CN106665388A - Empathy testing device and method - Google Patents
Empathy testing device and method Download PDFInfo
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- CN106665388A CN106665388A CN201611218183.XA CN201611218183A CN106665388A CN 106665388 A CN106665388 A CN 106665388A CN 201611218183 A CN201611218183 A CN 201611218183A CN 106665388 A CN106665388 A CN 106665388A
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- 238000012360 testing method Methods 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000010171 animal model Methods 0.000 claims abstract description 164
- 238000012544 monitoring process Methods 0.000 claims abstract description 28
- 238000010191 image analysis Methods 0.000 claims abstract description 23
- 238000004891 communication Methods 0.000 claims abstract description 7
- 241001465754 Metazoa Species 0.000 claims description 182
- 238000004458 analytical method Methods 0.000 claims description 23
- 230000008520 organization Effects 0.000 claims description 13
- 230000008859 change Effects 0.000 claims description 10
- 230000005693 optoelectronics Effects 0.000 claims description 8
- 239000013307 optical fiber Substances 0.000 claims description 7
- 230000035479 physiological effects, processes and functions Effects 0.000 claims description 6
- 238000010998 test method Methods 0.000 claims description 5
- 241000894007 species Species 0.000 claims description 4
- 230000005540 biological transmission Effects 0.000 claims description 3
- 230000007246 mechanism Effects 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 230000035807 sensation Effects 0.000 claims description 3
- 238000012806 monitoring device Methods 0.000 claims 1
- 230000006399 behavior Effects 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 5
- 210000004556 brain Anatomy 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 230000001149 cognitive effect Effects 0.000 description 4
- 210000005036 nerve Anatomy 0.000 description 4
- 241000699670 Mus sp. Species 0.000 description 3
- 230000002490 cerebral effect Effects 0.000 description 3
- 230000002996 emotional effect Effects 0.000 description 3
- 230000006397 emotional response Effects 0.000 description 3
- 230000001537 neural effect Effects 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 229960000583 acetic acid Drugs 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000003973 paint Substances 0.000 description 2
- 230000003187 abdominal effect Effects 0.000 description 1
- 238000004378 air conditioning Methods 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 230000005622 photoelectricity Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
- A01K1/031—Cages for laboratory animals; Cages for measuring metabolism of animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K29/00—Other apparatus for animal husbandry
- A01K29/005—Monitoring or measuring activity, e.g. detecting heat or mating
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Abstract
The embodiment of the invention provides an empathy testing device and method. The device comprises a low temperature region bin body, a warm region bin body, temperature regulation equipment, video monitoring equipment and control equipment, the low temperature region bin body and the warm region bin body are both connected with the temperature regulation equipment, the low temperature region bin body is communicated with the warm region bin body, the video monitoring equipment collects images of experimental animals in the warm region bin body and analyzes the collected images, and image analysis results are obtained; the temperature regulation equipment is in communication connection with the control equipment which controls the temperature in the low temperature region bin body and the warm region bin body through the temperature regulation equipment, the video monitoring equipment is in communication connection with the control equipment, and the control equipment obtains the image analysis results obtained through the video monitoring equipment. According to the empathy testing device and method, empathy of the experimental animals can be tested through expression of the experimental animals crowded in the small warm region bin body, the empathy of the experimental animals can be tested conveniently, and the requirement of the empathy test is met.
Description
Technical field
The present invention relates to altogether feelings technical field of measurement and test, more particularly to feelings test device and method altogether.
Background technology
Common feelings in animals are one and have the topic disputed on, often by feelings and feelings altogether in the research of neuroscience
Thread infection is even imitated and mutually obscured.The emotional response that common feelings are defined as other companions can excite phase with target animal
As emotional response process, but this definition is Relative Fuzzy, only the angle of subordinate act may cannot be distinguished by out
Common feelings, imitation and emotional contagion, because also having a kind of phenomenon equally to replicate the behavior expression of companion, this phenomenon in animal
It is called imitation.And it is the infection for passively receiving companion's emotional response between common feelings and imitation that emotional contagion is then.
A kind of research approach is glacial acetic acid injection experiment, and the test is by carrying out the ice of abdominal part to being chosen as victim mice
Acetic acid injection makes it produce pain and frightened behavior reaction, allows witness mice to observe, right as testing using witness mice
As observing it for companion is reacted to carry out studying for common feelings by pain.
But this research approach effectively cannot distinguish feelings altogether with imitation, emotional contagion, it is impossible to fully meet common feelings
Probe into.
