CN106721762B - Energy beverage - Google Patents

Abstract

The invention belongs to the field of functional beverages, and particularly relates to an energy beverage which is prepared from the following raw materials in 50 ml: total saponins of ginseng: 150-220 mg, wolfberry: 0.8-1.2 g, mint: 0.7-1.1 g, kola fruit: 0.8-1.2 g, taurine: 0.6-1.2 g, tyrosine: 160-210 mg, phenylalanine: 160-220 mg, niacin: 20-40 mg, folic acid: 400-700 micrograms, vitamin B6: 4-8 mg and vitamin B12: 18-22 micrograms, adding pure water to 50 ml. The invention can realize the aim of resisting fatigue and concentrating spirit by reasonably matching the components and exerting the synergistic effect; experiments also show that the energy beverage is safe and effective, and the anti-fatigue effect can reach more than 4 hours.

Description

Energy beverage

Technical Field

The invention belongs to the field of functional beverages, and particularly relates to an anti-fatigue high-efficiency energy beverage.

Background

Drowsiness and fatigue can affect driving. A plurality of studies show that the phenomenon of temporary sleep occurs in 14.5 to 20 percent of long-distance driving^[1](Van L aar et al 2008.) monotonous highway environment and few vehicles, tiredness and lack of sleep of drivers are the main causes of driving sleep, 15-23% of all traffic accidents are caused by dozing in driving^[2](Maycock 1996). Therefore, it is very meaningful to study the efficacy and safe driving hooks of energy drinks.

In driving, the following often occur if tired: (1) the speed fluctuation, such as on a road with the situation of 100 kilometers per hour, requires that a driver does not need to cruise, and the driving speed is controlled to be 95 kilometers per hour in a stepping-on mode. If the driver is tired, the speed of the vehicle can fluctuate above and below the specified speed, and the more tired the speed fluctuates, the more the speed fluctuates; (2) lateral shifting, when the driver is stranded, cannot keep driving in the center of the road, or left or right, and the more stranded the more severe the deviation. Both of the above criteria are very sensitive. There are two speed measuring instruments for driving fatigue.

The red cattle anti-fatigue beverage sold in the market at present consists of caffeine, taurine, lysine, inositol, vitamin PP (or nicotinamide), vitamin B6, vitamin B12, citric acid, sodium benzoate, lemon yellow, carmine, white granulated sugar and water. Has a certain anti-fatigue effect, but is not particularly effective in reducing the fatigue feeling caused by driving for a long time. Therefore, there is a need for a functional beverage that can be developed with a focus on fatigue resistance to achieve safe driving goals.

Disclosure of Invention

In view of the above-mentioned drawbacks and deficiencies of the prior art, it is an object of the present invention to provide a functional beverage that is hooked up to safe driving.

Summary of The Invention

An energy beverage is prepared from the following raw materials in 50 ml:

total saponins of ginseng: 150-220 mg, wolfberry: 0.8-1.2 g, mint: 0.7-1.1 g, kola fruit: 0.8-1.2 g, taurine: 0.6-1.2 g, tyrosine: 160-210 mg, phenylalanine: 160-220 mg, niacin: 20-40 mg, folic acid: 400-700 micrograms, vitamin B6: 4-8 mg and vitamin B12: 18-22 micrograms, adding pure water to 50 ml.

Preferably, the energy beverage is added in the form of plant extract. The conventional method is to add water into the medlar, boil the medlar for 30 minutes, and remove the slag for use.

Preferably, the energy beverage according to the present invention, wherein the mint is added in the form of a plant extract. The conventional method is to boil the mint in water for 30 minutes and remove the residue for use.

Preferably, the energy beverage is added in the form of plant extract. The conventional method is to boil the kola fruit in water for 30 minutes and remove the residue for use.

