CN106728422A - It is a kind of to prevent and treat pharmaceutical composition of glaucoma and preparation method thereof - Google Patents

It is a kind of to prevent and treat pharmaceutical composition of glaucoma and preparation method thereof Download PDF

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CN106728422A
CN106728422A CN201611133182.5A CN201611133182A CN106728422A CN 106728422 A CN106728422 A CN 106728422A CN 201611133182 A CN201611133182 A CN 201611133182A CN 106728422 A CN106728422 A CN 106728422A
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pharmaceutical composition
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Jinan Haoyu Qingtian Medicine Technology Co ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/19—Acanthaceae (Acanthus family)
    • A61K36/195—Strobilanthes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/82—Theaceae (Tea family), e.g. camellia
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/83—Thymelaeaceae (Mezereum family), e.g. leatherwood or false ohelo
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/55—Liquid-liquid separation; Phase separation

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Abstract

Pharmaceutical composition of glaucoma and preparation method thereof is prevented and treated the invention discloses a kind of, pharmaceutical composition of the present invention is with Huang Ruimu, Daphne tangutica, saikosaponin C, Strobilanthes aehobarbus W. W. Smith, common anotis herb as bulk drug, proportioning is formed, routinely various formulations can be made by preparation process, prevent and treat glaucoma evident in efficacy.

Description

It is a kind of to prevent and treat pharmaceutical composition of glaucoma and preparation method thereof
Technical field
The invention belongs to technical field of traditional Chinese medicines, more particularly to a kind of pharmaceutical composition for preventing and treating glaucoma and its preparation side Method.
Background technology
The Hemodynamics research of diabetic has shown the dysfunction of the retina blood capillary of diabetic, Hardening and obturation are the basic reasons for causing retinal blood dyshaemia.The view of diabetes non-proliferative phase patient with retinopathy The VPV of film central artery is lower than normal person, and drag index increases with PI, vascular resistance index reflection blood vessel Hemodynamic characteristics, index is high to show that distal vessels bed resistance is big, and CBF is few, and diabetic's blood is in high glutinous State.Increase or during HT state when certain factor promotes retina oxygen demand, can induce or to aggravate diabetic eye micro- Circulate generation, the development of lesion.
Recent studies indicate that glaucoma morbidity in addition to anatomical factors, also physiologic factor the reason for.Wherein choroid Innermost layer be capillary layer, be characterized in that caliber is especially big, be 4 times of normal terminal vessels, and blood capillary herein The pressure of pipe is higher.It is estimated that intraocular blood has 70% to be stored in CC.Capillary is in fenestra Type, basement membrane is thin, therefore it has stronger permeability, as the choroid of one of the maximum organ of unit CBF in body, Its blood flow lacks capacity of self-regulation.
Diabetic is receiving anti-glaucoma surgery, the aggravation of eye Micro-circutation syndrome.For a long time, made with glucocorticoid It is main treatment method, the approach of arachidonic acid generation can be blocked because of it, generates prostaglandin few, vascular permeability Property decline, and lower because of the increased vascular endothelial growth factor of its vasopermeability, mitigate the broken of blood retina barrier It is bad.But diabetic makes oculist in a dilemma using the blood glucose rise phenomenon after hormone therapy.The diabetes spp traditional Chinese medical science " quenching one's thirst " category is learned, its interpretation of the cause, onset and process of an illness is " deficiency of Yin is this, and scorching is mark ", dry to hinder body fluid, kidney deficiency and liver, deficiency of both qi and yin, the deficiency of vital energy The blood stasis of blood is stagnant." glaucoma " traditional Chinese medical science is referred to as " bluish glaucoma ", belongs to caused by liver and kidney deficiency, liver-yang hyperactivity, the bright body fluid of heat, poly- liquid into Phlegm, stasis blocking train of thought is qi-blood disharmony.Traditional Chinese medical science surgery trauma is called " incised wound ", causes local zang-fu differentiation qi and blood unbalance, is made Damaged into train of thought, so as to further aggravate qi and blood stagnation.
