CN106706430A - Testing device for active biologic biaxial drawing and application thereof in soft material - Google Patents
Testing device for active biologic biaxial drawing and application thereof in soft material Download PDFInfo
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- CN106706430A CN106706430A CN201710142138.9A CN201710142138A CN106706430A CN 106706430 A CN106706430 A CN 106706430A CN 201710142138 A CN201710142138 A CN 201710142138A CN 106706430 A CN106706430 A CN 106706430A
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- Prior art keywords
- tissue
- active bio
- biaxial stretch
- test device
- liquor box
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N3/00—Investigating strength properties of solid materials by application of mechanical stress
- G01N3/08—Investigating strength properties of solid materials by application of mechanical stress by applying steady tensile or compressive forces
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2203/00—Investigating strength properties of solid materials by application of mechanical stress
- G01N2203/0014—Type of force applied
- G01N2203/0016—Tensile or compressive
- G01N2203/0017—Tensile
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
- Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
Abstract
The invention discloses a testing device for active biological tissue biaxial drawing and application thereof in a soft material. The testing device comprises a solution tank, four baffle plates for reducing solution evaporation are arranged at four corners of the top of the solution tank; a PBS buffer solution for maintaining biological tissue activity is loaded in the solution tank; a cylindrical lens slot for storing a waterproof camera is formed in the bottom of the solution tank; a support frame is arranged above the baffle plates, a servo cylinder is fixed on each of four directions of the support frame; the front end of each servo cylinder is orderly connected with a force sensor and a connecting piece; four connecting pieces are respectively connected with the upper parts of four long levers vertically arranged in the solution tank; the middle parts of the four long levers are connected with a middle support plate playing a fulcrum effect; and the lower parts of the four long levers are connected with a clamp for fixing the biological tissue.
Description
Technical field
It is more particularly to a kind of biaxial stretch-formed for active bio tissue the present invention relates to biomechanical property testing field
Test device and the application in other soft material fields.
Background technology
In virtual operation physical modeling, accurate physical model to the reliability of surgical simulation it is critical that, structure
The basis that rationally and accurately constitutive equation is physical model is built, due to the anisotropy of biological tissue, biaxial tension experiment can
Determine constitutive equation to mechanical behavior and material property for measurement each of biological organization material.On the other hand, in biology
Organize in vitro Mechanics Performance Testing, the activity of tissues in vitro cannot ensure the problem in always test.
There is clearly disadvantageous and limitation in existing biaxial stretch-formed experimental provision, some specially measure material mechanical performance
Biaxial stretch-formed device cannot be applied to active bio tissue test, such as application publication number is CN105092371A,
The patents of invention such as CN102645371A, CN104913974A.Wherein application publication number is that the invention of CN105092371A is proposed
A kind of adjustable biaxial stretch-formed fixture of stretch ratio, including base, drawing plate and installing plate, install left and right and are oriented on the base of left and right
Post, regulation lead column can change biaxial stretch-formed ratio.The invention possesses stretching high precision, advantages of simple structure and simple, but
Application category is confined to composite etc., it is impossible to suitable for active bio organization material.
A kind of biaxial tension-compression strength device disclosed in the patent of invention of application publication number CN102645371A, including frame,
First screw mandrel, the second leading screw, the first drive mechanism and the second drive mechanism, employ both sides different on each group of draw direction
Same ball-screw of curl, can effectively realize the biaxial tension to test specimen different proportion, and improve test result
Precision.Although the structure design of foregoing invention is quite reasonable, invention cannot be applied to the test of active bio tissue.Application
The invention of publication number CN104913974A be related to material Micro Mechanical Properties it is biaxial stretch-formed-fatigue test system, by precision plus
The part such as load-gear unit, tired unit, mechanics and deformation signal detection unit, specimen holder unit constitutes, can be real respectively
Existing uniaxial tensile test, biaxial stretch-formed test, testing fatigue etc., and have good compatibility with light microscope, can carry out
The rules such as the heterogeneous microstructure and deformation damage mechanism of dynamic studies material.Although the invention is capable of achieving single biaxial stretch-formed and tired
Labor is tested, but invention cannot be applied to the test of active bio tissue.
The drawbacks of for each patent of invention above and deficiency, it is proposed that a kind of biaxial stretch-formed for active bio tissue
Test device.
