CN106666335A - Method for efficiently digesting antinutritional factors and antigen proteins in animal-breeding soybean meal by acetic acid method - Google Patents
Method for efficiently digesting antinutritional factors and antigen proteins in animal-breeding soybean meal by acetic acid method Download PDFInfo
- Publication number
- CN106666335A CN106666335A CN201611270712.0A CN201611270712A CN106666335A CN 106666335 A CN106666335 A CN 106666335A CN 201611270712 A CN201611270712 A CN 201611270712A CN 106666335 A CN106666335 A CN 106666335A
- Authority
- CN
- China
- Prior art keywords
- acetic acid
- soybean meal
- bean cake
- animal
- antigen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Beans For Foods Or Fodder (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for efficiently digesting antinutritional factors and antigen proteins in animal-breeding soybean meal by an acetic acid method. The soybean meal is the most widely-used plant protein raw material in the feed industry, but a variety of antinutritional factors and antigen proteins exist in the soybean meal, and must be destroyed by certain processing before the soybean meal is used for breeding animals. According to the method, acetic acid with a certain concentration is used for rapidly treating the soybean meal under a heating condition, so that two typical antigen proteins, namely glycinin and beta-conglycinin, can be completely digested, the content of trypsin inhibiting factors is reduced from 9.5mg/g to 0.3mg/g, and the digestion rate reaches 96% or above. By the method, an effective, simple, convenient and rapid production technology is provided for significantly increasing the nutritional value of the soybean meal and improving the breeding efficiency.
Description
First, technical field
The present invention relates to Feed Manufacturing, animal cultivation and Animal nutrition field, more particularly to a kind of method of acetic acid efficient digestion
The method of animal cultivation antinutritional factor and antigen protein in bean cake.
2nd, background technology
Bean cake is the side-product obtained after the extracted Oleum Glycines of Semen sojae atricolor, and rich in various nutrient substance, wherein protein content is
40%~50%, fat is 1%~2%, and carbohydrate is about 10%~15%, also several mineral materials, vitamin etc., battalion
Foster comparison of ingredients is complete and equilibrium, is most widely used vegetable protein feed raw material.But exist in bean cake various
Antinutritional factor such as trypsin ihhibitor, antigen protein etc..Topmost trypsin ihhibitor in antinutritional factor
Inhibitory action is played to some digestive enzyme in animal body, with nutrient substance complexation into stodgy composition so that the digestion of bean cake
The absorbance of rate and animal declines；Two kinds of typical antigen proteins present in Semen sojae atricolor, glycinin and β-companion's Semen sojae atricolor ball egg
There is in vain antigenicity and sensitization, stimulating immune system produces antibody, causing allergy to be sent out should.Therefore, can exist as feedstuff
The digestibility and biological value problem poor compared with animal proteins such as fish flour.
To solve this problem, fermentable process bean cake becomes one of study hotspot in recent years, the micro- lifes of bean cake Jing
After thing fermentation, antinutritional factor activity, degradation antigen albumen can be effectively passivated, but affect the factor of fermentation a lot, it is different
Fermented bacterium, fermentation condition, final ferment effect is also not quite similar, and fermentation condition is harsh, operates relatively complicated, fermentation
Time is long, causes to commercially produce high cost.And acetic acid high-temperature catalytic bean cake is adopted, during efficient digestion bean cake not only can be reached
The purpose of antinutritional factor and antigen protein, can also shorten the time, and acetic acid recoverable, greatly save production cost.
3rd, the content of the invention
Goal of the invention：For there is various antinutritional factor and antigen protein in bean cake, research is found that employing
Certain density acetic acid quick processed soybeans bean cake in a heated condition, can efficiently clear up affect in bean cake animal cultivation
Antinutritional factor and antigen protein, to reach purpose that is quick, easily improving bean cake nutritional quality and value.
Technical scheme：In order to realize foregoing invention purpose, the technical solution used in the present invention is as follows：
1st, in a kind of method of acetic acid efficient digestion bean cake animal cultivation antinutritional factor and antigen protein method, its feature exists
In, including below step：
(1) mass concentration is added to be 5%~20% acetum (volume ratio 1: 3.5~5) and mix equal in bean material
After even, processing under 120~150 DEG C of heating conditions must process material in 10~30 minutes.
