CN106718947A - One kind promotes the value-added tissue culture method of banana callus - Google Patents
One kind promotes the value-added tissue culture method of banana callus Download PDFInfo
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- CN106718947A CN106718947A CN201710171828.7A CN201710171828A CN106718947A CN 106718947 A CN106718947 A CN 106718947A CN 201710171828 A CN201710171828 A CN 201710171828A CN 106718947 A CN106718947 A CN 106718947A
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- callus
- tissue culture
- concentration
- light source
- banana
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention provides a kind of promotion value-added tissue culture method of banana callus, its culture medium based on MS culture mediums, have adjusted in culture medium hormone prescription and with the addition of rare earth element, and addition strengthens colour light source in the middle of increment culture, the value added on products of banana callus is drastically increased, the present invention has the advantages that value-added coefficient is high.
Description
Technical field
The invention belongs to Banana Tissue culture technique field, more particularly to a kind of promotion value-added tissue culture of banana callus
Method.
Background technology
Banana（Scientific name：Musa nana Lour.）Musaceae banana, refers to its fruit again.Torrid areas plants extensively
Training is edible.Banana flavor is fragrant, rich in nutrition, can harvest throughout the year, also paid attention to very much in Temperate Region in China.Plant is large-scale draft, from root
Shape stem is sent, and the false bar of (10~20 chi) high 3~6 meters is formed by leaf sheath bottom；Leaf Long Circle to ellipse, have up to 3
~3.5 meters (10~11.5 chi), (26 cun) wide 65 centimeters, 10~20 pieces of fasciation stem tops.Spike is sagging[1], by false bar top
End is extracted out, spends majority, faint yellow；Infructescence is curved to hang down, as a result 10~20 string, about 50~150.It is withered after plant result, by root shape
The sucker that stem length goes out continues to breed, and each stock can live for many years.Originate in the Asia southeast：The ground such as Taiwan, Hainan, Guangdong, Guangxi
Qu Junyou is cultivated.Current banana is a kind of very popular fruit, is also the main food of some areas, and possessing very big market needs
The amount of asking, the research that quick breeding is carried out to banana has certain necessity, can not only solve huge market demand, also can
Excellent banana variety is preserved to a certain extent, and tissue cultures are one of banana rapid propagation methods, but current perfume (or spice)
Any of several broadleaf plants tissue cultures value adding method growth factor is relatively low.
Rare earth element is 17 kinds of general designations of special element, and it gains the name because Sweden scientist is in Extraction of rare earth element
When apply rare earth compound, so rare earth element of gaining the name.But the title that rare earth is left over by history to get off, rare earth is from 18 generation
End beginning of recording finds that water insoluble soild oxide was often called soil by people at that time successively, for example, aluminum oxide is referred to as into " pottery
Soil ", calcium oxide is referred to as " alkaline earth " etc..Rare earth is usually to be separated with oxidation state, at that time than sparser, thus is gained the name
It is rare earth（Rare Earth, abbreviation RE or R）, but by substantial amounts of scientific research, rare earth is with the addition of in the middle of tissue cultures
Element can greatly improve the quality of tissue cultures.
Cerium is periodic system Ι Ι Ι races subgroup lanthanide series, a kind of rare earth element.Atomic number 58.Stable isotope：136、
138、140、142.Grey metal, there is malleability.Density：Quadratic crystal 6.9, cubic crystal 6.7.799 DEG C of fusing point, boiling point 3426
℃.Cerium is a kind of silver-gray active metal, and powder easy spontaneous combustion in atmosphere is soluble in acid.The title of cerium derives from asteroid
The English name of ceres.Content of the cerium in the earth's crust about 0.0046%, is abundance highest in rare earth element.
The content of the invention
Above mentioned problem is had based on prior art, the present invention provides a kind of promotion banana callus value-added tissue culture side
Method, its culture medium based on MS culture mediums have adjusted in culture medium hormone prescription and with the addition of rare earth element, and trained in increment
Addition enhancing colour light source in the middle of supporting, drastically increases the value added on products of banana callus, and the present invention has value-added coefficient high
Advantage.