Daphne tangutica：This product is the leaf of Isolated From Thymelaeaceae Species Daphne tangutica Daphne tangutica Maxim., stem skin, Really, flower.Harvested respectively at flower, fruiting period, remove impurity, dry.【Nature and flavor】It is puckery, put down.【Function with cure mainly】Clearing damp, desinsection.Fruit For indigestion, parasitosis；Leaf, branch endure cream and control parasitosis；Stem skin cream controls arthritis with fixed pain caused by dampness, joint product yellow water.【Proterties】This product spends several clusters It is born on short floral axis, or it is single flower to come off；Bract edge has eyelashes；Calyx tube is thin, and tip four splits floweriness flap, and drying shrinkage bends, 2cm long, surface yellowish-brown to dark brown.Stem is flexible, and tender skin is orange-yellow；The silver grey black of Lao Pi, has fine wrinkle and horizontal hole skin, easily Peel off, inner face yellow green.Leaf keratin, focuses mostly under inflorescence, and strip lanceolar or round shape long are oval, surface light green color tool Fine wrinkle, the back side is light brown, and master pulse is obvious, edge warp.The black purple of drupe, drying shrinkage, carpopodium tool hair.Gas is faint, sweet, micro- It is pungent.Record and issue Tibetan medicine standard in the Ministry of Public Health（First）, standard number：WS3-BC-0024-95.
Huang Ruimu：This product is the yellow auspicious wooden Adinandra millettii of Theaceae Yang Tong platymisciums（Hook.et Arn.） The root and tender leaf of Benth.et Hook.f..Root whole year can adopt, and dry.Tender leaf summer, Qiu Cai, using fresh herb.【Nature and flavor】It is bitter；It is cool.【Return Through】Lung；Liver Channel.【Indication】Cooling blood and hemostasis；Removing toxicity for detumescence.Main bleeding from five sense organs or subcutaneous tissue；Hematuria；Catarrhal jaundice；Parotitis；Furuncle；Snake Insect bite；Cancerous swelling.【Former phytomorph】Yellow auspicious wooden shrub or dungarunga, about 5m high.Spray and terminal bud sparsely grow pubescence.Single leaf is mutual It is raw；Leaf has short handle；Blade heavy leather matter, round shape long is oval, and 4.5-9cm long, 2-3cm wide, the short point of tip, base portion are gradually narrow, edge Full edge, it is rare to slightly have denticulation in the first half, there is intensive pubescence when young, become afterwards without hair.Flower both sexes, are singly born in axil；Bennet It is very thin, 2cm is about, there is patch volt undercoat, undercoat is lied prostrate in sepal 5, ovate triangle, outside by patch, and edge is bordering on film quality, there is thin gland tooth And eyelashes；Corolla lobe 5, without hair；About 25 pieces of stamen, the close raw white pubescence of flower pesticide；Ovary is upper, Room 3, there is white pubescence, flower Post is without hair.Berry is subsphaeroidal, diameter 7-8mm, has pubescence or is bordering on without hair.Seed is tiny, black, light.Record big in Chinese medicine Dictionary.