Detailed Description

The invention relates to an energy beverage which is composed of 11 components (excluding inevitable flavoring agents and preservatives) such as various plant extracts, various amino acids, various vitamin B and the like and is sugar-free. Through reasonable collocation of each component, the synergistic effect can be played, and the package with the total volume of 50 ml is adopted in the production, so that the carrying and the use are more convenient. The energy beverage provided by the invention has the main functions of eliminating fatigue and improving attention, has the health preserving and body building effects when being drunk frequently, and is an energy supplementing liquid. Experiments show that the energy beverage can achieve the aims of resisting fatigue and concentrating spirit. Specifically, in the present invention:

ginseng radix has effects in enhancing immunity, improving body constitution, improving intelligence, relieving depression, resisting aging, resisting inflammation and resisting oxidation^[3](L u, etc. 2009) Rg in ginseng has the functions of exciting central nerve, resisting fatigue, improving memory and learning ability and promoting synthesis of DNA and RNA, Rg1 in ginseng can quickly recover fatigue, improve learning memory, delay senility, has the functions of exciting central nerve and inhibiting platelet aggregation, Rb1 has the function of enhancing choline system, increasing synthesis and release of acetylcholine and improving memory, ginseng has the functions of improving attention and physical ability, and the addition of the element in the drink by the inventor can strengthen the body of a person who drinks the drink for a long time.

The medlar has the function of improving the anti-fatigue function of the organism and has obvious effect on the hypoxia tolerance^[4](An Wei et al 2011). The liver is the place where cholesterol and neutral fat are most synthesized. When people are tired, the liver function is also damaged, and the clinical manifestation is that the glutamic-pyruvic transaminase index in blood is increased. Organic germanium of fructus Lycii has effects of protecting liver, inhibiting accumulation of fat in liver, promoting activation of liver cell, and repairing damaged liver cell. In addition, the chemical components in the medlar have the function of removing free radicals of a human body, can effectively protect the endothelium of heart vessels, maintain the elasticity of the blood vessels, can also inhibit heartbeat, and is also helpful for adjusting arrhythmia. It also participates in vitamin A synthesis, and contains iron element for promoting hematopoiesis. In addition, vitamins B1, B2, C and calcium are beneficial to eye health.

The mint contains volatile oil, mint essence, tannin and other substances, is bitter in taste and cool in nature, is beneficial to calming anger, hysteria and fear, and has excellent curative effect on tired mood and depressed mood. Office workers working in front of computers for a long time should drink a cup of cool and refreshing mint tea when feeling poor spirit, which is beneficial to refreshing and restoring consciousness and relieving pressure.

The Korea fruit contains 2% of caffeine, fat, protein, etc., and has the same stimulating and exciting effects as tea and coffee, and Korea fruit powder as lipolysis promoter has effects of promoting microcirculation, relaxing nerve, and improving memory.

Taurine is an amino acid containing sulfur radical, is the most abundant amino acid in our body, and is mainly present in skeletal muscle, cardiac muscle, retina and brain tissue^[5](Imagawa et al 2009). Taurine is considered to be important for improving endurance and reducing lactic acid accumulation after exercise^[5](Imagawa et al 2009).

Tyrosine is an aromatic amino acid and is one of the 20 proteinogenic amino acids used to synthesize proteins, which is an optional amino acid, tyrosine is a very important component of body-building proteins and it also produces neurotransmitters such as levodopa, dopamine, noradrenaline and adrenaline, many dairy products, meat, fish, wheat, oats, and tyrosine-containing proteinaceous foods contain tyrosine L-tyrosine and helps to eliminate anxiety, depression, allergy, headache, and the like.

Phenylalanine is a nutritional supplement and one of the essential amino acids. Is converted into tyrosine in vivo, and promotes synthesis of thyroxine and kidney and adrenaline. Phenylalanine can reduce hunger; the sexual desire is improved; improving memory and improving agility of thinking; eliminating depression mood.

Niacin is a coenzyme for energy metabolism, fat synthesis and fat decomposition. The lack of nicotinic acid in the body can cause the symptoms of weight loss, fatigue, hypodynamia, poor memory, insomnia and the like. It can be used for relieving fatigue by supplementing nicotinic acid.

Folic acid, as a coenzyme of one-carbon-unit transferase system in biochemical reactions in vivo, acts as a one-carbon-unit transporter. Folic acid plays an important role in the synthesis of proteins and nucleic acids and the metabolism of various amino acids. Therefore, folic acid supplementation can increase physical performance and help alleviate fatigue.

