CN106720660A - A kind of Lingshan snake gallbladder tea and preparation method thereof - Google Patents
A kind of Lingshan snake gallbladder tea and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of Lingshan snake gallbladder tea and preparation method thereof, comprise the following steps：1) snake gall source is chosen：From pollution-free foster She Chang, propagate the snake of time, healthy strong high-quality artificially；2) snake gall is extracted：Snake gall is taken out after killing snake, blood stains are cleaned, embathed in wine, placed in a reservoir, soaked with wine, it is stand-by；3) snake gall treatment：1. by step 2) soaked snake gall taking-up, puncture, extrude bile, filtering；2. 1. obtain adding pure water in bile to step, be well mixed；3. the snake gall water for 2. being obtained to step with sealing high-temperature steam carries out sterilization, is subsequently adding high pressure homogenizer homogeneous；4. the cobra 3. processed through step, mucosal rat snake snake gall water are well mixed, are configured to snake gall mother liquor standby；5. the snake gall mother liquor pure water dilution for 4. step being obtained, it is standby；4) snake gall liquid mixes with sample tea：By step 3) the snake gall liquid that obtains, it is vaporific to be sprayed in sample tea, stir and allow it to fully absorb, bakee snake gallbladder tea.
Description
Technical field
The present invention relates to Tea Processing technical field, specifically a kind of Lingshan snake gallbladder tea and preparation method thereof.
Background technology
Snake gall (Snake gall), is the gall-bladder of storage bile in snake body.Snake gall is cold in nature, bitter but slightly sweet taste；Dispelled with wind-dispelling
Effect of miliaria is gone in dehumidifying, refrigerant improving eyesight, removing toxic substances；The adjustable nervous system for mending people, internal system and immune system, delay body
Aging.All of snake gall can all be used as medicine, particularly the courage of banked krait, Bungarus multicinctus, cobra, king cobra, long-noded pit viper, pallas pit viper,
The valable starting materials being even more used as medicine.But because that may contain parasite in raw snake gall, people easily causes physical impairment after consumption, if
Take, need to be by snake gall steamed wheaten foods.
Snake gall is exactly since ancient times a kind of rare medicinal herbs, is widely used in clinical and among the people.Record at first and see Han dynasty《Name
Doctor does not record》, there is two kinds of boa (boa) courage and brevicaude pit viper gall.Snake gall is cold in nature, bitter but slightly sweet taste, has promoting the circulation of qi eliminating the phlegm, searches wind clearing damp, improving eyesight
Effect of beneficial liver.There is good result to diseases such as cough ant phlegm, red eye, swell pain, neurasthenia, unconsciousness due to high fever, child convulsions.According to
Clinical report, snake bile is used to treat various ulcers of the cornea, superficial punctate keratitis, shallow-layer diffusivity keratitis, aglia etc.
Disease has preferable curative effect, and has no side effect.In addition, snake gall application is convenient, can both be swallowed with boiling water, it is also possible to taken with yellow rice wine.
Additionally, by the viewpoint of the traditional Chinese medical science, it searches that wind position is different for different snake gall, and synergy can receive more preferable curative effect.Such as Ptyas korras
Courage can search top wind, and cobral gall can search the wind at middle part, and banded krait gall can search the wind of bottom.Cobral gall be it is traditional it is rare in
Medicinal material, it is among the people be also to enjoy high praise.Meanwhile, modern medicine research shows：The medicinal effects of snake gall are mainly bile acid, but still
There are other many curative effects with anti-inflammation, it is seen that also contain other effective ingredients in snake gall.Bile is removed in the bile of snake gall
It is sour outer, also rich in trace element and Cu, Fe and Ca, Mg etc..Trace element to the active function of various enzymes in human body, to nucleic acid,
The synthesis of protein, immunologic process, cell proliferation have a significant impact, can adjust comprehensively mend people nervous system, internal system and
Immune system, promotes the reparation of epithelial cell, increases cell division, increased activity, so as to strengthen immunologic function to organism,
In safeguarding people's hormone in vivo and delaying the vital movements such as senility of humanbody, also play an important role.Snake gall is often taken, can be passed through
Human body in adjustment human body, improves human body circulation, and strengthen immunity reaches the purpose effect controlled in outer disease extremely good.
Snake gall clinical practice is quite extensive, and existing more than 2,000 years history, its medical value are it is known that undisputed.
