CN106719871A - A kind of preparation method of control of leaf spot of corn soil conditioner - Google Patents
A kind of preparation method of control of leaf spot of corn soil conditioner Download PDFInfo
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- CN106719871A CN106719871A CN201611135245.0A CN201611135245A CN106719871A CN 106719871 A CN106719871 A CN 106719871A CN 201611135245 A CN201611135245 A CN 201611135245A CN 106719871 A CN106719871 A CN 106719871A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The invention discloses a kind of preparation method of control of leaf spot of corn soil conditioner, belong to soil conditioner field.The present invention first takes cicada pupa living will, its belly to be marked be put into after openning and soak in honey, solid medium is dried to obtain after immersion, Paecilomyces lilacinus seed liquor is uniformly sprayed at into solid culture primary surface carries out fermented and cultured, until being dried after covering with lavender fine hair bacterium, grinding, the cicada pupa powder of Paecilomyces lilacinus must be covered with, discarded Carapax Eriocheir sinensis are carried out into acid bubble and alkali bubble respectively again, rinse, with the cicada pupa powder for being covered with Paecilomyces lilacinus after drying, ammonium dihydrogen phosphate, Chinese milk vetch stalk, turf part tea seed cake mixing, can obtain control of leaf spot of corn soil conditioner, the soil conditioner that the present invention is prepared can significantly inhibit southern corn leaf blight mycelial growth, the incidence of disease of corn southern leaf blight is greatly lowered, compared with conventional chemical pesticide, crops will not be polluted, do not develop immunity to drugs, the preventing and treating time is long.
Description
Technical field
The invention discloses a kind of preparation method of control of leaf spot of corn soil conditioner, belong to soil conditioner neck
Domain.
Background technology
Maize diseases are the principal elements for restricting corn yield and quality.The corn caused by Fusarium oxysporum is small
Pinta is a kind of global Maize Leaf fungal disease.In recent years, development, the corn product of industrialization are planted with fresh edible maize
The renewal and the change of cropping system planted, corn southern leaf blight occur increasingly serious in China main maize area.Corn southern leaf blight exists
Whole corn growth stage can occur, occur most beneficial for disease especially under the warm humid conditions after milpa takes out hero,
It is popular.The general susceptible variety underproduction more than 10%, it is serious that the time underproduction 20%~80% occurs, or even ruin kind of a total crop failure.The disease has
Occur it is universal, spread expansion rate it is fast the features such as, developed into most important disease in Maize Production in China.
At present, preventing and treating of the China to corn southern leaf blight mainly uses chemical prevention and control method.Corn-growing regions are year after year using single
One Agro-chemicals control corn southern leaf blight, is easily caused the generation of field drug-fastness, increases prevention effect reduction, drug cost
Plus.How pharmaceutical preparations are developed, be current prevention and control corn southern leaf blight urgent need to solve the problem.
Compared with the chemical insecticide of import, it is suitable or close to kill nematode effect, and it is produced into for Paecilomyces lilacinus microbial inoculum
This is relatively low, and has aftereffect, and has the advantages that not pollute agricultural product, free from environmental pollution.Paecilomyces lilacinus class is environment-friendly to kill
The food and the sustainable development of Crop securify, agricultural researched and developed successfully for China of entomogenous fungi agent are respectively provided with significance, because
This, Paecilomyces lilacinus microbial inoculum has larger potentiality in the biological control of plant nematode.
