CN106720245A - A kind of rascal mango preservative and preservation method - Google Patents
A kind of rascal mango preservative and preservation method Download PDFInfo
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- CN106720245A CN106720245A CN201610383326.6A CN201610383326A CN106720245A CN 106720245 A CN106720245 A CN 106720245A CN 201610383326 A CN201610383326 A CN 201610383326A CN 106720245 A CN106720245 A CN 106720245A
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- extract
- ethanol
- rascal
- lysozyme
- butyl alcohol
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- 235000004936 Bromus mango Nutrition 0.000 title claims abstract description 30
- 235000014826 Mangifera indica Nutrition 0.000 title claims abstract description 30
- 235000009184 Spondias indica Nutrition 0.000 title claims abstract description 30
- 239000003755 preservative agent Substances 0.000 title claims abstract description 18
- 230000002335 preservative effect Effects 0.000 title claims abstract description 18
- 238000004321 preservation Methods 0.000 title claims abstract description 8
- 238000000034 method Methods 0.000 title claims abstract description 7
- 240000007228 Mangifera indica Species 0.000 title 1
- 239000000284 extract Substances 0.000 claims abstract description 117
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 64
- 241000830535 Ligustrum lucidum Species 0.000 claims abstract description 41
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 41
- 102000016943 Muramidase Human genes 0.000 claims abstract description 38
- 108010014251 Muramidase Proteins 0.000 claims abstract description 38
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims abstract description 38
- 239000004325 lysozyme Substances 0.000 claims abstract description 38
- 235000010335 lysozyme Nutrition 0.000 claims abstract description 38
- 229960000274 lysozyme Drugs 0.000 claims abstract description 38
- 241000100289 Lonicera confusa Species 0.000 claims abstract description 30
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 29
- 241001093152 Mangifera Species 0.000 claims abstract description 29
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 29
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 29
- 239000000661 sodium alginate Substances 0.000 claims abstract description 29
- 241000206575 Chondrus crispus Species 0.000 claims abstract description 19
- 108010010803 Gelatin Proteins 0.000 claims abstract description 19
- 229920000159 gelatin Polymers 0.000 claims abstract description 19
- 239000008273 gelatin Substances 0.000 claims abstract description 19
- 235000019322 gelatine Nutrition 0.000 claims abstract description 19
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 19
- 229920001285 xanthan gum Polymers 0.000 claims abstract description 19
- 230000000694 effects Effects 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 168
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 162
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 90
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 36
- 239000012141 concentrate Substances 0.000 claims description 36
- 239000003208 petroleum Substances 0.000 claims description 36
- 238000002360 preparation method Methods 0.000 claims description 34
- 239000000243 solution Substances 0.000 claims description 31
- 239000000706 filtrate Substances 0.000 claims description 27
- 239000011259 mixed solution Substances 0.000 claims description 19
- 238000010828 elution Methods 0.000 claims description 18
- 238000002481 ethanol extraction Methods 0.000 claims description 18
- 239000012259 ether extract Substances 0.000 claims description 18
- 238000001914 filtration Methods 0.000 claims description 18
- 239000012467 final product Substances 0.000 claims description 18
- 238000011010 flushing procedure Methods 0.000 claims description 18
- 239000011347 resin Substances 0.000 claims description 18
- 229920005989 resin Polymers 0.000 claims description 18
- 229920006395 saturated elastomer Polymers 0.000 claims description 18
- 238000001694 spray drying Methods 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 12
- 241001570521 Lonicera periclymenum Species 0.000 claims description 10
- 239000003480 eluent Substances 0.000 claims description 9
- 239000000725 suspension Substances 0.000 claims description 9
- 241000196324 Embryophyta Species 0.000 claims description 7
- 240000005979 Hordeum vulgare Species 0.000 claims description 4
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 4
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical group CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims description 3
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010000912 Egg Proteins Proteins 0.000 claims description 3
- 244000088415 Raphanus sativus Species 0.000 claims description 3
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 claims description 3
- 235000014103 egg white Nutrition 0.000 claims description 3
- 210000000969 egg white Anatomy 0.000 claims description 3
- 238000004821 distillation Methods 0.000 claims 2
