CN106754315A - A kind of reaction unit for simulating animal rumens fermentation - Google Patents
A kind of reaction unit for simulating animal rumens fermentation Download PDFInfo
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- CN106754315A CN106754315A CN201710111695.4A CN201710111695A CN106754315A CN 106754315 A CN106754315 A CN 106754315A CN 201710111695 A CN201710111695 A CN 201710111695A CN 106754315 A CN106754315 A CN 106754315A
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- fermentation tank
- fermentation
- reaction unit
- pipe
- bottle stopper
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/08—Stirrer or mobile mixing elements with different stirrer shapes in one shaft or axis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- Life Sciences & Earth Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Sustainable Development (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
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- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention provides a kind of reaction unit for simulating animal rumens fermentation, including fermentation tank, bottle stopper is provided with the top of the fermentation tank, bottle stopper is provided with feeding-in solid body pipe, liquid feed pipe, rotary shaft and escaping pipe on the bottle stopper, described rotary shaft one end connects motor, the other end stretches into fermentation tank inside and its end connection bottom blade, the top for stretching into the rotary shaft inside fermentation tank is connected with top blade, the bottom of the fermentation tank is provided with air inlet pipe, and the top of the fermentation tank is provided with drain pipe.The reaction unit uses mechanical agitation mode, truly simulates rumen peristalsis situation, can be with automatic feeding and discharging, it is easy to analyze solid-like and liquid-like, while air-tightness is good, can effectively analyzes the gas produced in fermentation process, simple structure, it is simple to operate, it is easy to safeguard and change part.
Description
Technical field
The invention belongs to animal nutrition technical field, and in particular to a kind of reaction dress for simulating animal rumens fermentation
Put, can be used to evaluate the effect of lumen fermentation.
Background technology
Ruminate, refer in again returning to chew in mouth and return stomach by the food of half digestion in stomach after a period of time on the feed
Phenomenon, ruminate and have great importance for some herbivores.Cud, is the first stomach of ruminant, is one natural
Degradation of fibers material fermentation tank, the research to ruminating phenomenon be conducive to deeply understand ruminant nutrition feature.
At present, typically there are three kinds of technique study lumen fermentations, the first is viviperception, i.e., in selected animal stomach hand
Art drills, and feed is put into wherein, regular sampling analysis；Second is that fresh cud is removed and placed in a container, past
Feed is put into cud, the digestion characteristics of feed are studied；The third is simulated experiment, by artificial saliva, each quasi-microorganism etc. and
Feed mixes, the simulated experiment in a fermentation tank.Wherein, first method most authenticity, data is also reliable, but
Cost highest, and operation difficulty is larger；Second method is reliable, but the usage cycles of fresh cud are shorter, to parallel
Sample requirement is relatively more；The third method operates relative ease, it is adaptable to high-volume analog study.But, it is dynamic currently used for simulation
The reaction unit of thing lumen fermentation is rarely found, and indivedual existing devices have stirring can not true simulation rumen wriggling situation, no
The problems such as air-tightness is good can simultaneously be accomplished.
The content of the invention
The purpose of the present invention be overcome existing lumen fermentation stirring can not truly simulation rumen wriggling situation, can not be simultaneously
Accomplish the good problem of air-tightness.
Therefore, the invention provides a kind of reaction unit for simulating animal rumens fermentation, including fermentation tank, it is described
Bottle stopper is provided with the top of fermentation tank, on the bottle stopper through bottle stopper be provided with feeding-in solid body pipe, liquid feed pipe, rotary shaft and
Escaping pipe, described rotary shaft one end connects motor, and the other end stretches into fermentation tank inside and its end connection bottom blade, stretches into
The top of the rotary shaft inside fermentation tank is connected with top blade, and the bottom of the fermentation tank is provided with air inlet pipe, institute
The top for stating fermentation tank is provided with drain pipe.
Further, it is provided with seal between the rotary shaft and bottle stopper.
Further, the seal includes outer shroud and inner ring, and the inner ring madial wall is circumferentially arranged with sealed groove, institute
State and seal grease is filled in sealed groove.
Further, the feeding-in solid body pipe, between liquid feed pipe, escaping pipe, air inlet pipe and bottle stopper by sealing
Glue is sealed.
Further, the fermentation tank is internally provided with the string bag.
Further, it is provided with mesh screen on the drain pipe.
Further, the top blade be arranged in parallel with fermentation tank bottom, and bottom blade is in fermentation tank bottom
45° angle is set, and bottom blade is approached with fermentation tank bottom.
