CN106721074A - The method for being combined production specific yolk antibody preparation with the specific egg pulp that shells using the zeolite powder of ultramicro grinding - Google Patents
The method for being combined production specific yolk antibody preparation with the specific egg pulp that shells using the zeolite powder of ultramicro grinding Download PDFInfo
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- CN106721074A CN106721074A CN201611185389.7A CN201611185389A CN106721074A CN 106721074 A CN106721074 A CN 106721074A CN 201611185389 A CN201611185389 A CN 201611185389A CN 106721074 A CN106721074 A CN 106721074A
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- ultramicro grinding
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Abstract
The method for being combined production specific yolk antibody preparation with the specific egg pulp that shells using the zeolite powder of ultramicro grinding, by mass percentage, the specific yolk antibody preparation is made up of following components：Specificity is shelled egg pulp 20% 80%, the zeolite powder carrier after ultramicro grinding 20% 80%, potassium sorbate 0.05% 0.2%, calcium propionate 0.1% 0.5%.Product of the invention is suitable to the animals such as direct feeding stichopus japonicus, turbot, milk cow and piglet, can be used to prevent and treat bacterium infection or other immunological diseases, greatly improves the survival rate and immunity of animal.In addition, the production technology of product of the present invention is greatly simplified, production cost is greatly lowered, and is more suitable for large-scale production.
Description
Technical field
The invention belongs to biological feedstuff technical field, it is related to a kind of special yolk for Animal diseases prevention and treatment
Antibody preparation is formulated and preparation method.
Background technology
Due to medicament residue caused by the use of antibiotic, it has also become the aquatic products product competitiveness in the international market of restriction China
A subject matter.IgY (Yolk antibody) has the advantages that nontoxic, noresidue, does not develop immunity to drugs, and development prospect is enjoyed looks steadily
Mesh.In addition IgY preparation costs are low, output capacity is high, purification is simple and efficient, and have the advantages that heat-resisting, good stability, wide
Product is made generally to carry out the prevention and treatment of Animal diseases.For example sea cucumber is prevented and treated with the IgY preparations being made come feeding sea cucumber
Skin ulcer syndrome etc..
Current IgY products are mostly freeze-dried powder or sheet, ointment preparation, but freeze-dried powder and sheet, ointment preparation
Preparation technology it is complicated, it is relatively costly, and active ingredient has greater loss during purification is prepared.Examined based on more than
Consider, we are directly combined the specificity egg pulp that shells containing special yolk antibody with the zeolite powder of ultramicro grinding, are made feeding
Feed additives, not only solve the problems, such as loss of effective components but also preparation technology are greatly simplified, save mass energy, are produced into
Originally it is greatly lowered.
The content of the invention
Inventor has thought better of the purification preparation method of characteristic Yolk antibody from cost-effective, and therefrom inhales
The experience of preciousness is taken, the method directly mixed using the zeolite powder and the specific egg pulp that shells of ultramicro grinding makes product not only
Functionally fully meet requirement, and save the big energy that consumption is needed in Yolk antibody purification process, reduce life
Produce cost.
A kind of specific yolk antibody preparation of the present invention, according to mass ratio, is made up of following components：
In ultramicro grinding, the auxiliary agent for using is sodium carbonate or sodium acid carbonate, and auxiliary dosage is 1%-8% (w/w), ultra micro
Grinding time is 3min-15min.
The shell quality proportioning of egg pulp and ultramicro grinding zeolite powder of specificity is 1:4-3:1.
Described specific yolk antibody preparation preparation method, comprises the following steps：
(1) by after zeolite powder powder ultramicro grinding, 60-200 mesh sieve is crossed standby；
(2) high-immunity egg is shelled and is squeezed into container, stirred；
(3) the specificity egg pulp that shells is stirred to volumetric expansion, lighter, in thick；
(4) addition potassium sorbate, calcium propionate, stir；
(5) add specificity to shell in egg pulp the zeolite powder carrier after sieving, stir, formed neither too hard, nor too soft
Bulk solid；
(6) soft particle is made of granulator；
(7) dry at room temperature；
(8) separating twice sieving；
(9) it is made feed addictive.
Compared with prior art, the beneficial effects of the invention are as follows：
The present invention plays the purpose of optimum efficiency and energy saving reduces cost from IgY, changes traditional system
The method of standby IgY products, is added in the middle of feed after IgY no longer is made into freeze-dried powder or purification as feed addictive.