- 238000002156 mixing Methods 0.000 claims 2
- 239000003795 chemical substances by application Substances 0.000 abstract description 20
- 238000010790 dilution Methods 0.000 description 16
- 239000012895 dilution Substances 0.000 description 16
- 239000000047 product Substances 0.000 description 9
- 230000008021 deposition Effects 0.000 description 8
- 239000003292 glue Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- GJCOSYZMQJWQCA-UHFFFAOYSA-N 9H-xanthene Chemical compound C1=CC=C2CC3=CC=CC=C3OC2=C1 GJCOSYZMQJWQCA-UHFFFAOYSA-N 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000189799 Asimina triloba Species 0.000 description 1
- 235000006264 Asimina triloba Nutrition 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 235000000540 Brassica rapa subsp rapa Nutrition 0.000 description 1
- 235000009467 Carica papaya Nutrition 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 206010023126 Jaundice Diseases 0.000 description 1
- 230000003260 anti-sepsis Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000019249 food preservative Nutrition 0.000 description 1
- 239000005452 food preservative Substances 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
- A23B7/155—Microorganisms; Enzymes; Antibiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a kind of rascal mango preservative and preservation method, by weight, the water per 1L includes 1.5~2.5g of sodium alginate, 5.5~7.5g of gelatin, 2~4g of carragheen, 2~4g of xanthans, 0.5~1.5g of lysozyme to antistaling agent；Glossy privet fruit extract and Lonicera confusa extract totally 30~50g, the two weight ratio are 2~4:1.When using, rascal mango impregnated in described antistaling agent and dried after 5~10min.The antistaling agent that the present invention is provided can effectively delay rascal mango to turn yellow, and improve fresh-keeping effect, and this may be relevant with the weight ratio of glossy privet fruit extract in raw material and Lonicera confusa extract.
Description
Technical field
The invention belongs to field of food, and in particular to a kind of rascal mango preservative and preservation method.
Background technology
Food preservative is segmented into two major classes, i.e. chemical synthesis class and natural class antistaling agent.For a long time, people are main
Using chemical synthesis class antistaling agent to store preserving fruit and vegetable utilizing.Although the preservation and antisepsis effect of chemical synthesis class antistaling agent is preferably,
But many synthetics confrontation healths have certain toxic and side effect.Therefore, people start notice to turn to natural class
Antistaling agent, natural plant extracts turn into the emphasis of people's research concern.
The content of the invention
It is an object of the invention to provide a kind of rascal mango preservative and preservation method.
Above-mentioned purpose is achieved by the following technical solution：
A kind of rascal mango preservative, comprising following raw material：By weight, per 1L water comprising sodium alginate 1.5~
2.5g, 5.5~7.5g of gelatin, 2~4g of carragheen, 2~4g of xanthans, 0.5~1.5g of lysozyme；Glossy privet fruit extract and Shan Yin
Flower extract totally 30~50g, the two weight ratio is 2~4:1；
The preparation method of the glossy privet fruit extract is：A () crushes the fruit of glossy privet, carried with the heat backflow of 75% ethanol solution
Take, merging filtrate, be concentrated into and obtain ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate water by ()
Dilution, successively with petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, be concentrated under reduced pressure, respectively obtain petroleum ether extract,
Acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolves, filtering, filtrate uses D101 macroreticular resins
Deposition activity composition, first removes big polar components with 10% alcohol flushing, 8 column volumes, then with 70% ethanol elution, 10 cylinders
Product, collects 70% ethanol eluate, is concentrated under reduced pressure, and spray drying is obtained final product；
The preparation method of the Lonicera confusa extract is：A () crushes Honeysuckle flower, carried with the heat backflow of 60% ethanol solution
Take, merging filtrate, be concentrated into and obtain ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate water by ()
Dilution, successively with petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, be concentrated under reduced pressure, respectively obtain petroleum ether extract,
Acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolves, filtering, use AB-8 macroporous resin enrichments
Active component, first removes big polar components with 10% alcohol flushing, 6 column volumes, then with 55% ethanol elution, 10 column volumes,
55% eluent is collected, is concentrated under reduced pressure, spray drying is obtained final product.
Further, the lysozyme is the mixture of one or more in egg white lysozyme and plant lysozyme.
Further, the plant lysozyme is radish lysozyme or barley lysozyme.
