CN106754301A - A kind of high flux steel ball loading methods and its device - Google Patents
A kind of high flux steel ball loading methods and its device Download PDFInfo
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- CN106754301A CN106754301A CN201710024135.5A CN201710024135A CN106754301A CN 106754301 A CN106754301 A CN 106754301A CN 201710024135 A CN201710024135 A CN 201710024135A CN 106754301 A CN106754301 A CN 106754301A
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- sieve plate
- steel ball
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
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Abstract
The invention discloses a kind of high flux steel ball loading methods and its device, the middle sieve plate of the device is respectively provided on two sides with protrusive board, and described protrusive board inserts in the perforate of base side wall, middle sieve plate is fixed between upper and lower sieve plate；2 rectangular v notch vs are provided with the protrusive board side of middle sieve plate, the first spring, second spring are located in breach, are connected with the side wall of pedestal；Upper, middle and lower sieve plate is uniformly provided with 96 holes of 8 row × 12 row, upper, 96 hole alignments of middle sieve plate, in, 96 hole Heterogeneous Permutations of lower sieve plate, 96 holes for making middle sieve plate by the protrusive board on sieve plate in promotion are alignd with the hole of lower sieve plate.When using, grinding steel ball is poured into upper sieve plate top, the raised each pipe that just can be realized once to 96 hole DNA extraction tubes in sieve plate outside adds a grinding steel ball in the middle of by promoting, adding when the method not only avoids traditional-handwork plus steel ball or leak plus, more effectively improve experimenter extract DNA efficiency.
Description
Technical field
The invention belongs to Bioexperiment device field, and in particular to a kind of high flux steel ball sample adding device, one kind is further related to
The method of high flux steel ball sample-adding, for adding grinding steel ball to centrifuge tube batch in DNA extraction process.
Background technology
In biological experiment, needed historrhexises such as blade, seeds when extracting animal vegetable tissue genomic DNA, led to
Normal way is that experiment material is loaded into centrifuge tube, then adds a steel ball, is placed on and grinding is shaken on tissue grinder instrument；Due to centrifugation
Pipe diameter is small, need to be ground with small steel ball, in process of lapping, adds steel ball to be easy to occur leaking add or add manually, and take
Long, inefficiency, have impact on DNA extraction efficiencies, it is therefore necessary to develop a kind of to adding grinding steel ball in centrifuge tube in batches
Device and method.
The content of the invention
It is an object of the invention to provide a kind of high flux steel ball sample adding device, simple structure, it is to avoid traditional-handwork adds
Easily occurs adding or leaking the problem for adding during steel ball.
It is another object of the present invention to provide a kind of high flux steel ball loading methods, in DNA extraction process to
Centrifuge tube batch adds grinding steel ball, improves DNA extraction efficiencies.
To achieve the above object, the present invention takes following technical scheme：
A kind of high flux steel ball sample adding device, including pedestal, upper sieve plate, middle sieve plate, lower sieve plate, it is characterised in that：Pedestal
It is the cuboid of upper and lower opening, upper sieve plate and lower sieve plate are fixedly connected with the side wall of pedestal respectively, the length of middle sieve plate is less than base
The length of seat, middle sieve plate is respectively provided on two sides with protrusive board, in the perforate of described protrusive board insertion base side wall, middle sieve plate is fixed
Between upper and lower sieve plate；2 rectangular v notch vs are provided with the protrusive board side of middle sieve plate, the first spring, second spring are located at breach
In, described spring side wall respectively with pedestal, middle sieve plate are connected；Upper, middle and lower sieve plate is uniformly provided with 96 holes of 8 row × 12 row,
96 hole Heterogeneous Permutations of 96 hole alignments of upper sieve plate and middle sieve plate, middle sieve plate and lower sieve plate, by sieve plate in promotion
Protrusive board makes 96 holes of middle sieve plate be alignd with the hole of lower sieve plate.
The depth in 96 holes of upper sieve plate and middle sieve plate is equal to the diameter of steel ball, upper sieve plate, middle sieve plate, 96 holes of lower sieve plate
Aperture is equal to diameter of the steel ball.
Upper sieve plate and lower sieve plate are respectively equipped with protrusive board, and described protrusive board is inserted in the perforate of pedestal.
A kind of high flux steel ball loading methods, comprise the following steps：
A. 96 holes by the centrifuge tube of 96 orifice plates of DNA extraction elements respectively with lower sieve plate are alignd；
B., grinding steel ball is positioned over the surface of upper sieve plate, swaying base makes steel ball roll into 96 holes of upper sieve plate automatically
It is interior；
C. the protrusive board of sieve plate makes 96 holes of middle sieve plate and lower sieve plate align in promoting, and now the steel ball in middle sieve plate hole is automatic
Fall into the hole of lower sieve plate, and then fall into centrifuge tube；
D. sieve plate in being unclamped after the completion of steel ball is added, middle sieve plate resets under the elastic reaction of spring, and each Kong Jun
Alignd with the hole of upper sieve plate, the steel ball of upper sieve plate can be again introduced into middle sieve plate hole again.
