CN106754327B - Oscillating device for drug sensitivity and control method thereof - Google Patents
Oscillating device for drug sensitivity and control method thereof Download PDFInfo
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- CN106754327B CN106754327B CN201710038381.6A CN201710038381A CN106754327B CN 106754327 B CN106754327 B CN 106754327B CN 201710038381 A CN201710038381 A CN 201710038381A CN 106754327 B CN106754327 B CN 106754327B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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Abstract
The invention relates to an oscillating device for drug sensitivity and a control method thereof. The motor and the displacement platform are arranged at the center of the bottom plate and positioned on the same straight line. According to the oscillating device, a motor shaft is fixedly connected with a coupling through a screw, and the other end of the coupling is fixedly connected to a rotating shaft of a displacement platform through the screw. One end, close to the motor, of the displacement platform rotating shaft is fixedly connected with the kit tray through a conversion piece by a screw. A plurality of reagent box placing areas are arranged on the reagent box tray. One end of the upright post is connected to four corners of the bottom plate through threads. The second layer can be added at the other end of the upright post according to the number of the required reagent boxes, and the structure on the second layer is the same as that of the first layer. In the whole oscillating device, a multi-layer structure is provided, each of which is controlled individually. The motor rotates positively and negatively to form an oscillating effect. The probability of the bacteria fully contacting with the medicine and oxygen in the medicine sensitization process is increased, and the time of the medicine sensitization process is shortened.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to an oscillating device for drug sensitivity and a control method thereof.
Background
As microbial resistance becomes more severe, infectious diseases require accurate identification of pathogenic bacteria species and testing of their in vitro antimicrobial drug sensitivity (AST), providing a clinical medication reference. Currently, clinic relies mainly on manual methods or commercial analysis systems to conduct the tests. The traditional manual method and commercial analysis system adopts a final value method, namely bacterial colonies are diluted by a certain multiple and added into antibiotic kits coated with different types and concentrations, the antibiotic kits are placed into an incubator for culturing for 8-12 hours, and the turbidity of liquid in small holes of the kit is observed with naked eyes or the drug resistance of bacteria is judged by an instrument interpretation mode. The whole culture process is always in a static state, which is not beneficial to the full contact of bacteria and medicines and the supply of oxygen in the culture process. The whole process needs to take a long time, and the requirement of clinic on rapid detection of the project cannot be met.
Disclosure of Invention
(one) solving the technical problems
Aiming at the defects of the prior art, the invention provides the oscillating device for drug sensitization and the control method thereof, so that the probability of full contact between bacteria, drugs and oxygen in the drug sensitization process is increased, and the time of the drug sensitization process is shortened.
(II) technical scheme
In order to achieve the above purpose, the invention is realized by the following technical scheme:
an oscillation device for drug sensitivity comprises a bottom plate, a motor, a displacement platform and an upright post; the method is characterized in that: the device also comprises a photoelectric sensor, an optical detection device, a control circuit and a signal processing control unit, wherein the optical detection device comprises a white light LED array, an LED driving circuit, an optical collimation component and a photosensitive array; the motor and the displacement platform are both arranged in the center of the bottom plate and positioned on the same straight line; the shaft of the motor is fixedly connected with a shaft coupling through a screw, and the other end of the shaft coupling is fixedly connected to a rotating shaft of the displacement platform through a screw; a conversion part is fixed on the rotating shaft of the displacement platform, which is close to one end of the motor, and a screw on the conversion part is fixedly connected with a kit tray; one side of kit tray is equipped with a row of dentate protrusion, one side of bottom plate has L shape metalwork through the screw fixation, and L shape metalwork passes through a U-shaped photoelectric sensor of screw fixation, the both sides of bottom plate have door frame form metal rack through the screw fixation, LED array and optical alignment part are installed on the top of door frame form metal rack, and door frame form metal rack lateral wall screw fixation that is close to L shape metalwork has U-shaped photoelectric sensor, keeps away from the door frame form metal rack central point put screw fixation bin photoelectric sensor of L shape metalwork, be the sensitization array under the optical alignment part.