But the source of its snake gall used, processing mode, application method and consumption have strict regulation, especially needed using wild snake gall
Sincerely to use.Because present wild snake one is living environment pollution from easily infection parasite, two is that it is a kind of violent climbing
Action thing, is food with mouse, the frog, bird etc., often infects the parasitic disease of some Zoonosis, such as porocephaliasis.Other wild snake
Class lists state guarantee animal in, for the health of itself, wild animal is not eaten excessively, and this is also one of performance of human civilization.
Tealeaves (tea leaves), is one of famous big beverage in the world three, is referred to as " emperor of east beverage ".Tealeaves
Peculiar beverage as China is with a history of thousands of years.Successive dynasties " book on Chinese herbal medicine " class medical book says that it has only when tealeaves is referred to
Yearningly, clear god, diuresis, control cough, eliminating the phlegm, improving eyesight, beneficial thinks of, relieving restlessness eliminating greasiness, drive be stranded make light of one's life by commiting suicide, eliminating inflammation and expelling toxin the effects such as.Through analysis, tealeaves
In containing caffeine, tannin, Tea Polyphenols, protein, carbohydrate, free amino acid, chlorophyll, carrotene, aromatic oil,
The Multiple components of enzyme, provitamin A, vitamin B, vitamin C, vitamin E, citrin and inorganic salts, trace element etc. 400.
Modern medicine study shows that digestive pharmacological action of drinking tea is chiefly to facilitate the metabolism of body fat and improves gastric juice
And the secretory volume of other digestive juices, promote digesting and assimilating for food.Often drinking tea to enhance metabolism, and enhancing cardiac function is also
Blood pressure is advantageously reduced, artery sclerosis is prevented, the effects such as having prevention and suppress fat.There are six big tea systems, kind and processing in China
Technique is different, function also difference.Tealeaves how is allowed to play maximum effect, it is also one that selection is adapted to the tealeaves of oneself
Knowledge, " different tea, the method preserve, brew, drunk all respectively is worthy of careful study.
Green tea, is unleavened, and at most, it is Tang Qingye to drink most commonly used plant tea its features to Chinese yield
It is green.Effect of green tea and effect：Modern science numerous studies confirm that green tea contains the biochemistry closely related with health really
Composition, not only clear away heart-fire with refreshing oneself, clearing away summerheat, eliminate indigestion and phlegm, eliminating greasiness fat-reducing, relieving restlessness that clears away heart-fire, removing toxic substances are sobered up, promote the production of body fluid to quench thirst,
The pharmacological actions such as fall fire improving eyesight, stop dysentery dehumidifying, also to modern diseases, such as Radiation sickness, cardiovascular and cerebrovascular diseases, cancer disease have one
Fixed pharmacological effect.
Iron Guanyin, unique " kwan-yin rhythm ", the refined rhythm of delicate fragrance, " seven bubble lingering fragrance small streams month reveal Xi Le ridges clouds with joy ".Except with one
As tealeaves healthcare function outside, also with anti-aging, anticancer, anti arteriosclerosis, prevent and treat diabetes, defatting beauty, preventing and treating dental caries
Tooth, heat-clearing and fire-reducing, oppose (tea rivers and lakes) the effects such as cigarette is sobered up.Through scientific analysis and it was verified that Iron Guanyin contains amino higher
Acid, vitamin, mineral matter, Tea Polyphenols and alkaloid, there is various nutrition and effective component, and with the improving eyesight that clears away heart-fire, bactericidal antiphlogistic subtracts
The effects such as fertile appearance and anti-aging, anti-cancer, the blood fat that disappears, reduction cholesterol, reduction angiocardiopathy and diabetes.
Black tea, there occurs the chemical reaction centered on Tea Polyphenols enzymatic oxidation in process, the chemistry in fresh leaf
Composition transfer is larger, and Tea Polyphenols reduces more than 90%, generates the new components such as theaflavin, thearubigin.Aroma substance is brighter than fresh leaf
It is aobvious to increase.So black tea has the feature of black tea, red soup, red autumnal leaves and fragrant and sweet taste alcohol.Black tea there are the whole intestines of preventing decayed tooth, stomach invigorating to help disappear
The effects such as change, delaying aging, hypoglycemic, lowering blood pressure and blood fat, anticancer, radioresistance.It has often been said that, black tea, man are drunk woman more
Drink green tea more, often drink black tea and can be good at the effect such as cardioprotection, anticancer, anti-aging.
