CN106750387A - A kind of method that high temperature spray-drying prepares γ polyglutamic acid powder - Google Patents
A kind of method that high temperature spray-drying prepares γ polyglutamic acid powder Download PDFInfo
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Abstract
The invention discloses a kind of method that high temperature spray-drying prepares γ polyglutamic acid powder.Extract γ polyglutamic acid extract solutions and enter spray drying tower, 90 120 DEG C of 180 250 DEG C of drying tower EAT and leaving air temp are set, γ polyglutamic acid extract solutions are spray-dried to obtain γ polyglutamic acid powder；Described γ polyglutamic acid extract solutions, the concentration that it contains γ polyglutamic acids is mass fraction 5 20%, and pH is 38.The present invention is dried powder processed using high temperature spray-drying method to γ polyglutamic acid extract solutions, not only reduces production cost, improves industrial production efficiency, and have the advantages that energy-saving and emission-reduction, environmental protection.The present invention prepares the production technology of γ polyglutamic acid powder by high temperature spray-drying, with increasing economic efficiency and promote the realistic meaning of industry development, while profit is created for enterprise, also to bio-polymer material China be applied clinically and popularized and positive impetus is played in industrialization, improve the technology content and international competitiveness of product.
Description
Technical field
The invention belongs to technical field of drying, and in particular to a kind of gamma-polyglutamic acid produced by microbial fermentation is carried
Take the method that liquid is prepared into powder through high temperature spray-drying.
Background technology：
Gamma-polyglutamic acid is a kind of glutamic acid polymer produced by microbial fermentation, with moisturizing, maintains skin bullet
Property, lifting NMF content, the whitening colour of skin, the effects such as promote nutritional ingredient to absorb, can be carried out by microbial fermentation
Industrial production, is widely used in the industry fields such as cosmetics, food, medicine and agricultural.Gamma-polyglutamic acid be amino acid polymerization and
Into polypeptide, belong to heat-sensitive materials, easy deactivation causes its space structure to be destroyed to polypeptide at high temperature, after denaturation
Polypeptide is water insoluble, loses original effect and bioactivity.Therefore, gamma-polyglutamic acid zymotic fluid generally uses freezing after purification
Vacuum drying legal system powder, low temperature dewatering is to ensure the bioactivity such as purity and solubility of gamma-polyglutamic acid powder, but freezing
Vacuum drying electric energy consumption is huge, drying time (24-48h) long increases drying area ability preferably, it is necessary to material divides expansion
Be dried, and dry after material caking need to process grinding sieving after be only powder product.The items of vacuum freezedrying
Unfavorable factor reduces the production efficiency of the heavy industrialization of gamma-polyglutamic acid powder, and equipment investment high cost, energy consumption is big,
Output is low, is the high main cause of gamma-polyglutamic acid powder production cost.
High temperature spray-drying is that feed liquid is separated into tiny droplet using atomizer, and is steamed rapidly in heated drying medium
Hair solvent forms the dry technology of power-product.Using steam, electric Hybrid Heating, energy consumption is low, wink-dry speed for spray drying
Hurry up, the time is short, after atomization, its surface area moment increases several times to feed liquid, and the contact area with hot-air increases, inside droplet
Moisture content is greatly shortened to the path of external migration, improves heat and mass speed, and drying time is only that 5-35s or so can evaporate
Fall in material 90% -95% moisture content.Secondly, material is not subject to high temperature in itself, although the hot blast temperature of spray drying compares
Height, but when droplet is contacted, most of heat is all used for moisture evaporation, so temperature of outgoing air is not high, for some thermal sensitivity things
Material also can guarantee that its product quality.Product can be made powder or hollow ball is made after spray drying, therefore product has good dispersion
Property and dissolubility.Spray drying production process is simple, is suitable for serialization large-scale production.In sum, high temperature spray-drying
Have the advantages that disengagement area is big, drying time is short, few to active ingredient destruction, be increasingly being applied to temperature-sensitive material
Drying.