Vitamin B6 is mainly in the form of pyridoxal 5-phosphate (P L P) as a coenzyme in transamination reactions, is also involved in α -amino acid decarboxylation and racemization and in lipid and nucleic acid metabolism, and is an essential coenzyme for glycogen phosphorylase.

Vitamin B12 is involved in folic acid metabolism and neurological function^[6](Wardlaw et al 2009). The vitamin B group such as vitamin B6, vitamin B12 and the like is a natural antidote for relieving pressure and nourishing nerves, is beneficial to quickly treating metabolites accumulated in a human body, is an essential nutrient for eliminating fatigue, is the most easily deficient vitamin, and is particularly beneficial to people with chronic fatigue syndrome by supplementing a proper amount.

The raw materials used in the invention are all nontoxic, and the dosage is also within the range permitted by human body. However, for safety, the inventors conducted safety tests on the PC12 cell line and Wister rat to verify the safety of the energy drink of the present invention.

Taking the following energy beverage as an example, the energy beverage is prepared from the following raw materials in 50 ml: total saponins of ginseng: 200 mg, medlar: 1 g (boiling in water for 30 minutes and removing residues for use), mint: 1 g (boiling in water for 30 minutes and removing residues for use), and kola fruit: 1 g (boiling in water for 30 minutes and removing residues for use), taurine: 1 g, tyrosine: 200 mg, phenylalanine: 200 mg, nicotinic acid: 30 mg, folic acid: 600 micrograms, vitamin B6: 6 mg and vitamin B12: 20 micrograms, add pure water to 50 ml.

1. PC12 adding cell culture solution of different concentrations of energy beverage

PC12 cells were cultured in a 10CM diameter petri dish, the bottom of which was treated with rat tail collagen, PC12 cells were cultured in 85% PRMI 1640 cell culture medium (GIBICO plus 5% calf serum, 10% horse serum, 50. mu.l/M L penicillin streptomycin at 7 concentrations of energy-containing beverages 1/10, 1/20, 1/40, 1/80, 1/160, 1/320, 1/640, respectively, for one week), and then observed under an inverted microscope, a fluorescence microscope, a scanning electron microscope, and a transmission electron microscope.

As a result: as shown in FIGS. 1 to 4, PC12 cells cultured in cell culture medium were added to "energy drink" at different concentrations, and the cells were observed with an inverted microscope after 7 days, and no morphological abnormality was observed. The fluorescence microscope is used for observing that the cell nucleus of PC12 cells cultured by different concentrations of energy beverage is intact without the phenomena of cell necrosis and apoptosis such as rupture, deformation and the like. The observation of a scanning electron microscope shows that the center of PC12 cells cultured by 'energy beverage' with different concentrations is full of full cell membranes, synapses grow vigorously and continuously, and no poor growth is seen. The observation of a transmission electron microscope shows that the cell nucleus is complete, the nuclear membrane is smooth, the shapes of mitochondria and endoplasmic reticulum are good, the cell plasma is rich, and the cell shape is not abnormal. In conclusion, PC12 cells did not show any abnormalities after 7 days of culture at different concentrations of "energy drink".

2. Safety test of "energy drink" fed to Wister rats

The method comprises dividing male Wister rats 16 with weight of 150 g into two groups, each group comprising 8 rats, wherein group 1 is a placebo group and is administered water with similar sense of 30M L every day, group 2 is fed with 30M L 'energy drink' every day for 3 months, and then killed by cervical dislocation method, immediately dissecting, and dissecting the chest and abdomen of the rats as shown in FIG. 5, showing the incision of the dissected rats.

As a result:

the Wister rats fed with 30 ml "energy drink" daily compared to the control group had no significant difference in animal body weight between the two groups after 3 months (p >0.05, student's t-test); gross anatomy showed that the "energy drink" group and the control group had no abnormality in hair, eyes, teeth, brain tissue, esophagus, stomach and intestine, liver and gall, heart, kidney, etc.

As shown in fig. 6, a schematic of the anatomical location of the cross-section of the brain. The experimental group and the control group have clear brain tissue gully, no pressure is applied to ventricles, and the brain tissue has no abnormalities such as edema and dehydration. As shown in fig. 7, HE staining at various levels of the brain showed that neurons in the brain were nuclear intact, full-fleshed plasma, intact vascular walls, no extravascular erythrocytes were free, glial cells were not edematous, and no inflammatory cell infiltration. There was no significant difference between the "energy drink" fed group and the control group. No obvious abnormality is found.