Strobilanthes aehobarbus W. W. Smith：It is Acanthaceae kalimeris platymiscium capillary copper purple cloud dish Strobilanthes aehobarbus W. W. The root and leaf of Smith.Root, leaf whole year can adopt, and clean, and dry.【Nature and flavor】It is lightly seasoned；Slight bitter；It is mild-natured.【Return through】The heart；Lung；Liver Through.【Indication】Clearing liver dampness removing；Promoting blood circulation to remove blood stasis.Main jaundice with damp-heat pathogen；Red eye, swell pain；Irregular menstruation；Postpartum abdominal pain.【Former plant Form】The capillary copper purple cloud perennial shrub shape draft of dish, about 1m high.Stem is upright, multi-branched, the prismatic of top four, and section expands, flat It is flat, it is close by brown color undercoat, it is miscellaneous to have white pubescence long, it is especially close with browse hair.Single leaf opposite；There is handle；Blade oval or ovum Shape, tip is tapering, and base portion is slightly asymmetric, blunt circle or heart, and side tool dredges crenation, above sunlight green, below mulberry, two sides quilt Brown color undercoat and white become mildewed, in blueness after hair such as wiping is de- on branches and leaves.Spike basidixed or axillary；Bract is lobate；Calyx 5 Split；Flower purple；Corolla tubular, tip 5 splits, two lips；Stamen 4, the last two, filigree base portion has river film quality to be connected, and corolla inner face has 2 Row undercoat.Capsule.There is 4, seed.Record in dictionary of medicinal plant.
Common anotis herb：It is Rubiaceae vacation plants of Hedyotis common anotis herb Anotis ingrata (wall.) Hook. F. [Neanotis Yngrata (Wall.ex Hook .f) W.H.Lewis] herb.Summer, autumn receive and adopt herb, eliminate soil, debris, dry It is standby.【Nature and flavor】Taste is pungent；It is cool in nature.【Return through】The heart；Liver Channel.【Indication】Clearing liver-fire.Main nameless sores or boils；Hot eyes is red and swollen；Poison Snake bite.【Former phytomorph】Renascent herb, up to 1m.Stem branch or does not have a small number of branches, the sleeping ground in upright or bottom.Leaf Opposite；Petiole is short；The nearly triangle in stipule bottom, joint is born between two petioles, edge tool fine hair；Blade papery, ovum Circular or oval long is to ovum shape lanceolar, and 4-9cm long, about 1.8cm wide, tip is tapering, base portion wedge shape, full edge, two sides quilt Pubescence.Dichasium basidixed, total bennet and branch have narrow aliform corner angle；Floral white, without obstructing or have short stalk；Calyx 4 Split, sliver triangular shape lanceolar, corolla is about 5mm, hat manages slightly wider, interior raw pubescence；Stamen 4, flower pesticide exposes outside corolla；Under ovary Position, Room 2, column cap 2 splits.Capsule is subsphaeroidal, slightly flat-sided, about 2mm wide.Seed is small and most, plano-convex, there is small wart point.Florescence 6-7 Month, the fruiting period 7-8 months.Record in dictionary of medicinal plant.
Saikosaponin C（Saikosaponin C）：CAS 20736-08-7, molecular formula C48H78O17, molecular weight 927.12. 【Ingredient origin】Radix bupleuri Bupleurum chinense, RADIX BUPLEURI SCORZONERIFOLII Bupleurum scorzonerifolium.
Saikosaponin C（Saikosaponin C）.
The content of the invention
The purpose of the present invention is to overcome the shortcomings of background technology, there is provided it is a kind of effectively prevent and treat glaucoma pharmaceutical composition and Its preparation method.
The present invention adopts the following technical scheme that realization：
The composition and weight portion for being made the bulk drug of the pharmaceutical composition for preventing and treating glaucoma be：
Yellow auspicious wooden 356-358 weight portions Daphne tangutica 360-364 weight portions saikosaponin C 12-14 weight portion Strobilanthes aehobarbus W. W. Smiths 350-352 weight portion common anotis herb 330-336 weight portions.
The pharmaceutical composition for preventing and treating glaucoma is preferably used in, is made up of the bulk drug of following weight portion：
The weight portion one of 13 weight portion Strobilanthes aehobarbus W. W. Smith of yellow 362 weight portion saikosaponin C of auspicious wooden 357 weight portion Daphne tangutica 351 Post 333 weight portions of perfume.
A kind of pharmaceutical composition for preventing and treating glaucoma, it is characterised in that pharmaceutical composition can be using the routine side of galenic pharmacy Method prepares piece agent or capsule or dripping pill.
