CN106718873A - A kind of method of good blue Rapid Propagation of Lilium - Google Patents
A kind of method of good blue Rapid Propagation of Lilium Download PDFInfo
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- CN106718873A CN106718873A CN201611000512.3A CN201611000512A CN106718873A CN 106718873 A CN106718873 A CN 106718873A CN 201611000512 A CN201611000512 A CN 201611000512A CN 106718873 A CN106718873 A CN 106718873A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The present invention relates to a kind of method of good blue Rapid Propagation of Lilium, belong to plant tissue culture technical field.The method is accessed in inducing culture by the good blue lily explant for processing sterilization, induce adventitious bud, then cutting adventitious bud carries out squamous subculture, squamous subculture repeatedly after obtaining the required adventitious bud quantity with stem disk, cuts during stem disk is forwarded to stem tuber culture medium and cultivates, and is directly transplanted on seedbed after length to the stem tuber of 1 ~ 1.5cm of diameter, germination and emergence after 40 ~ 60d, a large amount of good orchid lily seedlings are can obtain, enters kind of rate up to 90%, transplanting survival rate is more than 95%.This method solve the key technology that good orchid lily sterile system is set up; pass through hormone, nutrition, the comprehensive adjustment of culture environment simultaneously; solve that good orchid lily breeding coefficient is low, the problem that the production cycle is long; reach the purpose that kind of rate is high, growth rate is fast, production technology is stable, realize the scale of good orchid lily, standardization, factorial praluction.
Description
Technical field
The invention belongs to plant tissue culture technical field, and in particular to a kind of method of good blue Rapid Propagation of Lilium.
Background technology
Good orchid lily (GloriosasuperbaLinn) good Cymbidium the climbing up by holding on to property herbaceous plant of category Liliaceae, originates in me
Yunnan Province of state south, Hainan Province and Asia Africa torrid areas, below Yunnan Province of China Xishuangbanna height above sea level 1200m has wild fragmentary
Distribution.Flower appearance is peculiar, beautiful in colour and graceful just as burned flame；Pattern change is various, and the florescence is more long, is a kind of great
The herbage flower of ornamental value.Because being rich in colchicin, its stem tuber is one of desirable feedstock of production colchicin.Good orchid lily
Modes of reproduction have seminal propagation, stem tuber vegetative propagation and tissue culture propagation.Because the seminal propagation for praising orchid lily is difficult and growth
Slowly, need just bloom for 3 ~ 4 years, vegetative propagation is generally carried out using its underground stem tuber.However, the breeding coefficient of good orchid lily is non-
Often low, every plant can be only formed 1 stem tuber, and each stem tuber only has 2 buds, seriously constrain the large-scale production of good orchid lily.Adopt
The tissue-cultured seedling produced with conventional tissue culture propagation technology, then it is low to be faced with transition cultivating survival rate, and late growing stage is slow, culture week
The problems such as phase is long.Also due to good orchid lily is the good Cymbidium of Liliaceae, and common lily cultivar is Liliaceae lilium, due to not
It is a plant for category, its proliferation bias is very big, without reference value.Therefore it is difficult to meet good orchid with conventional propagation method
The scale of lily is quickly produced.
The content of the invention
For good blue lily seed breeding difficulty, stem tuber breeding coefficient is low, tissue culture propagation transplanting late growing stage is slow, the time
Long the problems such as, the present invention provides a kind of method of good blue Rapid Propagation of Lilium, by improving production procedure and culture medium prescription, carries
Gao Jialan lily breeding coefficients, quickly produce a large amount of high-quality stem tubers, to meet the large-scale production needs of high quality seedling.