The content of the invention
The purpose of the embodiment of the present invention is to provide a kind of feelings test device and method altogether, deeply to be ground to common feelings
Study carefully.Concrete technical scheme is as follows：
A kind of feelings test device altogether, including：Low-temperature space warehouse, warmer region warehouse, temperature control equipment, video monitoring equipment
And control device,
The low-temperature space warehouse and the warmer region warehouse are connected with the temperature control equipment, the low-temperature space warehouse
It is connected with the warmer region warehouse, image of the video monitoring equipment collection laboratory animal in the warmer region warehouse is simultaneously
Image to gathering is analyzed, and obtains image analysis result；Wherein, the size of the warmer region warehouse and a laboratory animal
Size match, the low-temperature space warehouse be more than the warmer region warehouse；
The temperature control equipment is communicated to connect with the control device, and the control device is set by the temperature adjustment
It is standby to control the low-temperature space warehouse and the temperature in the warehouse of warmer region, the video monitoring equipment and the control device communication link
Connect, the control device obtains the image analysis result that the video monitoring equipment is obtained.
Optionally, the control device controls temperature in the low-temperature space warehouse for the by the temperature control equipment
One temperature, it is second temperature that the control device controls the temperature in the warmer region warehouse by the temperature control equipment,
The second temperature is higher than first temperature；
The image analysis result obtained in the control device includes：Laboratory animal quantity in the warmer region warehouse is sent out
During changing, the temperature in the warmer region warehouse is accordingly adjusted by the temperature control equipment, when the warmer region warehouse
In laboratory animal quantity increase when, by the temperature control equipment by the warmer region warehouse temperature reduce A degree, its
The number * C of the laboratory animal increased in the warehouse of middle A=warmer regions, C are for preset temperature value and C is less than the second temperature；Work as institute
When stating laboratory animal quantity in the warehouse of warmer region and reducing, by the temperature control equipment by the temperature in the warmer region warehouse
Degree raises B degree, the number * C of the laboratory animal reduced wherein in the warehouse of B=warmer regions.
Optionally, also include：The light regulation device communicated to connect with the control device, the smooth regulation device includes：
Laser instrument, waveform generator, optical fiber and implantable fine setting optoelectronic pole, the waveform generator is connected with laser instrument, described to swash
The laser output of light device is finely tuned optoelectronic pole and is connected by the optical fiber and the implantable, and the implantable finely tunes photoelectricity
Pole is arranged on the big intracerebral of laboratory animal for having proceeded to light sensation gene.
Optionally, described device also includes：With the electrical signal collection device of control device communication connection, the telecommunications
Number harvester includes：Microelectrode and electrical signal collection device, the microelectrode is arranged on the big intracerebral of laboratory animal, the electricity
Signal picker is communicated to connect with the microelectrode, receives the signal of telecommunication of the microelectrode transmission and the signal of telecommunication is entered
Row analysis, obtains signal of telecommunication analysis result.
Optionally, the video monitoring equipment is analyzed to the image for gathering, and obtains being tested in the warmer region warehouse
The number of animal data over time, the delta data is added in image analysis result；The control device according to
Temperature and the smooth regulation device in described image analysis result, the signal of telecommunication analysis result, the warmer region warehouse is sent out
The laser for going out, it is determined that feelings test result altogether.
Optionally, the low-temperature space warehouse and the warmer region warehouse are behavioristicss' casing.
Optionally, also include：Dodge gate and controlling organization, the dodge gate is arranged at the low-temperature space warehouse and the temperature
Between warm area's warehouse, the dodge gate is connected with the controlling organization, is opened and closed by controlling organization control, the control
Mechanism processed communicates to connect with the control device.
Optionally, the video monitoring equipment includes：The photographic head being connected and video analysis device.
Optionally, can also include：Physiology signal acquisition device, the physiology signal acquisition device collection laboratory animal
The physiological signal and physiological signal to being gathered is analyzed, obtains physiological signal analysis result.
A kind of feelings method of testing altogether, using above-mentioned common feelings test device common feelings test, the side are carried out to laboratory animal
Method includes：
It is the first temperature that the control device controls the temperature in the low-temperature space warehouse by the temperature control equipment,
It is second temperature that the control device controls temperature in the warmer region warehouse by the temperature control equipment, described second
Temperature is higher than first temperature；
The control device controls the first laboratory animal and stops Preset Time in the low-temperature space warehouse；
The control device controls first laboratory animal and is located in the warmer region warehouse and by multiple with described the
One laboratory animal is that the laboratory animal of similar species is placed in the low-temperature space warehouse；
The control device determines the warmer region warehouse according to the image analysis result that the video monitoring equipment is obtained
The variable quantity of middle laboratory animal number, by the temperature control equipment temperature in the warmer region warehouse is accordingly adjusted, when
When laboratory animal quantity in the warmer region warehouse increases, by the temperature control equipment by the warmer region warehouse
Temperature reduces A degree, the number * C of the laboratory animal increased wherein in the warehouse of A=warmer regions, and C is for preset temperature value and C is less than institute
State second temperature；When the laboratory animal quantity in the warmer region warehouse is reduced, will be described by the temperature control equipment
Temperature in the warehouse of warmer region raises B degree, the number * C of the laboratory animal reduced wherein in the warehouse of B=warmer regions；
Warmer region warehouse caused by temperature change and the temperature change in warmer region warehouse described in the control device
The variable quantity of middle laboratory animal number determines feelings test result altogether.