Preferably, the position, close to the edge of the kit tray, of the conversion piece is fixed with a stop post at the bin outlet position through threads.
Preferably, a plurality of reagent box placing areas are arranged on the reagent box tray, round holes are reserved on the reagent box placing area panel, and the round holes are distributed in an array mode.
Preferably, the L-shaped metal piece and the toothed protrusion of the kit tray are positioned on the same side.
Preferably, the U-shaped photoelectric sensor is a zero-position photoelectric sensor, and the center of the zero-position sensor and the center of the tooth-shaped bulge are positioned at the same height.
Preferably, the number of the door frame-shaped metal frames is greater than 1, and the door frame-shaped metal frames are parallel to each other.
Preferably, the U-shaped photoelectric sensor is a detection position photoelectric sensor, and the detection position photoelectric sensor and the zero position photoelectric sensor are positioned at the same height.
Preferably, the method comprises the following steps:
when the oscillation device for drug sensitivity starts to operate, the motor starts to find a zero position, when the motor drives the kit tray to move to the position of the zero position photoelectric sensor, the end of the kit tray, which is close to the motor, protrudes in a tooth shape to penetrate through the center of the zero position photoelectric sensor, so that the change of the output level of the photoelectric switch is caused, the change of the level is transmitted to the signal processing control center, the control center sends out a command to control the motor to rotate positively and negatively, and the kit tray oscillates near the zero position.
When the reagent box is needed to be placed, the signal processing control center sends a signal to the control circuit, the motor is controlled to rotate forward at a lower speed, the discharging baffle column passes through the center of the photoelectric sensor at the discharging position to cause the change of the output level of the photoelectric switch, the level change is transmitted to the signal processing control center, the control center sends an instruction to control the motor to stop rotating, the reagent box is placed to be ended, the signal processing control center sends an instruction to control the motor to move reversely, the step one is repeated, the rotating speed and the stroke of the motor during oscillation are determined by multiple experiments, and the drug sensitivity result is optimal.
And thirdly, during detection, the signal processing control center sends a signal to the control circuit, the control motor rotates positively at a lower speed, the toothed protrusion at the end far away from the motor on the kit tray passes through the center of the detection position photoelectric sensor to cause the change of the output level of the photoelectric switch, the level change is transmitted to the signal processing control center, the control center sends an instruction, the LED drive circuit drives the LED arrays at the top ends of all the metal frames to be lighted one by one in the same direction, the lighting time is determined by a plurality of experiments, the drug sensitivity result is optimal, the LED light passes through the optical collimation component to irradiate the small hole of the kit, the small hole irradiates the photosensitive array, the photosensitive array converts the received transmitted light into pulse frequency, the stronger the transmitted light is, the higher the frequency is, and the signal is transmitted to the signal processing control unit, the signal processing control unit receives the last detection data of the first row of the kit, the first row is detected and ended, the signal processing control unit continues to rotate positively at a low speed, the LED arrays adjacent to the toothed protrusion at the end far away from the motor on the kit tray passes through the center of the detection position photoelectric sensor, and the second row is detected.
And step four, after the detection of the small holes of the whole kit is finished, repeating the step one, the step two and the step three according to the time set by the system.