To achieve the above object, the technical solution adopted by the present invention is as follows：
A kind of method of good blue Rapid Propagation of Lilium, comprises the following steps：
A, the selection of explant and sterilizing：The stem apex and/or axillary bud chosen robust growth and do not form bud are explant, are peelled off
Outer blade, is cut into the segment of a length of 1.5 ~ 2.0cm, cleans up and sterilizes, then extremely residual without disinfectant with rinsed with sterile water
Stay；
B, adventitious bud induction culture：To be inoculated in adventitious bud induction culture base by the segment after step A sterilization, in training
Support temperature be 27 ± 2 DEG C, intensity of illumination be 2000~3000lx, day light application time be 10~12h/d under conditions of, Fiber differentiation
20 ~ 40d differentiates 0.5 ~ 1.5cm adventitious buds long until explant is sprouted；
Described adventitious bud induction culture base is：MS culture mediums+6.0mg/L ~ 8.0mg/L 6-benzyl aminopurines+0.01mg/L ~
0.05mg/L methyl α-naphthyl acetates+6.5g/L ~ 7.0g/L agar+30g/L sucrose, pH5.8 ~ 6.0；
C, adventitious bud shoot proliferation culture：The adventitious bud of step B is aseptically cut and is placed in proliferated culture medium, in training
Support temperature be 27 ± 2 DEG C, intensity of illumination be 2000~3000lx, day light application time be 10~12h/d under conditions of carry out subculture
Culture, obtains the adventitious bud with stem disk；
Described proliferated culture medium MS culture mediums+4.0mg/L ~ 5.0mg/L 6-benzyl aminopurines+0.05mg/L ~ 0.1mg/L naphthalenes
Acetic acid+agar 6.5g/L ~ 7.0g/L+ sucrose 30g/L, pH5.8 ~ 6.0；
D, stem tuber culture：The adventitious bud with stem disk that step C is obtained aseptically is cut into adventitious bud and two, stem disk
Point, the adventitious bud for cutting is returned and is seeded in the proliferated culture medium of step C, and squamous subculture is carried out again；And the stem disk for cutting goes
Except root, stem, Ye Hou, it is divided into 2 ~ 4 pieces and is seeded in stem tuber culture medium, be 27 ± 2 DEG C in cultivation temperature, intensity of illumination is
2000~3000lx, day light application time be 10~12h/d under conditions of carry out stem tuber 50 ~ 60d of culture, treat that stem tuber is long to diameter
Planted under bottle outlet by 1.0 ~ 1.5cm；
Described stem tuber culture medium is：MS culture mediums+1.0mg/L ~ 1.5mg/L 6-benzyl aminopurines+0.1mg/L ~ 0.5mg/L
Methyl α-naphthyl acetate+6.5g/L ~ 7.0g/L agar+60g/L sucrose, pH5.8 ~ 6.0；
E, transplanting：The stem tuber of D step bottle outlets is routinely cleaned the culture medium on stem tuber, it is 0.1~0.2% to be put into mass concentration
Carbendazim solution in sterilize 1~2min after, transplant to mass ratio be laterite：In the nutrient matrix of leaf mould=1 ︰ 1, need 40% ~
45% shade density, is routinely watered, fertilizing management, germination and emergence after 40 ~ 60d of growth, you can obtain good orchid lily kind
Seedling.
It is further preferred that the specific method of the cleaning and sterilization described in step A is added with detergent with conventional
Water cleaning after, sterilize 7 ~ 10min in the mercuric chloride solution that mass concentration is 0.1%, then in the hypochlorous acid that mass concentration is 0.3%
Sterilize 3 ~ 5min in sodium solution, then with rinsed with sterile water 3 ~ 4 times.
It is further preferred that described subculture cycle is 20 ~ 30d, in the generation of subculture 3 ~ 5, can obtain and largely carry stem disk
Adventitious bud.
It is further preferred that in step D, stem disk removal root, stem, the Ye Hou for cutting are divided into 2 ~ 4 pieces, every piece
Particle diameter is 0.3 ~ 0.5c
Compared with prior art, its advantage is the present invention：
1st, compared with seminal propagation technology, present invention breeding is easy and shortens growth cycle.Good orchid lily seed breeding is difficult,
Its kind of skin is harder, thickness, and seed germination rate is low, emerges irregular, and seedling-growing time is long, it is necessary to 3 ~ 4 years can just bloom.And it is of the invention
Breeding is easy, and breeding coefficient is high to enter kind of rate up to 90% up to 6 ~ 8 times, and transplanting survival rate is more than 95%.