A kind of feelings test device and method altogether provided in an embodiment of the present invention, device includes：Low-temperature space warehouse, warmer region storehouse
Body, temperature control equipment, video monitoring equipment and control device, the low-temperature space warehouse and the warmer region warehouse with it is described
Temperature control equipment connects, and the low-temperature space warehouse is connected with the warmer region warehouse, and the video monitoring equipment collection is real
Test image of the animal in the warmer region warehouse and the image to gathering is analyzed, obtain image analysis result；Wherein, institute
The size and the size of a laboratory animal for stating warmer region warehouse matches, and the low-temperature space warehouse is more than the warmer region storehouse
Body；The temperature control equipment is communicated to connect with the control device, and the control device is by the temperature control equipment control
The low-temperature space warehouse and the temperature in the warehouse of warmer region are made, the video monitoring equipment is communicated to connect with the control device,
The control device obtains the image analysis result that the video monitoring equipment is obtained.The present invention can be by laboratory animal little
Warmer region warehouse in it is crowded when performance testing the common feelings of laboratory animal, the present invention can conveniently to reality
The common feelings for testing animal are tested, and meet the needs of common feelings test.
Certainly, implementing arbitrary product or method of the present invention must be not necessarily required to while reaching all the above excellent
Point.
Description of the drawings
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
The accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is a kind of structural representation of feelings test device altogether provided in an embodiment of the present invention；
Fig. 2 is the structural representation of another kind of feelings test device altogether provided in an embodiment of the present invention；
Fig. 3 is a kind of flow chart of feelings method of testing altogether provided in an embodiment of the present invention.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than the embodiment of whole.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
As shown in figure 1, a kind of feelings test device altogether provided in an embodiment of the present invention, can include：Low-temperature space warehouse 100,
Warmer region warehouse 200, temperature control equipment 300, video monitoring equipment 400 and control device 500,
The low-temperature space warehouse 100 and the warmer region warehouse 200 are connected with the temperature control equipment 300, described
Low-temperature space warehouse 100 is connected with the warmer region warehouse 200, and the video monitoring equipment 400 gathers laboratory animal described
Image in the warmer region warehouse 200 and image to gathering is analyzed, obtains image analysis result；Wherein, the warmer region
The size of warehouse 200 matches with the size of a laboratory animal, and the low-temperature space warehouse 100 is more than the warmer region warehouse
200；
The temperature control equipment 300 is communicated to connect with the control device 500, and the control device 500 is by described
Temperature control equipment 300 controls the low-temperature space warehouse 100 and the temperature in warmer region warehouse 200, the video monitoring equipment
400 communicate to connect with the control device 500, and the control device 500 obtains the image that the video monitoring equipment 400 is obtained
Analysis result.
Inventor's research finds：Altogether feelings should have higher level cerebral activity --- possessing has cognitive aspect
Activity.And decision-making is the preferably embodiment of cognitive activities.It is the instinct of animal to go after profits and advoid disadvantages, but he reacts because of profit that altogether feelings are produced
Similarly it is the characteristics of animal is universal.Therefore animal being placed between his choice egoistic or sharp can indirectly judge being total to for animal
Feelings.
Specifically, low-temperature space warehouse 100, warmer region warehouse 200 can be all-transparent warehouse or the storehouse with transparent region
Body.Specifically, low-temperature space warehouse 100, warmer region warehouse 200 can be behavioristicss' casing, and behavioristicss' casing is 20x20x40cm
Square box.
Sound and taste can be mutually isolated between low-temperature space warehouse 100, warmer region warehouse 200.Wherein, the video prison
Control equipment 400 can include：The photographic head being connected and video analysis device.Photographic head can be one or more.Wherein, depending on
Frequency division analysis apparatus can obtain image analysis result to being analyzed in the image of photographic head collection.Wherein, the table of laboratory animal
Can now have various, such as：The time of staying, time, exclusive behavioral duration, exclusive behavior expression form that exclusive behavior occur
Deng.Wherein, exclusive behavior expression form can have various, such as：Other laboratory animals are baited, is kicked and is pushed away other laboratory animals, Xiang Qi
His laboratory animal sends threat sound etc..
Wherein, temperature control equipment 300 can be air-conditioning, and low-temperature space warehouse and the warmer region warehouse can pass through pipeline
It is connected with air-conditioner air outlet.
Can be directly connected between each warehouse, it is also possible to connected by passage, here of the present invention is not limited.On each warehouse
Need to arrange corresponding hole so that laboratory animal can be with the into/out warehouse.
Optionally, brightness regulation equipment is also provided with each warehouse, to adjust the brightness in each warehouse.Wherein, it is bright
Degree adjustment equipment can be intensity adjustable luminaire.