(III) beneficial effects
According to the oscillating device, a motor shaft is fixedly connected with a coupling through a screw, and the other end of the coupling is fixedly connected to a rotating shaft of a displacement platform through the screw. One end, close to the motor, of the displacement platform rotating shaft is fixedly connected with the kit tray through a conversion piece by a screw. A plurality of reagent box placing areas are arranged on the reagent box tray, and one end of the upright post is connected to four corners of the bottom plate through threads. The second layer can be added at the other end of the upright post according to the number of the required reagent boxes, and the structure on the second layer is the same as that of the first layer. In the whole oscillating device, a multi-layer structure is provided, each of which is controlled individually. The motor rotates positively and negatively to form an oscillating effect. The probability of the bacteria fully contacting with the medicine and oxygen in the medicine sensitization process is increased, and the time of the medicine sensitization process is shortened.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic perspective view of a drug sensitive oscillation device;
FIG. 2 is a schematic diagram of a front view of a drug sensitive oscillation device;
FIG. 3 is a control circuit diagram of the present invention;
reference numerals in the drawings represent:
1. the device comprises a bottom plate, a motor, a displacement platform, a stand column, a conversion piece, a coupling, a delivery baffle column, a 8.L-shaped metal piece, a zero-position photoelectric sensor, a door-shaped metal frame, a detection position photoelectric sensor, a delivery position photoelectric sensor, a 13-LED array, a 14-optical collimation component, a 15-photosensitive array, a 16-kit tray and a 17-kit.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As shown in fig. 1-2, the oscillating device for drug sensitivity comprises 1, a bottom plate, 2, a motor, 3, a displacement platform, 4, an upright post, 5, a conversion piece, 6, a coupling, 7, a discharging baffle post, 8, an L-shaped metal piece, 9, a zero photoelectric sensor, 10, a door-shaped metal frame, 11, a detection position photoelectric sensor, 12, a discharging position photoelectric sensor, 13, an LED array, 14, an optical collimation part, 15, a photosensitive array, 16 and a kit tray. The motor 2 and the displacement platform 3 are arranged in the center of the bottom plate 1 and positioned on the same straight line. The motor shaft is fixedly connected with a coupling 6 through a screw, and the other end of the coupling 6 is fixedly connected to a rotating shaft of the displacement platform 3 through the screw. One end, close to the motor 2, of the rotating shaft of the displacement platform 3 is fixed with a conversion piece 5, and a screw on the conversion piece 5 is fixedly connected with a kit tray 16. The position of the edge of the conversion piece 5, which is close to the reagent box tray 16, is fixed with a bin stop post 7 through threads. The reagent box tray 16 is provided with a plurality of reagent box placing areas, the size of the reagent box placing areas is determined according to the size of the reagent box, a plurality of round holes are reserved on the panel of the reagent box placing areas and distributed in an array shape, and the number, the size and the distance between the small holes of the round holes are determined by the reagent box. One side of the kit tray 16 has a row of teeth-like projections, the number of teeth-like projections being determined by the number of rows of wells in the kit. The distance between the teeth is determined by the distance between the centers of the adjacent rows of wells in the kit. One side of the bottom plate is fixed with an L-shaped metal piece 8 through a screw, and the L-shaped metal piece and the toothed bulge of the reagent box tray 16 are positioned on the same side. A U-shaped photoelectric sensor is fixed at a certain height of the L-shaped metal piece 8 through a screw, and the U-shaped photoelectric sensor is a zero photoelectric sensor 9. The center of the zero sensor 9 is at the same height as the center of the tooth-like projections. The two sides of the bottom plate are fixed with a plurality of door frame-shaped metal frames 10 by screws, and the number of the door frame-shaped metal frames 10 is determined by the number of rows of the reagent box placing areas on the reagent box tray 16. The plurality of doorframe-shaped metal frames 10 are parallel to each other. An LED array 13 and an optical collimator 14 are mounted on the top end of the doorframe-like metal frame 10. A U-shaped photoelectric sensor is fixed on the side wall of the doorframe-shaped metal frame 10 adjacent to the L-shaped metal piece 8 through screws, and the detection position photoelectric sensor 11 and the zero position photoelectric sensor 9 are positioned at the same height for detecting the position photoelectric sensor 11. The U-shaped photoelectric sensor is fixed at the center of the doorframe-shaped metal frame 10 far away from the L-shaped metal frame 8 by screws, and is a photoelectric sensor 12 at the warehouse-out position. Directly below the optical collimating component 14 is a photosensitive array 15.
The method for controlling the oscillation device for drug sensitivity comprises the oscillation control device for drug sensitivity and further comprises the following steps.