A kind of pharmaceutical composition for preventing and treating glaucoma, it is characterised in that pharmaceutical composition is anti-with what chemical drugs or Chinese medicine were constituted Control glaucoma medicine.
A kind of preparation method of the pharmaceutical composition for preventing and treating glaucoma, it is characterised in that prepare as follows：
The composition and weight portion of bulk drug be：Yellow auspicious wooden 356-358 weight portions Daphne tangutica 360-364 weight portion saikosides C12-14 weight portion Strobilanthes aehobarbus W. W. Smith 350-352 weight portion common anotis herb 330-336 weight portions；
Preparation method：
（1）Huang Ruimu, Daphne tangutica, saikosaponin C, Strobilanthes aehobarbus W. W. Smith, common anotis herb are taken by bulk drug proportioning, is mixed, with weight hundred Divide the ethanol of specific concentration 36% as solvent, taken in 34 DEG C of temperature extractions, extraction time is 10 times, and each extraction time is 17 hours, often Secondary solvent load is 65 times of bulk drug gross weight, is filtered, and obtains dregs of a decoction A and extract solution A, and extract solution A reclaims ethanol, is concentrated into phase To density 1.16, filtration, liquid is first washed with water by DM20 large pore resin absorption columns, then with the second of weight percent concentration 27% Alcoholic solution elutes DM20 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 27%, reclaims ethanol, and concentration is dry It is dry, obtain final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 46% as solvent, heating and refluxing extraction 15 times is carried every time The time is taken for 0.3 hour, each solvent load is 39 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned Ethanol is received, relative density 1.02 is concentrated into, filtered, liquid is first washed with water, then use weight by H130 large pore resin absorption columns The ethanol solution of percent concentration 71% elutes H130 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 71%, Ethanol is reclaimed, concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
Preferred a kind of preparation method of the pharmaceutical composition for preventing and treating glaucoma, it is characterised in that prepare as follows：
The composition and weight portion of bulk drug be：The weight of 362 weight portion saikosaponin C of yellow auspicious wooden 357 weight portion Daphne tangutica 13 The weight portion of 351 weight portion common anotis herb of part Strobilanthes aehobarbus W. W. Smith 333；
Preparation method：
（1）Huang Ruimu, Daphne tangutica, saikosaponin C, Strobilanthes aehobarbus W. W. Smith, common anotis herb are taken by bulk drug proportioning, is mixed, with weight hundred Divide the ethanol of specific concentration 36% as solvent, taken in 34 DEG C of temperature extractions, extraction time is 10 times, and each extraction time is 17 hours, often Secondary solvent load is 65 times of bulk drug gross weight, is filtered, and obtains dregs of a decoction A and extract solution A, and extract solution A reclaims ethanol, is concentrated into phase To density 1.16, filtration, liquid is first washed with water by DM20 large pore resin absorption columns, then with the second of weight percent concentration 27% Alcoholic solution elutes DM20 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 27%, reclaims ethanol, and concentration is dry It is dry, obtain final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 46% as solvent, heating and refluxing extraction 15 times is carried every time The time is taken for 0.3 hour, each solvent load is 39 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned Ethanol is received, relative density 1.02 is concentrated into, filtered, liquid is first washed with water, then use weight by H130 large pore resin absorption columns The ethanol solution of percent concentration 71% elutes H130 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 71%, Ethanol is reclaimed, concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
A kind of preparation method of the pharmaceutical composition for preventing and treating glaucoma, it is characterised in that pharmaceutical composition can use preparation Conventional method prepares piece agent or capsule or dripping pill.
A kind of preparation method of the pharmaceutical composition for preventing and treating glaucoma, it is characterised in that pharmaceutical composition and chemical drugs or in Medicine composition preventing and treating glaucoma medicine.