The preparation method of above-mentioned rascal mango preservative, comprises the following steps：(1) sodium alginate soln is prepared, to described
Gelatin, carragheen and xanthans is added to be mixed to form colloidal sol in sodium alginate soln；(2) glossy privet fruit extract and Honeysuckle flower are carried
Take thing and be configured to extract solution, add lysozyme to form mixed solution after being well mixed；(3) mixed solution is slowly added
Enter and be mixed to form suspension in the colloidal sol.
Further, in step (3), the speed that the mixed solution is added in the colloidal sol is 0.5~1mL/min.
A kind of rascal preservation method for mangoes：Rascal mango impregnated in above-mentioned antistaling agent and dried after 5~10min.
Beneficial effects of the present invention：
The antistaling agent that the present invention is provided can improve the fresh-keeping effect of rascal mango, effectively delay that rascal mango turns yellow when
Between.
Specific embodiment
Lysozyme in the raw material of rascal mango preservative of the present invention is in egg white lysozyme or plant lysozyme
One or more of mixture；Wherein plant lysozyme can be from plants such as pawpaw, fig, turnip, barley, radish, cucumber
In the lysozyme isolated, following embodiments use barley lysozyme.Agents useful for same of the present invention, material are without specified otherwise
Outward, conventional reagent, the material that can be directly bought from market are.Ethanol is food-grade ethanol.It is the sodium alginate that uses, various
Glue class is food grade raw material, meets national standard.The lysozyme for using is analysis net product.
The ethanol solution of various concentrations is concentration expressed in percentage by volume.
Embodiment 1：The preparation of rascal mango preservative and use
Comprising following raw material：By weight, the water per 1L includes sodium alginate 2g, gelatin 6.5g, carragheen 3g, xanthans
3g, lysozyme 1g；Glossy privet fruit extract and the common 40g of Lonicera confusa extract, the two weight ratio are 3:1；
The preparation method of the glossy privet fruit extract is：A () crushes the fruit of glossy privet, carried with the heat backflow of 75% ethanol solution
Take, merging filtrate, be concentrated into and obtain ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate water by ()
Dilution, successively with petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, be concentrated under reduced pressure, respectively obtain petroleum ether extract,
Acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolves, filtering, filtrate uses D101 macroreticular resins
Deposition activity composition, first removes big polar components with 10% alcohol flushing, 8 column volumes, then with 70% ethanol elution, 10 cylinders
Product, collects 70% ethanol eluate, is concentrated under reduced pressure, and spray drying is obtained final product；
The preparation method of the Lonicera confusa extract is：A () crushes Honeysuckle flower, carried with the heat backflow of 60% ethanol solution
Take, merging filtrate, be concentrated into and obtain ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate water by ()
Dilution, successively with petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, be concentrated under reduced pressure, respectively obtain petroleum ether extract,
Acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolves, filtering, use AB-8 macroporous resin enrichments
Active component, first removes big polar components with 10% alcohol flushing, 6 column volumes, then with 55% ethanol elution, 10 column volumes,
55% eluent is collected, is concentrated under reduced pressure, spray drying is obtained final product.
The preparation method of antistaling agent：
Comprise the following steps：(1) sodium alginate soln is prepared, to addition gelatin, carragheen in the sodium alginate soln
Colloidal sol is mixed to form with xanthans；(2) glossy privet fruit extract and Lonicera confusa extract are configured to extract solution, are well mixed
Lysozyme is added to form mixed solution afterwards；(3) mixed solution is slowly added to be mixed to form suspension i.e. in the colloidal sol
Can.
Embodiment 2：The preparation of rascal mango preservative and use
Comprising following raw material：By weight, the water per 1L includes sodium alginate 1.5g, gelatin 5.5g, carragheen 2g, xanthan
Glue 2g, lysozyme 0.5g；Glossy privet fruit extract and the common 30g of Lonicera confusa extract, the two weight ratio are 2:1；
The preparation method of the glossy privet fruit extract is：A () crushes the fruit of glossy privet, carried with the heat backflow of 75% ethanol solution
Take, merging filtrate, be concentrated into and obtain ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate water by ()
Dilution, successively with petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, be concentrated under reduced pressure, respectively obtain petroleum ether extract,
Acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolves, filtering, filtrate uses D101 macroreticular resins
Deposition activity composition, first removes big polar components with 10% alcohol flushing, 8 column volumes, then with 70% ethanol elution, 10 cylinders
Product, collects 70% ethanol eluate, is concentrated under reduced pressure, and spray drying is obtained final product；
The preparation method of the Lonicera confusa extract is：A () crushes Honeysuckle flower, carried with the heat backflow of 60% ethanol solution
Take, merging filtrate, be concentrated into and obtain ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate water by ()
Dilution, successively with petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, be concentrated under reduced pressure, respectively obtain petroleum ether extract,
Acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolves, filtering, use AB-8 macroporous resin enrichments
Active component, first removes big polar components with 10% alcohol flushing, 6 column volumes, then with 55% ethanol elution, 10 column volumes,
55% eluent is collected, is concentrated under reduced pressure, spray drying is obtained final product.
