CN103789359A - Method for producing malic acid by using fermentation of wheat bran hydrolysate - Google Patents
Method for producing malic acid by using fermentation of wheat bran hydrolysate Download PDFInfo
- Publication number
- CN103789359A CN103789359A CN201410036126.4A CN201410036126A CN103789359A CN 103789359 A CN103789359 A CN 103789359A CN 201410036126 A CN201410036126 A CN 201410036126A CN 103789359 A CN103789359 A CN 103789359A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- testa tritici
- aspergillus
- acid
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention provides a method for producing malic acid by using fermentation of wheat bran hydrolysate. The method comprises the following steps: preparing wheat bran hydrolysate by an acid hydrolysis method, and then carrying out detoxification treatment on a fermentation inhibitor contained in the hydrolysis liquid glucose by using activated carbon; and selecting aspergillus parasiticus CICC40365 with good adaptability, and matching with the optimized fermentation nutrients to mix and ferment, so as to obtain malic acid fermentation liquor. The method has advantages of being high in wheat bran hydrolysis rate, fast in reaction speed, simple in process and low in cost, the hydrolysis liquid glucose is low in loss in the fermentation process by good detoxification treatment, and the adopted aspergillus has good adaptability, and is high in conversion rate of the malic acid. In addition, the method is free of poison and harm in the production process, environment-friendly and energy-saving, applicable to industrial large-scale production, and significant in economic benefits may be achieved, energy crisis in China and in the world can be effectively relieved.
Description
Technical field
The invention belongs to fermentation technical field, relate in particular to a kind of method of utilizing the fermentation of Testa Tritici hydrolysis sugar to produce oxysuccinic acid.
Background technology
L MALIC ACID (L-malicacid), has another name called hydroxy-butanedioic acid, is a kind of saturated diprotic acid.Outward appearance is white crystals or crystalline powder, has stronger water absorbability, and soluble in water, ethanol has special tart flavour, is extensively present in a kind of natural organic acids in vegetables and fruit, and its content is only second to citric acid, and has special local flavor.In addition, L MALIC ACID is also the important intermediate in body metabolism, is easily absorbed by the body.Therefore, be widely used in food, makeup, medicine and other fields as good acidic flavoring agent and preservation agent.
Wheat is one of staple food crop of China, and its annual production has exceeded more than 100,000,000 ton.Testa Tritici is the Main By product in wheat flour milling process, annual output nearly wheat processing amount 20%, this is a very large resource.In Testa Tritici, be rich in polysaccharide, protein, fat, VITAMIN, mineral matter and other components.But, domestic substantially by Testa Tritici for fodder industry, utilization ratio is very low, rich cellulose and hemicellulose in Testa Tritici, adopt suitable hydrolysis process, be translated into monose and then the fermentation that can utilize for microorganism and prepare organic acid, this high-efficiency comprehensive utilization to wheat bran and improve its added value and have great importance.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing the fermentation of Testa Tritici hydrolysis sugar to produce oxysuccinic acid, be intended to solve the low problem of existing Testa Tritici utilization ratio.