Guangxi Lingshan County Tea Production is with a long history, and according to load, the Yuan Dynasty starts plantation, and large area plantation also has more than 500 years,
Guangxi 25 years (1899)《West of Guangdong Province Yuexi small stream overflows trivial note》The double phoenix tea in the upper record Lingshan are Zhuang rural area tea well-known in the localities.It is existing
It is " township of Chinese famous tea ", " national emphasis Chan Cha counties ", " national ten big ecology Chan Cha counties " in, Lingshan County.Whole county tea in 2014
6.46 ten thousand mu of leaf cultivated area, obtains the non-polluted farm product place of production and assert certificate (5.57 ten thousand mu of certification area), and certification area is accounted for
Whole county tealeaves area 90.13%., 7494 tons of tea yield, 310,410,000 yuan of the output value.Tea tree principal item is good fortune cloud six, the Lingshan
Middle period kind, big-leaf species in yunnan；The kinds more than 30 such as Longjing Changye, golden kwan-yin, Iron Guanyin.The tealeaves class of processing has green tea (silver-colored spiral shell, hair
Peak, pine needle, Longjing tea etc.), black tea (congou tea), Iron Guanyin etc., based on green tea.
Guangxi Lingshan County, 21.7 DEG C of whole county average temperature of the whole year, -0.8 DEG C of extremely low temperature, the very suitable snake of superior climatic environment
Class breeds growth.Lingshan peasant make use of this geographical and climatic superiority, by for many years to study intensively foster snake backward in technique, 1998
Year and the cultural technique for having grasped mucosal rat snake and cobra for 2000 respectively.During " 12 ", the Lingshan is supported snake industry and is rapidly sent out
Exhibition.Support snake family and develop into 6800 families from family more than 100, cultivate cobra and mucosal rat snake 10,020,000, it is average annual to cultivate 200.4 ten thousand,
21.34 hundred million yuan are taken in, average annual 4.26 hundred million yuan of income.The whole county has peasant family more than 2000 to shift to an earlier date 8 years and enjoy a fairly comfortable life life by supporting snake,
There is 4500 families people more than 30,000 to shake off poverty and set out on the road to prosperity by supporting snake, accounting for the whole county same period shakes off poverty more than the 30% of number, and supporting snake industry not only turns into the Lingshan
Industry of getting rich, and as during important anti-poverty project " 12 ", snake industry development is supported in Lingshan County, causes a sensation throughout the country, and shakes
Shake the world.
Snake is supported in China by the world, and the foster snake of China sees Guangxi, the Yang Shekan Lingshan, Guangxi.Lingshan County is unique " foster snake of China
Township ", snake drug enriches, its middle age cultivation cobra more than 1,000,000.The mucosal rat snake of Lingshan County cultivation and cobra, category
In national " three have " protection animal, animal is laid special stress on protecting in Guangxi, because processing industry is not matched, the mucosal rat snake of current Lingshan cultivation and
Cobra (fresh and alive snake) primarily enters the emerging rich market sale of Conghua of Guangdong Province, and whole body is all that precious snake is not fully used, and is produced
Industry added value is not high.That is, the internal organ such as more than sold in year 100 ten thousand snake venom, snake gall, snake oils of cobras cannot profit
With having wasted the precious resources of cobra.
The content of the invention
It is an object of the invention to cobra, mucosal rat snake bile using Lingshan cultivation, with Lingshan high-quality tea through science
Proportioning, strict processing, is refined into healthy and beneficial Lingshan snake gallbladder tea worthy of the name.
A kind of preparation method of Lingshan snake gallbladder tea, comprises the following steps：
1) snake gall source is chosen：From pollution-free foster She Chang, propagate the snake of time, healthy strong high-quality artificially；
2) snake gall is extracted：Snake gall is taken out after killing snake, blood stains are washed, embathed in wine, be placed in getting container ready, used
Wine soaks, stand-by；
3) snake gall treatment：
1. by step 2) soaked snake gall taking-up, puncture, extrude bile, filtering；
2. 1. obtain adding pure water in bile to step, be well mixed；
3. the snake gall water for 2. being obtained to step with sealing high-temperature steam carries out sterilization, is subsequently adding high pressure homogenizer homogeneous；
4. the cobra 3. processed through step, mucosal rat snake snake gall water are well mixed, are configured to snake gall mother liquor standby；
5. the snake gall mother liquor pure water dilution for 4. step being obtained, it is standby；
4) snake gall liquid mixes with sample tea：By step 3) the snake gall liquid that obtains, it is vaporific to be sprayed in sample tea, stir and allow it
Fully absorb, bakee snake gallbladder tea.
Step 1 of the present invention) described in snake, preferably propagate artificially more than 12 months time, 2 kilograms of body weight, health it is strong excellent
Matter, the snake without parasite, cultivar origin is in cobra, mucosal rat snake.