The preparation method of antistaling agent：
Comprise the following steps：(1) sodium alginate soln is prepared, to addition gelatin, carragheen in the sodium alginate soln
Colloidal sol is mixed to form with xanthans；(2) glossy privet fruit extract and Lonicera confusa extract are configured to extract solution, are well mixed
Lysozyme is added to form mixed solution afterwards；(3) mixed solution is slowly added to be mixed to form suspension i.e. in the colloidal sol
Can.
Embodiment 3：The preparation of rascal mango preservative and use
Comprising following raw material：By weight, the water per 1L includes sodium alginate 2.5g, gelatin 7.5g, carragheen 4g, xanthan
Glue 4g, lysozyme 1.5g；Glossy privet fruit extract and the common 50g of Lonicera confusa extract, the two weight ratio are 4:1；
The preparation method of the glossy privet fruit extract is：A () crushes the fruit of glossy privet, carried with the heat backflow of 75% ethanol solution
Take, merging filtrate, be concentrated into and obtain ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate water by ()
Dilution, successively with petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, be concentrated under reduced pressure, respectively obtain petroleum ether extract,
Acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolves, filtering, filtrate uses D101 macroreticular resins
Deposition activity composition, first removes big polar components with 10% alcohol flushing, 8 column volumes, then with 70% ethanol elution, 10 cylinders
Product, collects 70% ethanol eluate, is concentrated under reduced pressure, and spray drying is obtained final product；
The preparation method of the Lonicera confusa extract is：A () crushes Honeysuckle flower, carried with the heat backflow of 60% ethanol solution
Take, merging filtrate, be concentrated into and obtain ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate water by ()
Dilution, successively with petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, be concentrated under reduced pressure, respectively obtain petroleum ether extract,
Acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolves, filtering, use AB-8 macroporous resin enrichments
Active component, first removes big polar components with 10% alcohol flushing, 6 column volumes, then with 55% ethanol elution, 10 column volumes,
55% eluent is collected, is concentrated under reduced pressure, spray drying is obtained final product.
The preparation method of antistaling agent：
Comprise the following steps：(1) sodium alginate soln is prepared, to addition gelatin, carragheen in the sodium alginate soln
Colloidal sol is mixed to form with xanthans；(2) glossy privet fruit extract and Lonicera confusa extract are configured to extract solution, are well mixed
Lysozyme is added to form mixed solution afterwards；(3) mixed solution is slowly added to be mixed to form suspension i.e. in the colloidal sol
Can.
Embodiment 4：The preparation of rascal mango preservative and use
Comprising following raw material：By weight, the water per 1L includes sodium alginate 2g, gelatin 6.5g, carragheen 3g, xanthans
3g, lysozyme 1g；Glossy privet fruit extract and the common 40g of Lonicera confusa extract, the two weight ratio are 2:1；
The preparation method of the glossy privet fruit extract is：A () crushes the fruit of glossy privet, carried with the heat backflow of 75% ethanol solution
Take, merging filtrate, be concentrated into and obtain ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate water by ()
Dilution, successively with petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, be concentrated under reduced pressure, respectively obtain petroleum ether extract,
Acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolves, filtering, filtrate uses D101 macroreticular resins
Deposition activity composition, first removes big polar components with 10% alcohol flushing, 8 column volumes, then with 70% ethanol elution, 10 cylinders
Product, collects 70% ethanol eluate, is concentrated under reduced pressure, and spray drying is obtained final product；
The preparation method of the Lonicera confusa extract is：A () crushes Honeysuckle flower, carried with the heat backflow of 60% ethanol solution
Take, merging filtrate, be concentrated into and obtain ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate water by ()
Dilution, successively with petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, be concentrated under reduced pressure, respectively obtain petroleum ether extract,
Acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolves, filtering, use AB-8 macroporous resin enrichments
Active component, first removes big polar components with 10% alcohol flushing, 6 column volumes, then with 55% ethanol elution, 10 column volumes,
55% eluent is collected, is concentrated under reduced pressure, spray drying is obtained final product.
