CN106754310A - 一种dna聚合酶试剂盒 - Google Patents

一种dna聚合酶试剂盒 Download PDF

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Publication number
CN106754310A
CN106754310A CN201611234537.XA CN201611234537A CN106754310A CN 106754310 A CN106754310 A CN 106754310A CN 201611234537 A CN201611234537 A CN 201611234537A CN 106754310 A CN106754310 A CN 106754310A
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Prior art keywords
dna polymerase
archaeal dna
polymerase kit
antimicrobial coating
aziridine
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刘喜朋
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Suzhou Kuangshi Junchi Biological Science & Technology Co Ltd
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Suzhou Kuangshi Junchi Biological Science & Technology Co Ltd
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Priority to CN201611234537.XA priority Critical patent/CN106754310A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

本发明公开了一种DNA聚合酶试剂盒，包括上盖、盒体、支架，所述盒体外表面涂有一层抗菌涂层；所述抗菌涂层各成分质量比为：草木灰10~20、二硫化钼5~10、尿素50~100、丙烯酸酯乳液250~300、邻苯二甲酸二辛酯10~50、氮化镁5~10、氧化锌2~3、氮丙啶3~4；将上述成分混合均匀后，加入氨水适量，调PH值为7±0.5。本发明一种DNA聚合酶试剂盒对空气中杂菌有抑制作用，增加了PCR试验数据准确性。

Description

一种DNA聚合酶试剂盒
技术领域
本发明涉及一种DNA聚合酶试剂盒。
背景技术
市面上的DNA聚合酶试剂盒在实用时存在被空气中杂菌污染的情况，为了减少空气中杂菌对PCR试验数据的影响，需要在DNA聚合酶试剂盒表面涂层抗菌涂层。
发明内容
本发明目的在于提供一种DNA聚合酶试剂盒，旨在解决上述问题。
为了实现上述目的，本发明的技术方案是设计一种DNA聚合酶试剂盒，包括上盖、盒体、支架，所述盒体外表面涂有一层抗菌涂层；所述抗菌涂层各成分质量比为：草木灰10~20、二硫化钼5~10、尿素50~100、丙烯酸酯乳液250~300、邻苯二甲酸二辛酯10~50、氮化镁5~10、氧化锌2~3、氮丙啶3~4；将上述成分混合均匀后，加入氨水适量，调PH值为7±0.5。
本发明的优点和有益效果在于：本发明一种DNA聚合酶试剂盒对空气中杂菌有抑制作用，增加了PCR试验数据准确性。
具体实施方式
下面结合实施例，对本发明的具体实施方式作进一步描述。以下实施例仅用于更加清楚地说明本发明的技术方案，而不能以此来限制本发明的保护范围。
实施例1：
一种DNA聚合酶试剂盒，所述抗菌涂层各成分质量比为：草木灰20、二硫化钼5、尿素55、丙烯酸酯乳液300、邻苯二甲酸二辛酯45、氮化镁7、氧化锌3、氮丙啶3.5；将上述成分混合均匀后，加入氨水适量，调PH值为7±0.5。
实施例2;
一种DNA聚合酶试剂盒，所述抗菌涂层各成分质量比为：草木灰10、二硫化钼7、尿素100、丙烯酸酯乳液280、邻苯二甲酸二辛酯45、氮化镁10、氧化锌3、氮丙啶4；将上述成分混合均匀后，加入氨水适量，调PH值为7±0.5。
以上所述仅是本发明的优选实施方式，应当指出，对于本技术领域的普通技术人员来说，在不脱离本发明技术原理的前提下，还可以做出若干改进和润饰，这些改进和润饰也应视为本发明的保护范围。

Claims (3)

1.一种DNA聚合酶试剂盒，包括上盖、盒体、支架，其特征在于：所述盒体外表面涂有一层抗菌涂层；所述抗菌涂层各成分质量比为：草木灰10~20、二硫化钼5~10、尿素50~100、丙烯酸酯乳液250~300、邻苯二甲酸二辛酯10~50、氮化镁5~10、氧化锌2~3、氮丙啶3~4；将上述成分混合均匀后，加入氨水适量，调PH值为7±0.5。
2.根据权利要求1所述的一种DNA聚合酶试剂盒，其特征在于：所述抗菌涂层各成分质量比为：草木灰20、二硫化钼5、尿素55、丙烯酸酯乳液300、邻苯二甲酸二辛酯45、氮化镁7、氧化锌3、氮丙啶3.5；将上述成分混合均匀后，加入氨水适量，调PH值为7±0.5。
3.根据权利要求1所述的一种DNA聚合酶试剂盒，其特征在于：所述抗菌涂层各成分质量比为：草木灰10、二硫化钼7、尿素100、丙烯酸酯乳液280、邻苯二甲酸二辛酯45、氮化镁10、氧化锌3、氮丙啶4；将上述成分混合均匀后，加入氨水适量，调PH值为7±0.5。
CN201611234537.XA 2016-12-28 2016-12-28 一种dna聚合酶试剂盒 Pending CN106754310A (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109182300A (zh) * 2018-08-17 2019-01-11 江苏大学 一种抑制dna酶切的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104327769A (zh) * 2014-09-30 2015-02-04 河南中包科技有限公司 一种防霉耐水易碱洗性能优良的环保标签粘合剂及其制法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104327769A (zh) * 2014-09-30 2015-02-04 河南中包科技有限公司 一种防霉耐水易碱洗性能优良的环保标签粘合剂及其制法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109182300A (zh) * 2018-08-17 2019-01-11 江苏大学 一种抑制dna酶切的方法
CN109182300B (zh) * 2018-08-17 2021-10-12 江苏大学 一种抑制dna酶切的方法

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