Wherein, Fig. 1 shown devices can also include：Dodge gate and controlling organization,
The dodge gate is arranged between the low-temperature space warehouse and the warmer region warehouse, the dodge gate and the control
Mechanism's connection processed, is opened and closed by controlling organization control, and the controlling organization is communicated to connect with the control device.It is logical
Crossing dodge gate just can facilitate in any one that Control release animal is located in the two warehouses.
Wherein, laboratory animal can be mouse.In actual applications, can wear with different laboratory animals/glue
The different mark of patch, the such as different paint of color, to make a distinction to each laboratory animal.
Specifically, the control device can control the temperature in the low-temperature space warehouse by the temperature control equipment
For the first temperature, it is second warm that the control device controls the temperature in the warmer region warehouse by the temperature control equipment
Degree, the second temperature is higher than first temperature；
The image analysis result obtained in the control device includes：Laboratory animal quantity in the warmer region warehouse is sent out
During changing, the temperature in the warmer region warehouse is accordingly adjusted by the temperature control equipment.
When the laboratory animal quantity in the warmer region warehouse increases, will be described warm by the temperature control equipment
Temperature in area's warehouse reduces A degree, wherein, the number * C of the laboratory animal increased in the warehouse of A=warmer regions, C is preset temperature
It is worth and C is less than the second temperature；
When the laboratory animal quantity in the warmer region warehouse is reduced, will be described warm by the temperature control equipment
Temperature in area's warehouse raises B degree, wherein, the number * C of the laboratory animal reduced in the warehouse of B=warmer regions.
By this process, promote laboratory animal to make a choice, be that " continuation is together with other laboratory animals in warmer region
But temperature is reduced ", or " driving other laboratory animals out of warmer region to elevate the temperature ", so as to showing according to laboratory animal
To common feelings test result.
Specifically, in actual applications, the common feelings of laboratory animal can be tested in accordance with the following steps：
Step one, the control device control temperature in the low-temperature space warehouse for the by the temperature control equipment
One temperature, it is second temperature that the control device controls the temperature in the warmer region warehouse by the temperature control equipment,
The second temperature is higher than first temperature；
Step 2, the control device control the first laboratory animal and stop Preset Time in the low-temperature space warehouse；
Preset Time is stopped in the low-temperature space warehouse by controlling the first laboratory animal, the first laboratory animal is allowed for
Know the temperature property of low-temperature space warehouse, produce sense of discomfort.When other laboratory animals enter warmer region warehouse by low-temperature space warehouse
When, the first laboratory animal is known that other laboratory animals are to come more comfortable environment from an environment for having sense of discomfort.
First laboratory animal is also known that other laboratory animals are expelled into warmer region warehouse means that other laboratory animals will be returned to
The environment of sense of discomfort.
Step 3, the control device control first laboratory animal be located in the warmer region warehouse and by multiple and
First laboratory animal is that the laboratory animal of similar species is placed in the low-temperature space warehouse；
Step 4, the control device determine described warm according to the image analysis result that the video monitoring equipment is obtained
The variable quantity of laboratory animal number in area's warehouse, by the temperature control equipment temperature in the warmer region warehouse is accordingly adjusted
Degree, when the laboratory animal quantity in the warmer region warehouse increases, by the temperature control equipment by the warmer region storehouse
Temperature in body reduces A degree, the number * C of the laboratory animal increased wherein in the warehouse of A=warmer regions, and C is preset temperature value and C
Less than the second temperature；When the laboratory animal quantity in the warmer region warehouse is reduced, by the temperature control equipment
Temperature in the warmer region warehouse is raised into B degree, the number * C of the laboratory animal reduced wherein in the warehouse of B=warmer regions；
In actual applications, C can be 4 DEG C, and the first temperature can be 12 DEG C, and second temperature can be 24 DEG C.Preferably,
The temperature of warmer region warehouse is minimum can be down to identical with the temperature of low-temperature space warehouse, and the temperature highest of warmer region warehouse is
Two temperature.
By the control of said temperature, it is possible to so that laboratory animal recognizes that laboratory animal is more in the warehouse of warmer region,
Then temperature is lower, and environment is more severe；Laboratory animal is fewer in the warehouse of warmer region, then temperature is higher, and environment is more comfortable.Work as warmer region
Laboratory animal is fewer in warehouse, then more other laboratory animals will be in low-temperature space warehouse.So, the first laboratory animal permits
Perhaps other laboratory animals are stopped in the warehouse of warmer region duration, the number of other laboratory animals of the first laboratory animal permission stop
Temperature of warmer region warehouse etc. may serve to the common feelings of evaluation experimental animal when exclusive behavior occur in amount, the first laboratory animal.
It is warm caused by temperature change and the temperature change in step 5, warmer region warehouse described in the control device
The variable quantity of laboratory animal number determines feelings test result altogether in area's warehouse.