(2) it is evaporated, condensed recovery with Acetic Acid-Water azeotropic mixture contained in being evaporated under reduced pressure to processing material
More than 92% acetic acid, adds appropriate powder lime carbonate to be neutralized to material pH4.5~6.0, the bean after being cleared up after drying
Dregs of rice product.
2nd, animal cultivation antinutritional factor and antigen egg in a kind of method of acetic acid efficient digestion bean cake according to claim 1
White method, it is characterised in that：Bean cake refers to the side-product obtained after the extracted Oleum Glycines of Semen sojae atricolor, containing various antinutritional factor and
Antigen protein；Acetic acid refers to alimentary acetic acid.
3rd, animal cultivation antinutritional factor and antigen egg in a kind of method of acetic acid efficient digestion bean cake according to claim 1
White method, it is characterised in that：The mass concentration of acetum is 5%~20%, and bean cake is 1 with the solid-to-liquid ratio of acetum:
3.5~5.0, the reaction temperature that acetic acid is cleared up is 120~150 DEG C；Response time is 10~30 minutes.
4th, animal cultivation antinutritional factor and antigen egg in a kind of method of acetic acid efficient digestion bean cake according to claim 1
White method, it is characterised in that：Benzoyl-L-arginine-paranitroanilinum (L-BAPA) is used for trypsin ihhibitor
Detection；SDS-PAGE is used for the detection of two kinds of antigen proteins.
Beneficial effect：The present invention adopts certain density acetic acid quick processed soybeans bean cake in a heated condition, can be with height
Effect ground clear up in bean cake affect animal cultivation antinutritional factor and antigen protein, with reach it is quick, efficient, easily improve bean
The target of dregs of rice nutritional quality and value.
4th, specific embodiment
Embodiment 1
40g bean cake (dry weight) is added in 1000mL rustless steel tank reactors, is added by 1: 5 ratio of solid-to-liquid ratio
10% acetic acid, in being placed in electric heating constant temperature oil groove, at 120 DEG C 30min is catalyzed.Obtain the bean cake after acetic acid catalysis after drying to produce
Product.Jing SDS-PAGE detections find that glycinin and beta-conglycinin are cleared up completely；Meanwhile, trypsin suppress because
Son is reduced to 0.341mg/g by 9.504mg/g, reduces 96.41%.
Embodiment 2
1kg bean cake (dry weight) is added in 15L rotary type cookers, by 1: 5 ratio of solid-to-liquid ratio 10% acetic acid is added,
10min is catalyzed at 150 DEG C, process material is obtained final product.Entered with Acetic Acid-Water azeotropic mixture contained in being evaporated under reduced pressure to processing material
Row is evaporated, condenses the acetic acid for reclaiming more than 92%, adds appropriate powder lime carbonate to be neutralized to material pH4.5~6.0, dries
Soybean products after being cleared up afterwards.Jing SDS-PAGE detections find that glycinin and beta-conglycinin disappear completely
Solution；Meanwhile, trypsin ihhibitor is reduced to 0.309mg/g by 9.504mg/g, reduces 96.75%.
Claims (4)
1. in a kind of method of acetic acid efficient digestion bean cake animal cultivation antinutritional factor and antigen protein method, it is characterised in that
Including below step：
(1) add in bean material after the acetum of mass concentration 5%~20% (volume ratio 1: 3.5~5) and mix homogeneously, in
Processing under 120~150 DEG C of heating conditions must process material in 10~30 minutes.
(2) be evaporated with contained Acetic Acid-Water azeotropic mixture in being evaporated under reduced pressure to processing material, condense recovery 92% with
On acetic acid, add appropriate powder lime carbonate to be neutralized to material pH4.5~6.0, the soybean products after being cleared up after drying.