Compared with prior art, the present invention has advantages below and beneficial effect：
Grinding steel ball is poured into pedestal top, just can be realized once to 96 holes by protrusive board on the outside of sieve plate in the middle of push-and-pull
Each pipe of DNA extraction tubes adds a grinding steel ball, adding when the method not only avoids tradition hand plus steel ball or
Leakage adds, and more effectively improves the efficiency that experimenter extracts DNA.
Brief description of the drawings
Fig. 1 is the structural representation of high flux steel ball sample adding device；
Fig. 2 is the pedestal of high flux steel ball sample adding device；
Fig. 3 is upper sieve plate, middle sieve plate, the schematic diagram of lower sieve plate of high flux steel ball sample adding device；
Wherein, 1- pedestals, 2- upper sieve plates, sieve plate in 3-, sieve plate under 4-, the springs of 5- first, 6- second springs.
Specific embodiment
The present invention is described in detail with reference to the accompanying drawings and examples.
Embodiment 1：
As shown in figure 1, a kind of high flux steel ball sample adding device, including pedestal 1, upper sieve plate 2, middle sieve plate 3, lower sieve plate 4.Base
Seat 1 is the cuboid of upper and lower opening, and upper sieve plate 2 and lower sieve plate 4 are fixedly connected with the side wall of pedestal 1 respectively, the length of middle sieve plate 3
Less than the length of pedestal 1, it is respectively provided on two sides with protrusive board, and described protrusive board inserts in the perforate of base side wall, middle sieve plate 3 is consolidated
Due between upper and lower sieve plate；In order under ensureing original state, the 96 holes alignment of upper sieve plate and middle sieve plate, in the protrusive board of middle sieve plate
Side is provided with 2 rectangular v notch vs, and the first spring 5, second spring 6 are located in breach, described spring respectively with the side of pedestal 1
Wall, middle sieve plate 3 are connected, and middle sieve plate 3 can automatically reset under the elastic reaction of spring；Upper, middle and lower sieve plate is uniformly provided with 8 respectively
96 hole Heterogeneous Permutations of 96 holes of the row of row × 12,96 hole alignments of upper sieve plate and middle sieve plate, middle sieve plate and lower sieve plate, i.e., under
Space of the hole position of sieve plate between the holes of middle sieve plate, 96 holes of middle sieve plate can be made with by the protrusive board on sieve plate in promotion
The hole of sieve plate is perfectly aligned.
In order to ensure steel ball sample adding device every time to the depth that a steel ball, upper sieve plate and the hole of middle sieve plate 96 are added in centrifuge tube
Equal to the diameter of sample introduction steel ball, the aperture in 96 holes of upper, middle and lower sieve plate is equal to diameter of the steel ball to degree.
As shown in Figure 2,3, upper sieve plate 2 and lower sieve plate 4 are respectively equipped with protrusive board, and described protrusive board is inserted in the perforate of pedestal 1,
Pedestal is fixed on by upper and lower sieve plate is parallel.
The sample injection method of high flux steel ball sample adding device of the present invention, comprises the following steps that：
1st, 96 holes by the centrifuge tube of 96 orifice plates of DNA extraction elements respectively with lower sieve plate are alignd；
2nd, in an initial condition, 96 holes of upper sieve plate and middle sieve plate are perfectly aligned, 96 holes of lower sieve plate and upper, middle sieve
Plate Heterogeneous Permutation, grinding steel ball is positioned over the surface of upper sieve plate 2, the aperture in 96 holes of sieve plate is less than due to diameter of the steel ball, slightly
Micro- swaying base 1 makes steel ball roll into automatically in 96 holes of upper sieve plate, and now upper sieve plate and middle sieve plate have a steel ball；
3rd, the protrusive board of sieve plate makes 96 holes of middle sieve plate and lower sieve plate align in promoting, and now the steel ball in middle sieve plate hole is automatic
Fall into the hole of lower sieve plate, because 96 holes of lower sieve plate and 96 orifice plates of DNA extraction elements are corresponding, the steel ball of lower sieve plate can be straight
In tapping into the centrifuge tube of 96 orifice plates, can batch to being separately added into one piece of steel ball in centrifuge tube；
4th, sieve plate 3 in being unclamped after the completion of steel ball is added, middle sieve plate 3 resets under the elastic reaction of spring, and each hole
Alignd with the hole of upper sieve plate, the steel ball of upper sieve plate can be again introduced into middle sieve plate hole again, so repeat can quickly, batch it is complete
Into the sample-adding work of steel ball.