Step 2) described in extraction snake gall, take out snake gall after preferably killing snake, blood stains are washed, with more than 60 degree of pure rice
Steeping in wine is washed 5 minutes, is placed in getting glass container ready, is soaked 1 month with more than 60 degree of pure rice wines, stand-by.
Step 3) 2. in, it is described 1. to obtain adding pure water in bile to step, preferably according to 1:20 weight ratio；
The 3. described sterilization of step, preferably 160 DEG C of steam treatment 20 minutes, not existing by the snake gall of high temperature steam treatment has parasitism
The danger of worm, preferably described high pressure homogenizer homogeneous, 10000psi, 5min；Step 4. described in cobra, slide-mouse calmy
Courage water is well mixed, preferably according to 3:1 weight ratio；The 5. described snake gall mother liquor pure water for 4. obtaining step of step is dilute
Release, preferably according to 1:20 weight ratio.
Step 4) described in fully absorb, preferably 3-4 hours；Described step 3) the snake gall liquid that obtains and sample tea, it is excellent
Choosing is according to 1:100 weight ratio, in the non-harmful produced black tea of described sample tea, preferably high-quality, green tea or extra-strong tea
One kind.
Present invention additionally comprises the snake gallbladder tea obtained using method made above, according to the principle of " integration of drinking and medicinal herbs ", using snake
Courage and tealeaves effect and effect, science are made, and both pass on traditional Chinese tea culture, and the healthy of compatriots is beneficial to again,
Make main flow health " national drink " of " liquid of health, the drink of soul ".
Compared with prior art, the present invention has advantages below:
1st, at present, domestic also several enterprises' production snake gallbladder teas, but it is reported that majority is merely nominal, one is to utilize people
Extensive cognition to snake gall and tealeaves pharmacological effect, takes its name, the health protection tea really based on Chinese herbal medicine；Another kind is certain
With tealeaves, but snake gall deal very pettiness, there is no snake gall effect.In present in the market, without using cobra, shuilu snake gall
Direct and tealeaves the collocation of juice.Snake gall is strictly a herb, cold in nature, bitter but slightly sweet taste, with work(such as the refrigerant improving eyesight of dispelling wind and eliminating dampness
Effect, but effect is not reached without certain deal, this consumption of the invention is the optimal knot for drawing by hundred test of many times
Really.
2nd, the medical value and edibility of snake gall are completely different, and source, processing mode of medicinal snake gall etc. have strictly
Regulation, process and harmful poisonous material can be removed in processing procedure, and life cut open now gulp down and dining table on snake gall, then containing being permitted
More by the noxious material of hepatic secretion, it is also possible to there is various parasites, i.e., the snake gall modulated using white wine material is also difficult to kill and posted
It is infested.Directly with white wine rush snake gall drink absorptivity it is not high, only 5% or so.High pressure homogenizer can make the thing of suspension state
Material flows through at a high speed the cavity volume with special internal structure (high-pressure homogeneous under super-pressure (reaching as high as 60000psi) effect
Chamber), make material that a series of changes such as physics, chemistry, structural property to occur, it is finally reached the effect of homogeneous.The present invention is using high
Pressure homogenizer homogeneous snake bile, dissolution active ingredient is different only to lean on soaking in Chinese liquor, sovent diffusion from traditional snake bile, and high pressure is equal
Material can be directly crushed to cellular level by the powerful shearing force and impulsive force that matter method is produced, compared to existing technology, present invention effect
Really good, absorptivity is more than 95%.
3rd, the present invention is through development test finds repeatedly many times：Through scientific allocation in tealeaves, the eye of appropriate component is added
Mirror snake, shuilu snake gall juice, have given full play to the special efficacy of snake gall and tealeaves.The real snake gall clearing lung-heat played is promoted the production of body fluid, wind-damp dispelling, bright
Mesh benefit liver, promoting the circulation of qi resolving sputum, heat-clearing dissipates cold, cough with lung heat, resolving sputum, cough-relieving, clearing heat and detoxicating pharmacology effect.Especially to stomach not
The suitable, crowd of cough with lung heat has obvious therapeutic effect；There is the effect of resolving sputum, cough-relieving to the people of long-term smoking.
Specific embodiment
With embodiment, the invention will be further described below, but the invention is not limited in these embodiments.