2. animal cultivation antinutritional factor and antigen protein in a kind of method of acetic acid efficient digestion bean cake according to claim 1
Method, it is characterised in that：Bean cake refers to the side-product obtained after the extracted Oleum Glycines of Semen sojae atricolor, containing various antinutritional factor and antigen
Albumen；Acetic acid refers to alimentary acetic acid.
3. animal cultivation antinutritional factor and antigen protein in a kind of method of acetic acid efficient digestion bean cake according to claim 1
Method, it is characterised in that：The mass concentration of acetum is 5%~20%, the solid-to-liquid ratio of bean cake and acetum is 1: 3.5~
5.0, the reaction temperature that acetic acid is cleared up is 120~150 DEG C；Response time is 10~30 minutes.
4. animal cultivation antinutritional factor and antigen protein in a kind of method of acetic acid efficient digestion bean cake according to claim 1
Method, it is characterised in that：Benzoyl-L-arginine-paranitroanilinum (L-BAPA) is used for the detection of trypsin ihhibitor；
SDS-PAGE is used for the detection of two kinds of antigen proteins.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611270712.0A CN106666335A (en) | 2016-12-28 | 2016-12-28 | Method for efficiently digesting antinutritional factors and antigen proteins in animal-breeding soybean meal by acetic acid method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611270712.0A CN106666335A (en) | 2016-12-28 | 2016-12-28 | Method for efficiently digesting antinutritional factors and antigen proteins in animal-breeding soybean meal by acetic acid method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106666335A true CN106666335A (en) | 2017-05-17 |
Family
ID=58849679
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611270712.0A Pending CN106666335A (en) | 2016-12-28 | 2016-12-28 | Method for efficiently digesting antinutritional factors and antigen proteins in animal-breeding soybean meal by acetic acid method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106666335A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108576397A (en) * | 2018-05-29 | 2018-09-28 | 南京林业大学 | A kind of method that anti-nutritional factors in dregs of beans is efficiently cut down in ferrous ion collaboration acetic acid catalysis |
| CN108835422A (en) * | 2018-07-28 | 2018-11-20 | 湖南民菌生态农业有限公司 | The preparation method of one boar food |
| CN113785933A (en) * | 2021-09-16 | 2021-12-14 | 陕西理工大学 | Method for eliminating activity of antinutritional factor serine protease inhibitor in folium Mori and its application |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1555719A (en) * | 2003-12-30 | 2004-12-22 | 华中农业大学 | Method of eliminating antinutrition fuctor in soya dreg using fermentation method |
| CN102511746A (en) * | 2011-12-26 | 2012-06-27 | 广西南宁市康之豆食品科技有限责任公司 | Detoxification curing technology for whole-grain soybeans |
| CN102907627A (en) * | 2012-11-16 | 2013-02-06 | 桂仕林 | Preparation technique and application of non-odor soybean |
| US20140050830A1 (en) * | 2011-02-24 | 2014-02-20 | Grovac Systems International L.C. | Methods of processing raw foods and related food products |
| CN103621774A (en) * | 2013-09-05 | 2014-03-12 | 深圳比利美英伟营养饲料有限公司 | Soybeans without anti-nutritional factors and method for removing anti-nutritional factors of soybeans as well as application of soybeans |
| CN104798891A (en) * | 2015-04-30 | 2015-07-29 | 贵州大学 | Salting-out and adsorption processing method for low-purine soybean milk |
| CN104905085A (en) * | 2015-05-29 | 2015-09-16 | 沈阳康利生物科技有限公司 | Method for activating enzyme of whole grains of soybeans |
-
2016
- 2016-12-28 CN CN201611270712.0A patent/CN106666335A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1555719A (en) * | 2003-12-30 | 2004-12-22 | 华中农业大学 | Method of eliminating antinutrition fuctor in soya dreg using fermentation method |
| US20140050830A1 (en) * | 2011-02-24 | 2014-02-20 | Grovac Systems International L.C. | Methods of processing raw foods and related food products |