One kind promotes the value-added tissue culture method of banana callus, and it is comprised the following steps：
Step S10, culture medium is prepared by formula as below：The culture medium based on MS culture mediums, the concentration of chaff adenine phosphate is
0.4-0.6mg/L、NAA（Methyl α-naphthyl acetate）Concentration be 2-3 mg/L, the concentration of activated carbon be 0.1-0.15 mg/L, cerous nitrate
Concentration is 2-4g/L, and the concentration of vitamin is 0.2-0.4mg/L, and the concentration of agar powder is 9-11g/L, the concentration of white granulated sugar is 6-
8g/L；
Step S20, normal and health the banana callus of growth selection, by the improper portion of callus in superclean bench
Cutting is removed, and it is 0.4-0.6cm that callus scalpel is cut into volume3Size, and be inoculated into equipped with step S10 prepare
In the tissue culture bottle of good culture medium, and it is transferred in tissue culture room；
Step S30,27 DEG C~31 DEG C are adjusted to by the environment temperature in tissue culture room, and light source and green is strengthened using natural light, blue light
Light enhancing light source irradiation callus, light application time is illumination 16 hours/dark 8 hours.
Wherein, step S11 is also included in described step S10,121 DEG C of 20 points of high-temp steam sterilizing is carried out to culture medium
Clock.
Wherein, the enhancing light source in described step S30 is LED, and green enhancing light source is green LED lamp, and blueness increases
Intense light source blue LED lamp.
Wherein, the green light source in described step S50 is the green glow that wave band is 550nm-580nm, and blue-light source is ripple
Section is the blue light of 450nm-470nm.
Specific embodiment
With reference to specific embodiment, the invention will be further described.
One kind promotes the value-added tissue culture method of banana callus, and it is comprised the following steps：
Step S10, culture medium is prepared by formula as below：The culture medium based on MS culture mediums, the concentration of chaff adenine phosphate is
0.4-0.6mg/L、NAA（Methyl α-naphthyl acetate）Concentration be 2-3 mg/L, the concentration of activated carbon be 0.1-0.15 mg/L, cerous nitrate
Concentration is 2-4g/L, and the concentration of vitamin is 0.2-0.4mg/L, and the concentration of agar powder is 9-11g/L, the concentration of white granulated sugar is 6-
8g/L, then performs step S11,121 DEG C of high-temp steam sterilizing is carried out to culture medium 20 minutes；
Step S20, normal and health the banana callus of growth selection, by the improper portion of callus in superclean bench
Cutting is removed, and it is 0.4-0.6cm that callus scalpel is cut into volume3Size, and be inoculated into equipped with step S10 prepare
In the tissue culture bottle of good culture medium, and it is transferred in tissue culture room；
Step S30,27 DEG C~31 DEG C are adjusted to by the environment temperature in tissue culture room, the use of natural light, wave band are 450nm-470nm
Blue light enhancing light source and wave band for 550nm-580nm green glow enhancing light source irradiation callus, light application time be illumination 16
Hour/dark 8 hours；
The banana callus of well cutting is inoculated into the group provided using the present invention in the culture medium that step S10 is prepared
Cultural method is knitted, the value-added coefficient that banana callus is calculated after 20 days is 4.2, higher than conventional culture methods, value-added system number
Computational methods be culture after callus quality divided by culture before callus quality.