Embodiment 1：
A kind of preparation method of Lingshan snake gallbladder tea, comprises the following steps：
1) snake gall source is chosen：From pollution-free foster She Chang, propagate more than 12 months time, 2 kilograms of body weight, health artificially by force
Strong high-quality, the cobra without parasite, mucosal rat snake；
2) snake gall is extracted：Snake gall is taken out after killing snake, blood stains are washed, is embathed 5 minutes with more than 60 degree of pure rice wine, put
Put in glass container is got ready, soaked 1 month with more than 60 degree of pure rice wines, it is stand-by；
3) snake gall treatment：
1. by step 2) soaked snake gall taking-up, puncture, extrude bile, filtering；
2. 1. obtained to step in bile according to 1:20 weight is well mixed than adding pure water；
3. sterilization is carried out with the sealing snake gall water that is 2. obtained to step of high-temperature steam, 160 DEG C of steam treatment 20 minutes, so
High pressure homogenizer homogeneous, 10000psi, 5min are added afterwards；
4. cobra, the mucosal rat snake snake gall water that will 3. be processed through step are according to 3:1 weight is configured to snake than well mixed
Courage mother liquor is standby；
5. the snake gall mother liquor pure water for 4. step being obtained is according to 1:20 weight is standby than dilution；
4) snake gall liquid mixes with sample tea：By step 3) the snake gall liquid that obtains, it is vaporific to be sprayed at the non-harmful finished product of high-quality
On black tea, stir and allow it to fully absorb 3 hours, bakee snake gallbladder tea.
Embodiment 2：
A kind of preparation method of Lingshan snake gallbladder tea, comprises the following steps：
1) snake gall source is chosen：From pollution-free foster She Chang, propagate more than 12 months time, 2 kilograms of body weight, health artificially by force
Strong high-quality, the cobra without parasite, mucosal rat snake；
2) snake gall is extracted：Snake gall is taken out after killing snake, blood stains are washed, is embathed 5 minutes with more than 60 degree of pure rice wine, put
Put in glass container is got ready, soaked 1 month with more than 60 degree of pure rice wines, it is stand-by；
3) snake gall treatment：
1. by step 2) soaked snake gall taking-up, puncture, extrude bile, filtering；
2. 1. obtained to step in bile according to 1:20 weight is well mixed than adding pure water；
3. sterilization is carried out with the sealing snake gall water that is 2. obtained to step of high-temperature steam, 160 DEG C of steam treatment 20 minutes, so
High pressure homogenizer homogeneous, 10000psi, 5min are added afterwards；
4. cobra, the mucosal rat snake snake gall water that will 3. be processed through step are according to 3:1 weight is configured to snake than well mixed
Courage mother liquor is standby；
5. the snake gall mother liquor pure water for 4. step being obtained is according to 1:20 weight is standby than dilution；
4) snake gall liquid mixes with sample tea：By step 3) the snake gall liquid that obtains, it is vaporific to be sprayed at the non-harmful finished product of high-quality
On green tea, stir and allow it to fully absorb 3 hours, bakee snake gallbladder tea.
Embodiment 3：
A kind of preparation method of Lingshan snake gallbladder tea, comprises the following steps：
1) snake gall source is chosen：From pollution-free foster She Chang, propagate more than 12 months time, 2 kilograms of body weight, health artificially by force
Strong high-quality, the cobra without parasite, mucosal rat snake；
2) snake gall is extracted：Snake gall is taken out after killing snake, blood stains are washed, is embathed 5 minutes with more than 60 degree of pure rice wine, put
Put in glass container is got ready, soaked 1 month with more than 60 degree of pure rice wines, it is stand-by；
3) snake gall treatment：
1. by step 2) soaked snake gall taking-up, puncture, extrude bile, filtering；
2. 1. obtained to step in bile according to 1:20 weight is well mixed than adding pure water；
3. sterilization is carried out with the sealing snake gall water that is 2. obtained to step of high-temperature steam, 160 DEG C of steam treatment 20 minutes, so
High pressure homogenizer homogeneous, 10000psi, 5min are added afterwards；
4. cobra, the mucosal rat snake snake gall water that will 3. be processed through step are according to 3:1 weight is configured to snake than well mixed
Courage mother liquor is standby；
5. the snake gall mother liquor pure water for 4. step being obtained is according to 1:20 weight is standby than dilution；
4) snake gall liquid mixes with sample tea：By step 3) the snake gall liquid that obtains, it is vaporific to be sprayed at the non-harmful finished product of high-quality
On extra-strong tea, stir and allow it to fully absorb 3 hours, bakee snake gallbladder tea.