The content of the invention
Present invention mainly solves technical problem：Prevent and treat beautiful using Single chemistry medicine year after year for current corn-growing regions
Rice helminthosporium maydis, are easily caused the generation of field drug-fastness, reduce prevention effect, and the lasting period shortens, and is unfavorable for holding for environment
The problem of supervention exhibition, there is provided a kind of method that utilization Paecilomyces lilacinus prepare soil conditioner control of leaf spot of corn, this
Invention first take cicada pupa living will, its belly to be marked be put into honey after openning and soaked, solid medium is dried to obtain after immersion, will be light
Purple Paecilomyces varioti seed liquor is uniformly sprayed at solid culture primary surface and carries out fermented and cultured, until being done after covering with lavender fine hair bacterium
Dry, grinding, must be covered with the cicada pupa powder of Paecilomyces lilacinus, then discarded Carapax Eriocheir sinensis are carried out into acid bubble and alkali bubble respectively, rinse,
With cicada pupa powder, ammonium dihydrogen phosphate, Chinese milk vetch stalk, the turf part tea seed cake mixing for being covered with Paecilomyces lilacinus after drying, you can
Control of leaf spot of corn soil conditioner is obtained, the soil conditioner that the present invention is prepared can significantly inhibit southern corn leaf blight
Mycelial growth, is greatly lowered the incidence of disease of corn southern leaf blight, compared with conventional chemical pesticide, will not pollute crops,
Do not develop immunity to drugs, the preventing and treating time is long, has broad prospect of application in agricultural use.
In order to solve the above-mentioned technical problem, the technical solution adopted in the present invention is：
（1）200~400g work cicada pupas are weighed, the openning of 1cm length is scratched in the belly of every cicada pupa, the cicada pupa after designated port is put
Enter in 600~800g honey and soak, taken out after 30~50h of immersion, the cicada pupa that will be covered with honey takes out, be put under the sun and be exposed to the sun 2
~4 days, collected after being exposed to the sun；
（2）The cicada pupa surface that 300~400mL Paecilomyces lilacinus seed liquor is uniformly sprayed at after above-mentioned being exposed to the sun is measured, is put after sprinkling
To enter be uniformly mixed under gnotobasis, the cicada pupa after stirring is put into glass container, and vessel port is sealed with gauze, be put into
Temperature is 28~30 DEG C, and humidity is to carry out fermented and cultured in the environment of 80~85%, the humidity after culture 2~4 days in adjusting ambient
It is 40~50%, continues fermented and cultured to growing lilac fine hair bacterium, and carries out cover on cicada pupa surface completely；
（3）The above-mentioned cicada pupa for covering with fine hair bacterium is collected, is put into vacuum drying chamber, it is vacuum dried 3 under the conditions of 30~35 DEG C~
5h, is ground 10~15min in addition mortar after drying, collection is covered with the cicada pupa powder of Paecilomyces lilacinus, standby；
（4）The discarded Carapax Eriocheir sinensis of 300~500g are weighed, surface is put into baking oven after cleaning, and 5 are dried under the conditions of 100~110 DEG C
~10h, is put into crushing in pulverizer after drying, cross 120~150 mesh sieves, crab shell powder is obtained, by crab shell powder in mass ratio 1:5
With the mixed in hydrochloric acid of mass fraction 20%, 5~7h is soaked after being uniformly mixed, filter residue is washed with deionized suction filtration after immersion
3~5 times, by filter residue after washing in mass ratio 1:5 mix with the sodium hydroxide solution of mass fraction 10%, are filtered after 3~5h of immersion,
Filter residue is obtained, is put into baking oven after being washed with clear water, 5~10h is dried under the conditions of 60~70 DEG C, obtain dried object；
（5）Count by weight, 50~70 parts of steps are chosen respectively（3）It is covered with cicada pupa powder, 25~30 of Paecilomyces lilacinus
The above-mentioned dried object of part, 3~5 parts of ammonium dihydrogen phosphates, 10~20 parts of Chinese milk vetch stalks, 5~10 parts of turfs, 10~15 parts of tea seed cakes, stir
Mix and place 8~10h at room temperature after being well mixed, packed after placement, you can obtain control of leaf spot of corn soil conditioner.