Further, the reaction unit for being used to simulate animal rumens fermentation also includes Water Tank with Temp.-controlled, the fermentation tank
It is placed in the Water Tank with Temp.-controlled.
Further, the reaction unit for being used to simulate animal rumens fermentation also includes gas flowmeter and gas analysis
Instrument, the escaping pipe is sequentially connected gas flowmeter and gas analyzer.
Compared with prior art, beneficial effects of the present invention：
(1) this reaction unit for simulating animal rumens fermentation that the present invention is provided is using mechanical agitation mode, true mould
Rumen peristalsis situation is intended, while can be easy to analyze solid-like and liquid-like with automatic feeding and discharging.
(2) this air-tightness for simulating the reaction unit that animal rumens ferment that the present invention is provided is good, Ke Yiyou
The gas produced in effect analysis fermentation process.
(3) what the present invention was provided is this simple to operate for simulating the reaction unit of animal rumens fermentation, easy to use, instead
Apparatus moduleization is answered to design, it is easy to change part.
The present invention is described in further details below with reference to accompanying drawing.
Brief description of the drawings
Fig. 1 is the present invention for simulating the structural representation of the reaction unit of animal rumens fermentation；
Fig. 2 is the structural representation of seal in the present invention.
Description of reference numerals：1st, fermentation tank；2nd, the string bag；3rd, drain pipe；4th, mesh screen；5th, bottle stopper；6th, gas analyzer；7、
Gas flowmeter；8th, escaping pipe；9th, motor；10th, rotary shaft；11st, seal；12nd, liquid feed pipe；13rd, feeding-in solid body pipe；
14th, air inlet pipe；15th, top blade；16th, bottom blade；17th, Water Tank with Temp.-controlled；18th, outer shroud；19th, inner ring, 20, sealed groove.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is all other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
As shown in figure 1, a kind of reaction unit for simulating animal rumens fermentation is present embodiments provided, including fermentation tank
Body 1, the top of the fermentation tank 1 is provided with bottle stopper 5, and bottle stopper 5 is provided with feeding-in solid body pipe 13, liquid on the bottle stopper 5
Feed pipe 12, rotary shaft 10 and escaping pipe 8, the one end of the rotary shaft 10 connection motor 9, the other end are stretched into inside fermentation tank 1
And its end connects bottom blade 16, the top for stretching into the rotary shaft 10 inside fermentation tank 1 is connected with top blade 15,
The bottom of the fermentation tank 1 is provided with air inlet pipe 14, and the top of the fermentation tank 1 is provided with drain pipe 3.Wherein, bottle stopper 5 is by having
Resilient rubber processing is formed, and sealing function can be played by self-deformation.
Before simulation animal rumens fermenting experiment starts, first solid material to be fermented and microorganism fungus kind are mixed to prepare and put
Enter in fermentation tank 1, then the rotary shaft 10 for connecting motor 9 is inserted into the centre bore of bottle stopper 5, then by the He of top blade 15
Bottom blade 16 passes sequentially through screw and is fixed in rotary shaft 10, and bottle stopper 5 is finally buckled into the bottleneck of fermentation tank 1.Preferably,
O-ring is provided with bottle stopper 5, O-ring is squeezed generation deformation so as to play sealing function when bottle stopper 5 buckles into fermentation tank 1.
During simulation animal rumens fermenting experiment, oxygenless gas such as nitrogen or carbon dioxide are passed through toward air inlet pipe 14 is interior, will
Oxygen in fermentation tank 1 is driven out of from escaping pipe 8, is then shut off air inlet pipe 14 and escaping pipe 8, builds anaerobic fermentation environment；Open
Motor 1 is opened, the rotating speed such as 1~20rpm of corresponding rotary shaft 10 is set, simulation rumen wriggling situation, motor 1 is rotated axle 10
Rotate, rotary shaft 10 drives top blade 15 and bottom blade 16 to rotate, uniform stirring is played a part of to reaction mass again.It is excellent
Choosing, the top blade 15 be arranged in parallel with the bottom of fermentation tank 1, is conducive to the agitation upper space of fermentation tank 1 and right
The issuable foam that ferments plays shearing elimination effect, prevents foam from blocking escaping pipe 8；The bottom blade 16 and fermentation tank
The bottom of body 1 is set in 45° angle, and bottom blade 16 is approached with the bottom of fermentation tank 1, can easily stir fermentation tank 1
The reaction mass of bottom, prevents reaction mass from depositing.