2nd, compared with existing conventional tissue culture propagation method, bottle outlet transplanting survival rate of the invention is high, it is easier to maintenance pipe
Reason.At present, existing conventional tissue culture propagation method is, by adventitious bud inducing, then to carry out adventitious bud proliferation so as to obtain tissue culture
Seedling, breeding coefficient is up to 4 ~ 5 times, but the survival rate of transition cultivating is low, and only 60% or so, and late growing stage speed is slow.The present invention
By adventitious bud inducing, propagation, tubercle is then produced by adventitious bud inducing, by obtaining about 1.0 after 50 ~ 60d cultures ~
The stem tuber of 1.5cm, survival rate is high after bottle outlet is transplanted, up to 95%；Late growing stage speed is fast, but also can grow 3 ~ 5 buds,
Reproductive efficiency is substantially increased, remarkable in economical benefits increases, while being easy to cultivation management.
3rd, the inventive method establishes adventitious bud inducing, and the good blue lily for then producing stem tuber by adventitious bud inducing breeds
System, at the same by hormone, nutrition, culture environment comprehensive adjustment, solve that good orchid lily breeding coefficient is low, the production cycle is long
Problem, reached that kind of rate is high, growth rate fast, the purpose of production technology stabilization realizes the scale of good orchid lily, standard
Change, factorial praluction.
Specific embodiment
With reference to embodiment, the present invention is described in further detail.
It will be understood to those of skill in the art that the following example is merely to illustrate the present invention, and should not be regarded as limiting this hair
Bright scope.In the examples where no specific technique or condition is specified, according to the technology or condition described by document in the art
Or carried out according to product description.Material therefor, reagent or the unreceipted production firm person of instrument, being can be obtained by purchase
The conventional products for obtaining.
Embodiment 1
A. the selection of explant and sterilizing：The stem apex and axillary bud chosen robust growth and do not form bud are explant, are peelled off outer
Layer blade, is cut into the segment of 1.5 ~ 2.0cm, is 0.1% in mass concentration after being cleaned with the conventional water added with appropriate detergent
Mercuric chloride solution in sterilize 7min, then the 5min that sterilized in the liquor natrii hypochloritis that mass concentration is 0.3%, then use sterilized water
Rinsing 4 times, then each 2min is blotted with aseptic filter paper, standby；
B, adventitious bud induction culture：Aseptically, following induction training will be inoculated in by the segment after step A sterilization
In foster base：MS+6- benayl aminopurines (6-BA) 6.0mg/L+ methyl α-naphthyl acetates（NAA）The g/L+ sucrose 30g/ of 0.01mg/L+ agar 6.5
L, pH=5.8, cultivation temperature be 27 DEG C, intensity of illumination be 2000~3000lx, light application time be 10h/d under conditions of, induction
Culture 40d differentiates 0.5 ~ 1.5cm adventitious buds until explant is sprouted；
C, adventitious bud shoot proliferation culture：The adventitious bud of step B is aseptically cut and is placed in following proliferated culture medium：
MS+6- benayl aminopurines (6-BA) 4.0mg/L+ methyl α-naphthyl acetates（NAA）0.05mg/L+ agar 6.5 g/L+ sucrose 30g/L, pH=
5.8, cultivation temperature be 27 DEG C, intensity of illumination be 2000~3000lx, day light application time be 10h/d under conditions of carry out subculture
Culture, subculture cycle is 30d, in the generation of subculture 3, obtains the substantial amounts of adventitious bud with stem disk；
D, stem tuber culture：The adventitious bud with stem disk that step C is obtained aseptically is cut into adventitious bud and two, stem disk
Point, the adventitious bud for cutting is returned and is seeded in the proliferated culture medium of step C, and squamous subculture is carried out again；And the stem disk for cutting goes
Except root, stem, Ye Hou, 2 ~ 4 pieces are divided into（Every piece of particle diameter is 0.3 ~ 0.5cm sizes）It is seeded in following stem tuber culture medium：
MS+6- benayl aminopurines (6-BA) 1.0mg/L+ methyl α-naphthyl acetates（NAA）0.1mg/L+ agar 6.5 g/L+ sucrose 60g/L, pH=5.8,
Cultivation temperature be 27 DEG C, intensity of illumination be 2000~3000lx, day light application time be 10h/d under conditions of carry out stem tuber culture
60d, treats that stem tuber is long and is planted to bottle outlet by diameter about 1.0cm.