| CN102511746A (en) * | 2011-12-26 | 2012-06-27 | 广西南宁市康之豆食品科技有限责任公司 | Detoxification curing technology for whole-grain soybeans |
| CN102907627A (en) * | 2012-11-16 | 2013-02-06 | 桂仕林 | Preparation technique and application of non-odor soybean |
| CN103621774A (en) * | 2013-09-05 | 2014-03-12 | 深圳比利美英伟营养饲料有限公司 | Soybeans without anti-nutritional factors and method for removing anti-nutritional factors of soybeans as well as application of soybeans |
| CN104798891A (en) * | 2015-04-30 | 2015-07-29 | 贵州大学 | Salting-out and adsorption processing method for low-purine soybean milk |
| CN104905085A (en) * | 2015-05-29 | 2015-09-16 | 沈阳康利生物科技有限公司 | Method for activating enzyme of whole grains of soybeans |
Non-Patent Citations (2)
| Title |
|---|
| 单安山: "《饲料配制大全》", 31 July 2005, 北京：中国农业出版社 * |
| 汪儆: "《饲料毒物与抗营养因子研究进展》", 31 October 1997, 西北大学出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108576397A (en) * | 2018-05-29 | 2018-09-28 | 南京林业大学 | A kind of method that anti-nutritional factors in dregs of beans is efficiently cut down in ferrous ion collaboration acetic acid catalysis |
| CN108835422A (en) * | 2018-07-28 | 2018-11-20 | 湖南民菌生态农业有限公司 | The preparation method of one boar food |
| CN113785933A (en) * | 2021-09-16 | 2021-12-14 | 陕西理工大学 | Method for eliminating activity of antinutritional factor serine protease inhibitor in folium Mori and its application |
| CN113785933B (en) * | 2021-09-16 | 2022-05-03 | 陕西理工大学 | Method for eliminating activity of anti-nutritional factor serine protease inhibitor in mulberry leaves and application of anti-nutritional factor serine protease inhibitor in mulberry leaves |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106615672B (en) | High-quality enzymolysis fermented soybean meal and preparation method and application thereof | |
| CN101773203A (en) | Method for biologically improving oilseed residue | |
| CN101019623A (en) | Making process of fermented plant fiber and protein food | |
| CN102783581A (en) | Preparation method of piglet feed fermented by tomato, hawthorn and fish meal | |
| CN104041817A (en) | Method for preparing longan ferment from longan kernel | |
| CN107557420A (en) | A kind of preparation method of flaxseed meal polypeptide | |
| CN106993807A (en) | A kind of preparation method of ginger ferment | |
| CN104115991A (en) | Vinasse fermentation forage edible by beef cattle | |
| CN108077654A (en) | A kind of fish cold granulation feed and preparation method thereof that ferments | |
| CN106666335A (en) | Method for efficiently digesting antinutritional factors and antigen proteins in animal-breeding soybean meal by acetic acid method | |
| CN101390567A (en) | Production method of DDS biology protein feedstuff | |
| CN103276038B (en) | Method for producing reagent grade peptone | |
| CN106804958A (en) | It is a kind of to improve piglet digestive function and improve liquid feed of feed intake and preparation method thereof | |
| CN104012945B (en) | The preparation method of solid Germinatus Phragmitis ferment | |
| CN106721292A (en) | A kind of milking sow dedicated liquid feed for improving milk quality and preparation method thereof | |
| CN103224970B (en) | Feeding plant protein peptide preparation method | |
| CN105851625A (en) | Feed for promoting growth and egg laying of hens and preparation method thereof | |
| CN102277403A (en) | Production technology of yellow wine lees proteins by enzymatic extraction | |
| CN104621423A (en) | Physical detoxification method for cottonseed cake meal | |
| CN103907684B (en) | A kind of composite high-protein liquid and preparation method thereof, purposes | |
| CN101601438A (en) | A kind of dregs of beans quality modification technique | |
| TWI797491B (en) | Method for hydrolyzing soybean protein by fermenting lactic acid bacteria | |
| CN106689741A (en) | Liquid feed for alleviating pig heat stress caused by high temperature climate and preparation method thereof | |
| CN108477395A (en) | A kind of production method of feed additive for ruminant | |
| RU2336723C1 (en) | Method of manufacturing concentrated fodder based on soybean seeds |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |