CN106701578A - Ventilation stirring system for bioreactor - Google Patents
Ventilation stirring system for bioreactor Download PDFInfo
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- CN106701578A CN106701578A CN201710163571.0A CN201710163571A CN106701578A CN 106701578 A CN106701578 A CN 106701578A CN 201710163571 A CN201710163571 A CN 201710163571A CN 106701578 A CN106701578 A CN 106701578A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/08—Stirrer or mobile mixing elements with different stirrer shapes in one shaft or axis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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Abstract
The invention discloses a ventilation stirring system for a bioreactor in the field of medical equipment. The system is arranged in a tank of the bioreactor and comprises a guide cylinder, a multistage speed regulation stirrer and an upper fixing plate and a lower fixing plate horizontally arranged in the tank, wherein the guide cylinder vertically penetrates through the center of the upper fixing plate and the center of the lower fixing plate; the upper fixing plate and the lower fixing plate are both porous plates so that a cell culture bed is formed among the tank, the guide cylinder, the upper fixing plate and the lower fixing plate; a vertical ventilation pipe is fixed on the inner peripheral wall of the guide cylinder; the top end of the ventilation pipe is higher than the top end of the guide cylinder; the bottom end of the ventilation pipe is communicated with an annular gas distributor which is of the same height as the lower fixing plate and the outer periphery of the ventilation pipe clings to the inner periphery of the guide cylinder; multiple oxygen supply ventilation holes are uniformly formed in the periphery of the annular gas distributor; and the multistage speed regulation stirrer vertically stretches to the bottom of the guide cylinder.
Description
Technical field
The present invention relates to a kind of air agitation system for bioreactor of medical device field.
Background technology
The cell culture bed of cell culture is carried out in current bioreactor, fluid bed and fixed bed is broadly divided into.It is fixed
Bed is that fixed container is assembled in tank body at one, and the filler that is made up of cell culture vector is filled with the middle of the container to enter
Row cell culture, the cell culture vector is typically diameter 2-6mm particulate vectors, porous ball or sheet-like fiber.Cell is trained
Active force flowing in cell culture bed that nutrient solution is produced by agitator in stirring, to carry out the biography of nutritional ingredient and oxygen
Pass.The cell lift agitator that NBS companies of U.S. mid-1980s in last century is released, by the rotation of agitator, produces centrifugation
Power, throws away nutrient solution from the centrifuge tube on top, then down through carrier layer, enters back into the bottom guide shell of lift agitator
Inside rise to top to throw away again, reach purpose of the nutrient solution in tank internal circulation flow.This lift agitator is by rotating generation
Centrifugal force, cell culture fluid forms negative pressure after top is thrown out of, and bottom cell culture fluid rises again.The circulation side of this agitator
Formula is that cell culture fluid rises from middle guide shell, then from top out down through carrier layer.But carrier is in deadweight
Under the influence of, inherently can deposition together, along with the active force that cell culture fluid is downward, can carrier layer be pressed tighter and tighter
Cell culture solution flow is caused to be obstructed, such tank inner cell nutrient solution circulation will heterogeneity, the uneven stream of cell culture fluid
Move and cause channeling and nutrient distribution uneven, cause vitro growth rates uniformity poor.
Especially after the amplification of reactor tank body, carrier bed is higher, and carrier overstocks more serious, and cell culture fluid penetration power is got over
Difference, tank inner cell nutrient solution circulation is poorer.So this agitator applies the presence in fixed bed culture cannot to amplify culture
Drawback.10L is only rested on by the equipment that client approves in this cell reactor that also exactly NBS companies in the U.S. introduce to the market at present
Glass jar.Client's have to take mode of tank battery culture is caused to meet Production requirement.
The content of the invention
The invention aims to overcome the deficiencies in the prior art, there is provided a kind of bioreactor, it contributes to right
Before cell culture bed carries out deep layer oxygen supply, the separation of dissolved oxygen and oxygen bubble is realized, prevent oxygen bubble from entering cell culture
Bed, bubble is damaged so as to prevent oxygen bubble from rupturing.
Realizing a kind of technical scheme of above-mentioned purpose is：A kind of one air agitation system for bioreactor, is placed in
In the tank body of the bioreactor, including guide shell, multistep speed regulation agitator, and be horizontally placed at upper solid in the tank body
Fixed board and bottom plate, the guide shell are described vertically through the center of fixed plate and the center of the bottom plate
Upper mounted plate and the bottom plate are porous plate, make the tank body, the guide shell, fixed plate and described lower solid
Cell culture bed is formed between fixed board；
The breather pipe being vertically arranged is fixed with the inner peripheral wall of the guide shell, the top of the breather pipe is higher than described
The top of guide shell, the bottom connection of the breather pipe is contour with the bottom plate, and excircle is close to the guide shell inner circle
The annular gas distributor in week, uniform multiple oxygen supply passage, the multistep speed regulation on the circumference of the annular gas distributor
Agitator stretches into the bottom of the guide shell vertically.
Further, the guide shell be divided into from top to bottom successively with one heart connection vertical draft tube, internal diameter it is up-small and down-big
Bell-mouth structure of air, and vertical stirring room, the vertical draft tube is through fixed plate and solid with fixed plate
Fixed, the top of the bell-mouth structure of air connects the bottom of the vertical draft tube, the bottom of the bell-mouth structure of air and institute with one heart
Bottom plate is stated to fix, the top of the vertical stirring room connect with one heart the bottom of the bell-mouth structure of air and with the lower fixation
Plate is fixed；The annular gas distributor is contour with the bottom plate and is close to the inner peripheral wall of the bell-mouth structure of air, institute
State multistep speed regulation agitator and stretch into vertical stirring room from the bottom of the tank body, the blowdown pipe be close to the vertical draft tube and
The inner peripheral wall of the bell-mouth structure of air.
Further, the inner peripheral wall of the vertical draft tube is fixed with the feed supplement pipe being vertically arranged, the feed supplement pipe
Top higher than the guide shell top and be less than the top of the breather pipe, bottom and bottom of the vertical draft tube etc.
It is high.
Further, the top of the feed supplement pipe is higher than the top 2cm of the vertical draft tube, the top of the breather pipe
End 3cm higher than the top of the vertical draft tube.
Further, the height that the multistep speed regulation agitator stretches into vertical stirring room part is the multistage tune
The a quarter of fast agitator total height is to 1/3rd.
Further, fixed by O-ring between fixed plate and the guide shell, the bottom plate with it is described
It is welded and fixed between guide shell, the excircle of fixed plate from inside to outside covers acting flange and packing ring, the lower fixation successively
The excircle of the excircle of plate from inside to outside covers acting flange and packing ring successively.
Further, the blade of the multistep speed regulation agitator is Ping Ye or propeller shape blade.
Further, the top of the blade of the multistep speed regulation agitator is less than the annular gas distributor.
Further, the quantity of the oxygen supply passage on the annular gas distributor is 36.
A kind of technical scheme of air agitation system for bioreactor of the invention is employed, the biology is placed in
In the tank body of reactor, including guide shell, multistep speed regulation agitator, and be horizontally placed at the upper mounted plate in the tank body and under
Fixed plate, the guide shell is vertically through the center of fixed plate and the center of the bottom plate, fixed plate
Porous plate is with the bottom plate, makes the tank body, between the guide shell, fixed plate and the bottom plate
Form cell culture bed；The breather pipe being vertically arranged, the top of the breather pipe are fixed with the inner peripheral wall of the guide shell
Higher than the top of the guide shell, the bottom connection of the breather pipe is contour with the bottom plate, and excircle is close to described leading
The annular gas distributor of flow cartridge inner periphery, uniform multiple oxygen supply passage, described on the circumference of the annular gas distributor
Multistep speed regulation agitator stretches into the bottom of the guide shell vertically.It has the technical effect that：It helps to enter to cell culture bed
Before row deep layer oxygen supply, the separation of dissolved oxygen and oxygen bubble is realized, prevent oxygen bubble from entering cell culture bed, so that anti-block
Gas bubbles burst and damage bubble.
Brief description of the drawings
Fig. 1 is a kind of structural representation of air agitation system for bioreactor of the invention.
Fig. 2 is a kind of air agitation system Center Vent tube and annular gas distributor for bioreactor of the invention
Structural representation.
Fig. 3 is the vertical view of annular gas distributor in a kind of air agitation system for bioreactor of the invention
Figure.
Specific embodiment
Fig. 1 is referred to, the present inventor is led to below in order to be able to preferably understand technical scheme
Specifically embodiment is crossed, and is described in detail with reference to accompanying drawing：
Refer to Fig. 1, a kind of air agitation system for bioreactor of the invention, positioned at the tank of bioreactor
In vivo, the bioreactor is fixed-bed bioreactor, including guide shell 18, feed supplement pipe 11, breather pipe 9, annular gas point
Cloth device 10, multistep speed regulation agitator 8, upper mounted plate 15 and bottom plate 17.
Wherein, bubble output hole and condenser mouthful (figure is not marked), the bubble output hole and the condensation are set at the top of the tank body
Device mouth is connected with condenser (figure is not marked), and the cell culture fluid in bioreactor enters the condensation from the bubble output hole
Device, and gas-liquid separation is carried out by the cyclone hydraulic separators in the condenser, the cell culture fluid by gas-liquid separation passes through institute
State condenser mouthful to return in the tank body, gas is discharged by the tail gas filter core and proportioning valve of the condenser.
The circumferential wall of the tank body is provided with four fluid infusions, and liquid outlet and electrode ports.The fluid infusion be used for
The alkali lye of addition cell culture fluid and regulation pH value in the tank body.
Upper mounted plate 15 and bottom plate 17 are horizontally placed in the tank body and are arranged concentrically.Upper mounted plate 15 it is cylindrical
The packing ring for flange being socketed with week and being socketed on flange excircle week, for realize the excircle of upper mounted plate 15 with it is described
Sealing between the inner peripheral wall of tank body.Flange is socketed with the excircle of bottom plate 17 and the flange excircle week is socketed in
On packing ring, for realizing the sealing between bottom plate 17 and the inner peripheral wall of the tank body.The Upper vertical of guide shell 18
Extend upward through the center of upper mounted plate 15, the center of the lower vertical of guide shell 18 downward through bottom plate 17.It is such to set
Meter makes the excircle of upper mounted plate 15 and the excircle of bottom plate 17 be separated with the inner peripheral wall of the tank body, therefore facilitates
The dismounting and installation of upper mounted plate 15 and bottom plate 17.
Guide shell 18 is divided into the up-small and down-big horn mouth knot of the vertical draft tube 12, internal diameter for connecting with one heart successively from top to bottom
Structure 13 and vertical stirring room 14.Vertical draft tube 12 runs through upper mounted plate 15 vertically.The top of vertical draft tube 12 is higher than upper solid
The top surface of fixed board 15, bottom is connected with the top of bell-mouth structure of air 13.Pass through O shapes between vertical draft tube 12 and upper mounted plate 15
Circle is fixed, and O-ring is connected in the draw-in groove of the inner periphery of upper mounted plate 15.That is, the inner periphery of upper mounted plate 15 with
Guide shell 15 is to separate, therefore is easy to the installation and removal of upper mounted plate 15.
Because a kind of air agitation system for bioreactor of the invention is easily installed or is separated with the tank body,
Therefore bioreactor can be made repeatedly to use, this greatly reduces cost for research work.
The bottom of bell-mouth structure of air 13 is welded and fixed with the top of bottom plate 17 and vertical stirring room 14, vertical stirring
The top of room 14 is welded and fixed with the bottom of bell-mouth structure of air 13 and bottom plate 17, so that bottom plate 17 and horn mouth
The position of structure 13 and the connection of vertical stirring room 14 is welded and fixed.The excircle of bell-mouth structure of air 13 is a conical surface.
The middle part of guide shell 18 sets the up-small and down-big bell-mouth structure of air 13 of internal diameter, beneficial to cell culture fluid in biological respinse
Circulation in device.Between the bottom surface of bottom plate 17 and the tank body formed back cavity 7, bottom plate 17, upper mounted plate 15 with
And cell culture bed is formed between guide shell 18 and the tank body, the bottom surface of upper mounted plate 15 is cell culture bed top surface, lower solid
The top surface of fixed board 17 is the bottom surface of cell culture bed.Multistep speed regulation agitator 8 stretches into vertical stirring room from the bottom of the tank body
14, the i.e. bottom of guide shell 18.Multistep speed regulation agitator 8 stretches into the height h2 of the part of vertical stirring room 14 for multistep speed regulation is stirred
The a quarter of the total height h1 of device 8 to 1/3rd, and the blade of multistep speed regulation agitator 8 top less than annular gas point
Cloth device 10.
Filled in cell culture bed between the bottom surface of upper mounted plate 15 and the top surface of bottom plate 17 and carried by cell culture
The filler 5 of body composition, cell culture vector generally uses sheet-like fiber carrier.It is upper mounted plate 15, lower solid in the present embodiment
Fixed board 17 is all porous plate, is made from porous stainless steel plate.The hole of the porous plate that upper mounted plate 15, bottom plate 17 are used
Footpath, it is necessary to less than the diameter of cell culture vector in filler 5, play the iris action to cell culture vector in filler 5, also prevent
The cell cultivated in cell culture vector is only caused to be subject to multistep speed regulation agitator 8 in filler 5 enters vertical stirring room 14
The effect of shearing force.The height d of filler 5 is 4th/2nd to five/3rds of cell culture bed height h.
The bottom of the tank body is provided with outlet valve 6 and sampling valve 4, and sampling valve 4 includes a valve rod, and the valve rod is from lower solid
The bottom insertion cell culture bed of fixed board 17, is sampled to the cell in cell culture bed.
In the tank body, cell culture fluid flows to the bottom of the tank body from guide shell 18, formed in guide shell 18 to
The swirling flow of lower flowing, the swirling flow that this flows downward is gone out into the tank from the bottom of the vertical stirring room 14 of the bottom of guide shell 18
Cell culture fluid is got rid of and is stirred to multistep speed regulation by the back cavity 7 of body bottom, the centrifugal force that multistep speed regulation agitator 8 is formed when rotating
Mix the lower section of device 8, cell culture fluid rebounded after the tank body bottom wall is encountered upwards in back cavity 7 dispersion then form to
The swirling flow of upper flowing.Because upper mounted plate 15 and bottom plate 17 are porous plate, the swirling flow that this is flowed up is from bottom plate
17 bottom enters filler 5, is cells with nutrient and dissolved oxygen in filler 5 in cell culture vector.What this was flowed up
Swirling flow continues up flowing, and through upper mounted plate 15, simultaneously as the effect of the centrifugal force of multistep speed regulation agitator 8, multistage is adjusted
At fast agitator 8 formed negative pressure, the tank body inner cell nutrient solution just from top through the internal reflux of guide shell 18 to back cavity 7,
Rebounded after the rotation of multistep speed regulation agitator 8 is downward again and risen, so as to reach the purpose of tank interior circulation.So cell culture
Cell culture vector in bed is settled downwards, and cell culture bed inner cell nutrient solution is flowed up in speed and cell culture bed
In the case that the speed of cell culture vector sedimentation tends to balance, the suspension of cell culture vector is realized.Other the one of back cavity 7
Individual purpose is to reduce the circulation dead angle in cell culture fluid cyclic process.