It is evident in efficacy that pharmaceutical composition prevents and treats glaucoma.
Specific embodiment
Embodiment 1：Prevent and treat pharmaceutical composition of glaucoma and preparation method thereof
The composition and weight portion for preventing and treating the bulk drug of the pharmaceutical composition of glaucoma be：Yellow auspicious wooden 357g Daphne tanguticas 362g bavins Hu saponin(e C13g Strobilanthes aehobarbus W. W. Smith 351g common anotis herbs 333g；
Preparation method：
（1）Huang Ruimu, Daphne tangutica, saikosaponin C, Strobilanthes aehobarbus W. W. Smith, common anotis herb are taken by bulk drug proportioning, is mixed, with weight hundred Divide the ethanol of specific concentration 36% as solvent, taken in 34 DEG C of temperature extractions, extraction time is 10 times, and each extraction time is 17 hours, often Secondary solvent load is 65 times of bulk drug gross weight, is filtered, and obtains dregs of a decoction A and extract solution A, and extract solution A reclaims ethanol, is concentrated into phase To density 1.16, filtration, liquid is first washed with water by DM20 large pore resin absorption columns, then with the second of weight percent concentration 27% Alcoholic solution elutes DM20 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 27%, reclaims ethanol, and concentration is dry It is dry, obtain final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 46% as solvent, heating and refluxing extraction 15 times is carried every time The time is taken for 0.3 hour, each solvent load is 39 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned Ethanol is received, relative density 1.02 is concentrated into, filtered, liquid is first washed with water, then use weight by H130 large pore resin absorption columns The ethanol solution of percent concentration 71% elutes H130 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 71%, Ethanol is reclaimed, concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
Embodiment 2：Prevent and treat pharmaceutical composition of glaucoma and preparation method thereof
The composition and weight portion for preventing and treating the bulk drug of the pharmaceutical composition of glaucoma be：Yellow auspicious wooden 356g Daphne tanguticas 364g bavins Hu saponin(e C12g Strobilanthes aehobarbus W. W. Smith 352g common anotis herbs 330g；
Preparation method：
（1）Huang Ruimu, Daphne tangutica, saikosaponin C, Strobilanthes aehobarbus W. W. Smith, common anotis herb are taken by bulk drug proportioning, is mixed, with weight hundred Divide the ethanol of specific concentration 36% as solvent, taken in 34 DEG C of temperature extractions, extraction time is 10 times, and each extraction time is 17 hours, often Secondary solvent load is 65 times of bulk drug gross weight, is filtered, and obtains dregs of a decoction A and extract solution A, and extract solution A reclaims ethanol, is concentrated into phase To density 1.16, filtration, liquid is first washed with water by DM20 large pore resin absorption columns, then with the second of weight percent concentration 27% Alcoholic solution elutes DM20 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 27%, reclaims ethanol, and concentration is dry It is dry, obtain final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 46% as solvent, heating and refluxing extraction 15 times is carried every time The time is taken for 0.3 hour, each solvent load is 39 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned Ethanol is received, relative density 1.02 is concentrated into, filtered, liquid is first washed with water, then use weight by H130 large pore resin absorption columns The ethanol solution of percent concentration 71% elutes H130 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 71%, Ethanol is reclaimed, concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
Embodiment 3：Prevent and treat pharmaceutical composition of glaucoma and preparation method thereof
The composition and weight portion for preventing and treating the bulk drug of the pharmaceutical composition of glaucoma be：Yellow auspicious wooden 358g Daphne tanguticas 360g bavins Hu saponin(e C14g Strobilanthes aehobarbus W. W. Smith 350g common anotis herbs 336g；
Preparation method：