The content of the invention：
It is an object of the invention to provide one kind ensure gamma-polyglutamic acid purity, solubility and silk slide skin sense isoreactivity and
On the premise of effect, effectively reduce the energy consumption of drying powder processed, shorten drying time, form uniform after extract solution is spray-dried
Powder is settled at one go, without the method that the high temperature spray-drying of additional processing grinding screening prepares gamma-polyglutamic acid powder.
The method that high temperature spray-drying of the invention prepares gamma-polyglutamic acid powder, it is characterised in that including following step
Suddenly：
Extract gamma-polyglutamic acid extract solution and enter spray drying tower, 180-250 DEG C of drying tower EAT and air-out are set
90-120 DEG C of temperature, gamma-polyglutamic acid extract solution is spray-dried to obtain gamma-polyglutamic acid powder；
Described gamma-polyglutamic acid extract solution, the concentration that it contains gamma-polyglutamic acid is 3- for mass fraction 5-20%, pH
8。
The gamma-polyglutamic acid extract solution is bacillus subtilis (Bacillus subtilis) PGA-7 fermenting and producings
Gamma-polyglutamic acid zymotic fluid.Preferably according to the patent No.：ZL 200610122640.5, it is entitled：Gamma-polyglutamic acid is produced
Method disclosed in raw bacterium and the method using the preparing gamma-polyglutamic acid prepares the fermentation containing gamma-polyglutamic acid
Liquid, then again through it is degerming, decolourize, essence take and concentrate etc. step after extract solution, γ in control gamma-polyglutamic acid extract solution-gather
Aminoglutaric acid concentration is 3-8 for mass fraction 5-20%, pH.
The gamma-polyglutamic acid is the salt of gamma-polyglutamic acid or gamma-polyglutamic acid.The salt of gamma-polyglutamic acid be selected from γ-
Polyglutamic acid sodium, gamma-polyglutamic acid potassium, gamma-polyglutamic acid calcium, gamma-polyglutamic acid magnesium, gamma-polyglutamic acid zinc and γ-polyglutamic
Sour iron.
Described atomizer is spray-dried using compression sprayer, centrifugal sprayer or air-flowing type sprayer.
Patent of the present invention is dried powder processed using high temperature spray-drying method to gamma-polyglutamic acid extract solution, with excellent
Technological parameter is set, on the premise of ensureing that gamma-polyglutamic acid purity, solubility and silk slide skin sense isoreactivity and effect, effectively
Reduce the energy consumption of drying powder processed, shorten drying time, forming uniform powder after extract solution is spray-dried settles at one go, without
Additional processing grinding screening.
Patent of the present invention is dried powder processed using high temperature spray-drying method to gamma-polyglutamic acid extract solution, not only reduces
Production cost, improves industrial production efficiency, and have the advantages that energy-saving and emission-reduction, environmental protection.The present invention is sprayed by high temperature
Mist drying prepares the production technology of gamma-polyglutamic acid powder, with increasing economic efficiency and promote the realistic meaning of industry development,
For enterprise create profit while, also to bio-polymer material China be applied clinically and popularized and industrialization plays positive
Impetus, improves the technology content and international competitiveness of product.