In the present embodiment, by the mixing speed for changing multistep speed regulation agitator 8, thus it is possible to vary the swirling flow for flowing up
The upward movement velocity in the filler 5.When multistep speed regulation agitator 8 is in zero span, in the back cavity 7 of the tank base
The swirling flow for flowing up cannot be formed.When the rotary speed of multistep speed regulation agitator 8 is in a grade of lowest speed, this is to upstream
The speed that dynamic swirling flow is flowed up is gone out a little less than cell culture vector in filler 5, and cell culture vector can be with filler 5
Settled down in cell culture bed, form the training method of similar fixed bed culture.When the rotation speed of multistep speed regulation agitator 8
When degree is in a grade of middling speed, there is larger gap in filler 5 between cell culture vector, and be observed that the cell
Culture carrier carry out it is small rock, this rocking helps to make cell culture fluid being uniformly distributed in filler 5, than existing
Fixed bed training method it is superior.
When the rotary speed of multistep speed regulation agitator 8 continues to rise, i.e., the rotary speed of multistep speed regulation agitator 8 is in most
At N grades of high speed, sedimentation speed of the speed that the swirling flow for flowing up is flowed up higher than cell culture vector in filler 5
Degree, make cell culture vector in filler 5 in suspended state, form the training method similar to fluid bed.Due to upper fixation
The porous plate that plate 15 and bottom plate 17 are used, its aperture is respectively less than the diameter of cell culture vector in filler 5, in filler 5
Cell culture vector is intercepted, without going out.Such design avoids the uneven flowing of cell culture fluid and makes
Uneven into channeling and nutrient distribution, the nutrition needed for ensure that cell growth is evenly distributed in cell culture bed,
Cell growth uniformity is good.Thus overcome using solidification bed carry out cell culture when because carrier overstock, cell culture fluid stream
It is dynamic to be obstructed, cause flowing that cell culture fluid is uneven and cause channeling and nutrient distribution uneven, so as to cause cell
The inconsistent defect of the speed of growth.Culture can be amplified to the cell in cell culture bed.
Vertical breather pipe 9, the top of the top than guide shell 18 of breather pipe 9 are fixed with the inner peripheral wall of guide shell 18
3cm high, the bottom of breather pipe 9 extends to the bottom of bell-mouth structure of air 13 along the inner peripheral wall of guide shell 18.The bottom of breather pipe 9
Portion's connection is set around the bottom inner peripheral wall of bell-mouth structure of air 13, i.e., the diameter of excircle is equal to the bottom inner circle of bell-mouth structure of air 13
The diameter in week, and the annular gas distributor 10 contour with bottom plate 17, annular gas distributor 10 are one and are horizontally disposed with
Ring pipe, breather pipe 9 is seamlessly connected and connects with annular gas distributor 10, uniform on the circumference of annular gas distributor 10
There are 36 oxygen supply passages 101.The oxygen of external world's input is transported to annular gas distributor 10, and lead to by breather pipe 9
Cross on annular gas distributor 10 36 keeping passages 101, under the active force of multistep speed regulation agitator 8 fully with
Cell culture fluid mixes, and most of bubble is crushed by multistep speed regulation agitator 8, and oxygen is completely dissolved in cell culture fluid, shape
Into the dissolved oxygen for being dissolved in cell culture fluid, and filler 5 is entered by the bottom of bottom plate 17, to cell culture in filler 5
Carrying the cell of In vivo culture carries out deep layer oxygen supply, and the bubble containing oxygen of some unbroken then drives in the wrong direction upwards through guide shell 18
To tank body top, discharged through the bubble output hole on the tank body, be not directly contacted with cell to reduce opening for the bubbles burst
Damage of the power to the cell culture vector inner cell.That is, annular gas distributor 10 contributes to extension oxygen entering
Enter the stroke before cell culture fluid, before deep layer oxygen supply is carried out to cell culture bed, realize the separation of dissolved oxygen and oxygen bubble,
Prevent oxygen bubble from entering cell culture bed, so as to prevent tension force when oxygen bubble ruptures and damaging cells.