A kind of feelings test device altogether provided in an embodiment of the present invention, including identical warmer region warehouse and low-temperature space warehouse,
Although temperature is higher in the warehouse of warmer region, size only matches with the size of a laboratory animal.When in the warehouse of warmer region
When having a laboratory animal, the laboratory animal can not be by overstocked；But other laboratory animals are necessarily attracted and are entered by temperature
Warmer region warehouse, now the multiple laboratory animals in the warehouse of warmer region will overstock mutually.When the common feelings of laboratory animal are higher, its
It is also higher to this overstocked tolerances, on the contrary it is then relatively low.Therefore the present invention can be by laboratory animal in little warmer region storehouse
Show to test the common feelings of laboratory animal when crowded in body, the present invention conveniently can be total to laboratory animal
Feelings are tested, and meet the needs of common feelings test.
Further, as shown in Fig. 2 a kind of feelings test device altogether provided in an embodiment of the present invention, can also include：With it is described
The light regulation device 600 of control device communication connection,
The smooth regulation device 600 includes：Laser instrument, waveform generator, optical fiber and implantable fine setting optoelectronic pole, it is described
Waveform generator is connected with laser instrument, and the laser output of the laser instrument finely tunes light by the optical fiber with the implantable
Electrode connects, and the implantable fine setting optoelectronic pole is arranged on the big intracerebral of laboratory animal for having proceeded to light sensation gene.
Light regulation device 600 can be stimulated laboratory animal brain by different stimulus modelities.
Specifically, it is possible to use waveform generator adjusts laser instrument and projects power, frequency, intensity of laser etc..Specifically,
Laser is communicated to implantable by optical fiber finely tunes target area of the optoelectronic pole to stimulate cerebral tissue carries out the irradiation of specific neuronal unit, comes
Realize for a certain particular core group and neural circuitry regulate and control.Meanwhile, waveform generator can also be by controlling laser instrument
Come the activity for blocking or strengthening neuron.
Further, a kind of feelings test device altogether provided in an embodiment of the present invention, can also include：It is logical with the control device
The electrical signal collection device of letter connection,
The electrical signal collection device includes：Microelectrode and electrical signal collection device, the microelectrode is arranged on experiment
In animal brain, the electrical signal collection device is communicated to connect with the microelectrode, receives the telecommunications of the microelectrode transmission
Number and the signal of telecommunication is analyzed, obtain signal of telecommunication analysis result.
Electrical signal collection device can insert specific core group or brain area to record target nerve unit by microelectrode
Active state, obtain target nerve unit activity and observation sex dread obtain behavior between dependency.
Specifically, on the one hand the present invention can carry out the stimulation of specific nerve using light regulation device 600, by observing it
The result of decision is it may determine that whether the activity of the nerve can improve or reduce the common feelings of laboratory animal.
On the other hand using the brainwave activity situation of electrical signal collection device recording laboratory animal, can be dynamic for experiment
Mental status when thing carries out decision-making is had gained some understanding, it can be appreciated that if laboratory animal selects egoistic, then be which brain
The activity in area defeats his impulsion sharp, and vice versa.
Optionally, the image of 400 pairs of collections of the video monitoring equipment is analyzed, and obtains real in the warmer region warehouse
The number of animal data over time are tested, the delta data is added in image analysis result；The control device 500
Can be adjusted according to the temperature in described image analysis result, the signal of telecommunication analysis result, the warmer region warehouse and the light
The laser that control device sends, it is determined that feelings test result altogether.
In actual applications, the first laboratory animal can be allowed other laboratory animals to enter warmer region warehouse by the present invention
Time, as the access time, will allow access into the quantity of other laboratory animals of warmer region warehouse as access number, will allow
The duration that certain other laboratory animal is stopped in warmer region warehouse is used as access duration.
Further, the present invention can be using access duration, access time, access number as X-axis, with electrical signal collection
The Neural spike train intensity that device is collected as Z axis, the electric discharge site in laboratory animal brain as Y-axis, so as to obtain profit
The generation site of the common feelings of his property.
In addition, light regulation device 600 is recycled for these sites stimulate, the access duration of observation experiment animal,
Whether access time, access number change to verify whether these sites really take part in the generation of common feelings.
Further, a kind of feelings test device altogether provided in an embodiment of the present invention, can also include：Physiological signal collection is filled
Put, the physiological signal and the physiological signal to being gathered of the physiology signal acquisition device collection laboratory animal are analyzed, and obtain
To physiological signal analysis result.
Wherein, the physiology signal acquisition device is heart rate harvester, and the physiological signal is heart rate.
The heart rate harvester can be made up of collection necklace and heart rate analysis equipment, and collection necklace is worn on laboratory animal body
On body and heart rate signal is sent to into heart rate analysis equipment by wired or wireless way to be analyzed, from determination laboratory animal
Common relation between feelings degree and heart rate signal.