Comparative example 1：
1) snake gall source is chosen：From pollution-free foster She Chang, propagate more than 12 months time, 2 kilograms of body weight, health artificially by force
Strong high-quality, the cobra without parasite；
2) snake gall is extracted：Snake gall is taken out after killing snake, blood stains are washed, is embathed 5 minutes with more than 60 degree of pure rice wine, put
Put in glass container is got ready, soaked 1 month with more than 60 degree of pure rice wines, it is stand-by；
3) snake gall treatment：
1. by step 2) soaked snake gall taking-up, puncture, extrude bile, filtering；
2. 1. obtained to step in bile according to 1:40 weight is well mixed than adding pure water；
4) snake gall liquid mixes with sample tea：By step 3) the snake gall liquid that obtains, it is vaporific to be sprayed at the non-harmful finished product of high-quality
On extra-strong tea, stir and allow it to fully absorb 3 hours, bakee snake gallbladder tea.
Comparative example 2：
1) snake gall source is chosen：From pollution-free foster She Chang, propagate more than 12 months time, 2 kilograms of body weight, health artificially by force
Strong high-quality, the cobra without parasite, mucosal rat snake；
2) snake gall is extracted：Snake gall is taken out after killing snake, blood stains are washed, is embathed 5 minutes with more than 60 degree of pure rice wine, put
Put in glass container is got ready, soaked 1 month with more than 60 degree of pure rice wines, it is stand-by；
3) snake gall treatment：
1. by step 2) soaked snake gall taking-up, puncture, extrude bile, filtering；
2. 1. obtained to step in bile according to 1:20 weight is well mixed than adding pure water；
3. the snake gall water for 2. being obtained to step with sealing high-temperature steam carries out sterilization, 160 DEG C of steam treatment 20 minutes；
4. cobra, the mucosal rat snake snake gall water that will 3. be processed through step are according to 3:1 weight is configured to snake than well mixed
Courage mother liquor is standby；
5. the snake gall mother liquor pure water for 4. step being obtained is according to 1:20 weight is standby than dilution；
4) snake gall liquid mixes with sample tea：By step 3) the snake gall liquid that obtains, it is vaporific to be sprayed at the non-harmful finished product of high-quality
On extra-strong tea, stir and allow it to fully absorb 3 hours, bakee snake gallbladder tea.
Comparative example 3：
1) snake gall source is chosen：From pollution-free foster She Chang, propagate more than 12 months time, 2 kilograms of body weight, health artificially by force
Strong high-quality, the cobra without parasite；
2) snake gall is extracted：Snake gall is taken out after killing snake, blood stains are washed, is embathed 5 minutes with more than 60 degree of pure rice wine, put
Put in glass container is got ready, soaked 1 month with more than 60 degree of pure rice wines, it is stand-by；
3) snake gall treatment：
1. by step 2) soaked snake gall taking-up, puncture, extrude bile, filtering；
2. 1. obtained to step in bile according to 1:20 weight is well mixed than adding pure water；
3. sterilization is carried out with the sealing snake gall water that is 2. obtained to step of high-temperature steam, 160 DEG C of steam treatment 20 minutes, so
High pressure homogenizer homogeneous, 10000psi, 5min are added afterwards；
4. the snake gall mother liquor pure water for 4. step being obtained is according to 1:20 weight is standby than dilution；
4) snake gall liquid mixes with sample tea：By step 3) the snake gall liquid that obtains, it is vaporific to be sprayed at the non-harmful finished product of high-quality
On extra-strong tea, stir and allow it to fully absorb 3 hours, bakee snake gallbladder tea.
Disease Control and Prevention Center of present invention applicant's commission autonomous region has carried out following effect experiment：
Effect experiment 1：Intervention effect of the snake gallbladder tea to lung fibrosis in rats
1st, test specimen
The snake gallbladder tea that embodiment 3 is made
2nd, experimental animal
SD rats, male, SPF grades, body weight:220-250g
3rd, test procedure
SD rats 60 are only randomly divided into 5 groups (every group 12), respectively：Normal group, model group, prednisone acetate sun
Property control group, medicine high dose group, medicine low dose group.In addition to normal group, remaining chloraldurate of each group rats by intraperitoneal injection 4%
0.01ml/g is anaesthetized, aseptically row neck median incision, blunt separation, exposes tracheae, rich next mould to tracheal strips injection
Plain 4.5mg/kg prepares pulmonary fibrosis model, and second day gavage gives relative medicine after modeling, is administered once daily.Normal combination
Model group gives isometric physiological saline.The observation behavior of rat outward appearance and changes of weight, change are put to death after 28 days, are completely isolated
Double lungs, weigh lung weight in wet base, calculate paragonimus cyst, wherein paragonimus cyst computing formula：
4th, experimental result
1) rat outward appearance Behavioral change
Flexibly, the state of mind is good for normal rats activity, and feed water inlet is normal, and fur is glossy, increased weight.Model group is big
Mouse is short of breath, and feed water inlet is reduced, slow in reacting, aggressive low.Positive controls and medicine high and low dose group rat outward appearance
Situation is significantly better than model group.