As shown in FIG. 8, the "HE" staining of the liver with "energy drink" showed smooth cell membranes, clear borders, no inflammatory cell infiltration, no fat deposition in hepatocytes, bile deposition and poor metabolism, which were normal hepatocytes. There was no significant difference from the control group.

As shown in fig. 9, staining of the kidneys with "energy drink" by "HE" showed smooth cell membranes, well-defined nuclei, clear borders, and no infiltration of red blood cells and inflammatory cells. Is a normal kidney, and has no obvious difference compared with a control group.

3. And (4) conclusion:

the above cell culture and animal experiments demonstrate that the "energy drink" of the invention is safe to use.

Compared with the prior art, the invention has the following advantages:

the invention relates to an energy beverage which is composed of 11 components (excluding inevitable flavoring agents and preservatives) such as various plant extracts, various amino acids, various vitamin B and the like and is sugar-free. By reasonably matching the components and playing a synergistic effect, the aims of resisting fatigue and concentrating spirit can be fulfilled. Experiments also show that the energy beverage is safe and effective, and the anti-fatigue effect can reach more than 4 hours.

Drawings

FIG. 1 is an inverted microscope image of PC12 cells cultured for 7 days with different concentrations of "energy drink".

FIG. 2 is a fluorescent microscope image of PC12 cells cultured with different concentrations of "energy drink" for 7 days.

FIG. 3 is a scanning electron microscope image of PC12 cells cultured with different concentrations of "energy drink" for 7 days.

FIG. 4 is a perspective electron micrograph of PC12 cells cultured for 7 days with different concentrations of "energy drink".

Fig. 5 is a schematic diagram of the anatomical route of the thoraco-abdominal portion of the rat.

Fig. 6 is a schematic diagram showing the position of 7 sections of brain tissue dissected after formalin fixation.

FIG. 7 is a graph showing HE staining of various layers of the brain with the "energy drink" of the invention. abcd represents microscopical observations of different parts of rat brain microscopically, a is from section 3; b from the 4 th section; c from the 5 th cut plane; d is from the 2 nd section.

FIG. 8 is a graphic representation of the "HE" staining of the liver with the "energy drink" of the present invention.

FIG. 9 is a graphic representation of the "HE" staining of the kidney with the "energy drink" of the present invention.

Fig. 10 is a schematic view of the seat and screen of a stim M300 type driving simulator.

Fig. 11 is a schematic view of a measurement method of SD L P (standard deviation of lateral position).

Fig. 12 is a graph of the results of comparing SD L P mean values between the "placebo" group, the "red ox" group, and the "energy drink" group.

Figure 13 is a graph of the results of a comparison of SDS mean values between the "placebo" group, the "red bull" group, and the "energy drink" group.

Figure 14 is a graph of the results of a comparison of the average KSS scores between the "placebo" group, the "red cattle" group and the "energy drink" group.

Detailed Description

The present invention will be described in more detail with reference to examples.

In the invention, all parts and percentages are weight units, and all equipment, raw materials and the like can be purchased from the market or are commonly used in the industry, if not specified. The methods in the following examples are conventional in the art unless otherwise specified.

Example 1

An energy beverage is prepared from the following raw materials in 50 ml:

total saponins of ginseng: 200 mg, medlar: 1 g, mint: 1 g, kola fruit: 1 g, taurine: 1 g, tyrosine: 200 mg, phenylalanine: 200 mg, nicotinic acid: 30 mg, folic acid: 600 micrograms, vitamin B6: 6 mg and vitamin B12: 20 micrograms of pure water is added to 50 milliliters,

the preparation process comprises the following steps: 1 g of each of medlar, mint and kola fruit is put into 40 ml of water to be boiled for 30 minutes, dregs are removed by filtration, and the ginseng total saponin: 200 mg, taurine: 1 g, tyrosine: 200 mg, phenylalanine: 200 mg, nicotinic acid: 30 mg, folic acid: 600 micrograms, vitamin B6: 6 mg and vitamin B12: 20 micrograms, add pure water to 50 ml. 50 ml of the prepared liquid was put into a glass bottle, the cap was screwed on, and the bottle was sterilized by heating in a water bath to 80 ℃ for 30 minutes.