Embodiment described above only expresses one embodiment of the present invention, and its description is more specific and detailed, but can not
Therefore it is interpreted as the limitation to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection model of the invention
Enclose.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (4)
1. it is a kind of to promote the value-added tissue culture method of banana callus, it is characterised in that it is comprised the following steps：
Step S10, culture medium is prepared by formula as below：The culture medium based on MS culture mediums, the concentration of chaff adenine phosphate is
0.4-0.6mg/L、NAA（Methyl α-naphthyl acetate）Concentration be 2-3 mg/L, the concentration of activated carbon be 0.1-0.15 mg/L, cerous nitrate
Concentration is 2-4g/L, and the concentration of vitamin is 0.2-0.4mg/L, and the concentration of agar powder is 9-11g/L, the concentration of white granulated sugar is 6-
8g/L；
Step S20, normal and health the banana callus of growth selection, by the improper portion of callus in superclean bench
Cutting is removed, and it is 0.4-0.6cm that callus scalpel is cut into volume3Size, and be inoculated into equipped with step S10 prepare
In the tissue culture bottle of good culture medium, and it is transferred in tissue culture room；
Step S30,27 DEG C~31 DEG C are adjusted to by the environment temperature in tissue culture room, and light source and green is strengthened using natural light, blue light
Light enhancing light source irradiation callus, light application time is illumination 16 hours/dark 8 hours.
2. one kind according to claim 1 promotes the value-added tissue culture method of banana callus, it is characterised in that described
Also include step S11 in step S10,121 DEG C of high-temp steam sterilizing is carried out to culture medium 20 minutes.
3. one kind according to claim 1 promotes the value-added tissue culture method of banana callus, it is characterised in that described
Enhancing light source in step S30 is LED, and green enhancing light source is green LED lamp, blueness enhancing light source blue LED lamp.
4. one kind according to claim 3 promotes the value-added tissue culture method of banana callus, it is characterised in that described
Green light source in step S50 is the green glow that wave band is 550nm-580nm, and blue-light source is the indigo plant that wave band is 450nm-470nm
Light.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710171828.7A CN106718947A (en) | 2017-03-22 | 2017-03-22 | One kind promotes the value-added tissue culture method of banana callus |
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|---|---|---|---|
| CN201710171828.7A CN106718947A (en) | 2017-03-22 | 2017-03-22 | One kind promotes the value-added tissue culture method of banana callus |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1186116A (en) * | 1996-12-23 | 1998-07-01 | 中国科学院遗传研究所 | Tissue culture for banana |
| CN101518208A (en) * | 2009-03-23 | 2009-09-02 | 中国热带农业科学院热带生物技术研究所 | High-efficiency method for induction and successive transfer proliferation regeneration of banana embryogenic calluses |
| CN102283109A (en) * | 2010-06-17 | 2011-12-21 | 上海上房园艺有限公司 | Breeding method of new variety of banana |
| CN104871975A (en) * | 2015-05-27 | 2015-09-02 | 杨树东 | Banana tissue culture propagation method |
-
2017
- 2017-03-22 CN CN201710171828.7A patent/CN106718947A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1186116A (en) * | 1996-12-23 | 1998-07-01 | 中国科学院遗传研究所 | Tissue culture for banana |
| CN101518208A (en) * | 2009-03-23 | 2009-09-02 | 中国热带农业科学院热带生物技术研究所 | High-efficiency method for induction and successive transfer proliferation regeneration of banana embryogenic calluses |
| CN102283109A (en) * | 2010-06-17 | 2011-12-21 | 上海上房园艺有限公司 | Breeding method of new variety of banana |
| CN104871975A (en) * | 2015-05-27 | 2015-09-02 | 杨树东 | Banana tissue culture propagation method |
Non-Patent Citations (6)
| Title |
|---|
| 何忠效等: "《生物技术概论》", 30 June 2006, 北京师范大学出版社 * |
| 刘雪红等: "香蕉薄切片愈伤组织再生体系的建立研究", 《福建林业科技》 * |
| 张秀枝等: "香蕉、大蕉、粉蕉胚性愈伤组织的诱导", 《农业与技术》 * |
| 王水琦: "《植物组织培养》", 31 March 2007, 中国轻工业出版社 * |
| 董春英等: "稀土元素在组织培养中的作用研究进展", 《湖南林业科技》 * |
| 黄霞等: "果用香蕉薄片外植体植株再生的研究", 《园艺学报》 * |
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Application publication date: 20170531 |