
Part, and grating A and grating B are phase twist type grating.
Preferably, beam-expanding collimation device includes the microcobjective for expanding and the second lens for collimation.
Based on the method that palarization multiplexing realizes unequal interval multiple plane imaging, include the following steps：
(a) light source is opened, required grating is loaded into spatial light modulator, the parameter of grating A and grating B is adjusted, obtains
To clearly imaging effect；
(b) linearly polarized light that light source is modulated through the polarizer passes sequentially through the variant level of sample M, the object light light beam of generation
The two line polarization light that beam polarization state is mutually orthogonal and the direction of propagation is consistent are divided by light-dividing device, using the second Amici prism point
It is not irradiated in spatial light modulator；
(c) light beam r2 is irradiated to grating A, and the diffraction light of generation is reflected into through the first speculum on the 4th Amici prism；
(d) light beam r1 is irradiated to grating B, and the diffraction light of generation is reflected into the 4th through third Amici prism and the second speculum
On Amici prism；
(e) diffraction light a and diffraction light b converge through the 4th Amici prism, then via the first lens focus to photoelectrical coupler
Part receives imaging；Wherein, the position that the order of diffraction is finely tuned by adjusting the first speculum and the second speculum, makes grating A and grating B
0 grade generated overlaps, and secondly, chooses the specific order of diffraction by adjusting the angle of analyzer, the difference of observation sample M is deep
Degree level.
Advantageous effect：Compared with prior art, the present invention is based on palarization multiplexings distorts grating by multilayer graph using phase type
As being imaged in the same plane, and make the upper mutually orthogonal polarization state of the order of diffraction of X and Y-direction coupling, by adjusting two
It is divided the parameter of grid, realizes multilevel unequal interval imaging, is no longer equidistantly to be imaged, while utilizing polarization characteristic can be right
Formed image carries out the observation and research of selectivity, is suitable for the non-uniform object of genesis analysis.
Description of the drawings
Fig. 1 is the device of the invention structural schematic diagram；
Fig. 2 is the imaging schematic diagram of the embodiment of the present invention；
Fig. 3 is the polarization state orthogonality principle figure of the present invention.
Specific implementation mode
Technical scheme of the present invention is described further below in conjunction with the accompanying drawings.
Include for being divided modulation as shown in Figure 1, realizing the device of unequal interval multiple plane imaging based on palarization multiplexing
First part and the second part for being imaged diffraction light convergence, first part includes light source 1, the polarizer 2, beam-expanding collimation device
3, diaphragm 4, be made of polarization splitting prism 501 and the first Amici prism 502 light-dividing device 5, the second Amici prism 6, be set to
Half-wave plate 7 between spatial light modulator 8 and the second Amici prism 6 and it is loaded with phase twist type grating A and phase twist type
The spatial light modulator 8 of grating B, it is preferred that 3 microcobjective 301 of beam-expanding collimation device and the second lens 302 composition, wherein aobvious
Speck mirror 301 is for expanding, and the second lens 302 are for collimating；
Light source 1, the polarizer 2, beam-expanding collimation device 3, diaphragm 4, light-dividing device 5 and the second Amici prism 6 are along optical path direction
Straight line is arranged successively, since spatial light modulator 8 has incident light the requirement of polarization angle, it is desirable that the polarization direction of incident light
It is consistent with the long side direction of 8 display board of spatial light modulator, polarization direction and 8 display board long side direction of spatial light modulator not
Consistent light beam r1 is incident at the position before spatial light modulator 8 and adds half-wave plate 7 so that the incident light meets spatial light tune
The requirement of device 8 processed, and the mutually orthogonal emergent light of polarization state can be obtained.
Second part is act as, by first part two light beams generate diffraction light focal imaging, specifically include with
Half-wave plate 7, grating B are located at collinear third Amici prism 10, the 4th Amici prism 12, focus on grating diffration light
The first speculum 9 and the second speculum 11 of 4th Amici prism 12, for imaging photoelectric coupled device 15 and be located at the 4th
The first lens 13 between Amici prism 12 and photoelectric coupled device 15 and analyzer 14.