Retrieval more than finds that above technical scheme can not influence novelty of the invention；And above patent text
Being mutually combined for part can not destroy creativeness of the invention.
The content of the invention
In vitro single, double axle tensile mechanical properties examination can be carried out to active bio tissue it is an object of the invention to provide one kind
The extension test platform tested.
In order to realize the above object the technical solution adopted by the present invention is as follows：
A kind of test device biaxial stretch-formed for active bio tissue, including liquor box, at the top of described liquor box
Corner is provided with four baffle plates for reducing solution evaporation, and liquor box inside is molten equipped with the PBS cushioning liquid for keeping biological tissue activity
Liquid case bottom is provided with the cylindrical lens groove for placing water-proof CCD camera；A support frame is provided with described baffle plate,
Each be fixed with a servoBcylinder on the four direction of described support frame, each servoBcylinder front end successively with force snesor, even
Fitting is connected, and four connectors are connected with four long lever tops being vertically provided inside liquor box respectively, four long levers
Middle part has connected the middle part support plate of point action, and four long lever bottoms are each connected to the fixture of fixed biological tissue.
Further, described liquor box is that a top by one piece of PMMA base plate and four pieces of PMMA side plates bondings is opened
Mouth casing, is bonded cylindrical lens groove, for laying water-proof CCD camera, to shoot biaxial stretch-formed bioactivity tissue on base plate
Deformation.Appropriate PBS cushioning liquid is added in liquor box, for keeping biological tissue activity.
Further, described baffle plate is formed by a PMMA top board and two orthogonal PMMA side plates bondings, its point
Four angles not with liquor box cooperate, and when four baffle plates are arranged on four angles of liquor box, the top board of four baffle plates exists
One cross gap is formed on the top of liquor box, and the effect of the baffle plate is the volatilization for reducing solution in liquor box, while anti-
Only solution is splashed on support frame.
Further, described support frame is a supporter being made up of three-back-shaped casing, placed inside control circuit, four
Side midline top has servoBcylinder to be bolted, expansion link front end and force snesor and the connector successively phase of servoBcylinder
Even, promote connector to stretch out same distance by controller control servoBcylinder, and measure the change of institute's stress in real time.In twin shaft experiment
When order about four servoBcylinders while stretching out equidistant；The symmetrical servoBcylinder of two of which is ordered about in uniaxial tests while stretching out phase
Deng length.
Further, described long lever is four levers punched by strip aluminium alloy plate, wherein top is hinged
Hole is bolted connector, and bottom hinge hole is bolted fixture, and middle part is got multiple equidistant hinge holes and led to
The middle part support plate described in bolt connection is crossed, is hinged by different hinge holes and middle part support plate and is adapted to different size of biology
The biaxial tension-compression strength of active material, as well as lever principle, can apply more accurate displacement.When servoBcylinder is promoted
When force snesor and connector stretch out, connector promotes long lever, with the activity that middle part support plate is clamped as fulcrum to bottom jig
Biological tissue is stretched.
Further, described middle part support plate be one by one piece of square aluminium alloy plate and four connectors at four side midpoints
The part that place is welded, its effect is treated as four fulcrums of long lever, and the active bio tissue to long lever bottom is carried out
Stretching.
Further, described fixture is four fixtures being made up of PLA material, and its structure is a U-shape structure, U-shaped knot
The openend of structure carries out the clamping of active bio tissue, and active bio tissue, U-shape structure are bolted in openend both sides
Non- openend fixed with long lever bottom by bolt, activity is raw during fixture keeps drawing process with long lever angle change
The balance of thing tissue.
Further, application of the foregoing biaxial stretch-formed test device in soft material stretching experiment.
The invention has the advantages that：
1st, device simple structure, is detachably easy to process and safeguard, can be conveniently accomplished test mission.
2nd, the present invention can effectively realize that single shaft, biaxial tension-compression strength task are carried out to biological active mass to be ensured simultaneously
The activity of biological tissue；Specifically, when twin shaft is tested, controller orders about four servoBcylinders while stretching out equidistant；In single shaft
During experiment, controller orders about the symmetrical servoBcylinder of two of which while stretching out equal length.