The present invention is achieved in that 1, a kind of method of utilizing the fermentation of Testa Tritici hydrolysis sugar to produce oxysuccinic acid, comprises the following steps:
(1) sulphuric acid soln that is 2%～4% by Testa Tritici powder and mass concentration is 1g:(7.5～12.5 by mass volume ratio) ml mixes, and at 121 ℃, reacts 1h, cooling, filter, and gets filtrate; In filtrate, add excess amount of Ca (OH) 2 to regulate pH to 10～11, under 8000r/min condition, centrifugal 10min, gets supernatant liquor, in described supernatant liquor, add acid solution adjusting pH to neutral, under 8000r/min condition, centrifugal 10min, gets supernatant liquor, obtains Testa Tritici hydrolysis sugar liquid;
(2) toward the gac that is incorporated as its quality 10% in described Testa Tritici hydrolysis sugar liquid, detoxification 1h under 40 ℃ of bath temperatures, 120r/min hunting speed, by reacting liquid filtering, gets filtrate, obtaining the liquid glucose that ferments, is 100g/L by described fermentation liquid glucose evaporation concentration to total sugar concentration;
(3), after fermentation liquid glucose step (2) being obtained mixes with nutritive substance, sterilizing 20min at 121 ℃ of temperature, obtains fermentation culture; Wherein, after described fermentation liquid glucose mixes with nutritive substance, cumulative volume calculates by 1L, and the quality of described fermentation liquid glucose and each nutritive substance is respectively: fermentation liquid glucose 100g～110g, ammonium sulfate 1.9g～2.2g, yeast soak powder 2.7g～3.3g, potassium primary phosphate 0.1g～0.15g, dipotassium hydrogen phosphate 0.2g～0.3g, magnesium sulfate 0.1g～0.2g, manganous sulfate 0.1g～0.2g, ferrous sulfate 0.05g～0.07g, calcium carbonate 70g～100g;
(4) add (1～10) × 10 toward the fermentation culture in step (3)
4cFU/mL aspergillus tubigensis seed culture fluid, 32 ℃, air air flow 2.5～30L/min, stirring velocity 160r/min condition bottom fermentation 5 days, collects fermented liquid, obtains the oxysuccinic acid existing with the form of calcium salt; Wherein, the volume ratio of described fermentation culture and aspergillus tubigensis seed culture fluid is 1:10.
Preferably, in step (1), described Testa Tritici powder is prepared by following steps: Testa Tritici is dried at 80 ℃ of temperature, the Testa Tritici after drying is pulverized, crossed 60 mesh sieves, obtain Testa Tritici powder.
Preferably, in step (1), described interpolation Ca (OH) 2 is pressed powder, analytical pure, purity >=95%; Described acid solution is the sulfuric acid of mass concentration 3%.
Preferably, in step (4), described aspergillus tubigensis seed culture liquid and preparation method thereof is as follows: it is in 10:1～15:1 seed culture medium that the aspergillus spore liquid that is 1 × 106CFU/mL by concentration is linked into its volume ratio, under 30 ℃～32 ℃, 180r/min～200r/min condition, cultivates 16～18h; Wherein, described seed culture medium calculates and comprises following composition by 1L: glucose 30g, ammonium sulfate 1.9g～2.2g, yeast soak powder 2.7g～3.3g, potassium primary phosphate 0.1g～0.15g, dipotassium hydrogen phosphate 0.2g～0.3g, magnesium sulfate 0.1g～0.2g, manganous sulfate 0.1g～0.2g, ferrous sulfate 0.05g～0.07g.
Preferably, described aspergillus bacterial classification is aspergillus (Aspergillusparasiticus) CICC40365, is purchased from Chinese industrial microbial strains preservation center.
Compare and the shortcoming and defect of prior art, the present invention has following beneficial effect:
(1) the present invention adopts the method for acidolysis to prepare Testa Tritici hydrolysis sugar, and the method has the features such as percent hydrolysis is high, speed of response is fast, technique is simple, cost is low.
(2) the present invention has carried out gac detoxification treatment to hydrolyzed solution, and the fermentation inhibitory such as furfural, the acetic acid agent content in liquid glucose that obtains obviously reduces, the fine adaptation of aspergillus (Aspergillusparasiticus) CICC40365 that can be adopted in the present invention; And the sugar that the method causes loss is also lower, makes the transformation efficiency of final wheat bran higher.Macroporous resin also has better detoxification characteristic, but its material itself has toxicity, only has by ethanol and NaOH solution and repeatedly cleans and just can avoid toxicant residual, and operation is loaded down with trivial details, and itself cost is also very high.The macroporous resin detoxification of comparing, gac does not have secondary pollution problem, and cheap.
(3) bacterial classification adopting in the present invention can utilize five simultaneously, hexose, compares other bacterial strains, utilizes the efficiency of Testa Tritici hydrolyzed solution higher.
(4) the present invention utilizes agricultural and sideline product Testa Tritici to prepare L MALIC ACID for raw material, can break away from the depending on unduly of petroleum base raw material, further alleviates the energy dilemma in China and the world.And opened up a green, sustainable, potential production ways, production cost is low, has good economy.