Example 2

An energy beverage is prepared from the following raw materials in 50 ml:

total saponins of ginseng: 150 mg, wolfberry: 0.8 g, mint: 0.7 g, kola fruit: 0.8 g, taurine: 0.6 g, tyrosine: 160 mg, phenylalanine: 160 mg, niacin: 20 mg, folic acid: 400 micrograms, vitamin B6: 4 mg and vitamin B12: 18 micrograms, add pure water to 50 ml.

The preparation process comprises the following steps: putting 0.8 g of medlar, 0.7 g of mint and 0.8 g of kola fruit into 40 ml of water, boiling for 30 minutes, filtering to remove residues, adding the total saponins of ginseng: 150 mg, taurine: 0.6 g, tyrosine: 160 mg, phenylalanine: 160 mg, niacin: 20 mg, folic acid: 400 micrograms, vitamin B6: 4 mg and vitamin B12: 18 micrograms, add pure water to 50 ml. 50 ml of the prepared liquid was put into a glass bottle, the cap was screwed on, and the bottle was sterilized by heating in a water bath to 80 ℃ for 30 minutes.

Example 3

An energy beverage is prepared from the following raw materials in 50 ml:

total saponins of ginseng: 220 mg, medlar: 1.2 g, mint: 1.1 g, kola fruit: 1.2 g, taurine: 1.2 g, tyrosine: 210 mg, phenylalanine: 220 mg, niacin: 40 mg, folic acid: 700 micrograms, vitamin B6: 8 mg and vitamin B12: 22 micrograms, add pure water to 50 ml.

The preparation process comprises the following steps: putting 1.2 g of medlar, 1.1 g of mint and 1.2 g of kola fruit into 40 ml of water, boiling for 30 minutes, filtering to remove residues, adding the total saponins of ginseng: 220 mg, taurine: 1.2 g, tyrosine: 210 mg, phenylalanine: 220 mg, niacin: 40 mg, folic acid: 700 micrograms, vitamin B6: 8 mg and vitamin B12: 22 micrograms, add pure water to 50 ml. 50 ml of the prepared liquid was put into a glass bottle, the cap was screwed on, and the bottle was sterilized by heating in a water bath to 80 ℃ for 30 minutes.

Industrial applicability

To illustrate the efficacy of the "energy drink" of the present invention in resisting fatigue and increasing mental concentration, the following experiment was performed using the product prepared in any of the examples.

1. The experimental method comprises the following steps:

the applicant recruits 108 volunteers in the medical center of mississippi university by attaching advertisements to the campus, wherein the medical workers (28), the campus police (8) patrolling at night and the medical school students (72) with high learning pressure are included. The age is between 22 and 34 years. The tested person has a driving license for more than 2 years and drives more than 5000 kilometers per year. Normal body weight (21< BMI < 30; 55 kg to 85 kg). No bad taste such as smoke, wine, drugs, etc. The traditional Chinese medicine composition is not poor in sleep at ordinary times, is usually dozed in the daytime, and is removed from experimental subjects. In addition, exclusion conditions were more than 2 cups of coffee per day or energy drinks consumed within a week. Pregnant and lactating women were excluded. On the test day, coffee or other energy drinks could not be applied.

The entire experiment was completed in 4 days, and the test procedure was familiar to the subject on day 1. For the qualified testers, the STISI driving simulator is used for testing. After the simulated driving, the testee carries out the questionnaire survey of the simulated driving fatigue.

Subjects were randomized into 4 groups, group A with sugar water placebo, group B with 250M L "Red cattle", group C with 50M L "energy drink".

The eligible testers were run 4 times for 60 minutes each time. After each driving, a questionnaire was administered for 1 minute, including the quality of driving, the manner of driving, the degree of mental well-being, and the degree of drowsiness. The test was performed from 8 am to 1 pm.