With reference to fig. 1-3.
When the oscillation device for drug sensitivity starts to operate, the motor 2 starts to find a zero position, when the motor 2 drives the reagent box tray 16 to move to the position of the zero position photoelectric sensor 9, the tooth-shaped protrusions on the reagent box tray 16, which are close to the motor 2, penetrate through the center of the zero position photoelectric sensor 9 to cause the photoelectric sensor to change from a high level to a low level, the level change is transmitted to a pin P1.3 of the signal processing control center, the control center sends out an instruction, a high-low level signal is sent out through the pin P1.0 to control the direction of the motor, a pulse signal is sent out through the pin P1.2 to control the rotating speed of the motor, and the reagent box tray oscillates near the zero position.
When the reagent kit 17 needs to be placed, the signal processing control center sends out a low-frequency pulse signal through P1.2, sends out a high-level signal through P1.0, controls the motor 2 to rotate forward at a lower speed, the discharging baffle column 7 passes through the center of the discharging photoelectric sensor 12, the output level of the photoelectric sensor 12 is caused to be changed from a high level to a low level, the level change is transmitted to the signal processing control center, the signal processing control center sends out an instruction, the sending out of the pulse signal is stopped through P1.2, and the motor 2 stops rotating. And after the reagent box 17 is placed, the signal processing control center sends out a command, P1.0 sends out a low-level signal, P1.2 sends out a low-frequency pulse signal, and the motor 2 is controlled to reversely rotate at a lower speed to control the motor 2 to reversely move. Repeating the first step. The rotating speed and the stroke of the motor 2 during oscillation are determined by multiple experiments and the optimal drug sensitivity result.
And thirdly, during detection, the signal processing control center sends out a signal, P1.2 sends out a low-frequency pulse signal, P1.0 sends out a high-level signal, the motor 2 is controlled to rotate positively at a lower speed, the reagent box tray 17 is far away from the center of the detection position photoelectric sensor 11 and protrudes in a tooth shape, the output level of the detection position photoelectric sensor 11 is changed from high to low, the level change is transmitted to the signal processing control center, the control center sends out an instruction, the LED driving circuit drives the LED arrays at the top ends of all the metal frames to be lighted one by one in the same direction, the lighting time is determined by multiple experiments, the drug sensitivity result is optimal, the LED light passes through the optical collimation component to be irradiated to small holes of the reagent box and passes through the small holes to be irradiated to the photosensitive array, and the photosensitive array converts received transmitted light into pulse frequency, so that the stronger transmitted light is higher in frequency. And transmits the signal to the signal processing control unit. The signal processing control unit receives the last detection data of the first row of the kit, and the detection of the first row is finished. The signal processing control unit continues to rotate forward at a low speed, and the protrusion adjacent to the toothed protrusion far away from the motor end on the kit tray passes through the center of the photoelectric sensor at the detection position. The second row starts to be detected.