(5) the present invention utilizes the method for microorganism fermentation to prepare oxysuccinic acid.Its advantage is in production process nontoxic, and environmental protection and energy saving are applicable to commercial scale production; The main L MALIC ACID that produces, is conducive to product separation and purification.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Embodiment 1
The preparation of A, Testa Tritici hydrolyzed solution
By Testa Tritici removal of impurities, be placed in hot air drier, dry a few hours in 80 ℃.Testa Tritici after oven dry is pulverized with plant pulverizer, crosses 60 mesh sieves, and Testa Tritici powder is loaded in sealing bag for subsequent use.
Get Testa Tritici powder 200g, adding mass concentration is 3% dilution heat of sulfuric acid 2.0L, and stirring and evenly mixing reacts 1h at 121 ℃, cooling, filter, and gets filtrate, in filtrate, adds excess amount of Ca (OH)
2adjust pH to 10～11, under 8000r/min condition, centrifugal 10min, gets supernatant liquor, is that 3% dilute sulphuric acid adjusts pH to neutral to adding mass concentration in supernatant liquor, and under 8000r/min condition, centrifugal 10min, gets supernatant liquor, obtains Testa Tritici hydrolysis sugar.
B, hydrolyzed solution detoxification treatment
200g gac is added in the Testa Tritici hydrolyzed solution that steps A obtains, is placed in shaking bath detoxification 1h under 40 ℃, 120r/min hunting speed condition, after reaction finishes, filter and to obtain fermentation liquid glucose; Be 100g/L by this fermentation liquid glucose by Rotary Evaporators evaporation concentration to total sugar concentration.
C, substratum preparation
After adding nutritive substance in fermentation liquid glucose after concentrated in step B, obtain fermention medium, pressing 1L calculates, the each concentration of component of fermention medium is: wheat bran hydrolyzed solution sugar 100g/L, and ammonium sulfate 2g/L, yeast soaks powder 3g/L, potassium primary phosphate 0.1g/L, dipotassium hydrogen phosphate 0.2g/L, magnesium sulfate 0.1g/L, manganous sulfate 0.1g/L, ferrous sulfate 0.05g/L, calcium carbonate 70g/L.
5L fermention medium is pumped in the fermentor tank of 7L scale to direct 121 ℃ of sterilizing 20min in place.
D, the preparation of described aspergillus tubigensis seed culture fluid
Method is as follows: it is in 10:1～15:1 seed culture medium that the aspergillus spore liquid that is 1 × 106CFU/mL by concentration is linked into its volume ratio, under 30 ℃～32 ℃, 180r/min～200r/min condition, cultivates 16～18h, obtains concentration for (1～10) × 10
4the aspergillus tubigensis seed culture fluid of CFU/mL; Wherein, described seed culture medium calculates and comprises following composition by 1L: glucose 30g, ammonium sulfate 1.9g～2.2g, yeast soak powder 2.7g～3.3g, potassium primary phosphate 0.1g～0.15g, dipotassium hydrogen phosphate 0.2g～0.3g, magnesium sulfate 0.1g～0.2g, manganous sulfate 0.1g～0.2g, ferrous sulfate 0.05g～0.07g.
Wherein, fermented bacterium used is aspergillus (Aspergillusparasiticus) CICC40365, is purchased from Chinese industrial microbial strains preservation center.
Oxysuccinic acid is produced in E, fermentation
By in 250mL aspergillus tubigensis seed culture fluid access fermentor tank, 32 ℃ of temperature, air air flow 2.5L/min, mixing speed 160r/min condition bottom fermentation 5 days, in gained fermented liquid, product oxysuccinic acid exists with the form of calcium salt, malic acid concentration reaches 51.38g/L, prepares 256.90g oxysuccinic acid in 5L fermented liquid.
Embodiment 2
The preparation of A, Testa Tritici hydrolyzed solution
By Testa Tritici removal of impurities, be placed in hot air drier, dry a few hours in 80 ℃.Testa Tritici after oven dry is pulverized with plant pulverizer, crosses 60 mesh sieves, and Testa Tritici powder is loaded in sealing bag for subsequent use.