Technique effect of the invention is embodied in：
1st, the gamma-polyglutamic acid extract solution after present invention use is purified, spray effect is disturbed without other impurities.With this hair
Bright technological parameter：Under conditions of extract solution mass concentration 5-20% and pH 3-8, what gamma-polyglutamic acid will not be spray dried
High temperature space structure, will not deactivation into insoluble matter, it still keeps the bioactivity such as good purity and solubility.By
There is high polymerization degree and high viscosity in gamma-polyglutamic acid, the more excellent microsphere powder of proterties is formed after its spray drying；
2nd, the present invention uses high temperature spray-drying, and EAT is 180-250 DEG C, and leaving air temp is 90-120 DEG C, this temperature
Degree sets higher than general drying process with atomizing temperature, on the premise of gamma-polyglutamic acid bioactivity is not destroyed, greatly improves
Dry feed speed (50-100L/h), powder moisture is low after drying, finer and smoother uniform；
3rd, the gamma-polyglutamic acid powder-product using obtained by high temperature spray-drying method of the invention, gamma-polyglutamic acid purity >=
90%, white uniformly dries fine powder to faint yellow, can be completely soluble, and water-soluble rear skin sense silk is sliding soft, and its biology is fully retained
Active effect, can very well be applied to cosmetic industry field；
4th, to prepare gamma-polyglutamic acid powder using high temperature spray-drying method efficiently convenient for the present invention, greatly reduces production
Cost and shortening production time, technique more environmental protection promote industry healthy development, improve gamma-polyglutamic acid product
Competitiveness.
Specific embodiment
Following examples are further illustrated to of the invention, rather than limitation of the present invention.
The fermented bacterium bacillus subtilis used in following examples is bacillus subtilis (Bacillus
Subtilis) PGA-7, it is preserved in China typical culture collection center, and its deposit number is：CCTCC NO：M206102,
The bacterium is disclosed in the patent No.：ZL 200610122640.5, it is entitled：Gamma-polyglutamic acid generating bacterium and utilize the bacterial strain system
In the patent of the method for standby gamma-polyglutamic acid.
Embodiment 1：
1st, gamma-polyglutamic acid zymotic fluid is according to the patent No.：ZL 200610122640.5, it is entitled：γ-poly- paddy
Method disclosed in propylhomoserin producing strains and the method using the preparing gamma-polyglutamic acid prepare containing γ-polyglutamic
The zymotic fluid of acid.
The preparation method of the zymotic fluid containing gamma-polyglutamic acid of the present embodiment is：By bacillus subtilis (Bacillus
Subtilis) PGA-7 is inoculated into the fermentation medium of fermentation tank, and 37 DEG C of constant temperature stir cultures are after 60 hours, harvest must contain γ-
The zymotic fluid of polyglutamic acid, the composition of described fermentation medium is：Every liter contains glucose 60g, NH4NO3 20g、K2HPO4
1g、MgSO4·7H2O 0.01g、FeCl3·6H2O 0.05g、CaCl2·2H2O 0.2g、MnSO4·H2It is O 0.104g, balance of
Water.Its compound method is according to mentioned component and content, by glucose 60g, NH4NO3 20g、K2HPO4 1g、MgSO4·
7H2O0.01g、FeCl3·6H2O 0.05g、CaCl2·2H2O 0.2g、MnSO4·H2O 0.104g are added to the water, Ran Houyong
Water constant volume adjusts pH value (before sterilizing) to be sterilized 30 minutes for 7.0,121 DEG C to 1L with NaOH and HCl.
2nd, the preparation of extract solution：Plate-frame filtering is carried out to the zymotic fluid containing gamma-polyglutamic acid degerming, circulated 3-5 times, added
3% activated carbon, while 40-80 DEG C of heating and thermal insulation, plate-frame filtering decolouring, prepared gamma-polyglutamic acid is extracted after essence takes concentration volume
Liquid, concentration of the gamma-polyglutamic acid extract solution containing gamma-polyglutamic acid is mass fraction 5%, and pH is 3.
It is mass fraction 5% by the concentration of gamma-polyglutamic acid, pH is that 3.0 gamma-polyglutamic acid extract solution carries out high temperature spray
Mist is dried, and sets 90 DEG C of 180 DEG C of EAT and leaving air temp, and gamma-polyglutamic acid extract solution is sprayed into superfine by atomizer
Misty liquid droplets form powdery solid by instantaneous high-temperature rapid vaporization in tower simultaneously, powdery solid receives through 2 grades of cyclone separators
Gamma-polyglutamic acid powder is obtained after collection.Gamma-polyglutamic acid powder is white uniformity fine powder, through UV spectrophotometer measuring meter
It is 90.6% to calculate purity, and powder is completely soluble into colourless transparent solution, and aqueous solution skin sense silk is sliding soft, and proterties is good.