But will directly contain the shell zeolite powder of egg pulp and ultramicro grinding of the specificity rich in specific IgY carries out hybrid process,
So not only reduce separating, in purification and freeze-drying process IgY loss, and also simplify production technology, this product
It is important that being exactly, natural zeolite powder carrier needs a large amount of consumption in solving conventional Yolk antibody production purification process
The problem of the energy, greatly reduces cost.
The various methods of table 1 produce IgY preparation Integrated comparatives
As it can be seen from table 1 in the case where the factors such as cost of labor and equipment depreciation are not considered, this method early investment
Equipment cost it is minimum, it is even more many that specific energy consumption falls below original 1/5, and significantly, and production process is simple for energy-saving effect,
Flexibly, restraining factors are less, with very strong actual application value for the mode of production.
Brief description of the drawings：
Fig. 1 is the flow chart of low cost production specific yolk antibody preparation；
Fig. 2 is common zeolite powder stereoscan photograph；
Fig. 3 is ultramicro grinding zeolite powder stereoscan photograph；
Fig. 4 is standard IgY curves；
Fig. 5 is the light absorption value of different times IgY preparations.
Specific embodiment
Describe specific embodiment of the invention in detail below in conjunction with technical scheme.
Embodiment 1
Zeolite powder meal is carried out into ultramicro grinding, auxiliary agent is added during ultramicro grinding, the auxiliary agent for using is sodium carbonate, auxiliary agent is used
Measure is 5% (w/w), grinding time 10min.
Contrasted by stereoscan photograph, it can be found that zeolite powder is by after ultramicro grinding, its surface pore is more,
Surface area becomes much larger, and it is stronger that corresponding its combines adsorption capacity.Experiment shows, relative to common zeolite powder, using Ultramicro-powder
Zeolite powder carrier adsorption capacity after broken treatment can increase 30%-90%.
Embodiment 2
By zeolite powder by after ultramicro grinding treatment, crossing 100 mesh sieve, 300g is weighed standby；Will be yellow by injection inactivation
High-immunity egg after extra large Shewanella is immune, shells, and takes 200g and squeezes into container, stirs；Egg yolk liquid is stirred to volumetric expansion, face
Discoloration is shallow, in thick；Addition 0.5g potassium sorbates, 1.5g calcium propionates, stir；Zeolite powder after sieving is added to spy
The opposite sex is shelled in egg pulp, is stirred until homogeneous, and forms the moderate lumps solid of soft durometer；Then it is made diameter with granulator
The soft particles of 3mm；Dry at room temperature；100 mesh sieve, packaging are crossed after being crushed with pulverizer；It is made feed addictive.
Embodiment 3
By zeolite powder by after ultramicro grinding treatment, crossing 60 mesh sieve, 500g is weighed standby；Will be magnificent by injection inactivation
High-immunity egg after vibrios is immune, shells, and takes 350g and squeezes into container, stirs；The egg pulp that will shell is stirred to volumetric expansion, color
Shoal, in thick；Addition 1.5g potassium sorbates, 4.5g calcium propionates, stir；Zeolite powder after sieving is added to and is shelled
In egg pulp, it is stirred until homogeneous, forms the moderate lumps solid of soft durometer；Then the soft particles of diameter 3mm are made of granulator；
Dry at room temperature；100 mesh sieve, packaging are crossed after being crushed with pulverizer；It is made feed addictive.
Embodiment 4
IgY content detections
Obtained IgY preparations 1g, plus 30ml coating buffers are weighed, ultrasonic oscillation 15min after 100 times of dilution, adds per hole
100ul is coated with 96 hole elisa Plates, and 4 DEG C overnight.ELISA Plate is washed with cleaning solution 3 times, 100ul confining liquids, 37 DEG C of incubations are added per hole
Dried after 2h, washed 3 times, then add rabbit-anti chicken IgG-HRP100ul, 37 DEG C of reaction lh to each hole；Cleaning solution washs each hole 5 times, often
Hole adds 100ul tmb substrate buffer solutions, and develop the color 20min at room temperature, and 50ul 2M sulfuric acid terminating reactions are added per hole, is surveyed with ELIASA
OD450/630nm.Then with standard IgY curve comparisons, the specific IgY content in different times sample is calculated.
As seen from Figure 4, this product has no in half a year internal specific IgY content and substantially reduces and maintain shape higher
State, illustrate this product can in longer time continuous and effective.
