CN106691937A - Forsythia flower extract for inhibiting tyrosinase, and preparation method thereof and application - Google Patents
Forsythia flower extract for inhibiting tyrosinase, and preparation method thereof and application Download PDFInfo
- Publication number
- CN106691937A CN106691937A CN201710003219.0A CN201710003219A CN106691937A CN 106691937 A CN106691937 A CN 106691937A CN 201710003219 A CN201710003219 A CN 201710003219A CN 106691937 A CN106691937 A CN 106691937A
- Authority
- CN
- China
- Prior art keywords
- capsule
- flower extract
- weeping forsythia
- tyrosinase
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Cosmetics (AREA)
Abstract
The invention provides a forsythia flower extract for inhibiting tyrosinase, and a preparation method thereof and application. The forsythia flower extract is prepared by making forsythia flower undergo extraction by a hydrophilic solvent and preparing and separating effective ingredients through column chromatography. In the extract, the content of total flavonoids is over 12.0%, and the IC50 value of inhibiting tyrosinase activity is 0.67 mg/mL and is close to 0.52 mg/mL of the IC50 value of arbutin inhibiting tyrosinase. Since tyrosinase is a key enzyme for synthesizing melanin, the extract has good tyrosinase inhibiting activity, the extract has a potential beauty effect against chloasma and malar rash and the like caused by melanin pigmentation abnormity and can be applied to cosmetic development.
Description
Technical field
The present invention relates to cosmetic technical field, specifically a kind of capsule of weeping forsythia flower extract for suppressing tyrosinase and its
Preparation method and application.
Background technology
Chloasma, the butterfly macules of human face belong to melanin pigmentation and cause extremely, and tyrosinase is synthesis of melanin
Key enzyme, by suppressing the bioactivity of tyrosinase, can indirectly suppress the generation of melanin.Therefore, current nti-freckle,
Main component is with the kojic acid with suppression tyrosinase activity, based on ursin in skin-lightening cosmetic.And from natural plants
Middle screening tyrosinase inhibitor is simultaneously applied to the focus that always cosmetics are studied in nti-freckle, skin-lightening cosmetic.Such as aloe, sweet
The extract of grass and mulberry tree is used widely in nti-freckle, skin-lightening cosmetic.
Flos Forsythiae is the flower of the Oleaceae forsythia capsule of weeping forsythia, containing the biological flavone composition such as abundant rutin, and is had
Stronger anti-oxidant and antibacterial activity.And among the people with the custom drunk as jasmine tea using Flos Forsythiae.
The content of the invention
It is an object of the invention to provide a kind of capsule of weeping forsythia flower extract for suppressing tyrosinase and preparation method thereof and
Using.
A kind of preparation method for suppressing the capsule of weeping forsythia flower extract of tyrosinase that the present invention is provided, including following step
Suddenly：
(1) extract：Weigh a certain amount of capsule of weeping forsythia dried flower, crush, add 8~10 times of hydrophilic solvents of amount, be standing and soak for
30min, 1~2h of refluxing extraction, filtering, residue is again with 8~10 times of hydrophilic solvent refluxing extraction 1h of amount, filtering, merging two
Secondary extract solution, 60 DEG C are concentrated under reduced pressure into the Flos Forsythiae concentrate that proportion is 1.05~1.10；
(2) separate：Concentrate is washed with deionized water de- 2 first further with non-polar column chromatographic material chromatography
~4 column volumes, then with 20%, 40%, 60% ethanol water successively respectively elute 2~4 column volumes, collect 60% second
The eluent of alcohol solution, is concentrated under reduced pressure, dries, and obtains capsule of weeping forsythia flower extract.
Hydrophilic solvent described in step (1) can be that the mixing of the water and ethanol of water, ethanol, methyl alcohol or different proportion is molten
Agent, the water of different proportion and the mixed solvent of methyl alcohol, preferably use 70% ethanol water.