3rd, due to using lever principle, the hinge hole of diverse location on long lever is hinged by middle part support plate, can be applied
More accurate displacement, with enough loading accuracies.
4th, when liquor box is vacant, device can also carry out the single, double axle stretching experiment of other soft materials.
Brief description of the drawings
The accompanying drawing for constituting the part of the application is used for providing a further understanding of the present invention, schematic reality of the invention
Apply example and its illustrate, for explaining the present invention, not constitute inappropriate limitation of the present invention.
Fig. 1 is schematic structural view of the invention.
Fig. 2 is internal structure schematic diagram.
Fig. 3 is liquor box top view.
Fig. 4 is the structure chart of fixture.
Description of reference numerals：
1st, liquor box；2nd, baffle plate；3rd, support frame；4th, servoBcylinder；5th, force snesor；6th, connector；7th, long lever；8th, middle part
Support plate；9th, fixture；10th, camera lens groove.
Specific embodiment
In order that those skilled in the art more fully understand technical scheme, the present invention is entered below in conjunction with the accompanying drawings
Row is described in detail, and the description of this part is only exemplary and explanatory, does not there is any limitation to protection scope of the present invention.
It is noted that described further below is all exemplary, it is intended to provide further instruction to the application.Unless another
Indicate, all technologies used herein and scientific terminology are with usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singulative
Be also intended to include plural form, additionally, it should be understood that, when in this manual use term "comprising" and/or " bag
Include " when, it indicates existing characteristics, step, operation, device, component and/or combinations thereof.
Double week tensile test apparatus of the present invention include liquor box, and corner is provided with four at the top of described liquor box
The baffle plate of solution evaporation is reduced, equipped with the PBS cushioning liquid for keeping biological tissue activity, liquor box bottom is provided with for liquor box inside
Cylindrical lens groove for placing water-proof CCD camera；A support frame, described support frame are provided with described baffle plate
Four direction on be each fixed with a servoBcylinder, each servoBcylinder front end is connected with force snesor, connector successively, four
Connector is connected with four long lever tops being vertically provided inside liquor box respectively, and four long lever middle parts have connected fulcrum
The middle part support plate of effect, four long lever bottoms are each connected to fix the fixture of stretching thing.
Specifically, respectively as shown in Figure 1, Figure 2, Figure 3, Figure 4, it is of the invention as a example by stretching active bio tissue, to this hair
Bright disclosed biaxial stretch-formed test device, is specifically described：It include liquor box 1, baffle plate 2, support frame 3, servoBcylinder 4,
Force snesor 5, connector 6, long lever 7, middle part support plate 8, fixture 9, camera lens groove 10；
Overall structure is：The top corner of liquor box 1 is provided with four baffle plates 2 for reducing solution evaporation, the inside of liquor box 1 dress
There is the PBS cushioning liquid for keeping biological tissue activity, the bottom of liquor box 1 is provided with the cylindrical lens for placing water-proof CCD camera
Groove 10；It is support frame 3 in the top of liquor box 1, four servoBcylinders 4 is bolted on support frame 3, controls to watch by controller
Distance of the cylinder 4 according to the different mobile settings of experiment is taken, each front end of servoBcylinder 4 is connected with force snesor 5, connector 6 successively,
Four connectors 6 are each bolted with four tops of long lever 7 respectively, and four middle parts of long lever 7 are common by bolt
One middle part support plate 8 for having connected point action, four bottoms of long lever 7 are used for fixed biology for one each via bolt connection
The structure of 9 four fixtures of fixture of tissue is entirely different.
Wherein, liquor box 1 is an opening casing being bonded by one piece of PMMA base plate and four blocks of PMMA side plates, base plate
Upper bonding cylindrical lens groove 10, for laying water-proof CCD camera, to shoot the deformation feelings of biaxial stretch-formed bioactivity tissue
Condition.
Specifically, the center in the cross gap that position of the cylindrical lens groove 10 on base plate is just formed with baffle plate 2
Position is relative up and down, and camera camera lens just to biological active mass.
Appropriate PBS cushioning liquid is added in liquor box 1, for keeping biological tissue activity.