（1）Huang Ruimu, Daphne tangutica, saikosaponin C, Strobilanthes aehobarbus W. W. Smith, common anotis herb are taken by bulk drug proportioning, is mixed, with weight hundred Divide the ethanol of specific concentration 36% as solvent, taken in 34 DEG C of temperature extractions, extraction time is 10 times, and each extraction time is 17 hours, often Secondary solvent load is 65 times of bulk drug gross weight, is filtered, and obtains dregs of a decoction A and extract solution A, and extract solution A reclaims ethanol, is concentrated into phase To density 1.16, filtration, liquid is first washed with water by DM20 large pore resin absorption columns, then with the second of weight percent concentration 27% Alcoholic solution elutes DM20 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 27%, reclaims ethanol, and concentration is dry It is dry, obtain final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 46% as solvent, heating and refluxing extraction 15 times is carried every time The time is taken for 0.3 hour, each solvent load is 39 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned Ethanol is received, relative density 1.02 is concentrated into, filtered, liquid is first washed with water, then use weight by H130 large pore resin absorption columns The ethanol solution of percent concentration 71% elutes H130 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 71%, Ethanol is reclaimed, concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
Embodiment 4：The preparation of tablet
The pharmaceutical composition 73g of Example 1, adds starch 124g, mixes, and granulation is dried, plus microcrystalline cellulose 34g, stearic acid Magnesium 6g, mixes, and is pressed into 500, obtains final product medicinal composition tablets.
Embodiment 5：The preparation of capsule
The pharmaceutical composition 62g of Example 2, adds starch 180g, mixes, and granulation is dried, whole grain, adds appropriate magnesium stearate, Mix, encapsulated 520, obtain final product medicament composition capsule.
Embodiment 6：The preparation of dripping pill
Weigh (80 DEG C) heating of Macrogol 6000 310g water-baths and boil molten, add the pharmaceutical composition 22g of embodiment 3, fully stir Mix uniform, with atoleine as cooling agent, put glass tube（4*80cm）In, chilling temperature is -4 DEG C, and drip internal-and external diameter is 7.0/ 2.0 (mm/mm), drip is 2.2cm away from liquid level, and drop speed is optimum condition with every point 58 drop, and the cold of dripping pill surface is blotted with cotton Solidifying agent, obtains final product medicament composition dropping pills.
Embodiment 7：Prevent and treat the pharmaceutical composition of glaucoma
The composition and weight portion for preventing and treating the bulk drug of the pharmaceutical composition of glaucoma be：
The weight portion of 365 weight portion common anotis herb of yellow auspicious wooden 383 weight portion Strobilanthes aehobarbus W. W. Smith 340.
Embodiment 8：Prevent and treat the pharmaceutical composition of glaucoma
The composition and weight portion for preventing and treating the bulk drug of the pharmaceutical composition of glaucoma be：
The weight portion of 12 weight portion Strobilanthes aehobarbus W. W. Smith of saikosaponin C, 136 weight portion common anotis herb 250.
Embodiment 9：Prevent and treat the pharmaceutical composition of glaucoma
The composition and weight portion for preventing and treating the bulk drug of the pharmaceutical composition of glaucoma be：
The weight portion of 14 weight portion Strobilanthes aehobarbus W. W. Smith of saikosaponin C, 350 weight portion common anotis herb 345.
Experimental example 1：Prevent and treat the experimental study of glaucoma
1 data and method
1.1 data
Diabetes mellitus glaucoma patient 34 (51) is this city hospital 2012 year and was in hospital 2 months in December, 2012 execution glaucoma The postoperative patient of Trabecula resection, wherein Chinese medicine group 17 (26), the people of male 9, the people of women 8；Average age 60.5 years old；II type sugar Urinate the people of disease 14, the people of IDDM 3；The people of open-angle glaucoma 3, the people of angle-closure glaucoma 14.Western medicine group 17 (25), wherein The people of male 9, the people of women 8；Average age 60.5 years old；The people of type II diabetes 14, the people of IDDM 3；The people of open-angle glaucoma 3, closes The people of angle-style glaucoma 13, the people of other types glaucoma 1.All diabetics make a definite diagnosis through division of endocrinology of our hospital, diabetes medical history It is different in size, preoperative fasting blood-glucose≤8.0mmol/L, preoperative intraocular pressure≤35mmHg.It is statistically analyzed, two groups of patients exist Sex, age, the course of disease, there are no significant in state of an illness difference, with comparativity.