The preparation method of antistaling agent：
Comprise the following steps：(1) sodium alginate soln is prepared, to addition gelatin, carragheen in the sodium alginate soln
Colloidal sol is mixed to form with xanthans；(2) glossy privet fruit extract and Lonicera confusa extract are configured to extract solution, are well mixed
Lysozyme is added to form mixed solution afterwards；(3) mixed solution is slowly added to be mixed to form suspension i.e. in the colloidal sol
Can.
Embodiment 5：The preparation of rascal mango preservative and use
Comprising following raw material：By weight, the water per 1L includes sodium alginate 2g, gelatin 6.5g, carragheen 3g, xanthans
3g, lysozyme 1g；Glossy privet fruit extract and the common 40g of Lonicera confusa extract, the two weight ratio are 4:1；
The preparation method of the glossy privet fruit extract is：A () crushes the fruit of glossy privet, carried with the heat backflow of 75% ethanol solution
Take, merging filtrate, be concentrated into and obtain ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate water by ()
Dilution, successively with petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, be concentrated under reduced pressure, respectively obtain petroleum ether extract,
Acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolves, filtering, filtrate uses D101 macroreticular resins
Deposition activity composition, first removes big polar components with 10% alcohol flushing, 8 column volumes, then with 70% ethanol elution, 10 cylinders
Product, collects 70% ethanol eluate, is concentrated under reduced pressure, and spray drying is obtained final product；
The preparation method of the Lonicera confusa extract is：A () crushes Honeysuckle flower, carried with the heat backflow of 60% ethanol solution
Take, merging filtrate, be concentrated into and obtain ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate water by ()
Dilution, successively with petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, be concentrated under reduced pressure, respectively obtain petroleum ether extract,
Acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolves, filtering, use AB-8 macroporous resin enrichments
Active component, first removes big polar components with 10% alcohol flushing, 6 column volumes, then with 55% ethanol elution, 10 column volumes,
55% eluent is collected, is concentrated under reduced pressure, spray drying is obtained final product.
The preparation method of antistaling agent：
Comprise the following steps：(1) sodium alginate soln is prepared, to addition gelatin, carragheen in the sodium alginate soln
Colloidal sol is mixed to form with xanthans；(2) glossy privet fruit extract and Lonicera confusa extract are configured to extract solution, are well mixed
Lysozyme is added to form mixed solution afterwards；(3) mixed solution is slowly added to be mixed to form suspension i.e. in the colloidal sol
Can.
Embodiment 6：Comparative example, glossy privet fruit extract and Lonicera confusa extract weight ratio are 1:1
Comprising following raw material：By weight, the water per 1L includes sodium alginate 2g, gelatin 6.5g, carragheen 3g, xanthans
3g, lysozyme 1g；Glossy privet fruit extract and the common 40g of Lonicera confusa extract, the two weight ratio are 1:1；
The preparation method of the glossy privet fruit extract is：A () crushes the fruit of glossy privet, carried with the heat backflow of 75% ethanol solution
Take, merging filtrate, be concentrated into and obtain ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate water by ()
Dilution, successively with petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, be concentrated under reduced pressure, respectively obtain petroleum ether extract,
Acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolves, filtering, filtrate uses D101 macroreticular resins
Deposition activity composition, first removes big polar components with 10% alcohol flushing, 8 column volumes, then with 70% ethanol elution, 10 cylinders
Product, collects 70% ethanol eluate, is concentrated under reduced pressure, and spray drying is obtained final product；
The preparation method of the Lonicera confusa extract is：A () crushes Honeysuckle flower, carried with the heat backflow of 60% ethanol solution
Take, merging filtrate, be concentrated into and obtain ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate water by ()
Dilution, successively with petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, be concentrated under reduced pressure, respectively obtain petroleum ether extract,
Acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolves, filtering, use AB-8 macroporous resin enrichments
Active component, first removes big polar components with 10% alcohol flushing, 6 column volumes, then with 55% ethanol elution, 10 column volumes,
55% eluent is collected, is concentrated under reduced pressure, spray drying is obtained final product.