And step four, finishing the detection of the whole kit small hole. And repeating the first, second and third steps according to the time set by the system.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
The above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (6)
1. An oscillation device for drug sensitivity comprises a bottom plate, a motor, a displacement platform and an upright post; the method is characterized in that: the device also comprises a photoelectric sensor, an optical detection device, a control circuit and a signal processing control unit, wherein the optical detection device comprises a white light LED array, an LED driving circuit, an optical collimation component and a photosensitive array; the motor and the displacement platform are both arranged in the center of the bottom plate and positioned on the same straight line; the shaft of the motor is fixedly connected with a shaft coupling through a screw, and the other end of the shaft coupling is fixedly connected to a rotating shaft of the displacement platform through a screw; a conversion part is fixed on the rotating shaft of the displacement platform, which is close to one end of the motor, and a screw on the conversion part is fixedly connected with a kit tray; a row of tooth-shaped protrusions are arranged on one side of the kit tray, an L-shaped metal piece is fixed on one side of the bottom plate through a screw, a U-shaped photoelectric sensor is fixed on the L-shaped metal piece through the screw, and the U-shaped photoelectric sensor is a zero photoelectric sensor; the LED light source is characterized in that two sides of the bottom plate are fixed with a door frame-shaped metal frame through screws, the top end of the door frame-shaped metal frame is provided with an LED array and an optical alignment part, a U-shaped photoelectric sensor is fixed on the side wall of the door frame-shaped metal frame close to the L-shaped metal piece through screws, and the U-shaped photoelectric sensor is a detection position photoelectric sensor; a photoelectric sensor of a delivery position is fixed at the center of the doorframe-shaped metal frame far away from the L-shaped metal frame by a screw, and a photosensitive array is arranged right below the optical alignment component; the position, close to the edge of the kit tray, of the conversion piece is fixed with a delivery baffle column through threads;
the control method of the oscillation device comprises the following steps:
when the oscillation device for drug sensitivity starts to operate, a motor starts to find a zero position, and when the motor drives a reagent box tray to move to the position of a zero position photoelectric sensor, the reagent box tray protrudes through the center of the zero position photoelectric sensor in a tooth shape near the end of the motor to cause the change of the output level of a photoelectric switch, the level change is transmitted to a signal processing control center, the control center sends out a command to control the motor to rotate positively and negatively, and the reagent box tray oscillates near the zero position;
when the reagent box is required to be placed, the signal processing control center sends a signal to the control circuit, the motor is controlled to rotate forward at a low speed, the discharging baffle column passes through the center of the photoelectric sensor at the discharging position to cause the change of the output level of the photoelectric switch, the level change is transmitted to the signal processing control center, the control center sends an instruction to control the motor to stop rotating, the reagent box is placed, the signal processing control center sends an instruction to control the motor to move reversely, the step one is repeated, the rotating speed and the stroke of the motor during oscillation are determined by multiple experiments, and the drug sensitivity result is optimal;
step three, during detection, the signal processing control center sends a signal to the control circuit, the motor is controlled to rotate forward at a low speed, the toothed protrusion at the end far away from the motor on the reagent box tray passes through the center of the photoelectric sensor at the detection position, the change of the output level of the photoelectric switch is caused, the level change is transmitted to the signal processing control center, the control center sends an instruction, the LED drive circuit drives the LED arrays at the top ends of all the metal frames to be lighted one by one in the same direction, the lighting time is determined by a plurality of experiments, the drug sensitivity result is optimal, the LED light passes through the optical collimating component to irradiate the small hole of the reagent box, the small hole irradiates the photosensitive array, the photosensitive array converts the received transmitted light into pulse frequency, the stronger the transmitted light is, the higher the frequency is, and the signal is transmitted to the signal processing control unit, the signal processing control unit receives the last detection data of the first row of the reagent box, the detection of the first row is finished, the signal processing control unit continues the low-speed forward rotation, the protrusions adjacent to the toothed protrusion at the end far away from the motor on the reagent box tray pass through the center of the photoelectric sensor at the detection position, and the second row starts to be detected;
and step four, after the detection of the small holes of the whole kit is finished, repeating the step one, the step two and the step three according to the time set by the system.
2. The oscillating device for drug sensitivity of claim 1, wherein: the kit tray is provided with a plurality of kit placement areas, round holes are reserved on the panel of the kit placement areas, and the round holes are distributed in an array mode.
3. The oscillating device for drug sensitivity of claim 1, wherein: the L-shaped metal piece and the toothed bulge of the kit tray are positioned on the same side.
4. The oscillating device for drug sensitivity of claim 1, wherein: the center of the zero photoelectric sensor and the center of the tooth-shaped bulge are positioned at the same height.
5. The oscillating device for drug sensitivity of claim 1, wherein: the number of the door frame-shaped metal frames is larger than 1, and the door frame-shaped metal frames are mutually parallel.
6. The oscillating device for drug sensitivity of claim 1, wherein: the detection position photoelectric sensor and the zero position photoelectric sensor are located at the same height.
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