2) snake gallbladder tea is to rat body weight, the influence of lung weight and paragonimus cyst
Experimental data represents that data statistics is carried out with SPSS11.0 softwares with (X ± s), is compareed using one way between group
Anova is carried out.
Influence of the snake gallbladder tea of table 1 to lung fibrosis in rats
Note：The P of ▲ ▲ compared with normal group<0.01, * * P compared with model group<0.01, * compared with model group P<0.05
5th, experimental analysis and conclusion
As shown in Table 1 after modeling 28 days, compared with normal group, model group rats body weight is decreased obviously (P<0.01), lung system
Digital display writes and raises (P<0.01) modeling success, is illustrated, morphological observation shows that each dosage group state of appearance of snake gallbladder tea is substantially better than
Model group, and medicine high and low dose group and prednisone acetate positive controls can in various degree increase the body of lung fibrosis in rats
Weight (P<0.05, P<0.01) paragonimus cyst (P, is significantly reduced<0.01), thus illustrate the snake gallbladder tea being made of the invention to pulmonary fibrosis
There is certain therapeutic action.
Effect experiment 2：The research that snake gallbladder tea is intervened COPD rat
1st, test specimen
The snake gallbladder tea that embodiment is made
2nd, experimental animal
Wistar rats, male, SPF grades, body weight 240-260g
3rd, experimental procedure
30 Wistar rats are randomly divided into 3 groups (every group 10), respectively：Normal group, model group, medicine group.Its
Middle model group and medicine group prepare COPD rat models using " sootiness joint air flue instills lipopolysaccharides ".Medicine group gavage gives this
The normal group snake gallbladder tea that invention is made, normal group and model group give isometric physiological saline.Three groups of rat metamorphosis are observed,
Rat is put to death in gavage intervention after 30 days, lung tissue row H-E dyeing, observation lung morphology changes, between calculating the average liner of lung
Every number (MLI), average alveolar number (MAN), and estimate the degree of each group rat pulmonary emphysema.
4th, experimental result
Snake gallbladder tea such as can improve the COPD rat models state of mind and cough, expectoration, pant at the symptom.
Three groups of lung tissue of rats Pathologic changes：Normal rats pathologic shows：Without obvious inflammatory cell infiltration,
Without thickening, without coming off, without thinning, dangerous pulmonary belb is formed alveolar wall bronchiolar epithelium cilium marshalling tunica mucosa bronchiorum；Mould
The visible inflammatory cell infiltration of type group lung tissue of rats, it is seen that bronchial wall is thickened, and cilium comes off, visible mucous plug is formed in air flue
And chronic inflammatory cell lung infiltration.Medicine group also has model group sample to change, but compared with model group inflammatory cell infiltration mild degree, pulmonary emphysema
Leaflet destruction is few compared with model group.
Three groups of average liners interval (MLI) of induced lung are compared：The average liner space-number of normal rats lung：(8.75±
0.62)×10-5M, model group (14.15 ± 0.71) × 10-5M, medicine group (11.12 ± 0.78) × 10-5m.Model group with it is normal
Group compares P<0.01, there is statistically-significant difference, illustrate modeling success, medicine group P compared with model group<0.05, there is statistics
Difference, shows that snake gallbladder tea can obviously improve chronic obstructive pneumonia.
Three groups of rat average alveolars number (MAN) are compared：Normal group average alveolar number (12.11 ± 2.76) × 107/m2, model
Group：(5.18±0.99)×107/m2, medicine group：(7.33±1.56)×107/m2.Wherein model group P compared with normal group<
0.01, have statistically-significant difference, illustrate model group rats alveolar density compared with normal group reduction, modeling success, and medicine group with
Model group compares P<0.05, there is significant difference, alveolar density is high compared with model group after illustrating to intervene through snake gallbladder tea, there is certain
Improve symptom effect.
5th, experimental analysis and conclusion
From above experimental result, the snake gallbladder tea that the present invention is made can improve COPD rat model clinical symptoms, can
Slow down the development process of pulmonary emphysema to suppress COPD rat model lung tissue inflammatory reactions, point out snake gallbladder tea to improve chronic
The clinical symptoms of obstructive pneumonia, PFT mitigates carbon dioxide retention, improves the quality of life of sub-health population.