E, transplanting：The stem tuber of D step bottle outlets is taken out, the culture medium on stem tuber is routinely cleaned, being put into mass concentration is
Sterilized in 0.1~0.2% carbendazim solution after 1~2min, it is laterite to transplant to mass ratio：The nutrient matrix of leaf mould=1 ︰ 1
In, 40% ~ 45% shade density is needed, routinely watered, fertilizing management, germination and emergence after 40 ~ 60d of growth, you can praised
Blue lily seedling.
Embodiment 2
A. the selection of explant and sterilizing：The stem apex and axillary bud chosen robust growth and do not form bud are explant, are peelled off outer
Layer blade, is cut into the segment of 1.5 ~ 2.0cm, is 0.1% in mass concentration after being cleaned with the conventional water added with appropriate detergent
Mercuric chloride solution in sterilize 8min, then the 4min that sterilized in the liquor natrii hypochloritis that mass concentration is 0.3%, then use sterilized water
Rinsing 4 times, then each 2min is blotted with aseptic filter paper, standby；
B, adventitious bud induction culture：Aseptically, following induction training will be inoculated in by the segment after step A sterilization
In foster base：MS+6- benayl aminopurines (6-BA) 7.0mg/L+ methyl α-naphthyl acetates（NAA）The g/L+ sucrose 30g/ of 0.03mg/L+ agar 7.0
L, pH=5.8, cultivation temperature be 27 DEG C, intensity of illumination be 2000~3000lx, light application time be 11h/d under conditions of, induction
Culture 30d differentiates 0.5 ~ 1.5cm adventitious buds until explant is sprouted；
C, adventitious bud shoot proliferation culture：The adventitious bud of step B is aseptically cut and is placed in following proliferated culture medium：
MS+6- benayl aminopurines (6-BA) 4.5mg/L+ methyl α-naphthyl acetates（NAA）0.08mg/L+ agar 7.0g/L+ sucrose 30g/L, pH=5.8,
Cultivation temperature be 27 DEG C, intensity of illumination be 2000~3000lx, day light application time be 11h/d under conditions of carry out subculture training
Support, subculture cycle is 25d, in the generation of subculture 4, obtains the substantial amounts of adventitious bud with stem disk；
D, stem tuber culture：The adventitious bud with stem disk that step C is obtained aseptically is cut into adventitious bud and two, stem disk
Point, the adventitious bud for cutting is returned and is seeded in the proliferated culture medium of step C, and squamous subculture is carried out again；And the stem disk for cutting goes
Except root, stem, Ye Hou, 2 ~ 4 pieces are divided into（Every piece of particle diameter is 0.3 ~ 0.5cm sizes）It is seeded in following stem tuber culture medium：
MS+6- benayl aminopurines (6-BA) 1.2mg/L+ methyl α-naphthyl acetates（NAA）0.3mg/L+ agar 7.0 g/L+ sucrose 60g/L, pH=5.8,
Cultivation temperature be 27 DEG C, intensity of illumination be 2000~3000lx, day light application time be 11h/d under conditions of carry out stem tuber culture
55d, treats that stem tuber is long and is planted to bottle outlet by diameter about 1.0cm.
E, transplanting：The stem tuber of D step bottle outlets is taken out, the culture medium on stem tuber is routinely cleaned, being put into mass concentration is
Sterilized in 0.1~0.2% carbendazim solution after 1~2min, it is laterite to transplant to mass ratio：The nutrient matrix of leaf mould=1 ︰ 1
In, 40% ~ 45% shade density is needed, routinely watered, fertilizing management, germination and emergence after 40 ~ 60d of growth, you can praised
Blue lily seedling.