Embodiment 2：
The preparation process of the zymotic fluid containing gamma-polyglutamic acid is same as Example 1.
To contain again the zymotic fluid of gamma-polyglutamic acid through it is degerming, decolourize, essence takes and obtains after concentrate gamma-polyglutamic acid extraction
Liquid, the concentration that it contains gamma-polyglutamic acid is mass fraction 15%, and pH is 3.0.This gamma-polyglutamic acid extract solution is carried out into high temperature
Spray drying, sets 110 DEG C of 220 DEG C of EAT and leaving air temp, and gamma-polyglutamic acid extract solution sprays poling by atomizer
Thin misty liquid droplets form powdery solid by instantaneous high-temperature rapid vaporization in tower simultaneously, and powdery solid is through 2 grades of cyclone separators
Gamma-polyglutamic acid powder is obtained after collection.Gamma-polyglutamic acid powder is faint yellow uniform fine powder, is examined through ultraviolet specrophotometer
It is 91% to survey calculated purity, and powder is completely soluble into colourless to light yellow transparent solution, and aqueous solution skin sense silk is sliding soft, proterties
Well.
Embodiment 3：
The preparation process of the zymotic fluid containing gamma-polyglutamic acid is same as Example 1.
To contain again the zymotic fluid of gamma-polyglutamic acid through it is degerming, decolourize, essence takes and obtains after concentrate gamma-polyglutamic acid extraction
Liquid, the concentration that it contains gamma-polyglutamic acid is mass fraction 8%, and pH is 7.0.This gamma-polyglutamic acid extract solution is carried out into high temperature spray
Mist is dried, and sets 90 DEG C of 200 DEG C of EAT and leaving air temp, and gamma-polyglutamic acid extract solution is sprayed into superfine by atomizer
Misty liquid droplets form powdery solid by instantaneous high-temperature rapid vaporization in tower simultaneously, powdery solid receives through 2 grades of cyclone separators
Gamma-polyglutamic acid powder is obtained after collection.Gamma-polyglutamic acid powder is faint yellow uniform fine powder, through UV spectrophotometer measuring
Calculated purity is 95.1%, and powder is completely soluble into colourless transparent solution, and aqueous solution skin sense silk is sliding soft, and proterties is good.
Embodiment 4：
The preparation process of the zymotic fluid containing gamma-polyglutamic acid is same as Example 1.
To contain again the zymotic fluid of gamma-polyglutamic acid through it is degerming, decolourize, essence takes and obtains after concentrate gamma-polyglutamic acid extraction
Liquid, the concentration that it contains gamma-polyglutamic acid is mass fraction 20%, and pH is 5.5.This gamma-polyglutamic acid extract solution is carried out into high temperature
Spray drying, sets 120 DEG C of 200 DEG C of EAT and leaving air temp, and gamma-polyglutamic acid extract solution sprays poling by atomizer
Thin misty liquid droplets form powdery solid by instantaneous high-temperature rapid vaporization in tower simultaneously, and powdery solid is through 2 grades of cyclone separators
Gamma-polyglutamic acid powder is obtained after collection.Gamma-polyglutamic acid powder is faint yellow uniform fine powder, is examined through ultraviolet specrophotometer
It is 94.4% to survey calculated purity, and powder is completely soluble into colourless to light yellow transparent solution, and aqueous solution skin sense silk is sliding soft, property
Shape is good.
Embodiment 5：
1st, gamma-polyglutamic acid zymotic fluid is according to the patent No.：ZL 200610122640.5, it is entitled：γ-poly- paddy
Method disclosed in propylhomoserin producing strains and the method using the preparing gamma-polyglutamic acid is prepared containing gamma-polyglutamic acid
Zymotic fluid.