1.2 diagnostic criteria
Diabetic makes a definite diagnosis according to the standard that WHO is announced.Glaucoma patient meets ophthalmology, hals,Nasen und Ohrenheilkunde conventional treatment and examines Disconnected standard and the person that meets surgical condition.
1.3 operation methods
All patients suture scleral flap by same position eye doctor routinely row trabeculectomy.
1.4 aftertreatments
1.4.1 Chinese medicine group
Operation completes to give the pharmaceutical composition of soothing the liver enriching yin, nourishing blood for improving eyesight from this very day（The pharmaceutical composition of embodiment 1）, every time 1g, it is oral that 3 times a day, and oral indometacin 25mg, and 3 times a day, is within continuous 14 days 1 course for the treatment of.
1.4.2 Western medicine group
Operation completes to give the physiological saline 250ml+ methylate prednisolone 40mg of drip-feed 0.9%, clothes of being withdrawn the previous remark after 1 week from this very day Prednisone 30mg, one time a day, the mg of tapered dosage 5, totally 1 week.
Two groups of Topical corticosteroid ocular fluid and cycloplegics from postoperative 1st day.
1.5 observation items
The eyesight fluctuation of postoperative patients；Intraocular pressure；The visual field；Optic disk cup disc ratio；The depth of anterior chamber；Inflammatory reaction；Corneal endothelium shape State；Filtration bleb.
2 results
2.1 efficacy assessment standards
Drafted with reference to ophthalmology, hals,Nasen und Ohrenheilkunde conventional treatment.Cure:Intraocular pressure recovers normal range (NR), and the visual field, eyeground infringement are controlled System.Take a turn for the better:Intraocular pressure declines, and the visual field, eyeground are unchanged.Do not heal:Intraocular pressure does not drop, and the visual field continues to zoom out, eyeground infringement aggravation.
2.2 total effectses
Cure 12 in Chinese medicine group, take a turn for the better 12, not more 2, total effective rate is 92.31%.Western medicine group cures 9, improvement 10 Eye, not more 6, total effective rate is 76.0% (P<0.05).
2.3 two groups of clinical symptoms changes (table 1)
Two groups of changes (n) of clinical symptoms after the treatment of table 1
Pharmaceutical composition improves to clinical symptoms and is better than control group.

Claims (8)

1. a kind of pharmaceutical composition for preventing and treating glaucoma, it is characterised in that be made the composition and weight of the bulk drug of the pharmaceutical composition Measuring part is：
Yellow auspicious wooden 356-358 weight portions Daphne tangutica 360-364 weight portions saikosaponin C 12-14 weight portion Strobilanthes aehobarbus W. W. Smiths 350-352 weight portion common anotis herb 330-336 weight portions.
2. a kind of pharmaceutical composition for preventing and treating glaucoma according to claim 1, it is characterised in that be made the pharmaceutical composition Bulk drug composition and weight portion be：
The weight portion one of 13 weight portion Strobilanthes aehobarbus W. W. Smith of yellow 362 weight portion saikosaponin C of auspicious wooden 357 weight portion Daphne tangutica 351 Post 333 weight portions of perfume.
3. a kind of pharmaceutical composition for preventing and treating glaucoma according to claim 1, it is characterised in that pharmaceutical composition can be adopted Piece agent or capsule or dripping pill are prepared with the conventional method of galenic pharmacy.
4. a kind of pharmaceutical composition for preventing and treating glaucoma according to claim 1, it is characterised in that pharmaceutical composition and chemistry The preventing and treating glaucoma medicine of medicine or Chinese medicine composition.