Embodiment 3
A. the selection of explant and sterilizing：The stem apex and axillary bud chosen robust growth and do not form bud are explant, are peelled off outer
Layer blade, is cut into the segment of 1.5 ~ 2.0cm, is 0.1% in mass concentration after being cleaned with the conventional water added with appropriate detergent
Mercuric chloride solution in sterilize 10min, then the 3min that sterilized in the liquor natrii hypochloritis that mass concentration is 0.3%, then use sterilized water
Rinsing 3 times, then each 2min is blotted with aseptic filter paper, standby；
B, adventitious bud induction culture：Aseptically, following induction training will be inoculated in by the segment after step A sterilization
In foster base：MS+6- benayl aminopurines (6-BA) 8.0mg/L+ methyl α-naphthyl acetates（NAA）The g/L+ sucrose 30g/ of 0.05mg/L+ agar 6.8
L, pH=5.8, cultivation temperature be 27 DEG C, intensity of illumination be 2000~3000lx, light application time be 12h/d under conditions of, induction
Culture 20d differentiates 0.5 ~ 1.5cm adventitious buds until explant is sprouted；
C, adventitious bud shoot proliferation culture：The adventitious bud of step B is aseptically cut and is placed in following proliferated culture medium：
MS+6- benayl aminopurines (6-BA) 5.0mg/L+ methyl α-naphthyl acetates（NAA）0.1mg/L+ agar 6.8g/L+ sucrose 30g/L, pH=5.8,
Cultivation temperature be 27 DEG C, intensity of illumination be 2000~3000lx, day light application time be 12h/d under conditions of carry out subculture training
Support, subculture cycle is 20d, in the generation of subculture 5, obtains the substantial amounts of adventitious bud with stem disk；
D, stem tuber culture：The adventitious bud with stem disk that step C is obtained aseptically is cut into adventitious bud and two, stem disk
Point, the adventitious bud for cutting is returned and is seeded in the proliferated culture medium of step C, and squamous subculture is carried out again；And the stem disk for cutting goes
Except root, stem, Ye Hou, 2 ~ 4 pieces are divided into（Every piece of particle diameter is 0.3 ~ 0.5cm sizes）It is seeded in following stem tuber culture medium：
MS+6- benayl aminopurines (6-BA) 1.5mg/L+ methyl α-naphthyl acetates（NAA）0.5mg/L+ agar 6.8 g/L+ sucrose 60g/L, pH=5.8,
Cultivation temperature be 27 DEG C, intensity of illumination be 2000~3000lx, day light application time be 12h/d under conditions of carry out stem tuber culture
50d, treats that stem tuber is long and is planted to bottle outlet by diameter about 1.0cm.
E, transplanting：The stem tuber of D step bottle outlets is taken out, the culture medium on stem tuber is routinely cleaned, being put into mass concentration is
Sterilized in 0.1~0.2% carbendazim solution after 1~2min, it is laterite to transplant to mass ratio：The nutrient matrix of leaf mould=1 ︰ 1
In, 40% ~ 45% shade density is needed, routinely watered, fertilizing management, germination and emergence after 40 ~ 60d of growth, you can praised
Blue lily seedling.
Contrast test：
1. material to be tested：Good orchid lily cultivar ' large-fruited Chinese hawthorn '；
2. test method：With embodiment 1；
3. result of the test：
3.1 hormons are with the influence for comparing adventitious bud inducing
In for 8 Medium Proportions of examination, inductivity difference is big, and adventitious bud inducing effect is preferable when 6-BA concentration is high.From table 1
It can be seen that, treatment, it is 92.1% when 6-BA is inductivity highest when 8.0mg/L, NAA are 0.01mg/L；Next to that treatment, it is
85.0%。
The hormon of table 1 is with the influence for comparing adventitious bud inducing
3.2 hormons are with the influence for comparing adventitious bud proliferation
Growing state of the adventitious bud in hormon concentration cultures is shown in Table 2.6-BA excessive concentrations, adventitious bud growth is thin and delicate,
Vitrifying is serious；When 6-BA concentration is in 4.0 ~ 5.0mg/L, adventitious bud proliferation multiple is high, plant strain growth is normal.
The hormon of table 2 is with the influence for comparing adventitious bud proliferation
Influence of 3.3 different sucroses to tuber character
Sucrose concentration influences the formation of stem tuber.Sucrose concentration is low, and the formation rate of stem tuber is also low.As seen from Table 3, when sucrose concentration reaches
During 60g/L, the formation rate highest of stem tuber, up to 84%；
Influence of the different sucrose of table 3 to tuber character
General principle of the invention, principal character and advantages of the present invention has been shown and described above.The technical staff of the industry
It should be appreciated that the present invention is not limited to the above embodiments, the present invention is simply illustrated described in above-described embodiment and specification
Principle, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these change and
Improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appending claims and its equivalent
Thing is defined.