Meanwhile, the inner peripheral wall of mozzle 12 is fixed with four feed supplement pipes 11 being vertically arranged, and the top of feed supplement pipe 11 is high
In the top of guide shell 18, the i.e. top of vertical draft tube 12, and less than the top of breather pipe 9.The bottom of feed supplement pipe 11 with it is vertical
The bottom of mozzle 12 is contour.The alkali lye of cell culture fluid and regulation pH value is introduced into guide shell 18 and exists by feed supplement pipe 11
Be sufficiently mixed in the presence of multistep speed regulation agitator 8 it is uniform after enter back into cell culture bed, it is to avoid the cell culture added
Liquid and alkali lye are directly entered cell culture bed, cause that the moment of cell culture environment drastically changes and cell growth is caused not
Profit influence.In the present embodiment, the top of the top than guide shell 18 of feed supplement pipe 11, the i.e. top 2cm high of vertical draft tube 12 leads to
The top of tracheae 9 than guide shell 18 top, i.e. the top 3cm high of vertical draft tube 12, with ensure to cell culture bed depth
Layer oxygen supply and deep layer are supported, and prevent the generation of bubble during deep layer oxygen supply and deep layer keeping.
In order to ensure the speed governing of multistep speed regulation agitator 8, the blade that multistep speed regulation agitator 8 is used is Ping Ye or propeller
Shape blade.
The bioreactor culture mode, the rotary speed of multistep speed regulation agitator 8 based on middling speed, interval and briefly
Change the rotary speed of multistep speed regulation agitator 8, make cell culture vector in flour filler 5 in suspended state, form similar
In the training method of fluid bed, cell culture fluid and metabolite in bioreactor is set to homogenize.The bioreactor is excellent
The culture of choosing may be set to：The training method culture of fixed bed 1-3 hours, the training method culture of fluid bed 1-3 minutes stops
Stirring 0.5-1 minutes, then restart to carry out cell culture using the training method of fixed bed.
It is highdensity thin that a kind of air agitation system for bioreactor of the invention can realize bioreactor
Born of the same parents cultivate, and the cell culture density in the cell culture bed is up to 3-5 × 107Individual/more than ml, and can carry out connecting for a long time
Continuous culture, this is to supply cell culture fluid and dissolved oxygen by the continuous bottom toward the tank body, while the top of the tank body
Supernatant is exported to realize.In filler 5, cell has good population effect in cell culture vector growth inside, is difficult to take off
Fall.Therefore the amplification potential of bioreactor is ensure that, highdensity cell culture can be realized in cell culture bed.The life
Thing reactor is particularly suitable for free serum culture, for a long time continuous perfusion, long-time harvesting metabolite.
Those of ordinary skill in the art it should be appreciated that the embodiment of the above be intended merely to explanation the present invention,
And be not used as being limitation of the invention, as long as in spirit of the invention, the change to embodiment described above
Change, modification will all fall in the range of claims of the present invention.
Claims (9)
1. a kind of air agitation system for bioreactor, is placed in the tank body of the bioreactor, including guide shell,
Multistep speed regulation agitator, and upper mounted plate and bottom plate in the tank body are horizontally placed at, the guide shell runs through vertically
The center of fixed plate and the center of the bottom plate, fixed plate and the bottom plate are porous plate,
Make the tank body, cell culture bed is formed between the guide shell, fixed plate and the bottom plate；Its feature exists
In：
The breather pipe being vertically arranged is fixed with the inner peripheral wall of the guide shell, the top of the breather pipe is higher than the water conservancy diversion
The top of cylinder, the bottom connection of the breather pipe is contour with the bottom plate, and excircle is close to the guide shell inner periphery
Annular gas distributor, uniform multiple oxygen supply passage on the circumference of the annular gas distributor, the multistep speed regulation stirring
Device stretches into the bottom of the guide shell vertically.