Application process of the invention：The control of leaf spot of corn soil conditioner that the present invention is prepared is sowed in corn
Tanaka, in the Maize at Seedling Stage stage, the amount of sowing is 150~200g/m2, when corn growth to the ear period stage, the amount of sowing be 280~
400g/m2, maturation is grown to naturally after sowing, the control rate to corn southern leaf blight reaches more than 95%, has to corn southern leaf blight
Preferable prevention effect, makes corn yield increasing 5~8%, with broad prospect of application.
The beneficial effects of the invention are as follows：
（1）The soil conditioner that the present invention is prepared can significantly inhibit southern corn leaf blight mycelial growth, be greatly lowered
The incidence of disease of corn southern leaf blight；
（2）The control of leaf spot of corn soil conditioner that the present invention is prepared will not pollute compared with conventional chemical pesticide
Crops, do not develop immunity to drugs, and the preventing and treating time is long, has broad prospect of application in agricultural use.
Specific embodiment
200~400g work cicada pupas are weighed, the openning of 1cm length is scratched in the belly of every cicada pupa, by the cicada pupa after designated port
It is put into 600~800g honey and soaks, taken out after 30~50h of immersion, the cicada pupa that will be covered with honey takes out, is put under the sun and is exposed to the sun
2~4 days, collected after being exposed to the sun；Measure the cicada pupa table that 300~400mL Paecilomyces lilacinus seed liquor is uniformly sprayed at after above-mentioned being exposed to the sun
Face, is put under gnotobasis after sprinkling and is uniformly mixed, and the cicada pupa after stirring is put into glass container, and sealed with gauze
Vessel port, is put into temperature for 28~30 DEG C, and humidity is to carry out fermented and cultured in the environment of 80~85%, and culture is adjusted after 2~4 days
Humidity in environment is 40~50%, continues fermented and cultured to growing lilac fine hair bacterium, and carries out cover in cicada pupa table completely
Face；The above-mentioned cicada pupa for covering with fine hair bacterium is collected, is put into vacuum drying chamber, 3~5h is vacuum dried under the conditions of 30~35 DEG C, done
10~15min is ground after dry in addition mortar, collection is covered with the cicada pupa powder of Paecilomyces lilacinus, standby；Weigh 300~
500g discarded Carapax Eriocheir sinensis, surface is put into baking oven after cleaning, and 5~10h is dried under the conditions of 100~110 DEG C, is put into after drying
Crushed in pulverizer, cross 120~150 mesh sieves, crab shell powder is obtained, by crab shell powder in mass ratio 1:5 with the hydrochloric acid of mass fraction 20%
Mixing, soaks 5~7h after being uniformly mixed, filter residue is washed with deionized 3~5 times suction filtration after immersion, will be filtered after washing
Slag in mass ratio 1:5 mix with the sodium hydroxide solution of mass fraction 10%, are filtered after 3~5h of immersion, obtain filter residue, are washed with clear water
After be put into baking oven, under the conditions of 60~70 DEG C dry 5~10h, obtain dried object；Count by weight, 50~70 are chosen respectively
Part is covered with the cicada pupa powder of Paecilomyces lilacinus, 25~30 parts of above-mentioned dried objects, 3~5 parts of ammonium dihydrogen phosphates, 10~20 parts of purple clouds
English stalk, 5~10 parts of turfs, 10~15 parts of tea seed cakes, place 8~10h at room temperature after being uniformly mixed, packed after placement,
Can obtain control of leaf spot of corn soil conditioner.