Get Testa Tritici powder 15kg, adding mass concentration is 3% dilution heat of sulfuric acid 150L, and stirring and evenly mixing reacts 1h at 121 ℃, cooling, filter, and gets filtrate, in filtrate, adds excess amount of Ca (OH)
2adjust pH to 10～11, under 8000r/min condition, centrifugal 10min, gets supernatant liquor, is that 3% dilute sulphuric acid adjusts pH to neutral to adding mass concentration in supernatant liquor, and under 8000r/min condition, centrifugal 10min, gets supernatant liquor, obtains Testa Tritici hydrolysis sugar.
B, hydrolyzed solution detoxification treatment
15kg gac is added in the Testa Tritici hydrolyzed solution that steps A obtains, is placed in shaking bath detoxification 1h under 40 ℃, 120r/min hunting speed condition, after reaction finishes, filter and to obtain fermentation liquid glucose; Be 100g/L by this fermentation liquid glucose by Rotary Evaporators evaporation concentration to total sugar concentration.
C, substratum preparation
After adding nutritive substance in fermentation liquid glucose after concentrated in step B, obtain fermention medium, pressing 1L calculates, the each concentration of component of fermention medium is: wheat bran hydrolyzed solution sugar 100g/L, and ammonium sulfate 2g/L, yeast soaks powder 3g/L, potassium primary phosphate 0.1g/L, dipotassium hydrogen phosphate 0.2g/L, magnesium sulfate 0.1g/L, manganous sulfate 0.1g/L, ferrous sulfate 0.05g/L, calcium carbonate 70g/L.
450L fermention medium is pumped in the fermentor tank of 500L scale to direct 121 ℃ of sterilizing 20min in place.
D, the preparation of described aspergillus tubigensis seed culture fluid
Method is as follows: it is in 10:1～15:1 seed culture medium that the aspergillus spore liquid that is 1 × 106CFU/mL by concentration is linked into its volume ratio, under 30 ℃～32 ℃, 180r/min～200r/min condition, cultivates 16～18h, obtains concentration for (1～10) × 10
4the aspergillus tubigensis seed culture fluid of CFU/mL; Wherein, described seed culture medium calculates and comprises following composition by 1L: glucose 30g, ammonium sulfate 1.9g～2.2g, yeast soak powder 2.7g～3.3g, potassium primary phosphate 0.1g～0.15g, dipotassium hydrogen phosphate 0.2g～0.3g, magnesium sulfate 0.1g～0.2g, manganous sulfate 0.1g～0.2g, ferrous sulfate 0.05g～0.07g.
Wherein, fermented bacterium used is aspergillus (Aspergillusparasiticus) CICC40365, is purchased from Chinese industrial microbial strains preservation center.
Oxysuccinic acid is produced in E, fermentation
By in 22.5L aspergillus tubigensis seed culture fluid access fermentor tank, 32 ℃ of temperature, air air flow 30L/min, mixing speed 160r/min condition bottom fermentation 5 days, in gained fermented liquid, product oxysuccinic acid exists with the form of calcium salt, malic acid concentration reaches 49.22g/L, prepares 22.15kg oxysuccinic acid in 450L fermented liquid.
Embodiment 3
The preparation of A, Testa Tritici hydrolyzed solution
By Testa Tritici removal of impurities, be placed in hot air drier, dry a few hours in 80 ℃.Testa Tritici after oven dry is pulverized with plant pulverizer, crosses 60 mesh sieves, and Testa Tritici powder is loaded in sealing bag for subsequent use.
Get Testa Tritici powder 100g, adding mass concentration is 3% dilution heat of sulfuric acid 1.0L, and stirring and evenly mixing reacts 1h at 121 ℃, cooling, filter, and gets filtrate, in filtrate, adds excess amount of Ca (OH)
2adjust pH to 10～11, under 8000r/min condition, centrifugal 10min, gets supernatant liquor, is that 3% dilute sulphuric acid adjusts pH to neutral to adding mass concentration in supernatant liquor, and under 8000r/min condition, centrifugal 10min, gets supernatant liquor, obtains Testa Tritici hydrolysis sugar.
B, hydrolyzed solution detoxification treatment
100g gac is added in the Testa Tritici hydrolyzed solution that steps A obtains, is placed in shaking bath detoxification 1h under 40 ℃, 120r/min hunting speed condition, after reaction finishes, filter and to obtain fermentation liquid glucose; Be 100g/L by this fermentation liquid glucose by Rotary Evaporators evaporation concentration to total sugar concentration.