Non-polar column chromatographic material described in step (2) can be C18With MDS-5 any one.
Effective constituent determination：Using NaNO2-A1(NO3)3General flavone content in-NaOH colorimetric method for determining active components,
The content of general flavone (general flavone content is calculated with rutin equivalent) more than 12.0% in the active component of Flos Forsythiae.
Capsule of weeping forsythia flower extract with above-mentioned acquisition, can be with carrier, other beauty-care activity components, diluent as active component
Etc. being mixed and made into the specific functionality cosmetics such as toner, emulsion, condensation, facial mask and face cream.
The invention has the advantages that：Flos Forsythiae extractive total flavone content prepared by the present invention is (total for more than 12.0%
Flavones content is calculated with rutin equivalent).Capsule of weeping forsythia flower extract suppresses the IC of tyrosinase activity50It is 0.67mg/mL to be worth, and suppresses effect
Fruit is suitable with ursin, and (ursin suppresses the IC of tyrosinase50Value 0.52mg/mL).It can be answered in nti-freckle, skin-lightening cosmetic
With.
Specific embodiment
With reference to specific embodiment, the present invention is furture elucidated.
Embodiment 1 weighs 100g capsules of weeping forsythia dried flower, the appropriate ethanol water crushed, add 1000mL 70%, stands leaching
Bubble 30min, refluxing extraction 1h, filtering, residue is again with the ethanol water refluxing extraction 1h of 1000mL 70%, filtering, merging two
Secondary filtrate, it is 1.05 to be concentrated under reduced pressure into concentrate proportion.Concentrate is further with nonpolar C18Column chromatography material chromatography,
First it is washed with deionized water de- 3 column volumes, then 3 column volumes is respectively eluted successively with 20%, 40%, 60% ethanol water,
Deionized water and 20%, 40% ethanol water wash-out liquid are discarded, the eluent of 60% ethanol water is collected, depressurized dense
Contract, dry, obtain capsule of weeping forsythia flower extract.In the capsule of weeping forsythia flower extract content of general flavone be 12.5% (general flavone content with rutin work as
Amount is calculated).
Embodiment 2 weighs 100g capsules of weeping forsythia dried flower, the appropriate ethanol water crushed, add 800mL 70%, is standing and soak for
30min, refluxing extraction 1.5h, filtering, residue is again with the ethanol water refluxing extraction 1h of 800mL 70%, filtering, merging two
Secondary filtrate, it is 1.10, recycling design to be concentrated under reduced pressure into concentrate proportion.Concentrate is further with nonpolar MDS-5 column chromatographies
Material chromatography, is first washed with deionized water de- 4 column volumes, then is eluted successively with 20%, 40%, 60% ethanol water
4 column volumes, discard deionized water and 20%, 40% ethanol water wash-out liquid, and the ethanol water for collecting 60% is washed
It is de-, it is concentrated under reduced pressure, dries, obtain capsule of weeping forsythia flower extract.The content of general flavone is 12.7% (general flavone content in capsule of weeping forsythia flower extract
Calculated with rutin equivalent).
In vitro test of the capsule of weeping forsythia flower extract of embodiment 3 to the inhibitory action of tyrosinase
The capsule of weeping forsythia flower extract prepared by embodiment 1 observes the inhibitory action to tyrosinase
Tyrosinase inhibition test system is as described in Table 1.During experiment, first add Mushroom Tyrosinase (TYR) and difference
The capsule of weeping forsythia flower extract and ursin solution of concentration, 10min is preheated at 37 DEG C, is eventually adding the μ L of substrate TYR 20, is triggered
Reaction, setting Detection wavelength is 475nm, absorbance during record 30min.Every group of experiment is in triplicate.