Example 1
200g work cicada pupas are weighed, the openning of 1cm length is scratched in the belly of every cicada pupa, the cicada pupa after designated port is put into 600g honeybees
Soaked in honey, taken out after immersion 30h, the cicada pupa that will be covered with honey takes out, and is put under the sun and is exposed to the sun 2 days, is collected after being exposed to the sun；Measure
300mL Paecilomyces lilacinus seed liquor is uniformly sprayed at the cicada pupa surface after above-mentioned being exposed to the sun, and stirring under gnotobasis is put into after sprinkling
It is well mixed, the cicada pupa after stirring is put into glass container, and vessel port is sealed with gauze, it is 28 DEG C, humidity to be put into temperature
To carry out fermented and cultured in the environment of 80%, the humidity after culture 2 days in adjusting ambient is 40%, continues fermented and cultured to growing
Lilac fine hair bacterium, and shoe is covered on cicada pupa surface completely；The above-mentioned cicada pupa for covering with fine hair bacterium is collected, vacuum drying chamber is put into
In, 3h is vacuum dried under the conditions of 30 DEG C, 10min is ground after drying in addition mortar, collection is covered with Paecilomyces lilacinus
Cicada pupa powder, it is standby；The discarded Carapax Eriocheir sinensis of 300g are weighed, surface is put into baking oven after cleaning, and is dried under the conditions of 100 DEG C
5h, is put into crushing in pulverizer after drying, cross 120 mesh sieves, crab shell powder is obtained, by crab shell powder in mass ratio 1:5 divide with quality
Several 20% mixed in hydrochloric acid, soak 5h after being uniformly mixed, suction filtration after immersion, and filter residue is washed with deionized 3 times, will wash
Filter residue in mass ratio 1 afterwards:5 mix with the sodium hydroxide solution of mass fraction 10%, are filtered after immersion 3h, obtain filter residue, are washed with clear water
After be put into baking oven, dry 5h under the conditions of 60 DEG C, obtain dried object；Count by weight, choosing 50 parts respectively is covered with pale purple plan
The cicada pupa powder of Penicillium notatum, 25 parts of above-mentioned dried objects, 3 parts of ammonium dihydrogen phosphates, 10 parts of Chinese milk vetch stalks, 5 parts of turfs, 10 parts of tea
The dregs of rice, place 8h at room temperature after being uniformly mixed, packed after placement, you can obtain control of leaf spot of corn soil conditioner.
The control of leaf spot of corn soil conditioner that the present invention is prepared is sowed in corn field, in Maize at Seedling Stage rank
Section, the amount of sowing is 150g/m2, when corn growth to the ear period stage, the amount of sowing is 280g/m2, grown into naturally after sowing
Ripe, the control rate to corn southern leaf blight reaches 96%, has preferable prevention effect to corn southern leaf blight, makes corn yield increasing 5%, tool
There is broad prospect of application.
Example 2
300g work cicada pupas are weighed, the openning of 1cm length is scratched in the belly of every cicada pupa, the cicada pupa after designated port is put into 700g honeybees
Soaked in honey, taken out after immersion 40h, the cicada pupa that will be covered with honey takes out, and is put under the sun and is exposed to the sun 3 days, is collected after being exposed to the sun；Measure
350mL Paecilomyces lilacinus seed liquor is uniformly sprayed at the cicada pupa surface after above-mentioned being exposed to the sun, and stirring under gnotobasis is put into after sprinkling
It is well mixed, the cicada pupa after stirring is put into glass container, and vessel port is sealed with gauze, it is 29 DEG C, humidity to be put into temperature
To carry out fermented and cultured in the environment of 83%, the humidity after culture 3 days in adjusting ambient is 45%, continues fermented and cultured to growing
Lilac fine hair bacterium, and shoe is covered on cicada pupa surface completely；The above-mentioned cicada pupa for covering with fine hair bacterium is collected, vacuum drying chamber is put into
In, 4h is vacuum dried under the conditions of 33 DEG C, 13min is ground after drying in addition mortar, collection is covered with Paecilomyces lilacinus
Cicada pupa powder, it is standby；The discarded Carapax Eriocheir sinensis of 400g are weighed, surface is put into baking oven after cleaning, and is dried under the conditions of 105 DEG C
8h, is put into crushing in pulverizer after drying, cross 140 mesh sieves, crab shell powder is obtained, by crab shell powder in mass ratio 1:5 divide with quality
Several 20% mixed in hydrochloric acid, soak 6h after being uniformly mixed, suction filtration after immersion, and filter residue is washed with deionized 4 times, will wash
Filter residue in mass ratio 1 afterwards:5 mix with the sodium hydroxide solution of mass fraction 10%, are filtered after immersion 4h, obtain filter residue, are washed with clear water
After be put into baking oven, dry 8h under the conditions of 65 DEG C, obtain dried object；Count by weight, choosing 60 parts respectively is covered with pale purple plan
The cicada pupa powder of Penicillium notatum, 27 parts of above-mentioned dried objects, 4 parts of ammonium dihydrogen phosphates, 15 parts of Chinese milk vetch stalks, 8 parts of turfs, 13 parts of tea
The dregs of rice, place 9h at room temperature after being uniformly mixed, packed after placement, you can obtain control of leaf spot of corn soil conditioner.