And for more real simulation rumen fermentation situation, fermentation tank 1 can be placed in Water Tank with Temp.-controlled 17, by perseverance
Warm water tank 17 sets such as 39 DEG C of temperature needed for lumen fermentation.In experimentation, solids can be added toward feeding-in solid body pipe 13
Material, also can add liquid material from liquid feed pipe 12, can take liquid-like from drain pipe 3, realize automatic feeding and discharging, just
In analysis solid-like and liquid-like.
In addition, in experimentation, the liquid-like taken out from drain pipe 3 can be further analyzed, by escaping pipe 8 and gas
Flowmeter 7 and gas analyzer 6 are sequentially connected, can be with test experiments process gas production and gas constituent；After experiment terminates,
Remaining solid sample in fermentation tank 1 can be collected to be analyzed.
After experiment terminates, the switch of Water Tank with Temp.-controlled 17 and motor 9 are closed successively, unplug bottle stopper 5, take apart upper in rotary shaft 10
Portion's blade 15 and bottom blade 16, are cleaned respectively, standby.
Preferred embodiment, in order to further improve the sealing of reaction unit of the present invention, the rotary shaft 10 and bottle
Seal 11 is provided between plug 5, the structure of the seal 11 is as shown in Fig. 2 the seal 11 includes outer shroud 18 and inner ring
19, the madial wall of the inner ring 19 fills seal grease circumferentially arranged with sealed groove 20 in the sealed groove 20.Seal 11
Buckle at the center drilling of bottle stopper 5, the outer shroud 18 of seal 11 is brought into close contact with the center drilling inwall of bottle stopper 5, by bottle stopper 5
Stress produces deformation to play sealing function；Rotary shaft 10 is inserted in the inner ring 19 of seal 11 again, toward the inner ring of seal 11
Seal grease is added in 19 sealed groove 20, rotation is worked as in the gap between filling rotary shaft 10 and the inner ring 19 of seal 11
When axle 10 is rotated, seal grease can be formed between liquid film is filled in the gap of the inner ring 19 of rotary shaft 10 and seal 11 and play close
Envelope is acted on, while playing lubrication.Meanwhile, by the feeding-in solid body pipe 13, liquid feed pipe 12, escaping pipe 8, air inlet pipe 14
Pass through sealant sealing between bottle stopper 5.
In addition, after terminating for the ease of reaction, can easily take out the remaining solid material of reaction carry out it is follow-up
Weigh analysis, the fermentation tank 1 is internally provided with the string bag 2, before experiment starts, by solid material and microbial bacteria to be fermented
Kind being put into the string bag 2 carries out fermentation reaction, and the string bag 2 is directly taken out collection remaining solid sample and is analyzed by experiment after terminating.
And in order to avoid in fermentation tank 1 solid residue flowed out from drain pipe 3 with liquid-like, block drain pipe 3 or influence liquid-like point
Analysis result, is provided with mesh screen 4, by solid residue in the filter liquid sample of mesh screen 4 on the drain pipe 3.
Contrasted with live body lumen fermentation for simulating the reaction unit of animal rumens fermentation using of the invention, its
In, the present invention is used to set 39 DEG C of reaction temperature in the reaction unit for simulate animal rumens fermentation, the rpm of 1 mixing speed of motor 5,
Anaerobic state is good in fermentation tank, and air-tightness is fine.Result shows, measures liquid-like pH value 6.9, ammoniacal nitrogen in fermentation tank
Content 12（mg/100 mL）, bacterial number 30*109Individual/mL, protozoon quantity 15*103Individual/mL, general volatile 90 mmol of acid,
Acetic acid, propionic acid, butyric acid and valeric acid ratio are respectively 63%, 20%, 8% and 2%, organic matter digestibility 57%.With live body lumen fermentation
Experimental data is compared, and is substantially conformed to, it was demonstrated that this device is applied to simulation animal rumens fermentation reaction.
In sum, this reaction unit for simulating animal rumens fermentation that the present invention is provided is using mechanical agitation side
Formula, truly simulates rumen peristalsis situation, can be easy to analyze solid-like and liquid-like, while airtight with automatic feeding and discharging
Property it is good, can effectively analyze the gas produced in fermentation process, simple structure is simple to operate, it is easy to safeguard and change part.
It is exemplified as above be only to of the invention for example, do not constitute the limitation to protection scope of the present invention, it is all
It is that design same or analogous with the present invention is belonged within protection scope of the present invention.