Contrast experiment：
In experiment, the contrast experiment of snake gallbladder tea that comparative example obtains to the intervention effect of lung fibrosis in rats has also been.
Efficacy experiments method and step is with the test of pesticide effectiveness 1.
Influence of the snake gallbladder tea of table 2 to lung fibrosis in rats
Note：The P of ▲ ▲ compared with normal group<0.01, * * P compared with model group<0.01, * compared with model group P<0.05
Contrast experiment's conclusion
The experimental result of embodiment 3 is substantially better than the result of contrast experiment.
Claims (6)
1. a kind of preparation method of Lingshan snake gallbladder tea, it is characterised in that comprise the following steps：
1) snake gall source is chosen：From pollution-free foster She Chang, propagate the snake of time, healthy strong high-quality artificially；
2) snake gall is extracted：Snake gall is taken out after killing snake, blood stains are washed, embathed in wine, be placed in getting container ready, use steeping in wine
Bubble, it is stand-by；
3) snake gall treatment：
1. by step 2) soaked snake gall taking-up, puncture, extrude bile, filtering；
2. 1. obtain adding pure water in bile to step, be well mixed；
3. the snake gall water for 2. being obtained to step with sealing high-temperature steam carries out sterilization, is subsequently adding high pressure homogenizer homogeneous；
4. the cobra 3. processed through step, mucosal rat snake snake gall water are well mixed, are configured to snake gall mother liquor standby；
5. the snake gall mother liquor pure water dilution for 4. step being obtained, it is standby；
4) snake gall liquid mixes with sample tea：By step 3) the snake gall liquid that obtains, it is vaporific to be sprayed in sample tea, stir and make its abundant
Absorb, bakee snake gallbladder tea.
2. the preparation method of a kind of Lingshan snake gallbladder tea according to claim 1, it is characterised in that：Step 1) described in snake,
It is artificial culturing time more than 12 months, 2 kilograms of body weight, healthy strong high-quality, the snake without parasite, cultivar origin is in glasses
Snake, mucosal rat snake.
3. the preparation method of a kind of Lingshan snake gallbladder tea according to claim 1, it is characterised in that：Step 2) described in extraction
Snake gall, refers to take out snake gall after killing snake, washes blood stains, is embathed 5 minutes with more than 60 degree of pure rice wine, is placed on and gets glass ready
In glass container, soaked 1 month with more than 60 degree of pure rice wines, it is stand-by.
4. the preparation method of a kind of Lingshan snake gallbladder tea according to claim 1, it is characterised in that：Step 3) 2. in, institute
1. being obtained to step of stating adds pure water in bile, according to 1:20 weight ratio；The 3. described sterilization of step, is 160 DEG C
Steam treatment 20 minutes, described high pressure homogenizer homogeneous, is 10000psi, 5min；Step 4. described in cobra, slide-mouse
Calmy courage water is well mixed, according to 3:1 weight ratio；The 5. described snake gall mother liquor pure water for 4. obtaining step of step is dilute
Release, according to 1:20 weight ratio.
5. the preparation method of a kind of Lingshan snake gallbladder tea according to claim 1, it is characterised in that：Step 4) described in it is abundant
Absorb, be 3-4 hours；Described step 3) the snake gall liquid that obtains and sample tea, according to 1:100 weight ratio；Described finished product
Tea, is the one kind in the non-harmful produced black tea of high-quality, green tea or extra-strong tea.
6. the snake gallbladder tea for being obtained according to any described preparation methods of claim 1-5.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110050860A (en) * | 2019-05-16 | 2019-07-26 | 黄文枝 | A kind of snake gallbladder tea and preparation method thereof |
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|---|---|---|---|---|
| CN1063203A (en) * | 1991-01-19 | 1992-08-05 | 索连江 | Processing method of gentian tea |
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2017
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1063203A (en) * | 1991-01-19 | 1992-08-05 | 索连江 | Processing method of gentian tea |
Non-Patent Citations (2)
| Title |
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| 中国轻工总会编: "《轻工业技术装备手册 第3卷》", 31 August 1997, 机械工业出版社 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110050860A (en) * | 2019-05-16 | 2019-07-26 | 黄文枝 | A kind of snake gallbladder tea and preparation method thereof |
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Inventor after: Feng Dejin Inventor after: Wang Jie Inventor after: Yang Guangxing Inventor after: Teng Jiashu Inventor before: Feng Dejin Inventor before: Yang Guangxing Inventor before: Teng Jiashu |
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Application publication date: 20170531 |