C, substratum preparation
After adding nutritive substance in fermentation liquid glucose after concentrated in step B, obtain fermention medium, pressing 1L calculates, the each concentration of component of fermention medium is: wheat bran hydrolyzed solution sugar 100g/L, and ammonium sulfate 2g/L, yeast soaks powder 3g/L, potassium primary phosphate 0.1g/L, dipotassium hydrogen phosphate 0.2g/L, magnesium sulfate 0.1g/L, manganous sulfate 0.1g/L, ferrous sulfate 0.05g/L, calcium carbonate 70g/L.
1000mL fermention medium is added in 10 250mL triangular flasks to each triangular flask 100mL.Put into sterilizing 20min at 121 ℃ of Autoclaves.
D, the preparation of described aspergillus tubigensis seed culture fluid
Method is as follows: be 1 × 10 by concentration
6it is in 10:1～15:1 seed culture medium that the aspergillus spore liquid of CFU/mL is linked into its volume ratio, under 30 ℃～32 ℃, 180r/min～200r/min condition, cultivates 16～18h, obtains concentration for (1～10) × 10
4the aspergillus tubigensis seed culture fluid of CFU/mL; Wherein, described seed culture medium calculates and comprises following composition by 1L: glucose 30g, ammonium sulfate 1.9g～2.2g, yeast soak powder 2.7g～3.3g, potassium primary phosphate 0.1g～0.15g, dipotassium hydrogen phosphate 0.2g～0.3g, magnesium sulfate 0.1g～0.2g, manganous sulfate 0.1g～0.2g, ferrous sulfate 0.05g～0.07g.
Wherein, fermented bacterium used is aspergillus (Aspergillusparasiticus) CICC40365, is purchased from Chinese industrial microbial strains preservation center.
Oxysuccinic acid is produced in E, fermentation
By accessing in each triangular flask after 5mL aspergillus tubigensis seed culture fluid, be placed in incubator, 32 ℃ of temperature, rotating speed 200r/min condition bottom fermentation 5 days, in gained fermented liquid, product oxysuccinic acid exists with the form of calcium salt, and malic acid concentration reaches 52.14g/L.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (5)
1. a method of utilizing the fermentation of Testa Tritici hydrolysis sugar to produce oxysuccinic acid, is characterized in that comprising the following steps:
(1) sulphuric acid soln that is 2%～4% by Testa Tritici powder and mass concentration is 1g:(7.5～12.5 by mass volume ratio) ml mixes, and at 121 ℃, reacts 1h, cooling, filter, and gets filtrate; In filtrate, add excess amount of Ca (OH)
2regulate pH to 10～11, under 8000r/min condition, centrifugal 10min, gets supernatant liquor, in described supernatant liquor, adds acid solution adjusting pH to neutral, and under 8000r/min condition, centrifugal 10min, gets supernatant liquor, obtains Testa Tritici hydrolysis sugar liquid;
(2) toward the gac that is incorporated as its quality 10% in described Testa Tritici hydrolysis sugar liquid, detoxification 1h under 40 ℃ of bath temperatures, 120r/min hunting speed, by reacting liquid filtering, gets filtrate, obtaining the liquid glucose that ferments, is 100g/L by described fermentation liquid glucose evaporation concentration to total sugar concentration;
(3), after fermentation liquid glucose step (2) being obtained mixes with nutritive substance, sterilizing 20min at 121 ℃ of temperature, obtains fermentation culture; Wherein, after described fermentation liquid glucose mixes with nutritive substance, cumulative volume calculates by 1L, and the quality of described fermentation liquid glucose and each nutritive substance is respectively: fermentation liquid glucose 100g～110g, ammonium sulfate 1.9g～2.2g, yeast soak powder 2.7g～3.3g, potassium primary phosphate 0.1g～0.15g, dipotassium hydrogen phosphate 0.2g～0.3g, magnesium sulfate 0.1g～0.2g, manganous sulfate 0.1g～0.2g, ferrous sulfate 0.05g～0.07g, calcium carbonate 70g～100g;
(4) add (1～10) × 10 toward the fermentation culture in step (3)
4cFU/mL aspergillus tubigensis seed culture fluid, 32 ℃, air air flow 2.5～30L/min, stirring velocity 160r/min condition bottom fermentation 5 days, collects fermented liquid, obtains the oxysuccinic acid existing with the form of calcium salt; Wherein, the volume ratio of described fermentation culture and aspergillus tubigensis seed culture fluid is 1:10.