Further, baffle plate 2 from a PMMA top board and two PMMA side plates bonding can with liquor box with, two PMMA
Side plate is mutually perpendicular to, and PMMA top boards are arranged on the top of side plate；The vertical angle that three board is formed just is matched somebody with somebody with the angle of liquor box
Close, when liquor box coordinates four baffle plates 2, a cross gap is formd at the top of liquor box 1, be arranged such baffle plate 2
Effect be reduce liquor box in solution volatilization, while preventing solution to be splashed on support frame 3.
Further, support frame 3 is a supporter being made up of three-back-shaped casing, placed inside control circuit, support
Midline top has servoBcylinder 4 to be bolted on four faces (East, West, South, North face) in body top, the expansion link of servoBcylinder 4
Front end is sequentially connected with force snesor 5 and connector 6, promotes connector 6 to stretch out same distance by controller control servoBcylinder 4,
And the change of the institute of measurement in real time stress.
Four servoBcylinders 4 are ordered about when twin shaft is tested while stretching out equidistant；Two of which pair is ordered about in uniaxial tests
The servoBcylinder 4 of title stretches out equal length simultaneously.
Further, long lever 7 is four levers punched by strip aluminium alloy plate, wherein top hinge hole leads to
Bolt connection connector 6 is crossed, bottom hinge hole is bolted fixture 9, and middle part is got multiple equidistant hinge holes and passed through
Middle part support plate 8 described in bolt connection, is hinged by different hinge holes and middle part support plate 8 and adapts to different size of biology
The biaxial tension-compression strength of active mass, as well as lever principle, can apply more accurate displacement.When servoBcylinder 4 is pushed away
When dynamic pickup 5 and connector 6 stretch out, connector 6 promotes long lever 7, with middle part support plate 8 for fulcrum is pressed from both sides to bottom jig 9
The active bio tissue held is stretched.
Middle part support plate 8 is one and is welded in four side midpoints by one piece of square aluminium alloy plate and four connectors 6
Part, its effect is treated as four fulcrums of long lever 7, and the active bio tissue to the bottom of long lever 7 is stretched.
Fixture 9 is as shown in figure 4, be four particular jigs being made up of PLA material, its structure is a U-shape structure, U-shaped
The openend of structure carries out the clamping of active bio tissue, and active bio tissue, U-shaped knot are bolted in openend both sides
The non-openend of structure is fixed by bolt with the bottom of long lever 7, and fixture keeps being lived in drawing process with the angle change of long lever 7
The balance of property biological tissue.
By above description, it is known that the present invention achieves following effect：
1st, device simple structure, only by 10 parts, i.e., (liquor box 1, baffle plate 2, the servoBcylinder 4, power of support frame 3 are passed
Sensor 5, connector 6, long lever 7, middle part support plate 8, fixture 9, camera lens groove 10) it is removably attachable to together, be achieved that whole
Individual experiment, due to being dismountable connection between each part, therefore is easy to process and safeguards, can be conveniently accomplished experiment and appoint
Business.
2nd, the present invention can effectively realize that single shaft, biaxial tension-compression strength task are carried out to biological active mass to be ensured simultaneously
The activity of biological tissue；Specifically, when twin shaft is tested, controller orders about four servoBcylinders while stretching out equidistant；In single shaft
During experiment, controller orders about the symmetrical servoBcylinder of two of which while stretching out equal length.
3rd, the present invention is hinged the hinge hole of diverse location on long lever by middle part support plate due to using lever principle, can
To apply more accurate displacement, with enough loading accuracies.
4th, when liquor box is vacant, device can also carry out the single, double axle stretching experiment of other soft materials.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be in other specific forms realized.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires to be limited rather than described above, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each implementation method is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should
Specification an as entirety, the technical scheme in each embodiment can also be formed into those skilled in the art through appropriately combined
May be appreciated other embodiment.
Claims (10)
1. a kind of test device biaxial stretch-formed for active bio tissue, it is characterised in that including liquor box, described molten
Liquid case top corner is provided with four baffle plates for reducing solution evaporation, and liquor box inside is equipped with and keeps the PBS of biological tissue activity to delay
Solution is rushed, liquor box bottom is provided with the cylindrical lens groove for placing water-proof CCD camera；One is provided with described baffle plate
Individual support frame, each a servoBcylinder is fixed with the four direction of described support frame, each servoBcylinder front end successively with power
Sensor, connector are connected, and four connectors are connected with four long lever tops being vertically provided inside liquor box respectively, and four
Individual long lever middle part has connected the middle part support plate of point action, and four long lever bottoms are connected to the folder of fixed biological tissue
Tool.