The control of leaf spot of corn soil conditioner that the present invention is prepared is sowed in corn field, in Maize at Seedling Stage rank
Section, the amount of sowing is 170g/m2, when corn growth to the ear period stage, the amount of sowing is 350g/m2, grown into naturally after sowing
Ripe, the control rate to corn southern leaf blight reaches 98%, has preferable prevention effect to corn southern leaf blight, makes corn yield increasing 6%, tool
There is broad prospect of application.
Example 3
400g work cicada pupas are weighed, the openning of 1cm length is scratched in the belly of every cicada pupa, the cicada pupa after designated port is put into 800g honeybees
Soaked in honey, taken out after immersion 50h, the cicada pupa that will be covered with honey takes out, and is put under the sun and is exposed to the sun 4 days, is collected after being exposed to the sun；Measure
400mL Paecilomyces lilacinus seed liquor is uniformly sprayed at the cicada pupa surface after above-mentioned being exposed to the sun, and stirring under gnotobasis is put into after sprinkling
It is well mixed, the cicada pupa after stirring is put into glass container, and vessel port is sealed with gauze, it is 30 DEG C, humidity to be put into temperature
To carry out fermented and cultured in the environment of 85%, the humidity after culture 4 days in adjusting ambient is 50%, continues fermented and cultured to growing
Lilac fine hair bacterium, and shoe is covered on cicada pupa surface completely；The above-mentioned cicada pupa for covering with fine hair bacterium is collected, vacuum drying chamber is put into
In, 5h is vacuum dried under the conditions of 35 DEG C, 15min is ground after drying in addition mortar, collection is covered with Paecilomyces lilacinus
Cicada pupa powder, it is standby；The discarded Carapax Eriocheir sinensis of 500g are weighed, surface is put into baking oven after cleaning, and is dried under the conditions of 110 DEG C
10h, is put into crushing in pulverizer after drying, cross 150 mesh sieves, crab shell powder is obtained, by crab shell powder in mass ratio 1:5 divide with quality
Several 20% mixed in hydrochloric acid, soak 7h after being uniformly mixed, suction filtration after immersion, and filter residue is washed with deionized 5 times, will wash
Filter residue in mass ratio 1 afterwards:5 mix with the sodium hydroxide solution of mass fraction 10%, are filtered after immersion 5h, obtain filter residue, are washed with clear water
After be put into baking oven, dry 10h under the conditions of 70 DEG C, obtain dried object；Count by weight, choosing 70 parts respectively is covered with pale purple plan
The cicada pupa powder of Penicillium notatum, 30 parts of above-mentioned dried objects, 5 parts of ammonium dihydrogen phosphates, 20 parts of Chinese milk vetch stalks, 10 parts of turfs, 15 parts of tea
The dregs of rice, place 8~10h at room temperature after being uniformly mixed, packed after placement, you can obtain control of leaf spot of corn soil improvement
Agent.