Claims (4)
1. production specific yolk antibody preparation, its feature are combined using zeolite powder and the specificity of the ultramicro grinding egg pulp that shells
It is that each component is configured by following mass percent：
2. the zeolite powder of utilization ultramicro grinding according to claim 1 and the specificity egg pulp that shells are combined production specificity
Yolk antibody preparation, it is characterised in that described ultramicro grinding zeolite powder in ultramicro grinding, the auxiliary agent for using be sodium carbonate or
Sodium acid carbonate, auxiliary dosage is 1%-8% (w/w), and the ultramicro grinding time is 3min-15min.
3. the zeolite powder of utilization ultramicro grinding according to claim 1 and the specificity egg pulp that shells are combined production specificity
Yolk antibody preparation, it is characterised in that the shell quality proportioning of egg pulp and ultramicro grinding zeolite powder of specificity is 1:4-3:1.
4. a kind of preparation method of specific yolk antibody preparation described in claim 1, it is characterised in that comprise the following steps：
(1) by after zeolite powder powder ultramicro grinding, 60-200 mesh sieve is crossed standby；
(2) high-immunity egg is shelled and is squeezed into container, stirred；
(3) the specificity egg pulp that shells is stirred to volumetric expansion, lighter, in thick；
(4) addition potassium sorbate, calcium propionate, stir；
(5) add specificity to shell in egg pulp the zeolite powder carrier after sieving, stir, form neither too hard, nor too soft bulk
Solid；
(6) soft particle is made of granulator；
(7) dry at room temperature；
(8) separating twice sieving；
(9) it is made feed addictive.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611185389.7A CN106721074A (en) | 2016-12-20 | 2016-12-20 | The method for being combined production specific yolk antibody preparation with the specific egg pulp that shells using the zeolite powder of ultramicro grinding |
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|---|---|---|---|
| CN201611185389.7A CN106721074A (en) | 2016-12-20 | 2016-12-20 | The method for being combined production specific yolk antibody preparation with the specific egg pulp that shells using the zeolite powder of ultramicro grinding |
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Citations (6)
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|---|---|---|---|---|
| CN102308914A (en) * | 2011-07-25 | 2012-01-11 | 大连赛姆生物工程技术有限公司 | Animal dietotherapy IgY whole egg pulp paste preparation and its preparation method |
| CN102429097A (en) * | 2011-07-25 | 2012-05-02 | 大连赛姆生物工程技术有限公司 | Food therapy yolk antibody yolk liquid pasty preparation for animals and production method thereof |
| CN102696922A (en) * | 2012-06-14 | 2012-10-03 | 昆山贝瑞康生物科技有限公司 | Intestine protecting compound for sea cucumbers and preparation thereof |
| CN103416825A (en) * | 2012-05-18 | 2013-12-04 | 辽宁省畜牧科学研究院 | Granulating-dedicated coarse feed composite mildew inhibitor and preparation method thereof |
| CN105198988A (en) * | 2015-09-30 | 2015-12-30 | 大连理工大学 | Vibrio-splendidus-resistant egg yolk antibody and preparation method thereof |
| CN105211501A (en) * | 2015-11-02 | 2016-01-06 | 山东贝瑞康生物科技有限公司 | A kind of health-care agent improving fur-bearing animal anti-stress ability |
-
2016
- 2016-12-20 CN CN201611185389.7A patent/CN106721074A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102308914A (en) * | 2011-07-25 | 2012-01-11 | 大连赛姆生物工程技术有限公司 | Animal dietotherapy IgY whole egg pulp paste preparation and its preparation method |
| CN102429097A (en) * | 2011-07-25 | 2012-05-02 | 大连赛姆生物工程技术有限公司 | Food therapy yolk antibody yolk liquid pasty preparation for animals and production method thereof |
| CN103416825A (en) * | 2012-05-18 | 2013-12-04 | 辽宁省畜牧科学研究院 | Granulating-dedicated coarse feed composite mildew inhibitor and preparation method thereof |
| CN102696922A (en) * | 2012-06-14 | 2012-10-03 | 昆山贝瑞康生物科技有限公司 | Intestine protecting compound for sea cucumbers and preparation thereof |
| CN105198988A (en) * | 2015-09-30 | 2015-12-30 | 大连理工大学 | Vibrio-splendidus-resistant egg yolk antibody and preparation method thereof |
| CN105211501A (en) * | 2015-11-02 | 2016-01-06 | 山东贝瑞康生物科技有限公司 | A kind of health-care agent improving fur-bearing animal anti-stress ability |
Non-Patent Citations (2)
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| 赵余庆: "《中药及天然产物提取制备关键技术》", 31 January 2012, 中国医药科技出版社 * |
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Application publication date: 20170531 |