The preparation method of the zymotic fluid containing gamma-polyglutamic acid of the present embodiment is：By bacillus subtilis (Bacillus
Subtilis) PGA-7 is inoculated into the fermentation medium of fermentation tank, and 37 DEG C of constant temperature stir cultures are after 90 hours, harvest must contain γ-
The zymotic fluid of polyglutamic acid, the composition of described fermentation medium is：Every liter contains sucrose 90g, (NH4)2SO4 20g、K2HPO4
1g、MgSO4·7H2O 0.06g、FeCl3·6H2O 0.01g、CaCl2·2H2O 0.15g、MnSO4·H2It is O 0.05g, balance of
Water.Its compound method is according to mentioned component and content, by sucrose 90g, (NH4)2SO4 20g、K2HPO4 1g、MgSO4·7H2O
0.06g、FeCl3·6H2O 0.01g、CaCl2·2H2O 0.15g、MnSO4·H2O 0.05g are added to the water, then fixed with water
Hold 1L, and adjust Medium's PH Value (before sterilizing) to be 7.0,121 DEG C with NaOH and HCl and sterilize 30 minutes.
To contain again the zymotic fluid of gamma-polyglutamic acid through it is degerming, decolourize, essence takes and obtains after concentrate gamma-polyglutamic acid extraction
Liquid, the concentration that it contains gamma-polyglutamic acid is mass fraction 12%, and pH is 6.0.This gamma-polyglutamic acid extract solution is carried out into high temperature
Spray drying, sets 90 DEG C of 250 DEG C of EAT and leaving air temp, and gamma-polyglutamic acid extract solution sprays poling by atomizer
Thin misty liquid droplets form powdery solid by instantaneous high-temperature rapid vaporization in tower simultaneously, and powdery solid is through 1 grade of cyclone separator
Gamma-polyglutamic acid powder is obtained after collection.Gamma-polyglutamic acid powder is faint yellow uniform fine powder, is examined through ultraviolet specrophotometer
It is 92.0% to survey calculated purity, and powder is completely soluble into colourless to light yellow transparent solution, and aqueous solution skin sense silk is sliding soft, property
Shape is good.
Embodiment 6：
The preparation process of the zymotic fluid containing gamma-polyglutamic acid is same as Example 1.
To contain again the zymotic fluid of gamma-polyglutamic acid through it is degerming, decolourize, essence takes and obtains after concentrate gamma-polyglutamic acid extraction
Liquid, the concentration that it contains gamma-polyglutamic acid is mass fraction 12%, and pH is 6.0.This gamma-polyglutamic acid extract solution is carried out into high temperature
Spray drying, sets 100 DEG C of 180 DEG C of EAT and leaving air temp, and gamma-polyglutamic acid extract solution sprays poling by atomizer
Thin misty liquid droplets form powdery solid by instantaneous high-temperature rapid vaporization in tower simultaneously, and powdery solid is through 2 grades of cyclone separators
Gamma-polyglutamic acid powder is obtained after collection.Gamma-polyglutamic acid powder is faint yellow uniform fine powder, is examined through ultraviolet specrophotometer
It is 97.5% to survey calculated purity, and powder is completely soluble into colourless transparent solution, and aqueous solution skin sense silk is sliding soft, and proterties is good.
Embodiment 7：
The preparation process of the zymotic fluid containing gamma-polyglutamic acid is same as Example 1.