It is using the method that the present apparatus realizes unequal interval multiple plane imaging：
(a) it simulates required raster pattern on computers first, opens light source 1, grating is loaded into spatial light modulator 8
Target surface on, wherein the target surface of spatial light modulator 8 is divided into two, and is loaded with two different phase twist type grating A respectively
With phase twist type grating B；Wherein, the direction of two parts grating differs 90 degree, i.e., must 90 degree of one of grating rotating
To second grating, more gratings are theoretically loaded in spatial light modulator 8 can obtain more information, but by
The limitation of the target surface size of spatial light modulator 8 and photoelectric coupled device 15, so the quantity of load grating is restricted, preferably
, grating is divided into two parts；The parameter of adjustment phase place distortion type grating A and phase twist type grating B, are clearly imaged
Effect；
(b) the line polarization light that the one-wavelength laser that light source 1 is sent out is obtained through the polarizer 2 passes through in beam-expanding collimation device 3 successively
Microcobjective 301 and the second lens 302, and penetrate sample M through diaphragm 4, form object light light beam r, object light light beam r first passes around
Polarization splitting prism 501 in light-dividing device 5 is divided into polarization state mutually orthogonal light beam r1 and light beam r2, and light beam r1 and the original
The direction of propagation of light light beam r is consistent, and the direction of propagation and the light beam r1 of light beam r2 are in 90 °, and the first Amici prism 502 is again by light beam r2
The direction of propagation change 90 °, make light beam r2 and light beam r1 as the linearly polarized light that polarization state is mutually orthogonal and transmission direction is consistent,
It is radiated at respectively on the grating A and grating B in spatial light modulator 8 through half-wave plate 7 and the second Amici prism 6 again；
(c) the diffraction light a that grating A is generated is reflected into through the first speculum 9 on the 4th Amici prism 12, what grating B was generated
Diffraction light b is reflected into via third Amici prism 10 and the second speculum 11 on the 4th Amici prism 12, is reflected into the 4th light splitting
Two-beam on prism 12, which is focused on to receive on photoelectric coupled device 15 by the first lens 13, to be imaged, by adjusting the first speculum
9 and second speculum 11 finely tune the position of the order of diffraction of two-beam generation so that generate 0 grade of grating A and grating B being capable of phase
It mutually overlaps, the effective coverage of the target surface of photoelectric coupled device 15 is made full use of to receive image-forming information；
As shown in Fig. 2, sample M is the object with eight different levels, object light light beam r is formed inclined after light-dividing device 5
Polarization state different light beam r1 and light beam r2, two-beam are the linearly polarized light that polarization state is mutually orthogonal and transmission direction is consistent, are passed through
Different distance is diffracted into 8 surface of spatial light modulator, wherein light beam r2 is irradiated to grating A, and light beam r1 is irradiated to grating B, if spreading out
It is A, B, C, D, E, F, G, H to penetrate distance respectively, and A ＞ B ＞ C ＞ D ＞ E ＞ F ＞ G ＞ H；Each element through device second part again
Converge and image on photoelectric coupled device 15, big letter is expressed as clear, and small letter is expressed as fuzzy.
As shown in figure 3, eight different object levels of 1,2,3,4,5,6,7,8 representative sample M, each object level to light
The distance of grid is different.Object level marked as odd number is imaged through grating A, and institute is level at polarization direction, in figure
Shown in horizontal double-headed；Object level marked as even numbers is imaged through grating B, and imaging polarization direction is vertical, in figure
Shown in vertical double-head arrow；The distance that wherein 1 and 2 distance is d1,2 and 3 is that d2, d1 and d2 can be by adjusting the parameters of grating
To change.Compared with the technology for only realizing multiple plane imaging by phase twist type grating, the invention firstly uses the characteristics of grating
So that eight object levels same plane be imaged, by adjusting the degree of blur of grating A and grating B realize unequal interval at
Picture, when object is bigger, the imaging of each order of diffraction corresponds to the object of different depth respectively, only needs to adjust photoelectricity at this time
Analyzer before coupled apparatus 15 can be obtained by the information of different depth level object, be suitable for moving object, active object
The imaging of tissue.