The control device 500 of the present invention can be according to described image analysis result, the signal of telecommunication analysis result, the temperature
The laser that temperature and the smooth regulation device in warm area's warehouse sends, it is determined that feelings test result altogether.Due to having considered figure
As swashing that the temperature and the smooth regulation device in analysis result, the signal of telecommunication analysis result, the warmer region warehouse sends
Light, therefore the common feelings test result for drawing is more comprehensive and accurate, reliability is higher, being total to multi-analysis laboratory animal
Feelings are showed.
As shown in figure 3, present invention also offers a kind of altogether feelings method of testing, using the common feelings test device of any one above-mentioned
Common feelings test is carried out to laboratory animal, the method can include：
It is first that S100, the control device control the temperature in the low-temperature space warehouse by the temperature control equipment
Temperature, it is second temperature that the control device controls temperature in the warmer region warehouse by the temperature control equipment, institute
Second temperature is stated higher than first temperature；
S200, the control device control the first laboratory animal and stop Preset Time in the low-temperature space warehouse；
S300, the control device control first laboratory animal and are located in the warmer region warehouse and by multiple and institute
State the laboratory animal that the first laboratory animal is similar species to be placed in the low-temperature space warehouse；
S400, the control device determine the warmer region according to the image analysis result that the video monitoring equipment is obtained
The variable quantity of laboratory animal number in warehouse, by the temperature control equipment temperature in the warmer region warehouse is accordingly adjusted
Degree, when the laboratory animal quantity in the warmer region warehouse increases, by the temperature control equipment by the warmer region storehouse
Temperature in body reduces A degree, the number * C of the laboratory animal increased wherein in the warehouse of A=warmer regions, and C is preset temperature value and C
Less than the second temperature；When the laboratory animal quantity in the warmer region warehouse is reduced, by the temperature control equipment
Temperature in the warmer region warehouse is raised into B degree, the number * C of the laboratory animal reduced wherein in the warehouse of B=warmer regions；
By this process, promote laboratory animal to make a choice, be that " continuation is together with other laboratory animals in warmer region
But temperature is reduced ", or " driving other laboratory animals out of warmer region to elevate the temperature ", so as to showing according to laboratory animal
To common feelings test result.
Preset Time is stopped in the low-temperature space warehouse by controlling the first laboratory animal, the first laboratory animal is allowed for
Know the temperature property of low-temperature space warehouse, produce sense of discomfort.When other laboratory animals enter warmer region warehouse by low-temperature space warehouse
When, the first laboratory animal is known that other laboratory animals are to come more comfortable environment from an environment for having sense of discomfort.
First laboratory animal is also known that other laboratory animals are expelled into warmer region warehouse means that other laboratory animals will be returned to
The environment of sense of discomfort.
In actual applications, C can be 4 DEG C, and the first temperature can be 12 DEG C, and second temperature can be 24 DEG C.Preferably,
The temperature of warmer region warehouse is minimum can be down to identical with the temperature of low-temperature space warehouse, and the temperature highest of warmer region warehouse is
Two temperature.
By the control of said temperature, it is possible to so that laboratory animal recognizes that laboratory animal is more in the warehouse of warmer region,
Then temperature is lower, and environment is more severe；Laboratory animal is fewer in the warehouse of warmer region, then temperature is higher, and environment is more comfortable.Work as warmer region
Laboratory animal is fewer in warehouse, then more other laboratory animals will be in low-temperature space warehouse.So, the first laboratory animal permits
Perhaps other laboratory animals are stopped in the warehouse of warmer region duration, the number of other laboratory animals of the first laboratory animal permission stop
Temperature of warmer region warehouse etc. may serve to the common feelings of evaluation experimental animal when exclusive behavior occur in amount, the first laboratory animal.
Warmer region caused by temperature change and the temperature change in S500, warmer region warehouse described in the control device
The variable quantity of laboratory animal number determines feelings test result altogether in warehouse.
Inventor's research finds：Altogether feelings should have higher level cerebral activity --- possessing has cognitive aspect
Activity.And decision-making is the preferably embodiment of cognitive activities.It is the instinct of animal to go after profits and advoid disadvantages, but he reacts because of profit that altogether feelings are produced
Similarly it is the characteristics of animal is universal.Therefore animal being placed between his choice egoistic or sharp can indirectly judge being total to for animal
Feelings.
Wherein, Fig. 1 shown devices can also include：Dodge gate and controlling organization, the dodge gate is arranged at the low temperature
Between area's warehouse and the warmer region warehouse, the dodge gate is connected with the controlling organization, is opened by controlling organization control
Open and close, the controlling organization is communicated to connect with the control device.Control release animal can just be facilitated by dodge gate
In any one in the two warehouses.
Wherein, laboratory animal can be mouse.In actual applications, can wear with different laboratory animals/glue
The different mark of patch, the such as different paint of color, to make a distinction to each laboratory animal.