Claims (4)
1. a kind of high flux steel ball sample adding device, including pedestal, upper sieve plate, middle sieve plate, lower sieve plate, it is characterised in that：Pedestal (1)
It is the cuboid of upper and lower opening, the side wall of upper sieve plate (2) and lower sieve plate (4) respectively with pedestal (1) is fixedly connected, middle sieve plate (3)
Length of the length less than pedestal (1), middle sieve plate (3) is respectively provided on two sides with protrusive board, described protrusive board insertion pedestal (1) side wall
Perforate in, middle sieve plate (3) is fixed between upper sieve plate (2), lower sieve plate (4)；2 are provided with the protrusive board side of middle sieve plate (3)
Rectangular v notch v, the first spring (5), second spring (6) in breach, described spring side wall respectively with pedestal (1), in
Sieve plate (3) is connected；Upper sieve plate (2), middle sieve plate (3), lower sieve plate (4) be uniformly provided with 8 row × 12 row 96 holes, upper sieve plate (2) and
96 hole Heterogeneous Permutations of 96 hole alignments of middle sieve plate (3), middle sieve plate (3) and lower sieve plate (4), by sieve plate in promotion
Protrusive board makes 96 holes of middle sieve plate (3) be alignd with the hole of lower sieve plate.
2. high flux steel ball sample adding device according to claim 1, it is characterised in that upper sieve plate (2) and middle sieve plate (3)
The depth in 96 holes is equal to the diameter of steel ball, and the aperture in 96 holes of sieve plate (4) is straight equal to steel ball under upper sieve plate (2), middle sieve plate (3)
Footpath.
3. high flux steel ball sample adding device according to claim 1, it is characterised in that upper sieve plate (2) and lower sieve plate (4) point
Protrusive board is not provided with, and described protrusive board is inserted in the perforate of pedestal (1).
4. the sample injection method of the high flux steel ball sample adding device described in claim 1, it is characterised in that comprise the following steps：
A. 96 holes by the centrifuge tube of 96 orifice plates of DNA extraction elements respectively with lower sieve plate are alignd；
B., grinding steel ball is positioned over the surface of upper sieve plate (2), swaying base (1) makes steel ball roll into 96 holes of upper sieve plate automatically
It is interior；
C. the protrusive board of sieve plate (3) makes 96 holes of middle sieve plate and lower sieve plate align in promoting, and now the steel ball in middle sieve plate hole is automatic
Fall into the hole of lower sieve plate, and then fall into centrifuge tube；
D. sieve plate (3) in being unclamped after the completion of steel ball is added, middle sieve plate (3) resets under the elastic reaction of spring, and each hole
Alignd with the hole of upper sieve plate, the steel ball of upper sieve plate can be again introduced into middle sieve plate hole again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710024135.5A CN106754301B (en) | 2017-01-13 | 2017-01-13 | High-flux steel bead sample adding method and device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710024135.5A CN106754301B (en) | 2017-01-13 | 2017-01-13 | High-flux steel bead sample adding method and device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106754301A true CN106754301A (en) | 2017-05-31 |
| CN106754301B CN106754301B (en) | 2023-04-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710024135.5A Active CN106754301B (en) | 2017-01-13 | 2017-01-13 | High-flux steel bead sample adding method and device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11434481B2 (en) | 2017-07-03 | 2022-09-06 | University Of Utah Research Foundation | Rapid non-destructive genetic material collection |
| CN106754301B (en) * | 2017-01-13 | 2023-04-07 | 湖北省农业科学院粮食作物研究所 | High-flux steel bead sample adding method and device |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105296469A (en) * | 2015-11-26 | 2016-02-03 | 江苏省农业科学院 | Application of plant leaf grinding device for high-throughput rapid DNA extraction |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754301B (en) * | 2017-01-13 | 2023-04-07 | 湖北省农业科学院粮食作物研究所 | High-flux steel bead sample adding method and device |
-
2017
- 2017-01-13 CN CN201710024135.5A patent/CN106754301B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105296469A (en) * | 2015-11-26 | 2016-02-03 | 江苏省农业科学院 | Application of plant leaf grinding device for high-throughput rapid DNA extraction |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754301B (en) * | 2017-01-13 | 2023-04-07 | 湖北省农业科学院粮食作物研究所 | High-flux steel bead sample adding method and device |
| US11434481B2 (en) | 2017-07-03 | 2022-09-06 | University Of Utah Research Foundation | Rapid non-destructive genetic material collection |
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| Publication number | Publication date |
|---|---|
| CN106754301B (en) | 2023-04-07 |
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