5. a kind of preparation method of the pharmaceutical composition for preventing and treating glaucoma, it is characterised in that prepare as follows：
The composition and weight portion of bulk drug be：Yellow auspicious wooden 356-358 weight portions Daphne tangutica 360-364 weight portion saikosides C12-14 weight portion Strobilanthes aehobarbus W. W. Smith 350-352 weight portion common anotis herb 330-336 weight portions；
Preparation method：
（1）Huang Ruimu, Daphne tangutica, saikosaponin C, Strobilanthes aehobarbus W. W. Smith, common anotis herb are taken by bulk drug proportioning, is mixed, with weight hundred Divide the ethanol of specific concentration 36% as solvent, taken in 34 DEG C of temperature extractions, extraction time is 10 times, and each extraction time is 17 hours, often Secondary solvent load is 65 times of bulk drug gross weight, is filtered, and obtains dregs of a decoction A and extract solution A, and extract solution A reclaims ethanol, is concentrated into phase To density 1.16, filtration, liquid is first washed with water by DM20 large pore resin absorption columns, then with the second of weight percent concentration 27% Alcoholic solution elutes DM20 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 27%, reclaims ethanol, and concentration is dry It is dry, obtain final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 46% as solvent, heating and refluxing extraction 15 times is carried every time The time is taken for 0.3 hour, each solvent load is 39 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned Ethanol is received, relative density 1.02 is concentrated into, filtered, liquid is first washed with water, then use weight by H130 large pore resin absorption columns The ethanol solution of percent concentration 71% elutes H130 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 71%, Ethanol is reclaimed, concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
6. a kind of preparation method of the pharmaceutical composition for preventing and treating glaucoma according to claim 5, it is characterised in that by as follows It is prepared by step：
The composition and weight portion of bulk drug be：The weight of 362 weight portion saikosaponin C of yellow auspicious wooden 357 weight portion Daphne tangutica 13 The weight portion of 351 weight portion common anotis herb of part Strobilanthes aehobarbus W. W. Smith 333；
Preparation method：
（1）Huang Ruimu, Daphne tangutica, saikosaponin C, Strobilanthes aehobarbus W. W. Smith, common anotis herb are taken by bulk drug proportioning, is mixed, with weight hundred Divide the ethanol of specific concentration 36% as solvent, taken in 34 DEG C of temperature extractions, extraction time is 10 times, and each extraction time is 17 hours, often Secondary solvent load is 65 times of bulk drug gross weight, is filtered, and obtains dregs of a decoction A and extract solution A, and extract solution A reclaims ethanol, is concentrated into phase To density 1.16, filtration, liquid is first washed with water by DM20 large pore resin absorption columns, then with the second of weight percent concentration 27% Alcoholic solution elutes DM20 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 27%, reclaims ethanol, and concentration is dry It is dry, obtain final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 46% as solvent, heating and refluxing extraction 15 times is carried every time The time is taken for 0.3 hour, each solvent load is 39 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned Ethanol is received, relative density 1.02 is concentrated into, filtered, liquid is first washed with water, then use weight by H130 large pore resin absorption columns The ethanol solution of percent concentration 71% elutes H130 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 71%, Ethanol is reclaimed, concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
7. a kind of preparation method of the pharmaceutical composition for preventing and treating glaucoma according to claim 5, it is characterised in that medicine group Compound can prepare piece agent or capsule or dripping pill using the conventional method of galenic pharmacy.
8. a kind of preparation method of the pharmaceutical composition for preventing and treating glaucoma according to claim 5, it is characterised in that medicine group Compound and chemical drugs or Chinese medicine composition preventing and treating glaucoma medicine.
CN201611133182.5A 2016-12-10 2016-12-10 It is a kind of to prevent and treat pharmaceutical composition of glaucoma and preparation method thereof Withdrawn CN106728422A (en)

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