2. a kind of air agitation system for bioreactor according to claim 1, it is characterised in that：The water conservancy diversion
Cylinder is divided into from top to bottom the up-small and down-big bell-mouth structure of air of the vertical draft tube of concentric connection, internal diameter successively, and vertical stirring
Room, the vertical draft tube is fixed through fixed plate and with fixed plate, and the top of the bell-mouth structure of air is same
The heart connects the bottom of the vertical draft tube, and the bottom of the bell-mouth structure of air is fixed with the bottom plate, described vertically to stir
The top for mixing room connects the bottom of the bell-mouth structure of air and is fixed with the bottom plate with one heart；The annular gas distributor
Inner peripheral wall that is contour with the bottom plate and being close to the bell-mouth structure of air, the multistep speed regulation agitator is from the tank body
Bottom stretch into vertical stirring room, the blowdown pipe is close to the inner peripheral wall of the vertical draft tube and the bell-mouth structure of air.
3. a kind of air agitation system for bioreactor according to claim 2, it is characterised in that：It is described vertical
The inner peripheral wall of mozzle is fixed with the feed supplement pipe being vertically arranged, the top of the feed supplement pipe higher than the guide shell top simultaneously
Less than the top of the breather pipe, bottom is contour with the bottom of the vertical draft tube.
4. a kind of air agitation system for bioreactor according to claim 2, it is characterised in that：The feed supplement
The top of pipe is higher than the top 2cm of the vertical draft tube, and the top of the breather pipe is higher than the top of the vertical draft tube
3cm。
5. a kind of air agitation system for bioreactor according to claim 2, it is characterised in that：The multistage
The height that speed-adjustable stir device stretches into vertical stirring room part is that a quarter of the multistep speed regulation agitator total height is arrived
1/3rd.
6. a kind of air agitation system for bioreactor according to claim 2, it is characterised in that：It is described solid
Fixed by O-ring between fixed board and the guide shell, be welded and fixed between the bottom plate and the guide shell, it is described on
The excircle of fixed plate from inside to outside covers acting flange and packing ring successively, and the excircle of the excircle of the bottom plate is from inside to outside
Acting flange and packing ring are covered successively.
7. a kind of air agitation system for bioreactor according to claim 1, it is characterised in that：The multistage
The blade of speed-adjustable stir device is Ping Ye or propeller shape blade.
8. a kind of air agitation system for bioreactor according to claim 1, it is characterised in that：The multistage
The top of the blade of speed-adjustable stir device is less than the annular gas distributor.
9. a kind of air agitation system for bioreactor according to claim 1, it is characterised in that：The annular
The quantity of the oxygen supply passage on gas distributor is 36.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710163571.0A CN106701578A (en) | 2017-03-15 | 2017-03-15 | Ventilation stirring system for bioreactor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710163571.0A CN106701578A (en) | 2017-03-15 | 2017-03-15 | Ventilation stirring system for bioreactor |
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| CN106701578A true CN106701578A (en) | 2017-05-24 |
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| CN201710163571.0A Pending CN106701578A (en) | 2017-03-15 | 2017-03-15 | Ventilation stirring system for bioreactor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108315226A (en) * | 2018-03-15 | 2018-07-24 | 郭敬承 | A kind of center air lift type high-efficient biological reactor |
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| CN101768540A (en) * | 2010-02-12 | 2010-07-07 | 中国科学院广州能源研究所 | Reaction device for producing organic acid and alcohol through synthesis gas fermentation |
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Application publication date: 20170524 |