2. the method for utilizing the fermentation of Testa Tritici hydrolysis sugar to produce oxysuccinic acid as claimed in claim 1, it is characterized in that, in step (1), described Testa Tritici powder is prepared by following steps: Testa Tritici is dried at 80 ℃ of temperature, Testa Tritici after drying is pulverized, crossed 60 mesh sieves, obtain Testa Tritici powder.
3. the method for utilizing the fermentation of Testa Tritici hydrolysis sugar to produce oxysuccinic acid as claimed in claim 2, is characterized in that, in step (1), described interpolation Ca (OH) 2 is pressed powder, analytical pure, purity >=95%; Described acid solution is the sulfuric acid of mass concentration 3%.
4. the method for utilizing the fermentation of Testa Tritici hydrolysis sugar to produce oxysuccinic acid as claimed in claim 3, it is characterized in that, in step (4), described aspergillus tubigensis seed culture liquid and preparation method thereof is as follows: it is in 10:1～15:1 seed culture medium that the aspergillus spore liquid that is 1 × 106CFU/mL by concentration is linked into its volume ratio, under 30 ℃～32 ℃, 180r/min～200r/min condition, cultivates 16～18h; Wherein, described seed culture medium calculates and comprises following composition by 1L: glucose 30g, ammonium sulfate 1.9g～2.2g, yeast soak powder 2.7g～3.3g, potassium primary phosphate 0.1g～0.15g, dipotassium hydrogen phosphate 0.2g～0.3g, magnesium sulfate 0.1g～0.2g, manganous sulfate 0.1g～0.2g, ferrous sulfate 0.05g～0.07g.
5. the method for utilizing the fermentation of Testa Tritici hydrolysis sugar to produce oxysuccinic acid as claimed in claim 4, is characterized in that, described aspergillus bacterial classification is aspergillus (Aspergillusparasiticus) CICC40365, is purchased from Chinese industrial microbial strains preservation center.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410036126.4A CN103789359B (en) | 2014-01-24 | 2014-01-24 | A kind of method utilizing the fermentation of Testa Tritici hydrolysis sugar to produce oxysuccinic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410036126.4A CN103789359B (en) | 2014-01-24 | 2014-01-24 | A kind of method utilizing the fermentation of Testa Tritici hydrolysis sugar to produce oxysuccinic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103789359A true CN103789359A (en) | 2014-05-14 |
| CN103789359B CN103789359B (en) | 2016-03-16 |
Family
ID=50665414
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410036126.4A Expired - Fee Related CN103789359B (en) | 2014-01-24 | 2014-01-24 | A kind of method utilizing the fermentation of Testa Tritici hydrolysis sugar to produce oxysuccinic acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103789359B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107699537A (en) * | 2017-12-05 | 2018-02-16 | 陈金华 | For cultivating marine green alga complex culture medium |
| CN108300739A (en) * | 2017-01-13 | 2018-07-20 | 山东阜丰发酵有限公司 | A kind of separation method for L MALIC ACID |
| CN113913472A (en) * | 2021-09-30 | 2022-01-11 | 巢湖学院 | Method for efficiently preparing succinic acid by stimulating corynebacterium crenatum fermentation bran hydrolysate with weak current |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108300740B (en) * | 2017-01-13 | 2021-08-03 | 山东阜丰发酵有限公司 | Method for preparing L-malic acid |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1112160A (en) * | 1994-05-20 | 1995-11-22 | 广东省微生物研究所 | High-yield saltant aspergillus N1-14' for L-malic acid and method for producing L-malic acid |
| CN102549163A (en) * | 2009-10-08 | 2012-07-04 | 帝斯曼知识产权资产管理有限公司 | Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars |
| CN102634545A (en) * | 2012-05-04 | 2012-08-15 | 苏州百趣食品有限公司 | Method for producing L-malic acid by fermenting sucrose molasses |
-
2014
- 2014-01-24 CN CN201410036126.4A patent/CN103789359B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1112160A (en) * | 1994-05-20 | 1995-11-22 | 广东省微生物研究所 | High-yield saltant aspergillus N1-14' for L-malic acid and method for producing L-malic acid |