2. it is as claimed in claim 1 to be used for the biaxial stretch-formed test device of active bio tissue, it is characterised in that described is molten
Liquid case is an open top casing being bonded by one piece of PMMA base plate and four blocks of PMMA side plates, and circle is bonded on the base plate
Cylindricality camera lens groove, for laying water-proof CCD camera, to shoot the deformation of biaxial stretch-formed bioactivity tissue.
3. it is as claimed in claim 1 to be used for the biaxial stretch-formed test device of active bio tissue, it is characterised in that described is molten
Appropriate PBS cushioning liquid is added in liquid case, for keeping biological tissue activity.
4. it is as claimed in claim 1 to be used for the biaxial stretch-formed test device of active bio tissue, it is characterised in that described gear
Plate is formed by a PMMA top board and two orthogonal PMMA side plates bondings, its respectively with four angle phase interworkings of liquor box
Close, when four baffle plates are arranged on four angles of liquor box, four top boards of baffle plate form a cross at the top of liquor box
Shape gap.
5. it is as claimed in claim 1 to be used for the biaxial stretch-formed test device of active bio tissue, it is characterised in that described branch
Support is a supporter being made up of three-back-shaped casing, placed inside control circuit, four, the top center line in face of supporter
Place is respectively and fixedly provided with a servoBcylinder, and expansion link front end and the force snesor and connector of the servoBcylinder are sequentially connected, by controlling
Device control servoBcylinder promotes connector to stretch out the distance of setting, and measures the change of institute's stress in real time.
6. it is as claimed in claim 5 to be used for the biaxial stretch-formed test device of active bio tissue, it is characterised in that in twin shaft examination
When testing, controller orders about four servoBcylinders while stretching out equidistant；In uniaxial tests, it is symmetrical that controller orders about two of which
ServoBcylinder stretches out equal length simultaneously.
7. it is as claimed in claim 1 to be used for the biaxial stretch-formed test device of active bio tissue, it is characterised in that described length
Lever is four levers punched by strip aluminium alloy plate, wherein top hinge hole is bolted connector, bottom
Portion's hinge hole is bolted fixture, and middle part is got multiple equidistant hinge holes and is bolted described middle part branch
Plate, is hinged by different hinge holes and middle part support plate and adapts to the biaxial stretch-formed examination of different size of bioactive materials
Test.
8. it is as claimed in claim 1 to be used for the biaxial stretch-formed test device of active bio tissue, it is characterised in that in described
Portion's support plate is a part being welded in four side midpoints by one piece of square aluminium alloy plate and four connectors.
9. it is as claimed in claim 1 to be used for the biaxial stretch-formed test device of active bio tissue, it is characterised in that described folder
It is four fixtures being made up of PLA material to have, and its structure is a U-shape structure, and the openend of U-shape structure carries out active bio group
The clamping knitted, active bio tissue is bolted in openend both sides, and the non-openend of U-shape structure passes through bolt and Chang Gang
Bar bottom is fixed, and fixture keeps the balance of active bio tissue in drawing process with long lever angle change.
10. the test device biaxial stretch-formed for active bio tissue as described in claim 1-9 is any is stretched in soft material
Application in experiment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710142138.9A CN106706430B (en) | 2017-03-10 | 2017-03-10 | Testing device for active biological biaxial stretching and application of testing device in soft material |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710142138.9A CN106706430B (en) | 2017-03-10 | 2017-03-10 | Testing device for active biological biaxial stretching and application of testing device in soft material |
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| CN106706430A true CN106706430A (en) | 2017-05-24 |
| CN106706430B CN106706430B (en) | 2023-06-16 |
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| WO2019095409A1 (en) * | 2017-11-15 | 2019-05-23 | 江苏铂影数据科技有限公司 | Integrated multifunctional test instrument for biological reaction and soft tissue mechanics |
| CN113049362A (en) * | 2021-03-18 | 2021-06-29 | 凯尔测控试验系统（天津）有限公司 | Miniature in-situ biaxial mechanical testing machine for confocal microscope |
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