The control of leaf spot of corn soil conditioner that the present invention is prepared is sowed in corn field, in Maize at Seedling Stage rank
Section, the amount of sowing is 200g/m2, when corn growth to the ear period stage, the amount of sowing is 400g/m2, grown into naturally after sowing
Ripe, the control rate to corn southern leaf blight reaches 99%, has preferable prevention effect to corn southern leaf blight, makes corn yield increasing 8%, tool
There is broad prospect of application.
Claims (1)
1. a kind of preparation method of control of leaf spot of corn soil conditioner, it is characterised in that specific preparation process is：
（1）200~400g work cicada pupas are weighed, the openning of 1cm length is scratched in the belly of every cicada pupa, the cicada pupa after designated port is put
Enter in 600~800g honey and soak, taken out after 30~50h of immersion, the cicada pupa that will be covered with honey takes out, be put under the sun and be exposed to the sun 2
~4 days, collected after being exposed to the sun；
（2）The cicada pupa surface that 300~400mL Paecilomyces lilacinus seed liquor is uniformly sprayed at after above-mentioned being exposed to the sun is measured, is put after sprinkling
To enter be uniformly mixed under gnotobasis, the cicada pupa after stirring is put into glass container, and vessel port is sealed with gauze, be put into
Temperature is 28~30 DEG C, and humidity is to carry out fermented and cultured in the environment of 80~85%, the humidity after culture 2~4 days in adjusting ambient
It is 40~50%, continues fermented and cultured to growing lilac fine hair bacterium, and carries out cover on cicada pupa surface completely；
（3）The above-mentioned cicada pupa for covering with fine hair bacterium is collected, is put into vacuum drying chamber, it is vacuum dried 3 under the conditions of 30~35 DEG C~
5h, is ground 10~15min in addition mortar after drying, collection is covered with the cicada pupa powder of Paecilomyces lilacinus, standby；
（4）The discarded Carapax Eriocheir sinensis of 300~500g are weighed, surface is put into baking oven after cleaning, and 5 are dried under the conditions of 100~110 DEG C
~10h, is put into crushing in pulverizer after drying, cross 120~150 mesh sieves, crab shell powder is obtained, by crab shell powder in mass ratio 1:5
With the mixed in hydrochloric acid of mass fraction 20%, 5~7h is soaked after being uniformly mixed, filter residue is washed with deionized suction filtration after immersion
3~5 times, by filter residue after washing in mass ratio 1:5 mix with the sodium hydroxide solution of mass fraction 10%, are filtered after 3~5h of immersion,
Filter residue is obtained, is put into baking oven after being washed with clear water, 5~10h is dried under the conditions of 60~70 DEG C, obtain dried object；
（5）Count by weight, 50~70 parts of steps are chosen respectively（3）It is covered with cicada pupa powder, 25~30 of Paecilomyces lilacinus
The above-mentioned dried object of part, 3~5 parts of ammonium dihydrogen phosphates, 10~20 parts of Chinese milk vetch stalks, 5~10 parts of turfs, 10~15 parts of tea seed cakes, stir
Mix and place 8~10h at room temperature after being well mixed, packed after placement, you can obtain control of leaf spot of corn soil conditioner.
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| CN101473855A (en) * | 2009-02-16 | 2009-07-08 | 何松庆 | Propagating agent for preventing fungus in field |
| CN101560270A (en) * | 2009-06-09 | 2009-10-21 | 杨甫进 | Energy-saving and emission-reducing chitin production method |
| CN103789211A (en) * | 2012-10-31 | 2014-05-14 | 国家海洋局第三海洋研究所 | Paecilomyces lilacinus A65 coming from deep-sea sediment and having anti-pathomycete activity for tea leaves |
| CN104719345A (en) * | 2015-03-31 | 2015-06-24 | 青州伟格生物科技有限公司 | Preparation method of biological preparation for preventing and controlling vegetable nematode disease |
| CN105316243A (en) * | 2015-11-23 | 2016-02-10 | 鲁东大学 | Preparing method and application of composite biological agent for agricultural root-knot nematodes |
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