Tyrosinase inhibition rate computing formula is：I%={ [(A-B)-(C-D)]/(A-B) } × 100%
Capsule of weeping forsythia flower extract is shown in Table 2 to the histamine result of Mushroom Tyrosinase.Illustrate that the Flos Forsythiae of present invention acquisition is extracted
Thing is close with ursin to the inhibition of Mushroom Tyrosinase activity, with stronger inhibition.
The tyrosinase inhibitory activity of table 1 tests reaction system
The IC50 that the capsule of weeping forsythia flower extract of table 2 suppresses to Mushroom Tyrosinase
Claims (7)
1. a kind of preparation method for suppressing the capsule of weeping forsythia flower extract of tyrosinase, it is characterised in that comprise the following steps：
(1) extract：Weigh a certain amount of capsule of weeping forsythia dried flower, crush, add 8~10 times of hydrophilic solvents of amount, be standing and soak for 30min,
1~2h of refluxing extraction, filtering, residue with 8~10 times of hydrophilic solvent refluxing extraction 1h of amount, is filtered again, and merging is extracted twice
Liquid, 60 DEG C are concentrated under reduced pressure into the Flos Forsythiae concentrate that proportion is 1.05~1.10；
(2) separate：Concentrate is washed with deionized water de- 2~4 first further with non-polar column chromatographic material chromatography
Column volume, then with 20%, 40%, 60% ethanol water successively respectively elute 2~4 column volumes, collect 60% ethanol water
The eluent of solution, is concentrated under reduced pressure, dries, and obtains capsule of weeping forsythia flower extract.
2. a kind of preparation method for suppressing the capsule of weeping forsythia flower extract of tyrosinase as claimed in claim 1, its feature exists
In the hydrophilic solvent described in step (1) is water, ethanol, methyl alcohol, ethanol water or methanol aqueous solution.
3. a kind of preparation method for suppressing the capsule of weeping forsythia flower extract of tyrosinase as claimed in claim 1, its feature exists
In the ethanol water is 70% ethanol water.
4. a kind of preparation method for suppressing the capsule of weeping forsythia flower extract of tyrosinase as claimed in claim 1, its feature exists
In the non-polar column chromatographic material described in step (2) can be C18Or MDS-5.
5. such as the capsule of weeping forsythia flower extract of claim 1-4 either method preparation.
6. application of the capsule of weeping forsythia flower extract as claimed in claim 5 in nti-freckle, skin-lightening cosmetic is prepared.
7. a kind of nti-freckle, skin-lightening cosmetic, contain capsule of weeping forsythia flower extract as claimed in claim 5 in its component.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710003219.0A CN106691937A (en) | 2017-01-03 | 2017-01-03 | Forsythia flower extract for inhibiting tyrosinase, and preparation method thereof and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710003219.0A CN106691937A (en) | 2017-01-03 | 2017-01-03 | Forsythia flower extract for inhibiting tyrosinase, and preparation method thereof and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106691937A true CN106691937A (en) | 2017-05-24 |
Family
ID=58903803
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710003219.0A Pending CN106691937A (en) | 2017-01-03 | 2017-01-03 | Forsythia flower extract for inhibiting tyrosinase, and preparation method thereof and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106691937A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023129732A1 (en) * | 2021-12-31 | 2023-07-06 | Laboratory Corporation Of America Holdings | Methods and systems for hydrophilic-phase extraction |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103193836A (en) * | 2013-04-19 | 2013-07-10 | 山东农业大学 | Method for separating and purifying monomers in forsythia suspensa flower |
| CN106053704A (en) * | 2016-05-09 | 2016-10-26 | 山东农业大学 | A rapid analysis method for a fructus forsythiae flower chemical component fingerprint |
-
2017