Claims (9)
1. a kind of reaction unit for simulating animal rumens fermentation, including fermentation tank (1), it is characterised in that：The fermentation
Bottle stopper (5) is provided with the top of tank body (1), bottle stopper (5) is provided with feeding-in solid body pipe (13), liquid on the bottle stopper (5) enters
Expects pipe (12), rotary shaft (10) and escaping pipe (8), described rotary shaft (10) one end connect motor (9), and the other end stretches into fermentation tank
Body (1) is internal and its end connects bottom blade (16), and the top for stretching into the internal rotary shaft (10) of fermentation tank (1) connects
Top blade (15) is connected to, the bottom of the fermentation tank (1) is provided with air inlet pipe (14), and the top of the fermentation tank (1) sets
There is drain pipe (3).
It is 2. as claimed in claim 1 to be used to simulate the reaction unit that animal rumens ferment, it is characterised in that：The rotary shaft
(10) seal (11) is provided with and bottle stopper (5) between.
It is 3. as claimed in claim 2 to be used to simulate the reaction unit that animal rumens ferment, it is characterised in that：The seal
(11) including outer shroud (18) and inner ring (19), inner ring (19) madial wall is circumferentially arranged with sealed groove (20), the sealing
Seal grease is filled in groove (20).
It is 4. as claimed in claim 1 to be used to simulate the reaction unit that animal rumens ferment, it is characterised in that：The feeding-in solid body
Manage (13), pass through sealant sealing between liquid feed pipe (12), escaping pipe (8), air inlet pipe (14) and bottle stopper (5).
It is 5. as claimed in claim 1 to be used to simulate the reaction unit that animal rumens ferment, it is characterised in that：The fermentation tank
(1) it is internally provided with the string bag (2).
It is 6. as claimed in claim 1 to be used to simulate the reaction unit that animal rumens ferment, it is characterised in that：The drain pipe
(3) mesh screen (4) is provided with.
It is 7. as claimed in claim 1 to be used to simulate the reaction unit that animal rumens ferment, it is characterised in that：The top blade
(15) it is be arranged in parallel with fermentation tank (1) bottom, bottom blade (16) is set with fermentation tank (1) bottom in 45° angle, and bottom
Blade (16) is approached with fermentation tank (1) bottom.
8. as described in any one of claim 1~7 for simulate animal rumens fermentation reaction unit, it is characterised in that：Also
Including Water Tank with Temp.-controlled (17), the fermentation tank (1) is placed in the Water Tank with Temp.-controlled (17).
It is 9. as claimed in claim 8 to be used to simulate the reaction unit that animal rumens ferment, it is characterised in that：Also include gas stream
Gauge (7) and gas analyzer (6), the escaping pipe (8) are sequentially connected gas flowmeter (7) and gas analyzer (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710111695.4A CN106754315A (en) | 2017-02-28 | 2017-02-28 | A kind of reaction unit for simulating animal rumens fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710111695.4A CN106754315A (en) | 2017-02-28 | 2017-02-28 | A kind of reaction unit for simulating animal rumens fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106754315A true CN106754315A (en) | 2017-05-31 |
Family
ID=58960003
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710111695.4A Withdrawn CN106754315A (en) | 2017-02-28 | 2017-02-28 | A kind of reaction unit for simulating animal rumens fermentation |
Country Status (1)
| Country | Link |
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| CN (1) | CN106754315A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111899622A (en) * | 2020-09-16 | 2020-11-06 | 广州市富泉生物科技有限公司 | Method for measuring rumen-bypass release rate by in-vitro simulation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1370826A (en) * | 2001-02-27 | 2002-09-25 | 中国农业大学 | Double outer-flow type fermenter for simulating rumen fermentation in laboratory |
| CN102286359A (en) * | 2010-12-28 | 2011-12-21 | 中国农业科学院北京畜牧兽医研究所 | Solid-liquid-gas three phase flow-dividing rumen imitation continuous fermentation system and method |
-
2017
- 2017-02-28 CN CN201710111695.4A patent/CN106754315A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1370826A (en) * | 2001-02-27 | 2002-09-25 | 中国农业大学 | Double outer-flow type fermenter for simulating rumen fermentation in laboratory |
| CN102286359A (en) * | 2010-12-28 | 2011-12-21 | 中国农业科学院北京畜牧兽医研究所 | Solid-liquid-gas three phase flow-dividing rumen imitation continuous fermentation system and method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111899622A (en) * | 2020-09-16 | 2020-11-06 | 广州市富泉生物科技有限公司 | Method for measuring rumen-bypass release rate by in-vitro simulation |
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Application publication date: 20170531 |
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| WW01 | Invention patent application withdrawn after publication |