The preparation method of antistaling agent：
Comprise the following steps：(1) sodium alginate soln is prepared, to addition gelatin, carragheen in the sodium alginate soln
Colloidal sol is mixed to form with xanthans；(2) glossy privet fruit extract and Lonicera confusa extract are configured to extract solution, are well mixed
Lysozyme is added to form mixed solution afterwards；(3) mixed solution is slowly added to be mixed to form suspension i.e. in the colloidal sol
Can.
Embodiment 7：Comparative example, glossy privet fruit extract and Lonicera confusa extract weight ratio are 5:1
Comprising following raw material：By weight, the water per 1L includes sodium alginate 2g, gelatin 6.5g, carragheen 3g, xanthans
3g, lysozyme 1g；Glossy privet fruit extract and the common 40g of Lonicera confusa extract, the two weight ratio are 5:1；
The preparation method of the glossy privet fruit extract is：A () crushes the fruit of glossy privet, carried with the heat backflow of 75% ethanol solution
Take, merging filtrate, be concentrated into and obtain ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate water by ()
Dilution, successively with petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, be concentrated under reduced pressure, respectively obtain petroleum ether extract,
Acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolves, filtering, filtrate uses D101 macroreticular resins
Deposition activity composition, first removes big polar components with 10% alcohol flushing, 8 column volumes, then with 70% ethanol elution, 10 cylinders
Product, collects 70% ethanol eluate, is concentrated under reduced pressure, and spray drying is obtained final product；
The preparation method of the Lonicera confusa extract is：A () crushes Honeysuckle flower, carried with the heat backflow of 60% ethanol solution
Take, merging filtrate, be concentrated into and obtain ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate water by ()
Dilution, successively with petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, be concentrated under reduced pressure, respectively obtain petroleum ether extract,
Acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolves, filtering, use AB-8 macroporous resin enrichments
Active component, first removes big polar components with 10% alcohol flushing, 6 column volumes, then with 55% ethanol elution, 10 column volumes,
55% eluent is collected, is concentrated under reduced pressure, spray drying is obtained final product.
The preparation method of antistaling agent：
Comprise the following steps：(1) sodium alginate soln is prepared, to addition gelatin, carragheen in the sodium alginate soln
Colloidal sol is mixed to form with xanthans；(2) glossy privet fruit extract and Lonicera confusa extract are configured to extract solution, are well mixed
Lysozyme is added to form mixed solution afterwards；(3) mixed solution is slowly added to be mixed to form suspension i.e. in the colloidal sol
Can.
Embodiment 8：Effect example
Rascal mango will influence sale once turning to be yellow.To test the fresh-keeping effect of antistaling agent of the present invention, respectively by rascal
Mango is dried after 5~10min in impregnated in the antistaling agent of the preparation of embodiment 1~7, and room temperature storage, observation mango starts jaundice
Time.
Result such as following table.
The above results show that the antistaling agent that the present invention is provided can effectively delay rascal mango to turn yellow, and improve fresh-keeping effect,
This may be relevant with the weight ratio of glossy privet fruit extract in raw material and Lonicera confusa extract.
The effect of above-described embodiment indicates that essentiality content of the invention, but with this does not limit protection of the invention
Scope.It will be understood by those within the art that, technical scheme can be modified or equivalent,
Without deviating from the essence and protection domain of technical solution of the present invention.