To contain again the zymotic fluid of gamma-polyglutamic acid through it is degerming, decolourize, essence takes and obtains after concentrate gamma-polyglutamic acid extraction
Liquid, the concentration that it contains gamma-polyglutamic acid is mass fraction 18%, and pH is 8.0.This gamma-polyglutamic acid extract solution is carried out into high temperature
Spray drying, sets 110 DEG C of 210 DEG C of EAT and leaving air temp, and gamma-polyglutamic acid extract solution sprays poling by atomizer
Thin misty liquid droplets form powdery solid by instantaneous high-temperature rapid vaporization in tower simultaneously, and powdery solid is through 2 grades of cyclone separators
Gamma-polyglutamic acid powder is obtained after collection.Gamma-polyglutamic acid powder is faint yellow uniform fine powder, is examined through ultraviolet specrophotometer
It is 90.2% to survey calculated purity, and powder is completely soluble into colourless transparent solution, and aqueous solution skin sense silk is sliding soft, and proterties is good.
Embodiment 8：
The preparation process of the zymotic fluid containing gamma-polyglutamic acid is same as Example 1.
To contain again the zymotic fluid of gamma-polyglutamic acid through it is degerming, decolourize, essence takes and obtains after concentrate gamma-polyglutamic acid extraction
Liquid, the concentration that it contains gamma-polyglutamic acid is mass fraction 15%, and pH is 4.0.This gamma-polyglutamic acid extract solution is carried out into high temperature
Spray drying, sets 90 DEG C of 190 DEG C of EAT and leaving air temp, and gamma-polyglutamic acid extract solution sprays poling by atomizer
Thin misty liquid droplets form powdery solid by instantaneous high-temperature rapid vaporization in tower simultaneously, and powdery solid is through 2 grades of cyclone separators
Gamma-polyglutamic acid powder is obtained after collection.Gamma-polyglutamic acid powder is faint yellow uniform fine powder, is examined through ultraviolet specrophotometer
It is 93.4% to survey calculated purity, and powder is completely soluble into colourless to light yellow transparent solution, and aqueous solution skin sense silk is sliding soft, property
Shape is good.
Comparative example 1：
The preparation process of the broth extraction liquid containing gamma-polyglutamic acid is same as Example 1.
The zymotic fluid that to contain gamma-polyglutamic acid again is degerming, decolourize, essence takes and obtains gamma-polyglutamic acid extract solution after concentrating,
The concentration that it contains gamma-polyglutamic acid is mass fraction 4%, and pH is 4.0.This gamma-polyglutamic acid extract solution is carried out into high-temperature spray
Dry, 90 DEG C of 190 DEG C of EAT and leaving air temp are set, gamma-polyglutamic acid extract solution is sprayed into superfine by atomizer
Misty liquid droplets form powdery solid by instantaneous high-temperature rapid vaporization in tower simultaneously, and powdery solid is through 2 grades of cyclone collections
After obtain gamma-polyglutamic acid powder.This gamma-polyglutamic acid powder space structure by high temperature, deactivation turns into insoluble
Thing (water insoluble).
Comparative example 2：
The preparation process of the broth extraction liquid containing gamma-polyglutamic acid is same as Example 1.
The zymotic fluid that to contain gamma-polyglutamic acid again is degerming, decolourize, essence takes and obtains gamma-polyglutamic acid extract solution after concentrating,
The concentration that it contains gamma-polyglutamic acid is mass fraction 21%, and pH is 4.0.This gamma-polyglutamic acid extract solution is carried out into high-temperature spray
Dry, 90 DEG C of 190 DEG C of EAT and leaving air temp are set, gamma-polyglutamic acid extract solution is sprayed into superfine by atomizer
Misty liquid droplets form powdery solid by instantaneous high-temperature rapid vaporization in tower simultaneously, and powdery solid is through 2 grades of cyclone collections
After obtain gamma-polyglutamic acid powder.This gamma-polyglutamic acid powder space structure by high temperature, deactivation turns into insoluble
Thing (water insoluble).
Comparative example 3：
The preparation process of the broth extraction liquid containing gamma-polyglutamic acid is same as Example 1.