TABLE 1 grouping test table

Placebo group	Red bull group	Energy beverage set
			Driving at 1 hour	Driving at 1 hour	Driving at 1 hour
Hour
	2 driving	Hour	2 driving	Hour	2 driving
Driving at 3 hours						Driving at 3 hours	Driving at 3 hours
	Driving at 4 hours	Driving at 4 hours	Driving at 4 hours

Long drive tests, as shown in fig. 10, simulators (model M300, system technology, inc., Hawthorne, CA, USA) were driven with stiim. The system comprises an adjustable seat, a driving test panel (instrument panel), a steering wheel, a clutch, an accelerator and a brake. At the same time, the sound system reflects the sound of the engine, brakes, turns, and leaves the road. When a car accident occurs, the visual and auditory effects of the windshield breakage will be simulated. The speed and gear are displayed on the dashboard.

The driving will simulate the driving of a 100 km highway, which is a bidirectional 4-lane highway with 3.5 m width each. The road condition simulates conventional one-way driving, and vehicles on the road have bridge beams, bends, go up small slopes and trees on the roadside. The speed of the tester is specified to be 95 km/h. This speed is controlled by the throttle and does not need to cruise. Mental concentration is required to bring the speed to exactly 95 km/h. If fatigue occurs, attention is not focused, the speed of a driver swings up and down, and the more fatigue, the larger the swing amplitude. The simulator will provide a parameter SDS (standard deviation of velocity) for the fatigue level of the test at the end of the test. This value will be used to evaluate the test results.

In addition, the tester requests to keep driving on the rightmost lane while keeping the speed of 95 km/h and keeping the vehicle running along the center of the right lane, and when the tester is tired, the speed cannot be kept constant on the one hand and cannot be kept running on the center of the road on the second hand, thus generating a measurement method of a parameter SD L P (standard deviation of lateral position). SD L P (standard deviation of lateral position) as shown in fig. 11.

The larger the SD L P value of the tested person is, the more the thought can not be concentrated on the road surface, which is a sensitive index for testing fatigue, some domestic SD L P is used as the judgment standard for drunk driving^[7](L outseens et al 1987) but when a preceding vehicle is encountered that is traveling too slowly and requires overtaking, the SD L P value is not counted during overtaking.

Questionnaire, after driving for 1 hour, the subject answered the questions on the KSS (Karolinska sleepiness Scale) questionnaire for 1 minute^[8](Akerstedt et al 1990). The following are KSS scoring criteria: 1 point (very awake); 3 minutes (awake); 5 points (sleepy but not difficult to keep awake); 7 points (very sleepy); to 9 points (sleepiness, struggle with drowsiness). And continuing driving after the question is asked.

SD L P, SDs, and KSS values were statistically analyzed using SAS statistical software, ANOVA, (bi-directional, P < ═ 0.05).

2. As a result:

108 volunteers participated in the study. 3 male volunteers and 1 female volunteers were deleted because they did not comply with the experimental conditions. Data were analyzed for a total of 104 volunteers. Of which 62 men and 42 women. Age 23.2 ± 1.5 years; the normal BMI of the system is 22.8 plus or minus 1.8; the time for owning the driving license is 47.2 +/-15.6 months; the mileage of the vehicle is 10,785 +/-5021 kilometers in each year; sleep quality was normal 1 day before the test.

TABLE 2

Table 2. stim driving simulator for placebo, "red cattle" and "energy drink" groups showed statistical analysis of ANOVA for SD L P, SDs parameters (mean SD), and KSS questionnaire score (mean SD) (. denotes P <0.05 compared to placebo).

The comparison of the SD L P mean values between the groups is shown in fig. 12, the SD L P values for each group tended to increase over time, it can be seen that the "red cattle" group and the placebo group did not differ much-the "energy drink" group SD L P mean values were significantly less (P <0.05) than the placebo group, indicating that the subjects taking the "energy drink" had significant concentration, this effect lasted for more than 4 hours-the data for the "energy drink" compared to "red cattle", the "energy drink" was significantly better effective than "red cattle" (P < 0.05).