Claims (6)
1. a kind of rascal mango preservative, it is characterised in that comprising following raw material：By weight, the water per 1L includes sodium alginate
1.5~2.5g, 5.5~7.5g of gelatin, 2~4g of carragheen, 2~4g of xanthans, 0.5~1.5g of lysozyme；Glossy privet fruit extract
With Lonicera confusa extract totally 30~50g, the two weight ratio is 2~4:1；
The preparation method of the glossy privet fruit extract is：A () crushes the fruit of glossy privet, with 75% ethanol solution circumfluence distillation, close
And filtrate, it is concentrated into and obtains ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate dilute with water by (),
Petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol are used successively, is concentrated under reduced pressure, respectively obtain petroleum ether extract, acetic acid
Ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolves, filtering, filtrate uses D101 macroporous resin enrichments
Active component, first removes big polar components with 10% alcohol flushing, 8 column volumes, then with 70% ethanol elution, 10 column volumes,
70% ethanol eluate is collected, is concentrated under reduced pressure, spray drying is obtained final product；
The preparation method of the Lonicera confusa extract is：A () crushes Honeysuckle flower, with 60% ethanol solution circumfluence distillation, close
And filtrate, it is concentrated into and obtains ethanol extraction concentrate without alcohol taste；B step (a) gained ethanol is extracted concentrate dilute with water by (),
Petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol are used successively, is concentrated under reduced pressure, respectively obtain petroleum ether extract, acetic acid
Ethyl ester extract and n-butyl alcohol extract；(c) n-butyl alcohol extract water dissolves, filtering, with AB-8 macroporous resin enrichments activity
Composition, first removes big polar component, then with 55% ethanol elution, 10 column volumes, collect with 10% alcohol flushing, 6 column volumes
55% eluent, is concentrated under reduced pressure, and spray drying is obtained final product.
2. rascal mango preservative according to claim 1, it is characterised in that：The lysozyme is egg white lysozyme and plant
One or more in thing lysozyme of mixture.
3. rascal mango preservative according to claim 2, it is characterised in that：The plant lysozyme is radish lysozyme
Or barley lysozyme.
4. the preparation method of any rascal mango preservative of claims 1 to 3, it is characterised in that comprise the following steps：
(1) sodium alginate soln is prepared, colloidal sol is mixed to form to gelatin, carragheen and xanthans is added in the sodium alginate soln；
(2) glossy privet fruit extract and Lonicera confusa extract are configured to extract solution, add lysozyme to form mixing after being well mixed
Solution；(3) mixed solution is slowly added to be mixed to form suspension in the colloidal sol.
5. the preparation method of rascal mango preservative according to claim 4, it is characterised in that：In step (3), the mixing
The speed that solution is added in the colloidal sol is 0.5~1mL/min.
6. a kind of rascal preservation method for mangoes, it is characterised in that rascal mango impregnated in the guarantor as described in claims 1 to 3
Dried after 5~10min in fresh dose.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108770924A (en) * | 2018-04-18 | 2018-11-09 | 许昌学院 | A method of with the fresh-keeping loquat of fruit of glossy privet leaching liquor |
| CN114698686A (en) * | 2022-04-18 | 2022-07-05 | 广西东鸣现代农业发展有限公司 | Natural apple preservative and preparation method thereof |
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| CN105053174A (en) * | 2015-09-17 | 2015-11-18 | 邯郸学院 | Preparation method and application of plant source complex preservative |
| CN105104513A (en) * | 2015-09-30 | 2015-12-02 | 邯郸学院 | Fruit and vegetable fresh-keeping agent containing memosene coneinnus extract and preparation method thereof |
| CN105360171A (en) * | 2015-10-12 | 2016-03-02 | 邯郸学院 | Preparation method and application of plant source bacteriostatic agent |
| CN105494618A (en) * | 2016-01-07 | 2016-04-20 | 石鸿娟 | Fruit and vegetable fresh-keeping agent containing oat grass extract and preparation method thereof |
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| CN105053174A (en) * | 2015-09-17 | 2015-11-18 | 邯郸学院 | Preparation method and application of plant source complex preservative |
| CN105104513A (en) * | 2015-09-30 | 2015-12-02 | 邯郸学院 | Fruit and vegetable fresh-keeping agent containing memosene coneinnus extract and preparation method thereof |
| CN105360171A (en) * | 2015-10-12 | 2016-03-02 | 邯郸学院 | Preparation method and application of plant source bacteriostatic agent |
| CN105494618A (en) * | 2016-01-07 | 2016-04-20 | 石鸿娟 | Fruit and vegetable fresh-keeping agent containing oat grass extract and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108770924A (en) * | 2018-04-18 | 2018-11-09 | 许昌学院 | A method of with the fresh-keeping loquat of fruit of glossy privet leaching liquor |
| CN114698686A (en) * | 2022-04-18 | 2022-07-05 | 广西东鸣现代农业发展有限公司 | Natural apple preservative and preparation method thereof |
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