| CN102549163A (en) * | 2009-10-08 | 2012-07-04 | 帝斯曼知识产权资产管理有限公司 | Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars |
| CN102634545A (en) * | 2012-05-04 | 2012-08-15 | 苏州百趣食品有限公司 | Method for producing L-malic acid by fermenting sucrose molasses |
Non-Patent Citations (2)
| Title |
|---|
| 郭娜: "小麦麸皮纤维降解糖化与分层利用", 《中国博士学位论文全文数据库工程科技I辑》, no. 04, 15 April 2014 (2014-04-15) * |
| 郭娜等: "小麦麸皮纤维稀酸水解糖化工艺研究", 《安徽农业科学》, vol. 40, no. 24, 31 December 2012 (2012-12-31), pages 12232 - 12234 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108300739A (en) * | 2017-01-13 | 2018-07-20 | 山东阜丰发酵有限公司 | A kind of separation method for L MALIC ACID |
| CN108300739B (en) * | 2017-01-13 | 2021-03-05 | 山东阜丰发酵有限公司 | Separation method for L-malic acid |
| CN107699537A (en) * | 2017-12-05 | 2018-02-16 | 陈金华 | For cultivating marine green alga complex culture medium |
| CN113913472A (en) * | 2021-09-30 | 2022-01-11 | 巢湖学院 | Method for efficiently preparing succinic acid by stimulating corynebacterium crenatum fermentation bran hydrolysate with weak current |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103789359B (en) | 2016-03-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104171759A (en) | Edible mushroom residue feed preparation process | |
| CN102633865B (en) | Method for pretreating enramycin fermentation liquor and method for preparing enramycin premix by using enramycin fermentation liquor | |
| CN102138663B (en) | Method for processing fermented type nutritional fruit and vegetable powder rich in vitamin | |
| CN104109622B (en) | Preparation method of selenium-rich edible vinegar | |
| CN103789359B (en) | A kind of method utilizing the fermentation of Testa Tritici hydrolysis sugar to produce oxysuccinic acid | |
| CN103749959A (en) | Biological fermented bran and processing method thereof | |
| CN103053978B (en) | Acete chinensis cooking liquid seafood seasoning and preparation method thereof | |
| CN104172080A (en) | Method for extracting amino acid from oyster meat | |
| CN103931776A (en) | Production process of bean dregs powder | |
| CN102154080A (en) | Preparation method of luzhou-flavour white spirit cellar mud added with esterified liquid | |
| CN109825403B (en) | Method for improving content of beta-phenethyl alcohol in rice wine | |
| CN107853588A (en) | A kind of preparation method of feature highland barley flour | |
| CN103642885A (en) | Method for producing wheat biological active peptide by fermentation | |
| CN105192663A (en) | Preparation method for black tea essence | |
| CN110973582A (en) | Preparation method of peanut meal soy sauce, peanut meal soy sauce prepared by method and application of peanut meal soy sauce | |
| CN105211630A (en) | Humic acid biological feed additive and production method | |
| CN111549080A (en) | Fermentation production process of zinc propionate | |
| CN104557154A (en) | Method for producing amino acid fertilizer from soybean meal through solid-state fermentation | |
| CN105861391A (en) | Normal temperature compound microbial fermentation agent | |
| CN107201383A (en) | It is a kind of to improve the D-ALPHA-Hydroxypropionic acid production method that D-ALPHA-Hydroxypropionic acid produces intensity | |
| CN110558536A (en) | Method for improving freshness of brewed soy sauce by microwave vacuum treatment | |
| CN102687796B (en) | Method for producing bioprotein feed using tomato pomace | |
| CN101701206B (en) | Method for producing microorganism culture media nitrogen source from waste organisms | |
| CN113966695A (en) | Hypsizigus marmoreus culture medium and preparation method and application thereof | |
| KR101534391B1 (en) | Compost type nutritional supplements for apple cultivation and process for preparing the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160316 Termination date: 20190124 |