- 2017-01-03 CN CN201710003219.0A patent/CN106691937A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103193836A (en) * | 2013-04-19 | 2013-07-10 | 山东农业大学 | Method for separating and purifying monomers in forsythia suspensa flower |
| CN106053704A (en) * | 2016-05-09 | 2016-10-26 | 山东农业大学 | A rapid analysis method for a fructus forsythiae flower chemical component fingerprint |
Non-Patent Citations (7)
| Title |
|---|
| 思培峰: "连翘中连翘苷含量测定及其指纹图谱研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 朴香兰: "连翘对酪氨酸酶的抑制活性", 《中央民族大学学报（自然科学版）》 * |
| 李丹凤: "连翘花抑制酪氨酸酶活性及化学成分研究", 《山西大学 2016届硕士学位论文》 * |
| 李艳芝等: "连翘花中总黄酮提取工艺的优化", 《中国医院药学杂志》 * |
| 樊雪艳: "连翘花美白活性有效部位的筛选及其成分分析", 《化学研究与应用》 * |
| 胡芳名等: "《中国主要经济树种栽培与利用》", 31 January 2006, 中国林业出版社 * |
| 齐向东等: "《微创美容外科学》", 28 February 2013, 浙江科学技术出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023129732A1 (en) * | 2021-12-31 | 2023-07-06 | Laboratory Corporation Of America Holdings | Methods and systems for hydrophilic-phase extraction |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103211732B (en) | Preparation method and application of mixture with tyrosinase inhibitory activity | |
| CN106008172B (en) | Preparation method and application of effective part with tyrosinase inhibition effect in mulberry twigs | |
| CN103342687B (en) | Compounds with tyrosinase inhibitory activity and preparation and application thereof | |
| CN104940049B (en) | Extracting method, extract and the application of yacon plant extracts | |
| CN113264912B (en) | Method for extracting 2- (2-phenethyl) chromone component from agilawood and application thereof | |
| CN108186474B (en) | Total flavone extract of Loranthus parasiticus of Loranthus, preparation method and application | |
| KR20190087199A (en) | Cosmetic composition including extracts of sargassum horneri and enteromorpha prolifera | |
| CN102961280A (en) | Application of coreopsis tinctoria active part in preparation of anti-ageing cosmetic | |
| CN106691937A (en) | Forsythia flower extract for inhibiting tyrosinase, and preparation method thereof and application | |
| CN109316422A (en) | A kind of vegetalitas anti-sensitizer stoste and its preparation method and application | |
| CN103497091B (en) | Compound with tyrosinase inhibitory activity and preparation method thereof | |
| KR101813606B1 (en) | Cosmetic composition for skin whitening containing extracts of Indian natural substances | |
| KR100640246B1 (en) | Fermented product, new compound obtained therefrom, and skin whitening products containing the same | |
| CN105348333A (en) | Allyl phenol bioside compound and preparation method and application thereof | |
| CN104784077A (en) | Active site of viola tianschanica and application of active site in preparing whitening and beautifying cosmetics | |
| CN108403532A (en) | A kind of skin conditioner including ginseng extract | |
| KR20050092313A (en) | Skin whitening cosmetic containing a herb extract with inhibitory activity of melanin formation | |
| CN104224625A (en) | Application of polygonum capitatum extract in whitening product, and whitening product | |
| CN110105185A (en) | A kind of skin care compound and its preparation method and application and skin care item | |
| JP2002029992A (en) | Activity accelerator for active oxygen scavenging enzyme | |
| KR20200096349A (en) | Composition of Anti-Oxidant Cosmetics Using Energy Saving Molecular Cluster Supercritical Centella Asiatica Extract | |
| KR100482695B1 (en) | Composition for skin whitening containing extract from Melia azedarach or β-carboline alkaloids | |
| CN1328274C (en) | Method for continuous extracting chlorophyll and isoflavone from haulm of liquorice and application | |
| KR20030004501A (en) | Cosmetic for skin whitening containing a herb extract with inhibitory activity of melanin formation | |
| KR102366234B1 (en) | Skin wrinkle improvement effects of solvent extracts from Desmodium sequax Wall or fractions thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170524 |
|
| RJ01 | Rejection of invention patent application after publication |