Specifically, the control device can control the temperature in the low-temperature space warehouse by the temperature control equipment
For the first temperature, it is second warm that the control device controls the temperature in the warmer region warehouse by the temperature control equipment
Degree, the second temperature is higher than first temperature；
A kind of feelings method of testing altogether provided in an embodiment of the present invention, can be by multiple laboratory animals in the warehouse of warmer region
Performance carry out common feelings test.Although temperature is higher in the warehouse of warmer region, size size phase only with a laboratory animal
Match somebody with somebody.When only one of which laboratory animal in the warehouse of warmer region, the laboratory animal can not be by overstocked；But other laboratory animal certainty
Attracted and into warmer region warehouse by temperature, now the multiple laboratory animals in the warehouse of warmer region will overstock mutually.Work as experiment
When the common feelings of animal are higher, it is also higher to this overstocked tolerances, otherwise then relatively low.Therefore the present invention can be by experiment
Show to test the common feelings of laboratory animal when animal is crowded in little warmer region warehouse, the present invention can be more square
Just the common feelings to laboratory animal are tested, and meet the needs of common feelings test.
It should be noted that herein, such as first and second or the like relational terms are used merely to a reality
Body or operation make a distinction with another entity or operation, and not necessarily require or imply these entities or deposit between operating
In any this actual relation or order.And, term " including ", "comprising" or its any other variant are intended to
Nonexcludability is included, so that a series of process, method, article or equipment including key elements not only will including those
Element, but also including other key elements being not expressly set out, or also include for this process, method, article or equipment
Intrinsic key element.In the absence of more restrictions, the key element for being limited by sentence "including a ...", it is not excluded that
Also there is other identical element in process, method, article or equipment including the key element.
Each embodiment in this specification is described by the way of correlation, identical similar portion between each embodiment
Divide mutually referring to what each embodiment was stressed is the difference with other embodiment.Especially for system reality
For applying example, because it is substantially similar to embodiment of the method, so description is fairly simple, related part is referring to embodiment of the method
Part explanation.
Presently preferred embodiments of the present invention is the foregoing is only, protection scope of the present invention is not intended to limit.It is all
Any modification, equivalent substitution and improvements made within the spirit and principles in the present invention etc., are all contained in protection scope of the present invention
It is interior.
Claims (10)
1. it is a kind of to be total to feelings test device, it is characterised in that to include：Low-temperature space warehouse, warmer region warehouse, temperature control equipment, regard
Frequency monitoring device and control device,
The low-temperature space warehouse and the warmer region warehouse are connected with the temperature control equipment, the low-temperature space warehouse and institute
State warmer region warehouse to be connected, image of the video monitoring equipment collection laboratory animal in the warmer region warehouse and to adopting
The image of collection is analyzed, and obtains image analysis result；Wherein, the chi of the size of the warmer region warehouse and a laboratory animal
Very little to match, the low-temperature space warehouse is more than the warmer region warehouse；
The temperature control equipment is communicated to connect with the control device, and the control device is by the temperature control equipment control
The low-temperature space warehouse and the temperature in the warehouse of warmer region are made, the video monitoring equipment is communicated to connect with the control device,
The control device obtains the image analysis result that the video monitoring equipment is obtained.
2. device according to claim 1, it is characterised in that the control device is controlled by the temperature control equipment
Temperature in the low-temperature space warehouse is the first temperature, and the control device is controlled described warm by the temperature control equipment
Temperature in area's warehouse is second temperature, and the second temperature is higher than first temperature；
The image analysis result obtained in the control device includes：Laboratory animal quantity in the warmer region warehouse becomes
During change, the temperature in the warmer region warehouse is accordingly adjusted by the temperature control equipment, when in the warmer region warehouse
When laboratory animal quantity increases, the temperature in the warmer region warehouse is reduced by A degree, wherein A by the temperature control equipment
The number * C of the laboratory animal increased in the warehouse of=warmer region, C are for preset temperature value and C is less than the second temperature；When described
When laboratory animal quantity in the warehouse of warmer region is reduced, by the temperature control equipment by the temperature in the warmer region warehouse
B degree is raised, the number * C of the laboratory animal reduced wherein in the warehouse of B=warmer regions.
3. device according to claim 1, it is characterised in that also include：Adjust with the light of control device communication connection
Control device, the smooth regulation device includes：Laser instrument, waveform generator, optical fiber and implantable fine setting optoelectronic pole, the waveform
Generator is connected with laser instrument, and the laser output of the laser instrument finely tunes optoelectronic pole by the optical fiber with the implantable
Connection, the implantable fine setting optoelectronic pole is arranged on the big intracerebral of laboratory animal for having proceeded to light sensation gene.
4. device according to any one of claim 1 to 3, it is characterised in that described device also includes：With the control
The electrical signal collection device of equipment communication connection, the electrical signal collection device includes：Microelectrode and electrical signal collection device, institute
State microelectrode and be arranged on the big intracerebral of laboratory animal, the electrical signal collection device is communicated to connect with the microelectrode, receive institute
State the signal of telecommunication of microelectrode transmission and the signal of telecommunication is analyzed, obtain signal of telecommunication analysis result.