The zymotic fluid that to contain gamma-polyglutamic acid again is degerming, decolourize, essence takes and obtains gamma-polyglutamic acid extract solution after concentrating,
The concentration that it contains gamma-polyglutamic acid is mass fraction 10%, and pH is 2.0.This gamma-polyglutamic acid extract solution is carried out into high-temperature spray
Dry, 90 DEG C of 190 DEG C of EAT and leaving air temp are set, gamma-polyglutamic acid extract solution is sprayed into superfine by atomizer
Misty liquid droplets form powdery solid by instantaneous high-temperature rapid vaporization in tower simultaneously, and powdery solid is through 2 grades of cyclone collections
After obtain gamma-polyglutamic acid powder.This gamma-polyglutamic acid powder space structure by high temperature, deactivation turns into insoluble
Thing (water insoluble).
Comparative example 4：
The preparation process of the broth extraction liquid containing gamma-polyglutamic acid is same as Example 1.
The zymotic fluid that to contain gamma-polyglutamic acid again is degerming, decolourize, essence takes and obtains gamma-polyglutamic acid extract solution after concentrating,
The concentration that it contains gamma-polyglutamic acid is mass fraction 10%, and pH is 9.0.This gamma-polyglutamic acid extract solution is carried out into high-temperature spray
Dry, 90 DEG C of 190 DEG C of EAT and leaving air temp are set, gamma-polyglutamic acid extract solution is sprayed into superfine by atomizer
Misty liquid droplets form powdery solid by instantaneous high-temperature rapid vaporization in tower simultaneously, and powdery solid is through 2 grades of cyclone collections
After obtain gamma-polyglutamic acid powder.This gamma-polyglutamic acid powder space structure by high temperature, deactivation turns into insoluble
Thing (water insoluble).
Claims (5)
1. a kind of method that high temperature spray-drying prepares gamma-polyglutamic acid powder, it is characterised in that comprise the following steps：
Extract gamma-polyglutamic acid extract solution and enter spray drying tower, 180-250 DEG C of drying tower EAT and leaving air temp are set
90-120 DEG C, gamma-polyglutamic acid extract solution is spray-dried to obtain gamma-polyglutamic acid powder；
Described gamma-polyglutamic acid extract solution, the concentration that it contains gamma-polyglutamic acid is 3-8 for mass fraction 5-20%, pH.
2. method according to claim 1, it is characterised in that the gamma-polyglutamic acid extract solution is bacillus subtilis
The gamma-polyglutamic acid zymotic fluid of (Bacillus subtilis) PGA-7 fermenting and producings.
3. method according to claim 2, it is characterised in that the gamma-polyglutamic acid extract solution is according to the patent No.：
ZL200610122640.5, it is entitled：Gamma-polyglutamic acid generating bacterium and the side using the preparing gamma-polyglutamic acid
Method disclosed in method prepares the zymotic fluid containing gamma-polyglutamic acid, after then taking and concentrate through degerming, decolouring, essence again
Extract solution, gamma-polyglutamic acid concentration is for mass fraction 5-20%, pH are 3-8 in controlling gamma-polyglutamic acid extract solution.
4. method according to claim 1, it is characterised in that the gamma-polyglutamic acid is gamma-polyglutamic acid or γ-poly-
The salt of glutamic acid.
5. method according to claim 1, it is characterised in that described gamma-polyglutamic acid extract solution is spray-dried
To gamma-polyglutamic acid powder it is spray-dried using compression sprayer, centrifugal sprayer or air-flowing type sprayer.
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| CN110354515A (en) * | 2019-06-05 | 2019-10-22 | 河南慧宝源生物医药科技有限公司 | A kind of drying means of gamma-polyglutamic acid |
| CN114213179A (en) * | 2021-12-20 | 2022-03-22 | 辽宁中科生物工程股份有限公司 | Organic polymeric magnesium agent and preparation method thereof |
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| CN114213179A (en) * | 2021-12-20 | 2022-03-22 | 辽宁中科生物工程股份有限公司 | Organic polymeric magnesium agent and preparation method thereof |
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