Claims (4)
1. a kind of device for realizing unequal interval multiple plane imaging based on palarization multiplexing, it is characterised in that：Described device includes using
In the first part of light splitting modulation and for the second part of focal imaging, first part includes light source (1), the polarizer (2), expands
Beam collimator apparatus (3), diaphragm (4), light-dividing device (5), the second Amici prism (6) and the spatial light for being loaded with grating A and grating B
Modulator (8), each element are arranged along optical path direction successively straight line；Wherein, light-dividing device (5) includes polarization splitting prism (501)
With the first Amici prism (502), object light light beam r is divided into that two beam polarization states are mutually orthogonal and transmission direction by light-dividing device (5)
Consistent linearly polarized light, and be equipped between spatial light modulator (8) and the second Amici prism (6) for adjusting incident light polarization
The half-wave plate (7) of angle；Second part include with half-wave plate (7), grating B be located at collinear third Amici prism (10),
4th Amici prism (12), the first speculum (9) that grating diffration light is focused on to the 4th Amici prism (12) and second are anti-
Penetrate mirror (11), for imaging photoelectric coupled device (15) and positioned at the 4th Amici prism (12) and photoelectric coupled device (15) it
Between the first lens (13) and analyzer (14).
2. the device according to claim 1 for realizing unequal interval multiple plane imaging based on palarization multiplexing, the spatial light
The target surface of modulator (8) is divided into two, and grating A and grating B load on two parts of target surface respectively, and grating A and grating B are equal
Grating is distorted for phase type.
3. the device according to claim 1 for realizing unequal interval multiple plane imaging based on palarization multiplexing, it is characterised in that：
The beam-expanding collimation device (3) includes the microcobjective (301) for expanding and the second lens (302) for collimation.
4. a kind of method for realizing unequal interval multiple plane imaging using device described in claim 1, which is characterized in that including
Following steps：
(a) light source (1) is opened, required grating is loaded into spatial light modulator (8), adjusts the parameter of grating A and grating B,
Obtain clearly imaging effect；
(b) linearly polarized light that light source (1) is modulated through the polarizer (2) passes sequentially through the variant level of sample M, the object light light of generation
Beam r is divided into two the light beam r1 and light beam r2 that beam polarization state is mutually orthogonal and the direction of propagation is consistent by light-dividing device (5), using
Two Amici prisms (6) are respectively radiated in spatial light modulator (8)；
(c) light beam r2 is irradiated to grating A, and the diffraction light a of generation is reflected into the 4th Amici prism (12) through the first speculum (9)
On；
(d) light beam r1 is irradiated to grating B, and the diffraction light b of generation is reflected through third Amici prism (10) and the second speculum (11)
Onto the 4th Amici prism (12)；
(e) diffraction light a and diffraction light b converges through the 4th Amici prism (12), then focuses on photoelectricity coupling via the first lens (13)
Clutch part (15) receives imaging；Wherein, the position of the order of diffraction is finely tuned by adjusting the first speculum (9) and the second speculum (11)
It sets, makes 0 grade that grating A and grating B are generated to overlap, secondly, specifically spread out by adjusting the angle selection of analyzer (14)
Penetrate grade, the different depth level of observation sample M.
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| CN116540212A (en) * | 2023-07-06 | 2023-08-04 | 中国空气动力研究与发展中心超高速空气动力研究所 | Three-dimensional imaging device and method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN202195882U (en) * | 2011-08-12 | 2012-04-18 | 谭成忠 | Fourier spectrometer without movable mechanical part |
| US8947663B2 (en) * | 2013-03-15 | 2015-02-03 | The Trustees Of Princeton University | Dual-modulation faraday rotation spectroscopy |
| CN104880253A (en) * | 2014-02-18 | 2015-09-02 | 哈尔滨工业大学 | High-spatial-resolution snapshot-type imaging method based on polarizing optical splitters |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202195882U (en) * | 2011-08-12 | 2012-04-18 | 谭成忠 | Fourier spectrometer without movable mechanical part |
| US8947663B2 (en) * | 2013-03-15 | 2015-02-03 | The Trustees Of Princeton University | Dual-modulation faraday rotation spectroscopy |
| CN104880253A (en) * | 2014-02-18 | 2015-09-02 | 哈尔滨工业大学 | High-spatial-resolution snapshot-type imaging method based on polarizing optical splitters |
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