The results of the comparison of the SDS mean values between the respective groups are shown in fig. 13. The SDS values tended to increase with time for each group. It can be seen that the group taking "red cattle" and the placebo group did not differ much. The "energy drink" group SDS mean was significantly smaller than the placebo group (p < 0.05). This means that the attention of the tested person taking the "energy drink" was clearly focused and the effect lasted for more than 4 hours. Compared with the data of the 'energy beverage' and the 'red cattle', the 'energy beverage' has obviously better effect than the 'red cattle' (p < 0.05).

The results of the comparison of the average KSS scores between the respective groups are shown in fig. 14. The KSS values were significantly lower (p <0.05) for the "energy drink group" compared to the placebo group at each time period. It is shown that energy drinks are effective against fatigue. The KSS value of the red cattle group at 1 hour is obviously superior to that of the placebo group (p <0.05), which indicates that the anti-fatigue effect of the red cattle group at 1 hour is certain; however, the KSS values at hours 2, 3 and 4 were not significantly effective compared to the placebo group. The KSS values of the "energy drink" group and the "red cattle" group were not much different at 1 hour, but were significantly better at 2, 3, and 4 hours than those of the "red cattle" group. The effect of the energy beverage is obviously longer than that of the red cattle.

3. And (4) conclusion:

from the above experiments, it can be seen that the "energy drink" of the present invention has a significant effect (P <0.05) in each period in detecting 3 indicators of fatigue (SD L P, SDS, KSS score) compared to the placebo group.

In this trial, only at hour 1, the KSS score was significantly better than that of the placebo group (p < 0.05). The red cattle has certain effect on resisting fatigue, but the duration is not long. This experiment is similar to our usual experience, and we feel that "red cattle" has some anti-fatigue effect, but not very obvious. The KSS value of the real overcoming of the difficulty is more than 7 points, the red cattle is used for overcoming the difficulty, and the possibility of continuing working is low.

Reference documents:

1.Van Laar W,Simpson H,Mayhew D,Robertson R(2008)Fatigued and drowsydriving:a survey of attitudes,opinions and behaviors.J Safety Res 39:303-309.

2.Maycock G(1996)Sleepiness and driving:the experience of UK cardrivers.J Sleep Res 5:229-37.

3.Lu JM,Yao Q,Chen C.2009.Ginseng compounds:an update on theirmolecular mechanisms and medical applications.Cur Vascul Pharmacol 7:293-302.

4. anwei, Wangyaman, Shizhigang 1, Zhaojianhua 1, Wangxiao, Lihong soldier influence of matrimony vine on anoxia resistance and antifatigue ability of mice Jiangsu: jiangsu agricultural science, 2011: ,2011,39(5):396-397

5.Imagawa TF,Hirano I,Utsuki K,Horie M,Naka A,Matsumoto K,ImagawaS.2009.Improved cycling time-trial performance after ingestion of a caffeineenergy drink.Int J Sport Nutr Exerc Metab 19:61-78.

6.Wardlaw GM,Smith AM.2009.Contemporary nutrition a functionalapproach.New York:McGraw-Hill.

7.Louwerens JW,Gloerich ABM,De Vries G,Brrokhuis KA,O’Hanlon JF.(1987)The relationship between drivers’blood alcohol concentration(BAC)andactual driving performance during high speed travel.In:Noordzij PC,Roszbach R(eds)Alcohol,Drugs and traffic safety.Excepta Medica,Amsterdam,pp 183-192.

8.Akerstedt T,Gillberg M(1990)Subjective and objective sleepiness inthe active individual.Int J Neurosci 52:29-37。

Claims (4)

1. An energy beverage is characterized in that the energy beverage is prepared from the following raw materials in 50 ml:

total saponins of ginseng: 150-220 mg, wolfberry: 0.8-1.2 g, mint: 0.7-1.1 g, kola fruit: 0.8-1.2 g, taurine: 0.6-1.2 g, tyrosine: 160-210 mg, phenylalanine: 160-220 mg, niacin: 20-40 mg, folic acid: 400-700 micrograms, vitamin B6: 4-8 mg and vitamin B12: 18-22 micrograms, adding pure water to 50 ml.

2. The energy beverage of claim 1, wherein said wolfberry is added in the form of a plant extract.

3. The energy beverage of claim 1, wherein said mint is added in the form of a plant extract.

4. The energy beverage of claim 1, wherein said kola nut is added in the form of a plant extract.

Images