Claims (4)
1. a kind of method of good blue Rapid Propagation of Lilium, it is characterised in that comprise the following steps：
A, the selection of explant and sterilizing：The stem apex and/or axillary bud chosen robust growth and do not form bud are explant, are peelled off
Outer blade, is cut into the segment of a length of 1.5 ~ 2.0cm, cleans up and sterilizes, then extremely residual without disinfectant with rinsed with sterile water
Stay；
B, adventitious bud induction culture：To be inoculated in adventitious bud induction culture base by the segment after step A sterilization, in training
Support temperature be 27 ± 2 DEG C, intensity of illumination be 2000~3000lx, day light application time be 10~12h/d under conditions of, Fiber differentiation
20 ~ 40d differentiates 0.5 ~ 1.5cm adventitious buds long until explant is sprouted；
Described adventitious bud induction culture base is：MS culture mediums+6.0mg/L ~ 8.0mg/L 6-benzyl aminopurines+0.01mg/L ~
0.05mg/L methyl α-naphthyl acetates+6.5g/L ~ 7.0g/L agar+30g/L sucrose, pH5.8 ~ 6.0；
C, adventitious bud shoot proliferation culture：The adventitious bud of step B is aseptically cut and is placed in proliferated culture medium, in training
Support temperature be 27 ± 2 DEG C, intensity of illumination be 2000~3000lx, day light application time be 10~12h/d under conditions of carry out subculture
Culture, obtains the adventitious bud with stem disk；
Described proliferated culture medium MS culture mediums+4.0mg/L ~ 5.0mg/L 6-benzyl aminopurines+0.05mg/L ~ 0.1mg/L naphthalenes
Acetic acid+agar 6.5g/L ~ 7.0g/L+ sucrose 30g/L, pH5.8 ~ 6.0；
D, stem tuber culture：The adventitious bud with stem disk that step C is obtained aseptically is cut into adventitious bud and two, stem disk
Point, the adventitious bud for cutting is returned and is seeded in the proliferated culture medium of step C, and squamous subculture is carried out again；And the stem disk for cutting goes
Except root, stem, Ye Hou, it is divided into 2 ~ 4 pieces and is seeded in stem tuber culture medium, be 27 ± 2 DEG C in cultivation temperature, intensity of illumination is
2000~3000lx, day light application time be 10~12h/d under conditions of carry out stem tuber 50 ~ 60d of culture, treat that stem tuber is long to diameter
Planted under bottle outlet by 1.0 ~ 1.5cm；
Described stem tuber culture medium is：MS culture mediums+1.0mg/L ~ 1.5mg/L 6-benzyl aminopurines+0.1mg/L ~ 0.5mg/L
Methyl α-naphthyl acetate+6.5g/L ~ 7.0g/L agar+60g/L sucrose, pH5.8 ~ 6.0；
E, transplanting：The stem tuber of D step bottle outlets is routinely cleaned the culture medium on stem tuber, it is 0.1~0.2% to be put into mass concentration
Carbendazim solution in sterilize 1~2min after, transplant to mass ratio be laterite：In the nutrient matrix of leaf mould=1 ︰ 1, need 40% ~
45% shade density, is routinely watered, fertilizing management, germination and emergence after 40 ~ 60d of growth, you can obtain good orchid lily kind
Seedling.
2. the method for good blue Rapid Propagation of Lilium according to claim 1, it is characterised in that the cleaning described in step A
It is after being cleaned with the conventional water added with detergent, to disappear in the mercuric chloride solution that mass concentration is 0.1% with the specific method of sterilization
7 ~ 10min of poison, then the 3 ~ 5min that sterilized in the liquor natrii hypochloritis that mass concentration is 0.3%, then with rinsed with sterile water 3 ~ 4 times.
3. the method for good blue Rapid Propagation of Lilium according to claim 1, it is characterised in that described subculture cycle is 20
~ 30d, in the generation of subculture 3 ~ 5, can obtain a large amount of adventitious buds with stem disk.
4. the method for good blue Rapid Propagation of Lilium according to claim 1, it is characterised in that in step D, the stem disk for cutting
Removal root, stem, Ye Hou, are divided into 2 ~ 4 pieces, and every piece of particle diameter is 0.3 ~ 0.5cm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109430059A (en) * | 2018-12-25 | 2019-03-08 | 云南省农业科学院花卉研究所 | A kind of cut-flower Ornithogalum caudatum in vitro culture quick-breeding method |
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