5. device according to claim 4, it is characterised in that the video monitoring equipment is carried out point to the image for gathering
Analysis, obtains the number of laboratory animal in warmer region warehouse data over time, and the delta data is added into image
In analysis result；The control device is according to described image analysis result, the signal of telecommunication analysis result, the warmer region warehouse
In the laser that sends of temperature and the smooth regulation device, it is determined that feelings test result altogether.
6. device according to claim 1, it is characterised in that the low-temperature space warehouse and the warmer region warehouse are row
To learn casing.
7. device according to claim 1, it is characterised in that also include：Dodge gate and controlling organization, the dodge gate sets
It is placed between the low-temperature space warehouse and the warmer region warehouse, the dodge gate is connected with the controlling organization, by the control
Mechanism controls processed are opened and closed, and the controlling organization is communicated to connect with the control device.
8. device according to claim 1, it is characterised in that the video monitoring equipment includes：The photographic head being connected
And video analysis device.
9. device according to claim 1, it is characterised in that can also include：Physiology signal acquisition device, the physiology
The physiological signal and the physiological signal to being gathered of signal pickup assembly collection laboratory animal is analyzed, and obtains physiological signal point
Analysis result.
10. it is a kind of to be total to feelings method of testing, it is characterised in that the common feelings test device described in usage right requirement 1 is to laboratory animal
Common feelings test is carried out, methods described includes：
It is the first temperature that the control device controls the temperature in the low-temperature space warehouse by the temperature control equipment, described
It is second temperature that control device controls temperature in the warmer region warehouse by the temperature control equipment, the second temperature
Higher than first temperature；
The control device controls the first laboratory animal and stops Preset Time in the low-temperature space warehouse；
The control device controls first laboratory animal and is located in the warmer region warehouse and will be multiple with the described first reality
Test the laboratory animal that animal is similar species to be placed in the low-temperature space warehouse；
The control device determines real in the warmer region warehouse according to the image analysis result that the video monitoring equipment is obtained
The variable quantity of animal number is tested, the temperature in the warmer region warehouse is accordingly adjusted by the temperature control equipment, when described
When laboratory animal quantity in the warehouse of warmer region increases, by the temperature control equipment by the temperature in the warmer region warehouse
A degree is reduced, the number * C of the laboratory animal increased wherein in the warehouse of A=warmer regions, C are preset temperature value and C less than described the
Two temperature；When the laboratory animal quantity in the warmer region warehouse is reduced, will be described warm by the temperature control equipment
Temperature in area's warehouse raises B degree, the number * C of the laboratory animal reduced wherein in the warehouse of B=warmer regions；
It is real in the warehouse of warmer region caused by temperature change and the temperature change in warmer region warehouse described in the control device
The variable quantity for testing animal number determines feelings test result altogether.
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| CN201611218183.XA CN106665388B (en) | 2016-12-26 | 2016-12-26 | Feelings test device and method altogether |
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| CN201611218183.XA CN106665388B (en) | 2016-12-26 | 2016-12-26 | Feelings test device and method altogether |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101826197A (en) * | 2009-03-06 | 2010-09-08 | 北京百奥金生物科技有限公司 | Method and systems for monitoring daily activities of animal |
| EP1481585B1 (en) * | 2002-02-14 | 2010-11-10 | Japan Science and Technology Agency | System for measuring heart rate and respiration rate of small animals |
| CN202145792U (en) * | 2011-06-17 | 2012-02-22 | 苏州市冯氏实验动物设备有限公司 | Full-automatic experimental asphyxiator |
| CN205180038U (en) * | 2015-12-09 | 2016-04-27 | 河南中医学院 | Experimental device for be used for simple chronic psychological stress of experimental animals |
| CN205389949U (en) * | 2016-03-22 | 2016-07-27 | 西安医学院 | Experimental animals spontaneous pain data acquisition device |
-
2016
- 2016-12-26 CN CN201611218183.XA patent/CN106665388B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1481585B1 (en) * | 2002-02-14 | 2010-11-10 | Japan Science and Technology Agency | System for measuring heart rate and respiration rate of small animals |
| CN101826197A (en) * | 2009-03-06 | 2010-09-08 | 北京百奥金生物科技有限公司 | Method and systems for monitoring daily activities of animal |
| CN101826197B (en) * | 2009-03-06 | 2012-05-09 | 北京百奥金生物科技有限公司 | Method and systems for monitoring daily activities of animal |
| CN202145792U (en) * | 2011-06-17 | 2012-02-22 | 苏州市冯氏实验动物设备有限公司 | Full-automatic experimental asphyxiator |
| CN205180038U (en) * | 2015-12-09 | 2016-04-27 | 河南中医学院 | Experimental device for be used for simple chronic psychological stress of experimental animals |
| CN205389949U (en) * | 2016-03-22 | 2016-07-27 | 西安医学院 | Experimental animals spontaneous pain data acquisition device |
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